Rroxscaffold_1G00071910

NDR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006390
Physical Location & Seq
Reverse (-)
93037784 .. 93040139
2356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071910.1

Sequence Viewer

Length: 1002 bp
ATGTGCGAAAGCGAAAACAAGCATTTCTATTTGTGTGTCTTAGAAATTTTGGCCCTTTTGGGTCTTCTGGCCTTAGTTCTTTGGCTAAGTTTACGCCCAAAGTCCCCTTGTTATACCATTGTGGATATCACCATTCCAAATTCTGATATAGAGAATGGGGCACAGAATGGCTCTATTGTGTATGTTCTTGAGATTGAAAACTCTAACAAAGACTCCAGTATTTTTTATGATACCTCAATCTTGACGTTCTATTATGGGCAGGATAAAGTTGCAGAGAAAACCATACCTCCTTTTCATCAAGGAAGAAGCAAAACCCGTACAGTGATGGATGATATTGATGCCAATCCACAGGTGTGGAGAGCTGTTCGCAATTCAATATCGAATGCAACTGCAGAGTTGAAGGTGACTTTGCTAACTAAGATTAGATACAAAACATGGGGAATCAAGAGTAAGCGTCACGGGCAAGACTTGCAAGGTGCATTACCAATAGGTAAAGACGGCAAGCTTTCTGGTAAGAAGAAGAAAATCAAGCTAAGCCATGCTTCCAAGAAACTGAGGAGGAATACTTTCTCTATTGGTTTTGTCTTTTTGAAAAGGAATGCCCAGCTAGTATCCCCAAGTTCCCAACCATGTACTGCAGAAGCAAAGTTTCAAGTCCAGCACGGATTTCCTGCAGCAAAGGGTAAAGTTGCAGAGAAAACCATACCTCCTTTTCATCAAGGAACTGGCAGAAGCAGACGTCGTACAGTGATTGATTATATGGATGCCAATCCACAAGTGTGGAGAGCTGTTCGCAAATCAATATCGAATGCAAGTGCAGAATTGAAGGTGGGTTTACTAACTAGGATTCGATACACAACATGGGGAACCAAGAGGCATCATGTGCTAGACTTGCAAGGTTTGTTACCAATAGGTAAAGACGGCAAGCTTTCTAGTAAGAAGAAGAAAATCAAGCTAAGATATGCTTCCAAGAAACTGAGGACGAATAGTCTTCGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

333

Amino Acids

37.54

Weight (kDa)

10.18

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 63 - 153 7.8e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 742
AcsI RAATTY 2 cut(s) 45, 139
AcyI GRCGYC 1 cut(s) 739
AfaI GTAC 3 cut(s) 319, 634, 745
AgsI TTSAA 6 cut(s) 197, 375, 400, 592, 653, 826
AhdI GACNNNNNGTC 1 cut(s) 987
AleI CACNNNNGTG 2 cut(s) 352, 778
AluBI AGCT 7 cut(s) 362, 505, 532, 607, 788, 928, 955
AluI AGCT 7 cut(s) 362, 505, 532, 607, 788, 928, 955
AoxI GGCC 2 cut(s) 51, 69
ApeKI GCWGC 1 cut(s) 674
ApoI RAATTY 2 cut(s) 45, 139
Asp700I GAANNNNTTC 1 cut(s) 566
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 121, 415
BaeGI GKGCMC 1 cut(s) 163
BbsI GAAGAC 2 cut(s) 56, 983
BbvI GCAGC 1 cut(s) 686
BccI CCATC 1 cut(s) 319
BceAI ACGGC 2 cut(s) 514, 937
BciVI GTATCC 1 cut(s) 622
BfaI CTAG 4 cut(s) 608, 843, 887, 933
BfmI CTRYAG 3 cut(s) 390, 636, 672
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 1 cut(s) 675
BlpI GCTNAGC 1 cut(s) 533
BlsI GCNGC 1 cut(s) 676
BmeRI GACNNNNNGTC 1 cut(s) 987
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 868
BmsI GCATC 3 cut(s) 328, 754, 886
BpiI GAAGAC 2 cut(s) 56, 983
BpmI CTGGAG 1 cut(s) 199
Bpu1102I GCTNAGC 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 209
BsaBI GATNNNNATC 2 cut(s) 342, 768
BsaHI GRCGYC 1 cut(s) 739
BsaXI ACNNNNNCTCC 6 cut(s) 197, 227, 271, 301, 691, 721
Bse1I ACTGG 2 cut(s) 216, 730
Bse8I GATNNNNATC 2 cut(s) 342, 768
BseGI GGATG 2 cut(s) 334, 769
BseJI GATNNNNATC 2 cut(s) 342, 768
BseMII CTCAG 2 cut(s) 545, 968
BseNI ACTGG 2 cut(s) 216, 730
BseRI GAGGAG 1 cut(s) 571
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 1 cut(s) 686
BseYI CCCAGC 1 cut(s) 603
BsgI GTGCAG 1 cut(s) 837
BshFI GGCC 2 cut(s) 53, 71
BslFI GGGAC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 88
BsmI GAATGC 3 cut(s) 388, 604, 814
BsnI GGCC 2 cut(s) 53, 71
Bsp1286I GDGCHC 1 cut(s) 163
Bsp1720I GCTNAGC 1 cut(s) 533
BspANI GGCC 2 cut(s) 53, 71
BspCNI CTCAG 2 cut(s) 546, 969
BspLI GGNNCC 1 cut(s) 868
BspMAI CTGCAG 3 cut(s) 394, 640, 676
BsrI ACTGG 2 cut(s) 216, 730
BssNI GRCGYC 1 cut(s) 739
Bst4CI ACNGT 2 cut(s) 322, 748
BstACI GRCGYC 1 cut(s) 739
BstAPI GCANNNNNTGC 2 cut(s) 469, 883
BstC8I GCNNGC 2 cut(s) 503, 926
BstDEI CTNAG 8 cut(s) 40, 73, 86, 417, 533, 554, 956, 977
BstF5I GGATG 2 cut(s) 334, 769
BstMWI GCNNNNNNNGC 4 cut(s) 460, 469, 883, 892
BstSFI CTRYAG 3 cut(s) 390, 636, 672
BstSLI GKGCMC 1 cut(s) 163
BstV1I GCAGC 1 cut(s) 686
BstV2I GAAGAC 2 cut(s) 56, 983
BstXI CCANNNNNNTGG 2 cut(s) 354, 780
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 53, 71
BtsCI GGATG 2 cut(s) 334, 769
BtsIMutI CAGTG 2 cut(s) 327, 753
Cac8I GCNNGC 2 cut(s) 503, 926
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 443
Csp6I GTAC 3 cut(s) 318, 633, 744
CviAII CATG 5 cut(s) 435, 539, 630, 861, 881
CviQI GTAC 3 cut(s) 318, 633, 744
DdeI CTNAG 8 cut(s) 40, 73, 86, 417, 533, 554, 956, 977
DriI GACNNNNNGTC 1 cut(s) 987
Eam1105I GACNNNNNGTC 1 cut(s) 987
Eco32I GATATC 1 cut(s) 127
EcoRV GATATC 1 cut(s) 127
FaeI CATG 5 cut(s) 438, 542, 633, 864, 884
FalI AAGNNNNNCTT 4 cut(s) 526, 558, 949, 981
FaqI GGGAC 1 cut(s) 88
FatI CATG 5 cut(s) 434, 538, 629, 860, 880
Fnu4HI GCNGC 1 cut(s) 675
FokI GGATG 2 cut(s) 341, 776
Fsp4HI GCNGC 1 cut(s) 675
FspBI CTAG 4 cut(s) 608, 843, 887, 933
GluI GCNGC 1 cut(s) 675
GsaI CCCAGC 1 cut(s) 607
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 2 cut(s) 53, 71
HgaI GACGC 1 cut(s) 443
Hin1I GRCGYC 1 cut(s) 739
Hin1II CATG 5 cut(s) 438, 542, 633, 864, 884
HindIII AAGCTT 2 cut(s) 503, 926
HinfI GANTC 3 cut(s) 212, 441, 847
HphI GGTGA 2 cut(s) 121, 415
Hpy166II GTNNAC 2 cut(s) 92, 836
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 3 cut(s) 188, 241, 445
Hpy8I GTNNAC 2 cut(s) 92, 836
Hpy99I CGWCG 1 cut(s) 744
HpyAV CCTTC 2 cut(s) 394, 820
HpyCH4III ACNGT 2 cut(s) 322, 748
HpyCH4IV ACGT 2 cut(s) 245, 739
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 469, 883, 892
HpyF3I CTNAG 8 cut(s) 40, 73, 86, 417, 533, 554, 956, 977
HpySE526I ACGT 2 cut(s) 245, 739
Hsp92I GRCGYC 1 cut(s) 739
Hsp92II CATG 5 cut(s) 438, 542, 633, 864, 884
LpnPI CCDG 9 cut(s) 53, 229, 245, 335, 495, 617, 671, 684, 711
Lsp1109I GCAGC 1 cut(s) 686
LweI GCATC 3 cut(s) 328, 754, 886
MaeI CTAG 4 cut(s) 608, 843, 887, 933
MaeII ACGT 2 cut(s) 245, 739
MaeIII GTNAC 3 cut(s) 403, 455, 903
MboII GAAGA 7 cut(s) 56, 315, 529, 532, 952, 955, 983
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 4 cut(s) 45, 139, 370, 821
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 244, 297, 549, 552, 717, 867, 972
MroXI GAANNNNTTC 1 cut(s) 566
MslI CAYNNNNRTG 2 cut(s) 352, 778
Mva1269I GAATGC 3 cut(s) 388, 604, 814
MwoI GCNNNNNNNGC 4 cut(s) 460, 469, 883, 892
NlaIII CATG 5 cut(s) 438, 542, 633, 864, 884
NlaIV GGNNCC 1 cut(s) 868
NmuCI GTSAC 2 cut(s) 403, 455
OliI CACNNNNGTG 2 cut(s) 352, 778
PctI GAATGC 3 cut(s) 388, 604, 814
PdmI GAANNNNTTC 1 cut(s) 566
PfeI GAWTC 2 cut(s) 441, 847
PkrI GCNGC 1 cut(s) 676
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 868
PspPI GGNCC 1 cut(s) 52
PstI CTGCAG 3 cut(s) 394, 640, 676
RsaI GTAC 3 cut(s) 319, 634, 745
RsaNI GTAC 3 cut(s) 318, 633, 744
RseI CAYNNNNRTG 2 cut(s) 352, 778
SatI GCNGC 1 cut(s) 675
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 3 cut(s) 328, 754, 886
SfcI CTRYAG 3 cut(s) 390, 636, 672
SmiMI CAYNNNNRTG 2 cut(s) 352, 778
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 4 cut(s) 45, 139, 370, 821
SspMI CTAG 4 cut(s) 608, 843, 887, 933
TaaI ACNGT 2 cut(s) 322, 748
TaiI ACGT 2 cut(s) 248, 742
TaqI TCGA 4 cut(s) 380, 806, 850, 994
TasI AATT 4 cut(s) 45, 139, 370, 821
TatI WGTACW 1 cut(s) 632
TfiI GAWTC 2 cut(s) 441, 847
TscAI CASTG 2 cut(s) 327, 753
TseFI GTSAC 2 cut(s) 403, 455
TseI GCWGC 1 cut(s) 674
Tsp45I GTSAC 2 cut(s) 403, 455
TspDTI ATGAA 2 cut(s) 284, 704
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 2 cut(s) 327, 753
XapI RAATTY 2 cut(s) 45, 139
XmnI GAANNNNTTC 1 cut(s) 566
XspI CTAG 4 cut(s) 608, 843, 887, 933
ZraI GACGTC 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.