MD10G1066200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005667
Physical Location & Seq
Forward (+)
9057445 .. 9058029
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1066200.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGATTTGGGAAAAATTTCAAGGGCAAACTCCATGGACGAAGAATTCGAATTCTTGGAAATTGAAGACGGAGAAGAACTCTTGGGTCTCACACACTGGGAATTCATCGACGCCTCCGACGCCGACTCAGAGGAAAACCACCACCACCACTGCTCCGACTCCGAACAAACGGATAAAGACGAAGAAGCAAATCGGTTCGATAGGCTTATCATCGGGCCCGCTTCATTTTCCCCTTCGATCCCTCCGCCGGTGGCCGATCCGATCCCCGCCCAACGTCGTCGCCGCCCCCATCTCGTCCGATTTGTGGATGTGGGTCTCAATCTCAAACTCCGTAGCGATTGGGACGACGGCAGCGATGAGGATGAGGGTCATGGTGAGGAGGAGGAGGAGGAGGAGGATGGGTATGGATTAGACGACGAGCTGGTGCCGTGGTCGGTGAGCGACAAGTTCGGGCGGCAGAGGATGAGGAAATTGGGGAAGAGGATGATTCCGAAGACGAACAATTCGAAGCGGTCTCCGTACATGGTTGCGAGGCCTGGCTGCGTTCGCGGGAAGCATGGCCTGGGATTGAAGCATATTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

22.19

Weight (kDa)

4.84

Isoelectric Point (pI)

64.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 424
AccII CGCG 1 cut(s) 549
AciI CCGC 7 cut(s) 219, 245, 267, 283, 454, 511, 549
AclWI GGATC 3 cut(s) 232, 251, 256
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 4 cut(s) 15, 44, 50, 101
AcyI GRCGYC 2 cut(s) 111, 120
AfaI GTAC 1 cut(s) 521
AfiI CCNNNNNNNGG 2 cut(s) 247, 304
AgsI TTSAA 3 cut(s) 21, 65, 571
AjnI CCWGG 2 cut(s) 535, 561
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Alw26I GTCTC 3 cut(s) 92, 320, 519
AlwI GGATC 3 cut(s) 232, 251, 256
AoxI GGCC 4 cut(s) 215, 252, 533, 559
ApaI GGGCCC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 351, 540
ApoI RAATTY 4 cut(s) 15, 44, 50, 101
ArsI GACNNNNNNTTYG 4 cut(s) 29, 61, 487, 519
Asp700I GAANNNNTTC 1 cut(s) 16
AspS9I GGNCC 2 cut(s) 215, 216
AsuHPI GGTGA 2 cut(s) 386, 448
AsuII TTCGAA 2 cut(s) 48, 506
BaeGI GKGCMC 1 cut(s) 219
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 219
BarI GAAGNNNNNNTAC 1 cut(s) 563
BbsI GAAGAC 2 cut(s) 72, 500
BbvI GCAGC 2 cut(s) 363, 527
BccI CCATC 2 cut(s) 297, 392
BceAI ACGGC 2 cut(s) 364, 412
BciT130I CCWGG 2 cut(s) 537, 563
BcoDI GTCTC 3 cut(s) 92, 320, 519
BisI GCNGC 4 cut(s) 283, 352, 455, 541
BlsI GCNGC 4 cut(s) 284, 353, 456, 542
Bme1390I CCNGG 2 cut(s) 537, 563
BmgT120I GGNCC 2 cut(s) 215, 216
BmiI GGNNCC 2 cut(s) 217, 426
BmrFI CCNGG 2 cut(s) 537, 563
BmrI ACTGGG 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 2 cut(s) 72, 500
BplI GAGNNNNNCTC 2 cut(s) 63, 95
Bpu14I TTCGAA 2 cut(s) 48, 506
BsaHI GRCGYC 2 cut(s) 111, 120
BsaI GGTCTC 3 cut(s) 92, 320, 519
BsaJI CCNNGG 3 cut(s) 33, 428, 562
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 386, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 304
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 2 cut(s) 537, 563
BseDI CCNNGG 3 cut(s) 33, 428, 562
BseGI GGATG 5 cut(s) 313, 367, 403, 468, 489
BseLI CCNNNNNNNGG 2 cut(s) 247, 304
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 6 cut(s) 392, 395, 398, 401, 404, 407
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 2 cut(s) 363, 527
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 4 cut(s) 217, 254, 535, 561
BshNI GGYRCC 1 cut(s) 424
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 2 cut(s) 247, 304
BsmAI GTCTC 3 cut(s) 92, 320, 519
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 4 cut(s) 217, 254, 535, 561
Bso31I GGTCTC 3 cut(s) 92, 320, 519
Bsp119I TTCGAA 2 cut(s) 48, 506
Bsp120I GGGCCC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 3 cut(s) 237, 256, 261
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 7 cut(s) 219, 245, 267, 283, 454, 511, 549
BspANI GGCC 4 cut(s) 217, 254, 535, 561
BspCNI CTCAG 1 cut(s) 140
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 2 cut(s) 217, 426
BspPI GGATC 3 cut(s) 232, 251, 256
BspT104I TTCGAA 2 cut(s) 48, 506
BspT107I GGYRCC 1 cut(s) 424
BspTNI GGTCTC 3 cut(s) 92, 320, 519
BsrFI RCCGGY 1 cut(s) 247
BsrI ACTGG 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 3 cut(s) 33, 428, 562
BssMI GATC 3 cut(s) 237, 256, 261
BssNI GRCGYC 2 cut(s) 111, 120
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 537, 563
Bst6I CTCTTC 1 cut(s) 473
BstACI GRCGYC 2 cut(s) 111, 120
BstBI TTCGAA 2 cut(s) 48, 506
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 127
BstDSI CCRYGG 2 cut(s) 33, 428
BstF5I GGATG 5 cut(s) 313, 367, 403, 468, 489
BstFNI CGCG 1 cut(s) 549
BstKTI GATC 3 cut(s) 240, 259, 264
BstMAI GTCTC 3 cut(s) 92, 320, 519
BstMBI GATC 3 cut(s) 237, 256, 261
BstMWI GCNNNNNNNGC 2 cut(s) 119, 546
BstNI CCWGG 2 cut(s) 537, 563
BstSCI CCNGG 2 cut(s) 535, 561
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 2 cut(s) 363, 527
BstV2I GAAGAC 2 cut(s) 72, 500
BsuRI GGCC 4 cut(s) 217, 254, 535, 561
BtgI CCRYGG 2 cut(s) 33, 428
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 5 cut(s) 313, 367, 403, 468, 489
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 2 cut(s) 94, 148
Cac8I GCNNGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 2 cut(s) 215, 216
CseI GACGC 2 cut(s) 119, 128
Csp6I GTAC 1 cut(s) 520
CviAII CATG 4 cut(s) 34, 371, 523, 557
CviJI RGCY 7 cut(s) 205, 217, 254, 421, 535, 540, 561
CviKI_1 RGCY 7 cut(s) 205, 217, 254, 421, 535, 540, 561
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 1 cut(s) 127
DpnI GATC 3 cut(s) 239, 258, 263
DpnII GATC 3 cut(s) 237, 256, 261
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 473
EciI GGCGGA 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 33
Eco147I AGGCCT 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 3 cut(s) 92, 320, 519
EcoRI GAATTC 3 cut(s) 44, 50, 101
EcoRII CCWGG 2 cut(s) 535, 561
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 4 cut(s) 37, 374, 526, 560
FaiI YATR 6 cut(s) 35, 372, 405, 524, 558, 576
FaqI GGGAC 1 cut(s) 356
FatI CATG 4 cut(s) 33, 370, 522, 556
FauI CCCGC 3 cut(s) 226, 274, 542
Fnu4HI GCNGC 4 cut(s) 283, 352, 455, 541
FokI GGATG 5 cut(s) 320, 374, 410, 475, 496
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 4 cut(s) 283, 352, 455, 541
GluI GCNGC 4 cut(s) 283, 352, 455, 541
HaeIII GGCC 4 cut(s) 217, 254, 535, 561
HapII CCGG 1 cut(s) 248
HgaI GACGC 2 cut(s) 119, 128
Hin1I GRCGYC 2 cut(s) 111, 120
Hin1II CATG 4 cut(s) 37, 374, 526, 560
HinfI GANTC 3 cut(s) 125, 158, 487
HpaII CCGG 1 cut(s) 248
HphI GGTGA 2 cut(s) 386, 448
Hpy188I TCNGA 7 cut(s) 118, 130, 157, 163, 261, 299, 492
Hpy99I CGWCG 6 cut(s) 113, 122, 279, 282, 350, 419
HpyAV CCTTC 1 cut(s) 243
HpyCH4IV ACGT 1 cut(s) 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 546
HpyF3I CTNAG 1 cut(s) 127
HpySE526I ACGT 1 cut(s) 274
Hsp92I GRCGYC 2 cut(s) 111, 120
Hsp92II CATG 4 cut(s) 37, 374, 526, 560
Kzo9I GATC 3 cut(s) 237, 256, 261
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 7 cut(s) 82, 261, 407, 522, 548, 549, 575
Lsp1109I GCAGC 2 cut(s) 363, 527
MaeII ACGT 1 cut(s) 274
MalI GATC 3 cut(s) 239, 258, 263
MboI GATC 3 cut(s) 237, 256, 261
MboII GAAGA 6 cut(s) 53, 77, 86, 194, 490, 505
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 7 cut(s) 15, 44, 50, 60, 101, 470, 502
MlyI GAGTC 2 cut(s) 119, 152
MmeI TCCRAC 2 cut(s) 141, 180
MroXI GAANNNNTTC 1 cut(s) 16
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 2 cut(s) 537, 563
MvaI CCWGG 2 cut(s) 537, 563
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 2 cut(s) 119, 546
NcoI CCATGG 1 cut(s) 33
NdeII GATC 3 cut(s) 237, 256, 261
NlaIII CATG 4 cut(s) 37, 374, 526, 560
NlaIV GGNNCC 2 cut(s) 217, 426
NspV TTCGAA 2 cut(s) 48, 506
PceI AGGCCT 1 cut(s) 535
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 114, 351, 503
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 487
PkrI GCNGC 4 cut(s) 284, 353, 456, 542
PleI GAGTC 2 cut(s) 119, 152
PpsI GAGTC 2 cut(s) 119, 152
Psp6I CCWGG 2 cut(s) 535, 561
PspGI CCWGG 2 cut(s) 535, 561
PspN4I GGNNCC 2 cut(s) 217, 426
PspOMI GGGCCC 1 cut(s) 215
PspPI GGNCC 2 cut(s) 215, 216
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
SatI GCNGC 4 cut(s) 283, 352, 455, 541
Sau3AI GATC 3 cut(s) 237, 256, 261
Sau96I GGNCC 2 cut(s) 215, 216
SchI GAGTC 2 cut(s) 119, 152
ScrFI CCNGG 2 cut(s) 537, 563
SduI GDGCHC 1 cut(s) 219
SetI ASST 2 cut(s) 277, 423
SfuI TTCGAA 2 cut(s) 48, 506
SgrAI CRCCGGYG 1 cut(s) 247
Sse9I AATT 7 cut(s) 15, 44, 50, 60, 101, 470, 502
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 7 cut(s) 219, 245, 267, 283, 454, 511, 549
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 2 cut(s) 535, 561
StyI CCWWGG 1 cut(s) 33
TaiI ACGT 1 cut(s) 277
TaqI TCGA 5 cut(s) 48, 108, 198, 236, 506
TasI AATT 7 cut(s) 15, 44, 50, 60, 101, 470, 502
TauI GCSGC 2 cut(s) 285, 457
TfiI GAWTC 1 cut(s) 487
TscAI CASTG 2 cut(s) 101, 155
TseI GCWGC 2 cut(s) 351, 540
TspDTI ATGAA 2 cut(s) 94, 213
TspGWI ACGGA 4 cut(s) 84, 185, 320, 507
TspRI CASTG 2 cut(s) 101, 155
XapI RAATTY 4 cut(s) 15, 44, 50, 101
XmnI GAANNNNTTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.