pycom12433g00120

longifolia

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012433
OG0004546
Physical Location & Seq
Forward (+)
102739 .. 103341
603 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12433g00120.1

Sequence Viewer

Length: 603 bp
ATGTACATTTCAGATATATTGCTGGCATCAGGTATTCTCGGGTACCTTGAATCTGCCTGGACAACCAGTGAGCTGAACACATCAGACCACCTGATCGACCCTAATTTGTTCCTTGCACTGGAAGATATCAGAACAAATACAATGCCTTCTGATCTCCAGCCTAAACCTGATGAGAAAATTCAAAGAAAACAAGTATTTGAGGTTGTTAATGAATTCCTTGTTCACAAACTAGTGGTGGGAGATTCTTTGAAGCAGTGGTTCCCACCAAACAAGCTGGCAGATGGGAAACCAAGAAGCCAGCAGCTTCTGAAAGAATTGTGTTCAGAGGTTGATCAGCTACAGAGAACGAACTTGAATGGCAGCCTGGATGACGAGGATGAAAGTTTGAGAAACATCTTGTTAGAAGATTTCACGGATAAAGCAAAGAACTGGACAGAGTGTGACAGTGAAATTCCTTCTGTAGTGTTGGATGTCGAGCGGTTGATCGATCGTTTTAAAGATTTGATAAATGAAATTGTGAGTGATGATTCAGTTGGCCTGCATGGTTGGTCCGGTGGGCATTGCAGGCAACTTTTTTCTGAGAAGGGGCTAAGGAAATATTGA

Protein Analysis

201

Amino Acids

22.84

Weight (kDa)

4.66

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 2 - 171 1.3e-24 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 42
AccBSI CCGCTC 1 cut(s) 478
AciI CCGC 1 cut(s) 478
AcsI RAATTY 3 cut(s) 177, 212, 450
AfaI GTAC 2 cut(s) 5, 44
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 4 cut(s) 50, 182, 250, 355
AhlI ACTAGT 1 cut(s) 229
AjnI CCWGG 2 cut(s) 56, 363
AluBI AGCT 4 cut(s) 73, 274, 304, 337
AluI AGCT 4 cut(s) 73, 274, 304, 337
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 1 cut(s) 535
ApeKI GCWGC 2 cut(s) 301, 360
ApoI RAATTY 3 cut(s) 177, 212, 450
Asp718I GGTACC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 549
BanI GGYRCC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 313, 372
BccI CCATC 1 cut(s) 275
BciT130I CCWGG 2 cut(s) 58, 365
BclI TGATCA 1 cut(s) 331
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 2 cut(s) 338, 459
BisI GCNGC 2 cut(s) 302, 361
BlsI GCNGC 2 cut(s) 303, 362
Bme1390I CCNGG 2 cut(s) 58, 365
Bme18I GGWCC 1 cut(s) 549
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 549
BmiI GGNNCC 2 cut(s) 44, 260
BmrFI CCNGG 2 cut(s) 58, 365
BmsI GCATC 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 140
Bpu10I CCTNAGC 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 486
BsaWI WCCGGW 1 cut(s) 551
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 3 cut(s) 66, 123, 434
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 2 cut(s) 58, 365
BseCI ATCGAT 1 cut(s) 486
BseGI GGATG 3 cut(s) 373, 382, 475
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 3 cut(s) 66, 123, 434
BseXI GCAGC 2 cut(s) 313, 372
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 1 cut(s) 537
BshNI GGYRCC 1 cut(s) 42
BshVI ATCGAT 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 490
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 118
BsnI GGCC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 93, 151, 331, 483, 487
BspACI CCGC 1 cut(s) 478
BspANI GGCC 1 cut(s) 537
BspCNI CTCAG 1 cut(s) 571
BspDI ATCGAT 1 cut(s) 486
BspLI GGNNCC 2 cut(s) 44, 260
BspT107I GGYRCC 1 cut(s) 42
BsrBI CCGCTC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 559
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 66, 123, 434
BssMI GATC 5 cut(s) 93, 151, 331, 483, 487
Bst2UI CCWGG 2 cut(s) 58, 365
Bst4CI ACNGT 1 cut(s) 446
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 5 cut(s) 24, 276, 299, 539, 566
BstDEI CTNAG 2 cut(s) 579, 590
BstF5I GGATG 3 cut(s) 373, 382, 475
BstKTI GATC 5 cut(s) 96, 154, 334, 486, 490
BstMBI GATC 5 cut(s) 93, 151, 331, 483, 487
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstNI CCWGG 2 cut(s) 58, 365
BstSCI CCNGG 2 cut(s) 56, 363
BstSFI CTRYAG 2 cut(s) 338, 459
BstV1I GCAGC 2 cut(s) 313, 372
Bsu15I ATCGAT 1 cut(s) 486
BsuRI GGCC 1 cut(s) 537
BsuTUI ATCGAT 1 cut(s) 486
BtsCI GGATG 3 cut(s) 373, 382, 475
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 4 cut(s) 73, 116, 260, 451
Cac8I GCNNGC 5 cut(s) 24, 276, 299, 539, 566
CaiI CAGNNNCTG 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 549
ClaI ATCGAT 1 cut(s) 486
Csp6I GTAC 2 cut(s) 4, 43
CviAII CATG 1 cut(s) 542
CviJI RGCY 9 cut(s) 73, 160, 274, 297, 304, 337, 363, 537, 589
CviKI_1 RGCY 9 cut(s) 73, 160, 274, 297, 304, 337, 363, 537, 589
CviQI GTAC 2 cut(s) 4, 43
DdeI CTNAG 2 cut(s) 579, 590
DpnI GATC 5 cut(s) 95, 153, 333, 485, 489
DpnII GATC 5 cut(s) 93, 151, 331, 483, 487
DraI TTTAAA 1 cut(s) 496
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 56, 363
EcoRV GATATC 1 cut(s) 127
FaeI CATG 1 cut(s) 545
FaiI YATR 2 cut(s) 17, 543
FatI CATG 1 cut(s) 541
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 302, 361
FokI GGATG 3 cut(s) 380, 389, 482
Fsp4HI GCNGC 2 cut(s) 302, 361
FspBI CTAG 1 cut(s) 230
GluI GCNGC 2 cut(s) 302, 361
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 1 cut(s) 537
HapII CCGG 1 cut(s) 552
Hin1II CATG 1 cut(s) 545
HinfI GANTC 3 cut(s) 50, 242, 527
HpaII CCGG 1 cut(s) 552
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 7 cut(s) 13, 85, 131, 151, 309, 325, 580
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 3 cut(s) 156, 465, 577
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4V TGCA 3 cut(s) 116, 541, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
HpyF3I CTNAG 2 cut(s) 579, 590
Hsp92II CATG 1 cut(s) 545
KpnI GGTACC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 5 cut(s) 93, 151, 331, 483, 487
Lsp1109I GCAGC 2 cut(s) 313, 372
LweI GCATC 1 cut(s) 35
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 440
MalI GATC 5 cut(s) 95, 153, 333, 485, 489
MbiI CCGCTC 1 cut(s) 478
MboI GATC 5 cut(s) 93, 151, 331, 483, 487
MboII GAAGA 2 cut(s) 134, 416
MluCI AATT 6 cut(s) 103, 177, 212, 314, 450, 513
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 3 cut(s) 193, 319, 367
MseI TTAA 2 cut(s) 207, 495
MspI CCGG 1 cut(s) 552
MspR9I CCNGG 2 cut(s) 58, 365
MvaI CCWGG 2 cut(s) 58, 365
MwoI GCNNNNNNNGC 1 cut(s) 565
NdeII GATC 5 cut(s) 93, 151, 331, 483, 487
NlaIII CATG 1 cut(s) 545
NlaIV GGNNCC 2 cut(s) 44, 260
NmuCI GTSAC 1 cut(s) 440
PfeI GAWTC 3 cut(s) 50, 242, 527
PkrI GCNGC 2 cut(s) 303, 362
Ple19I CGATCG 1 cut(s) 490
Psp6I CCWGG 2 cut(s) 56, 363
PspGI CCWGG 2 cut(s) 56, 363
PspN4I GGNNCC 2 cut(s) 44, 260
PspPI GGNCC 1 cut(s) 549
PstNI CAGNNNCTG 1 cut(s) 307
PvuI CGATCG 1 cut(s) 490
RsaI GTAC 2 cut(s) 5, 44
RsaNI GTAC 2 cut(s) 4, 43
SaqAI TTAA 2 cut(s) 207, 495
SatI GCNGC 2 cut(s) 302, 361
Sau3AI GATC 5 cut(s) 93, 151, 331, 483, 487
Sau96I GGNCC 1 cut(s) 549
ScrFI CCNGG 2 cut(s) 58, 365
SfaNI GCATC 1 cut(s) 35
SfcI CTRYAG 2 cut(s) 338, 459
SinI GGWCC 1 cut(s) 549
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 6 cut(s) 103, 177, 212, 314, 450, 513
SsiI CCGC 1 cut(s) 478
SspI AATATT 1 cut(s) 599
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 56, 363
TaaI ACNGT 1 cut(s) 446
TaqI TCGA 3 cut(s) 96, 474, 486
TasI AATT 6 cut(s) 103, 177, 212, 314, 450, 513
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 3 cut(s) 50, 242, 527
Tru1I TTAA 2 cut(s) 207, 495
Tru9I TTAA 2 cut(s) 207, 495
TscAI CASTG 4 cut(s) 73, 123, 260, 451
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 301, 360
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 3 cut(s) 225, 393, 525
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 4 cut(s) 73, 123, 260, 451
VpaK11BI GGWCC 1 cut(s) 549
XapI RAATTY 3 cut(s) 177, 212, 450
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.