Rroxscaffold_1G00064220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007491
Physical Location & Seq
Forward (+)
86050057 .. 86051091
1035 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00064220.1

Sequence Viewer

Length: 585 bp
ATGGGTAGCAATGATTACTTTAATAATTATTTCATGCCTCAGTTTTATCCCACTAGCAAGAGATACACCCCTGAACAATATGCTTCAGTTCTTATTGACCAATATACTAGCCAAATACTGACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACTAATAGGTTGCACTCCTAACGCGATTTCGAAAACGAAGGGGACCGGATGTGTAGATGAATGGAACAGTGCGGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTCGACACCAATTTGACTGATGCAAAATTCATCTATGTCAACAGTACTGGAATGAGTTCAGGGGATCCTACATCTGTTGGATTCAAGGTTTTGAACGTTGGGTGTTGTCCGGTGAATAATGTTGGACAATGTGTTCGTTCCGGAATTCCATGCCAAAATAGGAGTGAGTATGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACAAAATCACTGCATTCAGAACATACAAAAGTGTTGACCCAACGGATAATTATCCTATGGATGTCAGTCACCTAGTTCAGCTTATACCATAA

Protein Analysis

194

Amino Acids

21.56

Weight (kDa)

6.69

Isoelectric Point (pI)

23.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 1 - 169 7.5e-18 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 1 cut(s) 242
AclI AACGTT 1 cut(s) 381
AclWI GGATC 2 cut(s) 344, 357
AcsI RAATTY 2 cut(s) 311, 429
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 3 cut(s) 128, 270, 331
AfiI CCNNNNNNNGG 1 cut(s) 484
AgsI TTSAA 3 cut(s) 256, 370, 379
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AloI GAACNNNNNNTCC 2 cut(s) 402, 434
AluBI AGCT 4 cut(s) 250, 265, 286, 574
AluI AGCT 4 cut(s) 250, 265, 286, 574
AlwI GGATC 2 cut(s) 344, 357
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 1 cut(s) 243
ApoI RAATTY 2 cut(s) 311, 429
ArsI GACNNNNNNTTYG 2 cut(s) 402, 434
Asp700I GAANNNNTTC 1 cut(s) 340
AspS9I GGNCC 2 cut(s) 213, 244
AsuHPI GGTGA 2 cut(s) 409, 554
AsuII TTCGAA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 213
BamHI GGATCC 1 cut(s) 349
BclI TGATCA 1 cut(s) 280
BfaI CTAG 3 cut(s) 54, 108, 566
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
BmcAI AGTACT 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 213, 244
BmiI GGNNCC 2 cut(s) 214, 351
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 295, 497
BmuI ACTGGG 1 cut(s) 176
Bpu14I TTCGAA 1 cut(s) 200
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 1 cut(s) 156
BsaWI WCCGGW 3 cut(s) 215, 394, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse1I ACTGG 2 cut(s) 171, 337
Bse3DI GCAATG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 2 cut(s) 224, 559
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 53, 474
BseNI ACTGG 2 cut(s) 171, 337
BseYI CCCAGC 1 cut(s) 246
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 1 cut(s) 245
BsiSI CCGG 3 cut(s) 216, 395, 426
BslFI GGGAC 3 cut(s) 183, 226, 479
BslI CCNNNNNNNGG 1 cut(s) 484
BsmFI GGGAC 3 cut(s) 183, 226, 479
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 2 cut(s) 280, 349
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 2 cut(s) 52, 475
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 214, 351
BspPI GGATC 2 cut(s) 344, 357
BspT104I TTCGAA 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 2 cut(s) 171, 337
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 2 cut(s) 280, 349
BssT1I CCWWGG 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 239, 329
BstAUI TGTACA 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 200
BstDEI CTNAG 2 cut(s) 39, 483
BstF5I GGATG 2 cut(s) 224, 559
BstFNI CGCG 1 cut(s) 194
BstKTI GATC 2 cut(s) 283, 352
BstMBI GATC 2 cut(s) 280, 349
BstUI CGCG 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 1 cut(s) 245
BtsCI GGATG 2 cut(s) 224, 559
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 2 cut(s) 244, 501
Cfr13I GGNCC 2 cut(s) 213, 244
Csp6I GTAC 3 cut(s) 127, 269, 330
CviAII CATG 2 cut(s) 34, 435
CviJI RGCY 6 cut(s) 111, 245, 250, 265, 286, 574
CviKI_1 RGCY 6 cut(s) 111, 245, 250, 265, 286, 574
CviQI GTAC 3 cut(s) 127, 269, 330
DdeI CTNAG 2 cut(s) 39, 483
DpnI GATC 2 cut(s) 282, 351
DpnII GATC 2 cut(s) 280, 349
Eco130I CCWWGG 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 69
EcoRI GAATTC 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 2 cut(s) 37, 438
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 3 cut(s) 183, 226, 479
FatI CATG 2 cut(s) 33, 434
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 2 cut(s) 231, 566
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 3 cut(s) 54, 108, 566
GluI GCNGC 1 cut(s) 243
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 1 cut(s) 245
HapII CCGG 3 cut(s) 216, 395, 426
Hin1II CATG 2 cut(s) 37, 438
HincII GTYRAC 2 cut(s) 325, 529
HindII GTYRAC 2 cut(s) 325, 529
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 366, 467
HpaII CCGG 3 cut(s) 216, 395, 426
HphI GGTGA 2 cut(s) 409, 554
Hpy166II GTNNAC 2 cut(s) 325, 529
Hpy188I TCNGA 1 cut(s) 512
Hpy188III TCNNGA 1 cut(s) 426
Hpy8I GTNNAC 2 cut(s) 325, 529
HpyAV CCTTC 2 cut(s) 142, 202
HpyCH4III ACNGT 2 cut(s) 239, 329
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 3 cut(s) 183, 308, 506
HpyF3I CTNAG 2 cut(s) 39, 483
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 2 cut(s) 37, 438
Kpn2I TCCGGA 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 2 cut(s) 280, 349
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 9 cut(s) 84, 152, 229, 260, 318, 330, 408, 439, 448
LweI GCATC 2 cut(s) 295, 497
MaeI CTAG 3 cut(s) 54, 108, 566
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 560
MalI GATC 2 cut(s) 282, 351
MboI GATC 2 cut(s) 280, 349
MflI RGATCY 1 cut(s) 349
MluCI AATT 5 cut(s) 25, 295, 311, 429, 541
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 3 cut(s) 142, 343, 388
MnlI CCTC 3 cut(s) 48, 282, 478
MroI TCCGGA 1 cut(s) 425
MroXI GAANNNNTTC 1 cut(s) 340
MseI TTAA 1 cut(s) 21
MspI CCGG 3 cut(s) 216, 395, 426
Mva1269I GAATGC 1 cut(s) 506
MvnI CGCG 1 cut(s) 194
NdeII GATC 2 cut(s) 280, 349
NlaIII CATG 2 cut(s) 37, 438
NlaIV GGNNCC 2 cut(s) 214, 351
NmuCI GTSAC 1 cut(s) 560
NspV TTCGAA 1 cut(s) 200
PctI GAATGC 1 cut(s) 506
PdmI GAANNNNTTC 1 cut(s) 340
PfeI GAWTC 1 cut(s) 366
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
Psp1406I AACGTT 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 214, 351
PspPI GGNCC 2 cut(s) 213, 244
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 3 cut(s) 128, 270, 331
RsaNI GTAC 3 cut(s) 127, 269, 330
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 280, 349
Sau96I GGNCC 2 cut(s) 213, 244
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 1 cut(s) 461
SfaNI GCATC 2 cut(s) 295, 497
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 1 cut(s) 213
Sse9I AATT 5 cut(s) 25, 295, 311, 429, 541
SsiI CCGC 1 cut(s) 242
SspMI CTAG 3 cut(s) 54, 108, 566
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 239, 329
TaiI ACGT 1 cut(s) 384
TaqI TCGA 3 cut(s) 200, 288, 491
TasI AATT 5 cut(s) 25, 295, 311, 429, 541
TatI WGTACW 2 cut(s) 126, 329
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 244, 508
TseFI GTSAC 1 cut(s) 560
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 3 cut(s) 22, 243, 304
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 2 cut(s) 244, 508
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 2 cut(s) 311, 429
XmnI GAANNNNTTC 1 cut(s) 340
XspI CTAG 3 cut(s) 54, 108, 566
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.