RchiOBHm_Chr5g0012441

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007491
Physical Location & Seq
Reverse (-)
8357101 .. 8359193
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29301

Sequence Viewer

Length: 1089 bp
ATGACAAAAGATGCACTGAAATGGTTAATGGTGATTGCTGTTTTGGTGGCTTCAATATCACAATCCTGTGTTCATGGAGCGCCACAAGTACCTTGCTTCTTCATTTTTGGTGATTCAATGGCCGATAACGGCAATAACAACCTCCTATTGACTTCGGCCAAAGTGAACTATGCGCCGTACGGAATTGATTATCCCGGTGGAGTAACAACAGGAAGATTTTCCAATGGCAAAACTACTGTTGATATACTAGCTGAATTTCTGGGGTTTGAGACTCCCATCCCACCATTTGCAACTACCAACGTACAGGACATTGTTAAAGGTCTCAATTATGCATCTGGCGCCGCAGGGATTCTAACAGAAACTGGCTCCAATTTGGGTGGTCACATCAGCTTAGACCAGCAGTTACTGAATCACCAAATCACAGTCTTGCGCATTGCTTCAATACTTCGAGCAAATACATTACAAACCTTGCAGTACTTGAACAAGTGTCTATACACAGTGGGAATGGGTAACAATGATTACATTAACAATTACTTCATGCCTCAGTTTTATCCCACCAGCAAGAGATACACCCCTGAACAATATGCTTCAATTCTTATCGACCAATATACTACCCAAATACTGACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACTAATAGGTTGCACTCCTAATGCAATTTCGAAAACGAGGGGGACCACATGTGTAGATGAATTGAACAGTGCCGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTCAATACCAATTTGACTGATGCAAAATTCATCTATGTCAACAGTACTGGAATGAGTTCCGGGGATCCTACATCTGTTGGATTCAAGGTTTTAAATGTTGGGTGTTGTCCGGTGAATAGTGTTGGCCAATGTATTCGTTCCGGAACTCCATGCCAGAATAGGAGTGAGTATGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACCGAATTACTGCATTCAGAACATACAAAAGTGTTGACCCCACGGATAATTATCCTATGGATGTCAGTCACTTAGTTCAGCTTAAACTATAA

Protein Analysis

362

Amino Acids

39.53

Weight (kDa)

7.51

Isoelectric Point (pI)

27.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 337 1.7e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 338
AccIII TCCGGA 1 cut(s) 929
AciI CCGC 2 cut(s) 342, 747
AclWI GGATC 2 cut(s) 848, 861
AcoI YGGCCR 3 cut(s) 120, 156, 913
AcsI RAATTY 2 cut(s) 254, 815
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 7 cut(s) 90, 179, 303, 476, 632, 774, 835
AfiI CCNNNNNNNGG 1 cut(s) 988
AflIII ACRYGT 1 cut(s) 722
AgsI TTSAA 8 cut(s) 54, 117, 441, 481, 591, 739, 760, 874
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AluBI AGCT 6 cut(s) 251, 390, 754, 769, 790, 1078
AluI AGCT 6 cut(s) 251, 390, 754, 769, 790, 1078
Alw26I GTCTC 2 cut(s) 263, 326
AlwI GGATC 2 cut(s) 848, 861
AlwNI CAGNNNCTG 2 cut(s) 362, 406
Aor13HI TCCGGA 1 cut(s) 929
AoxI GGCC 3 cut(s) 120, 156, 913
ApoI RAATTY 2 cut(s) 254, 815
Asp700I GAANNNNTTC 2 cut(s) 217, 844
AspLEI GCGC 4 cut(s) 82, 175, 341, 432
AspS9I GGNCC 1 cut(s) 717
AsuC2I CCSGG 2 cut(s) 195, 850
AsuHPI GGTGA 4 cut(s) 43, 122, 404, 913
AsuII TTCGAA 1 cut(s) 704
AvaII GGWCC 1 cut(s) 717
BalI TGGCCA 1 cut(s) 915
BamHI GGATCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 338
BccI CCATC 1 cut(s) 284
BceAI ACGGC 2 cut(s) 145, 160
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 2 cut(s) 195, 850
BcoDI GTCTC 2 cut(s) 263, 326
BfaI CTAG 1 cut(s) 248
BfoI RGCGCY 2 cut(s) 83, 342
BisI GCNGC 2 cut(s) 342, 747
BlsI GCNGC 2 cut(s) 343, 748
BmcAI AGTACT 2 cut(s) 476, 835
Bme1390I CCNGG 2 cut(s) 195, 850
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 4 cut(s) 340, 367, 718, 855
BmrFI CCNGG 2 cut(s) 195, 850
BmrI ACTGGG 1 cut(s) 680
BmsI GCATC 3 cut(s) 341, 799, 1001
BmuI ACTGGG 1 cut(s) 680
Bpu14I TTCGAA 1 cut(s) 704
BpuMI CCSGG 2 cut(s) 195, 850
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 660, 849, 1038
BsaWI WCCGGW 2 cut(s) 898, 929
Bsc4I CCNNNNNNNGG 1 cut(s) 988
Bse1I ACTGG 3 cut(s) 367, 675, 841
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 929
BseDI CCNNGG 3 cut(s) 660, 849, 1038
BseGI GGATG 2 cut(s) 276, 1063
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 1 cut(s) 988
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 2 cut(s) 557, 978
BseNI ACTGG 3 cut(s) 367, 675, 841
BseYI CCCAGC 1 cut(s) 750
BshFI GGCC 3 cut(s) 122, 158, 915
BshNI GGYRCC 1 cut(s) 338
BsiSI CCGG 4 cut(s) 195, 849, 899, 930
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 3 cut(s) 687, 730, 983
BslI CCNNNNNNNGG 1 cut(s) 988
BsmAI GTCTC 2 cut(s) 263, 326
BsmFI GGGAC 3 cut(s) 687, 730, 983
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 3 cut(s) 122, 158, 915
Bso31I GGTCTC 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 704
Bsp13I TCCGGA 1 cut(s) 929
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 2 cut(s) 784, 853
BspACI CCGC 2 cut(s) 342, 747
BspANI GGCC 3 cut(s) 122, 158, 915
BspCNI CTCAG 2 cut(s) 556, 979
BspEI TCCGGA 1 cut(s) 929
BspLI GGNNCC 4 cut(s) 340, 367, 718, 855
BspPI GGATC 2 cut(s) 848, 861
BspT104I TTCGAA 1 cut(s) 704
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 432
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 3 cut(s) 367, 675, 841
BssECI CCNNGG 3 cut(s) 660, 849, 1038
BssMI GATC 2 cut(s) 784, 853
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 660
Bst4CI ACNGT 5 cut(s) 238, 424, 499, 743, 833
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 630
BstBI TTCGAA 1 cut(s) 704
BstDEI CTNAG 4 cut(s) 391, 543, 987, 1069
BstDSI CCRYGG 1 cut(s) 1038
BstF5I GGATG 2 cut(s) 276, 1063
BstH2I RGCGCY 2 cut(s) 83, 342
BstHHI GCGC 4 cut(s) 82, 175, 341, 432
BstKTI GATC 2 cut(s) 787, 856
BstMAI GTCTC 2 cut(s) 263, 326
BstMBI GATC 2 cut(s) 784, 853
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 2 cut(s) 193, 848
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuRI GGCC 3 cut(s) 122, 158, 915
BtgI CCRYGG 1 cut(s) 1038
BtsCI GGATG 2 cut(s) 276, 1063
BtsIMutI CAGTG 3 cut(s) 14, 504, 748
CaiI CAGNNNCTG 2 cut(s) 362, 406
CfoI GCGC 4 cut(s) 82, 175, 341, 432
Cfr13I GGNCC 1 cut(s) 717
Csp6I GTAC 7 cut(s) 89, 178, 302, 475, 631, 773, 834
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 4 cut(s) 74, 538, 723, 939
CviQI GTAC 7 cut(s) 89, 178, 302, 475, 631, 773, 834
DdeI CTNAG 4 cut(s) 391, 543, 987, 1069
DinI GGCGCC 1 cut(s) 340
DpnI GATC 2 cut(s) 786, 855
DpnII GATC 2 cut(s) 784, 853
DraI TTTAAA 1 cut(s) 882
EaeI YGGCCR 3 cut(s) 120, 156, 913
Eco130I CCWWGG 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 717
EcoT14I CCWWGG 1 cut(s) 660
EcoT22I ATGCAT 1 cut(s) 334
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 4 cut(s) 77, 541, 726, 942
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 3 cut(s) 687, 730, 983
FatI CATG 4 cut(s) 73, 537, 722, 938
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 2 cut(s) 342, 747
FokI GGATG 2 cut(s) 263, 1070
Fsp4HI GCNGC 2 cut(s) 342, 747
FspBI CTAG 1 cut(s) 248
FspI TGCGCA 1 cut(s) 431
GlaI GCGC 4 cut(s) 81, 174, 340, 431
GluI GCNGC 2 cut(s) 342, 747
GsaI CCCAGC 1 cut(s) 754
HaeII RGCGCY 2 cut(s) 83, 342
HaeIII GGCC 3 cut(s) 122, 158, 915
HapII CCGG 4 cut(s) 195, 849, 899, 930
HhaI GCGC 4 cut(s) 82, 175, 341, 432
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 4 cut(s) 77, 541, 726, 942
Hin6I GCGC 4 cut(s) 80, 173, 339, 430
HinP1I GCGC 4 cut(s) 80, 173, 339, 430
HincII GTYRAC 2 cut(s) 829, 1033
HindII GTYRAC 2 cut(s) 829, 1033
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 6 cut(s) 113, 271, 349, 409, 870, 971
HpaII CCGG 4 cut(s) 195, 849, 899, 930
HphI GGTGA 4 cut(s) 43, 122, 404, 913
Hpy166II GTNNAC 3 cut(s) 166, 829, 1033
Hpy188I TCNGA 1 cut(s) 1016
Hpy188III TCNNGA 1 cut(s) 930
Hpy8I GTNNAC 3 cut(s) 166, 829, 1033
HpyAV CCTTC 1 cut(s) 646
HpyCH4III ACNGT 5 cut(s) 238, 424, 499, 743, 833
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 8 cut(s) 14, 290, 332, 472, 687, 698, 812, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 4 cut(s) 391, 543, 987, 1069
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 4 cut(s) 77, 541, 726, 942
HspAI GCGC 4 cut(s) 80, 173, 339, 430
KasI GGCGCC 1 cut(s) 338
Kpn2I TCCGGA 1 cut(s) 929
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 2 cut(s) 784, 853
LmnI GCTCC 3 cut(s) 77, 371, 644
LweI GCATC 3 cut(s) 341, 799, 1001
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 5 cut(s) 202, 380, 402, 509, 1064
MalI GATC 2 cut(s) 786, 855
MboI GATC 2 cut(s) 784, 853
MboII GAAGA 2 cut(s) 91, 225
MflI RGATCY 1 cut(s) 853
MlsI TGGCCA 1 cut(s) 915
MluNI TGGCCA 1 cut(s) 915
Mly113I GGCGCC 1 cut(s) 339
MlyI GAGTC 2 cut(s) 265, 965
MmeI TCCRAC 2 cut(s) 646, 847
MnlI CCTC 5 cut(s) 152, 552, 705, 786, 982
Mox20I TGGCCA 1 cut(s) 915
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 929
MroXI GAANNNNTTC 2 cut(s) 217, 844
MscI TGGCCA 1 cut(s) 915
MseI TTAA 5 cut(s) 26, 315, 525, 881, 1080
MslI CAYNNNNRTG 1 cut(s) 19
Msp20I TGGCCA 1 cut(s) 915
MspI CCGG 4 cut(s) 195, 849, 899, 930
MspR9I CCNGG 2 cut(s) 195, 850
Mva1269I GAATGC 1 cut(s) 1010
MwoI GCNNNNNNNGC 1 cut(s) 338
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 2 cut(s) 195, 850
NdeII GATC 2 cut(s) 784, 853
NlaIII CATG 4 cut(s) 77, 541, 726, 942
NlaIV GGNNCC 4 cut(s) 340, 367, 718, 855
NmuCI GTSAC 2 cut(s) 380, 1064
NsbI TGCGCA 1 cut(s) 431
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 726
NspV TTCGAA 1 cut(s) 704
PciI ACATGT 1 cut(s) 722
PctI GAATGC 1 cut(s) 1010
PdmI GAANNNNTTC 2 cut(s) 217, 844
PfeI GAWTC 4 cut(s) 113, 349, 409, 870
Pfl23II CGTACG 1 cut(s) 177
PkrI GCNGC 2 cut(s) 343, 748
PleI GAGTC 2 cut(s) 265, 965
PluTI GGCGCC 1 cut(s) 342
PpsI GAGTC 2 cut(s) 265, 965
PscI ACATGT 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 750
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 4 cut(s) 340, 367, 718, 855
PspPI GGNCC 1 cut(s) 717
PstNI CAGNNNCTG 2 cut(s) 362, 406
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 7 cut(s) 90, 179, 303, 476, 632, 774, 835
RsaNI GTAC 7 cut(s) 89, 178, 302, 475, 631, 773, 834
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 5 cut(s) 26, 315, 525, 881, 1080
SatI GCNGC 2 cut(s) 342, 747
Sau3AI GATC 2 cut(s) 784, 853
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 2 cut(s) 476, 835
SchI GAGTC 2 cut(s) 265, 965
ScrFI CCNGG 2 cut(s) 195, 850
SfaNI GCATC 3 cut(s) 341, 799, 1001
SfoI GGCGCC 1 cut(s) 340
SfuI TTCGAA 1 cut(s) 704
SinI GGWCC 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 19
SsiI CCGC 2 cut(s) 342, 747
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 193, 848
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 5 cut(s) 238, 424, 499, 743, 833
TaiI ACGT 1 cut(s) 303
TaqI TCGA 4 cut(s) 448, 600, 704, 995
TatI WGTACW 3 cut(s) 474, 630, 833
TauI GCSGC 2 cut(s) 344, 749
TfiI GAWTC 4 cut(s) 113, 349, 409, 870
Tru1I TTAA 5 cut(s) 26, 315, 525, 881, 1080
Tru9I TTAA 5 cut(s) 26, 315, 525, 881, 1080
TscAI CASTG 3 cut(s) 21, 504, 748
TseFI GTSAC 2 cut(s) 380, 1064
Tsp45I GTSAC 2 cut(s) 380, 1064
TspDTI ATGAA 5 cut(s) 62, 91, 526, 747, 808
TspGWI ACGGA 2 cut(s) 195, 1055
TspRI CASTG 3 cut(s) 21, 504, 748
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 2 cut(s) 254, 815
XceI RCATGY 1 cut(s) 726
XmnI GAANNNNTTC 2 cut(s) 217, 844
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 2 cut(s) 476, 835
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.