Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002947

Physical Location & Seq

Genomic Coordinates Forward (+)

5393343 .. 5397057

Gene Structure 3715 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1041500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGTGACGGAGGACGATTCAGCCGCGCTTTTCTGTGACGGCCACTTTGCCGCGCCGGGGTTTCGGTTCCATCCCACCGACGAGGAGCTTGTCCTCTACTATCTGAAGCGGAAGATCTGTAAGAAGAGGCTCAAGATTGACGTCATCGCCGAGACCGACGTCTACAAGTGGGACCCTGACGAACTTCCTGAACTATCTCTGTTGAAAACTGGAGACAGGCAATGGTTCTTTTTCAGTCCGAGAGATCGGAAATACCCTAATGGAGGAAGATCAAGCAGGGCAACCAGACACGGGTATTGGAAAGCTACAGGAAAGGATCGTACCATTGTATGCTATTCACGGTCTGTTGGATTGAAGAAGACCTTAGTTTATTACAAAGGCCGTGCACCTAGTGGGGAGCGCACCGACTGGGTGATGCATGAGTATACCTTGGATGAGGAAGAGCTCAAGAGATGCCAGAATGTACAGCAATACTATGCTCTCTACAAGGTTTACAAAAAAAGTGGACCTGGTCCCAAAAATGGCGAGCAATATGGGGCACCATTTAGGGAAGAAGACTGGGTTGATGATGAATCCACAGTAATCAAGTCTGCTGCTGATCACCAAATCTCAGTGAAGCAAGCTGTAGATGTTGTTTCTATTGATAATGTGAAAGTCAATGGGGATGTCCATTATGCACTAAATGATATCGAGGAGTTCATGAAACAGATTGCTGATGAGGCTGTGCTGCCTGAAATGAATGGGTACGGTTACACAGCACCCCAGGTTCTTAGCGAAGAAGATACCCAAAGTACTCTGGTTGATCTGTACTCCAGGGAAGTTATCTGTGCAGAACCTGTCAGAGAGTTCAATCCAAATGTGCAGCAACGCAATGTGGATGCTAACTTTGACTTCACGCAGTCATCTGCCTCCCAAATGCAATCTTACGAGGCATCTGAAGTCACCACAGCTGCTCCTGACATTTATGACCAGGACCCTCCTGCACTGCATGAAGAGGATTTCTTGGAGATGGATGATCTCCTTGGTCCTGAACCTACTGTACCAAACACTGTGAACCCTGTAGATGACTTGCAGTTTGGAGAGCTAGATGGATTAAGTGAATTCGACTTTTATCATGATGCAGCCATGTTTCTTCATGACTTGGGGCCTATTGATGAAGGAAATGTTGCTCATCATCAGTACATGAATTCTCAGGGGAATAATATAGTAGACCAGTTTGAATACCATTTACAACCCAATCCAGTAGCTGAAAACCAGGTTAACCATCTGCTGAATCCAGAATCAACTCAGATTAACAATCAGATGTGGACAAACACTGAAAGAGCAGAACCAAATCAGGGATCTCTCTCGTATTCAACCCCAGGTGTGGTATATGAATCTTCAAATTTTCCTTCCCAAGCAACTCAAAATCAAAGTGGCAATGAGGCTGCAGGTGCTACAAGTCAGTTCTCTTCTGCTCTTTGGGCCTTTGTGGAGTCCATACCTACCACCCCTGCATCGGCTTCAGAGAATGCTTTGGTGAATCGTGCTTTTGAACGGATGTCTAGCTTCAGCAGGTTGAGAATTAATACGGCAAGTACAACAGTGGCTGCAGGTAATGATTCTGAAACTAGGAGGGCAGGGAGAAAGAGGGGATTCTTCTTCCTTCCGGTTCTTGTAGCACTATGTGCCGTTTTTTGGGTTTTGATTGCAACTCTCAGACCATGGGGGAGATCCCTCTCCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

576

Amino Acids

64.84

Weight (kDa)

4.77

Isoelectric Point (pI)

41.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	19 - 144	7.7e-46	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002947)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G34180 AT1G34180 AT1G34180 AT1G34180 AT1G34190
fragaria_vesca	FvH4_2g12690 FvH4_2g12690 FvH4_2g12690 FvH4_2g12690 FvH4_2g12690 FvH4_6g07970
malus_domestica	MD05G1037700.v1.1 MD10G1041500.v1.1
prunus_persica	Prupe.8G045800_v2.0.a1 Prupe.8G045800_v2.0.a1
pyrus_communis	pycom05g02790 pycom10g02860
rosa_chinensis	RchiOBHm_Chr3g0457681 RchiOBHm_Chr6g0273431
rosa_laevigata	RLG00000013619 RLG00000025196
rosa_multiflora	Rmu_co8212274.1_g000001 Rmu_sc0005891.1_g000016 Rmu_sc0007395.1_g000006
rosa_roxburghii	Rroxscaffold_6G00421580 Rroxscaffold_7G00195380
rosa_rugosa	Rorug03G0022500 Rorug06G0074700
rosa_samantha	Rh3AG083200 Rh3BG085700 Rh3CG086000 Rh3DG086600 Rh6AG189800 Rh6BG193300 Rh6CG193000 Rh6DG184500
rosa_wichuraiana	Rw3G007120 Rw6G016520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1421
AatII	GACGTC	2 cut(s)	144, 162
Acc36I	ACCTGC	3 cut(s)	1421, 1545, 1583
AccB1I	GGYRCC	1 cut(s)	538
AccI	GTMKAC	3 cut(s)	162, 425, 1209
AccII	CGCG	2 cut(s)	26, 53
AciI	CCGC	3 cut(s)	24, 51, 109
AclWI	GGATC	3 cut(s)	324, 1348, 1707
AcoI	YGGCCR	1 cut(s)	40
AcsI	RAATTY	3 cut(s)	1100, 1186, 1384
AcuI	CTGAAG	4 cut(s)	125, 957, 1488, 1534
AcyI	GRCGYC	2 cut(s)	141, 159
AdeI	CACNNNGTG	2 cut(s)	392, 1667
AfaI	GTAC	8 cut(s)	322, 465, 746, 793, 809, 1041, 1181, 1579
AfiI	CCNNNNNNNGG	8 cut(s)	57, 263, 291, 521, 1337, 1366, 1498, 1677
AgsI	TTSAA	7 cut(s)	205, 355, 850, 1220, 1356, 1383, 1535
AjnI	CCWGG	6 cut(s)	508, 762, 812, 969, 1254, 1360
AluBI	AGCT	8 cut(s)	88, 305, 445, 623, 950, 1084, 1247, 1548
AluI	AGCT	8 cut(s)	88, 305, 445, 623, 950, 1084, 1247, 1548
Alw21I	GWGCWC	2 cut(s)	388, 447
Alw26I	GTCTC	2 cut(s)	146, 207
Alw44I	GTGCAC	1 cut(s)	384
AlwI	GGATC	3 cut(s)	324, 1348, 1707
AlwNI	CAGNNNCTG	3 cut(s)	836, 1247, 1589
AoxI	GGCC	4 cut(s)	40, 379, 1145, 1464
ApaLI	GTGCAC	1 cut(s)	384
ApeKI	GCWGC	7 cut(s)	593, 727, 862, 950, 1121, 1427, 1589
ApoI	RAATTY	3 cut(s)	1100, 1186, 1384
ArsI	GACNNNNNNTTYG	4 cut(s)	29, 61, 1058, 1090
AseI	ATTAAT	1 cut(s)	1566
AspLEI	GCGC	3 cut(s)	28, 55, 402
AspS9I	GGNCC	7 cut(s)	172, 506, 512, 973, 1025, 1145, 1464
AsuC2I	CCSGG	1 cut(s)	57
AsuHPI	GGTGA	5 cut(s)	16, 424, 593, 934, 1531
AvaII	GGWCC	5 cut(s)	172, 506, 512, 973, 1025
BaeGI	GKGCMC	2 cut(s)	388, 541
BanI	GGYRCC	1 cut(s)	538
BanII	GRGCYC	1 cut(s)	447
BbsI	GAAGAC	2 cut(s)	365, 561
Bbv12I	GWGCWC	2 cut(s)	388, 447
BbvI	GCAGC	7 cut(s)	580, 714, 874, 937, 1133, 1414, 1576
BccI	CCATC	4 cut(s)	78, 1003, 1082, 1272
BceAI	ACGGC	4 cut(s)	55, 366, 1587, 1655
BciT130I	CCWGG	6 cut(s)	510, 764, 814, 971, 1256, 1362
BclI	TGATCA	1 cut(s)	598
BcnI	CCSGG	1 cut(s)	57
BcoDI	GTCTC	2 cut(s)	146, 207
BfaI	CTAG	4 cut(s)	390, 1085, 1545, 1611
BfmI	CTRYAG	5 cut(s)	306, 624, 1059, 1428, 1590
BfuAI	ACCTGC	3 cut(s)	1421, 1545, 1583
BglII	AGATCT	1 cut(s)	114
BisI	GCNGC	9 cut(s)	24, 51, 594, 728, 863, 951, 1122, 1428, 1590
BlsI	GCNGC	9 cut(s)	25, 52, 595, 729, 864, 952, 1123, 1429, 1591
BmcAI	AGTACT	1 cut(s)	793
Bme1390I	CCNGG	7 cut(s)	57, 510, 764, 814, 971, 1256, 1362
Bme18I	GGWCC	5 cut(s)	172, 506, 512, 973, 1025
BmgT120I	GGNCC	7 cut(s)	172, 506, 512, 973, 1025, 1145, 1464
BmiI	GGNNCC	7 cut(s)	68, 173, 174, 514, 540, 975, 1146
BmrFI	CCNGG	7 cut(s)	57, 510, 764, 814, 971, 1256, 1362
BmrI	ACTGGG	2 cut(s)	418, 568
BmsI	GCATC	6 cut(s)	405, 443, 868, 941, 1108, 1505
BmuI	ACTGGG	2 cut(s)	418, 568
BoxI	GACNNNNGTC	1 cut(s)	158
BpiI	GAAGAC	2 cut(s)	365, 561
BpmI	CTGGAG	2 cut(s)	231, 796
BpuEI	CTTGAG	2 cut(s)	116, 431
BpuMI	CCSGG	1 cut(s)	57
BsaHI	GRCGYC	2 cut(s)	141, 159
BsaI	GGTCTC	1 cut(s)	146
BsaJI	CCNNGG	7 cut(s)	56, 429, 762, 813, 1021, 1360, 1703
BsaWI	WCCGGW	1 cut(s)	1648
BsaXI	ACNNNNNCTCC	2 cut(s)	937, 967
Bsc4I	CCNNNNNNNGG	8 cut(s)	57, 263, 291, 521, 1337, 1366, 1498, 1677
Bse1I	ACTGG	5 cut(s)	214, 413, 563, 1213, 1241
Bse3DI	GCAATG	3 cut(s)	227, 877, 1426
BseBI	CCWGG	6 cut(s)	510, 764, 814, 971, 1256, 1362
BseDI	CCNNGG	7 cut(s)	56, 429, 762, 813, 1021, 1360, 1703
BseGI	GGATG	6 cut(s)	70, 439, 670, 883, 1018, 1545
BseLI	CCNNNNNNNGG	8 cut(s)	57, 263, 291, 521, 1337, 1366, 1498, 1677
BseMI	GCAATG	3 cut(s)	227, 877, 1426
BseMII	CTCAG	4 cut(s)	624, 1205, 1301, 1711
BseNI	ACTGG	5 cut(s)	214, 413, 563, 1213, 1241
BseRI	GAGGAG	2 cut(s)	98, 707
BseSI	GKGCMC	2 cut(s)	388, 541
BseXI	GCAGC	7 cut(s)	580, 714, 874, 937, 1133, 1414, 1576
BsgI	GTGCAG	3 cut(s)	849, 881, 966
Bsh1236I	CGCG	2 cut(s)	26, 53
BshFI	GGCC	4 cut(s)	42, 381, 1147, 1466
BshNI	GGYRCC	1 cut(s)	538
BsiHKAI	GWGCWC	2 cut(s)	388, 447
BsiSI	CCGG	2 cut(s)	56, 1649
BslFI	GGGAC	2 cut(s)	185, 498
BslI	CCNNNNNNNGG	8 cut(s)	57, 263, 291, 521, 1337, 1366, 1498, 1677
BsmAI	GTCTC	2 cut(s)	146, 207
BsmFI	GGGAC	2 cut(s)	185, 498
BsmI	GAATGC	1 cut(s)	1516
BsnI	GGCC	4 cut(s)	42, 381, 1147, 1466
Bso31I	GGTCTC	1 cut(s)	146
Bsp1286I	GDGCHC	3 cut(s)	388, 447, 541
Bsp1407I	TGTACA	1 cut(s)	463
Bsp143I	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
Bsp19I	CCATGG	1 cut(s)	1703
BspACI	CCGC	3 cut(s)	24, 51, 109
BspANI	GGCC	4 cut(s)	42, 381, 1147, 1466
BspCNI	CTCAG	4 cut(s)	623, 1204, 1300, 1710
BspFNI	CGCG	2 cut(s)	26, 53
BspHI	TCATGA	3 cut(s)	699, 1114, 1135
BspLI	GGNNCC	7 cut(s)	68, 173, 174, 514, 540, 975, 1146
BspMAI	CTGCAG	2 cut(s)	1432, 1594
BspMI	ACCTGC	3 cut(s)	1421, 1545, 1583
BspPI	GGATC	3 cut(s)	324, 1348, 1707
BspQI	GCTCTTC	1 cut(s)	435
BspT107I	GGYRCC	1 cut(s)	538
BspTNI	GGTCTC	1 cut(s)	146
BsrDI	GCAATG	3 cut(s)	227, 877, 1426
BsrGI	TGTACA	1 cut(s)	463
BsrI	ACTGG	5 cut(s)	214, 413, 563, 1213, 1241
BssECI	CCNNGG	7 cut(s)	56, 429, 762, 813, 1021, 1360, 1703
BssMI	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
BssNAI	GTATAC	1 cut(s)	426
BssNI	GRCGYC	2 cut(s)	141, 159
BssT1I	CCWWGG	3 cut(s)	429, 1021, 1703
Bst1107I	GTATAC	1 cut(s)	426
Bst2UI	CCWGG	6 cut(s)	510, 764, 814, 971, 1256, 1362
Bst4CI	ACNGT	6 cut(s)	342, 580, 749, 1039, 1051, 1585
Bst6I	CTCTTC	4 cut(s)	119, 435, 987, 1456
BstACI	GRCGYC	2 cut(s)	141, 159
BstAPI	GCANNNNNTGC	1 cut(s)	1667
BstAUI	TGTACA	1 cut(s)	463
BstC8I	GCNNGC	2 cut(s)	527, 621
BstDEI	CTNAG	6 cut(s)	364, 610, 770, 1191, 1287, 1697
BstDSI	CCRYGG	1 cut(s)	1703
BstENI	CCTNNNNNAGG	1 cut(s)	261
BstF5I	GGATG	6 cut(s)	70, 439, 670, 883, 1018, 1545
BstFNI	CGCG	2 cut(s)	26, 53
BstHHI	GCGC	3 cut(s)	28, 55, 402
BstKTI	GATC	9 cut(s)	117, 247, 272, 319, 601, 805, 1018, 1343, 1715
BstMAI	GTCTC	2 cut(s)	146, 207
BstMBI	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
BstMWI	GCNNNNNNNGC	4 cut(s)	719, 1433, 1463, 1667
BstNI	CCWGG	6 cut(s)	510, 764, 814, 971, 1256, 1362
BstPAI	GACNNNNGTC	1 cut(s)	158
BstSCI	CCNGG	7 cut(s)	55, 508, 762, 812, 969, 1254, 1360
BstSFI	CTRYAG	5 cut(s)	306, 624, 1059, 1428, 1590
BstSLI	GKGCMC	2 cut(s)	388, 541
BstUI	CGCG	2 cut(s)	26, 53
BstV1I	GCAGC	7 cut(s)	580, 714, 874, 937, 1133, 1414, 1576
BstV2I	GAAGAC	2 cut(s)	365, 561
BstX2I	RGATCY	3 cut(s)	114, 1340, 1712
BstYI	RGATCY	3 cut(s)	114, 1340, 1712
BstZ17I	GTATAC	1 cut(s)	426
BsuRI	GGCC	4 cut(s)	42, 381, 1147, 1466
BtgI	CCRYGG	1 cut(s)	1703
BtgZI	GCGATG	1 cut(s)	130
BtsCI	GGATG	6 cut(s)	70, 439, 670, 883, 1018, 1545
BtsI	GCAGTG	1 cut(s)	983
BtsIMutI	CAGTG	5 cut(s)	618, 983, 1047, 1314, 1590
BveI	ACCTGC	3 cut(s)	1421, 1545, 1583
Cac8I	GCNNGC	2 cut(s)	527, 621
CaiI	CAGNNNCTG	3 cut(s)	836, 1247, 1589
CciI	TCATGA	3 cut(s)	699, 1114, 1135
CfoI	GCGC	3 cut(s)	28, 55, 402
Cfr13I	GGNCC	7 cut(s)	172, 506, 512, 973, 1025, 1145, 1464
CsiI	ACCWGGT	2 cut(s)	508, 1254
Csp6I	GTAC	8 cut(s)	321, 464, 745, 792, 808, 1040, 1180, 1578
CspCI	CAANNNNNGTGG	2 cut(s)	484, 519
CviAII	CATG	8 cut(s)	419, 700, 989, 1115, 1126, 1136, 1183, 1704
CviQI	GTAC	8 cut(s)	321, 464, 745, 792, 808, 1040, 1180, 1578
DdeI	CTNAG	6 cut(s)	364, 610, 770, 1191, 1287, 1697
DpnI	GATC	9 cut(s)	116, 246, 271, 318, 600, 804, 1017, 1342, 1714
DpnII	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
DraIII	CACNNNGTG	2 cut(s)	392, 1667
EaeI	YGGCCR	1 cut(s)	40
Eam1104I	CTCTTC	4 cut(s)	119, 435, 987, 1456
EarI	CTCTTC	4 cut(s)	119, 435, 987, 1456
Ecl136II	GAGCTC	1 cut(s)	445
Eco130I	CCWWGG	3 cut(s)	429, 1021, 1703
Eco24I	GRGCYC	1 cut(s)	447
Eco31I	GGTCTC	1 cut(s)	146
Eco32I	GATATC	1 cut(s)	688
Eco47I	GGWCC	5 cut(s)	172, 506, 512, 973, 1025
Eco53kI	GAGCTC	1 cut(s)	445
Eco57I	CTGAAG	4 cut(s)	125, 957, 1488, 1534
EcoICRI	GAGCTC	1 cut(s)	445
EcoNI	CCTNNNNNAGG	1 cut(s)	261
EcoO109I	RGGNCCY	3 cut(s)	172, 973, 1145
EcoRI	GAATTC	2 cut(s)	1100, 1186
EcoRII	CCWGG	6 cut(s)	508, 762, 812, 969, 1254, 1360
EcoRV	GATATC	1 cut(s)	688
EcoT14I	CCWWGG	3 cut(s)	429, 1021, 1703
EcoT22I	ATGCAT	1 cut(s)	420
EcoT38I	GRGCYC	1 cut(s)	447
ErhI	CCWWGG	3 cut(s)	429, 1021, 1703
FaeI	CATG	8 cut(s)	422, 703, 992, 1118, 1129, 1139, 1186, 1707
FalI	AAGNNNNNCTT	2 cut(s)	347, 379
FaqI	GGGAC	2 cut(s)	185, 498
FatI	CATG	8 cut(s)	418, 699, 988, 1114, 1125, 1135, 1182, 1703
FbaI	TGATCA	1 cut(s)	598
FblI	GTMKAC	3 cut(s)	162, 425, 1209
Fnu4HI	GCNGC	9 cut(s)	24, 51, 594, 728, 863, 951, 1122, 1428, 1590
FokI	GGATG	6 cut(s)	57, 446, 677, 890, 1025, 1552
FriOI	GRGCYC	1 cut(s)	447
Fsp4HI	GCNGC	9 cut(s)	24, 51, 594, 728, 863, 951, 1122, 1428, 1590
FspBI	CTAG	4 cut(s)	390, 1085, 1545, 1611
GlaI	GCGC	3 cut(s)	27, 54, 401
GluI	GCNGC	9 cut(s)	24, 51, 594, 728, 863, 951, 1122, 1428, 1590
GsuI	CTGGAG	2 cut(s)	231, 796
HaeIII	GGCC	4 cut(s)	42, 381, 1147, 1466
HapII	CCGG	2 cut(s)	56, 1649
HhaI	GCGC	3 cut(s)	28, 55, 402
Hin1I	GRCGYC	2 cut(s)	141, 159
Hin1II	CATG	8 cut(s)	422, 703, 992, 1118, 1129, 1139, 1186, 1707
Hin6I	GCGC	3 cut(s)	26, 53, 400
HinP1I	GCGC	3 cut(s)	26, 53, 400
HincII	GTYRAC	1 cut(s)	1261
HindII	GTYRAC	1 cut(s)	1261
HinfI	GANTC	9 cut(s)	17, 572, 1273, 1280, 1376, 1475, 1522, 1601, 1635
HpaI	GTTAAC	1 cut(s)	1261
HpaII	CCGG	2 cut(s)	56, 1649
HphI	GGTGA	5 cut(s)	16, 424, 593, 934, 1531
Hpy166II	GTNNAC	9 cut(s)	163, 386, 426, 493, 506, 1054, 1210, 1261, 1308
Hpy188III	TCNNGA	9 cut(s)	133, 188, 448, 700, 956, 1028, 1115, 1136, 1277
Hpy8I	GTNNAC	9 cut(s)	163, 386, 426, 493, 506, 1054, 1210, 1261, 1308
Hpy99I	CGWCG	2 cut(s)	83, 161
HpyAV	CCTTC	3 cut(s)	1151, 1401, 1655
HpyCH4III	ACNGT	6 cut(s)	342, 580, 749, 1039, 1051, 1585
HpyCH4IV	ACGT	2 cut(s)	141, 159
HpyF10VI	GCNNNNNNNGC	4 cut(s)	719, 1433, 1463, 1667
HpyF3I	CTNAG	6 cut(s)	364, 610, 770, 1191, 1287, 1697
HpySE526I	ACGT	2 cut(s)	141, 159
Hsp92I	GRCGYC	2 cut(s)	141, 159
Hsp92II	CATG	8 cut(s)	422, 703, 992, 1118, 1129, 1139, 1186, 1707
HspAI	GCGC	3 cut(s)	26, 53, 400
KflI	GGGWCCC	1 cut(s)	172
Ksp22I	TGATCA	1 cut(s)	598
KspAI	GTTAAC	1 cut(s)	1261
Kzo9I	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
LguI	GCTCTTC	1 cut(s)	435
LmnI	GCTCC	3 cut(s)	85, 397, 958
Lsp1109I	GCAGC	7 cut(s)	580, 714, 874, 937, 1133, 1414, 1576
LweI	GCATC	6 cut(s)	405, 443, 868, 941, 1108, 1505
MabI	ACCWGGT	2 cut(s)	508, 1254
MaeI	CTAG	4 cut(s)	390, 1085, 1545, 1611
MaeII	ACGT	2 cut(s)	141, 159
MaeIII	GTNAC	4 cut(s)	4, 35, 749, 940
MalI	GATC	9 cut(s)	116, 246, 271, 318, 600, 804, 1017, 1342, 1714
MboI	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
MflI	RGATCY	3 cut(s)	114, 1340, 1712
MhlI	GDGCHC	3 cut(s)	388, 447, 541
MluCI	AATT	4 cut(s)	1100, 1186, 1384, 1563
MlyI	GAGTC	1 cut(s)	1484
MmeI	TCCRAC	1 cut(s)	328
Mph1103I	ATGCAT	1 cut(s)	420
MseI	TTAA	4 cut(s)	1094, 1260, 1293, 1566
MspA1I	CMGCKG	1 cut(s)	950
MspI	CCGG	2 cut(s)	56, 1649
MspR9I	CCNGG	7 cut(s)	57, 510, 764, 814, 971, 1256, 1362
Mva1269I	GAATGC	1 cut(s)	1516
MvaI	CCWGG	6 cut(s)	510, 764, 814, 971, 1256, 1362
MvnI	CGCG	2 cut(s)	26, 53
MwoI	GCNNNNNNNGC	4 cut(s)	719, 1433, 1463, 1667
NciI	CCSGG	1 cut(s)	57
NcoI	CCATGG	1 cut(s)	1703
NdeII	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
NlaIII	CATG	8 cut(s)	422, 703, 992, 1118, 1129, 1139, 1186, 1707
NlaIV	GGNNCC	7 cut(s)	68, 173, 174, 514, 540, 975, 1146
NmeAIII	GCCGAG	1 cut(s)	175
NmuCI	GTSAC	3 cut(s)	4, 35, 940
NsiI	ATGCAT	1 cut(s)	420
PagI	TCATGA	3 cut(s)	699, 1114, 1135
PaqCI	CACCTGC	1 cut(s)	1421
PciSI	GCTCTTC	1 cut(s)	435
PcsI	WCGNNNNNNNCGW	2 cut(s)	147, 153
PctI	GAATGC	1 cut(s)	1516
PfeI	GAWTC	8 cut(s)	17, 572, 1273, 1280, 1376, 1522, 1601, 1635
PflFI	GACNNNGTC	1 cut(s)	510
PkrI	GCNGC	9 cut(s)	25, 52, 595, 729, 864, 952, 1123, 1429, 1591
PleI	GAGTC	1 cut(s)	1483
PpsI	GAGTC	1 cut(s)	1483
PpuMI	RGGWCCY	2 cut(s)	172, 973
PshAI	GACNNNNGTC	1 cut(s)	158
PshBI	ATTAAT	1 cut(s)	1566
Psp124BI	GAGCTC	1 cut(s)	447
Psp5II	RGGWCCY	2 cut(s)	172, 973
Psp6I	CCWGG	6 cut(s)	508, 762, 812, 969, 1254, 1360
PspGI	CCWGG	6 cut(s)	508, 762, 812, 969, 1254, 1360
PspN4I	GGNNCC	7 cut(s)	68, 173, 174, 514, 540, 975, 1146
PspPI	GGNCC	7 cut(s)	172, 506, 512, 973, 1025, 1145, 1464
PspPPI	RGGWCCY	2 cut(s)	172, 973
PsrI	GAACNNNNNNTAC	2 cut(s)	1023, 1055
PstI	CTGCAG	2 cut(s)	1432, 1594
PstNI	CAGNNNCTG	3 cut(s)	836, 1247, 1589
PsuI	RGATCY	3 cut(s)	114, 1340, 1712
PsyI	GACNNNGTC	1 cut(s)	510
PvuII	CAGCTG	1 cut(s)	950
RsaI	GTAC	8 cut(s)	322, 465, 746, 793, 809, 1041, 1181, 1579
RsaNI	GTAC	8 cut(s)	321, 464, 745, 792, 808, 1040, 1180, 1578
SacI	GAGCTC	1 cut(s)	447
SapI	GCTCTTC	1 cut(s)	435
SaqAI	TTAA	4 cut(s)	1094, 1260, 1293, 1566
SatI	GCNGC	9 cut(s)	24, 51, 594, 728, 863, 951, 1122, 1428, 1590
Sau3AI	GATC	9 cut(s)	114, 244, 269, 316, 598, 802, 1015, 1340, 1712
Sau96I	GGNCC	7 cut(s)	172, 506, 512, 973, 1025, 1145, 1464
ScaI	AGTACT	1 cut(s)	793
SchI	GAGTC	1 cut(s)	1484
ScrFI	CCNGG	7 cut(s)	57, 510, 764, 814, 971, 1256, 1362
SduI	GDGCHC	3 cut(s)	388, 447, 541
SexAI	ACCWGGT	2 cut(s)	508, 1254
SfaNI	GCATC	6 cut(s)	405, 443, 868, 941, 1108, 1505
SfcI	CTRYAG	5 cut(s)	306, 624, 1059, 1428, 1590
SinI	GGWCC	5 cut(s)	172, 506, 512, 973, 1025
SmlI	CTYRAG	2 cut(s)	131, 446
SmoI	CTYRAG	2 cut(s)	131, 446
Sse9I	AATT	4 cut(s)	1100, 1186, 1384, 1563
SsiI	CCGC	3 cut(s)	24, 51, 109
SspMI	CTAG	4 cut(s)	390, 1085, 1545, 1611
SstI	GAGCTC	1 cut(s)	447
StyD4I	CCNGG	7 cut(s)	55, 508, 762, 812, 969, 1254, 1360
StyI	CCWWGG	3 cut(s)	429, 1021, 1703
TaaI	ACNGT	6 cut(s)	342, 580, 749, 1039, 1051, 1585
TaiI	ACGT	2 cut(s)	144, 162
TaqI	TCGA	2 cut(s)	690, 1104
TaqII	GACCGA	1 cut(s)	170
TasI	AATT	4 cut(s)	1100, 1186, 1384, 1563
TatI	WGTACW	5 cut(s)	463, 791, 807, 1179, 1577
TauI	GCSGC	2 cut(s)	26, 53
TfiI	GAWTC	8 cut(s)	17, 572, 1273, 1280, 1376, 1522, 1601, 1635
Tru1I	TTAA	4 cut(s)	1094, 1260, 1293, 1566
Tru9I	TTAA	4 cut(s)	1094, 1260, 1293, 1566
TscAI	CASTG	5 cut(s)	618, 990, 1054, 1321, 1590
TseFI	GTSAC	3 cut(s)	4, 35, 940
TseI	GCWGC	7 cut(s)	593, 727, 862, 950, 1121, 1427, 1589
Tsp45I	GTSAC	3 cut(s)	4, 35, 940
TspDTI	ATGAA	9 cut(s)	585, 688, 716, 752, 1005, 1124, 1170, 1199, 1389
TspGWI	ACGGA	2 cut(s)	23, 1552
TspRI	CASTG	5 cut(s)	618, 990, 1054, 1321, 1590
Tth111I	GACNNNGTC	1 cut(s)	510
VneI	GTGCAC	1 cut(s)	384
VpaK11BI	GGWCC	5 cut(s)	172, 506, 512, 973, 1025
VspI	ATTAAT	1 cut(s)	1566
XagI	CCTNNNNNAGG	1 cut(s)	261
XapI	RAATTY	3 cut(s)	1100, 1186, 1384
XmiI	GTMKAC	3 cut(s)	162, 425, 1209
XspI	CTAG	4 cut(s)	390, 1085, 1545, 1611
ZraI	GACGTC	2 cut(s)	142, 160
ZrmI	AGTACT	1 cut(s)	793
Zsp2I	ATGCAT	1 cut(s)	420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.