Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003871

Physical Location & Seq

Genomic Coordinates Reverse (-)

4450550 .. 4454103

Gene Structure 3554 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1056400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGTCCTCCTCCCAGTTCTTCCTCCCAAGGTGGACCCGAGTTCTCCGGCGCCAGAACTCCCAGAGCGACGACACCGACAACACCCACAACTCCAACAACATCGAAGACATCATCAACACCCCAATCATCTCCAACTCCGTCGAATGCTACGCCTGCACTCAGGTGGGGGTCCCAGCCTTTCACTCCACTAGCTGCGACGCCGCCCACCAGCCCCAATGGGAGGCCTCCGCCGGCTCTTCCCTGATCCCCATCCAATCCCGCTCCGATCTCAAAAAGGGTCTCTCCCGCCACCGCCCAGCCAGCCCCTTCGGCCCCGTACTTGACCCCCGCAGCAAGCGCGTCCAGCGCTGGAACCGCGCGTTCCTCCTCGCGCGTGGGATGGCGCTGGCCGTCGACCCGCTCTTCTTCTACGCCCTCTCCATTGGAAGAAGCGGGACGCCCTGCCTATACATGGACGGCGGGCTAGCAGCAATTGTCACCGTGCTCCGCACATGCGTGGACGCCGTGCACCTGTGCCACCTGTGGCTACAGTTCCGGCTGGCGTACGTGTCGAGGGAGTCTCTGGTGGTCGGCTGCGGGAAGCTCGTGTGGGACGCACGTGCGATCTCCTCCCACTACCTTCGATCTCTCAAAGGTTTCTGGTTCGACGCCTTCGTCATCCTCCCCGTTCCCCAGGCAGTATTTTGGCTGGTTCTTCCAAAATTGATAAAAGAAGAGAAGATTAAGGTGATCATGACCATTCTTCTCTTAATTTTTTTGTTCCAATTCCTCCCAAAAGTCTACCACAGCATCTCCTTGATGAGAAGAATGCAAAAGGTCACAGGCTACATCTTTGGCACCATTTGGTGGGGCTTTGGCCTCAACCTCATCGCCTACTTCATTGCCTCTCACGTTGCTGGGGGATGCTGGTATGTGCTCGCGATTCAGCGTGTAGCTTCGTGCCTAAGGCAACATTGTGACCAAAATGTCAACTGCAACCTCTCTTTGTCTTGCTCGGACGAAGTTTGCTACCAGTTTTTGTTACCAGCCTCCACGAGTGGAAGCACTTGTGGAGGCAACACAACAGCAAGCGTGATGAGAAAGCCGCCACTCTGCCTGGATGTCAATGGCACATTTAACTATGGAATCTATCAGTTGGCTCTTCCTGTCATTTCTAGCAACTCCCTTGCTATTAAGATCCTTTATCCCATCTTTTGGGGTTTAATGACTCTTAGTACATTTGGGAATGATCTTGAACCTACAAGTCACTGGTTAGAAGTGATTTTCAGCATATGCATTGTGCTTAGTGGCTTGCTGCTATTCACTTTGTTGATCGGGAACATTCAGGTATTTCTCCACGCCGTTATGGCAAAGAAAAGGAAGATGCAGCTGAGATGCAGAGACATGGAATGGTGGATGAAGCGGAGGCAGTTGCCTTCTGGATTGAGACGAAGAGTACGCCATTACGAAAGGCATAGATGGGTGACAATGGGGGGAGAAGATGAGATGGATTTGATCAAAGACTTGCCGGAAGGGCTCCGGAGGGAGATTAAGCGCCATTTATGCTTAGAACTCATCAAAAAGGTTCCCCTGTTCGACAACTTGGATGATCTTATTCTTGACAACATTTGTGATAGGGTTAGGCCTCTGGTCTTCTCCAAAGATGAAAAGATAATCAGAGAAGGAGATCCTGTGCCGAGGATGCTATTTATTGTGCGTGGACGCATTAAGCGTACCCAAGGCCTAAGCAAAGGCATGGTGGGAACGAATGTGCTTGAACCGGGAGGGTTTTTTGGCGATGAGCTGCTCTCCTGGTGCCTTCGCCGGCCATTCATAGACCGCCTTCCAGCCTCATCTGCGACATTCACCTGCATTGAATCAACAGAAGCATTTGGCCTCAATGCAGTCGATCTGAGATACATCACGGATCACTTCCGTTACAAATTTGCCAACGAGAGGCTTAAGCGAACTGCGAGATACTACTCTTCGAATTGGAGAACATGGGCAGCTGTGAACATACAATTTACTTGGCGTCGCTACAGGTTGAGGACTAATCGAGGTCCGGTGATTCCTGTGATTCCAAATGGAGGCTCTGAGCGGAAGCTGTTGCAGTATGCTGCAATGTTCATGTCACTTAGGCCACACGATCACCTTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

713

Amino Acids

81.48

Weight (kDa)

9.58

Isoelectric Point (pI)

54.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	157 - 442	2.1e-06	Ion transport protein
cNMP_binding	PF00027	544 - 632	3.6e-06	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15410 AT5G15410
fragaria_vesca	FvH4_6g47520 FvH4_6g47520 FvH4_7g26890
malus_domestica	MD07G1248200.v1.1 MD09G1061000.v1.1 MD17G1056400.v1.1
prunus_persica	Prupe.2G272100_v2.0.a1 Prupe.3G259100_v2.0.a1
pyrus_communis	pycom07g22190 pycom111g05130 pycom17g05550
rosa_chinensis	RchiOBHm_Chr1g0373561 RchiOBHm_Chr2g0166581
rosa_laevigata	RLG00000021630 RLG00000026815
rosa_multiflora	Rmu_sc0002860.1_g000003 Rmu_sc0008222.1_g000009
rosa_roxburghii	Rroxscaffold_2G00084740 Rroxscaffold_4G00284140
rosa_rugosa	Rorug01G0378300 Rorug02G0524300 Rorug02G0524400
rosa_samantha	Rh1AG387700 Rh1BG352000 Rh1CG364700 Rh1DG382400 Rh2AG590200 Rh2BG602300 Rh2CG573000 Rh2DG612900
rosa_wichuraiana	Rw1G034770 Rw2G049200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1856
AasI	GACNNNNNNGTC	2 cut(s)	653, 965
Acc36I	ACCTGC	1 cut(s)	1856
AccB1I	GGYRCC	3 cut(s)	48, 836, 1794
AccB7I	CCANNNNNTGG	2 cut(s)	846, 1194
AccBSI	CCGCTC	3 cut(s)	261, 400, 2077
AccI	GTMKAC	2 cut(s)	393, 780
AccII	CGCG	6 cut(s)	339, 357, 359, 371, 373, 920
AccIII	TCCGGA	1 cut(s)	1518
AclWI	GGATC	4 cut(s)	238, 1171, 1661, 1914
AcoI	YGGCCR	2 cut(s)	387, 1805
AcsI	RAATTY	1 cut(s)	1922
AcvI	CACGTG	1 cut(s)	599
AcyI	GRCGYC	6 cut(s)	49, 198, 437, 501, 648, 2011
AfaI	GTAC	5 cut(s)	318, 545, 1216, 1437, 1714
AfeI	AGCGCT	1 cut(s)	347
AfiI	CCNNNNNNNGG	6 cut(s)	220, 451, 846, 1194, 1935, 2066
AflII	CTTAAG	1 cut(s)	1940
AflIII	ACRYGT	1 cut(s)	546
AgsI	TTSAA	4 cut(s)	1235, 1757, 1856, 2135
AjnI	CCWGG	3 cut(s)	672, 1095, 1790
AleI	CACNNNNGTG	2 cut(s)	161, 494
AluBI	AGCT	7 cut(s)	192, 583, 935, 1369, 1783, 1988, 2083
AluI	AGCT	7 cut(s)	192, 583, 935, 1369, 1783, 1988, 2083
Alw21I	GWGCWC	3 cut(s)	486, 510, 918
Alw26I	GTCTC	4 cut(s)	284, 564, 1374, 1420
Alw44I	GTGCAC	1 cut(s)	506
AlwI	GGATC	4 cut(s)	238, 1171, 1661, 1914
Ama87I	CYCGRG	1 cut(s)	36
Aor13HI	TCCGGA	1 cut(s)	1518
Aor51HI	AGCGCT	1 cut(s)	347
AoxI	GGCC	9 cut(s)	222, 310, 387, 856, 1622, 1720, 1805, 1873, 2117
ApaLI	GTGCAC	1 cut(s)	506
ApeKI	GCWGC	9 cut(s)	192, 330, 467, 573, 1294, 1366, 1783, 1985, 2096
ApoI	RAATTY	1 cut(s)	1922
AspLEI	GCGC	7 cut(s)	51, 339, 348, 359, 373, 385, 1536
AspS9I	GGNCC	4 cut(s)	33, 169, 311, 2039
AsuC2I	CCSGG	1 cut(s)	1761
AsuHPI	GGTGA	6 cut(s)	469, 739, 1474, 1837, 2056, 2120
AsuII	TTCGAA	1 cut(s)	1967
AsuNHI	GCTAGC	1 cut(s)	463
AvaI	CYCGRG	1 cut(s)	36
AvaII	GGWCC	3 cut(s)	33, 169, 2039
AxyI	CCTNAGG	1 cut(s)	944
BaeGI	GKGCMC	1 cut(s)	510
BanI	GGYRCC	3 cut(s)	48, 836, 1794
BanII	GRGCYC	1 cut(s)	1518
BauI	CACGAG	2 cut(s)	584, 1033
BbrPI	CACGTG	1 cut(s)	599
BbsI	GAAGAC	2 cut(s)	111, 1624
Bbv12I	GWGCWC	3 cut(s)	486, 510, 918
BbvI	GCAGC	9 cut(s)	179, 342, 479, 560, 1281, 1378, 1770, 1997, 2083
BccI	CCATC	5 cut(s)	257, 373, 1196, 1452, 1480
BceAI	ACGGC	4 cut(s)	374, 472, 488, 1325
BciT130I	CCWGG	3 cut(s)	674, 1097, 1792
BclI	TGATCA	2 cut(s)	729, 1494
BcnI	CCSGG	1 cut(s)	1761
BcoDI	GTCTC	4 cut(s)	284, 564, 1374, 1420
BfaI	CTAG	3 cut(s)	189, 464, 1155
BfmI	CTRYAG	2 cut(s)	527, 2017
BfoI	RGCGCY	4 cut(s)	52, 349, 386, 1537
BfrI	CTTAAG	1 cut(s)	1940
BfuAI	ACCTGC	1 cut(s)	1856
BglI	GCCNNNNNGGC	3 cut(s)	309, 1346, 1513
Bme1390I	CCNGG	4 cut(s)	674, 1097, 1761, 1792
Bme18I	GGWCC	3 cut(s)	33, 169, 2039
BmeT110I	CYCGRG	1 cut(s)	36
BmgT120I	GGNCC	4 cut(s)	33, 169, 311, 2039
BmrFI	CCNGG	4 cut(s)	674, 1097, 1761, 1792
BmrI	ACTGGG	1 cut(s)	7
BmsI	GCATC	5 cut(s)	798, 893, 1353, 1364, 1671
BmtI	GCTAGC	1 cut(s)	467
BmuI	ACTGGG	1 cut(s)	7
BpiI	GAAGAC	2 cut(s)	111, 1624
BplI	GAGNNNNNCTC	4 cut(s)	544, 576, 1772, 1804
Bpu10I	CCTNAGC	1 cut(s)	1724
Bpu14I	TTCGAA	1 cut(s)	1967
BpuMI	CCSGG	1 cut(s)	1761
BsaAI	YACGTR	2 cut(s)	547, 599
BsaBI	GATNNNNATC	1 cut(s)	248
BsaHI	GRCGYC	6 cut(s)	49, 198, 437, 501, 648, 2011
BsaI	GGTCTC	1 cut(s)	284
BsaJI	CCNNGG	4 cut(s)	26, 672, 1676, 1717
BsaWI	WCCGGW	2 cut(s)	1518, 2041
BsaXI	ACNNNNNCTCC	6 cut(s)	401, 431, 776, 806, 1656, 1686
Bsc4I	CCNNNNNNNGG	6 cut(s)	220, 451, 846, 1194, 1935, 2066
Bse118I	RCCGGY	2 cut(s)	230, 1803
Bse1I	ACTGG	3 cut(s)	13, 1012, 1253
Bse21I	CCTNAGG	1 cut(s)	944
Bse3DI	GCAATG	2 cut(s)	879, 2106
Bse8I	GATNNNNATC	1 cut(s)	248
BseAI	TCCGGA	1 cut(s)	1518
BseBI	CCWGG	3 cut(s)	674, 1097, 1792
BseDI	CCNNGG	4 cut(s)	26, 672, 1676, 1717
BseGI	GGATG	8 cut(s)	249, 384, 657, 908, 1105, 1401, 1591, 1686
BseJI	GATNNNNATC	1 cut(s)	248
BseLI	CCNNNNNNNGG	6 cut(s)	220, 451, 846, 1194, 1935, 2066
BseMI	GCAATG	2 cut(s)	879, 2106
BseMII	CTCAG	4 cut(s)	173, 1361, 1883, 2064
BseNI	ACTGG	3 cut(s)	13, 1012, 1253
BseRI	GAGGAG	2 cut(s)	356, 598
BseSI	GKGCMC	1 cut(s)	510
BseXI	GCAGC	9 cut(s)	179, 342, 479, 560, 1281, 1378, 1770, 1997, 2083
BseYI	CCCAGC	3 cut(s)	172, 295, 896
BsgI	GTGCAG	1 cut(s)	139
Bsh1236I	CGCG	6 cut(s)	339, 357, 359, 371, 373, 920
BshFI	GGCC	9 cut(s)	224, 312, 389, 858, 1624, 1722, 1807, 1875, 2119
BshNI	GGYRCC	3 cut(s)	48, 836, 1794
BsiHKAI	GWGCWC	3 cut(s)	486, 510, 918
BsiHKCI	CYCGRG	1 cut(s)	36
BsiSI	CCGG	8 cut(s)	46, 231, 535, 1508, 1519, 1760, 1804, 2042
BsiWI	CGTACG	1 cut(s)	543
BslFI	GGGAC	3 cut(s)	155, 448, 605
BslI	CCNNNNNNNGG	6 cut(s)	220, 451, 846, 1194, 1935, 2066
BsmAI	GTCTC	4 cut(s)	284, 564, 1374, 1420
BsmBI	CGTCTC	1 cut(s)	1420
BsmFI	GGGAC	3 cut(s)	155, 448, 605
BsmI	GAATGC	2 cut(s)	149, 813
BsnI	GGCC	9 cut(s)	224, 312, 389, 858, 1624, 1722, 1807, 1875, 2119
Bso31I	GGTCTC	1 cut(s)	284
BsoBI	CYCGRG	1 cut(s)	36
Bsp119I	TTCGAA	1 cut(s)	1967
Bsp1286I	GDGCHC	4 cut(s)	486, 510, 918, 1518
Bsp13I	TCCGGA	1 cut(s)	1518
Bsp68I	TCGCGA	1 cut(s)	920
BspANI	GGCC	9 cut(s)	224, 312, 389, 858, 1624, 1722, 1807, 1875, 2119
BspCNI	CTCAG	4 cut(s)	172, 1362, 1884, 2065
BspEI	TCCGGA	1 cut(s)	1518
BspFNI	CGCG	6 cut(s)	339, 357, 359, 371, 373, 920
BspHI	TCATGA	1 cut(s)	732
BspMI	ACCTGC	1 cut(s)	1856
BspOI	GCTAGC	1 cut(s)	467
BspPI	GGATC	4 cut(s)	238, 1171, 1661, 1914
BspQI	GCTCTTC	3 cut(s)	241, 407, 1146
BspT104I	TTCGAA	1 cut(s)	1967
BspT107I	GGYRCC	3 cut(s)	48, 836, 1794
BspTI	CTTAAG	1 cut(s)	1940
BspTNI	GGTCTC	1 cut(s)	284
BsrBI	CCGCTC	3 cut(s)	261, 400, 2077
BsrDI	GCAATG	2 cut(s)	879, 2106
BsrFI	RCCGGY	2 cut(s)	230, 1803
BsrI	ACTGG	3 cut(s)	13, 1012, 1253
BssAI	RCCGGY	2 cut(s)	230, 1803
BssECI	CCNNGG	4 cut(s)	26, 672, 1676, 1717
BssNI	GRCGYC	6 cut(s)	49, 198, 437, 501, 648, 2011
BssSI	CACGAG	2 cut(s)	584, 1033
BssT1I	CCWWGG	2 cut(s)	26, 1717
Bst2BI	CACGAG	2 cut(s)	584, 1033
Bst2UI	CCWGG	3 cut(s)	674, 1097, 1792
Bst4CI	ACNGT	2 cut(s)	481, 531
Bst6I	CTCTTC	6 cut(s)	241, 407, 708, 1146, 1426, 1969
BstACI	GRCGYC	6 cut(s)	49, 198, 437, 501, 648, 2011
BstAFI	CTTAAG	1 cut(s)	1940
BstBAI	YACGTR	2 cut(s)	547, 599
BstBI	TTCGAA	1 cut(s)	1967
BstF5I	GGATG	8 cut(s)	249, 384, 657, 908, 1105, 1401, 1591, 1686
BstFNI	CGCG	6 cut(s)	339, 357, 359, 371, 373, 920
BstH2I	RGCGCY	4 cut(s)	52, 349, 386, 1537
BstHHI	GCGC	7 cut(s)	51, 339, 348, 359, 373, 385, 1536
BstMAI	GTCTC	4 cut(s)	284, 564, 1374, 1420
BstNI	CCWGG	3 cut(s)	674, 1097, 1792
BstNSI	RCATGY	1 cut(s)	495
BstSCI	CCNGG	4 cut(s)	672, 1095, 1759, 1790
BstSFI	CTRYAG	2 cut(s)	527, 2017
BstSLI	GKGCMC	1 cut(s)	510
BstUI	CGCG	6 cut(s)	339, 357, 359, 371, 373, 920
BstV1I	GCAGC	9 cut(s)	179, 342, 479, 560, 1281, 1378, 1770, 1997, 2083
BstV2I	GAAGAC	2 cut(s)	111, 1624
BstX2I	RGATCY	2 cut(s)	1176, 1666
BstYI	RGATCY	2 cut(s)	1176, 1666
Bsu36I	CCTNAGG	1 cut(s)	944
BsuRI	GGCC	9 cut(s)	224, 312, 389, 858, 1624, 1722, 1807, 1875, 2119
BtgZI	GCGATG	2 cut(s)	853, 1791
BtsCI	GGATG	8 cut(s)	249, 384, 657, 908, 1105, 1401, 1591, 1686
BtsIMutI	CAGTG	1 cut(s)	1246
BtuMI	TCGCGA	1 cut(s)	920
BveI	ACCTGC	1 cut(s)	1856
CciI	TCATGA	1 cut(s)	732
CfoI	GCGC	7 cut(s)	51, 339, 348, 359, 373, 385, 1536
Cfr10I	RCCGGY	2 cut(s)	230, 1803
Cfr13I	GGNCC	4 cut(s)	33, 169, 311, 2039
CseI	GACGC	8 cut(s)	206, 328, 445, 509, 602, 656, 1710, 2000
Csp6I	GTAC	5 cut(s)	317, 544, 1215, 1436, 1713
CviAII	CATG	7 cut(s)	451, 492, 733, 1384, 1735, 1980, 2107
CviQI	GTAC	5 cut(s)	317, 544, 1215, 1436, 1713
DinI	GGCGCC	1 cut(s)	50
DrdI	GACNNNNNNGTC	2 cut(s)	653, 965
DseDI	GACNNNNNNGTC	2 cut(s)	653, 965
EaeI	YGGCCR	2 cut(s)	387, 1805
Eam1104I	CTCTTC	6 cut(s)	241, 407, 708, 1146, 1426, 1969
EarI	CTCTTC	6 cut(s)	241, 407, 708, 1146, 1426, 1969
EciI	GGCGGA	1 cut(s)	217
Eco130I	CCWWGG	2 cut(s)	26, 1717
Eco147I	AGGCCT	3 cut(s)	224, 1624, 1722
Eco24I	GRGCYC	1 cut(s)	1518
Eco31I	GGTCTC	1 cut(s)	284
Eco47I	GGWCC	3 cut(s)	33, 169, 2039
Eco47III	AGCGCT	1 cut(s)	347
Eco72I	CACGTG	1 cut(s)	599
Eco81I	CCTNAGG	1 cut(s)	944
Eco88I	CYCGRG	1 cut(s)	36
EcoO109I	RGGNCCY	1 cut(s)	169
EcoRII	CCWGG	3 cut(s)	672, 1095, 1790
EcoT14I	CCWWGG	2 cut(s)	26, 1717
EcoT22I	ATGCAT	1 cut(s)	1277
EcoT38I	GRGCYC	1 cut(s)	1518
EgeI	GGCGCC	1 cut(s)	50
EheI	GGCGCC	1 cut(s)	50
ErhI	CCWWGG	2 cut(s)	26, 1717
Esp3I	CGTCTC	1 cut(s)	1420
FaeI	CATG	7 cut(s)	454, 495, 736, 1387, 1738, 1983, 2110
FaqI	GGGAC	3 cut(s)	155, 448, 605
FatI	CATG	7 cut(s)	450, 491, 732, 1383, 1734, 1979, 2106
FauI	CCCGC	7 cut(s)	266, 293, 335, 405, 425, 452, 569
FauNDI	CATATG	1 cut(s)	1271
FbaI	TGATCA	2 cut(s)	729, 1494
FblI	GTMKAC	2 cut(s)	393, 780
FokI	GGATG	8 cut(s)	236, 391, 644, 915, 1112, 1408, 1598, 1693
FriOI	GRGCYC	1 cut(s)	1518
FspBI	CTAG	3 cut(s)	189, 464, 1155
GlaI	GCGC	7 cut(s)	50, 338, 347, 358, 372, 384, 1535
GsaI	CCCAGC	3 cut(s)	176, 299, 900
HaeII	RGCGCY	4 cut(s)	52, 349, 386, 1537
HaeIII	GGCC	9 cut(s)	224, 312, 389, 858, 1624, 1722, 1807, 1875, 2119
HapII	CCGG	8 cut(s)	46, 231, 535, 1508, 1519, 1760, 1804, 2042
HgaI	GACGC	8 cut(s)	206, 328, 445, 509, 602, 656, 1710, 2000
HhaI	GCGC	7 cut(s)	51, 339, 348, 359, 373, 385, 1536
Hin1I	GRCGYC	6 cut(s)	49, 198, 437, 501, 648, 2011
Hin1II	CATG	7 cut(s)	454, 495, 736, 1387, 1738, 1983, 2110
Hin6I	GCGC	7 cut(s)	49, 337, 346, 357, 371, 383, 1534
HinP1I	GCGC	7 cut(s)	49, 337, 346, 357, 371, 383, 1534
HincII	GTYRAC	2 cut(s)	394, 970
HindII	GTYRAC	2 cut(s)	394, 970
HinfI	GANTC	7 cut(s)	557, 922, 1125, 1207, 1856, 2047, 2056
HpaII	CCGG	8 cut(s)	46, 231, 535, 1508, 1519, 1760, 1804, 2042
HphI	GGTGA	6 cut(s)	469, 739, 1474, 1837, 2056, 2120
Hpy166II	GTNNAC	8 cut(s)	33, 394, 499, 508, 781, 970, 1700, 1993
Hpy188I	TCNGA	5 cut(s)	265, 997, 1658, 1893, 2074
Hpy188III	TCNNGA	7 cut(s)	733, 919, 1232, 1315, 1419, 1519, 1598
Hpy8I	GTNNAC	8 cut(s)	33, 394, 499, 508, 781, 970, 1700, 1993
Hpy99I	CGWCG	6 cut(s)	71, 143, 200, 395, 650, 2016
HpyAV	CCTTC	8 cut(s)	316, 629, 661, 1425, 1505, 1655, 1808, 1832
HpyCH4III	ACNGT	2 cut(s)	481, 531
HpyCH4IV	ACGT	3 cut(s)	546, 598, 891
HpySE526I	ACGT	3 cut(s)	546, 598, 891
Hsp92I	GRCGYC	6 cut(s)	49, 198, 437, 501, 648, 2011
Hsp92II	CATG	7 cut(s)	454, 495, 736, 1387, 1738, 1983, 2110
HspAI	GCGC	7 cut(s)	49, 337, 346, 357, 371, 383, 1534
KasI	GGCGCC	1 cut(s)	48
KflI	GGGWCCC	1 cut(s)	169
Kpn2I	TCCGGA	1 cut(s)	1518
KroI	GCCGGC	2 cut(s)	230, 1803
KroNI	GCCGGC	2 cut(s)	232, 1805
Ksp22I	TGATCA	2 cut(s)	729, 1494
LguI	GCTCTTC	3 cut(s)	241, 407, 1146
LmnI	GCTCC	3 cut(s)	266, 489, 1521
Lsp1109I	GCAGC	9 cut(s)	179, 342, 479, 560, 1281, 1378, 1770, 1997, 2083
LweI	GCATC	5 cut(s)	798, 893, 1353, 1364, 1671
MaeI	CTAG	3 cut(s)	189, 464, 1155
MaeII	ACGT	3 cut(s)	546, 598, 891
MaeIII	GTNAC	8 cut(s)	475, 817, 956, 1020, 1244, 1462, 1916, 2109
MbiI	CCGCTC	3 cut(s)	261, 400, 2077
MfeI	CAATTG	1 cut(s)	471
MflI	RGATCY	2 cut(s)	1176, 1666
MhlI	GDGCHC	4 cut(s)	486, 510, 918, 1518
MluCI	AATT	7 cut(s)	471, 701, 750, 764, 1922, 1969, 2000
Mly113I	GGCGCC	1 cut(s)	49
MlyI	GAGTC	2 cut(s)	566, 1201
MmeI	TCCRAC	2 cut(s)	117, 156
Mph1103I	ATGCAT	1 cut(s)	1277
MroI	TCCGGA	1 cut(s)	1518
MroNI	GCCGGC	2 cut(s)	230, 1803
MseI	TTAA	8 cut(s)	723, 749, 1116, 1173, 1202, 1530, 1707, 1941
MslI	CAYNNNNRTG	2 cut(s)	161, 494
MspA1I	CMGCKG	2 cut(s)	1369, 1988
MspCI	CTTAAG	1 cut(s)	1940
MspI	CCGG	8 cut(s)	46, 231, 535, 1508, 1519, 1760, 1804, 2042
MspR9I	CCNGG	4 cut(s)	674, 1097, 1761, 1792
MunI	CAATTG	1 cut(s)	471
Mva1269I	GAATGC	2 cut(s)	149, 813
MvaI	CCWGG	3 cut(s)	674, 1097, 1792
MvnI	CGCG	6 cut(s)	339, 357, 359, 371, 373, 920
NaeI	GCCGGC	2 cut(s)	232, 1805
NarI	GGCGCC	1 cut(s)	49
NciI	CCSGG	1 cut(s)	1761
NdeI	CATATG	1 cut(s)	1271
NgoMIV	GCCGGC	2 cut(s)	230, 1803
NheI	GCTAGC	1 cut(s)	463
NlaIII	CATG	7 cut(s)	454, 495, 736, 1387, 1738, 1983, 2110
NmeAIII	GCCGAG	1 cut(s)	1701
NmuCI	GTSAC	6 cut(s)	475, 817, 956, 1244, 1462, 2109
NruI	TCGCGA	1 cut(s)	920
NsiI	ATGCAT	1 cut(s)	1277
NspI	RCATGY	1 cut(s)	495
NspV	TTCGAA	1 cut(s)	1967
OliI	CACNNNNGTG	2 cut(s)	161, 494
PagI	TCATGA	1 cut(s)	732
PaqCI	CACCTGC	1 cut(s)	1856
PceI	AGGCCT	3 cut(s)	224, 1624, 1722
PciSI	GCTCTTC	3 cut(s)	241, 407, 1146
PcsI	WCGNNNNNNNCGW	2 cut(s)	651, 1708
PctI	GAATGC	2 cut(s)	149, 813
PdiI	GCCGGC	2 cut(s)	232, 1805
PfeI	GAWTC	5 cut(s)	922, 1125, 1856, 2047, 2056
Pfl23II	CGTACG	1 cut(s)	543
PflMI	CCANNNNNTGG	2 cut(s)	846, 1194
PleI	GAGTC	2 cut(s)	565, 1201
PluTI	GGCGCC	1 cut(s)	52
PmaCI	CACGTG	1 cut(s)	599
PmlI	CACGTG	1 cut(s)	599
PpsI	GAGTC	2 cut(s)	565, 1201
Ppu21I	YACGTR	2 cut(s)	547, 599
PpuMI	RGGWCCY	1 cut(s)	169
Psp5II	RGGWCCY	1 cut(s)	169
Psp6I	CCWGG	3 cut(s)	672, 1095, 1790
PspCI	CACGTG	1 cut(s)	599
PspFI	CCCAGC	3 cut(s)	172, 295, 896
PspGI	CCWGG	3 cut(s)	672, 1095, 1790
PspLI	CGTACG	1 cut(s)	543
PspPI	GGNCC	4 cut(s)	33, 169, 311, 2039
PspPPI	RGGWCCY	1 cut(s)	169
PsuI	RGATCY	2 cut(s)	1176, 1666
PvuII	CAGCTG	2 cut(s)	1369, 1988
RruI	TCGCGA	1 cut(s)	920
RsaI	GTAC	5 cut(s)	318, 545, 1216, 1437, 1714
RsaNI	GTAC	5 cut(s)	317, 544, 1215, 1436, 1713
RseI	CAYNNNNRTG	2 cut(s)	161, 494
SalI	GTCGAC	1 cut(s)	392
SapI	GCTCTTC	3 cut(s)	241, 407, 1146
SaqAI	TTAA	8 cut(s)	723, 749, 1116, 1173, 1202, 1530, 1707, 1941
Sau96I	GGNCC	4 cut(s)	33, 169, 311, 2039
SchI	GAGTC	2 cut(s)	566, 1201
ScrFI	CCNGG	4 cut(s)	674, 1097, 1761, 1792
SduI	GDGCHC	4 cut(s)	486, 510, 918, 1518
SfaNI	GCATC	5 cut(s)	798, 893, 1353, 1364, 1671
SfcI	CTRYAG	2 cut(s)	527, 2017
SfoI	GGCGCC	1 cut(s)	50
SfuI	TTCGAA	1 cut(s)	1967
SinI	GGWCC	3 cut(s)	33, 169, 2039
SmiMI	CAYNNNNRTG	2 cut(s)	161, 494
SmlI	CTYRAG	1 cut(s)	1940
SmoI	CTYRAG	1 cut(s)	1940
Sse9I	AATT	7 cut(s)	471, 701, 750, 764, 1922, 1969, 2000
SseBI	AGGCCT	3 cut(s)	224, 1624, 1722
SspDI	GGCGCC	1 cut(s)	48
SspMI	CTAG	3 cut(s)	189, 464, 1155
StuI	AGGCCT	3 cut(s)	224, 1624, 1722
StyD4I	CCNGG	4 cut(s)	672, 1095, 1759, 1790
StyI	CCWWGG	2 cut(s)	26, 1717
TaaI	ACNGT	2 cut(s)	481, 531
TaiI	ACGT	3 cut(s)	549, 601, 894
TasI	AATT	7 cut(s)	471, 701, 750, 764, 1922, 1969, 2000
TatI	WGTACW	1 cut(s)	1214
TauI	GCSGC	2 cut(s)	203, 1087
TfiI	GAWTC	5 cut(s)	922, 1125, 1856, 2047, 2056
Tru1I	TTAA	8 cut(s)	723, 749, 1116, 1173, 1202, 1530, 1707, 1941
Tru9I	TTAA	8 cut(s)	723, 749, 1116, 1173, 1202, 1530, 1707, 1941
TscAI	CASTG	1 cut(s)	1253
TseFI	GTSAC	6 cut(s)	475, 817, 956, 1244, 1462, 2109
TseI	GCWGC	9 cut(s)	192, 330, 467, 573, 1294, 1366, 1783, 1985, 2096
Tsp45I	GTSAC	6 cut(s)	475, 817, 956, 1244, 1462, 2109
TspDTI	ATGAA	5 cut(s)	868, 1412, 1659, 1801, 2095
TspGWI	ACGGA	3 cut(s)	127, 1904, 1919
TspRI	CASTG	1 cut(s)	1253
Van91I	CCANNNNNTGG	2 cut(s)	846, 1194
Vha464I	CTTAAG	1 cut(s)	1940
VneI	GTGCAC	1 cut(s)	506
VpaK11BI	GGWCC	3 cut(s)	33, 169, 2039
XapI	RAATTY	1 cut(s)	1922
XceI	RCATGY	1 cut(s)	495
XmiI	GTMKAC	2 cut(s)	393, 780
XspI	CTAG	3 cut(s)	189, 464, 1155
Zsp2I	ATGCAT	1 cut(s)	1277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.