MD10G1082400.v1.1

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004525
Physical Location & Seq
Forward (+)
12120122 .. 12120517
396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1082400.v1.1.491

Sequence Viewer

Length: 396 bp
ATGTTTTATTTTGTAATTTCGAAGGCTATTGATGGTTATATGGAGATCATAAAGGTTGGTGATTCATCACGTGATATGGAAGATAATCAAGCTTCTTGTTTCAGATGCAACATCTGCCTTGAATTAGCTGATCAAGACCCAATCTTAACACCTTGTGGCCACCTCTACTGCTGGCCTTGTTTATACAGAAGGCTTTGTGCTCGCTCATCACTACTCAATAAGCCCCGCTGCCCAGTTTGTAGGGCTGTTGTAAAGAAAAACAAATTAATCCCTATTTTTGGAATAACGAAATTGTTACTTGATTCTGGTAAAAGAACTCCTTCTGTACCGCCCGGTTTTGAGATTCCAAATAGATCATCTCCATCTTCACCATCTTCGAATACTTCATCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

132

Amino Acids

14.58

Weight (kDa)

8.92

Isoelectric Point (pI)

71.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_5 PF14634 35 - 81 1.6e-08 zinc-RING finger domain
zf-C3HC4_3 PF13920 35 - 86 5.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 35 - 81 1.1e-06 Ring finger domain
zf-RING_UBOX PF13445 36 - 78 2.3e-09 RING-type zinc-finger
zf-C3HC4 PF00097 36 - 80 1.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 226, 329
AcoI YGGCCR 1 cut(s) 157
AcvI CACGTG 1 cut(s) 71
AdeI CACNNNGTG 1 cut(s) 155
AfaI GTAC 1 cut(s) 327
AfiI CCNNNNNNNGG 1 cut(s) 278
AgsI TTSAA 1 cut(s) 122
AluBI AGCT 2 cut(s) 92, 128
AluI AGCT 2 cut(s) 92, 128
Alw21I GWGCWC 1 cut(s) 202
AoxI GGCC 2 cut(s) 157, 173
ApeKI GCWGC 1 cut(s) 228
AseI ATTAAT 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 319
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 2 cut(s) 71, 360
AsuII TTCGAA 2 cut(s) 20, 377
BalI TGGCCA 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 215
BccI CCATC 3 cut(s) 26, 370, 379
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 333
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
Bme1390I CCNGG 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 227
Bpu14I TTCGAA 2 cut(s) 20, 377
BpuMI CCSGG 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 71
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 233
BseLI CCNNNNNNNGG 1 cut(s) 278
BseNI ACTGG 1 cut(s) 233
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 2 cut(s) 159, 175
BsiHKAI GWGCWC 1 cut(s) 202
BsiSI CCGG 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 278
BsnI GGCC 2 cut(s) 159, 175
Bsp119I TTCGAA 2 cut(s) 20, 377
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 3 cut(s) 45, 130, 353
BspACI CCGC 2 cut(s) 226, 329
BspANI GGCC 2 cut(s) 159, 175
BspT104I TTCGAA 2 cut(s) 20, 377
BsrI ACTGG 1 cut(s) 233
BssMI GATC 3 cut(s) 45, 130, 353
BstAPI GCANNNNNTGC 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 71
BstBI TTCGAA 2 cut(s) 20, 377
BstC8I GCNNGC 2 cut(s) 173, 202
BstKTI GATC 3 cut(s) 48, 133, 356
BstMBI GATC 3 cut(s) 45, 130, 353
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 215
BsuRI GGCC 2 cut(s) 159, 175
Cac8I GCNNGC 2 cut(s) 173, 202
Csp6I GTAC 1 cut(s) 326
CviJI RGCY 8 cut(s) 26, 92, 128, 159, 175, 193, 223, 245
CviKI_1 RGCY 8 cut(s) 26, 92, 128, 159, 175, 193, 223, 245
CviQI GTAC 1 cut(s) 326
DpnI GATC 3 cut(s) 47, 132, 355
DpnII GATC 3 cut(s) 45, 130, 353
DraIII CACNNNGTG 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 71
FaiI YATR 5 cut(s) 39, 41, 50, 77, 184
FalI AAGNNNNNCTT 2 cut(s) 304, 336
FauI CCCGC 1 cut(s) 233
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 229
GluI GCNGC 1 cut(s) 229
HaeIII GGCC 2 cut(s) 159, 175
HapII CCGG 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 3 cut(s) 62, 302, 343
HpaII CCGG 1 cut(s) 333
HphI GGTGA 2 cut(s) 71, 360
Hpy188I TCNGA 1 cut(s) 104
Hpy188III TCNNGA 1 cut(s) 134
HpyAV CCTTC 3 cut(s) 16, 183, 330
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 3 cut(s) 45, 130, 353
LpnPI CCDG 4 cut(s) 157, 246, 291, 346
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 1 cut(s) 95
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 294
MalI GATC 3 cut(s) 47, 132, 355
MboI GATC 3 cut(s) 45, 130, 353
MboII GAAGA 3 cut(s) 92, 357, 366
MhlI GDGCHC 1 cut(s) 202
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 4 cut(s) 15, 122, 263, 290
MluNI TGGCCA 1 cut(s) 159
MnlI CCTC 1 cut(s) 173
Mox20I TGGCCA 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 319
MscI TGGCCA 1 cut(s) 159
MseI TTAA 2 cut(s) 146, 266
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 333
NdeII GATC 3 cut(s) 45, 130, 353
NspV TTCGAA 2 cut(s) 20, 377
PdmI GAANNNNTTC 1 cut(s) 319
PfeI GAWTC 3 cut(s) 62, 302, 343
PkrI GCNGC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 266
PspCI CACGTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
SaqAI TTAA 2 cut(s) 146, 266
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 3 cut(s) 45, 130, 353
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 202
SetI ASST 6 cut(s) 57, 73, 94, 130, 154, 165
SfaNI GCATC 1 cut(s) 95
SfuI TTCGAA 2 cut(s) 20, 377
Sse9I AATT 4 cut(s) 15, 122, 263, 290
SsiI CCGC 2 cut(s) 226, 329
StyD4I CCNGG 1 cut(s) 331
TaiI ACGT 1 cut(s) 73
TaqI TCGA 2 cut(s) 20, 377
TasI AATT 4 cut(s) 15, 122, 263, 290
TfiI GAWTC 3 cut(s) 62, 302, 343
Tru1I TTAA 2 cut(s) 146, 266
Tru9I TTAA 2 cut(s) 146, 266
TseI GCWGC 1 cut(s) 228
TspDTI ATGAA 2 cut(s) 54, 375
VspI ATTAAT 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.