Rroxscaffold_1G00048060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005667
Physical Location & Seq
Forward (+)
68078861 .. 68079550
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048060.1

Sequence Viewer

Length: 450 bp
ATGGCAAGTTCCGTCGGCGGCTTCGAAGATTTCAAATTCTTGCAATTCGAAGACTCCAATCAAGACTCTCTCCGGGAATTGATCGACCTCTCCAACGCCAACTCCGATGACAACCTCCCCATCTCAACTCCAACGCCGAACCACCACAAAACTTCGATTGGGCCCAACACTTCATTCGACTCCCCAATCGCTTCGGCCATCATCGACCTGATCCGAGTCCACACCCGCCGTCATCGGACCCCTCTCGTTCGCCTTCTCGATCTACCTTTTACGAGCCCTCTCAATTCAATGACCGCGGTGGCCATGATGATGATGACGTCGGCGCTCATGGATTGTGATGTTCTGGATGACTATAAGCCAAGGACCAAAATATCGAGTTTCGAGGAAATTTCGATGGTCCCGAAAACGGAAATTTCAATGGAAATATCGAGGATATATCGATTTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.84

Weight (kDa)

5.27

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 320
AccII CGCG 1 cut(s) 296
AciI CCGC 4 cut(s) 18, 226, 294, 296
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 2 cut(s) 195, 300
AcsI RAATTY 3 cut(s) 35, 387, 411
AcyI GRCGYC 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 3 cut(s) 34, 288, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AlwI GGATC 1 cut(s) 205
AoxI GGCC 3 cut(s) 161, 195, 300
ApaI GGGCCC 1 cut(s) 165
ApoI RAATTY 3 cut(s) 35, 387, 411
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 5 cut(s) 161, 162, 237, 363, 397
AsuC2I CCSGG 1 cut(s) 74
AsuII TTCGAA 2 cut(s) 24, 48
AvaII GGWCC 3 cut(s) 237, 363, 397
BaeGI GKGCMC 1 cut(s) 165
BalI TGGCCA 1 cut(s) 302
BanII GRGCYC 2 cut(s) 165, 278
BbsI GAAGAC 1 cut(s) 57
BccI CCATC 3 cut(s) 128, 206, 388
BceAI ACGGC 1 cut(s) 213
BcnI CCSGG 1 cut(s) 74
BfoI RGCGCY 1 cut(s) 326
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 3 cut(s) 237, 363, 397
BmgT120I GGNCC 5 cut(s) 161, 162, 237, 363, 397
BmiI GGNNCC 3 cut(s) 163, 239, 399
BmrFI CCNGG 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 57
Bpu14I TTCGAA 2 cut(s) 24, 48
BpuMI CCSGG 1 cut(s) 74
Bsa29I ATCGAT 1 cut(s) 439
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 294, 359
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 294, 359
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 406
BseSI GKGCMC 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 3 cut(s) 163, 197, 302
BshVI ATCGAT 1 cut(s) 439
BsiSI CCGG 1 cut(s) 73
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 3 cut(s) 163, 197, 302
Bsp119I TTCGAA 2 cut(s) 24, 48
Bsp120I GGGCCC 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 165, 278
Bsp143I GATC 3 cut(s) 81, 210, 259
BspACI CCGC 4 cut(s) 18, 226, 294, 296
BspANI GGCC 3 cut(s) 163, 197, 302
BspDI ATCGAT 1 cut(s) 439
BspFNI CGCG 1 cut(s) 296
BspLI GGNNCC 3 cut(s) 163, 239, 399
BspPI GGATC 1 cut(s) 205
BspT104I TTCGAA 2 cut(s) 24, 48
BssECI CCNNGG 2 cut(s) 294, 359
BssMI GATC 3 cut(s) 81, 210, 259
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 359
BstACI GRCGYC 1 cut(s) 317
BstBI TTCGAA 2 cut(s) 24, 48
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 3 cut(s) 84, 213, 262
BstMBI GATC 3 cut(s) 81, 210, 259
BstSCI CCNGG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 165
BstUI CGCG 1 cut(s) 296
BstV2I GAAGAC 1 cut(s) 57
Bsu15I ATCGAT 1 cut(s) 439
BsuRI GGCC 3 cut(s) 163, 197, 302
BsuTUI ATCGAT 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 352
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 5 cut(s) 161, 162, 237, 363, 397
Cfr42I CCGCGG 1 cut(s) 297
ClaI ATCGAT 1 cut(s) 439
CviAII CATG 2 cut(s) 304, 328
CviJI RGCY 6 cut(s) 21, 163, 197, 276, 302, 358
CviKI_1 RGCY 6 cut(s) 21, 163, 197, 276, 302, 358
DpnI GATC 3 cut(s) 83, 212, 261
DpnII GATC 3 cut(s) 81, 210, 259
EaeI YGGCCR 2 cut(s) 195, 300
Eco130I CCWWGG 1 cut(s) 359
Eco24I GRGCYC 2 cut(s) 165, 278
Eco47I GGWCC 3 cut(s) 237, 363, 397
EcoT14I CCWWGG 1 cut(s) 359
EcoT38I GRGCYC 2 cut(s) 165, 278
ErhI CCWWGG 1 cut(s) 359
FaeI CATG 2 cut(s) 307, 331
FaiI YATR 4 cut(s) 305, 329, 354, 436
FaqI GGGAC 1 cut(s) 383
FatI CATG 2 cut(s) 303, 327
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 2 cut(s) 165, 278
Fsp4HI GCNGC 1 cut(s) 19
GlaI GCGC 1 cut(s) 324
GluI GCNGC 1 cut(s) 19
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 3 cut(s) 163, 197, 302
HapII CCGG 1 cut(s) 73
HhaI GCGC 1 cut(s) 325
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 2 cut(s) 307, 331
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HinfI GANTC 4 cut(s) 53, 65, 179, 216
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 3 cut(s) 106, 215, 237
Hpy188III TCNNGA 4 cut(s) 62, 257, 344, 400
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 2 cut(s) 17, 322
HpyAV CCTTC 1 cut(s) 263
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 43
HpySE526I ACGT 1 cut(s) 317
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 2 cut(s) 307, 331
HspAI GCGC 1 cut(s) 323
KspI CCGCGG 1 cut(s) 297
Kzo9I GATC 3 cut(s) 81, 210, 259
LpnPI CCDG 3 cut(s) 86, 221, 329
MaeII ACGT 1 cut(s) 317
MalI GATC 3 cut(s) 83, 212, 261
MboI GATC 3 cut(s) 81, 210, 259
MboII GAAGA 2 cut(s) 38, 62
MhlI GDGCHC 2 cut(s) 165, 278
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 6 cut(s) 35, 44, 77, 283, 387, 411
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 4 cut(s) 47, 59, 173, 225
MmeI TCCRAC 2 cut(s) 117, 155
MnlI CCTC 6 cut(s) 98, 125, 252, 288, 376, 423
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MslI CAYNNNNRTG 1 cut(s) 308
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 1 cut(s) 74
MvnI CGCG 1 cut(s) 296
NciI CCSGG 1 cut(s) 74
NdeII GATC 3 cut(s) 81, 210, 259
NlaIII CATG 2 cut(s) 307, 331
NlaIV GGNNCC 3 cut(s) 163, 239, 399
NspV TTCGAA 2 cut(s) 24, 48
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 102, 398
PfoI TCCNGGA 1 cut(s) 72
PkrI GCNGC 1 cut(s) 20
PleI GAGTC 4 cut(s) 47, 59, 173, 224
PpsI GAGTC 4 cut(s) 47, 59, 173, 224
PspN4I GGNNCC 3 cut(s) 163, 239, 399
PspOMI GGGCCC 1 cut(s) 161
PspPI GGNCC 5 cut(s) 161, 162, 237, 363, 397
RseI CAYNNNNRTG 1 cut(s) 308
SacII CCGCGG 1 cut(s) 297
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 3 cut(s) 81, 210, 259
Sau96I GGNCC 5 cut(s) 161, 162, 237, 363, 397
SchI GAGTC 4 cut(s) 47, 59, 173, 225
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 2 cut(s) 165, 278
SetI ASST 5 cut(s) 90, 117, 210, 268, 320
Sfr303I CCGCGG 1 cut(s) 297
SfuI TTCGAA 2 cut(s) 24, 48
SgrBI CCGCGG 1 cut(s) 297
SinI GGWCC 3 cut(s) 237, 363, 397
SmiMI CAYNNNNRTG 1 cut(s) 308
Sse9I AATT 6 cut(s) 35, 44, 77, 283, 387, 411
SsiI CCGC 4 cut(s) 18, 226, 294, 296
StyD4I CCNGG 1 cut(s) 72
StyI CCWWGG 1 cut(s) 359
TaiI ACGT 1 cut(s) 320
TasI AATT 6 cut(s) 35, 44, 77, 283, 387, 411
TauI GCSGC 1 cut(s) 21
TspDTI ATGAA 1 cut(s) 162
TspGWI ACGGA 1 cut(s) 422
VpaK11BI GGWCC 3 cut(s) 237, 363, 397
XapI RAATTY 3 cut(s) 35, 387, 411
ZraI GACGTC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.