RchiOBHm_Chr6g0273431
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002947
Physical Location & Seq
Reverse (-)
34318591 .. 34322465
3875 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24530

Sequence Viewer

Length: 1791 bp
ATGGTCTCAGGAGACGACTTGGCGTCCGCCTTCTGCGACGGCCACTTTTGGCAGCCGGGGTTTCGGTTCCATCCCACCGACGAGGAACTCGTCCTCTACTATCTCAAGCGGAAGATCTGCAAGAAGAGGCTCAAGCTTAACGTCATCGCCGAGACCGACGTCTATAAGTGGGACCCTGAGGAACTTCCTGGATTGTCTTTATTGAAAACTGGAGACAGGCAATGGTTCTTTTTCAGTCCGAGAGATCGGAGGTACCCTAATGGGGGGAGATCAAACAGGGCAACCAGACATGGGTATTGGAAAGCTACTGGAAAGGATCGCAACATTTCCTGTTATAACCGATCAGTTGGACTGAAGAAGACCTTAGTTTTCTATAAAGGCCGTGCACCTACTGGGGAGCGCACTGATTGGGTGATGCATGAGTATACCTTGGATGAGGAGGAGTTAAAGAGATGCCAGAATGTACAGGAATATTATGCTCTGTACAAAGTTTTCAAGAAAAGTGGACCTGGTCCTAAAAATGGTGAGCAATATGGGGCGCCATTTAGAGAAGAAGATTGGGCTGATGATGAATGCCCGATAATAAATAATTCTGCTGATAGGGAAATCTCAGTTCAGCAAGTTGGAGAGCTTGCTGCTAATTATAATGTGAAGCCTTTTGGAGAAATTCCATTGAAGCAAGTTGAAGAGATTACTGCTAATGGTTATGTGAATGTTAACAGCGAAGTCCATTCTACAATAGATGACTTGGAACGGTTCATGAAACAGATTGCTGATGATGCTGTGCTTGACCTGCCACAGTTTAATGATCATGTTCACACCCTATCTCAGGTTGTTAGCGAAGAAGAAACACAAAGTACTGTGGTGGATCTGTACTCCAGTGAAGTTAAATGCCCACAATCGATTACAACTTTTGATCCAAGTGGTCAGCAGGGCAATGCACATGCATGCTTTGACTTCACACAGTCAGCTACTTCACAGATGCAACTGTACGAGGCATCTGAAGCATCTGCTTCTGACGTTCATCAACCGGAGCCTTTCACTCCCATACTACGTGAGGAGGACTTCCTGGAGATGGATGATCTCCTGGGTCCTGAACCTACTATTTCAAACACTGGGAATTCCGGGGGTAACTTGCAGTTTGATGAGTTGGATGGATTTGACATGTTCCATGATGCGGCCATGTTTTTCAATGACATGGGGCCCATTGAACAAGGAACTGTTTCTCATCAATTCATGAATTCAATGGAGGGTAATATTGTAAACCAGTTTGATTACCAGATACAACCAGCACCTCCAATTCAGATCAACCATCAGCTGACTCAAGAGTCAACTCAGATTAACAATCAGCTATGGGTACAGAATGAGAGGCAAGATCTCTATACTTCAGCAGAATCAAAGCAGGGGTCTGGACCTCAATTCAACTCAGGTGTGGTATACGATTCGTCAAATCATCCTCCACAAGCAAATCAAAATCAAAGTGGCAATGAAGCTGCCAGTGTTACTAGTCAGTTTTCTTCTGCCCTATGGTCTTTTGTGGAGTCTATACCTACTACTCCTGCATCTGCATCAGAGAATGCCTTGGTGAGTCGGGCCTTTGAACGGATGAATAGCTTTAGCAGGATGAGAATAATTGCGAGGAGCGCAAATGTCCCTGCAGGTAATGATTCTGAAACTAGGGGATCCCGAAAGAGGGGATTCTTCTTCATTCCAGTTCTTGTGGCACTATGTGCTATTTTTTGGGTCTTAATGGCATCTCTCAGGCAATGGGGGAGATGCATCTCCTCATGA

Protein Analysis

596

Amino Acids

67.31

Weight (kDa)

4.9

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 19 - 144 2.9e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 336, 645
AasI GACNNNNNNGTC 1 cut(s) 1327
AatII GACGTC 1 cut(s) 162
Acc36I ACCTGC 2 cut(s) 801, 1649
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 2 cut(s) 252, 538
AccI GTMKAC 2 cut(s) 425, 1437
AciI CCGC 3 cut(s) 27, 109, 1178
AclWI GGATC 5 cut(s) 324, 876, 911, 1677, 1690
AcoI YGGCCR 2 cut(s) 40, 1179
AcsI RAATTY 3 cut(s) 666, 1120, 1240
AcuI CTGAAG 3 cut(s) 374, 1023, 1371
AcyI GRCGYC 3 cut(s) 23, 159, 539
AdeI CACNNNGTG 1 cut(s) 1730
AfaI GTAC 7 cut(s) 254, 465, 485, 859, 875, 992, 1359
AflIII ACRYGT 1 cut(s) 1164
AhdI GACNNNNNGTC 1 cut(s) 22
AhlI ACTAGT 1 cut(s) 1505
AjnI CCWGG 4 cut(s) 187, 508, 1068, 1086
AluBI AGCT 8 cut(s) 136, 305, 631, 971, 1318, 1351, 1493, 1614
AluI AGCT 8 cut(s) 136, 305, 631, 971, 1318, 1351, 1493, 1614
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 4 cut(s) 6, 10, 146, 207
Alw44I GTGCAC 1 cut(s) 384
AlwI GGATC 5 cut(s) 324, 876, 911, 1677, 1690
AoxI GGCC 5 cut(s) 40, 379, 1179, 1202, 1593
ApaI GGGCCC 1 cut(s) 1206
ApaLI GTGCAC 1 cut(s) 384
ApeKI GCWGC 3 cut(s) 52, 635, 1493
ApoI RAATTY 3 cut(s) 666, 1120, 1240
ArsI GACNNNNNNTTYG 2 cut(s) 1391, 1423
Asp700I GAANNNNTTC 1 cut(s) 1222
Asp718I GGTACC 1 cut(s) 252
AspLEI GCGC 3 cut(s) 402, 541, 1646
AspS9I GGNCC 8 cut(s) 172, 506, 512, 1091, 1202, 1203, 1412, 1593
AsuC2I CCSGG 2 cut(s) 57, 1126
AsuHPI GGTGA 3 cut(s) 424, 536, 1597
AvaII GGWCC 5 cut(s) 172, 506, 512, 1091, 1412
AxyI CCTNAGG 1 cut(s) 177
BaeGI GKGCMC 2 cut(s) 388, 1206
BamHI GGATCC 1 cut(s) 1682
BanI GGYRCC 2 cut(s) 252, 538
BanII GRGCYC 1 cut(s) 1206
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 3 cut(s) 64, 622, 1480
BccI CCATC 4 cut(s) 78, 1069, 1148, 1320
BceAI ACGGC 2 cut(s) 55, 366
BciT130I CCWGG 4 cut(s) 189, 510, 1070, 1088
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 2 cut(s) 57, 1126
BcoDI GTCTC 4 cut(s) 6, 10, 146, 207
BcuI ACTAGT 1 cut(s) 1505
BfaI CTAG 2 cut(s) 1506, 1677
BfmI CTRYAG 1 cut(s) 1656
BfoI RGCGCY 1 cut(s) 542
BfuAI ACCTGC 2 cut(s) 801, 1649
BglII AGATCT 2 cut(s) 114, 1375
BisI GCNGC 4 cut(s) 53, 636, 1179, 1494
BlsI GCNGC 4 cut(s) 54, 637, 1180, 1495
BmcAI AGTACT 1 cut(s) 859
Bme1390I CCNGG 6 cut(s) 57, 189, 510, 1070, 1088, 1126
Bme18I GGWCC 5 cut(s) 172, 506, 512, 1091, 1412
BmeRI GACNNNNNGTC 1 cut(s) 22
BmgT120I GGNCC 8 cut(s) 172, 506, 512, 1091, 1202, 1203, 1412, 1593
BmrFI CCNGG 6 cut(s) 57, 189, 510, 1070, 1088, 1126
BmrI ACTGGG 2 cut(s) 402, 1125
BmuI ACTGGG 2 cut(s) 402, 1125
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 365
BpmI CTGGAG 3 cut(s) 231, 862, 1091
BpuEI CTTGAG 3 cut(s) 89, 116, 1308
BpuMI CCSGG 2 cut(s) 57, 1126
Bsa29I ATCGAT 1 cut(s) 902
BsaAI YACGTR 1 cut(s) 1055
BsaHI GRCGYC 3 cut(s) 23, 159, 539
BsaI GGTCTC 2 cut(s) 10, 146
BsaJI CCNNGG 5 cut(s) 56, 429, 1087, 1125, 1581
BsaWI WCCGGW 1 cut(s) 1030
Bse1I ACTGG 8 cut(s) 214, 313, 397, 879, 1120, 1267, 1497, 1712
Bse21I CCTNAGG 1 cut(s) 177
Bse3DI GCAATG 4 cut(s) 227, 943, 1492, 1772
BseBI CCWGG 4 cut(s) 189, 510, 1070, 1088
BseCI ATCGAT 1 cut(s) 902
BseDI CCNNGG 5 cut(s) 56, 429, 1087, 1125, 1581
BseGI GGATG 7 cut(s) 70, 439, 1084, 1159, 1453, 1611, 1629
BseMI GCAATG 4 cut(s) 227, 943, 1492, 1772
BseMII CTCAG 7 cut(s) 21, 168, 624, 842, 1349, 1440, 1774
BseNI ACTGG 8 cut(s) 214, 313, 397, 879, 1120, 1267, 1497, 1712
BseRI GAGGAG 5 cut(s) 452, 455, 1073, 1654, 1774
BseSI GKGCMC 2 cut(s) 388, 1206
BseXI GCAGC 3 cut(s) 64, 622, 1480
BshFI GGCC 5 cut(s) 42, 381, 1181, 1204, 1595
BshNI GGYRCC 2 cut(s) 252, 538
BshVI ATCGAT 1 cut(s) 902
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 3 cut(s) 56, 1031, 1125
BslFI GGGAC 2 cut(s) 185, 1637
BsmAI GTCTC 4 cut(s) 6, 10, 146, 207
BsmBI CGTCTC 1 cut(s) 6
BsmFI GGGAC 2 cut(s) 185, 1637
BsmI GAATGC 2 cut(s) 578, 1582
BsnI GGCC 5 cut(s) 42, 381, 1181, 1204, 1595
Bso31I GGTCTC 2 cut(s) 10, 146
Bsp120I GGGCCC 1 cut(s) 1202
Bsp1286I GDGCHC 2 cut(s) 388, 1206
Bsp1407I TGTACA 2 cut(s) 463, 483
BspACI CCGC 3 cut(s) 27, 109, 1178
BspANI GGCC 5 cut(s) 42, 381, 1181, 1204, 1595
BspCNI CTCAG 7 cut(s) 20, 169, 623, 841, 1348, 1439, 1773
BspDI ATCGAT 1 cut(s) 902
BspHI TCATGA 3 cut(s) 759, 1236, 1787
BspMAI CTGCAG 1 cut(s) 1660
BspMI ACCTGC 2 cut(s) 801, 1649
BspPI GGATC 5 cut(s) 324, 876, 911, 1677, 1690
BspT107I GGYRCC 2 cut(s) 252, 538
BspTNI GGTCTC 2 cut(s) 10, 146
BsrDI GCAATG 4 cut(s) 227, 943, 1492, 1772
BsrGI TGTACA 2 cut(s) 463, 483
BsrI ACTGG 8 cut(s) 214, 313, 397, 879, 1120, 1267, 1497, 1712
BssECI CCNNGG 5 cut(s) 56, 429, 1087, 1125, 1581
BssNAI GTATAC 2 cut(s) 426, 1438
BssNI GRCGYC 3 cut(s) 23, 159, 539
BssT1I CCWWGG 2 cut(s) 429, 1581
Bst1107I GTATAC 2 cut(s) 426, 1438
Bst2UI CCWGG 4 cut(s) 189, 510, 1070, 1088
Bst4CI ACNGT 6 cut(s) 756, 801, 862, 966, 990, 1222
Bst6I CTCTTC 2 cut(s) 119, 681
BstACI GRCGYC 3 cut(s) 23, 159, 539
BstAPI GCANNNNNTGC 1 cut(s) 1730
BstAUI TGTACA 2 cut(s) 463, 483
BstBAI YACGTR 1 cut(s) 1055
BstC8I GCNNGC 2 cut(s) 633, 949
BstDEI CTNAG 8 cut(s) 7, 177, 364, 610, 828, 1335, 1426, 1760
BstENI CCTNNNNNAGG 1 cut(s) 827
BstF5I GGATG 7 cut(s) 70, 439, 1084, 1159, 1453, 1611, 1629
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 3 cut(s) 402, 541, 1646
BstMAI GTCTC 4 cut(s) 6, 10, 146, 207
BstMWI GCNNNNNNNGC 5 cut(s) 779, 793, 1004, 1643, 1730
BstNI CCWGG 4 cut(s) 189, 510, 1070, 1088
BstNSI RCATGY 3 cut(s) 947, 951, 1168
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 6 cut(s) 55, 187, 508, 1068, 1086, 1124
BstSFI CTRYAG 1 cut(s) 1656
BstSLI GKGCMC 2 cut(s) 388, 1206
BstV1I GCAGC 3 cut(s) 64, 622, 1480
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 4 cut(s) 114, 868, 1375, 1682
BstYI RGATCY 4 cut(s) 114, 868, 1375, 1682
BstZ17I GTATAC 2 cut(s) 426, 1438
Bsu15I ATCGAT 1 cut(s) 902
Bsu36I CCTNAGG 1 cut(s) 177
BsuRI GGCC 5 cut(s) 42, 381, 1181, 1204, 1595
BsuTUI ATCGAT 1 cut(s) 902
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 7 cut(s) 70, 439, 1084, 1159, 1453, 1611, 1629
BtsIMutI CAGTG 4 cut(s) 402, 886, 1113, 1504
BveI ACCTGC 2 cut(s) 801, 1649
Cac8I GCNNGC 2 cut(s) 633, 949
CciI TCATGA 3 cut(s) 759, 1236, 1787
CfoI GCGC 3 cut(s) 402, 541, 1646
Cfr13I GGNCC 8 cut(s) 172, 506, 512, 1091, 1202, 1203, 1412, 1593
ClaI ATCGAT 1 cut(s) 902
CseI GACGC 1 cut(s) 12
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 7 cut(s) 253, 464, 484, 858, 874, 991, 1358
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CviQI GTAC 7 cut(s) 253, 464, 484, 858, 874, 991, 1358
DdeI CTNAG 8 cut(s) 7, 177, 364, 610, 828, 1335, 1426, 1760
DinI GGCGCC 1 cut(s) 540
DraIII CACNNNGTG 1 cut(s) 1730
DrdI GACNNNNNNGTC 1 cut(s) 1327
DriI GACNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 1327
EaeI YGGCCR 2 cut(s) 40, 1179
Eam1104I CTCTTC 2 cut(s) 119, 681
Eam1105I GACNNNNNGTC 1 cut(s) 22
EarI CTCTTC 2 cut(s) 119, 681
EciI GGCGGA 1 cut(s) 16
Eco130I CCWWGG 2 cut(s) 429, 1581
Eco24I GRGCYC 1 cut(s) 1206
Eco31I GGTCTC 2 cut(s) 10, 146
Eco47I GGWCC 5 cut(s) 172, 506, 512, 1091, 1412
Eco57I CTGAAG 3 cut(s) 374, 1023, 1371
Eco81I CCTNAGG 1 cut(s) 177
EcoNI CCTNNNNNAGG 1 cut(s) 827
EcoO109I RGGNCCY 3 cut(s) 172, 1091, 1202
EcoRI GAATTC 2 cut(s) 1120, 1240
EcoRII CCWGG 4 cut(s) 187, 508, 1068, 1086
EcoT14I CCWWGG 2 cut(s) 429, 1581
EcoT22I ATGCAT 3 cut(s) 420, 949, 1781
EcoT38I GRGCYC 1 cut(s) 1206
EgeI GGCGCC 1 cut(s) 540
EheI GGCGCC 1 cut(s) 540
ErhI CCWWGG 2 cut(s) 429, 1581
Esp3I CGTCTC 1 cut(s) 6
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 2 cut(s) 185, 1637
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 2 cut(s) 425, 1437
Fnu4HI GCNGC 4 cut(s) 53, 636, 1179, 1494
FokI GGATG 7 cut(s) 57, 446, 1091, 1166, 1440, 1618, 1636
FriOI GRGCYC 1 cut(s) 1206
Fsp4HI GCNGC 4 cut(s) 53, 636, 1179, 1494
FspBI CTAG 2 cut(s) 1506, 1677
GlaI GCGC 3 cut(s) 401, 540, 1645
GluI GCNGC 4 cut(s) 53, 636, 1179, 1494
GsuI CTGGAG 3 cut(s) 231, 862, 1091
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 5 cut(s) 42, 381, 1181, 1204, 1595
HapII CCGG 3 cut(s) 56, 1031, 1125
HgaI GACGC 1 cut(s) 12
HhaI GCGC 3 cut(s) 402, 541, 1646
Hin1I GRCGYC 3 cut(s) 23, 159, 539
Hin6I GCGC 3 cut(s) 400, 539, 1644
HinP1I GCGC 3 cut(s) 400, 539, 1644
HincII GTYRAC 2 cut(s) 718, 1332
HindII GTYRAC 2 cut(s) 718, 1332
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 8 cut(s) 1321, 1328, 1394, 1442, 1541, 1588, 1667, 1698
HpaI GTTAAC 1 cut(s) 718
HpaII CCGG 3 cut(s) 56, 1031, 1125
HphI GGTGA 3 cut(s) 424, 536, 1597
Hpy166II GTNNAC 8 cut(s) 386, 426, 506, 718, 817, 1264, 1332, 1438
Hpy188I TCNGA 8 cut(s) 240, 249, 1003, 1018, 1305, 1338, 1573, 1672
Hpy188III TCNNGA 9 cut(s) 9, 496, 760, 1094, 1237, 1325, 1410, 1686, 1788
Hpy8I GTNNAC 8 cut(s) 386, 426, 506, 718, 817, 1264, 1332, 1438
Hpy99I CGWCG 3 cut(s) 41, 83, 161
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 6 cut(s) 756, 801, 862, 966, 990, 1222
HpyCH4IV ACGT 4 cut(s) 141, 159, 1020, 1054
HpyF10VI GCNNNNNNNGC 5 cut(s) 779, 793, 1004, 1643, 1730
HpyF3I CTNAG 8 cut(s) 7, 177, 364, 610, 828, 1335, 1426, 1760
HpySE526I ACGT 4 cut(s) 141, 159, 1020, 1054
Hsp92I GRCGYC 3 cut(s) 23, 159, 539
HspAI GCGC 3 cut(s) 400, 539, 1644
KasI GGCGCC 1 cut(s) 538
KflI GGGWCCC 1 cut(s) 172
KpnI GGTACC 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 808
KspAI GTTAAC 1 cut(s) 718
LmnI GCTCC 3 cut(s) 397, 1033, 1641
Lsp1109I GCAGC 3 cut(s) 64, 622, 1480
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 2 cut(s) 1506, 1677
MaeII ACGT 4 cut(s) 141, 159, 1020, 1054
MaeIII GTNAC 2 cut(s) 1130, 1501
MflI RGATCY 4 cut(s) 114, 868, 1375, 1682
MhlI GDGCHC 2 cut(s) 388, 1206
MluCI AATT 9 cut(s) 589, 640, 666, 1120, 1232, 1240, 1299, 1418, 1632
Mly113I GGCGCC 1 cut(s) 539
MlyI GAGTC 4 cut(s) 1315, 1337, 1550, 1597
MmeI TCCRAC 3 cut(s) 328, 604, 1131
Mph1103I ATGCAT 3 cut(s) 420, 949, 1781
MroXI GAANNNNTTC 1 cut(s) 1222
MseI TTAA 7 cut(s) 138, 446, 717, 804, 888, 1341, 1748
MslI CAYNNNNRTG 1 cut(s) 946
MspA1I CMGCKG 1 cut(s) 1318
MspI CCGG 3 cut(s) 56, 1031, 1125
MspR9I CCNGG 6 cut(s) 57, 189, 510, 1070, 1088, 1126
Mva1269I GAATGC 2 cut(s) 578, 1582
MvaI CCWGG 4 cut(s) 189, 510, 1070, 1088
MwoI GCNNNNNNNGC 5 cut(s) 779, 793, 1004, 1643, 1730
NarI GGCGCC 1 cut(s) 539
NciI CCSGG 2 cut(s) 57, 1126
NmeAIII GCCGAG 1 cut(s) 175
NsiI ATGCAT 3 cut(s) 420, 949, 1781
NspI RCATGY 3 cut(s) 947, 951, 1168
PaeI GCATGC 1 cut(s) 951
PagI TCATGA 3 cut(s) 759, 1236, 1787
PciI ACATGT 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 147, 153
PctI GAATGC 2 cut(s) 578, 1582
PdmI GAANNNNTTC 1 cut(s) 1222
PfeI GAWTC 4 cut(s) 1394, 1442, 1667, 1698
PflFI GACNNNGTC 1 cut(s) 510
PfoI TCCNGGA 2 cut(s) 187, 1068
PkrI GCNGC 4 cut(s) 54, 637, 1180, 1495
PleI GAGTC 4 cut(s) 1315, 1336, 1549, 1596
PluTI GGCGCC 1 cut(s) 542
PpsI GAGTC 4 cut(s) 1315, 1336, 1549, 1596
Ppu21I YACGTR 1 cut(s) 1055
PpuMI RGGWCCY 2 cut(s) 172, 1091
PscI ACATGT 1 cut(s) 1164
PshAI GACNNNNGTC 1 cut(s) 158
PsiI TTATAA 2 cut(s) 336, 645
Psp5II RGGWCCY 2 cut(s) 172, 1091
Psp6I CCWGG 4 cut(s) 187, 508, 1068, 1086
PspGI CCWGG 4 cut(s) 187, 508, 1068, 1086
PspOMI GGGCCC 1 cut(s) 1202
PspPI GGNCC 8 cut(s) 172, 506, 512, 1091, 1202, 1203, 1412, 1593
PspPPI RGGWCCY 2 cut(s) 172, 1091
PstI CTGCAG 1 cut(s) 1660
PsuI RGATCY 4 cut(s) 114, 868, 1375, 1682
PsyI GACNNNGTC 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 1318
RsaI GTAC 7 cut(s) 254, 465, 485, 859, 875, 992, 1359
RsaNI GTAC 7 cut(s) 253, 464, 484, 858, 874, 991, 1358
RseI CAYNNNNRTG 1 cut(s) 946
SaqAI TTAA 7 cut(s) 138, 446, 717, 804, 888, 1341, 1748
SatI GCNGC 4 cut(s) 53, 636, 1179, 1494
Sau96I GGNCC 8 cut(s) 172, 506, 512, 1091, 1202, 1203, 1412, 1593
SbfI CCTGCAGG 1 cut(s) 1660
ScaI AGTACT 1 cut(s) 859
SchI GAGTC 4 cut(s) 1315, 1337, 1550, 1597
ScrFI CCNGG 6 cut(s) 57, 189, 510, 1070, 1088, 1126
SdaI CCTGCAGG 1 cut(s) 1660
SduI GDGCHC 2 cut(s) 388, 1206
SexAI ACCWGGT 1 cut(s) 508
SfcI CTRYAG 1 cut(s) 1656
SfoI GGCGCC 1 cut(s) 540
SinI GGWCC 5 cut(s) 172, 506, 512, 1091, 1412
SmiMI CAYNNNNRTG 1 cut(s) 946
SmlI CTYRAG 3 cut(s) 104, 131, 1323
SmoI CTYRAG 3 cut(s) 104, 131, 1323
SpeI ACTAGT 1 cut(s) 1505
SphI GCATGC 1 cut(s) 951
Sse8387I CCTGCAGG 1 cut(s) 1660
Sse9I AATT 9 cut(s) 589, 640, 666, 1120, 1232, 1240, 1299, 1418, 1632
SsiI CCGC 3 cut(s) 27, 109, 1178
SspDI GGCGCC 1 cut(s) 538
SspI AATATT 2 cut(s) 473, 1258
SspMI CTAG 2 cut(s) 1506, 1677
StyD4I CCNGG 6 cut(s) 55, 187, 508, 1068, 1086, 1124
StyI CCWWGG 2 cut(s) 429, 1581
TaaI ACNGT 6 cut(s) 756, 801, 862, 966, 990, 1222
TaiI ACGT 4 cut(s) 144, 162, 1023, 1057
TaqI TCGA 1 cut(s) 902
TaqII GACCGA 1 cut(s) 170
TasI AATT 9 cut(s) 589, 640, 666, 1120, 1232, 1240, 1299, 1418, 1632
TatI WGTACW 4 cut(s) 463, 483, 857, 873
TauI GCSGC 1 cut(s) 1181
TfiI GAWTC 4 cut(s) 1394, 1442, 1667, 1698
Tru1I TTAA 7 cut(s) 138, 446, 717, 804, 888, 1341, 1748
Tru9I TTAA 7 cut(s) 138, 446, 717, 804, 888, 1341, 1748
TscAI CASTG 4 cut(s) 409, 886, 1120, 1504
TseI GCWGC 3 cut(s) 52, 635, 1493
TspDTI ATGAA 9 cut(s) 585, 748, 776, 1013, 1225, 1253, 1503, 1622, 1696
TspGWI ACGGA 1 cut(s) 1618
TspRI CASTG 4 cut(s) 409, 886, 1120, 1504
Tth111I GACNNNGTC 1 cut(s) 510
VneI GTGCAC 1 cut(s) 384
VpaK11BI GGWCC 5 cut(s) 172, 506, 512, 1091, 1412
XagI CCTNNNNNAGG 1 cut(s) 827
XapI RAATTY 3 cut(s) 666, 1120, 1240
XceI RCATGY 3 cut(s) 947, 951, 1168
XmiI GTMKAC 2 cut(s) 425, 1437
XmnI GAANNNNTTC 1 cut(s) 1222
XspI CTAG 2 cut(s) 1506, 1677
ZraI GACGTC 1 cut(s) 160
ZrmI AGTACT 1 cut(s) 859
Zsp2I ATGCAT 3 cut(s) 420, 949, 1781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.