Rh5AG095700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007491
Physical Location & Seq
Reverse (-)
8756880 .. 8762732
5853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG095700.1

Sequence Viewer

Length: 849 bp
ATGGAGCTCTCTGAATTTCTGGGTTTTGAGAATCCCATCCCACCATTTGCAACTACCAACATACAGGACATTGTTAAAGGTCTTAATTATGCATCTGGCGCCGCAGGGATTCGAGCAGAAACTGGCTCCAATTTGGGTGATCACATCAGCTTAGACCGGCAGTTACTGAATCACCAAATCACAACCTTGCGCATTGCTTCAATACTTCGAGCAAATACACTACAAACCTTGCAGTACTTGAACAAGTGCCTATACATAGTGGGAATGGGTAACAATGATTACATTAACAATTACTTCATGCCTCAGTTTTATCCCACCAGCAAGAGATACACCCCTGAACAATATGCTTCGGTTCTTATTGACCAATATACTAGCCAAATACTGACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACTCATAGGTTGCACTCCTAATGCAATTTCGAAAACGAAGGGGATCACATGTGTAGATGAATTGAACAGTGCGGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTCAATACCAATTTGACTGATGCAAAATTCATCTATGTCAACAGTACTGGAATGAGTTCTGGGGATCCTACATCTGTTGGATTCAAGGTTTTGAATGTTGGGTGTTGTCCGGTGAATAGTGTTGGCCAATGTATTCGTTCCGGAACTCCATGCCAGAATAGGAGTGAGTATGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACCGAATTACTGCATTCAGAACATACGAAAGTGTTGACCCCACGGATAATTATCCTATGGATGTCAGTCACTTAGTTCAGCTTAAACTATAA

Protein Analysis

282

Amino Acids

31.21

Weight (kDa)

6.41

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 4 - 257 1.4e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 98
AccIII TCCGGA 1 cut(s) 689
AciI CCGC 2 cut(s) 102, 506
AclWI GGATC 3 cut(s) 485, 608, 621
AcoI YGGCCR 1 cut(s) 673
AcsI RAATTY 2 cut(s) 14, 575
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 4 cut(s) 236, 392, 534, 595
AfiI CCNNNNNNNGG 1 cut(s) 748
AflIII ACRYGT 1 cut(s) 482
AgsI TTSAA 6 cut(s) 201, 241, 499, 520, 634, 643
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AluBI AGCT 6 cut(s) 7, 150, 514, 529, 550, 838
AluI AGCT 6 cut(s) 7, 150, 514, 529, 550, 838
Alw21I GWGCWC 1 cut(s) 9
AlwI GGATC 3 cut(s) 485, 608, 621
AlwNI CAGNNNCTG 2 cut(s) 122, 166
Aor13HI TCCGGA 1 cut(s) 689
AoxI GGCC 2 cut(s) 507, 673
ApoI RAATTY 2 cut(s) 14, 575
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 101, 192
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 3 cut(s) 149, 164, 673
AsuII TTCGAA 1 cut(s) 464
BalI TGGCCA 1 cut(s) 675
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 1 cut(s) 44
BclI TGATCA 2 cut(s) 139, 544
BfaI CTAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 102
BisI GCNGC 2 cut(s) 102, 507
BlsI GCNGC 2 cut(s) 103, 508
BmcAI AGTACT 2 cut(s) 236, 595
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 3 cut(s) 100, 127, 615
BmrI ACTGGG 1 cut(s) 440
BmsI GCATC 3 cut(s) 101, 559, 761
BmuI ACTGGG 1 cut(s) 440
Bpu14I TTCGAA 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 420, 798
BsaWI WCCGGW 2 cut(s) 658, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 748
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 3 cut(s) 127, 435, 601
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 807
BseAI TCCGGA 1 cut(s) 689
BseDI CCNNGG 2 cut(s) 420, 798
BseGI GGATG 2 cut(s) 36, 823
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 1 cut(s) 748
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 317, 738
BseNI ACTGG 3 cut(s) 127, 435, 601
BseYI CCCAGC 1 cut(s) 510
BshFI GGCC 2 cut(s) 509, 675
BshNI GGYRCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 3 cut(s) 157, 659, 690
BslFI GGGAC 2 cut(s) 447, 743
BslI CCNNNNNNNGG 1 cut(s) 748
BsmFI GGGAC 2 cut(s) 447, 743
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 2 cut(s) 509, 675
Bsp119I TTCGAA 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 9
Bsp13I TCCGGA 1 cut(s) 689
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 4 cut(s) 139, 477, 544, 613
BspACI CCGC 2 cut(s) 102, 506
BspANI GGCC 2 cut(s) 509, 675
BspCNI CTCAG 2 cut(s) 316, 739
BspEI TCCGGA 1 cut(s) 689
BspLI GGNNCC 3 cut(s) 100, 127, 615
BspPI GGATC 3 cut(s) 485, 608, 621
BspT104I TTCGAA 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 3 cut(s) 127, 435, 601
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 420, 798
BssMI GATC 4 cut(s) 139, 477, 544, 613
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 503, 593
BstACI GRCGYC 1 cut(s) 99
BstAUI TGTACA 1 cut(s) 390
BstBI TTCGAA 1 cut(s) 464
BstDEI CTNAG 4 cut(s) 151, 303, 747, 829
BstDSI CCRYGG 1 cut(s) 798
BstF5I GGATG 2 cut(s) 36, 823
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 101, 192
BstKTI GATC 4 cut(s) 142, 480, 547, 616
BstMBI GATC 4 cut(s) 139, 477, 544, 613
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuRI GGCC 2 cut(s) 509, 675
BtgI CCRYGG 1 cut(s) 798
BtsCI GGATG 2 cut(s) 36, 823
BtsIMutI CAGTG 1 cut(s) 508
CaiI CAGNNNCTG 2 cut(s) 122, 166
CfoI GCGC 2 cut(s) 101, 192
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 508
Csp6I GTAC 4 cut(s) 235, 391, 533, 594
CviAII CATG 3 cut(s) 298, 483, 699
CviQI GTAC 4 cut(s) 235, 391, 533, 594
DdeI CTNAG 4 cut(s) 151, 303, 747, 829
DinI GGCGCC 1 cut(s) 100
DpnI GATC 4 cut(s) 141, 479, 546, 615
DpnII GATC 4 cut(s) 139, 477, 544, 613
EaeI YGGCCR 1 cut(s) 673
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 420
EcoT22I ATGCAT 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 9
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 3 cut(s) 301, 486, 702
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 2 cut(s) 447, 743
FatI CATG 3 cut(s) 297, 482, 698
FbaI TGATCA 2 cut(s) 139, 544
Fnu4HI GCNGC 2 cut(s) 102, 507
FokI GGATG 2 cut(s) 23, 830
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 102, 507
FspBI CTAG 1 cut(s) 372
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 2 cut(s) 100, 191
GluI GCNGC 2 cut(s) 102, 507
GsaI CCCAGC 1 cut(s) 514
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 509, 675
HapII CCGG 3 cut(s) 157, 659, 690
HhaI GCGC 2 cut(s) 101, 192
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 3 cut(s) 301, 486, 702
Hin6I GCGC 2 cut(s) 99, 190
HinP1I GCGC 2 cut(s) 99, 190
HincII GTYRAC 2 cut(s) 589, 793
HindII GTYRAC 2 cut(s) 589, 793
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 6 cut(s) 31, 109, 169, 435, 630, 731
HpaII CCGG 3 cut(s) 157, 659, 690
HphI GGTGA 3 cut(s) 149, 164, 673
Hpy166II GTNNAC 2 cut(s) 589, 793
Hpy188I TCNGA 2 cut(s) 13, 776
Hpy188III TCNNGA 1 cut(s) 690
Hpy8I GTNNAC 2 cut(s) 589, 793
HpyAV CCTTC 2 cut(s) 406, 466
HpyCH4III ACNGT 2 cut(s) 503, 593
HpyCH4V TGCA 7 cut(s) 50, 92, 232, 447, 458, 572, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 4 cut(s) 151, 303, 747, 829
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 3 cut(s) 301, 486, 702
HspAI GCGC 2 cut(s) 99, 190
KasI GGCGCC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 689
Ksp22I TGATCA 2 cut(s) 139, 544
Kzo9I GATC 4 cut(s) 139, 477, 544, 613
LmnI GCTCC 3 cut(s) 4, 131, 404
LweI GCATC 3 cut(s) 101, 559, 761
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 3 cut(s) 162, 269, 824
MalI GATC 4 cut(s) 141, 479, 546, 615
MboI GATC 4 cut(s) 139, 477, 544, 613
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 1 cut(s) 675
MluNI TGGCCA 1 cut(s) 675
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 2 cut(s) 429, 725
MmeI TCCRAC 2 cut(s) 406, 607
MnlI CCTC 3 cut(s) 312, 546, 742
Mox20I TGGCCA 1 cut(s) 675
Mph1103I ATGCAT 1 cut(s) 94
MroI TCCGGA 1 cut(s) 689
MroXI GAANNNNTTC 1 cut(s) 604
MscI TGGCCA 1 cut(s) 675
MseI TTAA 4 cut(s) 75, 84, 285, 840
Msp20I TGGCCA 1 cut(s) 675
MspI CCGG 3 cut(s) 157, 659, 690
Mva1269I GAATGC 1 cut(s) 770
MwoI GCNNNNNNNGC 1 cut(s) 98
NarI GGCGCC 1 cut(s) 99
NdeII GATC 4 cut(s) 139, 477, 544, 613
NlaIII CATG 3 cut(s) 301, 486, 702
NlaIV GGNNCC 3 cut(s) 100, 127, 615
NmuCI GTSAC 1 cut(s) 824
NsbI TGCGCA 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 1 cut(s) 486
NspV TTCGAA 1 cut(s) 464
PciI ACATGT 1 cut(s) 482
PctI GAATGC 1 cut(s) 770
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 4 cut(s) 31, 109, 169, 630
PkrI GCNGC 2 cut(s) 103, 508
PleI GAGTC 2 cut(s) 429, 725
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 2 cut(s) 429, 725
PscI ACATGT 1 cut(s) 482
Psp124BI GAGCTC 1 cut(s) 9
PspFI CCCAGC 1 cut(s) 510
PspN4I GGNNCC 3 cut(s) 100, 127, 615
PspPI GGNCC 1 cut(s) 508
PstNI CAGNNNCTG 2 cut(s) 122, 166
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 4 cut(s) 236, 392, 534, 595
RsaNI GTAC 4 cut(s) 235, 391, 533, 594
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 4 cut(s) 75, 84, 285, 840
SatI GCNGC 2 cut(s) 102, 507
Sau3AI GATC 4 cut(s) 139, 477, 544, 613
Sau96I GGNCC 1 cut(s) 508
ScaI AGTACT 2 cut(s) 236, 595
SchI GAGTC 2 cut(s) 429, 725
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 3 cut(s) 101, 559, 761
SfoI GGCGCC 1 cut(s) 100
SfuI TTCGAA 1 cut(s) 464
SsiI CCGC 2 cut(s) 102, 506
SspDI GGCGCC 1 cut(s) 98
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 9
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 2 cut(s) 503, 593
TaqI TCGA 4 cut(s) 112, 208, 464, 755
TatI WGTACW 3 cut(s) 234, 390, 593
TauI GCSGC 2 cut(s) 104, 509
TfiI GAWTC 4 cut(s) 31, 109, 169, 630
Tru1I TTAA 4 cut(s) 75, 84, 285, 840
Tru9I TTAA 4 cut(s) 75, 84, 285, 840
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 1 cut(s) 824
Tsp45I GTSAC 1 cut(s) 824
TspDTI ATGAA 3 cut(s) 286, 507, 568
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 1 cut(s) 508
XapI RAATTY 2 cut(s) 14, 575
XceI RCATGY 1 cut(s) 486
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 1 cut(s) 372
ZrmI AGTACT 2 cut(s) 236, 595
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.