Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00750

Orthogroup ID

OG0004546

Physical Location & Seq

Genomic Coordinates Reverse (-)

86327 .. 92796

Gene Structure 6470 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G016060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGGAGCTAGTTACTATATATGAACGGGTTGAGATCTCAATTCTGATACTATATTTGAGGCAAATCCCATCTTTCTTCAACTCTTTACTGTTTTTTTTTAGTTTATATTTTGAAAAAGATGAGAATCGTGATCTCCAAAAGCAACTTGGGTGCATGAGCGGGATTTTTCAGCTGTTTGATCGCCGGCATTTCCTCTCCGGCCGGAGCATCAATGGCCACAGTCACAAGAGACTTCCTCCAGGTGAGAATGGCAAACAAGAAATAGATCCCAAAAGCAGAGCACTGCCCAAAGCTACAGATAAAAATCGAAAAAAGGCTGTCAAAGAGAGACGAAACTCCACAGAATCATCCACAACCACTGTTTCTTCATCTTCTTGTTCATCTAGCTTCTCATCATCTCTTGAATACAACAAAGCAGCTCAGCAAGAACAATCCTTGTCTGGCCAAACAATTTACAATGACAGACACACTCGGGACCTGTCCATGAACAAACCGAATATTTCAAAGCCCTTAAGCCAGCAATCATTTGACTCAACCTACAGAGAAGCCCGTGGTATTTCAGTCAGACCTGGAGGAAAAGATGGAGTAGGTCATAGATTGAAATACATAGACTCCCCAAGGCCTTCACAACCACTAAAACCCATCAAGCCAAGGGTTTCTGGTGGCGAGTCATTTCAAGTTCATGCCAAGCTTCGGGAAGCACATCGAAATTCCAACGAGGAGAAGGATGGTTGCATGCGATTTGCGCCAAAAGAAGCAAGGAGGCTGTCTTATGATGAAAGGGCATCACGAGATACAGTGAAATCCACAACAAAACTCAAAGAACTCCCAAGGCTTTCTTTGGACAGCAAAGAACGTTCCATTAGGAGGGCTTGTAGCCCTGAAACAAGATCAAGCCATTTCTTCAAGGATCTGCAGAGGGAGAATGGGAACTGTGACAAAATGCTTGACCTGCAGCCAGAACCCGGAAGTTCTAAAAGACCGAATGTTGTTGCAAAGTTGATGGGCTTGGATCTCTCAGATTCAGTGTCAACCAGTGTTACTCCATTGAGGCTGATTAGTACCTGTCCATCTGATAGATTTGATCCCTTGTCCAAATCATTGAGAAAAACAGATGAATCCAAGCAAGATGTTTTGTCTGGGTTCCCAAGAAATACGCAGAAGAGCTTCAGCTCACCCCAGAGGAGAAGTGCCAATTCAGTCATGCAGCCCACCGCAAATTCCAAGTTTCCAATAGAAACAGCTCCTTGGAGGCAGCCACATGGAAGCAAAGGTCTTCCTCAATCAACTTTCAACTGTCAAGAAGAACCTACAAAAGCACCAAAGTCATCACCTTCAGTATATGGTGAAATGGAGAAAAGACTAGCAGAACTAGAGTTCAAGAAATCAGGAAAGGACCTCCGAGCTCTTAAACAGATACTTGAAGCAATGCAGAAGAGCAAGCAAAACTCAGAGGACAAAAAAGAAGCCTCAAATTTTGCCTCTCAAGTAAGCAACAAAAGTGGTTTGTGTGAGAGCACAATATCAGCAAGCAAGAGAAATACAAAACGTAGCCCATCACTTCCAGCCACAGCCATGGGTTCCAAATCCCCAAAGAGTTGCAAATCACCAATCATCATCATGAAACCCGGTAAACTCATGGAGAAAACACACAACTCTGCTTCCACGGTGATCTCCATGGACAACAGATCATGTCCGCGGAGGCTTCAGACTAGCAACCCTGGTGACAACAGAAAGGAATTGCTTGACAGGAAAGCCAAAGATTTGACTCCAAGAAACATTCATACTAGTGATTCTTTCAATGGACGTCTTCGCTCCACTGATAAGAATTCTAATGTGAGAACTTCTAAAGCTGCACAAAAACCAAAGATGCCCCACAGTGAAGAAAATTCCCCGGGCCCAAGTAGGAGCATCACGAGCCCAAGACTGCAAAACAGAAGATTTGGATTGGAGAAGCAATCTGCTCCAACCACTCTATCATCAGATTCAAGCATGACCAGAAGGCAACACAGTAGGCAATCATTAGAAGCAAGCACCCCGGGTAGAAAACTCGGGCCAAAATCTCCCAGATTTCATCAAAGCAATTGCCAACTGAGCGAGACTAGCACCAGTACAAGAGCTATGAGTCACCAAGATGATACCACTTCTCAGCAATCTGAAAGCAACACCAGCTTGGCCTCACATGCAGACACAGAAGCCACAAGCATTCATCAGTCAGATGAGATTAGAGATACTTACATGAAGCAGCACAGCCAAAAGCAAAACAGGCCAGCAGTAGGGTTGAGTGATGATAGGTTAACAGCAGAAACTGGGAAAGCTTCCTTAGAACAACCAAGTCCTATCTCCGTTCTTGATTCTACATTCTATAGGGACGACTCGCCTTCTCCTGTGAAGAAGATATCAAATGCCTTTAAAGATGATGGTGGGCAAAATCTGGATGAAGCAGAGTATGGACCAATGGACCTAGGTCTGATATCCAATAGTACAACGCCCAGTCTTGGTGCAGAGATTGACCATAAAAGGTTAGAGAACCTGAAGCACCTGATTCATAATCATCGACATATGAGCAGCACACATGAAGAACCCATCTTCGGGCACATTACTTTCCTCTGTGACCGCTCAAATCCAGATCACATGTACATTTCAGATATATTGCTAGCATCAGGAATTCTTAGGTATCTCGAATCTGCGTGGACAACCATTGAGCTCCAGACATTAGAACACCTGATCAACCCTAATTTGTTCCTTGCACTGGAAGAAATTAGGGCAGATATAGAACCTTGGGATGATGGAAAGAGATGTGAAAAGATTCTCCAGTCCCAATCAGATGAAAAAATTCAAAGAAAGCTAGTGTTCGATGTCGTTAAAGAATTCCTAGTTCAAAAATTAGTTGTGGAGAACTCTTTCAAGCAGTGGTTCTCACCAAACAAGCTGGCAGAAGGGAAACCAAGAGGGCAGCAGCTTTTGACAGAATTGTGCTCCCAGGTTGATCAGCTACAAAGACGCAACTTGAATGGCAACCTAGATGATGAAGATGAAAGTTTGACAAGCATCTTGTTGGAAAATTTCATGGATCAATCACAGAATTGGACAGTGTGTGATGGTGAAATTCCGAGTGTAGTGTTGGATGTTGAGCGATTGATCTTTAAAGACTTGATAACTGAAATTGTGAGCAGTGATGCAGTTGACCATGTGGGTTGGTCTGGTGGGCATTGTAGGCAACTGTTTTCCAAGAAGGGGCTCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0008017 GO:0008092 GO:0008150 GO:0015631 GO:0022603 GO:0022604 GO:0040008 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051510 GO:0051513 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4378 VARLMGL

Protein Analysis

1082

Amino Acids

121.73

Weight (kDa)

9.27

Isoelectric Point (pI)

62.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4378	PF14309	877 - 1055	2.3e-26	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004546)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46710 FvH4_6g46710 FvH4_6g46710 FvH4_6g46710
malus_domestica	MD09G1068400.v1.1 MD17G1061300.v1.1
prunus_persica	Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1 Prupe.3G252400_v2.0.a1
pyrus_communis	pycom12433g00110 pycom12433g00120 pycom17g06120
rosa_chinensis	RchiOBHm_Chr2g0165601
rosa_laevigata	RLG00000021542
rosa_multiflora	Rmu_co8268985.1_g000001 Rmu_co8395029.1_g000001 Rmu_sc0005425.1_g000021 Rmu_sc0035818.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085700
rosa_rugosa	Rorug02G0516300 Rorug02G0516400 Rorug02G0516400 Rorug02G0516400
rosa_samantha	Rh2AG582500 Rh2BG594600 Rh2CG564500 Rh2DG604500
rosa_wichuraiana	Rw0G016060 Rw2G048590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1810
Acc36I	ACCTGC	1 cut(s)	960
AccBSI	CCGCTC	2 cut(s)	159, 2619
AccII	CGCG	1 cut(s)	1699
AciI	CCGC	5 cut(s)	159, 1215, 1697, 1699, 2617
AclI	AACGTT	1 cut(s)	856
AclWI	GGATC	5 cut(s)	260, 918, 1020, 1079, 3081
AcoI	YGGCCR	3 cut(s)	199, 214, 442
AcuI	CTGAAG	4 cut(s)	1153, 1320, 1691, 2555
AcyI	GRCGYC	1 cut(s)	1807
AfaI	GTAC	4 cut(s)	1063, 2113, 2485, 2639
AfiI	CCNNNNNNNGG	9 cut(s)	693, 867, 965, 2276, 2498, 2591, 2592, 2752, 3237
AflII	CTTAAG	1 cut(s)	511
AflIII	ACRYGT	1 cut(s)	2634
AhlI	ACTAGT	1 cut(s)	1787
AjnI	CCWGG	4 cut(s)	238, 568, 1720, 2982
Alw21I	GWGCWC	5 cut(s)	283, 1408, 1520, 2709, 2981
Alw26I	GTCTC	3 cut(s)	223, 322, 2093
AlwI	GGATC	5 cut(s)	260, 918, 1020, 1079, 3081
AlwNI	CAGNNNCTG	1 cut(s)	2309
Ama87I	CYCGRG	4 cut(s)	471, 1894, 2038, 2051
AoxI	GGCC	8 cut(s)	199, 214, 442, 620, 1897, 2054, 2175, 2267
ApaI	GGGCCC	1 cut(s)	1901
ApeKI	GCWGC	9 cut(s)	416, 955, 1207, 1255, 1853, 2245, 2568, 2956, 2959
Asp700I	GAANNNNTTC	4 cut(s)	1166, 2808, 2835, 2903
AspA2I	CCTAGG	1 cut(s)	2464
AspLEI	GCGC	1 cut(s)	748
AspS9I	GGNCC	7 cut(s)	475, 1396, 1897, 1898, 2054, 2453, 2461
AsuC2I	CCSGG	6 cut(s)	966, 1629, 1895, 1896, 2039, 2040
AsuNHI	GCTAGC	1 cut(s)	2656
AvaI	CYCGRG	4 cut(s)	471, 1894, 2038, 2051
AvaII	GGWCC	4 cut(s)	475, 1396, 2453, 2461
AvrII	CCTAGG	1 cut(s)	2464
BaeGI	GKGCMC	2 cut(s)	1901, 2598
BalI	TGGCCA	2 cut(s)	216, 444
BanII	GRGCYC	5 cut(s)	1408, 1901, 1922, 2709, 3243
BauI	CACGAG	2 cut(s)	789, 1915
BbsI	GAAGAC	2 cut(s)	1268, 1802
Bbv12I	GWGCWC	5 cut(s)	283, 1408, 1520, 2709, 2981
BbvI	GCAGC	9 cut(s)	428, 967, 1219, 1267, 1840, 2257, 2580, 2968, 2971
BciT130I	CCWGG	4 cut(s)	240, 570, 1722, 2984
BclI	TGATCA	2 cut(s)	2727, 2989
BcnI	CCSGG	6 cut(s)	966, 1629, 1895, 1896, 2039, 2040
BcoDI	GTCTC	3 cut(s)	223, 322, 2093
BcuI	ACTAGT	1 cut(s)	1787
BfmI	CTRYAG	5 cut(s)	294, 538, 914, 953, 2365
BfrI	CTTAAG	1 cut(s)	511
BfuAI	ACCTGC	1 cut(s)	960
BglII	AGATCT	1 cut(s)	33
BisI	GCNGC	9 cut(s)	417, 956, 1208, 1256, 1854, 2246, 2569, 2957, 2960
BlnI	CCTAGG	1 cut(s)	2464
BlpI	GCTNAGC	1 cut(s)	420
BlsI	GCNGC	9 cut(s)	418, 957, 1209, 1257, 1855, 2247, 2570, 2958, 2961
Bme18I	GGWCC	4 cut(s)	475, 1396, 2453, 2461
BmeT110I	CYCGRG	4 cut(s)	471, 1894, 2038, 2051
BmgT120I	GGNCC	7 cut(s)	475, 1396, 1897, 1898, 2054, 2453, 2461
BmiI	GGNNCC	4 cut(s)	476, 1145, 1582, 1899
BmrI	ACTGGG	2 cut(s)	2319, 2487
BmsI	GCATC	7 cut(s)	216, 794, 1860, 1920, 2669, 3060, 3169
BmtI	GCTAGC	1 cut(s)	2660
BmuI	ACTGGG	2 cut(s)	2319, 2487
BoxI	GACNNNNGTC	1 cut(s)	2466
BpiI	GAAGAC	2 cut(s)	1268, 1802
BplI	GAGNNNNNCTC	2 cut(s)	220, 252
BpmI	CTGGAG	4 cut(s)	222, 591, 2693, 2798
Bpu1102I	GCTNAGC	1 cut(s)	420
BpuEI	CTTGAG	1 cut(s)	1470
BpuMI	CCSGG	6 cut(s)	966, 1629, 1895, 1896, 2039, 2040
BsaBI	GATNNNNATC	2 cut(s)	123, 303
BsaHI	GRCGYC	1 cut(s)	1807
BsaXI	ACNNNNNCTCC	4 cut(s)	596, 626, 859, 889
Bsc4I	CCNNNNNNNGG	9 cut(s)	693, 867, 965, 2276, 2498, 2591, 2592, 2752, 3237
Bse118I	RCCGGY	1 cut(s)	183
Bse1I	ACTGG	6 cut(s)	1035, 2109, 2314, 2493, 2757, 2815
Bse3DI	GCAATG	1 cut(s)	1434
Bse8I	GATNNNNATC	2 cut(s)	123, 303
BseBI	CCWGG	4 cut(s)	240, 570, 1722, 2984
BseGI	GGATG	5 cut(s)	347, 733, 2443, 2791, 3133
BseJI	GATNNNNATC	2 cut(s)	123, 303
BseLI	CCNNNNNNNGG	9 cut(s)	693, 867, 965, 2276, 2498, 2591, 2592, 2752, 3237
BseMI	GCAATG	1 cut(s)	1434
BseMII	CTCAG	5 cut(s)	434, 1032, 1464, 2084, 2162
BseNI	ACTGG	6 cut(s)	1035, 2109, 2314, 2493, 2757, 2815
BseRI	GAGGAG	2 cut(s)	734, 1198
BseSI	GKGCMC	2 cut(s)	1901, 2598
BseX3I	CGGCCG	1 cut(s)	199
BseXI	GCAGC	9 cut(s)	428, 967, 1219, 1267, 1840, 2257, 2580, 2968, 2971
BsgI	GTGCAG	2 cut(s)	1839, 2523
Bsh1236I	CGCG	1 cut(s)	1699
Bsh1285I	CGRYCG	1 cut(s)	202
BshFI	GGCC	8 cut(s)	201, 216, 444, 622, 1899, 2056, 2177, 2269
BsiEI	CGRYCG	1 cut(s)	202
BsiHKAI	GWGCWC	5 cut(s)	283, 1408, 1520, 2709, 2981
BsiHKCI	CYCGRG	4 cut(s)	471, 1894, 2038, 2051
BsiSI	CCGG	7 cut(s)	184, 198, 202, 966, 1629, 1895, 2039
BslFI	GGGAC	3 cut(s)	488, 2384, 2803
BslI	CCNNNNNNNGG	9 cut(s)	693, 867, 965, 2276, 2498, 2591, 2592, 2752, 3237
BsmAI	GTCTC	3 cut(s)	223, 322, 2093
BsmBI	CGTCTC	1 cut(s)	322
BsmFI	GGGAC	3 cut(s)	488, 2384, 2803
BsmI	GAATGC	1 cut(s)	2205
BsnI	GGCC	8 cut(s)	201, 216, 444, 622, 1899, 2056, 2177, 2269
BsoBI	CYCGRG	4 cut(s)	471, 1894, 2038, 2051
Bsp120I	GGGCCC	1 cut(s)	1897
Bsp1286I	GDGCHC	9 cut(s)	283, 1408, 1520, 1901, 1922, 2598, 2709, 2981, 3243
Bsp1407I	TGTACA	1 cut(s)	2637
Bsp1720I	GCTNAGC	1 cut(s)	420
Bsp19I	CCATGG	2 cut(s)	1575, 1677
BspACI	CCGC	5 cut(s)	159, 1215, 1697, 1699, 2617
BspANI	GGCC	8 cut(s)	201, 216, 444, 622, 1899, 2056, 2177, 2269
BspCNI	CTCAG	5 cut(s)	433, 1031, 1463, 2085, 2161
BspFNI	CGCG	1 cut(s)	1699
BspHI	TCATGA	1 cut(s)	1620
BspLI	GGNNCC	4 cut(s)	476, 1145, 1582, 1899
BspMAI	CTGCAG	2 cut(s)	918, 957
BspMI	ACCTGC	1 cut(s)	960
BspOI	GCTAGC	1 cut(s)	2660
BspPI	GGATC	5 cut(s)	260, 918, 1020, 1079, 3081
BspQI	GCTCTTC	2 cut(s)	1157, 1430
BspTI	CTTAAG	1 cut(s)	511
BsrBI	CCGCTC	2 cut(s)	159, 2619
BsrDI	GCAATG	1 cut(s)	1434
BsrFI	RCCGGY	1 cut(s)	183
BsrGI	TGTACA	1 cut(s)	2637
BsrI	ACTGG	6 cut(s)	1035, 2109, 2314, 2493, 2757, 2815
BssAI	RCCGGY	1 cut(s)	183
BssNI	GRCGYC	1 cut(s)	1807
BssSI	CACGAG	2 cut(s)	789, 1915
BssT1I	CCWWGG	8 cut(s)	617, 650, 830, 1247, 1575, 1677, 2464, 2780
Bst2BI	CACGAG	2 cut(s)	789, 1915
Bst2UI	CCWGG	4 cut(s)	240, 570, 1722, 2984
Bst6I	CTCTTC	2 cut(s)	1157, 1430
BstACI	GRCGYC	1 cut(s)	1807
BstAFI	CTTAAG	1 cut(s)	511
BstAUI	TGTACA	1 cut(s)	2637
BstC8I	GCNNGC	9 cut(s)	185, 518, 737, 1442, 1531, 2032, 2271, 2658, 2934
BstDEI	CTNAG	7 cut(s)	420, 1018, 1450, 2093, 2148, 2323, 2672
BstDSI	CCRYGG	5 cut(s)	550, 1575, 1665, 1677, 1697
BstF5I	GGATG	5 cut(s)	347, 733, 2443, 2791, 3133
BstFNI	CGCG	1 cut(s)	1699
BstHHI	GCGC	1 cut(s)	748
BstMAI	GTCTC	3 cut(s)	223, 322, 2093
BstMCI	CGRYCG	1 cut(s)	202
BstNI	CCWGG	4 cut(s)	240, 570, 1722, 2984
BstNSI	RCATGY	3 cut(s)	739, 2186, 2638
BstPAI	GACNNNNGTC	1 cut(s)	2466
BstSFI	CTRYAG	5 cut(s)	294, 538, 914, 953, 2365
BstSLI	GKGCMC	2 cut(s)	1901, 2598
BstUI	CGCG	1 cut(s)	1699
BstV1I	GCAGC	9 cut(s)	428, 967, 1219, 1267, 1840, 2257, 2580, 2968, 2971
BstV2I	GAAGAC	2 cut(s)	1268, 1802
BstX2I	RGATCY	4 cut(s)	33, 265, 910, 1012
BstXI	CCANNNNNNTGG	1 cut(s)	1576
BstYI	RGATCY	4 cut(s)	33, 265, 910, 1012
BstZI	CGGCCG	1 cut(s)	199
BsuRI	GGCC	8 cut(s)	201, 216, 444, 622, 1899, 2056, 2177, 2269
BtgI	CCRYGG	5 cut(s)	550, 1575, 1665, 1677, 1697
BtsCI	GGATG	5 cut(s)	347, 733, 2443, 2791, 3133
BtsI	GCAGTG	3 cut(s)	281, 2918, 3181
BveI	ACCTGC	1 cut(s)	960
Cac8I	GCNNGC	9 cut(s)	185, 518, 737, 1442, 1531, 2032, 2271, 2658, 2934
CaiI	CAGNNNCTG	1 cut(s)	2309
CciI	TCATGA	1 cut(s)	1620
CfoI	GCGC	1 cut(s)	748
Cfr10I	RCCGGY	1 cut(s)	183
Cfr13I	GGNCC	7 cut(s)	475, 1396, 1897, 1898, 2054, 2453, 2461
Cfr42I	CCGCGG	1 cut(s)	1700
Cfr9I	CCCGGG	2 cut(s)	1894, 2038
CseI	GACGC	1 cut(s)	3012
Csp6I	GTAC	4 cut(s)	1062, 2112, 2484, 2638
CspCI	CAANNNNNGTGG	2 cut(s)	1483, 1518
CviQI	GTAC	4 cut(s)	1062, 2112, 2484, 2638
DdeI	CTNAG	7 cut(s)	420, 1018, 1450, 2093, 2148, 2323, 2672
DraI	TTTAAA	2 cut(s)	2413, 3148
EaeI	YGGCCR	3 cut(s)	199, 214, 442
EagI	CGGCCG	1 cut(s)	199
Eam1104I	CTCTTC	2 cut(s)	1157, 1430
EarI	CTCTTC	2 cut(s)	1157, 1430
Ecl136II	GAGCTC	2 cut(s)	1406, 2707
EclXI	CGGCCG	1 cut(s)	199
Eco130I	CCWWGG	8 cut(s)	617, 650, 830, 1247, 1575, 1677, 2464, 2780
Eco147I	AGGCCT	1 cut(s)	622
Eco24I	GRGCYC	5 cut(s)	1408, 1901, 1922, 2709, 3243
Eco32I	GATATC	2 cut(s)	2400, 2475
Eco47I	GGWCC	4 cut(s)	475, 1396, 2453, 2461
Eco52I	CGGCCG	1 cut(s)	199
Eco53kI	GAGCTC	2 cut(s)	1406, 2707
Eco57I	CTGAAG	4 cut(s)	1153, 1320, 1691, 2555
Eco88I	CYCGRG	4 cut(s)	471, 1894, 2038, 2051
EcoICRI	GAGCTC	2 cut(s)	1406, 2707
EcoO109I	RGGNCCY	2 cut(s)	475, 1396
EcoRI	GAATTC	3 cut(s)	1828, 2667, 2870
EcoRII	CCWGG	4 cut(s)	238, 568, 1720, 2982
EcoRV	GATATC	2 cut(s)	2400, 2475
EcoT14I	CCWWGG	8 cut(s)	617, 650, 830, 1247, 1575, 1677, 2464, 2780
EcoT38I	GRGCYC	5 cut(s)	1408, 1901, 1922, 2709, 3243
ErhI	CCWWGG	8 cut(s)	617, 650, 830, 1247, 1575, 1677, 2464, 2780
Esp3I	CGTCTC	1 cut(s)	322
FalI	AAGNNNNNCTT	2 cut(s)	823, 855
FaqI	GGGAC	3 cut(s)	488, 2384, 2803
FauI	CCCGC	1 cut(s)	152
FauNDI	CATATG	1 cut(s)	2562
FbaI	TGATCA	2 cut(s)	2727, 2989
Fnu4HI	GCNGC	9 cut(s)	417, 956, 1208, 1256, 1854, 2246, 2569, 2957, 2960
FokI	GGATG	5 cut(s)	334, 740, 2450, 2798, 3140
FriOI	GRGCYC	5 cut(s)	1408, 1901, 1922, 2709, 3243
Fsp4HI	GCNGC	9 cut(s)	417, 956, 1208, 1256, 1854, 2246, 2569, 2957, 2960
GlaI	GCGC	1 cut(s)	747
GluI	GCNGC	9 cut(s)	417, 956, 1208, 1256, 1854, 2246, 2569, 2957, 2960
GsuI	CTGGAG	4 cut(s)	222, 591, 2693, 2798
HaeIII	GGCC	8 cut(s)	201, 216, 444, 622, 1899, 2056, 2177, 2269
HapII	CCGG	7 cut(s)	184, 198, 202, 966, 1629, 1895, 2039
HgaI	GACGC	1 cut(s)	3012
HhaI	GCGC	1 cut(s)	748
Hin1I	GRCGYC	1 cut(s)	1807
Hin6I	GCGC	1 cut(s)	746
HinP1I	GCGC	1 cut(s)	746
HincII	GTYRAC	3 cut(s)	1032, 2298, 3187
HindII	GTYRAC	3 cut(s)	1032, 2298, 3187
HindIII	AAGCTT	2 cut(s)	689, 2316
HpaI	GTTAAC	1 cut(s)	2298
HpaII	CCGG	7 cut(s)	184, 198, 202, 966, 1629, 1895, 2039
Hpy166II	GTNNAC	5 cut(s)	1032, 1634, 2298, 2694, 3187
Hpy8I	GTNNAC	5 cut(s)	1032, 1634, 2298, 2694, 3187
HpyAV	CCTTC	7 cut(s)	633, 718, 1344, 1995, 2391, 2933, 3229
HpyCH4IV	ACGT	3 cut(s)	856, 1549, 1807
HpyF3I	CTNAG	7 cut(s)	420, 1018, 1450, 2093, 2148, 2323, 2672
HpySE526I	ACGT	3 cut(s)	856, 1549, 1807
Hsp92I	GRCGYC	1 cut(s)	1807
HspAI	GCGC	1 cut(s)	746
KroI	GCCGGC	1 cut(s)	183
KroNI	GCCGGC	1 cut(s)	185
Ksp22I	TGATCA	2 cut(s)	2727, 2989
KspAI	GTTAAC	1 cut(s)	2298
KspI	CCGCGG	1 cut(s)	1700
LguI	GCTCTTC	2 cut(s)	1157, 1430
LmnI	GCTCC	8 cut(s)	4, 204, 1249, 1820, 1908, 1969, 2712, 2984
Lsp1109I	GCAGC	9 cut(s)	428, 967, 1219, 1267, 1840, 2257, 2580, 2968, 2971
LweI	GCATC	7 cut(s)	216, 794, 1860, 1920, 2669, 3060, 3169
MaeII	ACGT	3 cut(s)	856, 1549, 1807
MaeIII	GTNAC	7 cut(s)	10, 221, 935, 1039, 1724, 2126, 2612
MbiI	CCGCTC	2 cut(s)	159, 2619
MfeI	CAATTG	1 cut(s)	2083
MflI	RGATCY	4 cut(s)	33, 265, 910, 1012
MhlI	GDGCHC	9 cut(s)	283, 1408, 1520, 1901, 1922, 2598, 2709, 2981, 3243
MlsI	TGGCCA	2 cut(s)	216, 444
MluNI	TGGCCA	2 cut(s)	216, 444
MlyI	GAGTC	6 cut(s)	524, 605, 677, 1762, 2134, 2369
MmeI	TCCRAC	4 cut(s)	738, 1991, 3039, 3105
Mox20I	TGGCCA	2 cut(s)	216, 444
MroNI	GCCGGC	1 cut(s)	183
MroXI	GAANNNNTTC	4 cut(s)	1166, 2808, 2835, 2903
MscI	TGGCCA	2 cut(s)	216, 444
MseI	TTAA	6 cut(s)	512, 1410, 2297, 2412, 2865, 3147
MslI	CAYNNNNRTG	4 cut(s)	1574, 1619, 1788, 2133
Msp20I	TGGCCA	2 cut(s)	216, 444
MspA1I	CMGCKG	2 cut(s)	172, 1699
MspCI	CTTAAG	1 cut(s)	511
MspI	CCGG	7 cut(s)	184, 198, 202, 966, 1629, 1895, 2039
MunI	CAATTG	1 cut(s)	2083
Mva1269I	GAATGC	1 cut(s)	2205
MvaI	CCWGG	4 cut(s)	240, 570, 1722, 2984
MvnI	CGCG	1 cut(s)	1699
NaeI	GCCGGC	1 cut(s)	185
NciI	CCSGG	6 cut(s)	966, 1629, 1895, 1896, 2039, 2040
NcoI	CCATGG	2 cut(s)	1575, 1677
NdeI	CATATG	1 cut(s)	2562
NgoMIV	GCCGGC	1 cut(s)	183
NheI	GCTAGC	1 cut(s)	2656
NlaIV	GGNNCC	4 cut(s)	476, 1145, 1582, 1899
NmuCI	GTSAC	5 cut(s)	221, 935, 1724, 2126, 2612
NspI	RCATGY	3 cut(s)	739, 2186, 2638
PaeI	GCATGC	1 cut(s)	739
PagI	TCATGA	1 cut(s)	1620
PceI	AGGCCT	1 cut(s)	622
PciI	ACATGT	1 cut(s)	2634
PciSI	GCTCTTC	2 cut(s)	1157, 1430
PctI	GAATGC	1 cut(s)	2205
PdiI	GCCGGC	1 cut(s)	185
PdmI	GAANNNNTTC	4 cut(s)	1166, 2808, 2835, 2903
PkrI	GCNGC	9 cut(s)	418, 957, 1209, 1257, 1855, 2247, 2570, 2958, 2961
PleI	GAGTC	6 cut(s)	524, 605, 676, 1762, 2133, 2369
PpsI	GAGTC	6 cut(s)	524, 605, 676, 1762, 2133, 2369
PpuMI	RGGWCCY	2 cut(s)	475, 1396
PscI	ACATGT	1 cut(s)	2634
PshAI	GACNNNNGTC	1 cut(s)	2466
Psp124BI	GAGCTC	2 cut(s)	1408, 2709
Psp1406I	AACGTT	1 cut(s)	856
Psp5II	RGGWCCY	2 cut(s)	475, 1396
Psp6I	CCWGG	4 cut(s)	238, 568, 1720, 2982
PspGI	CCWGG	4 cut(s)	238, 568, 1720, 2982
PspN4I	GGNNCC	4 cut(s)	476, 1145, 1582, 1899
PspOMI	GGGCCC	1 cut(s)	1897
PspPI	GGNCC	7 cut(s)	475, 1396, 1897, 1898, 2054, 2453, 2461
PspPPI	RGGWCCY	2 cut(s)	475, 1396
PstI	CTGCAG	2 cut(s)	918, 957
PstNI	CAGNNNCTG	1 cut(s)	2309
PsuI	RGATCY	4 cut(s)	33, 265, 910, 1012
PvuII	CAGCTG	1 cut(s)	172
RsaI	GTAC	4 cut(s)	1063, 2113, 2485, 2639
RsaNI	GTAC	4 cut(s)	1062, 2112, 2484, 2638
RseI	CAYNNNNRTG	4 cut(s)	1574, 1619, 1788, 2133
SacI	GAGCTC	2 cut(s)	1408, 2709
SacII	CCGCGG	1 cut(s)	1700
SapI	GCTCTTC	2 cut(s)	1157, 1430
SaqAI	TTAA	6 cut(s)	512, 1410, 2297, 2412, 2865, 3147
SatI	GCNGC	9 cut(s)	417, 956, 1208, 1256, 1854, 2246, 2569, 2957, 2960
Sau96I	GGNCC	7 cut(s)	475, 1396, 1897, 1898, 2054, 2453, 2461
SchI	GAGTC	6 cut(s)	524, 605, 677, 1762, 2134, 2369
SduI	GDGCHC	9 cut(s)	283, 1408, 1520, 1901, 1922, 2598, 2709, 2981, 3243
SfaNI	GCATC	7 cut(s)	216, 794, 1860, 1920, 2669, 3060, 3169
SfcI	CTRYAG	5 cut(s)	294, 538, 914, 953, 2365
Sfr303I	CCGCGG	1 cut(s)	1700
SgrBI	CCGCGG	1 cut(s)	1700
SinI	GGWCC	4 cut(s)	475, 1396, 2453, 2461
SmaI	CCCGGG	2 cut(s)	1896, 2040
SmiMI	CAYNNNNRTG	4 cut(s)	1574, 1619, 1788, 2133
SmlI	CTYRAG	2 cut(s)	511, 1485
SmoI	CTYRAG	2 cut(s)	511, 1485
SpeI	ACTAGT	1 cut(s)	1787
SphI	GCATGC	1 cut(s)	739
SseBI	AGGCCT	1 cut(s)	622
SsiI	CCGC	5 cut(s)	159, 1215, 1697, 1699, 2617
SspI	AATATT	1 cut(s)	499
SstI	GAGCTC	2 cut(s)	1408, 2709
StuI	AGGCCT	1 cut(s)	622
StyI	CCWWGG	8 cut(s)	617, 650, 830, 1247, 1575, 1677, 2464, 2780
TaiI	ACGT	3 cut(s)	859, 1552, 1810
TaqI	TCGA	5 cut(s)	307, 706, 2557, 2682, 2856
TaqII	GACCGA	1 cut(s)	997
TatI	WGTACW	3 cut(s)	2111, 2483, 2637
Tru1I	TTAA	6 cut(s)	512, 1410, 2297, 2412, 2865, 3147
Tru9I	TTAA	6 cut(s)	512, 1410, 2297, 2412, 2865, 3147
TseFI	GTSAC	5 cut(s)	221, 935, 1724, 2126, 2612
TseI	GCWGC	9 cut(s)	416, 955, 1207, 1255, 1853, 2245, 2568, 2956, 2959
Tsp45I	GTSAC	5 cut(s)	221, 935, 1724, 2126, 2612
TspGWI	ACGGA	1 cut(s)	2335
TspMI	CCCGGG	2 cut(s)	1894, 2038
Vha464I	CTTAAG	1 cut(s)	511
VpaK11BI	GGWCC	4 cut(s)	475, 1396, 2453, 2461
XceI	RCATGY	3 cut(s)	739, 2186, 2638
XcmI	CCANNNNNNNNNTGG	1 cut(s)	143
XmaI	CCCGGG	2 cut(s)	1894, 2038
XmaJI	CCTAGG	1 cut(s)	2464
XmnI	GAANNNNTTC	4 cut(s)	1166, 2808, 2835, 2903
ZraI	GACGTC	1 cut(s)	1808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.