Rh5BG092000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007491
Physical Location & Seq
Reverse (-)
8172006 .. 8177250
5245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG092000.1

Sequence Viewer

Length: 1089 bp
ATGACAAAAGATACTCTGAAATGGTTAATGGTGATTGCTGTTTTGGTGGTTTCAATATCACAATCTTGTGTTCATGGAGCGCCACAAGTACCTTGCTTCTTCATTTTCGGTGATTCATTGGCTGATAACGGCAATAACAACCTCCTCACGACTTTGGCCAAAGTGAACTACGCGCCGTACGGAATTGATTATCCTGGTGGAGTACGAACAGGAAGATTTTCCAATGGCAAAACTACTGTTGATATACTGGCTGAATTTCTGGGTTTTGAGACTCCCATCCCACCATTTGCAACTACCAAAGGACAGGACATTGTTAAAGGTCTCAATTATGCATCTGGCGCCGCAGGGATTCGAGCAGAAACTGGCTCCAATATGGGTGGTCACATCAGCTTAGATCAGCAGTTACTGAATCACCAAATCACAATCTTACGCATTGCTTTCATACTTCGAACAAATACACTACAAACCTTGCAGTACTTGAACAAGTGCCTATATTCAGTCGGAATGGGTAGCAATGATTACTTTAACAATTACTTCATGCCTCAGTTTTATCCCACTAGCAAGAGATACACCCCTGAACAATATGCTTCAGTTCTTATTGACCAATATACTAGCCAAATACTGACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACTAATAGGTTGCACTCCTAACGCGATTTTGAAAACGAAGGGGACCGCATGTGTAGATGAATGGAACAGTGCGGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTCAACACCAATTTGACTGATGCAAAATTCATCTATGTCAACAGTACTGGAATGAGTTCAGGGGATCCTACATCTGTTGGATTCAAGGTTTTGAACGTTGGGTGTTGTCCGGTGAATAATGTTGGACAATGTGTTCGTTCCGGAACTCCATGCCAGAATAGGAGTGAGTATGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACCAAATCACTGCATTCAGAACATACAAAAGTGTTGACCCAACGGATAATTATCCTATGGATGTCAGTCACCTGGTTCAGCTTAAACCATAA

Protein Analysis

362

Amino Acids

39.72

Weight (kDa)

8.28

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 337 8.7e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccII CGCG 2 cut(s) 173, 698
AccIII TCCGGA 1 cut(s) 929
AciI CCGC 3 cut(s) 342, 720, 746
AclI AACGTT 1 cut(s) 885
AclWI GGATC 2 cut(s) 848, 861
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 254, 815
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 7 cut(s) 90, 179, 204, 476, 632, 774, 835
AfiI CCNNNNNNNGG 1 cut(s) 988
AgsI TTSAA 6 cut(s) 54, 481, 706, 760, 874, 883
AjnI CCWGG 2 cut(s) 193, 1068
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 5 cut(s) 390, 754, 769, 790, 1078
AluI AGCT 5 cut(s) 390, 754, 769, 790, 1078
Alw26I GTCTC 2 cut(s) 263, 326
AlwI GGATC 2 cut(s) 848, 861
AlwNI CAGNNNCTG 2 cut(s) 362, 406
Aor13HI TCCGGA 1 cut(s) 929
AoxI GGCC 2 cut(s) 156, 747
ApoI RAATTY 2 cut(s) 254, 815
ArsI GACNNNNNNTTYG 2 cut(s) 906, 938
Asp700I GAANNNNTTC 2 cut(s) 217, 844
AspLEI GCGC 3 cut(s) 82, 175, 341
AspS9I GGNCC 2 cut(s) 717, 748
AsuHPI GGTGA 5 cut(s) 43, 122, 404, 913, 1058
AsuII TTCGAA 1 cut(s) 448
AvaII GGWCC 1 cut(s) 717
BalI TGGCCA 1 cut(s) 158
BamHI GGATCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 338
BccI CCATC 1 cut(s) 284
BceAI ACGGC 2 cut(s) 145, 160
BciT130I CCWGG 2 cut(s) 195, 1070
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 2 cut(s) 263, 326
BfaI CTAG 2 cut(s) 558, 612
BfoI RGCGCY 2 cut(s) 83, 342
BisI GCNGC 2 cut(s) 342, 747
BlsI GCNGC 2 cut(s) 343, 748
BmcAI AGTACT 2 cut(s) 476, 835
Bme1390I CCNGG 2 cut(s) 195, 1070
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 2 cut(s) 717, 748
BmiI GGNNCC 4 cut(s) 340, 367, 718, 855
BmrFI CCNGG 2 cut(s) 195, 1070
BmrI ACTGGG 1 cut(s) 680
BmsI GCATC 3 cut(s) 341, 799, 1001
BmuI ACTGGG 1 cut(s) 680
Bpu14I TTCGAA 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 660
BsaWI WCCGGW 2 cut(s) 898, 929
Bsc4I CCNNNNNNNGG 1 cut(s) 988
Bse1I ACTGG 4 cut(s) 252, 367, 675, 841
Bse3DI GCAATG 2 cut(s) 432, 520
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 929
BseBI CCWGG 2 cut(s) 195, 1070
BseDI CCNNGG 1 cut(s) 660
BseGI GGATG 2 cut(s) 276, 1063
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 1 cut(s) 988
BseMI GCAATG 2 cut(s) 432, 520
BseMII CTCAG 2 cut(s) 557, 978
BseNI ACTGG 4 cut(s) 252, 367, 675, 841
BseRI GAGGAG 1 cut(s) 134
BseYI CCCAGC 1 cut(s) 750
Bsh1236I CGCG 2 cut(s) 173, 698
BshFI GGCC 2 cut(s) 158, 749
BshNI GGYRCC 1 cut(s) 338
BsiSI CCGG 2 cut(s) 899, 930
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 3 cut(s) 687, 730, 983
BslI CCNNNNNNNGG 1 cut(s) 988
BsmAI GTCTC 2 cut(s) 263, 326
BsmFI GGGAC 3 cut(s) 687, 730, 983
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 2 cut(s) 158, 749
Bso31I GGTCTC 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 929
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 3 cut(s) 394, 784, 853
BspACI CCGC 3 cut(s) 342, 720, 746
BspANI GGCC 2 cut(s) 158, 749
BspCNI CTCAG 2 cut(s) 556, 979
BspEI TCCGGA 1 cut(s) 929
BspFNI CGCG 2 cut(s) 173, 698
BspLI GGNNCC 4 cut(s) 340, 367, 718, 855
BspPI GGATC 2 cut(s) 848, 861
BspT104I TTCGAA 1 cut(s) 448
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 432, 520
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 4 cut(s) 252, 367, 675, 841
BssECI CCNNGG 1 cut(s) 660
BssMI GATC 3 cut(s) 394, 784, 853
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 2 cut(s) 195, 1070
Bst4CI ACNGT 3 cut(s) 238, 743, 833
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 630
BstBI TTCGAA 1 cut(s) 448
BstDEI CTNAG 3 cut(s) 391, 543, 987
BstF5I GGATG 2 cut(s) 276, 1063
BstFNI CGCG 2 cut(s) 173, 698
BstH2I RGCGCY 2 cut(s) 83, 342
BstHHI GCGC 3 cut(s) 82, 175, 341
BstKTI GATC 3 cut(s) 397, 787, 856
BstMAI GTCTC 2 cut(s) 263, 326
BstMBI GATC 3 cut(s) 394, 784, 853
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 2 cut(s) 195, 1070
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 2 cut(s) 193, 1068
BstUI CGCG 2 cut(s) 173, 698
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuRI GGCC 2 cut(s) 158, 749
BtsCI GGATG 2 cut(s) 276, 1063
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 2 cut(s) 748, 1005
CaiI CAGNNNCTG 2 cut(s) 362, 406
CfoI GCGC 3 cut(s) 82, 175, 341
Cfr13I GGNCC 2 cut(s) 717, 748
CsiI ACCWGGT 1 cut(s) 1068
Csp6I GTAC 7 cut(s) 89, 178, 203, 475, 631, 773, 834
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 4 cut(s) 74, 538, 723, 939
CviQI GTAC 7 cut(s) 89, 178, 203, 475, 631, 773, 834
DdeI CTNAG 3 cut(s) 391, 543, 987
DinI GGCGCC 1 cut(s) 340
DpnI GATC 3 cut(s) 396, 786, 855
DpnII GATC 3 cut(s) 394, 784, 853
EaeI YGGCCR 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 717
Eco57I CTGAAG 1 cut(s) 573
EcoRII CCWGG 2 cut(s) 193, 1068
EcoT14I CCWWGG 1 cut(s) 660
EcoT22I ATGCAT 1 cut(s) 334
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 4 cut(s) 77, 541, 726, 942
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 3 cut(s) 687, 730, 983
FatI CATG 4 cut(s) 73, 537, 722, 938
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 2 cut(s) 342, 747
FokI GGATG 2 cut(s) 263, 1070
Fsp4HI GCNGC 2 cut(s) 342, 747
FspBI CTAG 2 cut(s) 558, 612
GlaI GCGC 3 cut(s) 81, 174, 340
GluI GCNGC 2 cut(s) 342, 747
GsaI CCCAGC 1 cut(s) 754
HaeII RGCGCY 2 cut(s) 83, 342
HaeIII GGCC 2 cut(s) 158, 749
HapII CCGG 2 cut(s) 899, 930
HhaI GCGC 3 cut(s) 82, 175, 341
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 4 cut(s) 77, 541, 726, 942
Hin6I GCGC 3 cut(s) 80, 173, 339
HinP1I GCGC 3 cut(s) 80, 173, 339
HincII GTYRAC 2 cut(s) 829, 1033
HindII GTYRAC 2 cut(s) 829, 1033
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 6 cut(s) 113, 271, 349, 409, 870, 971
HpaII CCGG 2 cut(s) 899, 930
HphI GGTGA 5 cut(s) 43, 122, 404, 913, 1058
Hpy166II GTNNAC 3 cut(s) 166, 829, 1033
Hpy188I TCNGA 3 cut(s) 18, 503, 1016
Hpy188III TCNNGA 2 cut(s) 148, 930
Hpy8I GTNNAC 3 cut(s) 166, 829, 1033
HpyAV CCTTC 2 cut(s) 646, 706
HpyCH4III ACNGT 3 cut(s) 238, 743, 833
HpyCH4IV ACGT 1 cut(s) 885
HpyCH4V TGCA 6 cut(s) 290, 332, 472, 687, 812, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 391, 543, 987
HpySE526I ACGT 1 cut(s) 885
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 4 cut(s) 77, 541, 726, 942
HspAI GCGC 3 cut(s) 80, 173, 339
KasI GGCGCC 1 cut(s) 338
Kpn2I TCCGGA 1 cut(s) 929
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 3 cut(s) 394, 784, 853
LmnI GCTCC 3 cut(s) 77, 371, 644
LweI GCATC 3 cut(s) 341, 799, 1001
MabI ACCWGGT 1 cut(s) 1068
MaeI CTAG 2 cut(s) 558, 612
MaeII ACGT 1 cut(s) 885
MaeIII GTNAC 3 cut(s) 380, 402, 1064
MalI GATC 3 cut(s) 396, 786, 855
MboI GATC 3 cut(s) 394, 784, 853
MboII GAAGA 2 cut(s) 91, 225
MflI RGATCY 1 cut(s) 853
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 7 cut(s) 183, 254, 325, 529, 799, 815, 1045
MluNI TGGCCA 1 cut(s) 158
Mly113I GGCGCC 1 cut(s) 339
MlyI GAGTC 2 cut(s) 265, 965
MmeI TCCRAC 4 cut(s) 481, 646, 847, 892
MnlI CCTC 5 cut(s) 152, 155, 552, 786, 982
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 929
MroXI GAANNNNTTC 2 cut(s) 217, 844
MscI TGGCCA 1 cut(s) 158
MseI TTAA 4 cut(s) 26, 315, 525, 1080
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 2 cut(s) 899, 930
MspR9I CCNGG 2 cut(s) 195, 1070
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 2 cut(s) 195, 1070
MvnI CGCG 2 cut(s) 173, 698
MwoI GCNNNNNNNGC 1 cut(s) 338
NarI GGCGCC 1 cut(s) 339
NdeII GATC 3 cut(s) 394, 784, 853
NlaIII CATG 4 cut(s) 77, 541, 726, 942
NlaIV GGNNCC 4 cut(s) 340, 367, 718, 855
NmuCI GTSAC 2 cut(s) 380, 1064
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 726
NspV TTCGAA 1 cut(s) 448
PctI GAATGC 1 cut(s) 1010
PdmI GAANNNNTTC 2 cut(s) 217, 844
PfeI GAWTC 4 cut(s) 113, 349, 409, 870
Pfl23II CGTACG 1 cut(s) 177
PkrI GCNGC 2 cut(s) 343, 748
PleI GAGTC 2 cut(s) 265, 965
PluTI GGCGCC 1 cut(s) 342
PpsI GAGTC 2 cut(s) 265, 965
Psp1406I AACGTT 1 cut(s) 885
Psp6I CCWGG 2 cut(s) 193, 1068
PspFI CCCAGC 1 cut(s) 750
PspGI CCWGG 2 cut(s) 193, 1068
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 4 cut(s) 340, 367, 718, 855
PspPI GGNCC 2 cut(s) 717, 748
PstNI CAGNNNCTG 2 cut(s) 362, 406
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 7 cut(s) 90, 179, 204, 476, 632, 774, 835
RsaNI GTAC 7 cut(s) 89, 178, 203, 475, 631, 773, 834
SaqAI TTAA 4 cut(s) 26, 315, 525, 1080
SatI GCNGC 2 cut(s) 342, 747
Sau3AI GATC 3 cut(s) 394, 784, 853
Sau96I GGNCC 2 cut(s) 717, 748
ScaI AGTACT 2 cut(s) 476, 835
SchI GAGTC 2 cut(s) 265, 965
ScrFI CCNGG 2 cut(s) 195, 1070
SexAI ACCWGGT 1 cut(s) 1068
SfaNI GCATC 3 cut(s) 341, 799, 1001
SfoI GGCGCC 1 cut(s) 340
SfuI TTCGAA 1 cut(s) 448
SinI GGWCC 1 cut(s) 717
Sse9I AATT 7 cut(s) 183, 254, 325, 529, 799, 815, 1045
SsiI CCGC 3 cut(s) 342, 720, 746
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 2 cut(s) 558, 612
StyD4I CCNGG 2 cut(s) 193, 1068
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 238, 743, 833
TaiI ACGT 1 cut(s) 888
TaqI TCGA 3 cut(s) 352, 448, 995
TasI AATT 7 cut(s) 183, 254, 325, 529, 799, 815, 1045
TatI WGTACW 3 cut(s) 474, 630, 833
TauI GCSGC 2 cut(s) 344, 749
TfiI GAWTC 4 cut(s) 113, 349, 409, 870
Tru1I TTAA 4 cut(s) 26, 315, 525, 1080
Tru9I TTAA 4 cut(s) 26, 315, 525, 1080
TscAI CASTG 2 cut(s) 748, 1012
TseFI GTSAC 2 cut(s) 380, 1064
Tsp45I GTSAC 2 cut(s) 380, 1064
TspDTI ATGAA 7 cut(s) 62, 91, 105, 430, 526, 747, 808
TspGWI ACGGA 2 cut(s) 195, 1055
TspRI CASTG 2 cut(s) 748, 1012
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 2 cut(s) 254, 815
XceI RCATGY 1 cut(s) 726
XmnI GAANNNNTTC 2 cut(s) 217, 844
XspI CTAG 2 cut(s) 558, 612
ZrmI AGTACT 2 cut(s) 476, 835
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.