MD10G1314100.v1.1

NDR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006390
Physical Location & Seq
Reverse (-)
39836230 .. 39836811
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1314100.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTGCGAAACAAAGAATTTCTACTGCTGGGCTCTGCAATTTCTAGCTCTCCTGGGACTCCTAGCCCTCTGTCTATGGCTGGCCTTACGTCCGAAGGACCCTCAGTATACCATTGTTGATATCAACATCCCAAAATCCGATTTAGAGGACGGCGCATACAATGGTTCTATCTTATATGCCCTTCAAATTGAAAATCCTAACAAAGACTCTAGTATTTTCTATGATGAAACAGTTTTAACGTTCTTGTATGGGTCGGATAGTGTTGGGGAGAAACACATACCAGCTTTTCATCAAGGAAGAGGTAAAACCCGTCAAGTGATTGACTATGTAGATGTCAACCCACGAGTTTGGAAAACTATTCGCAATTCCCTATTGCATGCAACTGCAGAATTGAAGGTGGCTTTGCTTTCTCGGGTTCGGTATAAAACATGGGGTGTGAAGAGTAAGCATCATGGGCTAGACTTGCAAGGTAAATTATCAATTGGTTCAGATGGTAAGATTTCTGGTAAGAAGAAAAAAATTAAGCTAAGCCATTCTTCCAAGAAATGGAGAAGGAGCAATAGCCGACGATTACTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

194

Amino Acids

21.99

Weight (kDa)

9.71

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 61 - 156 2.8e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 546
AccI GTMKAC 1 cut(s) 107
AclI AACGTT 1 cut(s) 239
AcsI RAATTY 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 546
AgsI TTSAA 3 cut(s) 185, 191, 394
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 3 cut(s) 47, 284, 526
AluI AGCT 3 cut(s) 47, 284, 526
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 1 cut(s) 81
ApoI RAATTY 1 cut(s) 16
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 34
BauI CACGAG 1 cut(s) 342
BccI CCATC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 53
BfaI CTAG 5 cut(s) 44, 62, 210, 458, 575
BfmI CTRYAG 1 cut(s) 384
BlpI GCTNAGC 1 cut(s) 527
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 457
Bpu1102I GCTNAGC 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 52
Bsc4I CCNNNNNNNGG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 116
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 411
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 1 cut(s) 83
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 34
Bsp1720I GCTNAGC 1 cut(s) 527
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 1 cut(s) 99
BspMAI CTGCAG 1 cut(s) 388
BssECI CCNNGG 1 cut(s) 52
BssNAI GTATAC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 342
Bst1107I GTATAC 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 2 cut(s) 292, 434
BstC8I GCNNGC 2 cut(s) 81, 378
BstDEI CTNAG 2 cut(s) 102, 527
BstF5I GGATG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 454, 463
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 51
BstSFI CTRYAG 1 cut(s) 384
BstXI CCANNNNNNTGG 1 cut(s) 348
BstZ17I GTATAC 1 cut(s) 108
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 126
Cac8I GCNNGC 2 cut(s) 81, 378
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 97
CviAII CATG 3 cut(s) 377, 429, 452
DdeI CTNAG 2 cut(s) 102, 527
Eam1104I CTCTTC 2 cut(s) 292, 434
EarI CTCTTC 2 cut(s) 292, 434
Eco24I GRGCYC 1 cut(s) 34
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 51
EcoRV GATATC 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 34
FaeI CATG 3 cut(s) 380, 432, 455
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FaqI GGGAC 1 cut(s) 69
FatI CATG 3 cut(s) 376, 428, 451
FblI GTMKAC 1 cut(s) 107
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 34
FspBI CTAG 5 cut(s) 44, 62, 210, 458, 575
GlaI GCGC 1 cut(s) 154
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 83
HhaI GCGC 1 cut(s) 155
Hin1II CATG 3 cut(s) 380, 432, 455
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 2 cut(s) 57, 206
Hpy166II GTNNAC 2 cut(s) 108, 337
Hpy188I TCNGA 4 cut(s) 93, 139, 256, 490
Hpy8I GTNNAC 2 cut(s) 108, 337
Hpy99I CGWCG 1 cut(s) 570
HpyAV CCTTC 4 cut(s) 88, 191, 388, 546
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 2 cut(s) 88, 239
HpyCH4V TGCA 5 cut(s) 37, 376, 380, 386, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 454, 463
HpyF3I CTNAG 2 cut(s) 102, 527
HpySE526I ACGT 2 cut(s) 88, 239
Hsp92II CATG 3 cut(s) 380, 432, 455
HspAI GCGC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 555
LpnPI CCDG 6 cut(s) 13, 38, 65, 65, 294, 489
LweI GCATC 1 cut(s) 457
MaeI CTAG 5 cut(s) 44, 62, 210, 458, 575
MaeII ACGT 2 cut(s) 88, 239
MboII GAAGA 4 cut(s) 309, 451, 523, 528
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 8 cut(s) 16, 38, 186, 364, 389, 473, 480, 519
MlyI GAGTC 2 cut(s) 51, 200
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 4 cut(s) 77, 111, 139, 293
MseI TTAA 2 cut(s) 236, 522
MspR9I CCNGG 1 cut(s) 53
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 454, 463
NlaIII CATG 3 cut(s) 380, 432, 455
NlaIV GGNNCC 1 cut(s) 99
NspI RCATGY 1 cut(s) 380
PaeI GCATGC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 546
PleI GAGTC 2 cut(s) 51, 200
PpsI GAGTC 2 cut(s) 51, 200
PpuMI RGGWCCY 1 cut(s) 97
Psp1406I AACGTT 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 97
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 388
SaqAI TTAA 2 cut(s) 236, 522
Sau96I GGNCC 1 cut(s) 97
SchI GAGTC 2 cut(s) 51, 200
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 1 cut(s) 34
SetI ASST 8 cut(s) 49, 91, 242, 286, 304, 399, 472, 528
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 1 cut(s) 384
SinI GGWCC 1 cut(s) 97
SphI GCATGC 1 cut(s) 380
Sse9I AATT 8 cut(s) 16, 38, 186, 364, 389, 473, 480, 519
SspMI CTAG 5 cut(s) 44, 62, 210, 458, 575
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 2 cut(s) 91, 242
TasI AATT 8 cut(s) 16, 38, 186, 364, 389, 473, 480, 519
Tru1I TTAA 2 cut(s) 236, 522
Tru9I TTAA 2 cut(s) 236, 522
TspDTI ATGAA 2 cut(s) 240, 278
Van91I CCANNNNNTGG 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 1 cut(s) 16
XceI RCATGY 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 107
XspI CTAG 5 cut(s) 44, 62, 210, 458, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.