Rh5DG091300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007491
Physical Location & Seq
Reverse (-)
8264298 .. 8277974
13677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG091300.1

Sequence Viewer

Length: 717 bp
ATGGGTGATCACATCAGCTTTGACCGGCAGTTACTGAATCACCAAATCACAACCTTGCGCATTGCTACAATACTTCGAGCAAATACACTACAAACCTTGCAGTACTTGAACAAGTGCCTATACACAGTGGGAATGGGTAACAATGATTACATTAACAATTACTTCATGCCTCAGTTTTATCCCACCAGCAAGAGATACACCCCTGAACAATATGCTTCAGTTCTTATTGACCAATATACTAGCCAAATACTGACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACTCATAGGTTGCACTCCTAATGCAATTTCGAAAACGAAGGGGATCACATGTGTAGATGAATTGAACAGTGCGGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTCAATACCAATTTGACTGATGCAAAATTCATCTATGTCAACAGTACTGGAATGAGTTCTGGGGATCCTACATCTGTTGGATTCAAGGTTTTGAATGTTGGGTGTTGTCCGGTGAATAGTGTTGGCCAATGTATTCGTTCCGGAACTCCATGCCAGAATAGGAGTGAGTATGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACCGAATTACTGCATTCAGAACATACGAAAGTGTTGACCCCACGGATAATTATCCTATGGATGTCAGTCACTTAGTTCAGCTTAAACTATAA

Protein Analysis

238

Amino Acids

26.64

Weight (kDa)

8.11

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 213 1.5e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 557
AciI CCGC 1 cut(s) 374
AclWI GGATC 3 cut(s) 353, 476, 489
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 4 cut(s) 104, 260, 402, 463
AfiI CCNNNNNNNGG 1 cut(s) 616
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 5 cut(s) 109, 367, 388, 502, 511
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 5 cut(s) 18, 382, 397, 418, 706
AluI AGCT 5 cut(s) 18, 382, 397, 418, 706
AlwI GGATC 3 cut(s) 353, 476, 489
AlwNI CAGNNNCTG 1 cut(s) 34
Aor13HI TCCGGA 1 cut(s) 557
AoxI GGCC 2 cut(s) 375, 541
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 3 cut(s) 17, 32, 541
AsuII TTCGAA 1 cut(s) 332
BalI TGGCCA 1 cut(s) 543
BamHI GGATCC 1 cut(s) 481
BclI TGATCA 2 cut(s) 7, 412
BfaI CTAG 1 cut(s) 240
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
BmcAI AGTACT 2 cut(s) 104, 463
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 483
BmrI ACTGGG 1 cut(s) 308
BmsI GCATC 2 cut(s) 427, 629
BmuI ACTGGG 1 cut(s) 308
Bpu14I TTCGAA 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 2 cut(s) 288, 666
BsaWI WCCGGW 2 cut(s) 526, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 303, 469
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 675
BseAI TCCGGA 1 cut(s) 557
BseDI CCNNGG 2 cut(s) 288, 666
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 2 cut(s) 185, 606
BseNI ACTGG 2 cut(s) 303, 469
BseYI CCCAGC 1 cut(s) 378
BshFI GGCC 2 cut(s) 377, 543
BsiSI CCGG 3 cut(s) 25, 527, 558
BslFI GGGAC 2 cut(s) 315, 611
BslI CCNNNNNNNGG 1 cut(s) 616
BsmFI GGGAC 2 cut(s) 315, 611
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 2 cut(s) 377, 543
Bsp119I TTCGAA 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 557
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 4 cut(s) 7, 345, 412, 481
BspACI CCGC 1 cut(s) 374
BspANI GGCC 2 cut(s) 377, 543
BspCNI CTCAG 2 cut(s) 184, 607
BspEI TCCGGA 1 cut(s) 557
BspLI GGNNCC 1 cut(s) 483
BspPI GGATC 3 cut(s) 353, 476, 489
BspT104I TTCGAA 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 303, 469
BssAI RCCGGY 1 cut(s) 24
BssECI CCNNGG 2 cut(s) 288, 666
BssMI GATC 4 cut(s) 7, 345, 412, 481
BssT1I CCWWGG 1 cut(s) 288
Bst4CI ACNGT 3 cut(s) 127, 371, 461
BstAUI TGTACA 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 332
BstDEI CTNAG 3 cut(s) 171, 615, 697
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 1 cut(s) 691
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 4 cut(s) 10, 348, 415, 484
BstMBI GATC 4 cut(s) 7, 345, 412, 481
BstNSI RCATGY 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BsuRI GGCC 2 cut(s) 377, 543
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 691
BtsIMutI CAGTG 2 cut(s) 132, 376
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 4 cut(s) 103, 259, 401, 462
CviAII CATG 3 cut(s) 166, 351, 567
CviJI RGCY 8 cut(s) 18, 243, 377, 382, 397, 418, 543, 706
CviKI_1 RGCY 8 cut(s) 18, 243, 377, 382, 397, 418, 543, 706
CviQI GTAC 4 cut(s) 103, 259, 401, 462
DdeI CTNAG 3 cut(s) 171, 615, 697
DpnI GATC 4 cut(s) 9, 347, 414, 483
DpnII GATC 4 cut(s) 7, 345, 412, 481
EaeI YGGCCR 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 3 cut(s) 169, 354, 570
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 2 cut(s) 315, 611
FatI CATG 3 cut(s) 165, 350, 566
FbaI TGATCA 2 cut(s) 7, 412
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 1 cut(s) 698
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 1 cut(s) 240
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 375
GsaI CCCAGC 1 cut(s) 382
HaeIII GGCC 2 cut(s) 377, 543
HapII CCGG 3 cut(s) 25, 527, 558
HhaI GCGC 1 cut(s) 60
Hin1II CATG 3 cut(s) 169, 354, 570
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 2 cut(s) 457, 661
HindII GTYRAC 2 cut(s) 457, 661
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 4 cut(s) 37, 303, 498, 599
HpaII CCGG 3 cut(s) 25, 527, 558
HphI GGTGA 3 cut(s) 17, 32, 541
Hpy166II GTNNAC 2 cut(s) 457, 661
Hpy188I TCNGA 1 cut(s) 644
Hpy188III TCNNGA 1 cut(s) 558
Hpy8I GTNNAC 2 cut(s) 457, 661
HpyAV CCTTC 2 cut(s) 274, 334
HpyCH4III ACNGT 3 cut(s) 127, 371, 461
HpyCH4V TGCA 5 cut(s) 100, 315, 326, 440, 638
HpyF3I CTNAG 3 cut(s) 171, 615, 697
Hsp92II CATG 3 cut(s) 169, 354, 570
HspAI GCGC 1 cut(s) 58
Kpn2I TCCGGA 1 cut(s) 557
Ksp22I TGATCA 2 cut(s) 7, 412
Kzo9I GATC 4 cut(s) 7, 345, 412, 481
LmnI GCTCC 1 cut(s) 272
LweI GCATC 2 cut(s) 427, 629
MaeI CTAG 1 cut(s) 240
MaeIII GTNAC 3 cut(s) 30, 137, 692
MalI GATC 4 cut(s) 9, 347, 414, 483
MboI GATC 4 cut(s) 7, 345, 412, 481
MflI RGATCY 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 7 cut(s) 157, 327, 362, 427, 443, 630, 673
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 2 cut(s) 297, 593
MmeI TCCRAC 2 cut(s) 274, 475
MnlI CCTC 3 cut(s) 180, 414, 610
Mox20I TGGCCA 1 cut(s) 543
MroI TCCGGA 1 cut(s) 557
MroXI GAANNNNTTC 1 cut(s) 472
MscI TGGCCA 1 cut(s) 543
MseI TTAA 2 cut(s) 153, 708
Msp20I TGGCCA 1 cut(s) 543
MspI CCGG 3 cut(s) 25, 527, 558
Mva1269I GAATGC 1 cut(s) 638
NdeII GATC 4 cut(s) 7, 345, 412, 481
NlaIII CATG 3 cut(s) 169, 354, 570
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 1 cut(s) 692
NsbI TGCGCA 1 cut(s) 59
NspI RCATGY 1 cut(s) 354
NspV TTCGAA 1 cut(s) 332
PciI ACATGT 1 cut(s) 350
PctI GAATGC 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 37, 498
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 2 cut(s) 297, 593
PpsI GAGTC 2 cut(s) 297, 593
PscI ACATGT 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 481
RsaI GTAC 4 cut(s) 104, 260, 402, 463
RsaNI GTAC 4 cut(s) 103, 259, 401, 462
SaqAI TTAA 2 cut(s) 153, 708
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 4 cut(s) 7, 345, 412, 481
Sau96I GGNCC 1 cut(s) 376
ScaI AGTACT 2 cut(s) 104, 463
SchI GAGTC 2 cut(s) 297, 593
SfaNI GCATC 2 cut(s) 427, 629
SfuI TTCGAA 1 cut(s) 332
Sse9I AATT 7 cut(s) 157, 327, 362, 427, 443, 630, 673
SsiI CCGC 1 cut(s) 374
SspMI CTAG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 127, 371, 461
TaqI TCGA 3 cut(s) 76, 332, 623
TasI AATT 7 cut(s) 157, 327, 362, 427, 443, 630, 673
TatI WGTACW 3 cut(s) 102, 258, 461
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 37, 498
Tru1I TTAA 2 cut(s) 153, 708
Tru9I TTAA 2 cut(s) 153, 708
TscAI CASTG 2 cut(s) 132, 376
TseFI GTSAC 1 cut(s) 692
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 3 cut(s) 154, 375, 436
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 2 cut(s) 132, 376
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 1 cut(s) 240
ZrmI AGTACT 2 cut(s) 104, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.