Rorug02G0524300

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003871
Physical Location & Seq
Reverse (-)
65468995 .. 65471680
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0524300.1

Sequence Viewer

Length: 1059 bp
ATGCCAATGGAGAGAGATCCGAGGAGAGGCAGCAATATCACTGCCTCAATTGCTAACCTTCCAGAATCATTGTTGGGCGATCAACCATCACCACCACCAGGAGGAGGAGGCAGGGCGTTTCCAACCATGCCTGGGTCGGAGTTTGCCCGATCCATTTACGCCGACCAGGCCAGAAGGGAGGTAGAGCAGTTGATTGAGGAGCTTAACGATCGTCGTACCCGTGAACAGGCTCTGCAAGGTCTCTCTAAGCTGAAGGAGGTTCCTGACGTGGCTCTATTACTGTGGCATACCAGTGGTCTCATGTTTATGCTCCTAAGGGAAATAACTGGTGTATACCACCTGATGTCAACCCCAAATCTTACTCAGGAAGTATCAAACCGAGTTTGCAATACCATAACTCTGTTTCAGTGTATAGCTTCTCATCTAGAGACAAGAAAGCCATTTATCAGAGCTAGCATGCCATTGTATCTCTACCCTTTCATTGGCACTGAGAACAAGGACAAGCCTCATGAATATCTCAGACTATCCAGCTTAGGTGCTCTTGGGGCCCTAGTGAAGGGTCACCCACCAAATAGAGATGAAGTAGATCCAGAAATTATACACTTCCTCCTAGAAAGCCAGGTATTTTGCCACTGCATCCGCTGCATGGAAGTTGGCGGTGTATTGTCAAGAACTGTGGCTACATTTATAGTTGAAAGAATTCTGACAACTGATGAGGGACTCAACTACTGCTGCAATTTCGGGGATCGGTTTTATGTAGTAACTCATGTTCTTGGAGAGATGACTGATAAGCTTCTTGAAGAACCTTCTGCCCGCCTGCTTAAACTTATCATAAAGTGCTTTCTTATACTGTCGAAAGGTCCAAGTCTGTGCAGGGACAGTGAAGGTTTAAGATGCTGTATTCCTAAGAGCTTAACACATCCCAATGCTATTAACCTCCTCTCTGGTGACCGAGACGCCATGCGGGGTCTGCAACAGCTCTTGAAAAATGTTTCAAATGGGCACCAGGTTCATGTATCACCCACTGGGGAGCTAGTTGGGTACTTGATCGATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

352

Amino Acids

39.23

Weight (kDa)

6.42

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rcd1 PF04078 64 - 331 4.5e-83 Cell differentiation family, Rcd1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1002
AccI GTMKAC 1 cut(s) 333
AciI CCGC 4 cut(s) 640, 657, 814, 964
AclWI GGATC 4 cut(s) 11, 144, 581, 753
AcsI RAATTY 1 cut(s) 699
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 957
AfaI GTAC 2 cut(s) 217, 1043
AfiI CCNNNNNNNGG 9 cut(s) 26, 98, 101, 104, 132, 226, 482, 556, 646
AgsI TTSAA 4 cut(s) 695, 800, 985, 996
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 5 cut(s) 97, 130, 165, 618, 1005
AluBI AGCT 9 cut(s) 202, 250, 416, 452, 531, 793, 912, 979, 1033
AluI AGCT 9 cut(s) 202, 250, 416, 452, 531, 793, 912, 979, 1033
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 4 cut(s) 245, 302, 422, 948
AlwI GGATC 4 cut(s) 11, 144, 581, 753
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 2 cut(s) 168, 546
ApaI GGGCCC 1 cut(s) 550
ApeKI GCWGC 3 cut(s) 30, 642, 732
ApoI RAATTY 1 cut(s) 699
Asp700I GAANNNNTTC 1 cut(s) 699
AspS9I GGNCC 3 cut(s) 546, 547, 860
AsuHPI GGTGA 4 cut(s) 81, 554, 959, 1011
AsuNHI GCTAGC 1 cut(s) 452
AvaII GGWCC 1 cut(s) 860
AxyI CCTNAGG 1 cut(s) 314
BaeGI GKGCMC 2 cut(s) 550, 1005
BanI GGYRCC 1 cut(s) 1002
BanII GRGCYC 1 cut(s) 550
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 3 cut(s) 42, 629, 719
BccI CCATC 2 cut(s) 94, 1046
BciT130I CCWGG 5 cut(s) 99, 132, 167, 620, 1007
BcoDI GTCTC 4 cut(s) 245, 302, 422, 948
BfaI CTAG 6 cut(s) 425, 453, 551, 611, 1034, 1057
BglI GCCNNNNNGGC 1 cut(s) 167
BisI GCNGC 3 cut(s) 31, 643, 733
BlsI GCNGC 3 cut(s) 32, 644, 734
Bme1390I CCNGG 5 cut(s) 99, 132, 167, 620, 1007
Bme18I GGWCC 1 cut(s) 860
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 546, 547, 860
BmiI GGNNCC 4 cut(s) 261, 547, 548, 1004
BmrFI CCNGG 5 cut(s) 99, 132, 167, 620, 1007
BmrI ACTGGG 1 cut(s) 1035
BmsI GCATC 2 cut(s) 645, 884
BmtI GCTAGC 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 1035
Bpu10I CCTNAGC 1 cut(s) 532
Bsa29I ATCGAT 1 cut(s) 1050
BsaHI GRCGYC 1 cut(s) 957
BsaI GGTCTC 2 cut(s) 245, 302
BsaJI CCNNGG 2 cut(s) 20, 131
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 9 cut(s) 26, 98, 101, 104, 132, 226, 482, 556, 646
Bse1I ACTGG 3 cut(s) 291, 331, 1030
Bse21I CCTNAGG 1 cut(s) 314
BseBI CCWGG 5 cut(s) 99, 132, 167, 620, 1007
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 2 cut(s) 20, 131
BseGI GGATG 2 cut(s) 636, 919
BseLI CCNNNNNNNGG 9 cut(s) 26, 98, 101, 104, 132, 226, 482, 556, 646
BseMII CTCAG 3 cut(s) 377, 480, 532
BseNI ACTGG 3 cut(s) 291, 331, 1030
BseRI GAGGAG 5 cut(s) 37, 117, 120, 212, 929
BseSI GKGCMC 2 cut(s) 550, 1005
BseXI GCAGC 3 cut(s) 42, 629, 719
BsgI GTGCAG 1 cut(s) 892
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 170, 548
BshNI GGYRCC 1 cut(s) 1002
BshVI ATCGAT 1 cut(s) 1050
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 541
BslFI GGGAC 2 cut(s) 732, 890
BslI CCNNNNNNNGG 9 cut(s) 26, 98, 101, 104, 132, 226, 482, 556, 646
BsmAI GTCTC 4 cut(s) 245, 302, 422, 948
BsmBI CGTCTC 1 cut(s) 948
BsmFI GGGAC 2 cut(s) 732, 890
BsnI GGCC 2 cut(s) 170, 548
Bso31I GGTCTC 2 cut(s) 245, 302
Bsp120I GGGCCC 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 541, 550, 1005
Bsp143I GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
BspACI CCGC 4 cut(s) 640, 657, 814, 964
BspANI GGCC 2 cut(s) 170, 548
BspCNI CTCAG 3 cut(s) 376, 481, 531
BspDI ATCGAT 1 cut(s) 1050
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 4 cut(s) 261, 547, 548, 1004
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 4 cut(s) 11, 144, 581, 753
BspT107I GGYRCC 1 cut(s) 1002
BspTNI GGTCTC 2 cut(s) 245, 302
BsrI ACTGG 3 cut(s) 291, 331, 1030
BssECI CCNNGG 2 cut(s) 20, 131
BssMI GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
BssNAI GTATAC 1 cut(s) 334
BssNI GRCGYC 1 cut(s) 957
Bst1107I GTATAC 1 cut(s) 334
Bst2UI CCWGG 5 cut(s) 99, 132, 167, 620, 1007
Bst4CI ACNGT 4 cut(s) 282, 676, 852, 881
BstACI GRCGYC 1 cut(s) 957
BstAPI GCANNNNNTGC 1 cut(s) 642
BstC8I GCNNGC 4 cut(s) 454, 458, 814, 818
BstDEI CTNAG 7 cut(s) 246, 314, 363, 489, 518, 532, 906
BstEII GGTNACC 2 cut(s) 560, 947
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 636, 919
BstKTI GATC 7 cut(s) 19, 82, 152, 211, 589, 748, 1050
BstMAI GTCTC 4 cut(s) 245, 302, 422, 948
BstMBI GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 5 cut(s) 50, 167, 545, 642, 970
BstNI CCWGG 5 cut(s) 99, 132, 167, 620, 1007
BstNSI RCATGY 1 cut(s) 460
BstPI GGTNACC 2 cut(s) 560, 947
BstSCI CCNGG 5 cut(s) 97, 130, 165, 618, 1005
BstSLI GKGCMC 2 cut(s) 550, 1005
BstV1I GCAGC 3 cut(s) 42, 629, 719
BstX2I RGATCY 2 cut(s) 16, 586
BstYI RGATCY 2 cut(s) 16, 586
BstZ17I GTATAC 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 1050
Bsu36I CCTNAGG 1 cut(s) 314
BsuRI GGCC 2 cut(s) 170, 548
BsuTUI ATCGAT 1 cut(s) 1050
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 2 cut(s) 636, 919
BtsI GCAGTG 2 cut(s) 39, 631
BtsIMutI CAGTG 7 cut(s) 39, 298, 413, 486, 631, 886, 1023
Cac8I GCNNGC 4 cut(s) 454, 458, 814, 818
CaiI CAGNNNCTG 1 cut(s) 232
CciI TCATGA 1 cut(s) 508
Cfr13I GGNCC 3 cut(s) 546, 547, 860
ClaI ATCGAT 1 cut(s) 1050
CseI GACGC 1 cut(s) 965
CsiI ACCWGGT 1 cut(s) 1005
Csp6I GTAC 2 cut(s) 216, 1042
CspCI CAANNNNNGTGG 2 cut(s) 657, 692
CviAII CATG 8 cut(s) 127, 301, 457, 509, 646, 767, 961, 1013
CviQI GTAC 2 cut(s) 216, 1042
DdeI CTNAG 7 cut(s) 246, 314, 363, 489, 518, 532, 906
DpnI GATC 7 cut(s) 18, 81, 151, 210, 588, 747, 1049
DpnII GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 2 cut(s) 245, 302
Eco47I GGWCC 1 cut(s) 860
Eco57I CTGAAG 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 314
Eco91I GGTNACC 2 cut(s) 560, 947
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 2 cut(s) 546, 547
EcoO65I GGTNACC 2 cut(s) 560, 947
EcoRI GAATTC 1 cut(s) 699
EcoRII CCWGG 5 cut(s) 97, 130, 165, 618, 1005
EcoT38I GRGCYC 1 cut(s) 550
Esp3I CGTCTC 1 cut(s) 948
FaeI CATG 8 cut(s) 130, 304, 460, 512, 649, 770, 964, 1016
FaqI GGGAC 2 cut(s) 732, 890
FatI CATG 8 cut(s) 126, 300, 456, 508, 645, 766, 960, 1012
FauI CCCGC 2 cut(s) 821, 957
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 31, 643, 733
FokI GGATG 2 cut(s) 623, 906
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 3 cut(s) 31, 643, 733
FspBI CTAG 6 cut(s) 425, 453, 551, 611, 1034, 1057
GluI GCNGC 3 cut(s) 31, 643, 733
HaeIII GGCC 2 cut(s) 170, 548
HgaI GACGC 1 cut(s) 965
Hin1I GRCGYC 1 cut(s) 957
Hin1II CATG 8 cut(s) 130, 304, 460, 512, 649, 770, 964, 1016
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 2 cut(s) 65, 720
HphI GGTGA 4 cut(s) 81, 554, 959, 1011
Hpy166II GTNNAC 3 cut(s) 224, 334, 348
Hpy188I TCNGA 5 cut(s) 21, 139, 449, 521, 705
Hpy188III TCNNGA 9 cut(s) 62, 263, 365, 425, 509, 590, 669, 797, 982
Hpy8I GTNNAC 3 cut(s) 224, 334, 348
Hpy99I CGWCG 1 cut(s) 216
HpyAV CCTTC 6 cut(s) 68, 168, 247, 550, 816, 878
HpyCH4III ACNGT 4 cut(s) 282, 676, 852, 881
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 7 cut(s) 235, 387, 636, 645, 735, 873, 973
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 167, 545, 642, 970
HpyF3I CTNAG 7 cut(s) 246, 314, 363, 489, 518, 532, 906
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 957
Hsp92II CATG 8 cut(s) 130, 304, 460, 512, 649, 770, 964, 1016
Kzo9I GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
LmnI GCTCC 3 cut(s) 199, 315, 1030
Lsp1109I GCAGC 3 cut(s) 42, 629, 719
LweI GCATC 2 cut(s) 645, 884
MabI ACCWGGT 1 cut(s) 1005
MaeI CTAG 6 cut(s) 425, 453, 551, 611, 1034, 1057
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 3 cut(s) 560, 760, 947
MalI GATC 7 cut(s) 18, 81, 151, 210, 588, 747, 1049
MboI GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
MboII GAAGA 1 cut(s) 812
MfeI CAATTG 1 cut(s) 48
MflI RGATCY 2 cut(s) 16, 586
MhlI GDGCHC 3 cut(s) 541, 550, 1005
MluCI AATT 4 cut(s) 48, 594, 699, 736
MlyI GAGTC 1 cut(s) 714
MmeI TCCRAC 2 cut(s) 117, 146
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 5 cut(s) 204, 822, 890, 914, 933
MslI CAYNNNNRTG 3 cut(s) 291, 305, 924
MspA1I CMGCKG 1 cut(s) 642
MspR9I CCNGG 5 cut(s) 99, 132, 167, 620, 1007
MunI CAATTG 1 cut(s) 48
MvaI CCWGG 5 cut(s) 99, 132, 167, 620, 1007
MwoI GCNNNNNNNGC 5 cut(s) 50, 167, 545, 642, 970
NdeII GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 8 cut(s) 130, 304, 460, 512, 649, 770, 964, 1016
NlaIV GGNNCC 4 cut(s) 261, 547, 548, 1004
NmuCI GTSAC 2 cut(s) 560, 947
NspI RCATGY 1 cut(s) 460
PaeI GCATGC 1 cut(s) 460
PagI TCATGA 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 3 cut(s) 32, 644, 734
Ple19I CGATCG 1 cut(s) 211
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
Psp6I CCWGG 5 cut(s) 97, 130, 165, 618, 1005
PspEI GGTNACC 2 cut(s) 560, 947
PspGI CCWGG 5 cut(s) 97, 130, 165, 618, 1005
PspN4I GGNNCC 4 cut(s) 261, 547, 548, 1004
PspOMI GGGCCC 1 cut(s) 546
PspPI GGNCC 3 cut(s) 546, 547, 860
PsrI GAACNNNNNNTAC 4 cut(s) 664, 696, 753, 785
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 16, 586
PvuI CGATCG 1 cut(s) 211
RsaI GTAC 2 cut(s) 217, 1043
RsaNI GTAC 2 cut(s) 216, 1042
RseI CAYNNNNRTG 3 cut(s) 291, 305, 924
SaqAI TTAA 5 cut(s) 204, 822, 890, 914, 933
SatI GCNGC 3 cut(s) 31, 643, 733
Sau3AI GATC 7 cut(s) 16, 79, 149, 208, 586, 745, 1047
Sau96I GGNCC 3 cut(s) 546, 547, 860
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 5 cut(s) 99, 132, 167, 620, 1007
SduI GDGCHC 3 cut(s) 541, 550, 1005
SexAI ACCWGGT 1 cut(s) 1005
SfaNI GCATC 2 cut(s) 645, 884
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 3 cut(s) 291, 305, 924
SphI GCATGC 1 cut(s) 460
Sse9I AATT 4 cut(s) 48, 594, 699, 736
SsiI CCGC 4 cut(s) 640, 657, 814, 964
SspMI CTAG 6 cut(s) 425, 453, 551, 611, 1034, 1057
StyD4I CCNGG 5 cut(s) 97, 130, 165, 618, 1005
TaaI ACNGT 4 cut(s) 282, 676, 852, 881
TaiI ACGT 1 cut(s) 270
TaqI TCGA 2 cut(s) 854, 1050
TaqII GACCGA 1 cut(s) 966
TasI AATT 4 cut(s) 48, 594, 699, 736
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 5 cut(s) 204, 822, 890, 914, 933
Tru9I TTAA 5 cut(s) 204, 822, 890, 914, 933
TscAI CASTG 7 cut(s) 46, 298, 413, 493, 638, 886, 1030
TseFI GTSAC 2 cut(s) 560, 947
TseI GCWGC 3 cut(s) 30, 642, 732
Tsp45I GTSAC 2 cut(s) 560, 947
TspDTI ATGAA 4 cut(s) 469, 525, 594, 1001
TspRI CASTG 7 cut(s) 46, 298, 413, 493, 638, 886, 1030
VpaK11BI GGWCC 1 cut(s) 860
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 699
XbaI TCTAGA 1 cut(s) 424
XceI RCATGY 1 cut(s) 460
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 6 cut(s) 425, 453, 551, 611, 1034, 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.