MD10G1270900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007491
Physical Location & Seq
Forward (+)
36308235 .. 36313635
5401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1270900.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGTTATCACAATCTCCTCCTGTTTATGGAGAGCCACAAGTATCTTGTTTCTTCATTTTTGGCGATTCATTGGCCGACAATGGCAATAATAACCTCCTCCCGACATCTGCTATAGTTAACTACAAGCCCTACGGGATTGATTTTCCCGTTGAACCATCTGGAAGATTTTGCAATGGCCGCACCACTCTTTTTCTTTCACAATGGGAACAAATGACAGCAACATGGACGTTGATGATGATTAATGTTGTTTTGGTGATTTCACTATTACAATCTTGCGTTTGTGGAGAACCAAAGGTGCCCTGTTTCTTCATTTTTGGTGATTCATTGGCAGATAATGGCAATAATAACCGCCTCCAGACCCCGTCTAGAGGGAACTACAAGCCTTACGGGATTGATTTTCCCCAGGGACCAACAGGGAGGTTTACCAATGGCCGAACCATTGTTGATATTTTAGCTGAACTTCTGGGTTTTGAGAATCCCATCCCATGCTTTGCCAATACTAGGGGGTACAAGAACATTGTTAGAGGTCTGAATTATGCATCTGCCTCAGCAGGACTTCGAGATGAAACTGGCTCCCATATGGGTGAGAATGTCAATTTAAATAAGCAATTACTGAATCACAAATCCACAGTCAAGCGCATTACTTCAATAATGGGTCTTAAACAATTGTCCAGGCAGCATTTACAAAAGTGCTTATATTCAGTTGGAATGGGCAGCAACGATTATCTTATGAATTACTTCCAGCCCAAGTTTTATCCCACCAGTAAGCAATACACCCTTGAACAATATGCTTCAACTCTGTACAAGTATGGAGCAAGGAAGGTGGTCTTGAATGGAGGACTAGTAGGATGCACTCCATCTTCAATTTCGACTACAAATGGGTCTACATGCGCAGAAAATTTGAACAATGCAGTTCAGTTTTTTAACCAAAAGCTTAAGTCACTCGTTGATCAGCTCAACGCCAAACTGACCGATGCAAAATTCATCTATGTTGACATGTTTGGAATTTCCGGAAGTCCATCAGATCTTACAGCTGCAGGATTCAAGGTGACTAAAGCAAACTGTTGTCCGGTAGATGAATCTGGCCAATGTCTTCCTTCCAAAGCTCCATGCCAGAATAGAAGCGAGTATATGTTCTGGGATTTATTCCATCCTACTGAAGCTTCTAATCTCATCACTGCAAGAAAGACATACAATTCTTCTAATTCGTCGGACACTTATCCAATGAATCTTAGTCACCTAATTCAGCTTAGGCTATAA

Protein Analysis

420

Amino Acids

46.37

Weight (kDa)

8.68

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 103 - 395 8.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 892
AccB1I GGYRCC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 884
AccIII TCCGGA 1 cut(s) 1010
AciI CCGC 2 cut(s) 178, 349
AcoI YGGCCR 4 cut(s) 72, 175, 430, 1084
AcsI RAATTY 3 cut(s) 898, 980, 1005
AcuI CTGAAG 1 cut(s) 1179
AfaI GTAC 2 cut(s) 509, 803
AfiI CCNNNNNNNGG 3 cut(s) 26, 368, 501
AflII CTTAAG 1 cut(s) 935
AflIII ACRYGT 1 cut(s) 996
AgsI TTSAA 8 cut(s) 152, 648, 782, 795, 832, 864, 904, 1045
AhlI ACTAGT 1 cut(s) 841
AjnI CCWGG 2 cut(s) 402, 671
AluBI AGCT 7 cut(s) 455, 934, 955, 1034, 1106, 1163, 1249
AluI AGCT 7 cut(s) 455, 934, 955, 1034, 1106, 1163, 1249
Aor13HI TCCGGA 1 cut(s) 1010
AoxI GGCC 4 cut(s) 72, 175, 430, 1084
ApeKI GCWGC 3 cut(s) 676, 714, 1034
ApoI RAATTY 3 cut(s) 898, 980, 1005
ArsI GACNNNNNNTTYG 2 cut(s) 615, 647
AseI ATTAAT 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 737
AspLEI GCGC 2 cut(s) 639, 893
AspS9I GGNCC 1 cut(s) 407
AsuHPI GGTGA 5 cut(s) 265, 329, 596, 1060, 1229
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 300
BalI TGGCCA 1 cut(s) 1086
BanI GGYRCC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 1085
BbvCI CCTCAGC 1 cut(s) 547
BbvI GCAGC 3 cut(s) 688, 726, 1021
BccI CCATC 5 cut(s) 163, 488, 865, 1027, 1158
BciT130I CCWGG 2 cut(s) 404, 673
BclI TGATCA 1 cut(s) 949
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 3 cut(s) 366, 501, 842
BfmI CTRYAG 2 cut(s) 111, 1035
BfrI CTTAAG 1 cut(s) 935
BglII AGATCT 1 cut(s) 1024
BisI GCNGC 4 cut(s) 178, 677, 715, 1035
BlsI GCNGC 4 cut(s) 179, 678, 716, 1036
Bme1390I CCNGG 2 cut(s) 404, 673
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 3 cut(s) 297, 408, 574
BmrFI CCNGG 2 cut(s) 404, 673
BmsI GCATC 3 cut(s) 548, 839, 964
BpiI GAAGAC 1 cut(s) 1085
BpmI CTGGAG 1 cut(s) 338
Bpu10I CCTNAGC 2 cut(s) 547, 1250
BsaJI CCNNGG 2 cut(s) 402, 403
BsaWI WCCGGW 2 cut(s) 1010, 1069
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 368, 501
Bse1I ACTGG 2 cut(s) 574, 762
Bse3DI GCAATG 1 cut(s) 178
BseAI TCCGGA 1 cut(s) 1010
BseBI CCWGG 2 cut(s) 404, 673
BseDI CCNNGG 2 cut(s) 402, 403
BseGI GGATG 3 cut(s) 480, 854, 1150
BseLI CCNNNNNNNGG 3 cut(s) 26, 368, 501
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 561
BseNI ACTGG 2 cut(s) 574, 762
BseRI GAGGAG 2 cut(s) 6, 86
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 3 cut(s) 688, 726, 1021
BshFI GGCC 4 cut(s) 74, 177, 432, 1086
BshNI GGYRCC 1 cut(s) 295
BsiSI CCGG 2 cut(s) 1011, 1070
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 3 cut(s) 26, 368, 501
BsmFI GGGAC 1 cut(s) 420
BsnI GGCC 4 cut(s) 74, 177, 432, 1086
Bsp1286I GDGCHC 1 cut(s) 300
Bsp13I TCCGGA 1 cut(s) 1010
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 2 cut(s) 949, 1024
BspACI CCGC 2 cut(s) 178, 349
BspANI GGCC 4 cut(s) 74, 177, 432, 1086
BspCNI CTCAG 1 cut(s) 560
BspEI TCCGGA 1 cut(s) 1010
BspLI GGNNCC 3 cut(s) 297, 408, 574
BspMAI CTGCAG 1 cut(s) 1039
BspT107I GGYRCC 1 cut(s) 295
BspTI CTTAAG 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 178
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 2 cut(s) 574, 762
BssECI CCNNGG 2 cut(s) 402, 403
BssMI GATC 2 cut(s) 949, 1024
Bst2UI CCWGG 2 cut(s) 404, 673
Bst4CI ACNGT 2 cut(s) 631, 1064
BstAFI CTTAAG 1 cut(s) 935
BstAUI TGTACA 1 cut(s) 801
BstDEI CTNAG 3 cut(s) 547, 1232, 1250
BstF5I GGATG 3 cut(s) 480, 854, 1150
BstHHI GCGC 2 cut(s) 639, 893
BstKTI GATC 2 cut(s) 952, 1027
BstMBI GATC 2 cut(s) 949, 1024
BstMWI GCNNNNNNNGC 1 cut(s) 177
BstNI CCWGG 2 cut(s) 404, 673
BstNSI RCATGY 2 cut(s) 891, 1000
BstSCI CCNGG 2 cut(s) 402, 671
BstSFI CTRYAG 2 cut(s) 111, 1035
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 3 cut(s) 688, 726, 1021
BstV2I GAAGAC 1 cut(s) 1085
BstX2I RGATCY 1 cut(s) 1024
BstYI RGATCY 1 cut(s) 1024
BsuRI GGCC 4 cut(s) 74, 177, 432, 1086
BtsCI GGATG 3 cut(s) 480, 854, 1150
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 1 cut(s) 1176
CfoI GCGC 2 cut(s) 639, 893
Cfr13I GGNCC 1 cut(s) 407
Csp6I GTAC 2 cut(s) 508, 802
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 5 cut(s) 222, 486, 888, 997, 1110
CviQI GTAC 2 cut(s) 508, 802
DdeI CTNAG 3 cut(s) 547, 1232, 1250
DpnI GATC 2 cut(s) 951, 1026
DpnII GATC 2 cut(s) 949, 1024
DraI TTTAAA 1 cut(s) 600
EaeI YGGCCR 4 cut(s) 72, 175, 430, 1084
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 1 cut(s) 1179
EcoRII CCWGG 2 cut(s) 402, 671
EcoT22I ATGCAT 1 cut(s) 541
FaeI CATG 5 cut(s) 225, 489, 891, 1000, 1113
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 1 cut(s) 420
FatI CATG 5 cut(s) 221, 485, 887, 996, 1109
FauNDI CATATG 1 cut(s) 579
FbaI TGATCA 1 cut(s) 949
FblI GTMKAC 1 cut(s) 884
Fnu4HI GCNGC 4 cut(s) 178, 677, 715, 1035
FokI GGATG 3 cut(s) 467, 861, 1137
Fsp4HI GCNGC 4 cut(s) 178, 677, 715, 1035
FspBI CTAG 3 cut(s) 366, 501, 842
FspI TGCGCA 1 cut(s) 892
GlaI GCGC 2 cut(s) 638, 892
GluI GCNGC 4 cut(s) 178, 677, 715, 1035
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 4 cut(s) 74, 177, 432, 1086
HapII CCGG 2 cut(s) 1011, 1070
HhaI GCGC 2 cut(s) 639, 893
Hin1II CATG 5 cut(s) 225, 489, 891, 1000, 1113
Hin6I GCGC 2 cut(s) 637, 891
HinP1I GCGC 2 cut(s) 637, 891
HincII GTYRAC 2 cut(s) 118, 994
HindII GTYRAC 2 cut(s) 118, 994
HindIII AAGCTT 2 cut(s) 932, 1161
HinfI GANTC 7 cut(s) 65, 320, 475, 616, 1041, 1079, 1228
HpaI GTTAAC 1 cut(s) 118
HpaII CCGG 2 cut(s) 1011, 1070
HphI GGTGA 5 cut(s) 265, 329, 596, 1060, 1229
Hpy166II GTNNAC 4 cut(s) 118, 423, 885, 994
Hpy188I TCNGA 3 cut(s) 531, 1024, 1213
Hpy188III TCNNGA 7 cut(s) 100, 159, 355, 366, 560, 829, 1011
Hpy8I GTNNAC 4 cut(s) 118, 423, 885, 994
Hpy99I CGWCG 1 cut(s) 1213
HpyAV CCTTC 2 cut(s) 814, 1107
HpyCH4III ACNGT 2 cut(s) 631, 1064
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 7 cut(s) 171, 539, 852, 911, 977, 1037, 1181
HpyF10VI GCNNNNNNNGC 1 cut(s) 177
HpyF3I CTNAG 3 cut(s) 547, 1232, 1250
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 5 cut(s) 225, 489, 891, 1000, 1113
HspAI GCGC 2 cut(s) 637, 891
Kpn2I TCCGGA 1 cut(s) 1010
Ksp22I TGATCA 1 cut(s) 949
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 949, 1024
LmnI GCTCC 3 cut(s) 578, 812, 1111
Lsp1109I GCAGC 3 cut(s) 688, 726, 1021
LweI GCATC 3 cut(s) 548, 839, 964
MaeI CTAG 3 cut(s) 366, 501, 842
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 3 cut(s) 939, 1048, 1235
MalI GATC 2 cut(s) 951, 1026
MboI GATC 2 cut(s) 949, 1024
MboII GAAGA 6 cut(s) 43, 174, 298, 852, 1085, 1191
MfeI CAATTG 1 cut(s) 665
MflI RGATCY 1 cut(s) 1024
MhlI GDGCHC 1 cut(s) 300
MlsI TGGCCA 1 cut(s) 1086
MluNI TGGCCA 1 cut(s) 1086
MmeI TCCRAC 2 cut(s) 685, 1191
MnlI CCTC 9 cut(s) 27, 104, 107, 362, 362, 411, 518, 556, 830
Mox20I TGGCCA 1 cut(s) 1086
Mph1103I ATGCAT 1 cut(s) 541
MroI TCCGGA 1 cut(s) 1010
MroXI GAANNNNTTC 1 cut(s) 737
MscI TGGCCA 1 cut(s) 1086
MseI TTAA 6 cut(s) 117, 240, 599, 660, 924, 936
MslI CAYNNNNRTG 1 cut(s) 582
Msp20I TGGCCA 1 cut(s) 1086
MspA1I CMGCKG 1 cut(s) 1034
MspCI CTTAAG 1 cut(s) 935
MspI CCGG 2 cut(s) 1011, 1070
MspR9I CCNGG 2 cut(s) 404, 673
MunI CAATTG 1 cut(s) 665
MvaI CCWGG 2 cut(s) 404, 673
MwoI GCNNNNNNNGC 1 cut(s) 177
NdeI CATATG 1 cut(s) 579
NdeII GATC 2 cut(s) 949, 1024
NlaIII CATG 5 cut(s) 225, 489, 891, 1000, 1113
NlaIV GGNNCC 3 cut(s) 297, 408, 574
NmuCI GTSAC 3 cut(s) 939, 1048, 1235
NsbI TGCGCA 1 cut(s) 892
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 2 cut(s) 891, 1000
PasI CCCWGGG 1 cut(s) 403
PciI ACATGT 1 cut(s) 996
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 7 cut(s) 65, 320, 475, 616, 1041, 1079, 1228
PflFI GACNNNGTC 1 cut(s) 361
PkrI GCNGC 4 cut(s) 179, 678, 716, 1036
PscI ACATGT 1 cut(s) 996
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 402, 671
PspGI CCWGG 2 cut(s) 402, 671
PspN4I GGNNCC 3 cut(s) 297, 408, 574
PspPI GGNCC 1 cut(s) 407
PstI CTGCAG 1 cut(s) 1039
PsuI RGATCY 1 cut(s) 1024
PsyI GACNNNGTC 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 2 cut(s) 509, 803
RsaNI GTAC 2 cut(s) 508, 802
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 6 cut(s) 117, 240, 599, 660, 924, 936
SatI GCNGC 4 cut(s) 178, 677, 715, 1035
Sau3AI GATC 2 cut(s) 949, 1024
Sau96I GGNCC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 404, 673
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 3 cut(s) 548, 839, 964
SfcI CTRYAG 2 cut(s) 111, 1035
SinI GGWCC 1 cut(s) 407
SmiI ATTTAAAT 1 cut(s) 600
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
SpeI ACTAGT 1 cut(s) 841
SsiI CCGC 2 cut(s) 178, 349
SspMI CTAG 3 cut(s) 366, 501, 842
StyD4I CCNGG 2 cut(s) 402, 671
SwaI ATTTAAAT 1 cut(s) 600
TaaI ACNGT 2 cut(s) 631, 1064
TaiI ACGT 1 cut(s) 230
TaqI TCGA 2 cut(s) 559, 869
TaqII GACCGA 1 cut(s) 986
TatI WGTACW 1 cut(s) 801
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 7 cut(s) 65, 320, 475, 616, 1041, 1079, 1228
Tru1I TTAA 6 cut(s) 117, 240, 599, 660, 924, 936
Tru9I TTAA 6 cut(s) 117, 240, 599, 660, 924, 936
TscAI CASTG 1 cut(s) 1183
TseFI GTSAC 3 cut(s) 939, 1048, 1235
TseI GCWGC 3 cut(s) 676, 714, 1034
Tsp45I GTSAC 3 cut(s) 939, 1048, 1235
TspDTI ATGAA 9 cut(s) 43, 57, 298, 312, 579, 746, 973, 1092, 1241
TspRI CASTG 1 cut(s) 1183
Tth111I GACNNNGTC 1 cut(s) 361
Vha464I CTTAAG 1 cut(s) 935
VpaK11BI GGWCC 1 cut(s) 407
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 3 cut(s) 898, 980, 1005
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 2 cut(s) 891, 1000
XmiI GTMKAC 1 cut(s) 884
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 3 cut(s) 366, 501, 842
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.