Rh4DG245500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005667
Physical Location & Seq
Reverse (-)
45757353 .. 45757580
228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG245500.1

Sequence Viewer

Length: 228 bp
ATGGCAAGTTCCGTCAGTAGCTTCGAAGATTTTGAATTCTTGCAATTCGAAGACTCCGATCAAGACTCTCTCTGGGAATTCATCGACCTCTCCGACGTTGACTCCGACGACAACCTCCCAACCTCAACTCTGATGCCAAACCATCACAAAACTCCGAGCTCAGTGGGCCCAACACTTCATTCGACTCCCCATGGCCATCGCCGACCCCTTTGCCCATCGCTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

75

Amino Acids

8.4

Weight (kDa)

4.38

Isoelectric Point (pI)

65.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 2 cut(s) 35, 77
AgsI TTSAA 1 cut(s) 35
AjuI GAANNNNNNNTTGG 2 cut(s) 163, 195
AluBI AGCT 2 cut(s) 21, 159
AluI AGCT 2 cut(s) 21, 159
Alw21I GWGCWC 1 cut(s) 161
AoxI GGCC 2 cut(s) 166, 193
ApaI GGGCCC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 35, 77
AspS9I GGNCC 2 cut(s) 166, 167
AsuII TTCGAA 2 cut(s) 24, 48
BaeGI GKGCMC 1 cut(s) 170
BalI TGGCCA 1 cut(s) 195
BanII GRGCYC 2 cut(s) 161, 170
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 161
BccI CCATC 3 cut(s) 150, 204, 223
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BmgT120I GGNCC 2 cut(s) 166, 167
BmiI GGNNCC 1 cut(s) 168
BmsI GCATC 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 57
Bpu14I TTCGAA 2 cut(s) 24, 48
BsaJI CCNNGG 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
BseDI CCNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 170
BshFI GGCC 2 cut(s) 168, 195
BsiHKAI GWGCWC 1 cut(s) 161
BsnI GGCC 2 cut(s) 168, 195
Bsp119I TTCGAA 2 cut(s) 24, 48
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 2 cut(s) 161, 170
Bsp143I GATC 2 cut(s) 58, 222
Bsp19I CCATGG 1 cut(s) 190
BspANI GGCC 2 cut(s) 168, 195
BspCNI CTCAG 1 cut(s) 173
BspLI GGNNCC 1 cut(s) 168
BspT104I TTCGAA 2 cut(s) 24, 48
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 2 cut(s) 58, 222
BssT1I CCWWGG 1 cut(s) 190
BstBI TTCGAA 2 cut(s) 24, 48
BstDEI CTNAG 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 190
BstKTI GATC 2 cut(s) 61, 225
BstMBI GATC 2 cut(s) 58, 222
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 170
BstV2I GAAGAC 1 cut(s) 57
BsuRI GGCC 2 cut(s) 168, 195
BtgI CCRYGG 1 cut(s) 190
BtgZI GCGATG 2 cut(s) 182, 201
BtsIMutI CAGTG 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 166, 167
CviAII CATG 1 cut(s) 191
CviJI RGCY 4 cut(s) 21, 159, 168, 195
CviKI_1 RGCY 4 cut(s) 21, 159, 168, 195
DdeI CTNAG 1 cut(s) 160
DpnI GATC 2 cut(s) 60, 224
DpnII GATC 2 cut(s) 58, 222
EaeI YGGCCR 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 190
Eco24I GRGCYC 2 cut(s) 161, 170
Eco53kI GAGCTC 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 159
EcoRI GAATTC 2 cut(s) 35, 77
EcoT14I CCWWGG 1 cut(s) 190
EcoT38I GRGCYC 2 cut(s) 161, 170
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 1 cut(s) 194
FaiI YATR 1 cut(s) 192
FatI CATG 1 cut(s) 190
FriOI GRGCYC 2 cut(s) 161, 170
HaeIII GGCC 2 cut(s) 168, 195
Hin1II CATG 1 cut(s) 194
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 4 cut(s) 53, 65, 101, 184
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 6 cut(s) 58, 94, 106, 132, 156, 227
Hpy188III TCNNGA 1 cut(s) 62
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 98, 110
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 1 cut(s) 160
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 1 cut(s) 194
Kzo9I GATC 2 cut(s) 58, 222
LpnPI CCDG 1 cut(s) 58
LweI GCATC 1 cut(s) 123
MaeII ACGT 1 cut(s) 96
MalI GATC 2 cut(s) 60, 224
MboI GATC 2 cut(s) 58, 222
MboII GAAGA 2 cut(s) 38, 62
MhlI GDGCHC 2 cut(s) 161, 170
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 3 cut(s) 35, 44, 77
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 4 cut(s) 47, 59, 95, 178
MmeI TCCRAC 2 cut(s) 117, 129
MnlI CCTC 3 cut(s) 98, 125, 133
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
Msp20I TGGCCA 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 165
NcoI CCATGG 1 cut(s) 190
NdeII GATC 2 cut(s) 58, 222
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 1 cut(s) 168
NspV TTCGAA 2 cut(s) 24, 48
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 90, 102
PleI GAGTC 4 cut(s) 47, 59, 95, 178
PpsI GAGTC 4 cut(s) 47, 59, 95, 178
Psp124BI GAGCTC 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 168
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 2 cut(s) 166, 167
SacI GAGCTC 1 cut(s) 161
Sau3AI GATC 2 cut(s) 58, 222
Sau96I GGNCC 2 cut(s) 166, 167
SchI GAGTC 4 cut(s) 47, 59, 95, 178
SduI GDGCHC 2 cut(s) 161, 170
SetI ASST 6 cut(s) 23, 90, 99, 117, 125, 161
SfaNI GCATC 1 cut(s) 123
SfuI TTCGAA 2 cut(s) 24, 48
SgeI CNNG 6 cut(s) 18, 52, 74, 85, 168, 203
Sse9I AATT 3 cut(s) 35, 44, 77
SstI GAGCTC 1 cut(s) 161
StyI CCWWGG 1 cut(s) 190
TaiI ACGT 1 cut(s) 99
TaqI TCGA 4 cut(s) 24, 48, 84, 182
TasI AATT 3 cut(s) 35, 44, 77
TscAI CASTG 1 cut(s) 168
TspDTI ATGAA 2 cut(s) 70, 167
TspRI CASTG 1 cut(s) 168
XapI RAATTY 2 cut(s) 35, 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.