Rw5G029000

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008003
Physical Location & Seq
Reverse (-)
44177341 .. 44179016
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029000.1

Sequence Viewer

Length: 1020 bp
ATGAACAAGAAGATGCAGTCGCCACCAACAACACCATCGAAACGCAATACCAGTCCTTCCCTTTTCTTCCGGTGCTTAGACCTCGTCCTTCCATGGCTAACCCCACAAGAGCTGGCCATGGCATCTCTGACCTGCACCACCCTCCGCAACCTCTCCAAGTCCATCACTCTCCACCGCGCCTCCGACGCTTCCAGATCCCTCGAGCCCCATCCCATCCCATTCCATAACTCACTCGATCAACACCCTTATGCCTACTTCATCTACGCCCCTTCCTCTCTAACCCCAATCATCTTCTTCTTCTTCTTCTTCACTGCTTCGCCAACCGTGGGAACTGAAACGTCGTCGTTGCGTTTTGTTGACGAATCGGGAAACTGTGCGTCGTTTGGGTGTGAATGTGAAGAGACATGTGCGGATGGGAAAGAGGGGTGTCCGTGTGTTGTCGGCTTTGCTGGCTTCGACGGCGTCGTTTTCGAGTGTGGACCCAGTTGTGGATGCCGGTTAAGTTGTGGGAATCGGTTGACTCAGACGGGGATTCAGGTTAAGTTGAAGATTGTGAGGGACAGAAGAAAAGGGTGGAGTTTGTATGCCGACCAGTTCATCCCAAAGGGTCGGTTTGTGTGCGAGTATGCAGGTGAACTATTGACAACAAAGGAATCAAGACTGCGGCAAGAAATGTATGATGAACTTTCGTCGGGTGGTCATTTCTCCCCTGCTCTTTTGGTTGTGAGGGAGCATCTTCCATCCAAAAGGGCATGTCTGAGACTCAATATTGATGCCACAAGAATCGGAAATGTCTCAAGGTTCATCAACCATTCTTGCGACGGAGGTAATTTGTCCACAGCTCTTGTTAGAAGCTCAGGGGCTTTGCTTCCACGTCTTTGCTTTTTTGCTTCAGATGACATAAAAGTGGATGAAGAGCTTACATTTAGCTATGGAGAAACTAGGCTGAACTCCAAGGGTTTGCGGTGCTTTTGTGCAAGCTCTTGCTGTGTTGGTACATTACCCTCGGAGCAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Pre-SET SET

Protein Analysis

339

Amino Acids

37.4

Weight (kDa)

8.03

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 191 - 312 1.1e-19 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 620
Acc36I ACCTGC 2 cut(s) 140, 620
AccII CGCG 1 cut(s) 177
AciI CCGC 5 cut(s) 145, 175, 410, 664, 964
AclWI GGATC 1 cut(s) 189
AcoI YGGCCR 1 cut(s) 114
AcuI CTGAAG 1 cut(s) 876
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 1 cut(s) 997
AfiI CCNNNNNNNGG 2 cut(s) 326, 488
AflIII ACRYGT 1 cut(s) 404
AgsI TTSAA 1 cut(s) 547
AjiI CACGTC 1 cut(s) 875
AluBI AGCT 6 cut(s) 112, 842, 855, 919, 930, 981
AluI AGCT 6 cut(s) 112, 842, 855, 919, 930, 981
Alw26I GTCTC 3 cut(s) 395, 754, 799
AlwI GGATC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 1 cut(s) 114
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 644
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 479
BalI TGGCCA 1 cut(s) 116
BanII GRGCYC 1 cut(s) 207
BccI CCATC 6 cut(s) 43, 170, 216, 221, 407, 748
BceAI ACGGC 1 cut(s) 475
BcoDI GTCTC 3 cut(s) 395, 754, 799
BfaI CTAG 1 cut(s) 942
BfuAI ACCTGC 2 cut(s) 140, 620
BisI GCNGC 1 cut(s) 665
BlsI GCNGC 1 cut(s) 666
Bme18I GGWCC 1 cut(s) 479
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 875
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 481
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 5 cut(s) 3, 131, 482, 742, 763
BmuI ACTGGG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 856
BpuEI CTTGAG 1 cut(s) 781
BsaHI GRCGYC 1 cut(s) 462
BsaJI CCNNGG 5 cut(s) 92, 117, 324, 954, 1005
BsaWI WCCGGW 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 488
Bse118I RCCGGY 1 cut(s) 495
Bse1I ACTGG 3 cut(s) 51, 483, 592
BseDI CCNNGG 5 cut(s) 92, 117, 324, 954, 1005
BseGI GGATG 7 cut(s) 208, 213, 418, 497, 597, 740, 916
BseLI CCNNNNNNNGG 2 cut(s) 326, 488
BseMII CTCAG 3 cut(s) 536, 749, 870
BseNI ACTGG 3 cut(s) 51, 483, 592
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 70, 496
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 2 cut(s) 326, 488
BsmAI GTCTC 3 cut(s) 395, 754, 799
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 194, 235
Bsp19I CCATGG 2 cut(s) 92, 117
BspACI CCGC 5 cut(s) 145, 175, 410, 664, 964
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 3 cut(s) 535, 750, 869
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 481
BspMI ACCTGC 2 cut(s) 140, 620
BspPI GGATC 1 cut(s) 189
BspQI GCTCTTC 1 cut(s) 909
BsrFI RCCGGY 1 cut(s) 495
BsrI ACTGG 3 cut(s) 51, 483, 592
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 5 cut(s) 92, 117, 324, 954, 1005
BssMI GATC 2 cut(s) 194, 235
BssNI GRCGYC 1 cut(s) 462
BssT1I CCWWGG 3 cut(s) 92, 117, 954
Bst4CI ACNGT 2 cut(s) 325, 374
Bst6I CTCTTC 2 cut(s) 393, 909
BstACI GRCGYC 1 cut(s) 462
BstC8I GCNNGC 3 cut(s) 114, 451, 979
BstDEI CTNAG 4 cut(s) 76, 522, 758, 856
BstDSI CCRYGG 3 cut(s) 92, 117, 324
BstF5I GGATG 7 cut(s) 208, 213, 418, 497, 597, 740, 916
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 2 cut(s) 197, 238
BstMAI GTCTC 3 cut(s) 395, 754, 799
BstMBI GATC 2 cut(s) 194, 235
BstMWI GCNNNNNNNGC 3 cut(s) 185, 450, 459
BstNSI RCATGY 2 cut(s) 408, 756
BstUI CGCG 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 3 cut(s) 92, 117, 324
BtrI CACGTC 1 cut(s) 875
BtsCI GGATG 7 cut(s) 208, 213, 418, 497, 597, 740, 916
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
BveI ACCTGC 2 cut(s) 140, 620
Cac8I GCNNGC 3 cut(s) 114, 451, 979
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 1 cut(s) 479
CseI GACGC 3 cut(s) 194, 366, 451
Csp6I GTAC 1 cut(s) 996
CspCI CAANNNNNGTGG 2 cut(s) 826, 861
CviAII CATG 4 cut(s) 93, 118, 405, 753
CviQI GTAC 1 cut(s) 996
DdeI CTNAG 4 cut(s) 76, 522, 758, 856
DpnI GATC 2 cut(s) 196, 237
DpnII GATC 2 cut(s) 194, 235
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 2 cut(s) 393, 909
EarI CTCTTC 2 cut(s) 393, 909
Eco130I CCWWGG 3 cut(s) 92, 117, 954
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 200
EcoT14I CCWWGG 3 cut(s) 92, 117, 954
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 3 cut(s) 92, 117, 954
FaeI CATG 4 cut(s) 96, 121, 408, 756
FaqI GGGAC 1 cut(s) 572
FatI CATG 4 cut(s) 92, 117, 404, 752
Fnu4HI GCNGC 1 cut(s) 665
FokI GGATG 7 cut(s) 195, 200, 425, 504, 584, 727, 923
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 665
FspBI CTAG 1 cut(s) 942
GlaI GCGC 1 cut(s) 178
GluI GCNGC 1 cut(s) 665
HaeIII GGCC 1 cut(s) 116
HapII CCGG 2 cut(s) 70, 496
HgaI GACGC 3 cut(s) 194, 366, 451
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 4 cut(s) 96, 121, 408, 756
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 2 cut(s) 358, 519
HindII GTYRAC 2 cut(s) 358, 519
HinfI GANTC 7 cut(s) 362, 511, 520, 532, 653, 762, 783
HpaII CCGG 2 cut(s) 70, 496
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 5 cut(s) 358, 479, 519, 635, 837
Hpy188I TCNGA 7 cut(s) 129, 184, 525, 759, 788, 895, 1009
Hpy188III TCNNGA 3 cut(s) 192, 366, 657
Hpy8I GTNNAC 5 cut(s) 358, 479, 519, 635, 837
Hpy99I CGWCG 8 cut(s) 188, 343, 346, 382, 461, 467, 694, 824
HpyAV CCTTC 3 cut(s) 66, 98, 279
HpyCH4III ACNGT 2 cut(s) 325, 374
HpyCH4IV ACGT 2 cut(s) 338, 874
HpyCH4V TGCA 4 cut(s) 16, 135, 629, 977
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 450, 459
HpyF3I CTNAG 4 cut(s) 76, 522, 758, 856
HpySE526I ACGT 2 cut(s) 338, 874
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 4 cut(s) 96, 121, 408, 756
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 2 cut(s) 194, 235
LguI GCTCTTC 1 cut(s) 909
LmnI GCTCC 2 cut(s) 730, 1009
LweI GCATC 5 cut(s) 3, 131, 482, 742, 763
MaeI CTAG 1 cut(s) 942
MaeII ACGT 2 cut(s) 338, 874
MalI GATC 2 cut(s) 196, 237
MboI GATC 2 cut(s) 194, 235
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 1 cut(s) 829
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 2 cut(s) 514, 756
MmeI TCCRAC 1 cut(s) 207
Mox20I TGGCCA 1 cut(s) 116
MscI TGGCCA 1 cut(s) 116
MseI TTAA 3 cut(s) 500, 540, 1018
MslI CAYNNNNRTG 2 cut(s) 246, 905
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 2 cut(s) 70, 496
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 185, 450, 459
NcoI CCATGG 2 cut(s) 92, 117
NdeII GATC 2 cut(s) 194, 235
NlaIII CATG 4 cut(s) 96, 121, 408, 756
NlaIV GGNNCC 1 cut(s) 481
NspI RCATGY 2 cut(s) 408, 756
PaeR7I CTCGAG 1 cut(s) 200
PaqCI CACCTGC 1 cut(s) 620
PciI ACATGT 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 909
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PfeI GAWTC 5 cut(s) 362, 511, 532, 653, 783
PflFI GACNNNGTC 2 cut(s) 83, 461
PkrI GCNGC 1 cut(s) 666
PleI GAGTC 2 cut(s) 514, 756
PpsI GAGTC 2 cut(s) 514, 756
PscI ACATGT 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 1 cut(s) 479
PspXI VCTCGAGB 1 cut(s) 200
PsuI RGATCY 1 cut(s) 194
PsyI GACNNNGTC 2 cut(s) 83, 461
RsaI GTAC 1 cut(s) 997
RsaNI GTAC 1 cut(s) 996
RseI CAYNNNNRTG 2 cut(s) 246, 905
SapI GCTCTTC 1 cut(s) 909
SaqAI TTAA 3 cut(s) 500, 540, 1018
SatI GCNGC 1 cut(s) 665
Sau3AI GATC 2 cut(s) 194, 235
Sau96I GGNCC 1 cut(s) 479
SchI GAGTC 2 cut(s) 514, 756
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 5 cut(s) 3, 131, 482, 742, 763
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 2 cut(s) 246, 905
SmlI CTYRAG 2 cut(s) 200, 796
SmoI CTYRAG 2 cut(s) 200, 796
Sse9I AATT 1 cut(s) 829
SsiI CCGC 5 cut(s) 145, 175, 410, 664, 964
SspI AATATT 1 cut(s) 769
SspMI CTAG 1 cut(s) 942
StyI CCWWGG 3 cut(s) 92, 117, 954
TaaI ACNGT 2 cut(s) 325, 374
TaiI ACGT 2 cut(s) 341, 877
TaqI TCGA 5 cut(s) 38, 201, 234, 456, 471
TasI AATT 1 cut(s) 829
TauI GCSGC 1 cut(s) 667
TfiI GAWTC 5 cut(s) 362, 511, 532, 653, 783
Tru1I TTAA 3 cut(s) 500, 540, 1018
Tru9I TTAA 3 cut(s) 500, 540, 1018
TscAI CASTG 1 cut(s) 316
TspDTI ATGAA 6 cut(s) 17, 247, 586, 696, 793, 927
TspGWI ACGGA 2 cut(s) 420, 837
TspRI CASTG 1 cut(s) 316
Tth111I GACNNNGTC 2 cut(s) 83, 461
VpaK11BI GGWCC 1 cut(s) 479
XceI RCATGY 2 cut(s) 408, 756
XhoI CTCGAG 1 cut(s) 200
XspI CTAG 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.