Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004546

Physical Location & Seq

Genomic Coordinates Reverse (-)

4665071 .. 4669812

Gene Structure 4742 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1068400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3162 bp

ATGTCTGCAAAGATTTTGCATTCTCTAACGGATGAGAACCGGGACCTCCATAAGCAAATTGGGTGCATGAGTGGAATTTTTCAGCTCTTTGATCGCCACCATTTCCTCTCCGGCCGGCGTATCAACGGCCAGAACCACAAGAGACTTCCTCCAGGTGAGAATTGCAACCACGCAGTCGAACCAAGCACAGTGCAGAAAGCTACAGAAAAAATTCGAAAAAAGGCGGTGAAAGAGAAACAACGAAACTCCACAGAATCATCGAAAAGCACTGTTTCTTCTTCTTCTTCGTGTTCGTCTAGCTTGTCGTCTTTTGAACATAAAAAGGCAACTCAGCAAGAACCATCTTCGCCCAGCCAAACCATTTCCAATGACAAAGACGCTCGGGACCCATTCGTGAAACAACCAAATGTTTTGATGCACATACGTCGGCAATCCTTTGATCTGCAAGGTCTTGTCAAAGACTCGATGCACAGAGAAGCCCGTGGCATTTCAGTTAAACCTGCAGCTAAAGACGGAGTTGGTCACACATTGAAGTACATAGACTCCCCGAGGCCTTCAATAAAATCCACCAAGCCAAGGGTTTCTGGTGGTAACGAGTCATTTCAAGTTCCCGCAAAGCTTCAGGTAGCAACTTGGAGTTCCAATGAAGAGAAGGATGGTTGCGCGCGCTTCTTACCAAAAGATGCTCGGAGGTTCTCTTATGATGGAAGAGAGTCGCGAGATGCATCAAAATCCACCATAAAACTCAAAGAACTCCCAAGGCTTTCTTTGGACAGTAAGGAACGTTCCATAAGGAGGGGTTGTAACCCAGAAACAAAAGGAAACTACTCCATCAAGGATCTGCAGAGAGAGGACAGGAACTGCAACAAATTGCTTGACTTGCAGCATGAACCCGGAAGCGCTAAAAGACCGTCTAATGTCGTTGCAAAGTTGATGGGGTTGGATCTACCAGATTCCGTGTCAACCACTGATAGTCCATTAAGGCTGATTAATACTGACCAATCTGACAGATCTGACCCCTTCTCCAGATCATCAAGAGCAACCAATGAAAACAAGCAAGATACTTTTTCTGGGATACTCCTTGCAAATACAAAGAAAGACAGCTCACCGCAGAGGAGAGGCACTGATCCGGTCATGAAGCCCACCGCCAATTCTAAGTTTCCAATAGAAGCAGCTCCGTGGAGGCAGCCACACAGAAGCAAGGGTTCTCAACGATCAGATTTCAAGCAACAAGAAGAACCTATAAAAGCTCCAAATTCATCATCTTCAGTATATGGTGAAATGGAAAAAAGGCTGGCAGATCTTGAGTTCAAAAAATCAGGAAAGGATCTCAGAGCTCTTAAGCAAATACTTGAAGTGATGCAGAAGACGAAAGGAATGATAGATACAAGAAAGGATCACGCCTCAAATTTTGCATCTCAAATAAGCAACGAAAGCATTTCTTTGGACAGCACGGTATCAGCTAGCCAGAGATATCTACAAAGTAACATATCAGTTCCTGCCACGGCCAAGGGTTCCCAATCTCCAAAGAGTTATAAATCACCCACCGTCATACTGAAACCAGCTAAACTCATTGATAAAACCCACAGCTCTGCTTCCATGGTGAATTCCATGAATGACACATTGGGTCTTCGGAAGCTTCGGACCAGTGACCCTGGTGATAACAGAAAGGGATTGGTCGACAAGAAAACAGCCAAAGATCTGACACCAAGGAACAATCATGTCAGAGATCCCTTCAATCGACGACTCCATTCCACTGATGATAATTCTAACACAAGAACTTTGAAATCTGCACAAAAACCAAAGGCATCGCAAACTATGAGTGAAGAAAAAGTCACCAGCTCAAGTAGGAGTCCAGGAATCAGGAGCCCAAAAATGCAACAGAGAAGACTTGGATTGGAGAAGCAATCTCCTCCAAGCACTCCATCATCGAATTCAAGCATGACGAGAAGGCAACACACCAAGCAGTCACTAGAAGCAACCACCCCAGGTAGAAAACTTGGACACAAGACACCCAGTTTGCATCAAAGTAATGTGCAACTGAGGGAGACTAGCACCAAGACGAGAGATATGAGCCATCACGATGATTCCACTTCTCAGCAATCTGGGAGCACCATCAGCTTGGCCTTGCATACAGACACAGAAGCCACAAGCATTCATCAATCAGATACAATCACAGGCACGCATTTCAACAAGCATAACCAAAAAATAAAGCACCCAGCAGTGGGGTTGATTGATGACAGGTCAATGGCAGAAACTAGGAAAGCTTCCTCCGAACAACCAAGCCCTGTCTCTGTTCTTGATTCCACATTCTATCGCGATGACTCACCGTCTCCAGTGAAGAAGATATCAAATGCCTTCAAAGACGATGAAGCACAAATTCTGGAGATAGCAGAGTATGACCCAATGAACCAAGCTCTGTTATACAACAACACAATGCCCAGTCTTGGGGCAGAAATTGATCATAAAAGATTAGAGAACCTGATTCAGAATCATCGATGCATGAGCAGGACACATGAGGACCCCATCACTGGTCCCCTCTGTGACAGTACAAATCCAGACCACATGTACATTTCAGATATACTGCAGGCATCAGGTATTCTCGGGTACCTCAAATCTGCCTGGACAACCAGTGAGCTCAACACATCAGACCACCTGATCAACCCTAATTTGTTCCTTGCACTGGAAGATATCAGAACAAATACAATGCCTTCTGATCTCCAGCCCAAACCTGATGAGAAAATTCAAAGAAAACAAGTATTTGAGGTTGTTAATGAATTCCTTGTTCAAAAACTAGTGGTTGGAGAATCTTTGAAGCAGTGGTTCCCACCAAACAAGCTGGCAGATGGGAAACCAAGAAGCCAGCAGCTTCTGAAAGAATTGTGTTCAGAGGTTGATCAGCTACGAAGAATGAACTTGAATGGCAGCCTGGATGACGAGGATGAAAGTTTGAGAAACATCTTGTTGGAAGATTTCACGGATCAAGCAAAGAATTGGACAGAGTGTGACAGTGAAATTCCTTCTGTAGTGTTGGATGTCGAGCGGTTGATCTTTAAAGATTTGATAAATGAAATTGTGAGTGATGATTCAGTTGGCCTGCATGGTTGGTCCGGTGGGCATTGCAGGCAACTGTTTTCTGGGAAGGGGCTAAGGAAATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0008017 GO:0008092 GO:0008150 GO:0015631 GO:0022603 GO:0022604 GO:0040008 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051510 GO:0051513 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

1054

Amino Acids

117.79

Weight (kDa)

9.39

Isoelectric Point (pI)

61.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4378	PF14309	854 - 1024	4.4e-28	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004546)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46710 FvH4_6g46710 FvH4_6g46710 FvH4_6g46710
malus_domestica	MD09G1068400.v1.1 MD17G1061300.v1.1
prunus_persica	Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1
pyrus_communis	pycom12433g00110 pycom12433g00120 pycom17g06120
rosa_chinensis	RchiOBHm_Chr2g0165601
rosa_laevigata	RLG00000021542
rosa_multiflora	Rmu_co8268985.1_g000001 Rmu_co8395029.1_g000001 Rmu_sc0005425.1_g000021 Rmu_sc0035818.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085700
rosa_rugosa	Rorug02G0516300 Rorug02G0516400 Rorug02G0516400 Rorug02G0516400
rosa_samantha	Rh2AG582500 Rh2BG594600 Rh2CG564500 Rh2DG604500
rosa_wichuraiana	Rw0G016060 Rw2G048590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1536
Acc36I	ACCTGC	1 cut(s)	508
Acc65I	GGTACC	1 cut(s)	2607
AccB1I	GGYRCC	1 cut(s)	2607
AccBSI	CCGCTC	1 cut(s)	3043
AccI	GTMKAC	1 cut(s)	1680
AccII	CGCG	4 cut(s)	665, 667, 718, 2319
AciI	CCGC	5 cut(s)	224, 612, 1109, 1146, 3043
AclI	AACGTT	1 cut(s)	784
AclWI	GGATC	7 cut(s)	846, 951, 1121, 1335, 1404, 1724, 2988
AcoI	YGGCCR	3 cut(s)	112, 127, 1506
AcuI	CTGAAG	2 cut(s)	605, 1251
AfaI	GTAC	4 cut(s)	536, 2551, 2570, 2609
AfeI	AGCGCT	1 cut(s)	901
AfiI	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
AflII	CTTAAG	1 cut(s)	1340
AflIII	ACRYGT	1 cut(s)	2565
AhdI	GACNNNNNGTC	1 cut(s)	2329
AhlI	ACTAGT	1 cut(s)	2794
AjnI	CCWGG	6 cut(s)	151, 1654, 1855, 1987, 2621, 2928
AjuI	GAANNNNNNNTTGG	2 cut(s)	1607, 1639
Alw21I	GWGCWC	3 cut(s)	1339, 2114, 2640
Alw26I	GTCTC	4 cut(s)	136, 2042, 2296, 2337
AlwI	GGATC	7 cut(s)	846, 951, 1121, 1335, 1404, 1724, 2988
AlwNI	CAGNNNCTG	3 cut(s)	861, 1499, 2872
Ama87I	CYCGRG	3 cut(s)	381, 547, 2603
Aor51HI	AGCGCT	1 cut(s)	901
AoxI	GGCC	6 cut(s)	112, 127, 551, 1506, 2124, 3094
ApeKI	GCWGC	6 cut(s)	503, 883, 1172, 1186, 2866, 2925
ArsI	GACNNNNNNTTYG	2 cut(s)	2002, 2034
AseI	ATTAAT	1 cut(s)	990
Asp700I	GAANNNNTTC	1 cut(s)	210
Asp718I	GGTACC	1 cut(s)	2607
AspLEI	GCGC	4 cut(s)	665, 667, 669, 902
AspS9I	GGNCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
AsuC2I	CCSGG	2 cut(s)	41, 894
AsuHPI	GGTGA	9 cut(s)	167, 238, 1098, 1289, 1533, 1615, 1670, 1828, 2319
AsuII	TTCGAA	1 cut(s)	214
AsuNHI	GCTAGC	1 cut(s)	1463
AvaI	CYCGRG	3 cut(s)	381, 547, 2603
AvaII	GGWCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
BanI	GGYRCC	1 cut(s)	2607
BanII	GRGCYC	3 cut(s)	1339, 1871, 2640
BbsI	GAAGAC	3 cut(s)	1373, 1622, 1894
Bbv12I	GWGCWC	3 cut(s)	1339, 2114, 2640
BbvI	GCAGC	6 cut(s)	515, 895, 1184, 1198, 2878, 2937
BceAI	ACGGC	2 cut(s)	142, 1521
BcgI	CGANNNNNNTGC	2 cut(s)	407, 441
BciT130I	CCWGG	6 cut(s)	153, 1656, 1857, 1989, 2623, 2930
BciVI	GTATCC	1 cut(s)	1068
BclI	TGATCA	3 cut(s)	2461, 2658, 2896
BcnI	CCSGG	2 cut(s)	41, 894
BcoDI	GTCTC	4 cut(s)	136, 2042, 2296, 2337
BcuI	ACTAGT	1 cut(s)	2794
BfaI	CTAG	6 cut(s)	297, 1464, 1973, 2052, 2259, 2795
BfmI	CTRYAG	5 cut(s)	201, 501, 842, 2585, 3024
BfoI	RGCGCY	1 cut(s)	903
BfrI	CTTAAG	1 cut(s)	1340
BfuAI	ACCTGC	1 cut(s)	508
BfuI	GTATCC	1 cut(s)	1068
BglII	AGATCT	3 cut(s)	1010, 1300, 1699
BisI	GCNGC	6 cut(s)	504, 884, 1173, 1187, 2867, 2926
BlsI	GCNGC	6 cut(s)	505, 885, 1174, 1188, 2868, 2927
Bme1390I	CCNGG	8 cut(s)	41, 153, 894, 1656, 1857, 1989, 2623, 2930
Bme18I	GGWCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
BmeRI	GACNNNNNGTC	1 cut(s)	2329
BmeT110I	CYCGRG	3 cut(s)	381, 547, 2603
BmgT120I	GGNCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
BmiI	GGNNCC	9 cut(s)	44, 386, 387, 1516, 1868, 2523, 2536, 2609, 2825
BmrFI	CCNGG	8 cut(s)	41, 153, 894, 1656, 1857, 1989, 2623, 2930
BmrI	ACTGGG	2 cut(s)	2010, 2436
BmtI	GCTAGC	1 cut(s)	1467
BmuI	ACTGGG	2 cut(s)	2010, 2436
BpiI	GAAGAC	3 cut(s)	1373, 1622, 1894
BplI	GAGNNNNNCTC	2 cut(s)	133, 165
BpmI	CTGGAG	5 cut(s)	135, 1009, 2319, 2405, 2705
Bpu10I	CCTNAGC	1 cut(s)	3149
Bpu14I	TTCGAA	1 cut(s)	214
BpuEI	CTTGAG	2 cut(s)	1325, 1828
BpuMI	CCSGG	2 cut(s)	41, 894
Bsa29I	ATCGAT	1 cut(s)	2497
BsaWI	WCCGGW	2 cut(s)	1129, 3110
BsaXI	ACNNNNNCTCC	6 cut(s)	527, 557, 787, 817, 1007, 1037
Bsc4I	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
Bse118I	RCCGGY	1 cut(s)	114
Bse1I	ACTGG	7 cut(s)	1647, 2016, 2336, 2442, 2536, 2631, 2688
Bse3DI	GCAATG	1 cut(s)	3118
BseBI	CCWGG	6 cut(s)	153, 1656, 1857, 1989, 2623, 2930
BseCI	ATCGAT	1 cut(s)	2497
BseGI	GGATG	5 cut(s)	37, 661, 2938, 2947, 3040
BseLI	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
BseMI	GCAATG	1 cut(s)	3118
BseMII	CTCAG	4 cut(s)	344, 1345, 2033, 2111
BseNI	ACTGG	7 cut(s)	1647, 2016, 2336, 2442, 2536, 2631, 2688
BsePI	GCGCGC	2 cut(s)	663, 665
BseRI	GAGGAG	2 cut(s)	1129, 1902
BseX3I	CGGCCG	1 cut(s)	112
BseXI	GCAGC	6 cut(s)	515, 895, 1184, 1198, 2878, 2937
BseYI	CCCAGC	2 cut(s)	350, 2218
BsgI	GTGCAG	2 cut(s)	212, 1776
Bsh1236I	CGCG	4 cut(s)	665, 667, 718, 2319
Bsh1285I	CGRYCG	1 cut(s)	115
BshFI	GGCC	6 cut(s)	114, 129, 553, 1508, 2126, 3096
BshNI	GGYRCC	1 cut(s)	2607
BshVI	ATCGAT	1 cut(s)	2497
BsiEI	CGRYCG	1 cut(s)	115
BsiHKAI	GWGCWC	3 cut(s)	1339, 2114, 2640
BsiHKCI	CYCGRG	3 cut(s)	381, 547, 2603
BsiSI	CCGG	6 cut(s)	40, 111, 115, 894, 1130, 3111
BslFI	GGGAC	3 cut(s)	56, 398, 2520
BslI	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
BsmAI	GTCTC	4 cut(s)	136, 2042, 2296, 2337
BsmBI	CGTCTC	1 cut(s)	2337
BsmFI	GGGAC	3 cut(s)	56, 398, 2520
BsmI	GAATGC	2 cut(s)	19, 2154
BsnI	GGCC	6 cut(s)	114, 129, 553, 1508, 2126, 3096
BsoBI	CYCGRG	3 cut(s)	381, 547, 2603
Bsp119I	TTCGAA	1 cut(s)	214
Bsp1286I	GDGCHC	4 cut(s)	1339, 1871, 2114, 2640
Bsp1407I	TGTACA	1 cut(s)	2568
Bsp19I	CCATGG	1 cut(s)	1599
Bsp68I	TCGCGA	2 cut(s)	718, 2319
BspACI	CCGC	5 cut(s)	224, 612, 1109, 1146, 3043
BspANI	GGCC	6 cut(s)	114, 129, 553, 1508, 2126, 3096
BspCNI	CTCAG	4 cut(s)	343, 1344, 2034, 2110
BspDI	ATCGAT	1 cut(s)	2497
BspFNI	CGCG	4 cut(s)	665, 667, 718, 2319
BspHI	TCATGA	1 cut(s)	1134
BspLI	GGNNCC	9 cut(s)	44, 386, 387, 1516, 1868, 2523, 2536, 2609, 2825
BspMAI	CTGCAG	3 cut(s)	505, 846, 2589
BspMI	ACCTGC	1 cut(s)	508
BspOI	GCTAGC	1 cut(s)	1467
BspPI	GGATC	7 cut(s)	846, 951, 1121, 1335, 1404, 1724, 2988
BspT104I	TTCGAA	1 cut(s)	214
BspT107I	GGYRCC	1 cut(s)	2607
BspTI	CTTAAG	1 cut(s)	1340
BsrBI	CCGCTC	1 cut(s)	3043
BsrDI	GCAATG	1 cut(s)	3118
BsrFI	RCCGGY	1 cut(s)	114
BsrGI	TGTACA	1 cut(s)	2568
BsrI	ACTGG	7 cut(s)	1647, 2016, 2336, 2442, 2536, 2631, 2688
BssAI	RCCGGY	1 cut(s)	114
BssHII	GCGCGC	2 cut(s)	663, 665
BssT1I	CCWWGG	5 cut(s)	575, 758, 1509, 1599, 1709
Bst2UI	CCWGG	6 cut(s)	153, 1656, 1857, 1989, 2623, 2930
Bst6I	CTCTTC	2 cut(s)	642, 703
BstAFI	CTTAAG	1 cut(s)	1340
BstAUI	TGTACA	1 cut(s)	2568
BstBI	TTCGAA	1 cut(s)	214
BstDEI	CTNAG	6 cut(s)	330, 1155, 1331, 2042, 2097, 3149
BstDSI	CCRYGG	4 cut(s)	481, 1178, 1503, 1599
BstF5I	GGATG	5 cut(s)	37, 661, 2938, 2947, 3040
BstFNI	CGCG	4 cut(s)	665, 667, 718, 2319
BstH2I	RGCGCY	1 cut(s)	903
BstHHI	GCGC	4 cut(s)	665, 667, 669, 902
BstMAI	GTCTC	4 cut(s)	136, 2042, 2296, 2337
BstMCI	CGRYCG	1 cut(s)	115
BstMWI	GCNNNNNNNGC	4 cut(s)	880, 1434, 2118, 3124
BstNI	CCWGG	6 cut(s)	153, 1656, 1857, 1989, 2623, 2930
BstNSI	RCATGY	1 cut(s)	2569
BstSCI	CCNGG	8 cut(s)	39, 151, 892, 1654, 1855, 1987, 2621, 2928
BstSFI	CTRYAG	5 cut(s)	201, 501, 842, 2585, 3024
BstUI	CGCG	4 cut(s)	665, 667, 718, 2319
BstV1I	GCAGC	6 cut(s)	515, 895, 1184, 1198, 2878, 2937
BstV2I	GAAGAC	3 cut(s)	1373, 1622, 1894
BstX2I	RGATCY	7 cut(s)	838, 943, 1010, 1300, 1327, 1699, 1729
BstXI	CCANNNNNNTGG	1 cut(s)	2122
BstYI	RGATCY	7 cut(s)	838, 943, 1010, 1300, 1327, 1699, 1729
BstZI	CGGCCG	1 cut(s)	112
Bsu15I	ATCGAT	1 cut(s)	2497
BsuI	GTATCC	1 cut(s)	1068
BsuRI	GGCC	6 cut(s)	114, 129, 553, 1508, 2126, 3096
BsuTUI	ATCGAT	1 cut(s)	2497
BtgI	CCRYGG	4 cut(s)	481, 1178, 1503, 1599
BtgZI	GCGATG	2 cut(s)	1794, 2334
BtsCI	GGATG	5 cut(s)	37, 661, 2938, 2947, 3040
BtsI	GCAGTG	2 cut(s)	2229, 2825
BtuMI	TCGCGA	2 cut(s)	718, 2319
BveI	ACCTGC	1 cut(s)	508
CaiI	CAGNNNCTG	3 cut(s)	861, 1499, 2872
CciI	TCATGA	1 cut(s)	1134
CfoI	GCGC	4 cut(s)	665, 667, 669, 902
Cfr10I	RCCGGY	1 cut(s)	114
Cfr13I	GGNCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
ClaI	ATCGAT	1 cut(s)	2497
CseI	GACGC	1 cut(s)	386
Csp6I	GTAC	4 cut(s)	535, 2550, 2569, 2608
CviQI	GTAC	4 cut(s)	535, 2550, 2569, 2608
DdeI	CTNAG	6 cut(s)	330, 1155, 1331, 2042, 2097, 3149
DraI	TTTAAA	1 cut(s)	3055
DriI	GACNNNNNGTC	1 cut(s)	2329
EaeI	YGGCCR	3 cut(s)	112, 127, 1506
EagI	CGGCCG	1 cut(s)	112
Eam1104I	CTCTTC	2 cut(s)	642, 703
Eam1105I	GACNNNNNGTC	1 cut(s)	2329
EarI	CTCTTC	2 cut(s)	642, 703
Ecl136II	GAGCTC	2 cut(s)	1337, 2638
EclXI	CGGCCG	1 cut(s)	112
Eco130I	CCWWGG	5 cut(s)	575, 758, 1509, 1599, 1709
Eco147I	AGGCCT	1 cut(s)	553
Eco24I	GRGCYC	3 cut(s)	1339, 1871, 2640
Eco32I	GATATC	3 cut(s)	1475, 2349, 2692
Eco47I	GGWCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
Eco47III	AGCGCT	1 cut(s)	901
Eco52I	CGGCCG	1 cut(s)	112
Eco53kI	GAGCTC	2 cut(s)	1337, 2638
Eco57I	CTGAAG	2 cut(s)	605, 1251
Eco88I	CYCGRG	3 cut(s)	381, 547, 2603
EcoICRI	GAGCTC	2 cut(s)	1337, 2638
EcoO109I	RGGNCCY	3 cut(s)	43, 385, 2521
EcoRI	GAATTC	3 cut(s)	1606, 1933, 2777
EcoRII	CCWGG	6 cut(s)	151, 1654, 1855, 1987, 2621, 2928
EcoRV	GATATC	3 cut(s)	1475, 2349, 2692
EcoT14I	CCWWGG	5 cut(s)	575, 758, 1509, 1599, 1709
EcoT22I	ATGCAT	2 cut(s)	727, 2504
EcoT38I	GRGCYC	3 cut(s)	1339, 1871, 2640
ErhI	CCWWGG	5 cut(s)	575, 758, 1509, 1599, 1709
Esp3I	CGTCTC	1 cut(s)	2337
FalI	AAGNNNNNCTT	4 cut(s)	751, 783, 1426, 1458
FaqI	GGGAC	3 cut(s)	56, 398, 2520
FauI	CCCGC	1 cut(s)	619
FbaI	TGATCA	3 cut(s)	2461, 2658, 2896
FblI	GTMKAC	1 cut(s)	1680
Fnu4HI	GCNGC	6 cut(s)	504, 884, 1173, 1187, 2867, 2926
FokI	GGATG	5 cut(s)	44, 668, 2945, 2954, 3047
FriOI	GRGCYC	3 cut(s)	1339, 1871, 2640
Fsp4HI	GCNGC	6 cut(s)	504, 884, 1173, 1187, 2867, 2926
FspBI	CTAG	6 cut(s)	297, 1464, 1973, 2052, 2259, 2795
GlaI	GCGC	4 cut(s)	664, 666, 668, 901
GluI	GCNGC	6 cut(s)	504, 884, 1173, 1187, 2867, 2926
GsaI	CCCAGC	2 cut(s)	354, 2222
GsuI	CTGGAG	5 cut(s)	135, 1009, 2319, 2405, 2705
HaeII	RGCGCY	1 cut(s)	903
HaeIII	GGCC	6 cut(s)	114, 129, 553, 1508, 2126, 3096
HapII	CCGG	6 cut(s)	40, 111, 115, 894, 1130, 3111
HgaI	GACGC	1 cut(s)	386
HhaI	GCGC	4 cut(s)	665, 667, 669, 902
Hin6I	GCGC	4 cut(s)	663, 665, 667, 900
HinP1I	GCGC	4 cut(s)	663, 665, 667, 900
HincII	GTYRAC	2 cut(s)	963, 1681
HindII	GTYRAC	2 cut(s)	963, 1681
HindIII	AAGCTT	3 cut(s)	617, 1637, 2265
HpaII	CCGG	6 cut(s)	40, 111, 115, 894, 1130, 3111
HphI	GGTGA	9 cut(s)	167, 238, 1098, 1289, 1533, 1615, 1670, 1828, 2319
Hpy166II	GTNNAC	2 cut(s)	963, 1681
Hpy8I	GTNNAC	2 cut(s)	963, 1681
Hpy99I	CGWCG	2 cut(s)	429, 1746
HpyAV	CCTTC	9 cut(s)	564, 646, 1030, 1744, 1944, 2368, 2721, 3030, 3136
HpyCH4IV	ACGT	2 cut(s)	424, 784
HpyF10VI	GCNNNNNNNGC	4 cut(s)	880, 1434, 2118, 3124
HpyF3I	CTNAG	6 cut(s)	330, 1155, 1331, 2042, 2097, 3149
HpySE526I	ACGT	2 cut(s)	424, 784
HspAI	GCGC	4 cut(s)	663, 665, 667, 900
KflI	GGGWCCC	1 cut(s)	385
KpnI	GGTACC	1 cut(s)	2611
KroI	GCCGGC	1 cut(s)	114
KroNI	GCCGGC	1 cut(s)	116
Ksp22I	TGATCA	3 cut(s)	2461, 2658, 2896
LmnI	GCTCC	4 cut(s)	1180, 1255, 1866, 2109
Lsp1109I	GCAGC	6 cut(s)	515, 895, 1184, 1198, 2878, 2937
MaeI	CTAG	6 cut(s)	297, 1464, 1973, 2052, 2259, 2795
MaeII	ACGT	2 cut(s)	424, 784
MaeIII	GTNAC	9 cut(s)	521, 590, 803, 1484, 1649, 1834, 1968, 2543, 3005
MbiI	CCGCTC	1 cut(s)	3043
MflI	RGATCY	7 cut(s)	838, 943, 1010, 1300, 1327, 1699, 1729
MhlI	GDGCHC	4 cut(s)	1339, 1871, 2114, 2640
MlyI	GAGTC	7 cut(s)	455, 536, 605, 722, 1740, 1861, 2318
MmeI	TCCRAC	4 cut(s)	921, 2782, 2946, 3012
Mph1103I	ATGCAT	2 cut(s)	727, 2504
MroNI	GCCGGC	1 cut(s)	114
MroXI	GAANNNNTTC	1 cut(s)	210
MseI	TTAA	6 cut(s)	495, 980, 990, 1341, 2772, 3054
MslI	CAYNNNNRTG	1 cut(s)	2082
MspCI	CTTAAG	1 cut(s)	1340
MspI	CCGG	6 cut(s)	40, 111, 115, 894, 1130, 3111
MspR9I	CCNGG	8 cut(s)	41, 153, 894, 1656, 1857, 1989, 2623, 2930
Mva1269I	GAATGC	2 cut(s)	19, 2154
MvaI	CCWGG	6 cut(s)	153, 1656, 1857, 1989, 2623, 2930
MvnI	CGCG	4 cut(s)	665, 667, 718, 2319
MwoI	GCNNNNNNNGC	4 cut(s)	880, 1434, 2118, 3124
NaeI	GCCGGC	1 cut(s)	116
NciI	CCSGG	2 cut(s)	41, 894
NcoI	CCATGG	1 cut(s)	1599
NgoMIV	GCCGGC	1 cut(s)	114
NheI	GCTAGC	1 cut(s)	1463
NlaIV	GGNNCC	9 cut(s)	44, 386, 387, 1516, 1868, 2523, 2536, 2609, 2825
NmuCI	GTSAC	6 cut(s)	521, 1649, 1834, 1968, 2543, 3005
NruI	TCGCGA	2 cut(s)	718, 2319
NsiI	ATGCAT	2 cut(s)	727, 2504
NspI	RCATGY	1 cut(s)	2569
NspV	TTCGAA	1 cut(s)	214
PagI	TCATGA	1 cut(s)	1134
PauI	GCGCGC	2 cut(s)	663, 665
PceI	AGGCCT	1 cut(s)	553
PciI	ACATGT	1 cut(s)	2565
PctI	GAATGC	2 cut(s)	19, 2154
PdiI	GCCGGC	1 cut(s)	116
PdmI	GAANNNNTTC	1 cut(s)	210
PfeI	GAWTC	9 cut(s)	254, 953, 1860, 2087, 2303, 2485, 2491, 2807, 3086
PfoI	TCCNGGA	1 cut(s)	1855
PkrI	GCNGC	6 cut(s)	505, 885, 1174, 1188, 2868, 2927
PleI	GAGTC	7 cut(s)	455, 536, 604, 721, 1740, 1860, 2318
PpsI	GAGTC	7 cut(s)	455, 536, 604, 721, 1740, 1860, 2318
PpuMI	RGGWCCY	3 cut(s)	43, 385, 2521
PscI	ACATGT	1 cut(s)	2565
PshBI	ATTAAT	1 cut(s)	990
PsiI	TTATAA	1 cut(s)	1536
Psp124BI	GAGCTC	2 cut(s)	1339, 2640
Psp1406I	AACGTT	1 cut(s)	784
Psp5II	RGGWCCY	3 cut(s)	43, 385, 2521
Psp6I	CCWGG	6 cut(s)	151, 1654, 1855, 1987, 2621, 2928
PspFI	CCCAGC	2 cut(s)	350, 2218
PspGI	CCWGG	6 cut(s)	151, 1654, 1855, 1987, 2621, 2928
PspN4I	GGNNCC	9 cut(s)	44, 386, 387, 1516, 1868, 2523, 2536, 2609, 2825
PspPI	GGNCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
PspPPI	RGGWCCY	3 cut(s)	43, 385, 2521
PsrI	GAACNNNNNNTAC	2 cut(s)	769, 801
PstI	CTGCAG	3 cut(s)	505, 846, 2589
PstNI	CAGNNNCTG	3 cut(s)	861, 1499, 2872
PsuI	RGATCY	7 cut(s)	838, 943, 1010, 1300, 1327, 1699, 1729
PteI	GCGCGC	2 cut(s)	663, 665
RruI	TCGCGA	2 cut(s)	718, 2319
RsaI	GTAC	4 cut(s)	536, 2551, 2570, 2609
RsaNI	GTAC	4 cut(s)	535, 2550, 2569, 2608
RseI	CAYNNNNRTG	1 cut(s)	2082
SacI	GAGCTC	2 cut(s)	1339, 2640
SalI	GTCGAC	1 cut(s)	1679
SaqAI	TTAA	6 cut(s)	495, 980, 990, 1341, 2772, 3054
SatI	GCNGC	6 cut(s)	504, 884, 1173, 1187, 2867, 2926
Sau96I	GGNCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
SchI	GAGTC	7 cut(s)	455, 536, 605, 722, 1740, 1861, 2318
ScrFI	CCNGG	8 cut(s)	41, 153, 894, 1656, 1857, 1989, 2623, 2930
SduI	GDGCHC	4 cut(s)	1339, 1871, 2114, 2640
SfcI	CTRYAG	5 cut(s)	201, 501, 842, 2585, 3024
SfuI	TTCGAA	1 cut(s)	214
SinI	GGWCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
SmiMI	CAYNNNNRTG	1 cut(s)	2082
SmlI	CTYRAG	3 cut(s)	1304, 1340, 1843
SmoI	CTYRAG	3 cut(s)	1304, 1340, 1843
SpeI	ACTAGT	1 cut(s)	2794
SseBI	AGGCCT	1 cut(s)	553
SsiI	CCGC	5 cut(s)	224, 612, 1109, 1146, 3043
SspI	AATATT	1 cut(s)	3158
SspMI	CTAG	6 cut(s)	297, 1464, 1973, 2052, 2259, 2795
SstI	GAGCTC	2 cut(s)	1339, 2640
StuI	AGGCCT	1 cut(s)	553
StyD4I	CCNGG	8 cut(s)	39, 151, 892, 1654, 1855, 1987, 2621, 2928
StyI	CCWWGG	5 cut(s)	575, 758, 1509, 1599, 1709
TaiI	ACGT	2 cut(s)	427, 787
TaqI	TCGA	9 cut(s)	177, 214, 260, 464, 1680, 1741, 1931, 2497, 3039
TatI	WGTACW	3 cut(s)	534, 2549, 2568
TfiI	GAWTC	9 cut(s)	254, 953, 1860, 2087, 2303, 2485, 2491, 2807, 3086
Tru1I	TTAA	6 cut(s)	495, 980, 990, 1341, 2772, 3054
Tru9I	TTAA	6 cut(s)	495, 980, 990, 1341, 2772, 3054
TseFI	GTSAC	6 cut(s)	521, 1649, 1834, 1968, 2543, 3005
TseI	GCWGC	6 cut(s)	503, 883, 1172, 1186, 2866, 2925
Tsp45I	GTSAC	6 cut(s)	521, 1649, 1834, 1968, 2543, 3005
TspGWI	ACGGA	5 cut(s)	44, 528, 946, 1167, 2993
Vha464I	CTTAAG	1 cut(s)	1340
VpaK11BI	GGWCC	6 cut(s)	43, 385, 1644, 2521, 2534, 3108
VspI	ATTAAT	1 cut(s)	990
XceI	RCATGY	1 cut(s)	2569
XcmI	CCANNNNNNNNNTGG	1 cut(s)	56
XmiI	GTMKAC	1 cut(s)	1680
XmnI	GAANNNNTTC	1 cut(s)	210
XspI	CTAG	6 cut(s)	297, 1464, 1973, 2052, 2259, 2795
Zsp2I	ATGCAT	2 cut(s)	727, 2504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.