Rorug05G0001500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007491
Physical Location & Seq
Reverse (-)
109978 .. 111999
2022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0001500.1

Sequence Viewer

Length: 2022 bp
ATGAAGAGACAGATTTTGTGTAGTCAATCCAAGGCCTTATTATTGTTGGACAAGTACAACAATGCTGCTGCTGCTCTTGGTATTCGGAAACTTCCATTATCGGCGCAGATGGGCAGCGCTGCCACTCCGGCTGACTTGACTACTGACTCAGACATTTCAGAAGGCGAAGACTGGTACGCCTCCCATGTCATCCAAGACAAGGACCTTCTCCGCAAGAGTCTCACAGATTCGGGAACAACAGGGCTCCATGTTCTGGACTTGGTTAATCGCGCTTCTTTGGAAGCTGACCGGACCCTGTACAATAAGCTGCTTAACAAATGTACCCAGACTGGCCAACTTAAACAGGCCAGAATTGTCCGTGCTCACATCCTTACTTCCAAGTTCAACGATGACCTTCCCATCCACAACACCATTCTCAACACGTACGTCAAGTGCGGCAGCTTGGACGACGCCCACAAGCTGTTCGATGAAATGCCTCACAGGGATTTGGTCACCTGGACAGCCTTGATCTCTGGCTATTCTCAACATGATCGACCTGAGGAAGCACTTGTTCTGCTTCCTCAAATGCTTCGCCATGGACTGCAACCCAACCAGTTCACGCTGTCTAGCTTGCTCAAGGCTGCTGGTGCTGGGCCTGACCCTGATGATAAGCACGGCAGGCAGCTTCATGCCCACTGCTTCAAGTGCGGTTTTCATTCCAATGTTTATGTGGGCACTTCTCTCGTGGACGTGTATGCTAGGTGTGGCCGTATGGACGAATCCCAGATCATCTTTGACGCTCTCGACACCAAGAATGAGGTGTCTTGGAATGCTTTGATCGCCGGGCATGCTAGGAAGGGTCAAGGAGAGCATGCTCTGAGGTTGTTCTGGAAGATGCTGAGGGGAGGATTCAAGCCCACTCATTTCACTTATTCTAGTGTGTTTACTGTTTGTGCCAGTGCAGGGTCTATGGAGCAAGGTAAGTGGGTTCACTCCCATATGATCAAATCGGGTGTTCACCTTGTTGCTTTTGTTGGGAATACTCTTCTCGACATGTATGCCAAGTCAGGCAGCATTGATGATGCTAAAAAGGTATTTGACCGGTTGGTTAGACGAGACATTGTTTCTTGGAATTCGATGCTTACTGGATACTCCCAGCATGGGCTTGGGAAGGAAACTGTGCAGCTGTTTGAAGAAATGCTTAGGATTGGAATTCAACCTAATGATATAACATTCCTCTGTGTTCTTACTGCCTGTAGCCATGCTCGCCTCTTGGATGAAGGACAGTATTATTTTCACTTGATGAGGAAAAGGTTCAAAATAGAACCACAGGTTTGGCATTACGTTACAATGGTTGATCTTCTTGGCCGAGCAGGTCTTCTTGATAGAGCTGACAAGTTCATAAGAGAAATGCCGATTGAACCCACTGCAGCTGTCTGGGGAGCCTTGCTAGGTGCTTGTAGGATGCACAACAACATCGACTTGGGTGCCTATGCTGCTGAGCGTGCTTTTGATCTTGATCCTTATGATTCAGGACCCTATATTATACTCGCTAATATGTACGCATCTGCTGGAAGATTGAATGATGCTGCAAAAGTGAGAAAGATGATGAAAGAGAGTGGAGTGAAAAAGGAACCTGCCTGTAGTTGGGTGGAGATTGAGAATGCAGTCCACATGTTTGTGGCAAACGATGATGCACACCCGCAAAGAGCAGAGATATATAGGATGTGGGAGATGATTAGCAAGAAGATAAAGGAGGTCGGATACGTTCCAGACACTAGCCATGTACTCTTCTTTGTGGACCAGCAGGAGAGGGAAGTCAAGTTGCAGTTTCACAGTGAGAAGCTTGCACTCGCATTTGCGCTTCTTAACACTTCTCCTGGATCCACCATCCGGATCAAGAAGAACATCAGGGTTTGTGGTGATTGTCATTCGGCAATCAAGTACGTCTCAAAGGTGGAAAGGCGAGAAATCATTGTGAGAGACACCAATCGGTTCCATCATTTCCGTGATGGCTCTTGTTCTTGTGGGGACTATTGGTAG

Protein Analysis

673

Amino Acids

75.83

Weight (kDa)

8.36

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 162 - 207 3e-11 PPR repeat family
PPR PF01535 164 - 194 4.3e-07 PPR repeat
PPR_2 PF13041 265 - 312 5.1e-13 PPR repeat family
PPR PF01535 267 - 297 1.2e-06 PPR repeat
PPR_2 PF13041 365 - 413 3.9e-12 PPR repeat family
PPR PF01535 368 - 398 1.4e-07 PPR repeat
E_motif PF20431 483 - 545 6.7e-20 E motif
DYW_deaminase PF14432 581 - 673 1.6e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1343, 1624
AccB1I GGYRCC 1 cut(s) 1466
AccB7I CCANNNNNTGG 1 cut(s) 253
AccII CGCG 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 1872
AciI CCGC 4 cut(s) 211, 435, 687, 1682
AclWI GGATC 4 cut(s) 1493, 1857, 1870, 1883
AcoI YGGCCR 3 cut(s) 331, 745, 1345
AcsI RAATTY 2 cut(s) 1111, 1191
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 8 cut(s) 56, 176, 299, 322, 425, 1541, 1767, 1925
AfeI AGCGCT 1 cut(s) 118
AfiI CCNNNNNNNGG 6 cut(s) 199, 253, 942, 1140, 1626, 1872
AflIII ACRYGT 4 cut(s) 420, 729, 1032, 1653
AgeI ACCGGT 1 cut(s) 1080
AgsI TTSAA 8 cut(s) 385, 682, 892, 1172, 1196, 1297, 1400, 1561
AjiI CACGTC 1 cut(s) 730
AjnI CCWGG 2 cut(s) 494, 1858
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 4 cut(s) 224, 1089, 1933, 1956
AlwI GGATC 4 cut(s) 1493, 1857, 1870, 1883
Aor13HI TCCGGA 1 cut(s) 1872
Aor51HI AGCGCT 1 cut(s) 118
AoxI GGCC 6 cut(s) 33, 331, 345, 632, 745, 1345
ApoI RAATTY 2 cut(s) 1111, 1191
AsiGI ACCGGT 1 cut(s) 1080
Asp700I GAANNNNTTC 1 cut(s) 1292
AspLEI GCGC 4 cut(s) 106, 119, 272, 1843
AspS9I GGNCC 5 cut(s) 202, 291, 632, 1514, 1780
AsuC2I CCSGG 1 cut(s) 823
AsuHPI GGTGA 3 cut(s) 484, 989, 1913
AvaII GGWCC 4 cut(s) 202, 291, 1514, 1780
AxyI CCTNAGG 1 cut(s) 537
BaeGI GKGCMC 1 cut(s) 716
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BalI TGGCCA 1 cut(s) 333
BamHI GGATCC 1 cut(s) 1862
BanI GGYRCC 1 cut(s) 1466
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 2 cut(s) 174, 1349
Bbv12I GWGCWC 1 cut(s) 364
BbvCI CCTCAGC 1 cut(s) 878
BccI CCATC 5 cut(s) 103, 407, 1877, 1985, 1986
BceAI ACGGC 2 cut(s) 670, 732
BcgI CGANNNNNNTGC 6 cut(s) 703, 737, 1019, 1053, 1448, 1482
BciT130I CCWGG 2 cut(s) 496, 1860
BciVI GTATCC 2 cut(s) 1121, 1736
BclI TGATCA 1 cut(s) 981
BcnI CCSGG 1 cut(s) 823
BcoDI GTCTC 4 cut(s) 224, 1089, 1933, 1956
BfaI CTAG 6 cut(s) 606, 738, 831, 915, 1430, 1758
BfmI CTRYAG 3 cut(s) 1234, 1407, 1621
BfoI RGCGCY 1 cut(s) 120
BfuAI ACCTGC 2 cut(s) 1343, 1624
BfuI GTATCC 2 cut(s) 1121, 1736
BglI GCCNNNNNGGC 1 cut(s) 128
BlpI GCTNAGC 1 cut(s) 1479
Bme1390I CCNGG 3 cut(s) 496, 823, 1860
Bme18I GGWCC 4 cut(s) 202, 291, 1514, 1780
BmgBI CACGTC 1 cut(s) 730
BmgT120I GGNCC 5 cut(s) 202, 291, 632, 1514, 1780
BmiI GGNNCC 8 cut(s) 245, 293, 1423, 1468, 1516, 1614, 1864, 1976
BmrFI CCNGG 3 cut(s) 496, 823, 1860
BmsI GCATC 7 cut(s) 864, 1051, 1107, 1434, 1553, 1555, 1663
BpiI GAAGAC 2 cut(s) 174, 1349
Bpu10I CCTNAGC 2 cut(s) 878, 1181
Bpu1102I GCTNAGC 1 cut(s) 1479
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 1 cut(s) 823
BsaAI YACGTR 1 cut(s) 423
BsaBI GATNNNNATC 1 cut(s) 1497
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 30, 574
BsaWI WCCGGW 3 cut(s) 288, 1080, 1872
Bsc4I CCNNNNNNNGG 6 cut(s) 199, 253, 942, 1140, 1626, 1872
Bse118I RCCGGY 1 cut(s) 1080
Bse1I ACTGG 5 cut(s) 176, 334, 592, 936, 1129
Bse21I CCTNAGG 1 cut(s) 537
Bse8I GATNNNNATC 1 cut(s) 1497
BseAI TCCGGA 1 cut(s) 1872
BseBI CCWGG 2 cut(s) 496, 1860
BseDI CCNNGG 2 cut(s) 30, 574
BseGI GGATG 7 cut(s) 189, 366, 399, 1261, 1449, 1710, 1869
BseJI GATNNNNATC 1 cut(s) 1497
BseLI CCNNNNNNNGG 6 cut(s) 199, 253, 942, 1140, 1626, 1872
BseMII CTCAG 5 cut(s) 162, 528, 848, 869, 1470
BseNI ACTGG 5 cut(s) 176, 334, 592, 936, 1129
BseSI GKGCMC 1 cut(s) 716
BseYI CCCAGC 2 cut(s) 629, 1134
BsgI GTGCAG 2 cut(s) 960, 1181
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 6 cut(s) 35, 333, 347, 634, 747, 1347
BshNI GGYRCC 1 cut(s) 1466
BshTI ACCGGT 1 cut(s) 1080
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 5 cut(s) 128, 289, 822, 1081, 1873
BsiWI CGTACG 1 cut(s) 423
BslI CCNNNNNNNGG 6 cut(s) 199, 253, 942, 1140, 1626, 1872
BsmAI GTCTC 4 cut(s) 224, 1089, 1933, 1956
BsmBI CGTCTC 1 cut(s) 1933
BsmI GAATGC 2 cut(s) 814, 1648
BsnI GGCC 6 cut(s) 35, 333, 347, 634, 747, 1347
Bsp1286I GDGCHC 3 cut(s) 246, 364, 716
Bsp13I TCCGGA 1 cut(s) 1872
Bsp1407I TGTACA 1 cut(s) 297
Bsp1720I GCTNAGC 1 cut(s) 1479
Bsp19I CCATGG 1 cut(s) 574
BspACI CCGC 4 cut(s) 211, 435, 687, 1682
BspANI GGCC 6 cut(s) 35, 333, 347, 634, 747, 1347
BspCNI CTCAG 5 cut(s) 161, 529, 849, 870, 1471
BspEI TCCGGA 1 cut(s) 1872
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 8 cut(s) 245, 293, 1423, 1468, 1516, 1614, 1864, 1976
BspMAI CTGCAG 1 cut(s) 1411
BspMI ACCTGC 2 cut(s) 1343, 1624
BspPI GGATC 4 cut(s) 1493, 1857, 1870, 1883
BspT107I GGYRCC 1 cut(s) 1466
BsrFI RCCGGY 1 cut(s) 1080
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 5 cut(s) 176, 334, 592, 936, 1129
BssAI RCCGGY 1 cut(s) 1080
BssECI CCNNGG 2 cut(s) 30, 574
BssNI GRCGYC 1 cut(s) 450
BssSI CACGAG 1 cut(s) 722
BssT1I CCWWGG 2 cut(s) 30, 574
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 2 cut(s) 496, 1860
Bst4CI ACNGT 4 cut(s) 928, 1159, 1266, 1817
Bst6I CTCTTC 2 cut(s) 1029, 1775
BstACI GRCGYC 1 cut(s) 450
BstAUI TGTACA 1 cut(s) 297
BstBAI YACGTR 1 cut(s) 423
BstC8I GCNNGC 7 cut(s) 611, 659, 828, 852, 1246, 1485, 1827
BstDEI CTNAG 6 cut(s) 148, 537, 857, 878, 1181, 1479
BstDSI CCRYGG 1 cut(s) 574
BstEII GGTNACC 1 cut(s) 490
BstF5I GGATG 7 cut(s) 189, 366, 399, 1261, 1449, 1710, 1869
BstFNI CGCG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 4 cut(s) 106, 119, 272, 1843
BstMAI GTCTC 4 cut(s) 224, 1089, 1933, 1956
BstNI CCWGG 2 cut(s) 496, 1860
BstNSI RCATGY 4 cut(s) 830, 854, 1036, 1657
BstPI GGTNACC 1 cut(s) 490
BstSCI CCNGG 3 cut(s) 494, 821, 1858
BstSFI CTRYAG 3 cut(s) 1234, 1407, 1621
BstSLI GKGCMC 1 cut(s) 716
BstUI CGCG 1 cut(s) 270
BstV2I GAAGAC 2 cut(s) 174, 1349
BstX2I RGATCY 1 cut(s) 1862
BstXI CCANNNNNNTGG 1 cut(s) 1314
BstYI RGATCY 1 cut(s) 1862
Bsu36I CCTNAGG 1 cut(s) 537
BsuI GTATCC 2 cut(s) 1121, 1736
BsuRI GGCC 6 cut(s) 35, 333, 347, 634, 747, 1347
BtgI CCRYGG 1 cut(s) 574
BtrI CACGTC 1 cut(s) 730
BtsCI GGATG 7 cut(s) 189, 366, 399, 1261, 1449, 1710, 1869
BtsI GCAGTG 2 cut(s) 673, 1404
BtsIMutI CAGTG 4 cut(s) 673, 943, 1404, 1822
BveI ACCTGC 2 cut(s) 1343, 1624
Cac8I GCNNGC 7 cut(s) 611, 659, 828, 852, 1246, 1485, 1827
CfoI GCGC 4 cut(s) 106, 119, 272, 1843
Cfr10I RCCGGY 1 cut(s) 1080
Cfr13I GGNCC 5 cut(s) 202, 291, 632, 1514, 1780
CseI GACGC 2 cut(s) 458, 785
Csp6I GTAC 8 cut(s) 55, 175, 298, 321, 424, 1540, 1766, 1924
CspAI ACCGGT 1 cut(s) 1080
CspCI CAANNNNNGTGG 2 cut(s) 944, 979
CviQI GTAC 8 cut(s) 55, 175, 298, 321, 424, 1540, 1766, 1924
DdeI CTNAG 6 cut(s) 148, 537, 857, 878, 1181, 1479
EaeI YGGCCR 3 cut(s) 331, 745, 1345
Eam1104I CTCTTC 2 cut(s) 1029, 1775
EarI CTCTTC 2 cut(s) 1029, 1775
Eco130I CCWWGG 2 cut(s) 30, 574
Eco147I AGGCCT 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 202, 291, 1514, 1780
Eco47III AGCGCT 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 537
Eco91I GGTNACC 1 cut(s) 490
EcoO109I RGGNCCY 2 cut(s) 202, 1514
EcoO65I GGTNACC 1 cut(s) 490
EcoRI GAATTC 2 cut(s) 1111, 1191
EcoRII CCWGG 2 cut(s) 494, 1858
EcoT14I CCWWGG 2 cut(s) 30, 574
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 2 cut(s) 30, 574
Esp3I CGTCTC 1 cut(s) 1933
FalI AAGNNNNNCTT 2 cut(s) 1164, 1196
FauI CCCGC 1 cut(s) 1689
FauNDI CATATG 1 cut(s) 978
FbaI TGATCA 1 cut(s) 981
FokI GGATG 7 cut(s) 176, 353, 386, 1268, 1456, 1717, 1856
FriOI GRGCYC 1 cut(s) 246
FspBI CTAG 6 cut(s) 606, 738, 831, 915, 1430, 1758
GlaI GCGC 4 cut(s) 105, 118, 271, 1842
GsaI CCCAGC 2 cut(s) 633, 1138
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 6 cut(s) 35, 333, 347, 634, 747, 1347
HapII CCGG 5 cut(s) 128, 289, 822, 1081, 1873
HgaI GACGC 2 cut(s) 458, 785
HhaI GCGC 4 cut(s) 106, 119, 272, 1843
Hin1I GRCGYC 1 cut(s) 450
Hin6I GCGC 4 cut(s) 104, 117, 270, 1841
HinP1I GCGC 4 cut(s) 104, 117, 270, 1841
HindIII AAGCTT 1 cut(s) 1823
HinfI GANTC 6 cut(s) 146, 217, 227, 758, 888, 1508
HpaII CCGG 5 cut(s) 128, 289, 822, 1081, 1873
HphI GGTGA 3 cut(s) 484, 989, 1913
Hpy166II GTNNAC 7 cut(s) 597, 727, 924, 970, 997, 1651, 1780
Hpy188I TCNGA 5 cut(s) 87, 151, 160, 858, 1742
Hpy8I GTNNAC 7 cut(s) 597, 727, 924, 970, 997, 1651, 1780
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 6 cut(s) 155, 215, 404, 829, 1144, 1253
HpyCH4III ACNGT 4 cut(s) 928, 1159, 1266, 1817
HpyCH4IV ACGT 6 cut(s) 422, 426, 729, 1323, 1746, 1926
HpyF3I CTNAG 6 cut(s) 148, 537, 857, 878, 1181, 1479
HpySE526I ACGT 6 cut(s) 422, 426, 729, 1323, 1746, 1926
Hsp92I GRCGYC 1 cut(s) 450
HspAI GCGC 4 cut(s) 104, 117, 270, 1841
Kpn2I TCCGGA 1 cut(s) 1872
Ksp22I TGATCA 1 cut(s) 981
LmnI GCTCC 3 cut(s) 249, 952, 1421
LweI GCATC 7 cut(s) 864, 1051, 1107, 1434, 1553, 1555, 1663
MaeI CTAG 6 cut(s) 606, 738, 831, 915, 1430, 1758
MaeII ACGT 6 cut(s) 422, 426, 729, 1323, 1746, 1926
MaeIII GTNAC 2 cut(s) 490, 1324
MflI RGATCY 1 cut(s) 1862
MhlI GDGCHC 3 cut(s) 246, 364, 716
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 3 cut(s) 351, 1111, 1191
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 2 cut(s) 140, 226
MmeI TCCRAC 2 cut(s) 27, 1720
Mox20I TGGCCA 1 cut(s) 333
MroI TCCGGA 1 cut(s) 1872
MroXI GAANNNNTTC 1 cut(s) 1292
MscI TGGCCA 1 cut(s) 333
MseI TTAA 4 cut(s) 264, 312, 339, 1848
MslI CAYNNNNRTG 3 cut(s) 699, 1658, 1986
Msp20I TGGCCA 1 cut(s) 333
MspA1I CMGCKG 2 cut(s) 1165, 1412
MspI CCGG 5 cut(s) 128, 289, 822, 1081, 1873
MspR9I CCNGG 3 cut(s) 496, 823, 1860
Mva1269I GAATGC 2 cut(s) 814, 1648
MvaI CCWGG 2 cut(s) 496, 1860
MvnI CGCG 1 cut(s) 270
NciI CCSGG 1 cut(s) 823
NcoI CCATGG 1 cut(s) 574
NdeI CATATG 1 cut(s) 978
NlaIV GGNNCC 8 cut(s) 245, 293, 1423, 1468, 1516, 1614, 1864, 1976
NmeAIII GCCGAG 1 cut(s) 1373
NmuCI GTSAC 1 cut(s) 490
NspI RCATGY 4 cut(s) 830, 854, 1036, 1657
PaeI GCATGC 2 cut(s) 830, 854
PceI AGGCCT 1 cut(s) 35
PciI ACATGT 2 cut(s) 1032, 1653
PctI GAATGC 2 cut(s) 814, 1648
PdmI GAANNNNTTC 1 cut(s) 1292
PfeI GAWTC 4 cut(s) 227, 758, 888, 1508
Pfl23II CGTACG 1 cut(s) 423
PflMI CCANNNNNTGG 1 cut(s) 253
PfoI TCCNGGA 1 cut(s) 1858
PinAI ACCGGT 1 cut(s) 1080
PleI GAGTC 2 cut(s) 140, 225
PpsI GAGTC 2 cut(s) 140, 225
Ppu21I YACGTR 1 cut(s) 423
PpuMI RGGWCCY 2 cut(s) 202, 1514
PscI ACATGT 2 cut(s) 1032, 1653
Psp5II RGGWCCY 2 cut(s) 202, 1514
Psp6I CCWGG 2 cut(s) 494, 1858
PspEI GGTNACC 1 cut(s) 490
PspFI CCCAGC 2 cut(s) 629, 1134
PspGI CCWGG 2 cut(s) 494, 1858
PspLI CGTACG 1 cut(s) 423
PspN4I GGNNCC 8 cut(s) 245, 293, 1423, 1468, 1516, 1614, 1864, 1976
PspPI GGNCC 5 cut(s) 202, 291, 632, 1514, 1780
PspPPI RGGWCCY 2 cut(s) 202, 1514
PstI CTGCAG 1 cut(s) 1411
PsuI RGATCY 1 cut(s) 1862
PvuII CAGCTG 2 cut(s) 1165, 1412
RsaI GTAC 8 cut(s) 56, 176, 299, 322, 425, 1541, 1767, 1925
RsaNI GTAC 8 cut(s) 55, 175, 298, 321, 424, 1540, 1766, 1924
RseI CAYNNNNRTG 3 cut(s) 699, 1658, 1986
SaqAI TTAA 4 cut(s) 264, 312, 339, 1848
Sau96I GGNCC 5 cut(s) 202, 291, 632, 1514, 1780
SchI GAGTC 2 cut(s) 140, 226
ScrFI CCNGG 3 cut(s) 496, 823, 1860
SduI GDGCHC 3 cut(s) 246, 364, 716
SfaNI GCATC 7 cut(s) 864, 1051, 1107, 1434, 1553, 1555, 1663
SfcI CTRYAG 3 cut(s) 1234, 1407, 1621
SinI GGWCC 4 cut(s) 202, 291, 1514, 1780
SmiMI CAYNNNNRTG 3 cut(s) 699, 1658, 1986
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SphI GCATGC 2 cut(s) 830, 854
Sse9I AATT 3 cut(s) 351, 1111, 1191
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 4 cut(s) 211, 435, 687, 1682
SspMI CTAG 6 cut(s) 606, 738, 831, 915, 1430, 1758
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 3 cut(s) 494, 821, 1858
StyI CCWWGG 2 cut(s) 30, 574
TaaI ACNGT 4 cut(s) 928, 1159, 1266, 1817
TaiI ACGT 6 cut(s) 425, 429, 732, 1326, 1749, 1929
TaqI TCGA 6 cut(s) 465, 532, 783, 1029, 1115, 1458
TasI AATT 3 cut(s) 351, 1111, 1191
TatI WGTACW 3 cut(s) 54, 297, 1765
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 4 cut(s) 227, 758, 888, 1508
Tru1I TTAA 4 cut(s) 264, 312, 339, 1848
Tru9I TTAA 4 cut(s) 264, 312, 339, 1848
TscAI CASTG 4 cut(s) 680, 943, 1411, 1822
TseFI GTSAC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 7 cut(s) 17, 483, 656, 683, 1272, 1369, 1604
TspGWI ACGGA 2 cut(s) 347, 1976
TspRI CASTG 4 cut(s) 680, 943, 1411, 1822
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 4 cut(s) 202, 291, 1514, 1780
XapI RAATTY 2 cut(s) 1111, 1191
XceI RCATGY 4 cut(s) 830, 854, 1036, 1657
XcmI CCANNNNNNNNNTGG 2 cut(s) 706, 1142
XmnI GAANNNNTTC 1 cut(s) 1292
XspI CTAG 6 cut(s) 606, 738, 831, 915, 1430, 1758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.