Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004546

Physical Location & Seq

Genomic Coordinates Reverse (-)

4964089 .. 4968728

Gene Structure 4640 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1061300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3156 bp

ATGTCTGCAAAGATTCTGCATTCTCTAAAGGATGAGAACCGGGATCTCCATAAGCAAATTGGGTGCATGAGTGGAATTTTTCAGCTCTTTGATCGCCACCATTTCCTCGCCGGCAGGCGTATCAATGGCCAGAACCACAAGAGACTCTCTCCAGGTGAGAATTGCAACCACGCAGTAGAACCAAGAACAGTGCAGAAGGCTACCGAAAAAATTCGAAAAAAGGCGGTGAAAGAGAAACAACGAAACTCCACAGAATCATCGAAAACCACTGTTGCTTCTTCTTCGTGTTCATCTAGCTTTTCGTCTCTTGAACATCAAAAGGCAGCTGAGCAAGAACCATCTTTGTCCGGCCAAACCATTTCCAACGACAAACACGCTCGGGACCTATCTCTGAACCAACCAAATGTTTTGATGCACATACGCCGGAGATCTGTTGATCTGCAAGATCTTGTCAAAGACTCAATGTACAGAGAAGCCCGTGGCATTTCAGTTAAACCTGCATCTAAAGATGGAGCCGGTCATGCATTGAAGTACATAGACTCCCCGAGGCCTTCACCAATAAAATCCACCAAGACCAGGTTTTCTGGTGGTAACGAGTCATTTCAAGTTCCTGGAAAGCTTCGGGTCGCACCTTGGAGTTCAAATGAAGAGAAGGATGGTGGTACACGTTTTGTACCAAAAGATGCTCGGAGGTTCTCTTATGATGGAAGAGAGTCACGCGATGCATCAAAATCCACCGTAAAACTCAAAGAAGTCCCAAGGCTTTCTTTGGACAGCAAAGAACGTTCCATAAGGAGGGGTTGTAGCCCAGAAACAAAAGCAAATTACTTCATCAAGGATCTGCAGAGAGAATATGGGAACAGCAACAAAATGCTTGACCTGCAGCATGAACCTGGAAGCGCTAAAAGACCGTCTAATGTAGTTGCAAAGTTGATGGGACTGGATCTATCAGATTCCGTGTCAACCACTGATAGTCCATTAAGGCTGATTAATACTGGCACATCTGATAGATCTGATCCCTTCTCAAGATCATCAAGAGCAACCACTGAAAACAAGCAAGATATTTTTTCCGGAATACTCCTTGCGAATGCCAAGAAAGACTTCAGCTCACCCCAGAGGAGAAGTACTGATTCAGTCATGAAGCCCACCTCGAATTCCAAGTTTCCAATAGAAACAGCTCCTTGGAGGCAGCCGCACAGAAGCAAAGGTTCTCAACGATCAGCTTTCAAGCATCAAGAAGAACCGACAAAAGCTCCAAACTCATCATCTTCAGTATATGGTGAAATGGAGAAAAGGCTAGCAGATCTTGAGTTTAAAAAATCAGGAAAGGATCTCAGAGCTCTTAAACAAATACTTGAAGCGATGCAGAAGACGAAAGGAATGACAGATACAAGAAAGGATCACGCCTTAAATTTTGCATCTCCAATAAGCAACGAAAGCATTTCCTCTGACAGCACAGTAAAAGCAAGCCAGAGAAATCTACAAAGTAACATATCAGTTCCTGCCACGGCCAAGGGTTCCCAATCTCCAAAGAGCTATAAATCACCCATCGTCATAATGAAACCAGCTAAACTCATTGATAAAATCCACAGCTCTGCTACCATGGTGGATTCCATGAATGACACGTTAGGTCTTCGGAAGCTTCGGACTAGTGACCCTGGTGATAACAGAAAGGGATTGGTCGACAATAATACAGCCAAAGATCCGACACCAAGGAACAGTCATGTCAGAGATCCTTTCAATCGACACCTTCATTCCATTGATGATAATTCTAACGCAAGAACTTTGAAATCTGCACAAAAACCAAAGGCATCGCAAACTATGAGTGAAGAACAAGTCATCAGCTCAAGTAGGAGTTCAAAAATCACTAGCCCGAAAATGCAACAGAGAAGACTTGGATTGGAAAAGCAATCTCCTCCAAGCACGCCATCATTGAATTCAAGCATGACCAGAAGACAACACACTAGGCAGTCATTAGAAGCAACCACCCCAGGTAGAAAACTTGGGCATAAGACACCCAGTTTGCATCAAAGTAATGCGCAACTGAGGGAGACTAGCACCAAGATGAGAGGGATGAGTCATCACGATGATTCCACTTCACAGCAATCTGGAAGCACCATCAGCTTGACCTCGCACACAGACACAGCCACAAGCGTTCACGAATCAGATAGGATCACAGGCACGCATTTCCAGCAGCATAACCAAAAAATAAAGCACCCAGCAGTGGGGTTGATTGATGATAGGTCAACGGCAGAAACTAGGAAAGCTTCCTCTGAACAACCAAGCCCTGTCTCTGTTCTTGATTCTACATTCTATCGGGATGACTCACCATCTCCAGTGAAGAAGATATCAAATGCCTTCAAAGACGATGAAGCACAAATTTTGGATATAGTAGGGTATGACCCAATGAACCAATCTCTGTTATCCAACAACACAATGCCCAGTCCTGGGGCAGAAATTGATCATAAAAGATTAGAGAACCTGATTCAGAATCATCGATGCATGAGCAGGACACACGAGGACCCCATCACTGGTCCCCTCTGTGACAGTACAAATCCAGACCACATGTACATTTCAGAAATATTGCTGGCATCAGGTATTCTGGAATATCTCGAATCTCCCTGGACAACCAGTGAGCTCCACACATCAGACCACCTGATCAACCCTAATTTGTTCCTTGCACTGGAAGATATCAGAACAAATACAATGCCTTCTGATCTCCAGCCCAAACCTGATGAGAAACTTCAAAGAAAACTAGTATTTGAGGTCATTAATGAATTCCTTGTTCAAAAACTAGTGGTGGAAGATTCTTTGAAGCAGTGGTTCCCACCACACAAGCTGGCAGATTGGGAACCAAGAAGCCAGCAGCTTCTGAAAGAATTGTGTTCAGAGGTTGATCAGCTACAAAGAAAGAACTTGAATGGCAGCCTGGATGACGAGGATGAAAGTTTGAGAAACATCTTGTTGGAAGATTTCACGGATCAAGCAAAGAATTGGACAGAGTGTGACAGTGAAATTCCAGGTGTAGTGTTGGATGTTGAGCGATTGATCTTTAAAGATTTGATAAATGAAATTGTGAATGGTGATTCAATTAGCCTGCATGGTTGGTCTGGTGGGCATTGCAGGCAACTGTTTTCTGGGAAGGGGCTGTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0008017 GO:0008092 GO:0008150 GO:0015631 GO:0022603 GO:0022604 GO:0040008 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051510 GO:0051513 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

1052

Amino Acids

117.46

Weight (kDa)

9.28

Isoelectric Point (pI)

60.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4378	PF14309	854 - 1024	2.9e-28	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004546)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46710 FvH4_6g46710 FvH4_6g46710 FvH4_6g46710
malus_domestica	MD09G1068400.v1.1 MD17G1061300.v1.1
prunus_persica	Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1
pyrus_communis	pycom12433g00110 pycom12433g00120 pycom17g06120
rosa_chinensis	RchiOBHm_Chr2g0165601
rosa_laevigata	RLG00000021542
rosa_multiflora	Rmu_co8268985.1_g000001 Rmu_co8395029.1_g000001 Rmu_sc0005425.1_g000021 Rmu_sc0035818.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085700
rosa_rugosa	Rorug02G0516300 Rorug02G0516400 Rorug02G0516400 Rorug02G0516400
rosa_samantha	Rh2AG582500 Rh2BG594600 Rh2CG564500 Rh2DG604500
rosa_wichuraiana	Rw0G016060 Rw2G048590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2040
Acc36I	ACCTGC	2 cut(s)	505, 888
AccI	GTMKAC	1 cut(s)	1683
AccII	CGCG	1 cut(s)	720
AccIII	TCCGGA	1 cut(s)	1070
AciI	CCGC	2 cut(s)	224, 1193
AclI	AACGTT	1 cut(s)	784
AcoI	YGGCCR	3 cut(s)	127, 349, 1509
AcsI	RAATTY	8 cut(s)	75, 210, 1153, 1411, 1936, 2379, 2777, 3015
AcuI	CTGAAG	2 cut(s)	1087, 1254
AfaI	GTAC	7 cut(s)	467, 533, 664, 675, 1126, 2551, 2570
AfeI	AGCGCT	1 cut(s)	901
AfiI	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
AflIII	ACRYGT	3 cut(s)	665, 1623, 2565
AhlI	ACTAGT	3 cut(s)	1649, 2755, 2794
AjuI	GAANNNNNNNTTGG	2 cut(s)	1085, 1117
Alw21I	GWGCWC	2 cut(s)	1342, 2640
Alw26I	GTCTC	4 cut(s)	136, 309, 2045, 2296
AlwNI	CAGNNNCTG	2 cut(s)	1502, 2872
Ama87I	CYCGRG	2 cut(s)	378, 544
Aor13HI	TCCGGA	1 cut(s)	1070
Aor51HI	AGCGCT	1 cut(s)	901
AoxI	GGCC	4 cut(s)	127, 349, 548, 1509
ApeKI	GCWGC	6 cut(s)	323, 883, 1189, 2194, 2866, 2925
ApoI	RAATTY	8 cut(s)	75, 210, 1153, 1411, 1936, 2379, 2777, 3015
ArsI	GACNNNNNNTTYG	2 cut(s)	2005, 2037
AseI	ATTAAT	2 cut(s)	990, 2772
Asp700I	GAANNNNTTC	2 cut(s)	210, 1100
AspLEI	GCGC	2 cut(s)	902, 2041
AspS9I	GGNCC	3 cut(s)	382, 2521, 2534
AsuC2I	CCSGG	1 cut(s)	41
AsuHPI	GGTGA	9 cut(s)	167, 238, 546, 1101, 1292, 1536, 1673, 2319, 3095
AsuII	TTCGAA	1 cut(s)	214
AsuNHI	GCTAGC	1 cut(s)	1297
AvaI	CYCGRG	2 cut(s)	378, 544
AvaII	GGWCC	3 cut(s)	382, 2521, 2534
BalI	TGGCCA	1 cut(s)	129
BanII	GRGCYC	2 cut(s)	1342, 2640
BauI	CACGAG	1 cut(s)	2516
BbsI	GAAGAC	4 cut(s)	1376, 1625, 1897, 1960
Bbv12I	GWGCWC	2 cut(s)	1342, 2640
BbvI	GCAGC	6 cut(s)	335, 895, 1201, 2206, 2878, 2937
BceAI	ACGGC	2 cut(s)	1524, 2265
BclI	TGATCA	3 cut(s)	2461, 2658, 2896
BcnI	CCSGG	1 cut(s)	41
BcoDI	GTCTC	4 cut(s)	136, 309, 2045, 2296
BcuI	ACTAGT	3 cut(s)	1649, 2755, 2794
BfaI	CTAG	9 cut(s)	294, 1298, 1650, 1869, 1965, 2055, 2259, 2756, 2795
BfmI	CTRYAG	2 cut(s)	842, 881
BfoI	RGCGCY	1 cut(s)	903
BfuAI	ACCTGC	2 cut(s)	505, 888
BglII	AGATCT	4 cut(s)	428, 445, 1010, 1303
BisI	GCNGC	7 cut(s)	324, 884, 1190, 1193, 2195, 2867, 2926
BlpI	GCTNAGC	1 cut(s)	327
BlsI	GCNGC	7 cut(s)	325, 885, 1191, 1194, 2196, 2868, 2927
BmcAI	AGTACT	1 cut(s)	1126
Bme18I	GGWCC	3 cut(s)	382, 2521, 2534
BmeT110I	CYCGRG	2 cut(s)	378, 544
BmgT120I	GGNCC	3 cut(s)	382, 2521, 2534
BmiI	GGNNCC	7 cut(s)	383, 514, 1519, 2523, 2536, 2825, 2853
BmrI	ACTGGG	2 cut(s)	2013, 2436
BmtI	GCTAGC	1 cut(s)	1301
BmuI	ACTGGG	2 cut(s)	2013, 2436
BpiI	GAAGAC	4 cut(s)	1376, 1625, 1897, 1960
BplI	GAGNNNNNCTC	2 cut(s)	133, 165
BpmI	CTGGAG	3 cut(s)	135, 2319, 2705
Bpu1102I	GCTNAGC	1 cut(s)	327
Bpu14I	TTCGAA	1 cut(s)	214
BpuEI	CTTGAG	3 cut(s)	1009, 1328, 1831
BpuMI	CCSGG	1 cut(s)	41
Bsa29I	ATCGAT	1 cut(s)	2497
BsaWI	WCCGGW	1 cut(s)	1070
BsaXI	ACNNNNNCTCC	6 cut(s)	524, 554, 787, 817, 1237, 1267
Bsc4I	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
Bse118I	RCCGGY	2 cut(s)	110, 515
Bse1I	ACTGG	8 cut(s)	945, 1000, 2019, 2336, 2442, 2536, 2631, 2688
Bse3DI	GCAATG	1 cut(s)	3118
BseAI	TCCGGA	1 cut(s)	1070
BseCI	ATCGAT	1 cut(s)	2497
BseGI	GGATG	7 cut(s)	37, 661, 2079, 2326, 2938, 2947, 3040
BseLI	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
BseMI	GCAATG	1 cut(s)	3118
BseMII	CTCAG	3 cut(s)	318, 1348, 2036
BseNI	ACTGG	8 cut(s)	945, 1000, 2019, 2336, 2442, 2536, 2631, 2688
BseRI	GAGGAG	2 cut(s)	1132, 1905
BseXI	GCAGC	6 cut(s)	335, 895, 1201, 2206, 2878, 2937
BseYI	CCCAGC	1 cut(s)	2218
BsgI	GTGCAG	2 cut(s)	212, 1779
Bsh1236I	CGCG	1 cut(s)	720
BshFI	GGCC	4 cut(s)	129, 351, 550, 1511
BshVI	ATCGAT	1 cut(s)	2497
BsiHKAI	GWGCWC	2 cut(s)	1342, 2640
BsiHKCI	CYCGRG	2 cut(s)	378, 544
BsiSI	CCGG	6 cut(s)	40, 111, 348, 424, 516, 1071
BslFI	GGGAC	4 cut(s)	395, 740, 951, 2520
BslI	CCNNNNNNNGG	8 cut(s)	576, 795, 2224, 2225, 2447, 2448, 2531, 2683
BsmAI	GTCTC	4 cut(s)	136, 309, 2045, 2296
BsmBI	CGTCTC	1 cut(s)	309
BsmFI	GGGAC	4 cut(s)	395, 740, 951, 2520
BsmI	GAATGC	2 cut(s)	19, 1093
BsnI	GGCC	4 cut(s)	129, 351, 550, 1511
BsoBI	CYCGRG	2 cut(s)	378, 544
Bsp119I	TTCGAA	1 cut(s)	214
Bsp1286I	GDGCHC	2 cut(s)	1342, 2640
Bsp13I	TCCGGA	1 cut(s)	1070
Bsp1407I	TGTACA	2 cut(s)	465, 2568
Bsp1720I	GCTNAGC	1 cut(s)	327
Bsp19I	CCATGG	1 cut(s)	1602
BspACI	CCGC	2 cut(s)	224, 1193
BspANI	GGCC	4 cut(s)	129, 351, 550, 1511
BspCNI	CTCAG	3 cut(s)	319, 1347, 2037
BspDI	ATCGAT	1 cut(s)	2497
BspEI	TCCGGA	1 cut(s)	1070
BspFNI	CGCG	1 cut(s)	720
BspHI	TCATGA	1 cut(s)	1137
BspLI	GGNNCC	7 cut(s)	383, 514, 1519, 2523, 2536, 2825, 2853
BspMAI	CTGCAG	2 cut(s)	846, 885
BspMI	ACCTGC	2 cut(s)	505, 888
BspOI	GCTAGC	1 cut(s)	1301
BspT104I	TTCGAA	1 cut(s)	214
BsrDI	GCAATG	1 cut(s)	3118
BsrFI	RCCGGY	2 cut(s)	110, 515
BsrGI	TGTACA	2 cut(s)	465, 2568
BsrI	ACTGG	8 cut(s)	945, 1000, 2019, 2336, 2442, 2536, 2631, 2688
BssAI	RCCGGY	2 cut(s)	110, 515
BssSI	CACGAG	1 cut(s)	2516
BssT1I	CCWWGG	6 cut(s)	632, 758, 1181, 1512, 1602, 1712
Bst2BI	CACGAG	1 cut(s)	2516
Bst4CI	ACNGT	9 cut(s)	190, 271, 739, 912, 1459, 1721, 2549, 3011, 3132
Bst6I	CTCTTC	2 cut(s)	642, 703
BstAUI	TGTACA	2 cut(s)	465, 2568
BstBI	TTCGAA	1 cut(s)	214
BstDEI	CTNAG	3 cut(s)	327, 1334, 2045
BstDSI	CCRYGG	3 cut(s)	478, 1506, 1602
BstF5I	GGATG	7 cut(s)	37, 661, 2079, 2326, 2938, 2947, 3040
BstFNI	CGCG	1 cut(s)	720
BstH2I	RGCGCY	1 cut(s)	903
BstHHI	GCGC	2 cut(s)	902, 2041
BstMAI	GTCTC	4 cut(s)	136, 309, 2045, 2296
BstMWI	GCNNNNNNNGC	6 cut(s)	521, 880, 1437, 2121, 2191, 3124
BstNSI	RCATGY	1 cut(s)	2569
BstSFI	CTRYAG	2 cut(s)	842, 881
BstUI	CGCG	1 cut(s)	720
BstV1I	GCAGC	6 cut(s)	335, 895, 1201, 2206, 2878, 2937
BstV2I	GAAGAC	4 cut(s)	1376, 1625, 1897, 1960
Bsu15I	ATCGAT	1 cut(s)	2497
BsuRI	GGCC	4 cut(s)	129, 351, 550, 1511
BsuTUI	ATCGAT	1 cut(s)	2497
BtgI	CCRYGG	3 cut(s)	478, 1506, 1602
BtgZI	GCGATG	3 cut(s)	735, 1376, 1797
BtsCI	GGATG	7 cut(s)	37, 661, 2079, 2326, 2938, 2947, 3040
BtsI	GCAGTG	2 cut(s)	2229, 2825
BveI	ACCTGC	2 cut(s)	505, 888
CaiI	CAGNNNCTG	2 cut(s)	1502, 2872
CciI	TCATGA	1 cut(s)	1137
CfoI	GCGC	2 cut(s)	902, 2041
Cfr10I	RCCGGY	2 cut(s)	110, 515
Cfr13I	GGNCC	3 cut(s)	382, 2521, 2534
ClaI	ATCGAT	1 cut(s)	2497
CsiI	ACCWGGT	1 cut(s)	575
Csp6I	GTAC	7 cut(s)	466, 532, 663, 674, 1125, 2550, 2569
CviQI	GTAC	7 cut(s)	466, 532, 663, 674, 1125, 2550, 2569
DdeI	CTNAG	3 cut(s)	327, 1334, 2045
DraI	TTTAAA	2 cut(s)	1315, 3055
EaeI	YGGCCR	3 cut(s)	127, 349, 1509
Eam1104I	CTCTTC	2 cut(s)	642, 703
EarI	CTCTTC	2 cut(s)	642, 703
Ecl136II	GAGCTC	2 cut(s)	1340, 2638
Eco130I	CCWWGG	6 cut(s)	632, 758, 1181, 1512, 1602, 1712
Eco147I	AGGCCT	1 cut(s)	550
Eco24I	GRGCYC	2 cut(s)	1342, 2640
Eco32I	GATATC	2 cut(s)	2349, 2692
Eco47I	GGWCC	3 cut(s)	382, 2521, 2534
Eco47III	AGCGCT	1 cut(s)	901
Eco53kI	GAGCTC	2 cut(s)	1340, 2638
Eco57I	CTGAAG	2 cut(s)	1087, 1254
Eco88I	CYCGRG	2 cut(s)	378, 544
EcoICRI	GAGCTC	2 cut(s)	1340, 2638
EcoO109I	RGGNCCY	2 cut(s)	382, 2521
EcoRI	GAATTC	3 cut(s)	1153, 1936, 2777
EcoRV	GATATC	2 cut(s)	2349, 2692
EcoT14I	CCWWGG	6 cut(s)	632, 758, 1181, 1512, 1602, 1712
EcoT22I	ATGCAT	3 cut(s)	526, 727, 2504
EcoT38I	GRGCYC	2 cut(s)	1342, 2640
ErhI	CCWWGG	6 cut(s)	632, 758, 1181, 1512, 1602, 1712
Esp3I	CGTCTC	1 cut(s)	309
FalI	AAGNNNNNCTT	4 cut(s)	751, 783, 1085, 1117
FaqI	GGGAC	4 cut(s)	395, 740, 951, 2520
FbaI	TGATCA	3 cut(s)	2461, 2658, 2896
FblI	GTMKAC	1 cut(s)	1683
Fnu4HI	GCNGC	7 cut(s)	324, 884, 1190, 1193, 2195, 2867, 2926
FokI	GGATG	7 cut(s)	44, 668, 2086, 2333, 2945, 2954, 3047
FriOI	GRGCYC	2 cut(s)	1342, 2640
Fsp4HI	GCNGC	7 cut(s)	324, 884, 1190, 1193, 2195, 2867, 2926
FspBI	CTAG	9 cut(s)	294, 1298, 1650, 1869, 1965, 2055, 2259, 2756, 2795
FspI	TGCGCA	1 cut(s)	2040
GlaI	GCGC	2 cut(s)	901, 2040
GluI	GCNGC	7 cut(s)	324, 884, 1190, 1193, 2195, 2867, 2926
GsaI	CCCAGC	1 cut(s)	2222
GsuI	CTGGAG	3 cut(s)	135, 2319, 2705
HaeII	RGCGCY	1 cut(s)	903
HaeIII	GGCC	4 cut(s)	129, 351, 550, 1511
HapII	CCGG	6 cut(s)	40, 111, 348, 424, 516, 1071
HhaI	GCGC	2 cut(s)	902, 2041
Hin6I	GCGC	2 cut(s)	900, 2039
HinP1I	GCGC	2 cut(s)	900, 2039
HincII	GTYRAC	3 cut(s)	963, 1684, 2247
HindII	GTYRAC	3 cut(s)	963, 1684, 2247
HindIII	AAGCTT	3 cut(s)	617, 1640, 2265
HpaII	CCGG	6 cut(s)	40, 111, 348, 424, 516, 1071
HphI	GGTGA	9 cut(s)	167, 238, 546, 1101, 1292, 1536, 1673, 2319, 3095
Hpy166II	GTNNAC	5 cut(s)	665, 963, 1684, 2158, 2247
Hpy8I	GTNNAC	5 cut(s)	665, 963, 1684, 2158, 2247
HpyAV	CCTTC	8 cut(s)	190, 561, 646, 1030, 1760, 2368, 2721, 3136
HpyCH4III	ACNGT	9 cut(s)	190, 271, 739, 912, 1459, 1721, 2549, 3011, 3132
HpyCH4IV	ACGT	3 cut(s)	667, 784, 1625
HpyF10VI	GCNNNNNNNGC	6 cut(s)	521, 880, 1437, 2121, 2191, 3124
HpyF3I	CTNAG	3 cut(s)	327, 1334, 2045
HpySE526I	ACGT	3 cut(s)	667, 784, 1625
HspAI	GCGC	2 cut(s)	900, 2039
Kpn2I	TCCGGA	1 cut(s)	1070
KroI	GCCGGC	1 cut(s)	110
KroNI	GCCGGC	1 cut(s)	112
Ksp22I	TGATCA	3 cut(s)	2461, 2658, 2896
LmnI	GCTCC	4 cut(s)	512, 1183, 1258, 2643
Lsp1109I	GCAGC	6 cut(s)	335, 895, 1201, 2206, 2878, 2937
MabI	ACCWGGT	1 cut(s)	575
MaeI	CTAG	9 cut(s)	294, 1298, 1650, 1869, 1965, 2055, 2259, 2756, 2795
MaeII	ACGT	3 cut(s)	667, 784, 1625
MaeIII	GTNAC	6 cut(s)	590, 714, 1487, 1652, 2543, 3005
MhlI	GDGCHC	2 cut(s)	1342, 2640
MlsI	TGGCCA	1 cut(s)	129
MluNI	TGGCCA	1 cut(s)	129
MlyI	GAGTC	7 cut(s)	138, 452, 533, 605, 722, 2086, 2318
MmeI	TCCRAC	5 cut(s)	387, 1730, 2451, 2946, 3012
Mox20I	TGGCCA	1 cut(s)	129
Mph1103I	ATGCAT	3 cut(s)	526, 727, 2504
MroI	TCCGGA	1 cut(s)	1070
MroNI	GCCGGC	1 cut(s)	110
MroXI	GAANNNNTTC	2 cut(s)	210, 1100
MscI	TGGCCA	1 cut(s)	129
MseI	TTAA	8 cut(s)	492, 980, 990, 1314, 1344, 1409, 2772, 3054
MslI	CAYNNNNRTG	2 cut(s)	2063, 2085
Msp20I	TGGCCA	1 cut(s)	129
MspA1I	CMGCKG	1 cut(s)	326
MspI	CCGG	6 cut(s)	40, 111, 348, 424, 516, 1071
Mva1269I	GAATGC	2 cut(s)	19, 1093
MvnI	CGCG	1 cut(s)	720
MwoI	GCNNNNNNNGC	6 cut(s)	521, 880, 1437, 2121, 2191, 3124
NaeI	GCCGGC	1 cut(s)	112
NciI	CCSGG	1 cut(s)	41
NcoI	CCATGG	1 cut(s)	1602
NgoMIV	GCCGGC	1 cut(s)	110
NheI	GCTAGC	1 cut(s)	1297
NlaIV	GGNNCC	7 cut(s)	383, 514, 1519, 2523, 2536, 2825, 2853
NmuCI	GTSAC	4 cut(s)	714, 1652, 2543, 3005
NsbI	TGCGCA	1 cut(s)	2040
NsiI	ATGCAT	3 cut(s)	526, 727, 2504
NspI	RCATGY	1 cut(s)	2569
NspV	TTCGAA	1 cut(s)	214
PagI	TCATGA	1 cut(s)	1137
PceI	AGGCCT	1 cut(s)	550
PciI	ACATGT	1 cut(s)	2565
PctI	GAATGC	2 cut(s)	19, 1093
PdiI	GCCGGC	1 cut(s)	112
PdmI	GAANNNNTTC	2 cut(s)	210, 1100
PfoI	TCCNGGA	1 cut(s)	610
PkrI	GCNGC	7 cut(s)	325, 885, 1191, 1194, 2196, 2868, 2927
PleI	GAGTC	7 cut(s)	138, 452, 533, 604, 721, 2085, 2318
PpsI	GAGTC	7 cut(s)	138, 452, 533, 604, 721, 2085, 2318
PpuMI	RGGWCCY	2 cut(s)	382, 2521
PscI	ACATGT	1 cut(s)	2565
PshBI	ATTAAT	2 cut(s)	990, 2772
Psp124BI	GAGCTC	2 cut(s)	1342, 2640
Psp1406I	AACGTT	1 cut(s)	784
Psp5II	RGGWCCY	2 cut(s)	382, 2521
PspFI	CCCAGC	1 cut(s)	2218
PspN4I	GGNNCC	7 cut(s)	383, 514, 1519, 2523, 2536, 2825, 2853
PspPI	GGNCC	3 cut(s)	382, 2521, 2534
PspPPI	RGGWCCY	2 cut(s)	382, 2521
PstI	CTGCAG	2 cut(s)	846, 885
PstNI	CAGNNNCTG	2 cut(s)	1502, 2872
PvuII	CAGCTG	1 cut(s)	326
RsaI	GTAC	7 cut(s)	467, 533, 664, 675, 1126, 2551, 2570
RsaNI	GTAC	7 cut(s)	466, 532, 663, 674, 1125, 2550, 2569
RseI	CAYNNNNRTG	2 cut(s)	2063, 2085
SacI	GAGCTC	2 cut(s)	1342, 2640
SalI	GTCGAC	1 cut(s)	1682
SaqAI	TTAA	8 cut(s)	492, 980, 990, 1314, 1344, 1409, 2772, 3054
SatI	GCNGC	7 cut(s)	324, 884, 1190, 1193, 2195, 2867, 2926
Sau96I	GGNCC	3 cut(s)	382, 2521, 2534
ScaI	AGTACT	1 cut(s)	1126
SchI	GAGTC	7 cut(s)	138, 452, 533, 605, 722, 2086, 2318
SduI	GDGCHC	2 cut(s)	1342, 2640
SexAI	ACCWGGT	1 cut(s)	575
SfcI	CTRYAG	2 cut(s)	842, 881
SfuI	TTCGAA	1 cut(s)	214
SinI	GGWCC	3 cut(s)	382, 2521, 2534
SmiMI	CAYNNNNRTG	2 cut(s)	2063, 2085
SmlI	CTYRAG	3 cut(s)	1024, 1307, 1846
SmoI	CTYRAG	3 cut(s)	1024, 1307, 1846
SpeI	ACTAGT	3 cut(s)	1649, 2755, 2794
SseBI	AGGCCT	1 cut(s)	550
SsiI	CCGC	2 cut(s)	224, 1193
SspI	AATATT	1 cut(s)	2583
SspMI	CTAG	9 cut(s)	294, 1298, 1650, 1869, 1965, 2055, 2259, 2756, 2795
SstI	GAGCTC	2 cut(s)	1342, 2640
StuI	AGGCCT	1 cut(s)	550
StyI	CCWWGG	6 cut(s)	632, 758, 1181, 1512, 1602, 1712
TaaI	ACNGT	9 cut(s)	190, 271, 739, 912, 1459, 1721, 2549, 3011, 3132
TaiI	ACGT	3 cut(s)	670, 787, 1628
TaqI	TCGA	7 cut(s)	214, 260, 1151, 1683, 1744, 2497, 2613
TatI	WGTACW	5 cut(s)	465, 531, 1124, 2549, 2568
TauI	GCSGC	1 cut(s)	1195
Tru1I	TTAA	8 cut(s)	492, 980, 990, 1314, 1344, 1409, 2772, 3054
Tru9I	TTAA	8 cut(s)	492, 980, 990, 1314, 1344, 1409, 2772, 3054
TseFI	GTSAC	4 cut(s)	714, 1652, 2543, 3005
TseI	GCWGC	6 cut(s)	323, 883, 1189, 2194, 2866, 2925
Tsp45I	GTSAC	4 cut(s)	714, 1652, 2543, 3005
TspGWI	ACGGA	2 cut(s)	946, 2993
VpaK11BI	GGWCC	3 cut(s)	382, 2521, 2534
VspI	ATTAAT	2 cut(s)	990, 2772
XapI	RAATTY	8 cut(s)	75, 210, 1153, 1411, 1936, 2379, 2777, 3015
XceI	RCATGY	1 cut(s)	2569
XcmI	CCANNNNNNNNNTGG	1 cut(s)	56
XmiI	GTMKAC	1 cut(s)	1683
XmnI	GAANNNNTTC	2 cut(s)	210, 1100
XspI	CTAG	9 cut(s)	294, 1298, 1650, 1869, 1965, 2055, 2259, 2756, 2795
ZrmI	AGTACT	1 cut(s)	1126
Zsp2I	ATGCAT	3 cut(s)	526, 727, 2504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.