Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006498

Physical Location & Seq

Genomic Coordinates Reverse (-)

2224739 .. 2227957

Gene Structure 3219 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1022500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGAGGATGTTACAAAATCTACTGGTGATTCTTGTATTTAGTACTTATTTAGTAGCCACGTTGGGTGAAGGAATACGTGAGGATGGCGACGATGGTAGGAAGCCTTACATTGTGTACATGGGAGAACTGCCAGCACCAGAAATTTCCGTCATGAGCAGACACCATAACCTGCTCGCTCAAGCCATCGGAGATGTCAAAGTAGCCAGAGAATCAAGAATTCATAGTTATGGAAGGAGTTTCAATGGGTTCGTAGCAAGATTGTTGCCACATGAAGCACAGGCACTCTCAGATGATGATAGCGTTGTGTCAGTGTTCCCAAACACCGTGCGCAAACTTCATACAACAAGGTCATGGGATTACCTAGGGTTGCCTTTGAAATCAAATAAAAGGAACTCTCATATAGAAAGCAATACCATAGTGGGTGTGTTGGATTCAGGAATTTACATGCAGGCTCCCAGTTTCAACGATAAAGGCTACGGACCACCCCCAGCTAAATGGAAAGGCAAATGTATGAAGGGTGCAAACTTCACAGGCTGCAACAACAAGGTAATTGGTGCCAAATATTTTAAACTGAACATTGCCCGTACTTGGGGCATTCAAAGTCCAGTCGACGATGATGGCCACGGCACTCACACTGCCTCCACCATTGCTGGTGCAGCCGTCAAAGGTGCCAGCTTATATGGCGTTGCAAAAGGGACTGCACGCGGGGGCGTGCCATTGTCACGCATCGCTGTGTACAAAGTGTGCTGGAGCACTCTTGGTTGCAGTGACATGGATATTTTGGCCGCATTTGACGAGGCCATTGCCGACGGTGTGGACTTAATATCGATCTCAATTGGTGGTCACTCGCGGAGGTTTTTGGACGATCCCATTGCCATCGGATCATTTCATGCCATGAAAAAAGGGATTTTTGTGTCGTGCTCAGCTGGGAATGATGGGCTTCCGGCCAGTGTGCAGAATGTAGCACCATGGATTATGACAGTGGCTGCTAATACCCTTGATAGGCAGTTACAGACTGTAGTTAAACTTGGCAATGGCCAGAGGATTTCTGGAAATGCACTCAATACCTATTCGCCGAAGAAACTGATGTATCCTCTTATAAGTGGAGCCCTTGCATATAACACGAGTAATGAATTCGGGGGAAATGCAAGTGCTTGTGATTCTTGGACTCTAAACGCTAACCTGGTGAAGGGGAGGATTGTGTACTGCCAAGGTTTTGGTTATCAGGATTACACTATTCAACGGTTAAAGGGTGCTGGAACCCTAATGACACGTGAAGAACTTAATGATTATGCCTATAGCCCTATAATCCCTGGTACCGATATCCTTGTCCAAGATGGGATCAAGATCGATAAGTACATCAACTCTACCAAAAACCCTACGGCAGTCATATACAAGACCAGAACTCCTAGTACGACCGCCCCCATCGTCGCTTCTTTCTCATCGAGAGGACCTCAACAGATAACCCTCAACATCCTCAAGCCTGATATTTCTGCACCAGGGGTAAATATATTGGCTGCATATTCAAAGATACCATCCATAACTGGTTACCCAGAAGATCGGCGCCACAATGTGTTCAATGTGTTATCAGGTACGTCCATGTCTTGCCCTCATGTAGCAGCAGCAGCAGCCTATGTCAAGTCTTTCCACCCGGACTGGTCACCTGCTGCCATCAAGTCCGCTCTCATGACAACTGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

567

Amino Acids

60.89

Weight (kDa)

9.18

Isoelectric Point (pI)

35.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	36 - 113	1.2e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	136 - 565	2.1e-42	Subtilase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006498)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03620 AT5G03620
malus_domestica	MD12G1022400.v1.1 MD12G1022500.v1.1 MD14G1019300.v1.1 MD14G1019400.v1.1
prunus_persica	Prupe.7G114300_v2.0.a1 Prupe.7G114300_v2.0.a1
pyrus_communis	pycom14g01900
rosa_chinensis	RchiOBHm_Chr3g0472671 RchiOBHm_Chr3g0472681
rosa_laevigata	RLG00000016130 RLG00000024064
rosa_multiflora	Rmu_sc0001616.1_g000054 Rmu_sc0003510.1_g000013
rosa_roxburghii	Rroxscaffold_5G00353850 Rroxscaffold_6G00408720 Rroxscaffold_6G00408730
rosa_rugosa	Rorug03G0127100 Rorug04G0092600
rosa_samantha	Rh3AG179800 Rh3BG205700 Rh3CG202800 Rh3DG170000 Rh3DG170100
rosa_wichuraiana	Rw3G016290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1100
AarI	CACCTGC	1 cut(s)	1672
Acc16I	TGCGCA	1 cut(s)	329
Acc36I	ACCTGC	2 cut(s)	177, 1672
Acc65I	GGTACC	1 cut(s)	1316
AccB1I	GGYRCC	4 cut(s)	554, 668, 1316, 1563
AccBSI	CCGCTC	1 cut(s)	1682
AccI	GTMKAC	1 cut(s)	609
AccII	CGCG	2 cut(s)	705, 850
AciI	CCGC	5 cut(s)	705, 786, 850, 1419, 1680
AclWI	GGATC	3 cut(s)	860, 889, 1349
AcoI	YGGCCR	4 cut(s)	619, 783, 945, 1036
AcsI	RAATTY	4 cut(s)	141, 216, 438, 1133
AcvI	CACGTG	1 cut(s)	1274
AcyI	GRCGYC	1 cut(s)	1564
AdeI	CACNNNGTG	1 cut(s)	1573
AfaI	GTAC	9 cut(s)	43, 116, 586, 737, 1205, 1318, 1358, 1414, 1594
AfiI	CCNNNNNNNGG	4 cut(s)	588, 589, 1002, 1189
AflIII	ACRYGT	1 cut(s)	1271
AgsI	TTSAA	7 cut(s)	241, 376, 463, 599, 1241, 1527, 1579
AjnI	CCWGG	3 cut(s)	1182, 1312, 1498
AluBI	AGCT	3 cut(s)	491, 675, 926
AluI	AGCT	3 cut(s)	491, 675, 926
Alw21I	GWGCWC	2 cut(s)	755, 923
AlwI	GGATC	3 cut(s)	860, 889, 1349
AlwNI	CAGNNNCTG	1 cut(s)	986
AoxI	GGCC	5 cut(s)	619, 783, 798, 945, 1036
ApeKI	GCWGC	9 cut(s)	534, 656, 986, 1517, 1619, 1622, 1625, 1628, 1667
ApoI	RAATTY	4 cut(s)	141, 216, 438, 1133
Asp718I	GGTACC	1 cut(s)	1316
AspA2I	CCTAGG	1 cut(s)	361
AspLEI	GCGC	2 cut(s)	330, 1566
AspS9I	GGNCC	2 cut(s)	479, 1451
AsuC2I	CCSGG	1 cut(s)	1652
AsuHPI	GGTGA	4 cut(s)	37, 77, 1198, 1653
AvaII	GGWCC	2 cut(s)	479, 1451
AvrII	CCTAGG	1 cut(s)	361
BaeI	ACNNNNGTAYC	2 cut(s)	1073, 1106
BalI	TGGCCA	2 cut(s)	621, 1038
BanI	GGYRCC	4 cut(s)	554, 668, 1316, 1563
BanII	GRGCYC	1 cut(s)	1111
BauI	CACGAG	1 cut(s)	1123
BbrPI	CACGTG	1 cut(s)	1274
Bbv12I	GWGCWC	2 cut(s)	755, 923
BbvI	GCAGC	9 cut(s)	521, 668, 973, 1504, 1631, 1634, 1637, 1640, 1654
BceAI	ACGGC	3 cut(s)	640, 644, 1398
BcgI	CGANNNNNNTGC	4 cut(s)	785, 819, 854, 888
BciT130I	CCWGG	3 cut(s)	1184, 1314, 1500
BciVI	GTATCC	1 cut(s)	1101
BcnI	CCSGG	1 cut(s)	1652
BfaI	CTAG	2 cut(s)	362, 1410
BfmI	CTRYAG	2 cut(s)	1017, 1297
BfoI	RGCGCY	1 cut(s)	1567
BfuAI	ACCTGC	2 cut(s)	177, 1672
BfuI	GTATCC	1 cut(s)	1101
BlnI	CCTAGG	1 cut(s)	361
BlpI	GCTNAGC	1 cut(s)	922
BmcAI	AGTACT	1 cut(s)	43
Bme1390I	CCNGG	4 cut(s)	1184, 1314, 1500, 1652
Bme18I	GGWCC	2 cut(s)	479, 1451
BmgT120I	GGNCC	2 cut(s)	479, 1451
BmiI	GGNNCC	7 cut(s)	453, 556, 670, 1108, 1261, 1318, 1565
BmrFI	CCNGG	4 cut(s)	1184, 1314, 1500, 1652
BmrI	ACTGGG	1 cut(s)	450
BmsI	GCATC	1 cut(s)	735
BmuI	ACTGGG	1 cut(s)	450
BplI	GAGNNNNNCTC	2 cut(s)	1438, 1470
BpmI	CTGGAG	1 cut(s)	769
Bpu1102I	GCTNAGC	1 cut(s)	922
BpuEI	CTTGAG	2 cut(s)	162, 1463
BpuMI	CCSGG	1 cut(s)	1652
Bsa29I	ATCGAT	2 cut(s)	827, 1350
BsaAI	YACGTR	2 cut(s)	77, 1274
BsaBI	GATNNNNATC	1 cut(s)	1346
BsaHI	GRCGYC	1 cut(s)	1564
BsaJI	CCNNGG	6 cut(s)	361, 622, 968, 1210, 1312, 1499
BsaXI	ACNNNNNCTCC	4 cut(s)	623, 653, 1390, 1420
Bsc4I	CCNNNNNNNGG	4 cut(s)	588, 589, 1002, 1189
Bse1I	ACTGG	7 cut(s)	27, 456, 605, 948, 1549, 1661, 1699
Bse3DI	GCAATG	5 cut(s)	576, 645, 801, 870, 1039
Bse8I	GATNNNNATC	1 cut(s)	1346
BseBI	CCWGG	3 cut(s)	1184, 1314, 1500
BseCI	ATCGAT	2 cut(s)	827, 1350
BseDI	CCNNGG	6 cut(s)	361, 622, 968, 1210, 1312, 1499
BseGI	GGATG	4 cut(s)	12, 88, 1473, 1535
BseJI	GATNNNNATC	1 cut(s)	1346
BseLI	CCNNNNNNNGG	4 cut(s)	588, 589, 1002, 1189
BseMI	GCAATG	5 cut(s)	576, 645, 801, 870, 1039
BseMII	CTCAG	2 cut(s)	300, 936
BseNI	ACTGG	7 cut(s)	27, 456, 605, 948, 1549, 1661, 1699
BseXI	GCAGC	9 cut(s)	521, 668, 973, 1504, 1631, 1634, 1637, 1640, 1654
BseYI	CCCAGC	2 cut(s)	487, 926
BsgI	GTGCAG	4 cut(s)	675, 684, 974, 1479
Bsh1236I	CGCG	2 cut(s)	705, 850
Bsh1285I	CGRYCG	1 cut(s)	1419
BshFI	GGCC	5 cut(s)	621, 785, 800, 947, 1038
BshNI	GGYRCC	4 cut(s)	554, 668, 1316, 1563
BshVI	ATCGAT	2 cut(s)	827, 1350
BsiEI	CGRYCG	1 cut(s)	1419
BsiHKAI	GWGCWC	2 cut(s)	755, 923
BsiSI	CCGG	2 cut(s)	944, 1652
BslFI	GGGAC	1 cut(s)	709
BslI	CCNNNNNNNGG	4 cut(s)	588, 589, 1002, 1189
BsmFI	GGGAC	1 cut(s)	709
BsmI	GAATGC	1 cut(s)	594
BsnI	GGCC	5 cut(s)	621, 785, 800, 947, 1038
Bsp1286I	GDGCHC	3 cut(s)	755, 923, 1111
Bsp1407I	TGTACA	2 cut(s)	114, 735
Bsp143I	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
Bsp1720I	GCTNAGC	1 cut(s)	922
Bsp19I	CCATGG	1 cut(s)	968
BspACI	CCGC	5 cut(s)	705, 786, 850, 1419, 1680
BspANI	GGCC	5 cut(s)	621, 785, 800, 947, 1038
BspCNI	CTCAG	2 cut(s)	299, 935
BspDI	ATCGAT	2 cut(s)	827, 1350
BspFNI	CGCG	2 cut(s)	705, 850
BspHI	TCATGA	2 cut(s)	150, 1686
BspLI	GGNNCC	7 cut(s)	453, 556, 670, 1108, 1261, 1318, 1565
BspMI	ACCTGC	2 cut(s)	177, 1672
BspPI	GGATC	3 cut(s)	860, 889, 1349
BspT107I	GGYRCC	4 cut(s)	554, 668, 1316, 1563
BsrBI	CCGCTC	1 cut(s)	1682
BsrDI	GCAATG	5 cut(s)	576, 645, 801, 870, 1039
BsrGI	TGTACA	2 cut(s)	114, 735
BsrI	ACTGG	7 cut(s)	27, 456, 605, 948, 1549, 1661, 1699
BssECI	CCNNGG	6 cut(s)	361, 622, 968, 1210, 1312, 1499
BssMI	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
BssNI	GRCGYC	1 cut(s)	1564
BssSI	CACGAG	1 cut(s)	1123
BssT1I	CCWWGG	3 cut(s)	361, 968, 1210
Bst2BI	CACGAG	1 cut(s)	1123
Bst2UI	CCWGG	3 cut(s)	1184, 1314, 1500
Bst4CI	ACNGT	5 cut(s)	325, 812, 982, 1018, 1245
BstACI	GRCGYC	1 cut(s)	1564
BstAUI	TGTACA	2 cut(s)	114, 735
BstBAI	YACGTR	2 cut(s)	77, 1274
BstC8I	GCNNGC	6 cut(s)	132, 174, 450, 673, 703, 713
BstDEI	CTNAG	2 cut(s)	286, 922
BstDSI	CCRYGG	2 cut(s)	622, 968
BstEII	GGTNACC	2 cut(s)	1547, 1659
BstENI	CCTNNNNNAGG	1 cut(s)	1187
BstF5I	GGATG	4 cut(s)	12, 88, 1473, 1535
BstFNI	CGCG	2 cut(s)	705, 850
BstH2I	RGCGCY	1 cut(s)	1567
BstHHI	GCGC	2 cut(s)	330, 1566
BstKTI	GATC	6 cut(s)	831, 868, 884, 1344, 1350, 1561
BstMBI	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
BstMCI	CGRYCG	1 cut(s)	1419
BstMWI	GCNNNNNNNGC	4 cut(s)	656, 681, 1625, 1628
BstNI	CCWGG	3 cut(s)	1184, 1314, 1500
BstNSI	RCATGY	1 cut(s)	448
BstPI	GGTNACC	2 cut(s)	1547, 1659
BstSCI	CCNGG	4 cut(s)	1182, 1312, 1498, 1650
BstSFI	CTRYAG	2 cut(s)	1017, 1297
BstUI	CGCG	2 cut(s)	705, 850
BstV1I	GCAGC	9 cut(s)	521, 668, 973, 1504, 1631, 1634, 1637, 1640, 1654
BstXI	CCANNNNNNTGG	2 cut(s)	495, 1217
Bsu15I	ATCGAT	2 cut(s)	827, 1350
BsuI	GTATCC	1 cut(s)	1101
BsuRI	GGCC	5 cut(s)	621, 785, 800, 947, 1038
BsuTUI	ATCGAT	2 cut(s)	827, 1350
BtgI	CCRYGG	2 cut(s)	622, 968
BtgZI	GCGATG	1 cut(s)	712
BtsCI	GGATG	4 cut(s)	12, 88, 1473, 1535
BtsI	GCAGTG	2 cut(s)	633, 772
BtsIMutI	CAGTG	5 cut(s)	315, 633, 772, 955, 987
BveI	ACCTGC	2 cut(s)	177, 1672
Cac8I	GCNNGC	6 cut(s)	132, 174, 450, 673, 703, 713
CaiI	CAGNNNCTG	1 cut(s)	986
CciI	TCATGA	2 cut(s)	150, 1686
CfoI	GCGC	2 cut(s)	330, 1566
Cfr13I	GGNCC	2 cut(s)	479, 1451
ClaI	ATCGAT	2 cut(s)	827, 1350
CsiI	ACCWGGT	1 cut(s)	1182
Csp6I	GTAC	9 cut(s)	42, 115, 585, 736, 1204, 1317, 1357, 1413, 1593
CviQI	GTAC	9 cut(s)	42, 115, 585, 736, 1204, 1317, 1357, 1413, 1593
DdeI	CTNAG	2 cut(s)	286, 922
DinI	GGCGCC	1 cut(s)	1565
DpnI	GATC	6 cut(s)	830, 867, 883, 1343, 1349, 1560
DpnII	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
DraI	TTTAAA	1 cut(s)	568
DraIII	CACNNNGTG	1 cut(s)	1573
EaeI	YGGCCR	4 cut(s)	619, 783, 945, 1036
Eco130I	CCWWGG	3 cut(s)	361, 968, 1210
Eco24I	GRGCYC	1 cut(s)	1111
Eco32I	GATATC	1 cut(s)	1324
Eco47I	GGWCC	2 cut(s)	479, 1451
Eco72I	CACGTG	1 cut(s)	1274
Eco91I	GGTNACC	2 cut(s)	1547, 1659
EcoNI	CCTNNNNNAGG	1 cut(s)	1187
EcoO109I	RGGNCCY	1 cut(s)	1451
EcoO65I	GGTNACC	2 cut(s)	1547, 1659
EcoRI	GAATTC	2 cut(s)	216, 1133
EcoRII	CCWGG	3 cut(s)	1182, 1312, 1498
EcoRV	GATATC	1 cut(s)	1324
EcoT14I	CCWWGG	3 cut(s)	361, 968, 1210
EcoT38I	GRGCYC	1 cut(s)	1111
EgeI	GGCGCC	1 cut(s)	1565
EheI	GGCGCC	1 cut(s)	1565
ErhI	CCWWGG	3 cut(s)	361, 968, 1210
FaqI	GGGAC	1 cut(s)	709
FauI	CCCGC	1 cut(s)	698
FblI	GTMKAC	1 cut(s)	609
FokI	GGATG	4 cut(s)	19, 95, 1460, 1522
FriOI	GRGCYC	1 cut(s)	1111
FspBI	CTAG	2 cut(s)	362, 1410
FspI	TGCGCA	1 cut(s)	329
GlaI	GCGC	2 cut(s)	329, 1565
GsaI	CCCAGC	2 cut(s)	491, 930
GsuI	CTGGAG	1 cut(s)	769
HaeII	RGCGCY	1 cut(s)	1567
HaeIII	GGCC	5 cut(s)	621, 785, 800, 947, 1038
HapII	CCGG	2 cut(s)	944, 1652
HhaI	GCGC	2 cut(s)	330, 1566
Hin1I	GRCGYC	1 cut(s)	1564
Hin6I	GCGC	2 cut(s)	328, 1564
HinP1I	GCGC	2 cut(s)	328, 1564
HincII	GTYRAC	1 cut(s)	610
HindII	GTYRAC	1 cut(s)	610
HinfI	GANTC	5 cut(s)	28, 209, 431, 1160, 1168
HpaII	CCGG	2 cut(s)	944, 1652
HphI	GGTGA	4 cut(s)	37, 77, 1198, 1653
Hpy166II	GTNNAC	5 cut(s)	115, 610, 736, 817, 1204
Hpy188I	TCNGA	3 cut(s)	188, 289, 881
Hpy188III	TCNNGA	8 cut(s)	151, 213, 435, 1050, 1226, 1345, 1446, 1687
Hpy8I	GTNNAC	5 cut(s)	115, 610, 736, 817, 1204
Hpy99I	CGWCG	4 cut(s)	92, 614, 812, 1433
HpyAV	CCTTC	4 cut(s)	62, 225, 508, 1183
HpyCH4III	ACNGT	5 cut(s)	325, 812, 982, 1018, 1245
HpyCH4IV	ACGT	4 cut(s)	59, 76, 1273, 1595
HpyF10VI	GCNNNNNNNGC	4 cut(s)	656, 681, 1625, 1628
HpyF3I	CTNAG	2 cut(s)	286, 922
HpySE526I	ACGT	4 cut(s)	59, 76, 1273, 1595
Hsp92I	GRCGYC	1 cut(s)	1564
HspAI	GCGC	2 cut(s)	328, 1564
KasI	GGCGCC	1 cut(s)	1563
KpnI	GGTACC	1 cut(s)	1320
Kzo9I	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
LmnI	GCTCC	3 cut(s)	457, 750, 1106
Lsp1109I	GCAGC	9 cut(s)	521, 668, 973, 1504, 1631, 1634, 1637, 1640, 1654
LweI	GCATC	1 cut(s)	735
MabI	ACCWGGT	1 cut(s)	1182
MaeI	CTAG	2 cut(s)	362, 1410
MaeII	ACGT	4 cut(s)	59, 76, 1273, 1595
MaeIII	GTNAC	7 cut(s)	9, 720, 767, 842, 1008, 1547, 1659
MalI	GATC	6 cut(s)	830, 867, 883, 1343, 1349, 1560
MbiI	CCGCTC	1 cut(s)	1682
MboI	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
MboII	GAAGA	3 cut(s)	1090, 1289, 1568
MfeI	CAATTG	1 cut(s)	834
MhlI	GDGCHC	3 cut(s)	755, 923, 1111
MlsI	TGGCCA	2 cut(s)	621, 1038
MluCI	AATT	6 cut(s)	141, 216, 438, 549, 834, 1133
MluNI	TGGCCA	2 cut(s)	621, 1038
Mly113I	GGCGCC	1 cut(s)	1564
MlyI	GAGTC	1 cut(s)	1162
MmeI	TCCRAC	1 cut(s)	408
Mox20I	TGGCCA	2 cut(s)	621, 1038
MscI	TGGCCA	2 cut(s)	621, 1038
MseI	TTAA	5 cut(s)	567, 821, 1023, 1247, 1284
MslI	CAYNNNNRTG	2 cut(s)	225, 731
Msp20I	TGGCCA	2 cut(s)	621, 1038
MspA1I	CMGCKG	1 cut(s)	926
MspI	CCGG	2 cut(s)	944, 1652
MspR9I	CCNGG	4 cut(s)	1184, 1314, 1500, 1652
MunI	CAATTG	1 cut(s)	834
Mva1269I	GAATGC	1 cut(s)	594
MvaI	CCWGG	3 cut(s)	1184, 1314, 1500
MvnI	CGCG	2 cut(s)	705, 850
MwoI	GCNNNNNNNGC	4 cut(s)	656, 681, 1625, 1628
NarI	GGCGCC	1 cut(s)	1564
NciI	CCSGG	1 cut(s)	1652
NcoI	CCATGG	1 cut(s)	968
NdeII	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
NlaIV	GGNNCC	7 cut(s)	453, 556, 670, 1108, 1261, 1318, 1565
NmuCI	GTSAC	4 cut(s)	720, 767, 842, 1659
NsbI	TGCGCA	1 cut(s)	329
NspI	RCATGY	1 cut(s)	448
PagI	TCATGA	2 cut(s)	150, 1686
PaqCI	CACCTGC	1 cut(s)	1672
PctI	GAATGC	1 cut(s)	594
PfeI	GAWTC	4 cut(s)	28, 209, 431, 1160
PleI	GAGTC	1 cut(s)	1162
PluTI	GGCGCC	1 cut(s)	1567
PmaCI	CACGTG	1 cut(s)	1274
PmlI	CACGTG	1 cut(s)	1274
PpsI	GAGTC	1 cut(s)	1162
Ppu21I	YACGTR	2 cut(s)	77, 1274
PpuMI	RGGWCCY	1 cut(s)	1451
PsiI	TTATAA	1 cut(s)	1100
Psp5II	RGGWCCY	1 cut(s)	1451
Psp6I	CCWGG	3 cut(s)	1182, 1312, 1498
PspCI	CACGTG	1 cut(s)	1274
PspEI	GGTNACC	2 cut(s)	1547, 1659
PspFI	CCCAGC	2 cut(s)	487, 926
PspGI	CCWGG	3 cut(s)	1182, 1312, 1498
PspN4I	GGNNCC	7 cut(s)	453, 556, 670, 1108, 1261, 1318, 1565
PspPI	GGNCC	2 cut(s)	479, 1451
PspPPI	RGGWCCY	1 cut(s)	1451
PsrI	GAACNNNNNNTAC	2 cut(s)	1396, 1428
PstNI	CAGNNNCTG	1 cut(s)	986
PvuII	CAGCTG	1 cut(s)	926
RsaI	GTAC	9 cut(s)	43, 116, 586, 737, 1205, 1318, 1358, 1414, 1594
RsaNI	GTAC	9 cut(s)	42, 115, 585, 736, 1204, 1317, 1357, 1413, 1593
RseI	CAYNNNNRTG	2 cut(s)	225, 731
SalI	GTCGAC	1 cut(s)	608
SaqAI	TTAA	5 cut(s)	567, 821, 1023, 1247, 1284
Sau3AI	GATC	6 cut(s)	828, 865, 881, 1341, 1347, 1558
Sau96I	GGNCC	2 cut(s)	479, 1451
ScaI	AGTACT	1 cut(s)	43
SchI	GAGTC	1 cut(s)	1162
ScrFI	CCNGG	4 cut(s)	1184, 1314, 1500, 1652
SduI	GDGCHC	3 cut(s)	755, 923, 1111
SexAI	ACCWGGT	1 cut(s)	1182
SfaNI	GCATC	1 cut(s)	735
SfcI	CTRYAG	2 cut(s)	1017, 1297
SfoI	GGCGCC	1 cut(s)	1565
SinI	GGWCC	2 cut(s)	479, 1451
SmiMI	CAYNNNNRTG	2 cut(s)	225, 731
SmlI	CTYRAG	2 cut(s)	177, 1478
SmoI	CTYRAG	2 cut(s)	177, 1478
Sse9I	AATT	6 cut(s)	141, 216, 438, 549, 834, 1133
SsiI	CCGC	5 cut(s)	705, 786, 850, 1419, 1680
SspDI	GGCGCC	1 cut(s)	1563
SspI	AATATT	1 cut(s)	563
SspMI	CTAG	2 cut(s)	362, 1410
StyD4I	CCNGG	4 cut(s)	1182, 1312, 1498, 1650
StyI	CCWWGG	3 cut(s)	361, 968, 1210
TaaI	ACNGT	5 cut(s)	325, 812, 982, 1018, 1245
TaiI	ACGT	4 cut(s)	62, 79, 1276, 1598
TaqI	TCGA	4 cut(s)	609, 827, 1350, 1445
TasI	AATT	6 cut(s)	141, 216, 438, 549, 834, 1133
TatI	WGTACW	5 cut(s)	41, 114, 735, 1203, 1356
TauI	GCSGC	1 cut(s)	788
TfiI	GAWTC	4 cut(s)	28, 209, 431, 1160
Tru1I	TTAA	5 cut(s)	567, 821, 1023, 1247, 1284
Tru9I	TTAA	5 cut(s)	567, 821, 1023, 1247, 1284
TscAI	CASTG	5 cut(s)	315, 640, 772, 955, 987
TseFI	GTSAC	4 cut(s)	720, 767, 842, 1659
TseI	GCWGC	9 cut(s)	534, 656, 986, 1517, 1619, 1622, 1625, 1628, 1667
Tsp45I	GTSAC	4 cut(s)	720, 767, 842, 1659
TspDTI	ATGAA	7 cut(s)	209, 285, 326, 527, 878, 911, 1146
TspGWI	ACGGA	2 cut(s)	136, 492
TspRI	CASTG	5 cut(s)	315, 640, 772, 955, 987
VpaK11BI	GGWCC	2 cut(s)	479, 1451
XagI	CCTNNNNNAGG	1 cut(s)	1187
XapI	RAATTY	4 cut(s)	141, 216, 438, 1133
XceI	RCATGY	1 cut(s)	448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1046
XmaJI	CCTAGG	1 cut(s)	361
XmiI	GTMKAC	1 cut(s)	609
XspI	CTAG	2 cut(s)	362, 1410
ZrmI	AGTACT	1 cut(s)	43

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.