Rorug02G0516300

longifolia

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004546
Physical Location & Seq
Reverse (-)
64764614 .. 64767447
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0516300.1

Sequence Viewer

Length: 1032 bp
ATGGCCGGAGAAGGAGTATTCTGTCACCAGTGCCGCCAAAGGACCACGAAACGTGTGTTCTTGTGCAACAATGTCATGGGGAAACGCCAATGCAAGATCAAGATCTGCGAGCGGTGCCTCAAGAAAAGATATGTAGATATTACAGAGGAGGCGGCTGCTTCAGATGATTGGAAGCGTCCGAAATGCTGCGGGATCTGCAACTGCAGTGTGTGCATGAAGCGAGAGGGTAAAGAACCTCTTGGTCGAGCTGTGCCTGACTCAGAGAATCCTTCAGAGGAGGAGCCTGCGGGAGTGTCTGAGCCAAATAGAGAGGAAGAAAACCAAATAGAAGATGTTGGCAAGGGCAAGAGGAAGGCCAAGGCTCCTAGGGAACCTCGCAGAGTGAAGGCAAAGGAGGAAGTAGTTGAGCCTGAACTTCCTCTGCCTTTGGGGGTTCCCTTAACAACTGTTGCGGGCATTGAACTACATCCTCAGGATGTAGGACATGCCCTACAGTTTATAGAGTTCTGTGTGGCCCTTGAAGAGGTTCTTAAGTTCAGCAAAGAAGATGCTGAATCTCTTCTCAGAGACCTAATGCATCAACGATACTCCTCAGTAGTCCGATTCCAGGCCAAACTCCTGTCTAAGATACAGAAGATGGGGGACGTAAAAGAATCACTGAGCAATTTCATAGAAAGCCAAAAAGAAATATTCTCTAAAAGTAAGATCGCAGCAAAACGAAAGTATCTGCTGCGAAGGCTAAGTGAAGCAGCTCAAGATCATGCAGAGTTGCTTGAAGCAAAAGCCTTGAAATCAAAGCCTTATCTGTTCGATGCTGATAGTCGTGCATTTTGGAGATTGACAGGCTATGGTGATGGAGATGATGTGCTGCTTCAAGGACGAGGCCTTATGTCTGAAACCTTGTCTCAAATACTTCCTTCAAGTTCTGGAACACTTCCTTCAAGTTCTGGAAGTAAGAAAGAAGAGAAGCTTGAAAATGCGACTACTGGAAGCAGAGAAGAAGACATACCCAAAAGTAGTGCAGAAGAGTAA

Protein Analysis

343

Amino Acids

38.4

Weight (kDa)

7.9

Isoelectric Point (pI)

61.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 4 - 84 2.5e-19 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 114
AccBSI CCGCTC 1 cut(s) 112
AciI CCGC 6 cut(s) 34, 112, 152, 189, 287, 452
AclWI GGATC 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 144, 255
AfiI CCNNNNNNNGG 2 cut(s) 523, 607
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 8 cut(s) 461, 521, 776, 790, 875, 921, 942, 974
AjnI CCWGG 1 cut(s) 606
AluBI AGCT 3 cut(s) 248, 752, 970
AluI AGCT 3 cut(s) 248, 752, 970
Alw26I GTCTC 2 cut(s) 561, 909
AlwI GGATC 1 cut(s) 200
AoxI GGCC 5 cut(s) 3, 354, 513, 609, 883
ApeKI GCWGC 6 cut(s) 155, 186, 710, 730, 749, 868
Asp700I GAANNNNTTC 2 cut(s) 525, 558
AspA2I CCTAGG 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 42, 514
AsuHPI GGTGA 2 cut(s) 17, 863
AvaII GGWCC 1 cut(s) 42
AvrII CCTAGG 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 471
BaeI ACNNNNGTAYC 2 cut(s) 707, 740
BanI GGYRCC 1 cut(s) 114
BarI GAAGNNNNNNTAC 2 cut(s) 990, 1022
BbsI GAAGAC 1 cut(s) 1008
BbvI GCAGC 6 cut(s) 142, 173, 717, 722, 761, 855
BccI CCATC 2 cut(s) 631, 848
BciT130I CCWGG 1 cut(s) 608
BcoDI GTCTC 2 cut(s) 561, 909
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 2 cut(s) 202, 491
BfrI CTTAAG 1 cut(s) 530
BglII AGATCT 1 cut(s) 102
BisI GCNGC 8 cut(s) 34, 153, 156, 187, 711, 731, 750, 869
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 8 cut(s) 35, 154, 157, 188, 712, 732, 751, 870
Bme1390I CCNGG 1 cut(s) 608
Bme18I GGWCC 1 cut(s) 42
BmgT120I GGNCC 2 cut(s) 42, 514
BmiI GGNNCC 5 cut(s) 116, 282, 363, 372, 435
BmrFI CCNGG 1 cut(s) 608
BmsI GCATC 3 cut(s) 538, 586, 802
BpiI GAAGAC 1 cut(s) 1008
BpuEI CTTGAG 2 cut(s) 104, 738
BsaBI GATNNNNATC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 561
BsaJI CCNNGG 2 cut(s) 357, 365
BsaXI ACNNNNNCTCC 3 cut(s) 30, 849, 879
Bsc4I CCNNNNNNNGG 2 cut(s) 523, 607
Bse1I ACTGG 2 cut(s) 28, 991
Bse21I CCTNAGG 1 cut(s) 471
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 2 cut(s) 357, 365
BseGI GGATG 2 cut(s) 466, 481
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 2 cut(s) 523, 607
BseMII CTCAG 6 cut(s) 273, 288, 485, 577, 606, 650
BseNI ACTGG 2 cut(s) 28, 991
BseRI GAGGAG 4 cut(s) 161, 290, 293, 580
BseXI GCAGC 6 cut(s) 142, 173, 717, 722, 761, 855
BshFI GGCC 5 cut(s) 5, 356, 515, 611, 885
BshNI GGYRCC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 2 cut(s) 523, 607
BsmAI GTCTC 2 cut(s) 561, 909
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 5 cut(s) 5, 356, 515, 611, 885
Bso31I GGTCTC 1 cut(s) 561
Bsp143I GATC 5 cut(s) 96, 102, 192, 705, 757
BspACI CCGC 6 cut(s) 34, 112, 152, 189, 287, 452
BspANI GGCC 5 cut(s) 5, 356, 515, 611, 885
BspCNI CTCAG 6 cut(s) 272, 289, 484, 576, 605, 651
BspLI GGNNCC 5 cut(s) 116, 282, 363, 372, 435
BspMAI CTGCAG 1 cut(s) 206
BspPI GGATC 1 cut(s) 200
BspT107I GGYRCC 1 cut(s) 114
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 561
BsrBI CCGCTC 1 cut(s) 112
BsrI ACTGG 2 cut(s) 28, 991
BssECI CCNNGG 2 cut(s) 357, 365
BssMI GATC 5 cut(s) 96, 102, 192, 705, 757
BssT1I CCWWGG 2 cut(s) 357, 365
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 2 cut(s) 448, 495
Bst6I CTCTTC 4 cut(s) 516, 564, 957, 1020
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 110, 285, 454
BstDEI CTNAG 8 cut(s) 259, 297, 471, 563, 592, 624, 659, 740
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 2 cut(s) 466, 481
BstKTI GATC 5 cut(s) 99, 105, 195, 708, 760
BstMAI GTCTC 2 cut(s) 561, 909
BstMBI GATC 5 cut(s) 96, 102, 192, 705, 757
BstMWI GCNNNNNNNGC 4 cut(s) 114, 195, 210, 736
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 606
BstSFI CTRYAG 2 cut(s) 202, 491
BstV1I GCAGC 6 cut(s) 142, 173, 717, 722, 761, 855
BstV2I GAAGAC 1 cut(s) 1008
BstX2I RGATCY 2 cut(s) 102, 192
BstYI RGATCY 2 cut(s) 102, 192
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 5 cut(s) 5, 356, 515, 611, 885
BtsCI GGATG 2 cut(s) 466, 481
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 3 cut(s) 35, 211, 656
Cac8I GCNNGC 3 cut(s) 110, 285, 454
Cfr13I GGNCC 2 cut(s) 42, 514
CseI GACGC 1 cut(s) 164
CviAII CATG 4 cut(s) 76, 214, 485, 761
DdeI CTNAG 8 cut(s) 259, 297, 471, 563, 592, 624, 659, 740
DpnI GATC 5 cut(s) 98, 104, 194, 707, 759
DpnII GATC 5 cut(s) 96, 102, 192, 705, 757
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 516, 564, 957, 1020
EarI CTCTTC 4 cut(s) 516, 564, 957, 1020
Eco130I CCWWGG 2 cut(s) 357, 365
Eco147I AGGCCT 1 cut(s) 885
Eco31I GGTCTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 144, 255
Eco81I CCTNAGG 1 cut(s) 471
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 606
EcoT14I CCWWGG 2 cut(s) 357, 365
EcoT22I ATGCAT 1 cut(s) 579
ErhI CCWWGG 2 cut(s) 357, 365
FaeI CATG 4 cut(s) 79, 217, 488, 764
FalI AAGNNNNNCTT 6 cut(s) 222, 254, 513, 545, 954, 986
FaqI GGGAC 1 cut(s) 656
FatI CATG 4 cut(s) 75, 213, 484, 760
FauI CCCGC 3 cut(s) 182, 280, 445
Fnu4HI GCNGC 8 cut(s) 34, 153, 156, 187, 711, 731, 750, 869
FokI GGATG 2 cut(s) 453, 488
Fsp4HI GCNGC 8 cut(s) 34, 153, 156, 187, 711, 731, 750, 869
FspBI CTAG 1 cut(s) 366
GluI GCNGC 8 cut(s) 34, 153, 156, 187, 711, 731, 750, 869
HaeIII GGCC 5 cut(s) 5, 356, 515, 611, 885
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 164
Hin1II CATG 4 cut(s) 79, 217, 488, 764
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 5 cut(s) 257, 265, 554, 603, 653
HpaII CCGG 1 cut(s) 6
HphI GGTGA 2 cut(s) 17, 863
Hpy188I TCNGA 8 cut(s) 163, 180, 262, 274, 298, 566, 602, 895
Hpy188III TCNNGA 6 cut(s) 100, 121, 473, 755, 927, 948
HpyAV CCTTC 7 cut(s) 5, 279, 346, 379, 729, 927, 948
HpyCH4III ACNGT 2 cut(s) 448, 495
HpyCH4IV ACGT 2 cut(s) 52, 645
HpyCH4V TGCA 9 cut(s) 66, 93, 198, 204, 213, 577, 764, 827, 1022
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 195, 210, 736
HpyF3I CTNAG 8 cut(s) 259, 297, 471, 563, 592, 624, 659, 740
HpySE526I ACGT 2 cut(s) 52, 645
Hsp92II CATG 4 cut(s) 79, 217, 488, 764
Kzo9I GATC 5 cut(s) 96, 102, 192, 705, 757
LmnI GCTCC 2 cut(s) 280, 367
Lsp1109I GCAGC 6 cut(s) 142, 173, 717, 722, 761, 855
LweI GCATC 3 cut(s) 538, 586, 802
MaeI CTAG 1 cut(s) 366
MaeII ACGT 2 cut(s) 52, 645
MaeIII GTNAC 1 cut(s) 23
MalI GATC 5 cut(s) 98, 104, 194, 707, 759
MbiI CCGCTC 1 cut(s) 112
MboI GATC 5 cut(s) 96, 102, 192, 705, 757
MboII GAAGA 9 cut(s) 326, 341, 533, 551, 557, 646, 974, 1010, 1013
MflI RGATCY 2 cut(s) 102, 192
MluCI AATT 1 cut(s) 664
MlyI GAGTC 1 cut(s) 251
Mph1103I ATGCAT 1 cut(s) 579
MroXI GAANNNNTTC 2 cut(s) 525, 558
MseI TTAA 2 cut(s) 440, 531
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 608
MvaI CCWGG 1 cut(s) 608
MwoI GCNNNNNNNGC 4 cut(s) 114, 195, 210, 736
NdeII GATC 5 cut(s) 96, 102, 192, 705, 757
NlaIII CATG 4 cut(s) 79, 217, 488, 764
NlaIV GGNNCC 5 cut(s) 116, 282, 363, 372, 435
NmuCI GTSAC 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 488
PceI AGGCCT 1 cut(s) 885
PdmI GAANNNNTTC 2 cut(s) 525, 558
PfeI GAWTC 4 cut(s) 265, 554, 603, 653
PkrI GCNGC 8 cut(s) 35, 154, 157, 188, 712, 732, 751, 870
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 5 cut(s) 116, 282, 363, 372, 435
PspPI GGNCC 2 cut(s) 42, 514
PstI CTGCAG 1 cut(s) 206
PsuI RGATCY 2 cut(s) 102, 192
SaqAI TTAA 2 cut(s) 440, 531
SatI GCNGC 8 cut(s) 34, 153, 156, 187, 711, 731, 750, 869
Sau3AI GATC 5 cut(s) 96, 102, 192, 705, 757
Sau96I GGNCC 2 cut(s) 42, 514
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 608
SfaNI GCATC 3 cut(s) 538, 586, 802
SfcI CTRYAG 2 cut(s) 202, 491
SinI GGWCC 1 cut(s) 42
SmlI CTYRAG 3 cut(s) 119, 530, 753
SmoI CTYRAG 3 cut(s) 119, 530, 753
Sse9I AATT 1 cut(s) 664
SseBI AGGCCT 1 cut(s) 885
SsiI CCGC 6 cut(s) 34, 112, 152, 189, 287, 452
SspI AATATT 1 cut(s) 690
SspMI CTAG 1 cut(s) 366
StuI AGGCCT 1 cut(s) 885
StyD4I CCNGG 1 cut(s) 606
StyI CCWWGG 2 cut(s) 357, 365
TaaI ACNGT 2 cut(s) 448, 495
TaiI ACGT 2 cut(s) 55, 648
TaqI TCGA 2 cut(s) 244, 810
TasI AATT 1 cut(s) 664
TauI GCSGC 2 cut(s) 36, 155
TfiI GAWTC 4 cut(s) 265, 554, 603, 653
Tru1I TTAA 2 cut(s) 440, 531
Tru9I TTAA 2 cut(s) 440, 531
TscAI CASTG 3 cut(s) 35, 211, 663
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 6 cut(s) 155, 186, 710, 730, 749, 868
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 230, 658
TspRI CASTG 3 cut(s) 35, 211, 663
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 1 cut(s) 42
XagI CCTNNNNNAGG 1 cut(s) 521
XceI RCATGY 1 cut(s) 488
XmaJI CCTAGG 1 cut(s) 365
XmnI GAANNNNTTC 2 cut(s) 525, 558
XspI CTAG 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.