RchiOBHm_Chr3g0457681
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002947
Physical Location & Seq
Forward (+)
6703352 .. 6704069
718 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42443

Sequence Viewer

Length: 678 bp
ATGATGGTATCAGCGGACGACTTGGTGATCGTCTTTCGCCAGCAACCAGGGTTCCGATTCCATCCCACCGAAGAGGAACTCGTCCTCTACTATCTGAAACGAAAAATCTGCCACAAAAGGTTCCAGCTCAACATCATTACTGAAACCGACGTCTACAAGTGCGAGCCGGAGGAGCTTCCCGGTTTGTCTTTATTACAAACCGGAGACCTGCAATGGTTCTTTTTCAACCCTAGAAATTGTAAGTACCCTAACAAACCTACAAGAATAAACAGGGCGACCAGACATGGCTACTGGAAAGTTACAGGAAAAGATCGCACGATTACGAATAATTCACGGTCGGTTGGAGTAAAGAAAACCCTAGTTTTCTATACAGGCCGTGCGCCTAGTGGGAATCGCACCGACTGGGTGATGCATGAGTATACTTTGCACGATGAGGAGCTGAAGAGATGTGGGAATGTACCGGATGCTTATGCTCTTTGCAAGGTTTACAAGAAAAGTGGACTGGGTCCAAAAAACGGTGAGCAATATGGGGCGCCGTTTAAGGAGGAAGACTGCTCTGATATGGATGAAGTCCAAGAAACCCCAGTGAAGCAAATTGAAAATCAGATTAGTAGGGCGAAAGGAGGAGTTAATCCAGTCACTAAAAGTTACAGTTACAGGACATGGAGGAGTTCATGA

Protein Analysis

225

Amino Acids

26.13

Weight (kDa)

9.12

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 16 - 142 1.1e-43 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 532
AccI GTMKAC 2 cut(s) 153, 419
AciI CCGC 1 cut(s) 14
AcuI CTGAAG 1 cut(s) 461
AcyI GRCGYC 2 cut(s) 150, 533
AfaI GTAC 2 cut(s) 245, 459
AfiI CCNNNNNNNGG 1 cut(s) 515
AgsI TTSAA 2 cut(s) 226, 599
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 3 cut(s) 127, 175, 439
AluI AGCT 3 cut(s) 127, 175, 439
Alw26I GTCTC 1 cut(s) 198
AoxI GGCC 1 cut(s) 373
AspLEI GCGC 2 cut(s) 382, 535
AspS9I GGNCC 1 cut(s) 506
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 3 cut(s) 37, 418, 530
AvaII GGWCC 1 cut(s) 506
BanI GGYRCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 555
BccI CCATC 1 cut(s) 69
BceAI ACGGC 2 cut(s) 360, 520
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 1 cut(s) 48
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 198
BfaI CTAG 3 cut(s) 231, 359, 384
BfoI RGCGCY 1 cut(s) 536
BfuAI ACCTGC 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 48, 180
Bme18I GGWCC 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 4 cut(s) 53, 122, 507, 534
BmrFI CCNGG 2 cut(s) 48, 180
BmrI ACTGGG 3 cut(s) 412, 512, 578
BmsI GCATC 2 cut(s) 399, 454
BmuI ACTGGG 3 cut(s) 412, 512, 578
BpiI GAAGAC 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 180
BsaHI GRCGYC 2 cut(s) 150, 533
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 47
BsaWI WCCGGW 2 cut(s) 200, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse1I ACTGG 5 cut(s) 296, 407, 507, 584, 635
Bse3DI GCAATG 1 cut(s) 218
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 3 cut(s) 61, 469, 571
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 218
BseNI ACTGG 5 cut(s) 296, 407, 507, 584, 635
BseRI GAGGAG 3 cut(s) 185, 449, 639
Bsh1285I CGRYCG 1 cut(s) 338
BshFI GGCC 1 cut(s) 375
BshNI GGYRCC 1 cut(s) 532
BsiEI CGRYCG 1 cut(s) 338
BsiSI CCGG 4 cut(s) 167, 180, 201, 461
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 1 cut(s) 198
BsnI GGCC 1 cut(s) 375
Bso31I GGTCTC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 27, 310
BspACI CCGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 375
BspHI TCATGA 1 cut(s) 674
BspLI GGNNCC 4 cut(s) 53, 122, 507, 534
BspMI ACCTGC 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 218
BsrI ACTGG 5 cut(s) 296, 407, 507, 584, 635
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 2 cut(s) 27, 310
BssNAI GTATAC 1 cut(s) 420
BssNI GRCGYC 2 cut(s) 150, 533
Bst1107I GTATAC 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 336, 518, 653
Bst6I CTCTTC 2 cut(s) 66, 437
BstACI GRCGYC 2 cut(s) 150, 533
BstC8I GCNNGC 2 cut(s) 41, 164
BstF5I GGATG 3 cut(s) 61, 469, 571
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 2 cut(s) 382, 535
BstKTI GATC 2 cut(s) 30, 313
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 2 cut(s) 27, 310
BstMCI CGRYCG 1 cut(s) 338
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 1 cut(s) 48
BstSCI CCNGG 2 cut(s) 46, 178
BstV2I GAAGAC 1 cut(s) 555
BstZ17I GTATAC 1 cut(s) 420
BsuRI GGCC 1 cut(s) 375
BtsCI GGATG 3 cut(s) 61, 469, 571
BtsIMutI CAGTG 1 cut(s) 591
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 41, 164
CciI TCATGA 1 cut(s) 674
CfoI GCGC 2 cut(s) 382, 535
Cfr13I GGNCC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 244, 458
CspCI CAANNNNNGTGG 2 cut(s) 478, 513
CviAII CATG 4 cut(s) 284, 413, 663, 675
CviJI RGCY 6 cut(s) 127, 166, 175, 288, 375, 439
CviKI_1 RGCY 6 cut(s) 127, 166, 175, 288, 375, 439
CviQI GTAC 2 cut(s) 244, 458
DinI GGCGCC 1 cut(s) 534
DpnI GATC 2 cut(s) 29, 312
DpnII GATC 2 cut(s) 27, 310
Eam1104I CTCTTC 2 cut(s) 66, 437
EarI CTCTTC 2 cut(s) 66, 437
Eco31I GGTCTC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 506
Eco57I CTGAAG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 46
EcoT22I ATGCAT 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 534
EheI GGCGCC 1 cut(s) 534
FaeI CATG 4 cut(s) 287, 416, 666, 678
FaiI YATR 9 cut(s) 285, 369, 414, 420, 471, 528, 563, 664, 676
FatI CATG 4 cut(s) 283, 412, 662, 674
FblI GTMKAC 2 cut(s) 153, 419
FokI GGATG 3 cut(s) 48, 476, 578
FspBI CTAG 3 cut(s) 231, 359, 384
GlaI GCGC 2 cut(s) 381, 534
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 1 cut(s) 375
HapII CCGG 4 cut(s) 167, 180, 201, 461
HhaI GCGC 2 cut(s) 382, 535
Hin1I GRCGYC 2 cut(s) 150, 533
Hin1II CATG 4 cut(s) 287, 416, 666, 678
Hin6I GCGC 2 cut(s) 380, 533
HinP1I GCGC 2 cut(s) 380, 533
HinfI GANTC 2 cut(s) 57, 391
HpaII CCGG 4 cut(s) 167, 180, 201, 461
HphI GGTGA 3 cut(s) 37, 418, 530
Hpy166II GTNNAC 4 cut(s) 154, 420, 487, 500
Hpy188I TCNGA 4 cut(s) 56, 96, 559, 606
Hpy188III TCNNGA 1 cut(s) 675
Hpy8I GTNNAC 4 cut(s) 154, 420, 487, 500
Hpy99I CGWCG 1 cut(s) 152
HpyCH4III ACNGT 3 cut(s) 336, 518, 653
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 4 cut(s) 211, 412, 427, 480
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 2 cut(s) 150, 533
Hsp92II CATG 4 cut(s) 287, 416, 666, 678
HspAI GCGC 2 cut(s) 380, 533
KasI GGCGCC 1 cut(s) 532
Kzo9I GATC 2 cut(s) 27, 310
LmnI GCTCC 2 cut(s) 172, 436
LweI GCATC 2 cut(s) 399, 454
MaeI CTAG 3 cut(s) 231, 359, 384
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 4 cut(s) 298, 637, 647, 653
MalI GATC 2 cut(s) 29, 312
MboI GATC 2 cut(s) 27, 310
MboII GAAGA 3 cut(s) 83, 454, 560
MluCI AATT 3 cut(s) 235, 328, 594
Mly113I GGCGCC 1 cut(s) 533
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 7 cut(s) 67, 95, 163, 427, 538, 617, 660
Mph1103I ATGCAT 1 cut(s) 414
MseI TTAA 2 cut(s) 540, 630
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 4 cut(s) 167, 180, 201, 461
MspR9I CCNGG 2 cut(s) 48, 180
MvaI CCWGG 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 533
NciI CCSGG 1 cut(s) 180
NdeII GATC 2 cut(s) 27, 310
NlaIII CATG 4 cut(s) 287, 416, 666, 678
NlaIV GGNNCC 4 cut(s) 53, 122, 507, 534
NmuCI GTSAC 1 cut(s) 637
NsiI ATGCAT 1 cut(s) 414
PagI TCATGA 1 cut(s) 674
PfeI GAWTC 2 cut(s) 57, 391
PflFI GACNNNGTC 1 cut(s) 504
PluTI GGCGCC 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 4 cut(s) 53, 122, 507, 534
PspPI GGNCC 1 cut(s) 506
PsyI GACNNNGTC 1 cut(s) 504
RsaI GTAC 2 cut(s) 245, 459
RsaNI GTAC 2 cut(s) 244, 458
SaqAI TTAA 2 cut(s) 540, 630
Sau3AI GATC 2 cut(s) 27, 310
Sau96I GGNCC 1 cut(s) 506
ScrFI CCNGG 2 cut(s) 48, 180
SetI ASST 8 cut(s) 122, 129, 153, 177, 210, 259, 441, 486
SfaNI GCATC 2 cut(s) 399, 454
SfoI GGCGCC 1 cut(s) 534
SinI GGWCC 1 cut(s) 506
Sse9I AATT 3 cut(s) 235, 328, 594
SsiI CCGC 1 cut(s) 14
SspDI GGCGCC 1 cut(s) 532
SspMI CTAG 3 cut(s) 231, 359, 384
StyD4I CCNGG 2 cut(s) 46, 178
TaaI ACNGT 3 cut(s) 336, 518, 653
TaiI ACGT 1 cut(s) 153
TasI AATT 3 cut(s) 235, 328, 594
TfiI GAWTC 2 cut(s) 57, 391
Tru1I TTAA 2 cut(s) 540, 630
Tru9I TTAA 2 cut(s) 540, 630
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 1 cut(s) 637
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 2 cut(s) 582, 663
TspRI CASTG 1 cut(s) 591
Tth111I GACNNNGTC 1 cut(s) 504
VpaK11BI GGWCC 1 cut(s) 506
XmiI GTMKAC 2 cut(s) 153, 419
XspI CTAG 3 cut(s) 231, 359, 384
ZraI GACGTC 1 cut(s) 151
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.