Rroxscaffold_7G00201250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005667
Physical Location & Seq
Forward (+)
48846093 .. 48846640
548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201250.1

Sequence Viewer

Length: 450 bp
ATGGCATCTTCCGTCAGCAGCTTCGAAGATTTCGAATTCTTGCAATTCGAAGACTCCGACCAAGACTCCCTCTGGGAATTCATCGACCTGTCCGACTCCGACTCCGACGACAACCACCCCCACCCCCACCCCAACTCCGACTCCGAACCACCACAACCCTCCGAGCCCATTGGGCCCGTCACTTCATTGGACTCCCCAATCGCTTCGCCCGTCGCCGACCCGATCCGAGCCCACACCCGCCGTCGTCAGACCCGTCTCGTTCGCCTTCTCGATCTTCCCTTCCAGACCCCCTCAGTTTCGACGACCGCCGTGGCCATGATGACGATGACGTCGGCGGCGGCGACGAGGAGGACGACGACGACAGGTACGGCCTTGACGACGAGCTGGTGCCGTGGTCGGCCTGGCTGCGTTCGCGGCAAGCATGGCCTGGGCTTGAAGCACATTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.35

Weight (kDa)

5.38

Isoelectric Point (pI)

59.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 328
AatII GACGTC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 387
AccII CGCG 1 cut(s) 414
AciI CCGC 5 cut(s) 238, 306, 335, 338, 414
AclWI GGATC 1 cut(s) 217
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 2 cut(s) 35, 77
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 1 cut(s) 367
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 2 cut(s) 400, 426
AluBI AGCT 2 cut(s) 21, 384
AluI AGCT 2 cut(s) 21, 384
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 1 cut(s) 217
AoxI GGCC 5 cut(s) 173, 312, 369, 398, 424
ApaI GGGCCC 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 18, 405
ApoI RAATTY 2 cut(s) 35, 77
AspS9I GGNCC 2 cut(s) 173, 174
AsuII TTCGAA 3 cut(s) 24, 33, 48
BaeGI GKGCMC 1 cut(s) 177
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 387
BanII GRGCYC 3 cut(s) 168, 177, 232
BarI GAAGNNNNNNTAC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 30, 392
BceAI ACGGC 4 cut(s) 225, 293, 375, 384
BciT130I CCWGG 2 cut(s) 402, 428
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 448
BglI GCCNNNNNGGC 1 cut(s) 172
BisI GCNGC 5 cut(s) 19, 336, 339, 406, 415
BlsI GCNGC 5 cut(s) 20, 337, 340, 407, 416
Bme1390I CCNGG 2 cut(s) 402, 428
BmgT120I GGNCC 2 cut(s) 173, 174
BmiI GGNNCC 2 cut(s) 175, 389
BmrFI CCNGG 2 cut(s) 402, 428
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 57
Bpu14I TTCGAA 3 cut(s) 24, 33, 48
BsaHI GRCGYC 1 cut(s) 329
BsaJI CCNNGG 3 cut(s) 309, 391, 427
BsaXI ACNNNNNCTCC 8 cut(s) 50, 80, 86, 116, 119, 125, 149, 155
BseBI CCWGG 2 cut(s) 402, 428
BseDI CCNNGG 3 cut(s) 309, 391, 427
BseMII CTCAG 1 cut(s) 306
BseRI GAGGAG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 177
BseXI GCAGC 2 cut(s) 30, 392
Bsh1236I CGCG 1 cut(s) 414
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 5 cut(s) 175, 314, 371, 400, 426
BshNI GGYRCC 1 cut(s) 387
BsiEI CGRYCG 1 cut(s) 306
BsmAI GTCTC 1 cut(s) 260
BsmBI CGTCTC 1 cut(s) 260
BsnI GGCC 5 cut(s) 175, 314, 371, 400, 426
Bsp119I TTCGAA 3 cut(s) 24, 33, 48
Bsp120I GGGCCC 1 cut(s) 173
Bsp1286I GDGCHC 3 cut(s) 168, 177, 232
Bsp143I GATC 2 cut(s) 222, 271
BspACI CCGC 5 cut(s) 238, 306, 335, 338, 414
BspANI GGCC 5 cut(s) 175, 314, 371, 400, 426
BspCNI CTCAG 1 cut(s) 305
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 175, 389
BspPI GGATC 1 cut(s) 217
BspT104I TTCGAA 3 cut(s) 24, 33, 48
BspT107I GGYRCC 1 cut(s) 387
BssECI CCNNGG 3 cut(s) 309, 391, 427
BssMI GATC 2 cut(s) 222, 271
BssNI GRCGYC 1 cut(s) 329
Bst2UI CCWGG 2 cut(s) 402, 428
BstACI GRCGYC 1 cut(s) 329
BstBI TTCGAA 3 cut(s) 24, 33, 48
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 1 cut(s) 292
BstDSI CCRYGG 2 cut(s) 309, 391
BstFNI CGCG 1 cut(s) 414
BstKTI GATC 2 cut(s) 225, 274
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 2 cut(s) 222, 271
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 4 cut(s) 172, 411, 414, 423
BstNI CCWGG 2 cut(s) 402, 428
BstSCI CCNGG 2 cut(s) 400, 426
BstSLI GKGCMC 1 cut(s) 177
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 30, 392
BstV2I GAAGAC 1 cut(s) 57
BsuRI GGCC 5 cut(s) 175, 314, 371, 400, 426
BtgI CCRYGG 2 cut(s) 309, 391
Cac8I GCNNGC 1 cut(s) 419
Cfr13I GGNCC 2 cut(s) 173, 174
Csp6I GTAC 1 cut(s) 366
CviAII CATG 2 cut(s) 316, 422
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 1 cut(s) 292
DpnI GATC 2 cut(s) 224, 273
DpnII GATC 2 cut(s) 222, 271
DrdI GACNNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 312
Eco24I GRGCYC 3 cut(s) 168, 177, 232
EcoRI GAATTC 2 cut(s) 35, 77
EcoRII CCWGG 2 cut(s) 400, 426
EcoT38I GRGCYC 3 cut(s) 168, 177, 232
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 2 cut(s) 319, 425
FaiI YATR 2 cut(s) 317, 423
FatI CATG 2 cut(s) 315, 421
FauI CCCGC 1 cut(s) 245
Fnu4HI GCNGC 5 cut(s) 19, 336, 339, 406, 415
FriOI GRGCYC 3 cut(s) 168, 177, 232
Fsp4HI GCNGC 5 cut(s) 19, 336, 339, 406, 415
FspBI CTAG 1 cut(s) 448
GluI GCNGC 5 cut(s) 19, 336, 339, 406, 415
HaeIII GGCC 5 cut(s) 175, 314, 371, 400, 426
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 2 cut(s) 319, 425
HinfI GANTC 6 cut(s) 53, 65, 95, 101, 140, 191
Hpy188I TCNGA 9 cut(s) 58, 94, 100, 106, 139, 145, 163, 227, 249
Hpy188III TCNNGA 2 cut(s) 269, 283
Hpy99I CGWCG 9 cut(s) 110, 215, 246, 304, 334, 346, 358, 361, 382
HpyAV CCTTC 2 cut(s) 275, 289
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 411, 414, 423
HpyF3I CTNAG 1 cut(s) 292
HpySE526I ACGT 1 cut(s) 329
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 2 cut(s) 319, 425
Kzo9I GATC 2 cut(s) 222, 271
LpnPI CCDG 9 cut(s) 58, 101, 296, 348, 370, 387, 413, 414, 440
Lsp1109I GCAGC 2 cut(s) 30, 392
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 448
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 1 cut(s) 178
MalI GATC 2 cut(s) 224, 273
MboI GATC 2 cut(s) 222, 271
MboII GAAGA 3 cut(s) 38, 62, 266
MhlI GDGCHC 3 cut(s) 168, 177, 232
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 3 cut(s) 35, 44, 77
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 6 cut(s) 47, 59, 89, 95, 134, 185
MmeI TCCRAC 5 cut(s) 81, 117, 123, 129, 162
MnlI CCTC 5 cut(s) 80, 169, 301, 339, 342
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
Msp20I TGGCCA 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 402, 428
MvaI CCWGG 2 cut(s) 402, 428
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 172, 411, 414, 423
NdeII GATC 2 cut(s) 222, 271
NlaIII CATG 2 cut(s) 319, 425
NlaIV GGNNCC 2 cut(s) 175, 389
NmuCI GTSAC 1 cut(s) 178
NspV TTCGAA 3 cut(s) 24, 33, 48
PcsI WCGNNNNNNNCGW 8 cut(s) 30, 54, 90, 207, 250, 338, 350, 374
PkrI GCNGC 5 cut(s) 20, 337, 340, 407, 416
PleI GAGTC 6 cut(s) 47, 59, 89, 95, 134, 185
PpsI GAGTC 6 cut(s) 47, 59, 89, 95, 134, 185
Psp6I CCWGG 2 cut(s) 400, 426
PspGI CCWGG 2 cut(s) 400, 426
PspN4I GGNNCC 2 cut(s) 175, 389
PspOMI GGGCCC 1 cut(s) 173
PspPI GGNCC 2 cut(s) 173, 174
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SatI GCNGC 5 cut(s) 19, 336, 339, 406, 415
Sau3AI GATC 2 cut(s) 222, 271
Sau96I GGNCC 2 cut(s) 173, 174
SchI GAGTC 6 cut(s) 47, 59, 89, 95, 134, 185
ScrFI CCNGG 2 cut(s) 402, 428
SduI GDGCHC 3 cut(s) 168, 177, 232
SetI ASST 5 cut(s) 23, 90, 332, 367, 386
SfaNI GCATC 1 cut(s) 14
SfuI TTCGAA 3 cut(s) 24, 33, 48
Sse9I AATT 3 cut(s) 35, 44, 77
SsiI CCGC 5 cut(s) 238, 306, 335, 338, 414
SspMI CTAG 1 cut(s) 448
StyD4I CCNGG 2 cut(s) 400, 426
TaiI ACGT 1 cut(s) 332
TaqI TCGA 6 cut(s) 24, 33, 48, 84, 270, 299
TasI AATT 3 cut(s) 35, 44, 77
TauI GCSGC 3 cut(s) 338, 341, 417
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 2 cut(s) 18, 405
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 70, 174
XapI RAATTY 2 cut(s) 35, 77
XspI CTAG 1 cut(s) 448
ZraI GACGTC 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.