Rw2G049200

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003871
Physical Location & Seq
Forward (+)
76674540 .. 76678232
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049200.1

Sequence Viewer

Length: 1707 bp
ATGTCCTCCAACTTCTCCCTCCCAAGGTGGACGGTATTCCACCGCCGGCAGAACTCACAGAGCGGCGGCGGCCCGAGTGACAGCGACAACAGCAACAACTTTGTGGAAGACAACCCAATCTCACTCTCGACCTCGGTGATCGAGTGCTACGCGTGCACTCAGGTCGGAGTTCCGGTCTTCCACTCGACCAGCTGCGACGGCGCCCACCAGCCCCAATGGGAGGCCTCGGCGGGGTCTTCCCTAGTCCCTATCCAATCCCGGAGCGACCTCAAGAAGGGTCTCCGCCGCAACCGCCTCCCGGTGGGCCCCTTCGGCGAGGTCCTCGACCCGCGCACCAAGCGCGTGCAGAGGTGGAACCGCGCCTTCCTCCTGGCGCGTGGGATGGCCCTCGCCATCGACCCGCTATTCTTCTACGCCCTCTCCATCGGCCGCGGTGGGACTCCCTGCCTCTACATGGACGGCGGCCTTGCGGCAATCGTGACGGTGCTGCGCACATGCGTGGACGCCGTCCACCTTTGCCACCTGTGGCTTCAGTTCCGCCTGGCGTACGTTTCTAGGGAGTCGCTGGTGGTCGGCTGCGGGAAGCTCGTGTGGGACGCACGTGCGATAGCGTCGCACTATGTTCGGTCCCTCAAGGGCTTCTGGTTCGACGCCTTCGTTATTCTTCCGGTTCCTCAGGCTGTGTTCTGGCTTGTTCTTCCAAAATTGATCAGGGAAGAGAAGATTAAAGTGATAATGACAATTCTTCTAGTAATATTTTTATTCCAATTCCTCCCAAAAGTCTACCATAGCATCTGCTTGATGAGAAGAATGGCAAAGGTCACAGGGTACATTTTCGGCACCATTTGGTGGGGTTTTGGCCTCAACCTCATCGCCTACTTCATTGCCTCACATGTTGCTGGGGGATGCTGGTATGTACTTGCTATACAGCGAGTGGCTTCCTGCCTAAAGCAACAGTGTGAATTGGGTGGGAATTGCAATCTCTCTTTGTCATGCTCACAGGAAGTTTGTTACCAATCTCTGATACCGGATTCCATGGAAGCAACTCCATGTGGTAACAATGTGACAAATGTGTTGAGAAAGCCTTCCTGCTTGGATGTTAATGGAACATTCAACTACGGCATCTATCAGTGGGCTCTTCCTGTCATATCTAGCAACTCGCTGTCTATAAAGATCCTTTATCCTATATTTTGGGGCTTAATGACTCTCAGCACATTTGGCAATGATCTTGAACCAACAAGTCACTGGCTAGAAGTGATTTTCAGTATATGCATTGTGCTCAGTGGATTACTGCTCTTCACTTTGCTTATTGGGAACATTCAGGTATTTCTGCACGCGGTTATGGCAAAGAAGAGGAAAATGCAGCTGAGGTGCAGAGACATGGAATGGTGGATGAGAAGAAGGCAGTTGCCATCTGTTTTGCGACGAAGAGTCAGGCATTATGAACGCCATAGATGGGTGACAATGGGGGGAGAAGATGAGATGGATTTGATCAAAGACTTGCCTGAAGGTCTTCGAAGAGAAATCAAACGCCACTTATGCATAGACCTCATAAAGAAGGTACCTCTGTTCCACAACTTGGATGATCTGATTCTTGACAACATATGTGATCGCGTTAGGCCTCTGGTCTTTTCTAGAGATGAAAAGGTACTGAATTCATCTGTGCAAGGATTTTGTCAATACTGTTTTGATGACAATGTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

568

Amino Acids

64.63

Weight (kDa)

9.08

Isoelectric Point (pI)

55.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 158 - 441 1.3e-06 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
Acc65I GGTACC 1 cut(s) 1561
AccB1I GGYRCC 3 cut(s) 200, 839, 1561
AccB7I CCANNNNNTGG 2 cut(s) 849, 1579
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 1 cut(s) 783
AccII CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
AclWI GGATC 1 cut(s) 1168
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 1 cut(s) 1654
AcuI CTGAAG 2 cut(s) 515, 1527
AcvI CACGTG 1 cut(s) 602
AcyI GRCGYC 3 cut(s) 201, 504, 651
AfaI GTAC 5 cut(s) 548, 830, 918, 1563, 1650
AflIII ACRYGT 2 cut(s) 150, 892
AgsI TTSAA 2 cut(s) 1114, 1232
AhdI GACNNNNNGTC 1 cut(s) 1430
AjnI CCWGG 2 cut(s) 369, 540
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 3 cut(s) 192, 586, 1366
AluI AGCT 3 cut(s) 192, 586, 1366
Alw21I GWGCWC 2 cut(s) 158, 1281
Alw26I GTCTC 2 cut(s) 284, 1371
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 1 cut(s) 1168
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 8 cut(s) 70, 222, 304, 384, 427, 463, 859, 1619
ApaI GGGCCC 1 cut(s) 308
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 4 cut(s) 192, 487, 576, 1363
ApoI RAATTY 1 cut(s) 1654
Asp700I GAANNNNTTC 2 cut(s) 1084, 1512
Asp718I GGTACC 1 cut(s) 1561
AspLEI GCGC 6 cut(s) 203, 333, 342, 362, 376, 492
AspS9I GGNCC 6 cut(s) 71, 304, 305, 319, 385, 627
AsuC2I CCSGG 2 cut(s) 259, 299
AsuHPI GGTGA 2 cut(s) 148, 1471
AsuII TTCGAA 1 cut(s) 1516
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 2 cut(s) 319, 627
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 2 cut(s) 158, 308
BaeI ACNNNNGTAYC 2 cut(s) 1553, 1586
BanI GGYRCC 3 cut(s) 200, 839, 1561
BanII GRGCYC 2 cut(s) 308, 1138
BauI CACGAG 1 cut(s) 587
BbrPI CACGTG 1 cut(s) 602
BbsI GAAGAC 4 cut(s) 114, 169, 228, 1505
Bbv12I GWGCWC 2 cut(s) 158, 1281
BbvCI CCTCAGC 1 cut(s) 1367
BbvI GCAGC 4 cut(s) 179, 474, 563, 1375
BccI CCATC 6 cut(s) 376, 401, 431, 1420, 1449, 1477
BceAI ACGGC 4 cut(s) 214, 475, 491, 1135
BcgI CGANNNNNNTGC 4 cut(s) 145, 179, 605, 639
BciT130I CCWGG 2 cut(s) 371, 542
BclI TGATCA 2 cut(s) 708, 1491
BcnI CCSGG 2 cut(s) 259, 299
BcoDI GTCTC 2 cut(s) 284, 1371
BfaI CTAG 6 cut(s) 242, 555, 749, 1152, 1250, 1635
BfoI RGCGCY 1 cut(s) 204
BglI GCCNNNNNGGC 1 cut(s) 312
Bme1390I CCNGG 4 cut(s) 259, 299, 371, 542
Bme18I GGWCC 2 cut(s) 319, 627
BmeRI GACNNNNNGTC 1 cut(s) 1430
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 6 cut(s) 71, 304, 305, 319, 385, 627
BmiI GGNNCC 8 cut(s) 202, 306, 307, 356, 629, 672, 841, 1563
BmrFI CCNGG 4 cut(s) 259, 299, 371, 542
BmsI GCATC 3 cut(s) 801, 896, 1131
BpiI GAAGAC 4 cut(s) 114, 169, 228, 1505
Bpu10I CCTNAGC 1 cut(s) 1367
Bpu14I TTCGAA 1 cut(s) 1516
BpuEI CTTGAG 2 cut(s) 254, 617
BpuMI CCSGG 2 cut(s) 259, 299
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 3 cut(s) 201, 504, 651
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 5 cut(s) 23, 132, 225, 430, 1035
BsaWI WCCGGW 3 cut(s) 172, 667, 1027
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 404, 434
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 1250
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 2 cut(s) 882, 1228
BseBI CCWGG 2 cut(s) 371, 542
BseDI CCNNGG 5 cut(s) 23, 132, 225, 430, 1035
BseGI GGATG 5 cut(s) 387, 911, 1102, 1398, 1588
BseMI GCAATG 2 cut(s) 882, 1228
BseMII CTCAG 5 cut(s) 173, 689, 1222, 1294, 1358
BseNI ACTGG 1 cut(s) 1250
BseSI GKGCMC 2 cut(s) 158, 308
BseX3I CGGCCG 1 cut(s) 427
BseXI GCAGC 4 cut(s) 179, 474, 563, 1375
BseYI CCCAGC 1 cut(s) 899
BsgI GTGCAG 3 cut(s) 365, 1316, 1393
Bsh1236I CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 8 cut(s) 72, 224, 306, 386, 429, 465, 861, 1621
BshNI GGYRCC 3 cut(s) 200, 839, 1561
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 158, 1281
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 6 cut(s) 46, 173, 259, 299, 668, 1028
BsiWI CGTACG 1 cut(s) 546
BslFI GGGAC 4 cut(s) 230, 451, 608, 613
BsmAI GTCTC 2 cut(s) 284, 1371
BsmFI GGGAC 4 cut(s) 230, 451, 608, 613
BsnI GGCC 8 cut(s) 72, 224, 306, 386, 429, 465, 861, 1621
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 1516
Bsp120I GGGCCC 1 cut(s) 304
Bsp1286I GDGCHC 4 cut(s) 158, 308, 1138, 1281
Bsp143I GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
Bsp19I CCATGG 1 cut(s) 1035
BspANI GGCC 8 cut(s) 72, 224, 306, 386, 429, 465, 861, 1621
BspCNI CTCAG 5 cut(s) 172, 688, 1221, 1293, 1359
BspFNI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
BspLI GGNNCC 8 cut(s) 202, 306, 307, 356, 629, 672, 841, 1563
BspPI GGATC 1 cut(s) 1168
BspQI GCTCTTC 2 cut(s) 1143, 1301
BspT104I TTCGAA 1 cut(s) 1516
BspT107I GGYRCC 3 cut(s) 200, 839, 1561
BspTNI GGTCTC 1 cut(s) 284
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 882, 1228
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 1 cut(s) 1250
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 5 cut(s) 23, 132, 225, 430, 1035
BssMI GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
BssNI GRCGYC 3 cut(s) 201, 504, 651
BssSI CACGAG 1 cut(s) 587
BssT1I CCWWGG 2 cut(s) 23, 1035
Bst2BI CACGAG 1 cut(s) 587
Bst2UI CCWGG 2 cut(s) 371, 542
Bst4CI ACNGT 4 cut(s) 34, 484, 957, 1685
Bst6I CTCTTC 6 cut(s) 711, 1143, 1301, 1346, 1423, 1513
BstACI GRCGYC 3 cut(s) 201, 504, 651
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 1516
BstC8I GCNNGC 4 cut(s) 47, 154, 344, 1335
BstDEI CTNAG 5 cut(s) 159, 675, 1208, 1280, 1367
BstDSI CCRYGG 2 cut(s) 430, 1035
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 5 cut(s) 387, 911, 1102, 1398, 1588
BstFNI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 6 cut(s) 203, 333, 342, 362, 376, 492
BstKTI GATC 8 cut(s) 141, 711, 1176, 1228, 1494, 1588, 1612, 1704
BstMAI GTCTC 2 cut(s) 284, 1371
BstMBI GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
BstMCI CGRYCG 1 cut(s) 430
BstNI CCWGG 2 cut(s) 371, 542
BstNSI RCATGY 2 cut(s) 498, 896
BstSCI CCNGG 4 cut(s) 257, 297, 369, 540
BstSLI GKGCMC 2 cut(s) 158, 308
BstUI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
BstV1I GCAGC 4 cut(s) 179, 474, 563, 1375
BstV2I GAAGAC 4 cut(s) 114, 169, 228, 1505
BstX2I RGATCY 1 cut(s) 1173
BstYI RGATCY 1 cut(s) 1173
BstZI CGGCCG 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 8 cut(s) 72, 224, 306, 386, 429, 465, 861, 1621
BtgI CCRYGG 2 cut(s) 430, 1035
BtgZI GCGATG 1 cut(s) 856
BtsCI GGATG 5 cut(s) 387, 911, 1102, 1398, 1588
BtsIMutI CAGTG 4 cut(s) 962, 1136, 1243, 1288
Cac8I GCNNGC 4 cut(s) 47, 154, 344, 1335
CfoI GCGC 6 cut(s) 203, 333, 342, 362, 376, 492
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 6 cut(s) 71, 304, 305, 319, 385, 627
Cfr42I CCGCGG 1 cut(s) 433
CseI GACGC 4 cut(s) 512, 600, 605, 659
Csp6I GTAC 5 cut(s) 547, 829, 917, 1562, 1649
CviAII CATG 7 cut(s) 454, 495, 893, 993, 1036, 1050, 1381
CviQI GTAC 5 cut(s) 547, 829, 917, 1562, 1649
DdeI CTNAG 5 cut(s) 159, 675, 1208, 1280, 1367
DinI GGCGCC 1 cut(s) 202
DpnI GATC 8 cut(s) 140, 710, 1175, 1227, 1493, 1587, 1611, 1703
DpnII GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
DriI GACNNNNNGTC 1 cut(s) 1430
EaeI YGGCCR 1 cut(s) 427
EagI CGGCCG 1 cut(s) 427
Eam1104I CTCTTC 6 cut(s) 711, 1143, 1301, 1346, 1423, 1513
Eam1105I GACNNNNNGTC 1 cut(s) 1430
EarI CTCTTC 6 cut(s) 711, 1143, 1301, 1346, 1423, 1513
EciI GGCGGA 2 cut(s) 272, 527
EclXI CGGCCG 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 23, 1035
Eco147I AGGCCT 2 cut(s) 224, 1621
Eco24I GRGCYC 2 cut(s) 308, 1138
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 2 cut(s) 319, 627
Eco52I CGGCCG 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 515, 1527
Eco72I CACGTG 1 cut(s) 602
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 73
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 305, 319
EcoRI GAATTC 1 cut(s) 1654
EcoRII CCWGG 2 cut(s) 369, 540
EcoT14I CCWWGG 2 cut(s) 23, 1035
EcoT22I ATGCAT 2 cut(s) 1274, 1544
EcoT38I GRGCYC 2 cut(s) 308, 1138
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 23, 1035
FaeI CATG 7 cut(s) 457, 498, 896, 996, 1039, 1053, 1384
FaqI GGGAC 4 cut(s) 230, 451, 608, 613
FatI CATG 7 cut(s) 453, 494, 892, 992, 1035, 1049, 1380
FauI CCCGC 4 cut(s) 223, 336, 408, 572
FauNDI CATATG 1 cut(s) 1604
FbaI TGATCA 2 cut(s) 708, 1491
FblI GTMKAC 1 cut(s) 783
FokI GGATG 5 cut(s) 394, 918, 1109, 1405, 1595
FriOI GRGCYC 2 cut(s) 308, 1138
FspBI CTAG 6 cut(s) 242, 555, 749, 1152, 1250, 1635
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 6 cut(s) 202, 332, 341, 361, 375, 491
GsaI CCCAGC 1 cut(s) 903
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 8 cut(s) 72, 224, 306, 386, 429, 465, 861, 1621
HapII CCGG 6 cut(s) 46, 173, 259, 299, 668, 1028
HgaI GACGC 4 cut(s) 512, 600, 605, 659
HhaI GCGC 6 cut(s) 203, 333, 342, 362, 376, 492
Hin1I GRCGYC 3 cut(s) 201, 504, 651
Hin1II CATG 7 cut(s) 457, 498, 896, 996, 1039, 1053, 1384
Hin6I GCGC 6 cut(s) 201, 331, 340, 360, 374, 490
HinP1I GCGC 6 cut(s) 201, 331, 340, 360, 374, 490
HinfI GANTC 6 cut(s) 439, 560, 1031, 1204, 1431, 1591
HpaII CCGG 6 cut(s) 46, 173, 259, 299, 668, 1028
HphI GGTGA 2 cut(s) 148, 1471
Hpy166II GTNNAC 5 cut(s) 30, 156, 502, 511, 784
Hpy188I TCNGA 3 cut(s) 167, 1023, 1590
Hpy188III TCNNGA 6 cut(s) 127, 271, 478, 1229, 1595, 1635
Hpy8I GTNNAC 5 cut(s) 30, 156, 502, 511, 784
Hpy99I CGWCG 4 cut(s) 200, 616, 653, 1428
HpyAV CCTTC 8 cut(s) 268, 319, 373, 664, 1095, 1395, 1502, 1552
HpyCH4III ACNGT 4 cut(s) 34, 484, 957, 1685
HpyCH4IV ACGT 2 cut(s) 549, 601
HpyCH4V TGCA 9 cut(s) 156, 346, 978, 1272, 1333, 1363, 1374, 1542, 1666
HpyF3I CTNAG 5 cut(s) 159, 675, 1208, 1280, 1367
HpySE526I ACGT 2 cut(s) 549, 601
Hsp92I GRCGYC 3 cut(s) 201, 504, 651
Hsp92II CATG 7 cut(s) 457, 498, 896, 996, 1039, 1053, 1384
HspAI GCGC 6 cut(s) 201, 331, 340, 360, 374, 490
KasI GGCGCC 1 cut(s) 200
KpnI GGTACC 1 cut(s) 1565
KroI GCCGGC 1 cut(s) 45
KroNI GCCGGC 1 cut(s) 47
Ksp22I TGATCA 2 cut(s) 708, 1491
KspI CCGCGG 1 cut(s) 433
Kzo9I GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
LguI GCTCTTC 2 cut(s) 1143, 1301
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 4 cut(s) 179, 474, 563, 1375
LweI GCATC 3 cut(s) 801, 896, 1131
MaeI CTAG 6 cut(s) 242, 555, 749, 1152, 1250, 1635
MaeII ACGT 2 cut(s) 549, 601
MaeIII GTNAC 8 cut(s) 77, 478, 820, 1010, 1055, 1063, 1241, 1459
MalI GATC 8 cut(s) 140, 710, 1175, 1227, 1493, 1587, 1611, 1703
MbiI CCGCTC 1 cut(s) 63
MboI GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
MflI RGATCY 1 cut(s) 1173
MhlI GDGCHC 4 cut(s) 158, 308, 1138, 1281
MluCI AATT 6 cut(s) 704, 741, 767, 962, 973, 1654
MluI ACGCGT 1 cut(s) 150
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 4 cut(s) 433, 569, 1198, 1440
MmeI TCCRAC 2 cut(s) 33, 145
Mph1103I ATGCAT 2 cut(s) 1274, 1544
MroNI GCCGGC 1 cut(s) 45
MroXI GAANNNNTTC 2 cut(s) 1084, 1512
MseI TTAA 3 cut(s) 726, 1101, 1199
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 3 cut(s) 192, 432, 1366
MspI CCGG 6 cut(s) 46, 173, 259, 299, 668, 1028
MspR9I CCNGG 4 cut(s) 259, 299, 371, 542
MvaI CCWGG 2 cut(s) 371, 542
MvnI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
NaeI GCCGGC 1 cut(s) 47
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 2 cut(s) 259, 299
NcoI CCATGG 1 cut(s) 1035
NdeI CATATG 1 cut(s) 1604
NdeII GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
NgoMIV GCCGGC 1 cut(s) 45
NlaIII CATG 7 cut(s) 457, 498, 896, 996, 1039, 1053, 1384
NlaIV GGNNCC 8 cut(s) 202, 306, 307, 356, 629, 672, 841, 1563
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 6 cut(s) 77, 478, 820, 1063, 1241, 1459
NsbI TGCGCA 1 cut(s) 491
NsiI ATGCAT 2 cut(s) 1274, 1544
NspI RCATGY 2 cut(s) 498, 896
NspV TTCGAA 1 cut(s) 1516
OliI CACNNNNGTG 1 cut(s) 497
PceI AGGCCT 2 cut(s) 224, 1621
PciI ACATGT 1 cut(s) 892
PciSI GCTCTTC 2 cut(s) 1143, 1301
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PdiI GCCGGC 1 cut(s) 47
PdmI GAANNNNTTC 2 cut(s) 1084, 1512
PfeI GAWTC 2 cut(s) 1031, 1591
Pfl23II CGTACG 1 cut(s) 546
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 2 cut(s) 849, 1579
PfoI TCCNGGA 1 cut(s) 257
PleI GAGTC 4 cut(s) 433, 568, 1198, 1439
PluTI GGCGCC 1 cut(s) 204
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 4 cut(s) 433, 568, 1198, 1439
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 319
PscI ACATGT 1 cut(s) 892
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 369, 540
PspCI CACGTG 1 cut(s) 602
PspFI CCCAGC 1 cut(s) 899
PspGI CCWGG 2 cut(s) 369, 540
PspLI CGTACG 1 cut(s) 546
PspN4I GGNNCC 8 cut(s) 202, 306, 307, 356, 629, 672, 841, 1563
PspOMI GGGCCC 1 cut(s) 304
PspPI GGNCC 6 cut(s) 71, 304, 305, 319, 385, 627
PspPPI RGGWCCY 1 cut(s) 319
PsuI RGATCY 1 cut(s) 1173
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 2 cut(s) 192, 1366
RsaI GTAC 5 cut(s) 548, 830, 918, 1563, 1650
RsaNI GTAC 5 cut(s) 547, 829, 917, 1562, 1649
RseI CAYNNNNRTG 1 cut(s) 497
SacII CCGCGG 1 cut(s) 433
SapI GCTCTTC 2 cut(s) 1143, 1301
SaqAI TTAA 3 cut(s) 726, 1101, 1199
Sau3AI GATC 8 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 1701
Sau96I GGNCC 6 cut(s) 71, 304, 305, 319, 385, 627
SchI GAGTC 4 cut(s) 433, 569, 1198, 1440
ScrFI CCNGG 4 cut(s) 259, 299, 371, 542
SduI GDGCHC 4 cut(s) 158, 308, 1138, 1281
SfaNI GCATC 3 cut(s) 801, 896, 1131
SfoI GGCGCC 1 cut(s) 202
Sfr303I CCGCGG 1 cut(s) 433
SfuI TTCGAA 1 cut(s) 1516
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 2 cut(s) 319, 627
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 269, 632
SmoI CTYRAG 2 cut(s) 269, 632
Sse9I AATT 6 cut(s) 704, 741, 767, 962, 973, 1654
SseBI AGGCCT 2 cut(s) 224, 1621
SspDI GGCGCC 1 cut(s) 200
SspI AATATT 1 cut(s) 756
SspMI CTAG 6 cut(s) 242, 555, 749, 1152, 1250, 1635
StuI AGGCCT 2 cut(s) 224, 1621
StyD4I CCNGG 4 cut(s) 257, 297, 369, 540
StyI CCWWGG 2 cut(s) 23, 1035
TaaI ACNGT 4 cut(s) 34, 484, 957, 1685
TaiI ACGT 2 cut(s) 552, 604
TaqI TCGA 7 cut(s) 128, 141, 185, 324, 396, 648, 1516
TaqII GACCGA 1 cut(s) 615
TasI AATT 6 cut(s) 704, 741, 767, 962, 973, 1654
TatI WGTACW 1 cut(s) 916
TauI GCSGC 7 cut(s) 66, 69, 72, 288, 432, 465, 473
TfiI GAWTC 2 cut(s) 1031, 1591
Tru1I TTAA 3 cut(s) 726, 1101, 1199
Tru9I TTAA 3 cut(s) 726, 1101, 1199
TscAI CASTG 4 cut(s) 962, 1136, 1250, 1288
TseFI GTSAC 6 cut(s) 77, 478, 820, 1063, 1241, 1459
TseI GCWGC 4 cut(s) 192, 487, 576, 1363
Tsp45I GTSAC 6 cut(s) 77, 478, 820, 1063, 1241, 1459
TspDTI ATGAA 4 cut(s) 871, 1458, 1647, 1656
TspRI CASTG 4 cut(s) 962, 1136, 1250, 1288
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 2 cut(s) 849, 1579
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 319, 627
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 1 cut(s) 1654
XbaI TCTAGA 1 cut(s) 1634
XceI RCATGY 2 cut(s) 498, 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 1242
XmiI GTMKAC 1 cut(s) 783
XmnI GAANNNNTTC 2 cut(s) 1084, 1512
XspI CTAG 6 cut(s) 242, 555, 749, 1152, 1250, 1635
Zsp2I ATGCAT 2 cut(s) 1274, 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.