pycom12g00730

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008003
Physical Location & Seq
Reverse (-)
655492 .. 657686
2195 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00730.1

Sequence Viewer

Length: 1035 bp
ATGCAGCAAAATCAGCCCCCAACCCCGCCATCCAAACGCAACCACTCCCCTTCCCTACTCTTCCAGTGCTCAGACCTAATCCTCCCATGGCTGACCCCACAAGAACTCGCCACCGTCTCTCTCACCTGCACCGCCCTCCGCGCAATCTCCACCTCCATCACTCTCCGCCGCGCCTCCGACGCCTCCCGATACCTGGAACCCCATCCGATCCCATTCCATAACTCCGTCGATCGACACCCGTACGCCTTCTTCACCTACACCCCATCTCAAATCCCTCCTTCCTCTTCCCGATTTCCACTCCGCCAATCCTGGGGTTCCGGTTGCTCCACGCAGGGACCGAACTCGGTGGGTGAAATTGGCGTCGAAACGTTGCGTTTGGTGGATGAGTCGGGAGAGGGCGCGGCTGGGTGTGATTGCGAGACGTGTGGGGAGGAGGAAGGTGGGTGTCCGTGTACGGAGTTTGGTGGGTTTGACGGCGTCGTTTCGGAGTGTGGATTGGGTTGTGGGTGTGGGTTGGATTGTGGGAATCGGTTGAGTCAGAGAGGGATTGAGGTGAGGTTGAAGATTGTGAAGGATAGGAGAAAAGGGTGGAGCTTGTATGCTGATCAGTTCATTCCAAAGGGTCGGTTTGTCTGTGAATATGCAGGTGAACTCTTGACAACAAAAGAAGCAAGGTTGCGGCAACAAGTATATGATGAGTTTGCATCAGGTGGTAATTTTTCTCCTGCTCTTTTGGTTGTGAGGGAGCATCTTCCATCCAGAAAGGCGTGTTTAAGATACAATATCGATGCAACAAGAGTTGGAAATGTAGCAAGGTTCATCAACCATTCGTGTGACGGTGGTAACTTATCCACAACACTGATCAGAAGTTCAGGTGCTTTGCTCCCACGCCTTTGCTTTTTTGCTTCAAAAGACATACGAGAAGACGAGGAGCTTACTTTTAGCTATGGTGAAGTTACGCTAAGGTCTAAGGGTTTAAAGTGCTTTTGCGAAAGCTCTTGTTGTCTTGGAATGTTGCCGGCAGAGCAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Pre-SET SET

Protein Analysis

345

Amino Acids

37.66

Weight (kDa)

6.33

Isoelectric Point (pI)

57.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 196 - 317 4.9e-21 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 134, 635
Acc36I ACCTGC 2 cut(s) 134, 635
AccII CGCG 3 cut(s) 141, 171, 401
AciI CCGC 8 cut(s) 26, 132, 139, 166, 169, 301, 401, 679
AclI AACGTT 1 cut(s) 368
AclWI GGATC 1 cut(s) 202
AcyI GRCGYC 3 cut(s) 180, 360, 477
AfaI GTAC 2 cut(s) 242, 454
AfiI CCNNNNNNNGG 2 cut(s) 193, 310
AflIII ACRYGT 1 cut(s) 422
AgsI TTSAA 2 cut(s) 562, 909
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 2 cut(s) 192, 308
AluBI AGCT 4 cut(s) 594, 934, 945, 996
AluI AGCT 4 cut(s) 594, 934, 945, 996
Alw21I GWGCWC 1 cut(s) 71
Alw26I GTCTC 2 cut(s) 121, 413
AlwI GGATC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 3 cut(s) 143, 173, 401
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 6 cut(s) 115, 244, 362, 565, 659, 962
AvaII GGWCC 1 cut(s) 335
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 16
BccI CCATC 5 cut(s) 37, 164, 210, 271, 763
BceAI ACGGC 1 cut(s) 490
BciT130I CCWGG 2 cut(s) 194, 310
BclI TGATCA 2 cut(s) 604, 861
BcoDI GTCTC 2 cut(s) 121, 413
BfuAI ACCTGC 2 cut(s) 134, 635
BisI GCNGC 4 cut(s) 5, 169, 402, 680
BlsI GCNGC 4 cut(s) 6, 170, 403, 681
Bme1390I CCNGG 2 cut(s) 194, 310
Bme18I GGWCC 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 3 cut(s) 198, 316, 336
BmrFI CCNGG 2 cut(s) 194, 310
BmsI GCATC 3 cut(s) 713, 757, 778
BpiI GAAGAC 1 cut(s) 930
Bpu10I CCTNAGC 1 cut(s) 962
Bsa29I ATCGAT 1 cut(s) 786
BsaHI GRCGYC 3 cut(s) 180, 360, 477
BsaJI CCNNGG 2 cut(s) 86, 309
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 310
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 1 cut(s) 64
BseBI CCWGG 2 cut(s) 194, 310
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 86, 309
BseGI GGATG 4 cut(s) 29, 202, 388, 755
BseLI CCNNNNNNNGG 2 cut(s) 193, 310
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 64
BseRI GAGGAG 2 cut(s) 446, 944
BseXI GCAGC 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 3 cut(s) 141, 171, 401
Bsh1285I CGRYCG 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 786
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 318, 1019
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 193, 310
BsmAI GTCTC 2 cut(s) 121, 413
BsmBI CGTCTC 2 cut(s) 121, 413
BsmFI GGGAC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 4 cut(s) 207, 229, 604, 861
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 8 cut(s) 26, 132, 139, 166, 169, 301, 401, 679
BspCNI CTCAG 1 cut(s) 83
BspDI ATCGAT 1 cut(s) 786
BspFNI CGCG 3 cut(s) 141, 171, 401
BspLI GGNNCC 3 cut(s) 198, 316, 336
BspMI ACCTGC 2 cut(s) 134, 635
BspPI GGATC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 1018
BsrI ACTGG 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 2 cut(s) 86, 309
BssMI GATC 4 cut(s) 207, 229, 604, 861
BssNI GRCGYC 3 cut(s) 180, 360, 477
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 194, 310
Bst4CI ACNGT 2 cut(s) 115, 839
Bst6I CTCTTC 2 cut(s) 65, 289
BstACI GRCGYC 3 cut(s) 180, 360, 477
BstC8I GCNNGC 1 cut(s) 1020
BstDEI CTNAG 4 cut(s) 70, 962, 969, 1032
BstDSI CCRYGG 1 cut(s) 86
BstF5I GGATG 4 cut(s) 29, 202, 388, 755
BstFNI CGCG 3 cut(s) 141, 171, 401
BstHHI GCGC 3 cut(s) 143, 173, 401
BstKTI GATC 4 cut(s) 210, 232, 607, 864
BstMAI GTCTC 2 cut(s) 121, 413
BstMBI GATC 4 cut(s) 207, 229, 604, 861
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 4 cut(s) 13, 140, 179, 1024
BstNI CCWGG 2 cut(s) 194, 310
BstSCI CCNGG 2 cut(s) 192, 308
BstUI CGCG 3 cut(s) 141, 171, 401
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 930
Bsu15I ATCGAT 1 cut(s) 786
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 1 cut(s) 86
BtrI CACGTC 1 cut(s) 423
BtsCI GGATG 4 cut(s) 29, 202, 388, 755
BtsIMutI CAGTG 2 cut(s) 71, 857
BveI ACCTGC 2 cut(s) 134, 635
Cac8I GCNNGC 1 cut(s) 1020
CfoI GCGC 3 cut(s) 143, 173, 401
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 3 cut(s) 188, 349, 466
Csp6I GTAC 2 cut(s) 241, 453
CviAII CATG 1 cut(s) 87
CviJI RGCY 7 cut(s) 16, 91, 404, 594, 934, 945, 996
CviKI_1 RGCY 7 cut(s) 16, 91, 404, 594, 934, 945, 996
CviQI GTAC 2 cut(s) 241, 453
DdeI CTNAG 4 cut(s) 70, 962, 969, 1032
DpnI GATC 4 cut(s) 209, 231, 606, 863
DpnII GATC 4 cut(s) 207, 229, 604, 861
DraI TTTAAA 1 cut(s) 978
Eam1104I CTCTTC 2 cut(s) 65, 289
EarI CTCTTC 2 cut(s) 65, 289
EciI GGCGGA 2 cut(s) 155, 290
Eco130I CCWWGG 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 192, 308
EcoT14I CCWWGG 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 2 cut(s) 121, 413
FaeI CATG 1 cut(s) 90
FaiI YATR 8 cut(s) 88, 219, 600, 642, 691, 693, 917, 948
FaqI GGGAC 1 cut(s) 348
FatI CATG 1 cut(s) 86
FauI CCCGC 1 cut(s) 33
FbaI TGATCA 2 cut(s) 604, 861
Fnu4HI GCNGC 4 cut(s) 5, 169, 402, 680
FokI GGATG 4 cut(s) 16, 189, 395, 742
Fsp4HI GCNGC 4 cut(s) 5, 169, 402, 680
GlaI GCGC 3 cut(s) 142, 172, 400
GluI GCNGC 4 cut(s) 5, 169, 402, 680
GsaI CCCAGC 1 cut(s) 408
HapII CCGG 2 cut(s) 318, 1019
HgaI GACGC 3 cut(s) 188, 349, 466
HhaI GCGC 3 cut(s) 143, 173, 401
Hin1I GRCGYC 3 cut(s) 180, 360, 477
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 3 cut(s) 141, 171, 399
HinP1I GCGC 3 cut(s) 141, 171, 399
HinfI GANTC 3 cut(s) 386, 526, 535
HpaII CCGG 2 cut(s) 318, 1019
HphI GGTGA 6 cut(s) 115, 244, 362, 565, 659, 962
Hpy166II GTNNAC 2 cut(s) 453, 650
Hpy188I TCNGA 6 cut(s) 73, 178, 207, 487, 540, 866
Hpy188III TCNNGA 5 cut(s) 186, 288, 390, 655, 759
Hpy8I GTNNAC 2 cut(s) 453, 650
Hpy99I CGWCG 4 cut(s) 182, 230, 365, 482
HpyAV CCTTC 5 cut(s) 60, 256, 288, 431, 565
HpyCH4III ACNGT 2 cut(s) 115, 839
HpyCH4IV ACGT 2 cut(s) 368, 422
HpyCH4V TGCA 5 cut(s) 4, 129, 644, 704, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 140, 179, 1024
HpyF3I CTNAG 4 cut(s) 70, 962, 969, 1032
HpySE526I ACGT 2 cut(s) 368, 422
Hsp92I GRCGYC 3 cut(s) 180, 360, 477
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 3 cut(s) 141, 171, 399
KroI GCCGGC 1 cut(s) 1018
KroNI GCCGGC 1 cut(s) 1020
Ksp22I TGATCA 2 cut(s) 604, 861
Kzo9I GATC 4 cut(s) 207, 229, 604, 861
LmnI GCTCC 5 cut(s) 329, 591, 745, 888, 931
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 713, 757, 778
MaeII ACGT 2 cut(s) 368, 422
MaeIII GTNAC 3 cut(s) 833, 842, 955
MalI GATC 4 cut(s) 209, 231, 606, 863
MboI GATC 4 cut(s) 207, 229, 604, 861
MboII GAAGA 6 cut(s) 52, 241, 276, 574, 743, 935
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 2 cut(s) 354, 715
MlyI GAGTC 2 cut(s) 395, 544
MmeI TCCRAC 3 cut(s) 201, 495, 781
MroNI GCCGGC 1 cut(s) 1018
MseI TTAA 2 cut(s) 773, 977
MslI CAYNNNNRTG 1 cut(s) 831
MspI CCGG 2 cut(s) 318, 1019
MspR9I CCNGG 2 cut(s) 194, 310
MvaI CCWGG 2 cut(s) 194, 310
MvnI CGCG 3 cut(s) 141, 171, 401
MwoI GCNNNNNNNGC 4 cut(s) 13, 140, 179, 1024
NaeI GCCGGC 1 cut(s) 1020
NcoI CCATGG 1 cut(s) 86
NdeII GATC 4 cut(s) 207, 229, 604, 861
NgoMIV GCCGGC 1 cut(s) 1018
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 3 cut(s) 198, 316, 336
NmuCI GTSAC 1 cut(s) 833
PaqCI CACCTGC 2 cut(s) 134, 635
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PdiI GCCGGC 1 cut(s) 1020
PfeI GAWTC 1 cut(s) 526
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 476
PkrI GCNGC 4 cut(s) 6, 170, 403, 681
Ple19I CGATCG 1 cut(s) 232
PleI GAGTC 2 cut(s) 394, 543
PpsI GAGTC 2 cut(s) 394, 543
Psp1406I AACGTT 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 192, 308
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 2 cut(s) 192, 308
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 198, 316, 336
PspPI GGNCC 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 476
PvuI CGATCG 1 cut(s) 232
RsaI GTAC 2 cut(s) 242, 454
RsaNI GTAC 2 cut(s) 241, 453
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 2 cut(s) 773, 977
SatI GCNGC 4 cut(s) 5, 169, 402, 680
Sau3AI GATC 4 cut(s) 207, 229, 604, 861
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 2 cut(s) 395, 544
ScrFI CCNGG 2 cut(s) 194, 310
SduI GDGCHC 1 cut(s) 71
SfaNI GCATC 3 cut(s) 713, 757, 778
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 831
Sse9I AATT 2 cut(s) 354, 715
SsiI CCGC 8 cut(s) 26, 132, 139, 166, 169, 301, 401, 679
StyD4I CCNGG 2 cut(s) 192, 308
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 2 cut(s) 115, 839
TaiI ACGT 2 cut(s) 371, 425
TaqI TCGA 4 cut(s) 228, 232, 363, 786
TaqII GACCGA 1 cut(s) 352
TasI AATT 2 cut(s) 354, 715
TauI GCSGC 3 cut(s) 171, 404, 682
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 2 cut(s) 773, 977
Tru9I TTAA 2 cut(s) 773, 977
TscAI CASTG 2 cut(s) 71, 864
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 2 cut(s) 601, 808
TspGWI ACGGA 3 cut(s) 214, 438, 470
TspRI CASTG 2 cut(s) 71, 864
Tth111I GACNNNGTC 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.