MD09G1291800.v1.1

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008041
Physical Location & Seq
Forward (+)
37431748 .. 37433136
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1291800.v1.1.491

Sequence Viewer

Length: 897 bp
ATGAAAATGCAAGCTCCAAGTCCTAGTAGTTACACAACTCTGCTCCATATCCCTTGTTCCACTGCTGCAACTGCAACTGCAACTCCCACTCCAAAAGCATTGCTGCTGGTACCACAAAAAAGCCCTAACCTTGCAGCGACAGCGACTGATTTTTCTTTTTCTATTTTTAGAAATCCAAACCTTTTCACCGCCGTCTCTCAATTCAGTAGCAGTCCTCATGTGCAGGTGCAGTGCTCATCTTCAAACAAGGTCGATTCGTCCATCTTGTCCCAGGATGATGACGATGACGATGCTACAGAATCCGTAGCCATATGCGAAGATATTGATCTTGATATAGATATAGAAATAGAGATAGAGAAGACGGGAAACAACTGTCGGAGGATCCGGTTGGAAATTGGGATAGAAGCCCCACTCAACACCGTCTGGAATCTATTGACTGATTACGAGAGATTGGCCGACTTCATTCCGGGACTCGCCGTCTGCCAAGTGCTCGATAAGACGGACGGCTATGCTCGGCTTTTGCAGATTGGGGAGCAGAAGTTGGCGTTGGGGCTGAAATTCAATGCTAAAGGGATCGTAGATTGTTACGAGACTCCACTTGAGACCCTTCCTAATAATTTCGGTCACAAGCGTGATATCCGATTTAACATGGTTGAAGGCGACTTCGAAATCTTTCAAGGAAAATGGTCTCTCCAACAGAAACACCCACACCCGCAGCAGCAGCAGCAAGTGCAAGTGCACACAAGTCTCTCCTACATGGTTGATGTAAAGCCAAAGATGTGGCTGCCCGTTCGCCTTGTTGAGGGTAGACTTTGTAAGGAGATCAAACTAAACCTTGCATGCATCCGACAAGAAGCACTCAAACTAACTCACCACAACACTACTGCTCGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc

Protein Analysis

299

Amino Acids

33.35

Weight (kDa)

5.63

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 134 - 279 2.6e-18 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
Acc36I ACCTGC 1 cut(s) 214
Acc65I GGTACC 1 cut(s) 109
AccB1I GGYRCC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 808
AciI CCGC 2 cut(s) 189, 713
AclWI GGATC 3 cut(s) 376, 389, 581
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 1 cut(s) 557
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 802
AgsI TTSAA 4 cut(s) 243, 562, 656, 677
AhdI GACNNNNNGTC 1 cut(s) 476
AjnI CCWGG 1 cut(s) 270
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AleI CACNNNNGTG 1 cut(s) 630
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw21I GWGCWC 3 cut(s) 236, 492, 741
Alw26I GTCTC 5 cut(s) 199, 584, 596, 693, 752
Alw44I GTGCAC 1 cut(s) 737
AlwI GGATC 3 cut(s) 376, 389, 581
AlwNI CAGNNNCTG 1 cut(s) 146
AoxI GGCC 1 cut(s) 453
ApaLI GTGCAC 1 cut(s) 737
ApeKI GCWGC 8 cut(s) 65, 103, 134, 715, 718, 721, 724, 784
ApoI RAATTY 1 cut(s) 557
Asp700I GAANNNNTTC 1 cut(s) 672
Asp718I GGTACC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 2 cut(s) 178, 863
AsuII TTCGAA 1 cut(s) 666
BaeGI GKGCMC 1 cut(s) 741
BamHI GGATCC 1 cut(s) 381
BanI GGYRCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 3 cut(s) 236, 492, 741
BbvI GCAGC 8 cut(s) 52, 90, 146, 727, 730, 733, 736, 771
BccI CCATC 1 cut(s) 269
BceAI ACGGC 3 cut(s) 176, 461, 520
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 5 cut(s) 199, 584, 596, 693, 752
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 8 cut(s) 66, 104, 135, 716, 719, 722, 725, 785
BlsI GCNGC 8 cut(s) 67, 105, 136, 717, 720, 723, 726, 786
Bme1390I CCNGG 2 cut(s) 272, 468
BmeRI GACNNNNNGTC 1 cut(s) 476
BmiI GGNNCC 2 cut(s) 111, 383
BmrFI CCNGG 2 cut(s) 272, 468
BmsI GCATC 2 cut(s) 280, 852
BpiI GAAGAC 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 324
BsaI GGTCTC 2 cut(s) 596, 693
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 384
BsaXI ACNNNNNCTCC 6 cut(s) 67, 73, 97, 103, 524, 554
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse3DI GCAATG 1 cut(s) 98
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 2 cut(s) 280, 843
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMI GCAATG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 8 cut(s) 52, 90, 146, 727, 730, 733, 736, 771
BsgI GTGCAG 2 cut(s) 242, 248
BshFI GGCC 1 cut(s) 455
BshNI GGYRCC 1 cut(s) 109
BsiHKAI GWGCWC 3 cut(s) 236, 492, 741
BsiSI CCGG 2 cut(s) 385, 467
BslFI GGGAC 2 cut(s) 253, 483
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 5 cut(s) 199, 584, 596, 693, 752
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 2 cut(s) 253, 483
BsnI GGCC 1 cut(s) 455
Bso31I GGTCTC 2 cut(s) 596, 693
Bsp119I TTCGAA 1 cut(s) 666
Bsp1286I GDGCHC 3 cut(s) 236, 492, 741
Bsp143I GATC 4 cut(s) 325, 381, 573, 822
BspACI CCGC 2 cut(s) 189, 713
BspANI GGCC 1 cut(s) 455
BspLI GGNNCC 2 cut(s) 111, 383
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 3 cut(s) 376, 389, 581
BspT104I TTCGAA 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 109
BspTNI GGTCTC 2 cut(s) 596, 693
BsrDI GCAATG 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 4 cut(s) 325, 381, 573, 822
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 2 cut(s) 374, 421
BstAPI GCANNNNNTGC 1 cut(s) 730
BstBI TTCGAA 1 cut(s) 666
BstC8I GCNNGC 2 cut(s) 12, 841
BstENI CCTNNNNNAGG 1 cut(s) 800
BstF5I GGATG 2 cut(s) 280, 843
BstKTI GATC 4 cut(s) 328, 384, 576, 825
BstMAI GTCTC 5 cut(s) 199, 584, 596, 693, 752
BstMBI GATC 4 cut(s) 325, 381, 573, 822
BstMWI GCNNNNNNNGC 5 cut(s) 71, 140, 721, 724, 730
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 843
BstSCI CCNGG 2 cut(s) 270, 466
BstSFI CTRYAG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 741
BstV1I GCAGC 8 cut(s) 52, 90, 146, 727, 730, 733, 736, 771
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 381
BstXI CCANNNNNNTGG 1 cut(s) 780
BstYI RGATCY 1 cut(s) 381
BsuRI GGCC 1 cut(s) 455
BtsCI GGATG 2 cut(s) 280, 843
BtsI GCAGTG 2 cut(s) 60, 236
BtsIMutI CAGTG 2 cut(s) 60, 236
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 12, 841
CaiI CAGNNNCTG 1 cut(s) 146
Csp6I GTAC 1 cut(s) 110
CviAII CATG 4 cut(s) 218, 649, 757, 840
CviQI GTAC 1 cut(s) 110
DpnI GATC 4 cut(s) 327, 383, 575, 824
DpnII GATC 4 cut(s) 325, 381, 573, 822
DriI GACNNNNNGTC 1 cut(s) 476
EaeI YGGCCR 1 cut(s) 453
Eam1105I GACNNNNNGTC 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 596, 693
Eco32I GATATC 1 cut(s) 637
EcoNI CCTNNNNNAGG 1 cut(s) 800
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 637
EcoT22I ATGCAT 1 cut(s) 845
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 4 cut(s) 221, 652, 760, 843
FaqI GGGAC 2 cut(s) 253, 483
FatI CATG 4 cut(s) 217, 648, 756, 839
FauI CCCGC 1 cut(s) 720
FauNDI CATATG 1 cut(s) 311
FblI GTMKAC 1 cut(s) 808
Fnu4HI GCNGC 8 cut(s) 66, 104, 135, 716, 719, 722, 725, 785
FokI GGATG 2 cut(s) 287, 830
Fsp4HI GCNGC 8 cut(s) 66, 104, 135, 716, 719, 722, 725, 785
FspBI CTAG 1 cut(s) 24
GluI GCNGC 8 cut(s) 66, 104, 135, 716, 719, 722, 725, 785
HaeIII GGCC 1 cut(s) 455
HapII CCGG 2 cut(s) 385, 467
Hin1II CATG 4 cut(s) 221, 652, 760, 843
HinfI GANTC 5 cut(s) 254, 299, 427, 471, 592
HpaII CCGG 2 cut(s) 385, 467
HphI GGTGA 2 cut(s) 178, 863
Hpy166II GTNNAC 2 cut(s) 739, 809
Hpy188I TCNGA 3 cut(s) 378, 641, 848
Hpy188III TCNNGA 2 cut(s) 329, 424
Hpy8I GTNNAC 2 cut(s) 739, 809
HpyAV CCTTC 2 cut(s) 617, 650
HpyCH4III ACNGT 2 cut(s) 374, 421
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 140, 721, 724, 730
Hsp92II CATG 4 cut(s) 221, 652, 760, 843
KpnI GGTACC 1 cut(s) 113
Kzo9I GATC 4 cut(s) 325, 381, 573, 822
LmnI GCTCC 3 cut(s) 19, 48, 532
LpnPI CCDG 7 cut(s) 92, 209, 257, 284, 398, 409, 480
Lsp1109I GCAGC 8 cut(s) 52, 90, 146, 727, 730, 733, 736, 771
LweI GCATC 2 cut(s) 280, 852
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 3 cut(s) 29, 584, 623
MalI GATC 4 cut(s) 327, 383, 575, 824
MboI GATC 4 cut(s) 325, 381, 573, 822
MboII GAAGA 3 cut(s) 231, 329, 370
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 3 cut(s) 236, 492, 741
MluCI AATT 4 cut(s) 200, 393, 557, 616
MlyI GAGTC 2 cut(s) 465, 586
MmeI TCCRAC 4 cut(s) 356, 369, 718, 871
MnlI CCTC 3 cut(s) 225, 372, 796
Mph1103I ATGCAT 1 cut(s) 845
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 1 cut(s) 645
MslI CAYNNNNRTG 1 cut(s) 630
MspI CCGG 2 cut(s) 385, 467
MspR9I CCNGG 2 cut(s) 272, 468
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 5 cut(s) 71, 140, 721, 724, 730
NciI CCSGG 1 cut(s) 468
NdeI CATATG 1 cut(s) 311
NdeII GATC 4 cut(s) 325, 381, 573, 822
NlaIII CATG 4 cut(s) 221, 652, 760, 843
NlaIV GGNNCC 2 cut(s) 111, 383
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 623
NsiI ATGCAT 1 cut(s) 845
NspI RCATGY 1 cut(s) 843
NspV TTCGAA 1 cut(s) 666
OliI CACNNNNGTG 1 cut(s) 630
PaeI GCATGC 1 cut(s) 843
PaqCI CACCTGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 3 cut(s) 254, 299, 427
PfoI TCCNGGA 1 cut(s) 466
PkrI GCNGC 8 cut(s) 67, 105, 136, 717, 720, 723, 726, 786
PleI GAGTC 2 cut(s) 465, 586
PpsI GAGTC 2 cut(s) 465, 586
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 111, 383
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 1 cut(s) 381
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 8 cut(s) 66, 104, 135, 716, 719, 722, 725, 785
Sau3AI GATC 4 cut(s) 325, 381, 573, 822
SchI GAGTC 2 cut(s) 465, 586
ScrFI CCNGG 2 cut(s) 272, 468
SduI GDGCHC 3 cut(s) 236, 492, 741
SetI ASST 6 cut(s) 16, 132, 183, 228, 252, 837
SfaNI GCATC 2 cut(s) 280, 852
SfcI CTRYAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SphI GCATGC 1 cut(s) 843
Sse9I AATT 4 cut(s) 200, 393, 557, 616
SsiI CCGC 2 cut(s) 189, 713
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 270, 466
TaaI ACNGT 2 cut(s) 374, 421
TaqI TCGA 3 cut(s) 252, 492, 666
TaqII GACCGA 1 cut(s) 611
TasI AATT 4 cut(s) 200, 393, 557, 616
TfiI GAWTC 3 cut(s) 254, 299, 427
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 2 cut(s) 67, 236
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 8 cut(s) 65, 103, 134, 715, 718, 721, 724, 784
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 2 cut(s) 17, 451
TspGWI ACGGA 2 cut(s) 292, 515
TspRI CASTG 2 cut(s) 67, 236
VneI GTGCAC 1 cut(s) 737
XagI CCTNNNNNAGG 1 cut(s) 800
XapI RAATTY 1 cut(s) 557
XceI RCATGY 1 cut(s) 843
XmiI GTMKAC 1 cut(s) 808
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.