RLG00000031914

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007491
Physical Location & Seq
Reverse (-)
9434333 .. 9436412
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031914

Sequence Viewer

Length: 1092 bp
ATGACAAAAGATGCACTGAAATGGTTAATGGTGATTGCTGTTTTGGTGGTTTCAATATCACAATCATGTGTTCATGGAGCGCCACAAGTACCTTGCTTCTTCATTTTCGGTGATTCATTGGCCGATAACGGCAATAACAATCTCCTATTGACTTTAGCCAAAGTGAACTATGCGCCGTACGGAATTGATTATCCCGGTGGAGTAACAACGGGAAGATTTTCCAATGGCAAAACTACTGTTGATATACTAGCTGAATTTCTGGGTTTTGAGAATCCCATCCCACCATTTGCAACTACCAACATACAGGACATTGTTAAAGGTCTCAATTATGCATCTGGTGCCGCAGGGATTCGAGCAGAAACTGGCTCCAATTTGGGTGGTCACGTCAGCTTAGACCGGCAGTTACTGAATCACCAAATCACAATCTTGCGCATTGCTTCAATACTTCGAGCAAATACACTACAAACCTTGCAGTACTTGAACAAGTGCCTATACACAGTGGGAATGGGTAACAATGATTACATTAACAATTACTTCATGCCTCAGTTTTATCCCACCAGCAAGAGATACACCCCTGAACAATATCTTCAGTTCTTATTGACCAATATACTAGCCAAATACTGGTTAACTTTGTACAACAAGTATGGAGCAAGGAAGGTTGCCTTGGTTGGACTGGGACGCATAGGATGCATTCCTAATGCAATTTCGAAAACAAAGGGGATCACATGTGTAGATGAATTGAACAGTGCGGCCCAGCTTTTCAACCAAAAGCTTGTACCTCTTGTTGATCAGCTCAATACCAATTTGACTGATGCAAAATTCATCTATGTCAACAGTACTGGAATGAGTTCTGGGGATCCTACATCTGTTGGATTCAAGGTTTTGAATGTTGGGTGTTGTCCGGTGAATAGTGTTGGCCAATGTATTCGTTCCGGAACTCCATGCCAGAATAGGAGTGAGTATGTGTTCTGGGACTCATTCCACCCTACTGAGGCATCGAACCGAATTACTGCATTCAGAACATACCAAAGTGTTGACCCCACGGATAATTATCCTATGGATGTCAGTCACTTAGTTCAGCTTAAACTATAA

Protein Analysis

364

Amino Acids

39.93

Weight (kDa)

8.62

Isoelectric Point (pI)

27.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 338 3.6e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 621
AccIII TCCGGA 1 cut(s) 932
AciI CCGC 2 cut(s) 342, 749
AclWI GGATC 3 cut(s) 728, 851, 864
AcoI YGGCCR 2 cut(s) 120, 916
AcsI RAATTY 2 cut(s) 254, 818
AcuI CTGAAG 1 cut(s) 572
AfaI GTAC 6 cut(s) 90, 179, 476, 635, 777, 838
AfiI CCNNNNNNNGG 2 cut(s) 621, 991
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 7 cut(s) 54, 441, 481, 742, 763, 877, 886
AjiI CACGTC 1 cut(s) 385
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 6 cut(s) 251, 390, 757, 772, 793, 1081
AluI AGCT 6 cut(s) 251, 390, 757, 772, 793, 1081
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 3 cut(s) 728, 851, 864
AlwNI CAGNNNCTG 2 cut(s) 362, 406
Aor13HI TCCGGA 1 cut(s) 932
AoxI GGCC 3 cut(s) 120, 750, 916
ApoI RAATTY 2 cut(s) 254, 818
Asp700I GAANNNNTTC 2 cut(s) 217, 847
AspLEI GCGC 3 cut(s) 82, 175, 432
AspS9I GGNCC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 4 cut(s) 43, 122, 404, 916
AsuII TTCGAA 1 cut(s) 707
BalI TGGCCA 1 cut(s) 918
BamHI GGATCC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 338
BccI CCATC 1 cut(s) 284
BceAI ACGGC 2 cut(s) 145, 160
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 2 cut(s) 248, 611
BfoI RGCGCY 1 cut(s) 83
BisI GCNGC 2 cut(s) 342, 750
BlsI GCNGC 2 cut(s) 343, 751
BmcAI AGTACT 2 cut(s) 476, 838
Bme1390I CCNGG 1 cut(s) 195
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 3 cut(s) 340, 367, 858
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 1 cut(s) 683
BmsI GCATC 4 cut(s) 341, 677, 802, 1004
BmuI ACTGGG 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 1050
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 663, 1041
BsaWI WCCGGW 2 cut(s) 901, 932
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 991
Bse118I RCCGGY 1 cut(s) 396
Bse1I ACTGG 4 cut(s) 367, 626, 678, 844
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 1050
BseAI TCCGGA 1 cut(s) 932
BseDI CCNNGG 2 cut(s) 663, 1041
BseGI GGATG 3 cut(s) 276, 692, 1066
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 2 cut(s) 621, 991
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 2 cut(s) 557, 981
BseNI ACTGG 4 cut(s) 367, 626, 678, 844
BseYI CCCAGC 1 cut(s) 753
BshFI GGCC 3 cut(s) 122, 752, 918
BshNI GGYRCC 1 cut(s) 338
BsiSI CCGG 4 cut(s) 195, 397, 902, 933
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 2 cut(s) 690, 986
BslI CCNNNNNNNGG 2 cut(s) 621, 991
BsmAI GTCTC 1 cut(s) 326
BsmFI GGGAC 2 cut(s) 690, 986
BsmI GAATGC 2 cut(s) 690, 1013
BsnI GGCC 3 cut(s) 122, 752, 918
Bso31I GGTCTC 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 707
Bsp13I TCCGGA 1 cut(s) 932
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 3 cut(s) 720, 787, 856
BspACI CCGC 2 cut(s) 342, 749
BspANI GGCC 3 cut(s) 122, 752, 918
BspCNI CTCAG 2 cut(s) 556, 982
BspEI TCCGGA 1 cut(s) 932
BspLI GGNNCC 3 cut(s) 340, 367, 858
BspPI GGATC 3 cut(s) 728, 851, 864
BspT104I TTCGAA 1 cut(s) 707
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 396
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 4 cut(s) 367, 626, 678, 844
BssAI RCCGGY 1 cut(s) 396
BssECI CCNNGG 2 cut(s) 663, 1041
BssMI GATC 3 cut(s) 720, 787, 856
BssT1I CCWWGG 1 cut(s) 663
Bst4CI ACNGT 4 cut(s) 238, 499, 746, 836
BstAPI GCANNNNNTGC 2 cut(s) 338, 687
BstAUI TGTACA 1 cut(s) 633
BstBI TTCGAA 1 cut(s) 707
BstDEI CTNAG 4 cut(s) 391, 543, 990, 1072
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 3 cut(s) 276, 692, 1066
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 3 cut(s) 82, 175, 432
BstKTI GATC 3 cut(s) 723, 790, 859
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 3 cut(s) 720, 787, 856
BstMWI GCNNNNNNNGC 2 cut(s) 338, 687
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
BsuRI GGCC 3 cut(s) 122, 752, 918
BtgI CCRYGG 1 cut(s) 1041
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 3 cut(s) 276, 692, 1066
BtsIMutI CAGTG 3 cut(s) 14, 504, 751
CaiI CAGNNNCTG 2 cut(s) 362, 406
CfoI GCGC 3 cut(s) 82, 175, 432
Cfr10I RCCGGY 1 cut(s) 396
Cfr13I GGNCC 1 cut(s) 751
CseI GACGC 1 cut(s) 687
Csp6I GTAC 6 cut(s) 89, 178, 475, 634, 776, 837
CspCI CAANNNNNGTGG 2 cut(s) 358, 393
CviAII CATG 5 cut(s) 66, 74, 538, 726, 942
CviQI GTAC 6 cut(s) 89, 178, 475, 634, 776, 837
DdeI CTNAG 4 cut(s) 391, 543, 990, 1072
DpnI GATC 3 cut(s) 722, 789, 858
DpnII GATC 3 cut(s) 720, 787, 856
EaeI YGGCCR 2 cut(s) 120, 916
Eco130I CCWWGG 1 cut(s) 663
Eco31I GGTCTC 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 572
EcoT14I CCWWGG 1 cut(s) 663
EcoT22I ATGCAT 2 cut(s) 334, 692
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 5 cut(s) 69, 77, 541, 729, 945
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 2 cut(s) 690, 986
FatI CATG 5 cut(s) 65, 73, 537, 725, 941
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 342, 750
FokI GGATG 3 cut(s) 263, 699, 1073
Fsp4HI GCNGC 2 cut(s) 342, 750
FspBI CTAG 2 cut(s) 248, 611
FspI TGCGCA 1 cut(s) 431
GlaI GCGC 3 cut(s) 81, 174, 431
GluI GCNGC 2 cut(s) 342, 750
GsaI CCCAGC 1 cut(s) 757
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 3 cut(s) 122, 752, 918
HapII CCGG 4 cut(s) 195, 397, 902, 933
HgaI GACGC 1 cut(s) 687
HhaI GCGC 3 cut(s) 82, 175, 432
Hin1II CATG 5 cut(s) 69, 77, 541, 729, 945
Hin6I GCGC 3 cut(s) 80, 173, 430
HinP1I GCGC 3 cut(s) 80, 173, 430
HincII GTYRAC 3 cut(s) 627, 832, 1036
HindII GTYRAC 3 cut(s) 627, 832, 1036
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 6 cut(s) 113, 271, 349, 409, 873, 974
HpaI GTTAAC 1 cut(s) 627
HpaII CCGG 4 cut(s) 195, 397, 902, 933
HphI GGTGA 4 cut(s) 43, 122, 404, 916
Hpy166II GTNNAC 4 cut(s) 166, 627, 832, 1036
Hpy188I TCNGA 1 cut(s) 1019
Hpy188III TCNNGA 1 cut(s) 933
Hpy8I GTNNAC 4 cut(s) 166, 627, 832, 1036
HpyAV CCTTC 1 cut(s) 649
HpyCH4III ACNGT 4 cut(s) 238, 499, 746, 836
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 8 cut(s) 14, 290, 332, 472, 690, 701, 815, 1013
HpyF10VI GCNNNNNNNGC 2 cut(s) 338, 687
HpyF3I CTNAG 4 cut(s) 391, 543, 990, 1072
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 5 cut(s) 69, 77, 541, 729, 945
HspAI GCGC 3 cut(s) 80, 173, 430
Kpn2I TCCGGA 1 cut(s) 932
Ksp22I TGATCA 1 cut(s) 787
KspAI GTTAAC 1 cut(s) 627
Kzo9I GATC 3 cut(s) 720, 787, 856
LmnI GCTCC 3 cut(s) 77, 371, 647
LweI GCATC 4 cut(s) 341, 677, 802, 1004
MaeI CTAG 2 cut(s) 248, 611
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 5 cut(s) 202, 380, 402, 509, 1067
MalI GATC 3 cut(s) 722, 789, 858
MboI GATC 3 cut(s) 720, 787, 856
MboII GAAGA 3 cut(s) 91, 225, 578
MflI RGATCY 1 cut(s) 856
MlsI TGGCCA 1 cut(s) 918
MluNI TGGCCA 1 cut(s) 918
MlyI GAGTC 1 cut(s) 968
MmeI TCCRAC 2 cut(s) 649, 850
MnlI CCTC 3 cut(s) 552, 789, 985
Mox20I TGGCCA 1 cut(s) 918
Mph1103I ATGCAT 2 cut(s) 334, 692
MroI TCCGGA 1 cut(s) 932
MroXI GAANNNNTTC 2 cut(s) 217, 847
MscI TGGCCA 1 cut(s) 918
MseI TTAA 5 cut(s) 26, 315, 525, 626, 1083
MslI CAYNNNNRTG 2 cut(s) 19, 64
Msp20I TGGCCA 1 cut(s) 918
MspI CCGG 4 cut(s) 195, 397, 902, 933
MspR9I CCNGG 1 cut(s) 195
Mva1269I GAATGC 2 cut(s) 690, 1013
MwoI GCNNNNNNNGC 2 cut(s) 338, 687
NciI CCSGG 1 cut(s) 195
NdeII GATC 3 cut(s) 720, 787, 856
NlaIII CATG 5 cut(s) 69, 77, 541, 729, 945
NlaIV GGNNCC 3 cut(s) 340, 367, 858
NmuCI GTSAC 2 cut(s) 380, 1067
NsbI TGCGCA 1 cut(s) 431
NsiI ATGCAT 2 cut(s) 334, 692
NspI RCATGY 1 cut(s) 729
NspV TTCGAA 1 cut(s) 707
PciI ACATGT 1 cut(s) 725
PctI GAATGC 2 cut(s) 690, 1013
PdmI GAANNNNTTC 2 cut(s) 217, 847
PfeI GAWTC 5 cut(s) 113, 271, 349, 409, 873
Pfl23II CGTACG 1 cut(s) 177
PflMI CCANNNNNTGG 1 cut(s) 621
PkrI GCNGC 2 cut(s) 343, 751
PleI GAGTC 1 cut(s) 968
PpsI GAGTC 1 cut(s) 968
PscI ACATGT 1 cut(s) 725
PspFI CCCAGC 1 cut(s) 753
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 340, 367, 858
PspPI GGNCC 1 cut(s) 751
PstNI CAGNNNCTG 2 cut(s) 362, 406
PsuI RGATCY 1 cut(s) 856
RsaI GTAC 6 cut(s) 90, 179, 476, 635, 777, 838
RsaNI GTAC 6 cut(s) 89, 178, 475, 634, 776, 837
RseI CAYNNNNRTG 2 cut(s) 19, 64
SaqAI TTAA 5 cut(s) 26, 315, 525, 626, 1083
SatI GCNGC 2 cut(s) 342, 750
Sau3AI GATC 3 cut(s) 720, 787, 856
Sau96I GGNCC 1 cut(s) 751
ScaI AGTACT 2 cut(s) 476, 838
SchI GAGTC 1 cut(s) 968
ScrFI CCNGG 1 cut(s) 195
SfaNI GCATC 4 cut(s) 341, 677, 802, 1004
SfuI TTCGAA 1 cut(s) 707
SmiMI CAYNNNNRTG 2 cut(s) 19, 64
SsiI CCGC 2 cut(s) 342, 749
SspMI CTAG 2 cut(s) 248, 611
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 4 cut(s) 238, 499, 746, 836
TaiI ACGT 1 cut(s) 387
TaqI TCGA 4 cut(s) 352, 448, 707, 998
TatI WGTACW 3 cut(s) 474, 633, 836
TauI GCSGC 2 cut(s) 344, 752
TfiI GAWTC 5 cut(s) 113, 271, 349, 409, 873
Tru1I TTAA 5 cut(s) 26, 315, 525, 626, 1083
Tru9I TTAA 5 cut(s) 26, 315, 525, 626, 1083
TscAI CASTG 3 cut(s) 21, 504, 751
TseFI GTSAC 2 cut(s) 380, 1067
Tsp45I GTSAC 2 cut(s) 380, 1067
TspDTI ATGAA 6 cut(s) 62, 91, 105, 526, 750, 811
TspGWI ACGGA 2 cut(s) 195, 1058
TspRI CASTG 3 cut(s) 21, 504, 751
Van91I CCANNNNNTGG 1 cut(s) 621
XapI RAATTY 2 cut(s) 254, 818
XceI RCATGY 1 cut(s) 729
XmnI GAANNNNTTC 2 cut(s) 217, 847
XspI CTAG 2 cut(s) 248, 611
ZrmI AGTACT 2 cut(s) 476, 838
Zsp2I ATGCAT 2 cut(s) 334, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.