RLG00000031916

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007491
Physical Location & Seq
Reverse (-)
9463382 .. 9472986
9605 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031916

Sequence Viewer

Length: 1092 bp
ATGACAAAAGATACACTGAAATGGTTAATGGTGATTGCTGTTTTGGTGGTTTCAATATCACAATCTTGTGTTCATGGAGCGCCACAAGTACCTTGCTTCTTCATTTTCGGTGATTCGTTGGCTGATAACGGCAATAACAACCTCCTCACGACTTTGGCCAAAGTGAACTACTCGCCGTACGGAATTGATTATCCAGGTGGAGTAAGAACAGGAAGATTTTCCAATGGCAAAACTACTGTTGATATACTGGGTCCTGAATTTCTGGGTTTTGAGATTCCCATCCCACCATTTGCAACTACCAAAGGACAGGACATTGTTAAAGGTCTCAATTATGCATCTGGCGCCGCAGGGATTCGAGCAGAAACTGGCTCCAATATGGGTGGTCACATCAGCTTAGATCAGCAGTTACTGAATCACCAAATCACAATCTTACGCATTGCTTCCATACTTCGAACAAATACAGTACAAACCTTGCAGTACTTGAACAAGTGCCTATATTCAGTCGGAATGGGTAGCAATGATTACTTTAACAATTACTTCATGCCTCAGTTTTATCCCACAAGCAAGAGATACACCCCTGAACAATATGCTTCAGTTCTTATTGACCAATATACTAGCCAAATACTGACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACTAATAGGTTGCACTCCTAACGCGATTTCGAAAACAAAGGGGACCGCATGTGTAGATGAATGGAACAGTGCGGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTAAACACCAATTTGACTGATGCAAAATTCATCTACGTCAACAGTACTGGAATGAGTTCAGGGGATCCTACATCTGTTGGATTCAAGGTTTTGAACGTTGGGTGTTGTCCGGTGAATAATGTTGGACAATGTGTTCGTTCCGGAACTCCATGCCAGAATAGGAGTGAGTACGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACCAAATCACTGCATTCAGAACATACAAAAGTGTTGACCCAACGGATAATTATCCTATGGATGTCAGTCACCTAGTTCAGCTTAAACCATAA

Protein Analysis

364

Amino Acids

39.74

Weight (kDa)

8.28

Isoelectric Point (pI)

27.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 338 1.1e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 341
AccII CGCG 1 cut(s) 701
AccIII TCCGGA 1 cut(s) 932
AciI CCGC 3 cut(s) 345, 723, 749
AclI AACGTT 1 cut(s) 888
AclWI GGATC 2 cut(s) 851, 864
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 257, 818
AcuI CTGAAG 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 8 cut(s) 90, 179, 465, 479, 635, 777, 838, 962
AfiI CCNNNNNNNGG 1 cut(s) 991
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 5 cut(s) 54, 484, 763, 877, 886
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AloI GAACNNNNNNTCC 2 cut(s) 909, 941
AluBI AGCT 5 cut(s) 393, 757, 772, 793, 1081
AluI AGCT 5 cut(s) 393, 757, 772, 793, 1081
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 2 cut(s) 851, 864
AlwNI CAGNNNCTG 2 cut(s) 365, 409
Aor13HI TCCGGA 1 cut(s) 932
AoxI GGCC 2 cut(s) 156, 750
ApoI RAATTY 2 cut(s) 257, 818
ArsI GACNNNNNNTTYG 2 cut(s) 909, 941
Asp700I GAANNNNTTC 2 cut(s) 217, 847
AspLEI GCGC 2 cut(s) 82, 344
AspS9I GGNCC 3 cut(s) 251, 720, 751
AsuHPI GGTGA 5 cut(s) 43, 122, 407, 916, 1061
AsuII TTCGAA 2 cut(s) 451, 707
AvaII GGWCC 2 cut(s) 251, 720
BalI TGGCCA 1 cut(s) 158
BamHI GGATCC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 341
BccI CCATC 1 cut(s) 287
BceAI ACGGC 2 cut(s) 145, 160
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 2 cut(s) 615, 1073
BfoI RGCGCY 2 cut(s) 83, 345
BisI GCNGC 2 cut(s) 345, 750
BlsI GCNGC 2 cut(s) 346, 751
BmcAI AGTACT 2 cut(s) 479, 838
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 2 cut(s) 251, 720
BmgT120I GGNCC 3 cut(s) 251, 720, 751
BmiI GGNNCC 5 cut(s) 252, 343, 370, 721, 858
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 2 cut(s) 257, 683
BmsI GCATC 3 cut(s) 344, 802, 1004
BmuI ACTGGG 2 cut(s) 257, 683
Bpu14I TTCGAA 2 cut(s) 451, 707
BsaAI YACGTR 1 cut(s) 964
BsaBI GATNNNNATC 2 cut(s) 278, 1050
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 663
BsaWI WCCGGW 2 cut(s) 901, 932
Bsc4I CCNNNNNNNGG 1 cut(s) 991
Bse1I ACTGG 4 cut(s) 252, 370, 678, 844
Bse3DI GCAATG 2 cut(s) 435, 523
Bse8I GATNNNNATC 2 cut(s) 278, 1050
BseAI TCCGGA 1 cut(s) 932
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 663
BseGI GGATG 2 cut(s) 279, 1066
BseJI GATNNNNATC 2 cut(s) 278, 1050
BseLI CCNNNNNNNGG 1 cut(s) 991
BseMI GCAATG 2 cut(s) 435, 523
BseMII CTCAG 2 cut(s) 560, 981
BseNI ACTGG 4 cut(s) 252, 370, 678, 844
BseRI GAGGAG 1 cut(s) 134
BseYI CCCAGC 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 701
BshFI GGCC 2 cut(s) 158, 752
BshNI GGYRCC 1 cut(s) 341
BsiSI CCGG 2 cut(s) 902, 933
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 3 cut(s) 690, 733, 986
BslI CCNNNNNNNGG 1 cut(s) 991
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 3 cut(s) 690, 733, 986
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 2 cut(s) 158, 752
Bso31I GGTCTC 1 cut(s) 329
Bsp119I TTCGAA 2 cut(s) 451, 707
Bsp13I TCCGGA 1 cut(s) 932
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 3 cut(s) 397, 787, 856
BspACI CCGC 3 cut(s) 345, 723, 749
BspANI GGCC 2 cut(s) 158, 752
BspCNI CTCAG 2 cut(s) 559, 982
BspEI TCCGGA 1 cut(s) 932
BspFNI CGCG 1 cut(s) 701
BspLI GGNNCC 5 cut(s) 252, 343, 370, 721, 858
BspPI GGATC 2 cut(s) 851, 864
BspT104I TTCGAA 2 cut(s) 451, 707
BspT107I GGYRCC 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 329
BsrDI GCAATG 2 cut(s) 435, 523
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 4 cut(s) 252, 370, 678, 844
BssECI CCNNGG 1 cut(s) 663
BssMI GATC 3 cut(s) 397, 787, 856
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 238, 463, 746, 836
BstACI GRCGYC 1 cut(s) 342
BstAUI TGTACA 1 cut(s) 633
BstBAI YACGTR 1 cut(s) 964
BstBI TTCGAA 2 cut(s) 451, 707
BstDEI CTNAG 3 cut(s) 394, 546, 990
BstF5I GGATG 2 cut(s) 279, 1066
BstFNI CGCG 1 cut(s) 701
BstH2I RGCGCY 2 cut(s) 83, 345
BstHHI GCGC 2 cut(s) 82, 344
BstKTI GATC 3 cut(s) 400, 790, 859
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 3 cut(s) 397, 787, 856
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 193
BstUI CGCG 1 cut(s) 701
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
BsuRI GGCC 2 cut(s) 158, 752
BtsCI GGATG 2 cut(s) 279, 1066
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 3 cut(s) 14, 751, 1008
CaiI CAGNNNCTG 2 cut(s) 365, 409
CfoI GCGC 2 cut(s) 82, 344
Cfr13I GGNCC 3 cut(s) 251, 720, 751
Csp6I GTAC 8 cut(s) 89, 178, 464, 478, 634, 776, 837, 961
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 4 cut(s) 74, 541, 726, 942
CviQI GTAC 8 cut(s) 89, 178, 464, 478, 634, 776, 837, 961
DdeI CTNAG 3 cut(s) 394, 546, 990
DinI GGCGCC 1 cut(s) 343
DpnI GATC 3 cut(s) 399, 789, 858
DpnII GATC 3 cut(s) 397, 787, 856
EaeI YGGCCR 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 663
Eco31I GGTCTC 1 cut(s) 329
Eco47I GGWCC 2 cut(s) 251, 720
Eco57I CTGAAG 1 cut(s) 576
EcoO109I RGGNCCY 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 663
EcoT22I ATGCAT 1 cut(s) 337
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 4 cut(s) 77, 544, 729, 945
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 3 cut(s) 690, 733, 986
FatI CATG 4 cut(s) 73, 540, 725, 941
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 345, 750
FokI GGATG 2 cut(s) 266, 1073
Fsp4HI GCNGC 2 cut(s) 345, 750
FspBI CTAG 2 cut(s) 615, 1073
GlaI GCGC 2 cut(s) 81, 343
GluI GCNGC 2 cut(s) 345, 750
GsaI CCCAGC 1 cut(s) 757
HaeII RGCGCY 2 cut(s) 83, 345
HaeIII GGCC 2 cut(s) 158, 752
HapII CCGG 2 cut(s) 902, 933
HhaI GCGC 2 cut(s) 82, 344
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 4 cut(s) 77, 544, 729, 945
Hin6I GCGC 2 cut(s) 80, 342
HinP1I GCGC 2 cut(s) 80, 342
HincII GTYRAC 2 cut(s) 832, 1036
HindII GTYRAC 2 cut(s) 832, 1036
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 6 cut(s) 113, 274, 352, 412, 873, 974
HpaII CCGG 2 cut(s) 902, 933
HphI GGTGA 5 cut(s) 43, 122, 407, 916, 1061
Hpy166II GTNNAC 3 cut(s) 166, 832, 1036
Hpy188I TCNGA 2 cut(s) 506, 1019
Hpy188III TCNNGA 3 cut(s) 148, 254, 933
Hpy8I GTNNAC 3 cut(s) 166, 832, 1036
HpyAV CCTTC 1 cut(s) 649
HpyCH4III ACNGT 4 cut(s) 238, 463, 746, 836
HpyCH4IV ACGT 3 cut(s) 828, 888, 963
HpyCH4V TGCA 6 cut(s) 293, 335, 475, 690, 815, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 3 cut(s) 394, 546, 990
HpySE526I ACGT 3 cut(s) 828, 888, 963
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 4 cut(s) 77, 544, 729, 945
HspAI GCGC 2 cut(s) 80, 342
KasI GGCGCC 1 cut(s) 341
Kpn2I TCCGGA 1 cut(s) 932
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 3 cut(s) 397, 787, 856
LmnI GCTCC 3 cut(s) 77, 374, 647
LweI GCATC 3 cut(s) 344, 802, 1004
MaeI CTAG 2 cut(s) 615, 1073
MaeII ACGT 3 cut(s) 828, 888, 963
MaeIII GTNAC 3 cut(s) 383, 405, 1067
MalI GATC 3 cut(s) 399, 789, 858
MboI GATC 3 cut(s) 397, 787, 856
MboII GAAGA 2 cut(s) 91, 225
MflI RGATCY 1 cut(s) 856
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 7 cut(s) 183, 257, 328, 532, 802, 818, 1048
MluNI TGGCCA 1 cut(s) 158
Mly113I GGCGCC 1 cut(s) 342
MlyI GAGTC 1 cut(s) 968
MmeI TCCRAC 4 cut(s) 484, 649, 850, 895
MnlI CCTC 5 cut(s) 152, 155, 555, 789, 985
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 337
MroI TCCGGA 1 cut(s) 932
MroXI GAANNNNTTC 2 cut(s) 217, 847
MscI TGGCCA 1 cut(s) 158
MseI TTAA 4 cut(s) 26, 318, 528, 1083
MslI CAYNNNNRTG 1 cut(s) 19
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 2 cut(s) 902, 933
MspR9I CCNGG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 701
MwoI GCNNNNNNNGC 1 cut(s) 341
NarI GGCGCC 1 cut(s) 342
NdeII GATC 3 cut(s) 397, 787, 856
NlaIII CATG 4 cut(s) 77, 544, 729, 945
NlaIV GGNNCC 5 cut(s) 252, 343, 370, 721, 858
NmuCI GTSAC 2 cut(s) 383, 1067
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 1 cut(s) 729
NspV TTCGAA 2 cut(s) 451, 707
PctI GAATGC 1 cut(s) 1013
PdmI GAANNNNTTC 2 cut(s) 217, 847
PfeI GAWTC 5 cut(s) 113, 274, 352, 412, 873
Pfl23II CGTACG 1 cut(s) 177
PkrI GCNGC 2 cut(s) 346, 751
PleI GAGTC 1 cut(s) 968
PluTI GGCGCC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 968
Ppu21I YACGTR 1 cut(s) 964
PpuMI RGGWCCY 1 cut(s) 251
Psp1406I AACGTT 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 753
PspGI CCWGG 1 cut(s) 193
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 5 cut(s) 252, 343, 370, 721, 858
PspPI GGNCC 3 cut(s) 251, 720, 751
PspPPI RGGWCCY 1 cut(s) 251
PstNI CAGNNNCTG 2 cut(s) 365, 409
PsuI RGATCY 1 cut(s) 856
RsaI GTAC 8 cut(s) 90, 179, 465, 479, 635, 777, 838, 962
RsaNI GTAC 8 cut(s) 89, 178, 464, 478, 634, 776, 837, 961
RseI CAYNNNNRTG 1 cut(s) 19
SaqAI TTAA 4 cut(s) 26, 318, 528, 1083
SatI GCNGC 2 cut(s) 345, 750
Sau3AI GATC 3 cut(s) 397, 787, 856
Sau96I GGNCC 3 cut(s) 251, 720, 751
ScaI AGTACT 2 cut(s) 479, 838
SchI GAGTC 1 cut(s) 968
ScrFI CCNGG 1 cut(s) 195
SfaNI GCATC 3 cut(s) 344, 802, 1004
SfoI GGCGCC 1 cut(s) 343
SfuI TTCGAA 2 cut(s) 451, 707
SinI GGWCC 2 cut(s) 251, 720
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 7 cut(s) 183, 257, 328, 532, 802, 818, 1048
SsiI CCGC 3 cut(s) 345, 723, 749
SspDI GGCGCC 1 cut(s) 341
SspMI CTAG 2 cut(s) 615, 1073
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 4 cut(s) 238, 463, 746, 836
TaiI ACGT 3 cut(s) 831, 891, 966
TaqI TCGA 4 cut(s) 355, 451, 707, 998
TasI AATT 7 cut(s) 183, 257, 328, 532, 802, 818, 1048
TatI WGTACW 4 cut(s) 463, 477, 633, 836
TauI GCSGC 2 cut(s) 347, 752
TfiI GAWTC 5 cut(s) 113, 274, 352, 412, 873
Tru1I TTAA 4 cut(s) 26, 318, 528, 1083
Tru9I TTAA 4 cut(s) 26, 318, 528, 1083
TscAI CASTG 3 cut(s) 21, 751, 1015
TseFI GTSAC 2 cut(s) 383, 1067
Tsp45I GTSAC 2 cut(s) 383, 1067
TspDTI ATGAA 5 cut(s) 62, 91, 529, 750, 811
TspGWI ACGGA 2 cut(s) 195, 1058
TspRI CASTG 3 cut(s) 21, 751, 1015
VpaK11BI GGWCC 2 cut(s) 251, 720
XapI RAATTY 2 cut(s) 257, 818
XceI RCATGY 1 cut(s) 729
XmnI GAANNNNTTC 2 cut(s) 217, 847
XspI CTAG 2 cut(s) 615, 1073
ZrmI AGTACT 2 cut(s) 479, 838
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.