Rroxscaffold_177G00434410

ER-associated misfolded protein catabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0004525
Physical Location & Seq
Forward (+)
373860 .. 376156
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434410.1

Sequence Viewer

Length: 804 bp
ATGGATGGGGGTGATGGTGGTACTGGATTCACTTGCAACATTTGCTTTGAATCTGCTGATAAAGATCCAATTGTAACACCTTGTGGCCACCTTTACTGCAAGTCTTGTATATATACATGGCTTCATAACCCAAGACATGATTCCAAGAGGTGCCCTTGCCCTGTATGCAATGCTATTATAGAGGAGAGCAAACTGATTCCTCTGTATGGAATAGGAAAATCACAAGATTTAAATCAAAAGCCTATACCTGTAGCCAGTGTTGAGAGCCCAGAAAGGCAAGCTGGTCAAAAGTCTGCCACTACTTCAGCTCCATATAGGCCAACTCCTTCAGCTCCAGAGTGGCCGACTGGTCCAAGACATGCCACTTCTTCAGCTCCAGAGTGGCCAACTGATCCAACATCCATTCCTTCGGCTCCTGAATGGCCGACTGGTCCAAGACCTGCCACTCCTTCAGCTCCCATATGGACTGATCCAAGACCTGCCACTCTTTCAGCTTCAAACTGTTCATCCGATCAAACACTTGACACTCCTTCAACTGCCAATGTCATTCTGAACCAAGTTTGTAGGCTGTTAATACGTGTGGGACAGTACATGCTAGTGAGGATAATAGAAGTTGCCACAGATAAGCTCATTGAAGCTGTAGAAAGAAAAATCAATGAGTCGAGTGAGGCAGCAGAAGAGAGGAACGATGTTAATGAAGAAGGAAGTGGAAATAATACTGTATCAAGTGAGGAGGTTGAAGTGACGGATAGCGATGGAGAAGGAAGCCATCTGGGGCGCTGGGTTGTGCTCAACCCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

267

Amino Acids

28.8

Weight (kDa)

4.82

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 11 - 62 7.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 11 - 57 9.3e-07 Ring finger domain
zf-RING_UBOX PF13445 12 - 52 3.4e-09 RING-type zinc-finger
zf-C3HC4 PF00097 12 - 56 2.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 12 - 52 2.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 12 - 57 1.6e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 448, 487
AccB1I GGYRCC 1 cut(s) 150
AclWI GGATC 3 cut(s) 59, 386, 464
AcoI YGGCCR 4 cut(s) 85, 341, 383, 422
AcuI CTGAAG 4 cut(s) 288, 312, 354, 435
AdeI CACNNNGTG 1 cut(s) 83
AfaI GTAC 2 cut(s) 22, 590
AfiI CCNNNNNNNGG 1 cut(s) 206
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 5 cut(s) 50, 498, 534, 635, 740
AjuI GAANNNNNNNTTGG 2 cut(s) 124, 156
AluBI AGCT 8 cut(s) 281, 308, 332, 374, 455, 494, 628, 638
AluI AGCT 8 cut(s) 281, 308, 332, 374, 455, 494, 628, 638
Alw21I GWGCWC 1 cut(s) 792
AlwI GGATC 3 cut(s) 59, 386, 464
AoxI GGCC 5 cut(s) 85, 317, 341, 383, 422
ApeKI GCWGC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 780
AspS9I GGNCC 2 cut(s) 350, 431
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 2 cut(s) 350, 431
BaeGI GKGCMC 1 cut(s) 155
BalI TGGCCA 2 cut(s) 87, 385
BanI GGYRCC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 1 cut(s) 683
BccI CCATC 3 cut(s) 8, 749, 777
BfaI CTAG 1 cut(s) 596
BfmI CTRYAG 2 cut(s) 249, 639
BfoI RGCGCY 1 cut(s) 781
BfuAI ACCTGC 2 cut(s) 448, 487
BisI GCNGC 1 cut(s) 672
BlsI GCNGC 1 cut(s) 673
Bme18I GGWCC 2 cut(s) 350, 431
BmgT120I GGNCC 2 cut(s) 350, 431
BmiI GGNNCC 2 cut(s) 152, 414
BpmI CTGGAG 2 cut(s) 318, 360
BsaAI YACGTR 1 cut(s) 578
BsaBI GATNNNNATC 2 cut(s) 63, 231
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 4 cut(s) 28, 255, 352, 433
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 2 cut(s) 63, 231
BseGI GGATG 3 cut(s) 10, 398, 506
BseJI GATNNNNATC 2 cut(s) 63, 231
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 175
BseNI ACTGG 4 cut(s) 28, 255, 352, 433
BseRI GAGGAG 2 cut(s) 197, 746
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 1 cut(s) 683
BseYI CCCAGC 1 cut(s) 780
BshFI GGCC 5 cut(s) 87, 319, 343, 385, 424
BshNI GGYRCC 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 792
BslFI GGGAC 1 cut(s) 597
BslI CCNNNNNNNGG 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 597
BsnI GGCC 5 cut(s) 87, 319, 343, 385, 424
Bsp1286I GDGCHC 3 cut(s) 155, 269, 792
Bsp143I GATC 4 cut(s) 64, 391, 469, 511
BspANI GGCC 5 cut(s) 87, 319, 343, 385, 424
BspLI GGNNCC 2 cut(s) 152, 414
BspMI ACCTGC 2 cut(s) 448, 487
BspPI GGATC 3 cut(s) 59, 386, 464
BspT107I GGYRCC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 4 cut(s) 28, 255, 352, 433
BssMI GATC 4 cut(s) 64, 391, 469, 511
Bst4CI ACNGT 3 cut(s) 503, 588, 721
Bst6I CTCTTC 1 cut(s) 672
BstAPI GCANNNNNTGC 1 cut(s) 42
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 801
BstF5I GGATG 3 cut(s) 10, 398, 506
BstH2I RGCGCY 1 cut(s) 781
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 4 cut(s) 67, 394, 472, 514
BstMBI GATC 4 cut(s) 64, 391, 469, 511
BstMWI GCNNNNNNNGC 2 cut(s) 42, 165
BstNSI RCATGY 2 cut(s) 362, 595
BstSFI CTRYAG 2 cut(s) 249, 639
BstSLI GKGCMC 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 683
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuRI GGCC 5 cut(s) 87, 319, 343, 385, 424
BtgZI GCGATG 1 cut(s) 768
BtsCI GGATG 3 cut(s) 10, 398, 506
BtsIMutI CAGTG 1 cut(s) 262
BveI ACCTGC 2 cut(s) 448, 487
Cac8I GCNNGC 1 cut(s) 279
CfoI GCGC 1 cut(s) 780
Cfr13I GGNCC 2 cut(s) 350, 431
Csp6I GTAC 2 cut(s) 21, 589
CviAII CATG 4 cut(s) 117, 137, 359, 592
CviQI GTAC 2 cut(s) 21, 589
DdeI CTNAG 1 cut(s) 801
DpnI GATC 4 cut(s) 66, 393, 471, 513
DpnII GATC 4 cut(s) 64, 391, 469, 511
DraI TTTAAA 1 cut(s) 231
DraIII CACNNNGTG 1 cut(s) 83
EaeI YGGCCR 4 cut(s) 85, 341, 383, 422
Eam1104I CTCTTC 1 cut(s) 672
EarI CTCTTC 1 cut(s) 672
Eco24I GRGCYC 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 350, 431
Eco57I CTGAAG 4 cut(s) 288, 312, 354, 435
EcoT38I GRGCYC 1 cut(s) 269
FaeI CATG 4 cut(s) 120, 140, 362, 595
FaqI GGGAC 1 cut(s) 597
FatI CATG 4 cut(s) 116, 136, 358, 591
FauNDI CATATG 1 cut(s) 461
Fnu4HI GCNGC 1 cut(s) 672
FokI GGATG 3 cut(s) 17, 385, 493
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 672
FspBI CTAG 1 cut(s) 596
GlaI GCGC 1 cut(s) 779
GluI GCNGC 1 cut(s) 672
GsaI CCCAGC 1 cut(s) 784
GsuI CTGGAG 2 cut(s) 318, 360
HaeII RGCGCY 1 cut(s) 781
HaeIII GGCC 5 cut(s) 87, 319, 343, 385, 424
HhaI GCGC 1 cut(s) 780
Hin1II CATG 4 cut(s) 120, 140, 362, 595
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HinfI GANTC 5 cut(s) 27, 50, 140, 196, 659
HphI GGTGA 1 cut(s) 23
Hpy188I TCNGA 2 cut(s) 511, 552
Hpy188III TCNNGA 3 cut(s) 335, 377, 416
HpyAV CCTTC 6 cut(s) 336, 417, 459, 540, 695, 755
HpyCH4III ACNGT 3 cut(s) 503, 588, 721
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 3 cut(s) 36, 99, 168
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 165
HpyF3I CTNAG 1 cut(s) 801
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 4 cut(s) 120, 140, 362, 595
HspAI GCGC 1 cut(s) 778
Kzo9I GATC 4 cut(s) 64, 391, 469, 511
LmnI GCTCC 5 cut(s) 313, 337, 379, 418, 460
Lsp1109I GCAGC 1 cut(s) 683
MaeI CTAG 1 cut(s) 596
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 2 cut(s) 73, 742
MalI GATC 4 cut(s) 66, 393, 471, 513
MboI GATC 4 cut(s) 64, 391, 469, 511
MboII GAAGA 3 cut(s) 360, 689, 710
MfeI CAATTG 1 cut(s) 69
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 3 cut(s) 155, 269, 792
MlsI TGGCCA 2 cut(s) 87, 385
MluCI AATT 1 cut(s) 69
MluNI TGGCCA 2 cut(s) 87, 385
MlyI GAGTC 1 cut(s) 668
MmeI TCCRAC 1 cut(s) 419
MnlI CCTC 8 cut(s) 141, 175, 210, 594, 661, 675, 724, 727
Mox20I TGGCCA 2 cut(s) 87, 385
MscI TGGCCA 2 cut(s) 87, 385
MseI TTAA 3 cut(s) 230, 572, 693
MslI CAYNNNNRTG 1 cut(s) 596
Msp20I TGGCCA 2 cut(s) 87, 385
MunI CAATTG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 42, 165
NdeI CATATG 1 cut(s) 461
NdeII GATC 4 cut(s) 64, 391, 469, 511
NlaIII CATG 4 cut(s) 120, 140, 362, 595
NlaIV GGNNCC 2 cut(s) 152, 414
NmuCI GTSAC 1 cut(s) 742
NspI RCATGY 2 cut(s) 362, 595
PfeI GAWTC 4 cut(s) 27, 50, 140, 196
PkrI GCNGC 1 cut(s) 673
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
Ppu21I YACGTR 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 780
PspN4I GGNNCC 2 cut(s) 152, 414
PspPI GGNCC 2 cut(s) 350, 431
PsuI RGATCY 1 cut(s) 64
RsaI GTAC 2 cut(s) 22, 590
RsaNI GTAC 2 cut(s) 21, 589
RseI CAYNNNNRTG 1 cut(s) 596
SaqAI TTAA 3 cut(s) 230, 572, 693
SatI GCNGC 1 cut(s) 672
Sau3AI GATC 4 cut(s) 64, 391, 469, 511
Sau96I GGNCC 2 cut(s) 350, 431
SchI GAGTC 1 cut(s) 668
SduI GDGCHC 3 cut(s) 155, 269, 792
SfcI CTRYAG 2 cut(s) 249, 639
SinI GGWCC 2 cut(s) 350, 431
SmiI ATTTAAAT 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 596
Sse9I AATT 1 cut(s) 69
SspMI CTAG 1 cut(s) 596
SwaI ATTTAAAT 1 cut(s) 231
TaaI ACNGT 3 cut(s) 503, 588, 721
TaiI ACGT 1 cut(s) 580
TaqI TCGA 1 cut(s) 662
TasI AATT 1 cut(s) 69
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 4 cut(s) 27, 50, 140, 196
Tru1I TTAA 3 cut(s) 230, 572, 693
Tru9I TTAA 3 cut(s) 230, 572, 693
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 742
TseI GCWGC 1 cut(s) 671
Tsp45I GTSAC 1 cut(s) 742
TspDTI ATGAA 3 cut(s) 113, 495, 711
TspGWI ACGGA 1 cut(s) 761
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 350, 431
XceI RCATGY 2 cut(s) 362, 595
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.