MD12G1009500.v1.1

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008003
Physical Location & Seq
Reverse (-)
992429 .. 994979
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009500.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGCAGCAAACTCAGCCCCCAACCCCGCCATCCAAACGCAACCACTCCCCTTCCCTCCTCTTCCAGTGCTCAGACCTAATCCTCCCATGGCTAACTCCACAAGAACTCGCCACCGTCTCTCTCACCTGCACCGCCCTCCGCGCAATCTCCACCTCCATCACTCTCCGCCGCGCCTCCGACGCCTCCCGATCCCTGGAACCCCATCCGATCCCGTTCCGTAACTCCGTTGATCGACACCCGTACGCCTTCTTCATCTACACCCCATCTCAAACCCCTCCTTCCTCTTCCCGATTTCCACTCCGCCAATCCTGGGGCTCCGGTTGCTCCGCGCAGGGACCGAACTCGGCGGGTGAAATCGGTGTCGAAACGGTGCGTTTGGTGGATGAGTTGGGGGAGGGCGCGGCTGGGTGTGATTGCGAGACGTGTGGGGAAGAGGGTGGTGGGTGTCCGTGTTCAGAGTTTGGTGGGTTCGACGGCGTCGTTTCGGAGTGTGGGTTGGGTTGTGGGTGTGGATTGGATTGTGGGAATCGGGTGAGTCAGAGAGGGATCGAGGTGAGGTTGAAGATTGTGAGGGATAGGACAAAAGGGTGGAGCTTGTATGCTGATCAGTTCATTCCAAAGGGTCGGTTTGTCTGTGAATATGCAGGTGAACTCTTGACAACAAAAGAAGCAAGATTGCGGCAACAAGTATATGATGAATTTGCATCAGATGGTAATTTTTCTCCTGCTCTTTTGGTTGTGAGGGAGCATCTTCCATCCAGAAAGGCGTGTTTAAGATACAATATCGATGCAACGAGAGTTGGAAATGTAGCAAGGTTCATCAACCATTCGTGTGACGGTGGTAATTTGTTCACAACACTGGTCAGAAGTTCAGGTGCTCTACTCCCACGCCTTTGCTTTTTTTCTTCAGAAGACATACAGGAAGACGAGGAGCTTACTTTTAGCTATGGTGAAGTTACGCTAAGGTCTAAGGGTTTAAAGTGCTTTTGCGGAAGCTCTTGTTGTCTTGGAAGGTTGCCGGCAGAGCAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Pre-SET SET

Protein Analysis

345

Amino Acids

37.46

Weight (kDa)

6.03

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 196 - 317 1.8e-20 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 134, 635
Acc36I ACCTGC 2 cut(s) 134, 635
AccII CGCG 4 cut(s) 141, 171, 329, 401
AclWI GGATC 3 cut(s) 183, 202, 554
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 1 cut(s) 891
AcyI GRCGYC 2 cut(s) 180, 477
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 193, 310
AflIII ACRYGT 1 cut(s) 422
AgsI TTSAA 1 cut(s) 562
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 2 cut(s) 192, 308
AluBI AGCT 4 cut(s) 594, 934, 945, 996
AluI AGCT 4 cut(s) 594, 934, 945, 996
Alw21I GWGCWC 2 cut(s) 71, 880
Alw26I GTCTC 2 cut(s) 121, 413
AlwI GGATC 3 cut(s) 183, 202, 554
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 698
AspLEI GCGC 4 cut(s) 143, 173, 331, 401
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 6 cut(s) 115, 362, 544, 565, 659, 962
AvaII GGWCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 317
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BbsI GAAGAC 2 cut(s) 918, 930
Bbv12I GWGCWC 2 cut(s) 71, 880
BbvI GCAGC 1 cut(s) 16
BccI CCATC 6 cut(s) 37, 164, 210, 271, 704, 763
BceAI ACGGC 1 cut(s) 490
BciT130I CCWGG 2 cut(s) 194, 310
BclI TGATCA 1 cut(s) 604
BcoDI GTCTC 2 cut(s) 121, 413
BfuAI ACCTGC 2 cut(s) 134, 635
BisI GCNGC 4 cut(s) 5, 169, 402, 680
BlsI GCNGC 4 cut(s) 6, 170, 403, 681
Bme1390I CCNGG 2 cut(s) 194, 310
Bme18I GGWCC 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 3 cut(s) 198, 316, 336
BmrFI CCNGG 2 cut(s) 194, 310
BmsI GCATC 3 cut(s) 713, 757, 778
BpiI GAAGAC 2 cut(s) 918, 930
Bpu10I CCTNAGC 1 cut(s) 962
Bsa29I ATCGAT 1 cut(s) 786
BsaHI GRCGYC 2 cut(s) 180, 477
BsaJI CCNNGG 3 cut(s) 86, 192, 309
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 310
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 2 cut(s) 64, 864
BseBI CCWGG 2 cut(s) 194, 310
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 3 cut(s) 86, 192, 309
BseGI GGATG 4 cut(s) 29, 202, 388, 755
BseLI CCNNNNNNNGG 2 cut(s) 193, 310
BseMII CTCAG 2 cut(s) 26, 84
BseNI ACTGG 2 cut(s) 64, 864
BseRI GAGGAG 2 cut(s) 47, 944
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 4 cut(s) 141, 171, 329, 401
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 2 cut(s) 71, 880
BsiSI CCGG 2 cut(s) 318, 1019
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 193, 310
BsmAI GTCTC 2 cut(s) 121, 413
BsmBI CGTCTC 2 cut(s) 121, 413
BsmFI GGGAC 1 cut(s) 348
Bsp1286I GDGCHC 3 cut(s) 71, 317, 880
Bsp143I GATC 5 cut(s) 188, 207, 229, 546, 604
Bsp19I CCATGG 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 25, 83
BspDI ATCGAT 1 cut(s) 786
BspFNI CGCG 4 cut(s) 141, 171, 329, 401
BspLI GGNNCC 3 cut(s) 198, 316, 336
BspMI ACCTGC 2 cut(s) 134, 635
BspPI GGATC 3 cut(s) 183, 202, 554
BsrFI RCCGGY 1 cut(s) 1018
BsrI ACTGG 2 cut(s) 64, 864
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 3 cut(s) 86, 192, 309
BssMI GATC 5 cut(s) 188, 207, 229, 546, 604
BssNI GRCGYC 2 cut(s) 180, 477
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 194, 310
Bst4CI ACNGT 3 cut(s) 115, 370, 839
Bst6I CTCTTC 3 cut(s) 65, 289, 426
BstACI GRCGYC 2 cut(s) 180, 477
BstC8I GCNNGC 1 cut(s) 1020
BstDEI CTNAG 5 cut(s) 12, 70, 962, 969, 1032
BstDSI CCRYGG 1 cut(s) 86
BstF5I GGATG 4 cut(s) 29, 202, 388, 755
BstFNI CGCG 4 cut(s) 141, 171, 329, 401
BstHHI GCGC 4 cut(s) 143, 173, 331, 401
BstKTI GATC 5 cut(s) 191, 210, 232, 549, 607
BstMAI GTCTC 2 cut(s) 121, 413
BstMBI GATC 5 cut(s) 188, 207, 229, 546, 604
BstMWI GCNNNNNNNGC 5 cut(s) 13, 140, 179, 321, 1024
BstNI CCWGG 2 cut(s) 194, 310
BstSCI CCNGG 2 cut(s) 192, 308
BstUI CGCG 4 cut(s) 141, 171, 329, 401
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 2 cut(s) 918, 930
Bsu15I ATCGAT 1 cut(s) 786
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 1 cut(s) 86
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 4 cut(s) 29, 202, 388, 755
BtsIMutI CAGTG 2 cut(s) 71, 857
BveI ACCTGC 2 cut(s) 134, 635
Cac8I GCNNGC 1 cut(s) 1020
CfoI GCGC 4 cut(s) 143, 173, 331, 401
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 2 cut(s) 188, 466
Csp6I GTAC 1 cut(s) 241
CviAII CATG 1 cut(s) 87
CviJI RGCY 8 cut(s) 16, 91, 315, 404, 594, 934, 945, 996
CviKI_1 RGCY 8 cut(s) 16, 91, 315, 404, 594, 934, 945, 996
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 5 cut(s) 12, 70, 962, 969, 1032
DpnI GATC 5 cut(s) 190, 209, 231, 548, 606
DpnII GATC 5 cut(s) 188, 207, 229, 546, 604
DraI TTTAAA 1 cut(s) 978
Eam1104I CTCTTC 3 cut(s) 65, 289, 426
EarI CTCTTC 3 cut(s) 65, 289, 426
EciI GGCGGA 2 cut(s) 155, 290
Eco130I CCWWGG 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 891
EcoRII CCWGG 2 cut(s) 192, 308
EcoT14I CCWWGG 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 317
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 2 cut(s) 121, 413
FaeI CATG 1 cut(s) 90
FaiI YATR 7 cut(s) 88, 600, 642, 691, 693, 917, 948
FaqI GGGAC 1 cut(s) 348
FatI CATG 1 cut(s) 86
FauI CCCGC 2 cut(s) 33, 340
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 4 cut(s) 5, 169, 402, 680
FokI GGATG 4 cut(s) 16, 189, 395, 742
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 4 cut(s) 5, 169, 402, 680
GlaI GCGC 4 cut(s) 142, 172, 330, 400
GluI GCNGC 4 cut(s) 5, 169, 402, 680
GsaI CCCAGC 1 cut(s) 408
HapII CCGG 2 cut(s) 318, 1019
HgaI GACGC 2 cut(s) 188, 466
HhaI GCGC 4 cut(s) 143, 173, 331, 401
Hin1I GRCGYC 2 cut(s) 180, 477
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 4 cut(s) 141, 171, 329, 399
HinP1I GCGC 4 cut(s) 141, 171, 329, 399
HinfI GANTC 2 cut(s) 526, 535
HpaII CCGG 2 cut(s) 318, 1019
HphI GGTGA 6 cut(s) 115, 362, 544, 565, 659, 962
Hpy166II GTNNAC 2 cut(s) 650, 852
Hpy188I TCNGA 9 cut(s) 73, 178, 207, 457, 487, 540, 709, 866, 910
Hpy188III TCNNGA 4 cut(s) 186, 288, 655, 759
Hpy8I GTNNAC 2 cut(s) 650, 852
Hpy99I CGWCG 3 cut(s) 182, 476, 482
HpyAV CCTTC 4 cut(s) 60, 256, 288, 1005
HpyCH4III ACNGT 3 cut(s) 115, 370, 839
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 5 cut(s) 4, 129, 644, 704, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 140, 179, 321, 1024
HpyF3I CTNAG 5 cut(s) 12, 70, 962, 969, 1032
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 2 cut(s) 180, 477
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 4 cut(s) 141, 171, 329, 399
KroI GCCGGC 1 cut(s) 1018
KroNI GCCGGC 1 cut(s) 1020
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 5 cut(s) 188, 207, 229, 546, 604
LmnI GCTCC 5 cut(s) 320, 329, 591, 745, 931
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 713, 757, 778
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 3 cut(s) 218, 833, 955
MalI GATC 5 cut(s) 190, 209, 231, 548, 606
MboI GATC 5 cut(s) 188, 207, 229, 546, 604
MboII GAAGA 9 cut(s) 52, 241, 276, 443, 574, 743, 897, 923, 935
MhlI GDGCHC 3 cut(s) 71, 317, 880
MluCI AATT 3 cut(s) 698, 715, 844
MlyI GAGTC 1 cut(s) 544
MmeI TCCRAC 2 cut(s) 201, 781
MroNI GCCGGC 1 cut(s) 1018
MseI TTAA 2 cut(s) 773, 977
MslI CAYNNNNRTG 1 cut(s) 831
MspI CCGG 2 cut(s) 318, 1019
MspR9I CCNGG 2 cut(s) 194, 310
MvaI CCWGG 2 cut(s) 194, 310
MvnI CGCG 4 cut(s) 141, 171, 329, 401
MwoI GCNNNNNNNGC 5 cut(s) 13, 140, 179, 321, 1024
NaeI GCCGGC 1 cut(s) 1020
NcoI CCATGG 1 cut(s) 86
NdeII GATC 5 cut(s) 188, 207, 229, 546, 604
NgoMIV GCCGGC 1 cut(s) 1018
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 3 cut(s) 198, 316, 336
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 833
PaqCI CACCTGC 2 cut(s) 134, 635
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PdiI GCCGGC 1 cut(s) 1020
PfeI GAWTC 1 cut(s) 526
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 476
PkrI GCNGC 4 cut(s) 6, 170, 403, 681
PleI GAGTC 1 cut(s) 543
PpsI GAGTC 1 cut(s) 543
Psp6I CCWGG 2 cut(s) 192, 308
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 2 cut(s) 192, 308
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 198, 316, 336
PspPI GGNCC 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 476
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 2 cut(s) 773, 977
SatI GCNGC 4 cut(s) 5, 169, 402, 680
Sau3AI GATC 5 cut(s) 188, 207, 229, 546, 604
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 544
ScrFI CCNGG 2 cut(s) 194, 310
SduI GDGCHC 3 cut(s) 71, 317, 880
SfaNI GCATC 3 cut(s) 713, 757, 778
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 831
Sse9I AATT 3 cut(s) 698, 715, 844
StyD4I CCNGG 2 cut(s) 192, 308
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 3 cut(s) 115, 370, 839
TaiI ACGT 1 cut(s) 425
TaqI TCGA 5 cut(s) 232, 363, 471, 549, 786
TaqII GACCGA 1 cut(s) 352
TasI AATT 3 cut(s) 698, 715, 844
TauI GCSGC 3 cut(s) 171, 404, 682
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 2 cut(s) 773, 977
Tru9I TTAA 2 cut(s) 773, 977
TscAI CASTG 2 cut(s) 71, 864
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 4 cut(s) 241, 601, 711, 808
TspGWI ACGGA 3 cut(s) 206, 214, 438
TspRI CASTG 2 cut(s) 71, 864
Tth111I GACNNNGTC 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.