Rh3DG299000

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0008041
Physical Location & Seq
Reverse (-)
31387997 .. 31390045
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG299000.1

Sequence Viewer

Length: 765 bp
ATGTCCTTGAAATCAATCTGCTTCTCTGTTTGGCTCCCTAGCCCTTCTCCGAGCACCGCTGCTCCTGCTTCAATCTCTTCCCCCTATTCCTTTCCTTTGAGAAATCCAAACCACTCCGTCTCCAATTCCGGTTCTTCAAGGCCGCACCTGCTGCATTTCTCGACCAAGCTCGACTCCGCGTTCTTGTCTGAGGATGACGATTACGATGACGATTCCGATTCAGAAGCAGAGAAATCGGAAGTTGATATAGATGTAGATATAGAGTTAGAGATAGAGAAGACGGGGGCCAACTGCCGGAGAATCCGATCGGAAATCCGAATCGAGGCGCCGCTCGCCACCGTCTGGAACTTGTTGACGGATTACGAGAGACTGGCCGACTTCATTCCCGGACTCGCCGTCTGCCAGTTGCTCCAGAAGTCGGAGAACTACGCTCGGCTTTTTCAGATTGGGGAGCAGGACTTGGCGTTTGGGCTGAAATTCAACGCGAAAGGAGTGGTGGATTGTTTTGAGAATCCTCTAGAGAGCATTGCTAATGATTTGGGGGGTCTCACTCTGAAGCGTGATATTGAGTTTAACATGGTCGAAGGTGACTTTGAAATCTTTCGAGGAAAGTGGTCTCTCCAACAGGTACATACACTTCATTTTCTAGTCTTGCTTTACTGTATCTTGATACATTGTCCCAAATCAATATGCTATATACTTGGTAAAACTGGCTCCTCCTTACCAATTGCATGCAACATAGTTGGTGACTTAGGGTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc

Protein Analysis

254

Amino Acids

28.19

Weight (kDa)

4.79

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 107 - 210 2.6e-14 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 325
AccB7I CCANNNNNTGG 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 331
AccII CGCG 2 cut(s) 179, 485
AciI CCGC 4 cut(s) 57, 143, 177, 329
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 575
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 1 cut(s) 630
AfiI CCNNNNNNNGG 4 cut(s) 294, 322, 342, 418
AgsI TTSAA 5 cut(s) 10, 72, 138, 481, 596
AhdI GACNNNNNGTC 2 cut(s) 395, 754
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 4 cut(s) 124, 361, 551, 621
AoxI GGCC 3 cut(s) 140, 285, 372
ApeKI GCWGC 2 cut(s) 59, 151
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 2 cut(s) 285, 756
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 2 cut(s) 599, 758
AvaII GGWCC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 325
BarI GAAGNNNNNNTAC 2 cut(s) 621, 653
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 46, 138
BceAI ACGGC 1 cut(s) 380
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 4 cut(s) 124, 361, 551, 621
BfaI CTAG 3 cut(s) 39, 518, 647
BfoI RGCGCY 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 156
BisI GCNGC 4 cut(s) 60, 143, 152, 329
BlsI GCNGC 4 cut(s) 61, 144, 153, 330
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 756
BmeRI GACNNNNNGTC 2 cut(s) 395, 754
BmgT120I GGNCC 2 cut(s) 285, 756
BmiI GGNNCC 5 cut(s) 35, 286, 327, 715, 757
BmrFI CCNGG 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 2 cut(s) 551, 621
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 104, 134
Bsc4I CCNNNNNNNGG 4 cut(s) 294, 322, 342, 418
Bse1I ACTGG 3 cut(s) 375, 403, 715
Bse3DI GCAATG 1 cut(s) 525
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 4 cut(s) 294, 322, 342, 418
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 3 cut(s) 375, 403, 715
BseRI GAGGAG 1 cut(s) 706
BseXI GCAGC 2 cut(s) 46, 138
Bsh1236I CGCG 2 cut(s) 179, 485
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 3 cut(s) 142, 287, 374
BshNI GGYRCC 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 3 cut(s) 129, 295, 387
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 4 cut(s) 294, 322, 342, 418
BsmAI GTCTC 4 cut(s) 124, 361, 551, 621
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 3 cut(s) 142, 287, 374
Bso31I GGTCTC 2 cut(s) 551, 621
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 1 cut(s) 305
BspACI CCGC 4 cut(s) 57, 143, 177, 329
BspANI GGCC 3 cut(s) 142, 287, 374
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 2 cut(s) 179, 485
BspLI GGNNCC 5 cut(s) 35, 286, 327, 715, 757
BspMI ACCTGC 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 325
BspTNI GGTCTC 2 cut(s) 551, 621
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 525
BsrI ACTGG 3 cut(s) 375, 403, 715
BssMI GATC 1 cut(s) 305
BssNI GRCGYC 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 340, 662
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 326
BstAPI GCANNNNNTGC 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 333, 733
BstDEI CTNAG 2 cut(s) 189, 751
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 2 cut(s) 179, 485
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 1 cut(s) 308
BstMAI GTCTC 4 cut(s) 124, 361, 551, 621
BstMBI GATC 1 cut(s) 305
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 4 cut(s) 65, 148, 151, 332
BstNSI RCATGY 1 cut(s) 735
BstSCI CCNGG 1 cut(s) 385
BstUI CGCG 2 cut(s) 179, 485
BstV1I GCAGC 2 cut(s) 46, 138
BstV2I GAAGAC 1 cut(s) 284
BsuRI GGCC 3 cut(s) 142, 287, 374
BtsCI GGATG 1 cut(s) 199
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 333, 733
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 2 cut(s) 285, 756
Csp6I GTAC 1 cut(s) 629
CviAII CATG 2 cut(s) 577, 732
CviJI RGCY 9 cut(s) 34, 42, 142, 169, 287, 374, 436, 472, 714
CviKI_1 RGCY 9 cut(s) 34, 42, 142, 169, 287, 374, 436, 472, 714
CviQI GTAC 1 cut(s) 629
DdeI CTNAG 2 cut(s) 189, 751
DinI GGCGCC 1 cut(s) 327
DpnI GATC 1 cut(s) 307
DpnII GATC 1 cut(s) 305
DriI GACNNNNNGTC 2 cut(s) 395, 754
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 82
Eam1105I GACNNNNNGTC 2 cut(s) 395, 754
EarI CTCTTC 1 cut(s) 82
Eco31I GGTCTC 2 cut(s) 551, 621
Eco47I GGWCC 1 cut(s) 756
Eco57I CTGAAG 1 cut(s) 575
EgeI GGCGCC 1 cut(s) 327
EheI GGCGCC 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 2 cut(s) 580, 735
FaiI YATR 9 cut(s) 248, 260, 578, 633, 691, 696, 698, 733, 740
FaqI GGGAC 1 cut(s) 663
FatI CATG 2 cut(s) 576, 731
Fnu4HI GCNGC 4 cut(s) 60, 143, 152, 329
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 4 cut(s) 60, 143, 152, 329
FspBI CTAG 3 cut(s) 39, 518, 647
GlaI GCGC 1 cut(s) 327
GluI GCNGC 4 cut(s) 60, 143, 152, 329
GsuI CTGGAG 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 3 cut(s) 142, 287, 374
HapII CCGG 3 cut(s) 129, 295, 387
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 2 cut(s) 580, 735
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 7 cut(s) 173, 212, 218, 300, 318, 390, 511
HpaII CCGG 3 cut(s) 129, 295, 387
HphI GGTGA 2 cut(s) 599, 758
Hpy166II GTNNAC 1 cut(s) 354
Hpy188III TCNNGA 5 cut(s) 160, 343, 412, 518, 667
Hpy8I GTNNAC 1 cut(s) 354
HpyAV CCTTC 2 cut(s) 54, 578
HpyCH4III ACNGT 2 cut(s) 340, 662
HpyCH4V TGCA 3 cut(s) 154, 731, 735
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 148, 151, 332
HpyF3I CTNAG 2 cut(s) 189, 751
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 2 cut(s) 580, 735
HspAI GCGC 1 cut(s) 326
KasI GGCGCC 1 cut(s) 325
Kzo9I GATC 1 cut(s) 305
LmnI GCTCC 5 cut(s) 39, 67, 414, 451, 719
Lsp1109I GCAGC 2 cut(s) 46, 138
MaeI CTAG 3 cut(s) 39, 518, 647
MaeIII GTNAC 2 cut(s) 587, 746
MalI GATC 1 cut(s) 307
MbiI CCGCTC 1 cut(s) 331
MboI GATC 1 cut(s) 305
MboII GAAGA 3 cut(s) 69, 126, 289
MfeI CAATTG 1 cut(s) 726
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 3 cut(s) 124, 476, 726
Mly113I GGCGCC 1 cut(s) 326
MlyI GAGTC 2 cut(s) 167, 384
MmeI TCCRAC 2 cut(s) 399, 646
MnlI CCTC 5 cut(s) 184, 316, 525, 599, 727
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 3 cut(s) 129, 295, 387
MspR9I CCNGG 1 cut(s) 387
MunI CAATTG 1 cut(s) 726
MvnI CGCG 2 cut(s) 179, 485
MwoI GCNNNNNNNGC 4 cut(s) 65, 148, 151, 332
NarI GGCGCC 1 cut(s) 326
NciI CCSGG 1 cut(s) 387
NdeII GATC 1 cut(s) 305
NlaIII CATG 2 cut(s) 580, 735
NlaIV GGNNCC 5 cut(s) 35, 286, 327, 715, 757
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 2 cut(s) 587, 746
NspI RCATGY 1 cut(s) 735
PaeI GCATGC 1 cut(s) 735
PaqCI CACCTGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 5 cut(s) 212, 218, 300, 318, 511
PflMI CCANNNNNTGG 1 cut(s) 342
PfoI TCCNGGA 1 cut(s) 385
PkrI GCNGC 4 cut(s) 61, 144, 153, 330
Ple19I CGATCG 1 cut(s) 308
PleI GAGTC 2 cut(s) 167, 384
PluTI GGCGCC 1 cut(s) 329
PpsI GAGTC 2 cut(s) 167, 384
PspN4I GGNNCC 5 cut(s) 35, 286, 327, 715, 757
PspPI GGNCC 2 cut(s) 285, 756
PvuI CGATCG 1 cut(s) 308
RsaI GTAC 1 cut(s) 630
RsaNI GTAC 1 cut(s) 629
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 4 cut(s) 60, 143, 152, 329
Sau3AI GATC 1 cut(s) 305
Sau96I GGNCC 2 cut(s) 285, 756
SchI GAGTC 2 cut(s) 167, 384
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 56
SetI ASST 4 cut(s) 150, 171, 589, 630
SfoI GGCGCC 1 cut(s) 327
SinI GGWCC 1 cut(s) 756
SphI GCATGC 1 cut(s) 735
Sse9I AATT 3 cut(s) 124, 476, 726
SsiI CCGC 4 cut(s) 57, 143, 177, 329
SspDI GGCGCC 1 cut(s) 325
SspMI CTAG 3 cut(s) 39, 518, 647
StyD4I CCNGG 1 cut(s) 385
TaaI ACNGT 2 cut(s) 340, 662
TaqI TCGA 5 cut(s) 161, 171, 321, 582, 604
TasI AATT 3 cut(s) 124, 476, 726
TauI GCSGC 2 cut(s) 145, 331
TfiI GAWTC 5 cut(s) 212, 218, 300, 318, 511
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 2 cut(s) 587, 746
TseI GCWGC 2 cut(s) 59, 151
Tsp45I GTSAC 2 cut(s) 587, 746
TspDTI ATGAA 2 cut(s) 370, 629
TspGWI ACGGA 2 cut(s) 106, 371
Van91I CCANNNNNTGG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 756
XapI RAATTY 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 517
XceI RCATGY 1 cut(s) 735
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 3 cut(s) 39, 518, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.