pycom05g04950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005667
Physical Location & Seq
Forward (+)
5850783 .. 5851373
591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04950.1

Sequence Viewer

Length: 591 bp
ATGGATTTGGGAAAACTTTCGAATGCAAACTCCATGGACGAACAATTCGAATTCCTGGAAATCGAAGACGGAGAAGAACTCTTGGGTCTCACACACTGGGAATTCATCGACGCCTCCGACGCCCACTCAGAGCAAAACCACCACCACCACCACCGCTCCGACTGCGAACAAACCGATAAAGACGAAGAAGCAAATCGGTTTGATAGGCTTATCATTGGGCCCGCTTCATTTTCCCCTTCGGTCCCTCCGCCGGTGGCCGATCCGATCCCCGCCCACCGTCATCGCCGCCCCCGTCTCGTCCGATTTGTGGATGTGGGTCTCAATCTCAAATTCCATAGCGATTGGGACGACAGCGGCGATGAGGATGAGGGCCATGGTGAGGAGGAGGAGGAGGAGGAGGATTATGGGTATGGATTAGACGACGAGCTGGTGCCGTTGTCGGTGAGCGACAAGTTGGGGCGGCAGAGGATGAGGAAATTGGGGAAGAGGATGTTACCGAAGATGAACAAATCGAAGCGGTCGCCGTACATGGTTGCGAGGCCTGGCTGCGTTCGCGGGAAGCATGGCCTGGGATTGAAGCATATTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.49

Weight (kDa)

5.25

Isoelectric Point (pI)

57.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 156
AccII CGCG 1 cut(s) 555
AciI CCGC 9 cut(s) 154, 222, 248, 270, 286, 354, 460, 517, 555
AclWI GGATC 2 cut(s) 254, 259
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 3 cut(s) 50, 101, 329
AcyI GRCGYC 2 cut(s) 111, 120
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 3 cut(s) 250, 307, 379
AgsI TTSAA 1 cut(s) 577
AjnI CCWGG 3 cut(s) 54, 541, 567
AluBI AGCT 1 cut(s) 427
AluI AGCT 1 cut(s) 427
Alw26I GTCTC 3 cut(s) 92, 299, 323
AlwI GGATC 2 cut(s) 254, 259
AoxI GGCC 5 cut(s) 218, 255, 370, 539, 565
ApaI GGGCCC 1 cut(s) 222
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 3 cut(s) 50, 101, 329
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
Asp700I GAANNNNTTC 1 cut(s) 16
AspS9I GGNCC 4 cut(s) 218, 219, 241, 370
AsuHPI GGTGA 2 cut(s) 389, 454
AsuII TTCGAA 2 cut(s) 20, 48
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 430
BanII GRGCYC 1 cut(s) 222
BarI GAAGNNNNNNTAC 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 533
BceAI ACGGC 2 cut(s) 418, 508
BciT130I CCWGG 3 cut(s) 56, 543, 569
BcoDI GTCTC 3 cut(s) 92, 299, 323
BisI GCNGC 4 cut(s) 286, 355, 461, 547
BlsI GCNGC 4 cut(s) 287, 356, 462, 548
Bme1390I CCNGG 3 cut(s) 56, 543, 569
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 218, 219, 241, 370
BmiI GGNNCC 3 cut(s) 220, 243, 432
BmrFI CCNGG 3 cut(s) 56, 543, 569
BmrI ACTGGG 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 63, 95
Bpu14I TTCGAA 2 cut(s) 20, 48
BsaHI GRCGYC 2 cut(s) 111, 120
BsaI GGTCTC 2 cut(s) 92, 323
BsaJI CCNNGG 3 cut(s) 33, 373, 568
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 307, 379
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 3 cut(s) 56, 543, 569
BseDI CCNNGG 3 cut(s) 33, 373, 568
BseGI GGATG 4 cut(s) 316, 370, 474, 495
BseLI CCNNNNNNNGG 3 cut(s) 250, 307, 379
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 6 cut(s) 395, 398, 401, 404, 407, 410
BseSI GKGCMC 1 cut(s) 222
BseXI GCAGC 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 555
Bsh1285I CGRYCG 1 cut(s) 521
BshFI GGCC 5 cut(s) 220, 257, 372, 541, 567
BshNI GGYRCC 1 cut(s) 430
BsiEI CGRYCG 1 cut(s) 521
BsiSI CCGG 1 cut(s) 251
BslFI GGGAC 2 cut(s) 227, 359
BslI CCNNNNNNNGG 3 cut(s) 250, 307, 379
BsmAI GTCTC 3 cut(s) 92, 299, 323
BsmBI CGTCTC 1 cut(s) 299
BsmFI GGGAC 2 cut(s) 227, 359
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 5 cut(s) 220, 257, 372, 541, 567
Bso31I GGTCTC 2 cut(s) 92, 323
Bsp119I TTCGAA 2 cut(s) 20, 48
Bsp120I GGGCCC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 2 cut(s) 259, 264
Bsp19I CCATGG 2 cut(s) 33, 373
BspACI CCGC 9 cut(s) 154, 222, 248, 270, 286, 354, 460, 517, 555
BspANI GGCC 5 cut(s) 220, 257, 372, 541, 567
BspCNI CTCAG 1 cut(s) 140
BspFNI CGCG 1 cut(s) 555
BspLI GGNNCC 3 cut(s) 220, 243, 432
BspPI GGATC 2 cut(s) 254, 259
BspT104I TTCGAA 2 cut(s) 20, 48
BspT107I GGYRCC 1 cut(s) 430
BspTNI GGTCTC 2 cut(s) 92, 323
BsrBI CCGCTC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 33, 373, 568
BssMI GATC 2 cut(s) 259, 264
BssNI GRCGYC 2 cut(s) 111, 120
BssT1I CCWWGG 2 cut(s) 33, 373
Bst2UI CCWGG 3 cut(s) 56, 543, 569
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 479
BstACI GRCGYC 2 cut(s) 111, 120
BstBI TTCGAA 2 cut(s) 20, 48
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 127
BstDSI CCRYGG 2 cut(s) 33, 373
BstF5I GGATG 4 cut(s) 316, 370, 474, 495
BstFNI CGCG 1 cut(s) 555
BstKTI GATC 2 cut(s) 262, 267
BstMAI GTCTC 3 cut(s) 92, 299, 323
BstMBI GATC 2 cut(s) 259, 264
BstMCI CGRYCG 1 cut(s) 521
BstMWI GCNNNNNNNGC 3 cut(s) 119, 162, 552
BstNI CCWGG 3 cut(s) 56, 543, 569
BstSCI CCNGG 3 cut(s) 54, 541, 567
BstSLI GKGCMC 1 cut(s) 222
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 72
BsuRI GGCC 5 cut(s) 220, 257, 372, 541, 567
BtgI CCRYGG 2 cut(s) 33, 373
BtgZI GCGATG 2 cut(s) 266, 372
BtsCI GGATG 4 cut(s) 316, 370, 474, 495
BtsIMutI CAGTG 1 cut(s) 94
Cac8I GCNNGC 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 4 cut(s) 218, 219, 241, 370
CseI GACGC 2 cut(s) 119, 128
Csp6I GTAC 1 cut(s) 526
CviAII CATG 4 cut(s) 34, 374, 529, 563
CviJI RGCY 8 cut(s) 208, 220, 257, 372, 427, 541, 546, 567
CviKI_1 RGCY 8 cut(s) 208, 220, 257, 372, 427, 541, 546, 567
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 1 cut(s) 127
DpnI GATC 2 cut(s) 261, 266
DpnII GATC 2 cut(s) 259, 264
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 479
EarI CTCTTC 1 cut(s) 479
EciI GGCGGA 1 cut(s) 237
Eco130I CCWWGG 2 cut(s) 33, 373
Eco147I AGGCCT 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 2 cut(s) 92, 323
Eco47I GGWCC 1 cut(s) 241
EcoRI GAATTC 2 cut(s) 50, 101
EcoRII CCWGG 3 cut(s) 54, 541, 567
EcoT14I CCWWGG 2 cut(s) 33, 373
EcoT38I GRGCYC 1 cut(s) 222
ErhI CCWWGG 2 cut(s) 33, 373
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 4 cut(s) 37, 377, 532, 566
FaiI YATR 8 cut(s) 35, 336, 375, 405, 411, 530, 564, 582
FaqI GGGAC 2 cut(s) 227, 359
FatI CATG 4 cut(s) 33, 373, 528, 562
FauI CCCGC 3 cut(s) 229, 277, 548
Fnu4HI GCNGC 4 cut(s) 286, 355, 461, 547
FokI GGATG 4 cut(s) 323, 377, 481, 502
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 4 cut(s) 286, 355, 461, 547
GluI GCNGC 4 cut(s) 286, 355, 461, 547
HaeIII GGCC 5 cut(s) 220, 257, 372, 541, 567
HapII CCGG 1 cut(s) 251
HgaI GACGC 2 cut(s) 119, 128
Hin1I GRCGYC 2 cut(s) 111, 120
Hin1II CATG 4 cut(s) 37, 377, 532, 566
HpaII CCGG 1 cut(s) 251
HphI GGTGA 2 cut(s) 389, 454
Hpy188I TCNGA 5 cut(s) 118, 130, 160, 264, 302
Hpy99I CGWCG 3 cut(s) 113, 122, 425
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 162, 552
HpyF3I CTNAG 1 cut(s) 127
Hsp92I GRCGYC 2 cut(s) 111, 120
Hsp92II CATG 4 cut(s) 37, 377, 532, 566
Kzo9I GATC 2 cut(s) 259, 264
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 9 cut(s) 41, 68, 82, 264, 413, 528, 554, 555, 581
Lsp1109I GCAGC 1 cut(s) 533
MaeIII GTNAC 1 cut(s) 492
MalI GATC 2 cut(s) 261, 266
MbiI CCGCTC 1 cut(s) 156
MboI GATC 2 cut(s) 259, 264
MboII GAAGA 5 cut(s) 77, 86, 197, 496, 511
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 5 cut(s) 44, 50, 101, 329, 476
MmeI TCCRAC 2 cut(s) 141, 183
MroXI GAANNNNTTC 1 cut(s) 16
MspA1I CMGCKG 1 cut(s) 354
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 3 cut(s) 56, 543, 569
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 3 cut(s) 56, 543, 569
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 119, 162, 552
NcoI CCATGG 2 cut(s) 33, 373
NdeII GATC 2 cut(s) 259, 264
NlaIII CATG 4 cut(s) 37, 377, 532, 566
NlaIV GGNNCC 3 cut(s) 220, 243, 432
NspV TTCGAA 2 cut(s) 20, 48
PceI AGGCCT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 114, 289, 354
PctI GAATGC 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 54
PkrI GCNGC 4 cut(s) 287, 356, 462, 548
Psp6I CCWGG 3 cut(s) 54, 541, 567
PspGI CCWGG 3 cut(s) 54, 541, 567
PspN4I GGNNCC 3 cut(s) 220, 243, 432
PspOMI GGGCCC 1 cut(s) 218
PspPI GGNCC 4 cut(s) 218, 219, 241, 370
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SatI GCNGC 4 cut(s) 286, 355, 461, 547
Sau3AI GATC 2 cut(s) 259, 264
Sau96I GGNCC 4 cut(s) 218, 219, 241, 370
ScrFI CCNGG 3 cut(s) 56, 543, 569
SduI GDGCHC 1 cut(s) 222
SetI ASST 1 cut(s) 429
SfuI TTCGAA 2 cut(s) 20, 48
SgrAI CRCCGGYG 1 cut(s) 250
SinI GGWCC 1 cut(s) 241
Sse9I AATT 5 cut(s) 44, 50, 101, 329, 476
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 9 cut(s) 154, 222, 248, 270, 286, 354, 460, 517, 555
StuI AGGCCT 1 cut(s) 541
StyD4I CCNGG 3 cut(s) 54, 541, 567
StyI CCWWGG 2 cut(s) 33, 373
TaaI ACNGT 1 cut(s) 278
TaqI TCGA 5 cut(s) 20, 48, 63, 108, 512
TaqII GACCGA 1 cut(s) 229
TasI AATT 5 cut(s) 44, 50, 101, 329, 476
TauI GCSGC 3 cut(s) 288, 357, 463
TscAI CASTG 1 cut(s) 101
TseI GCWGC 1 cut(s) 546
TspDTI ATGAA 3 cut(s) 94, 216, 518
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 3 cut(s) 50, 101, 329
XmnI GAANNNNTTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.