RchiOBHm_Chr3g0472681

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006498
Physical Location & Seq
Reverse (-)
18605555 .. 18606733
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43837

Sequence Viewer

Length: 627 bp
ATGTGGATCTTACGATATCTAGTGCTAGTATTGGTATCCAGTACTTGTGTTGTAGCTACATTGGCTCAAGGAACAAACGAGGATGGTTCTGGCGTTAGGAAGCCTTACATTGTGTACATGGGAGAACTGCCGGAACCCGGATCCTCCACCATGGTCATGACCACAGCCGGCCATGATAACTTGCTCAAGACAGCAATTGGAGATGCCGACTTAGCCAGAGTATCCAAAATTTATACTTATGGAAGGAGCTTCAATGGCTTCGTAGCCAGATTGTTGCCGCATGAAGCAAAGAAACTATCAGATGATGATCGGGTTGTTTCAGTGTTTCCAAACACCAAGCTCAAATTACATACAACAAGGTCATGGGATTTCCTAGGATTGCCAGGAAATACGAAGAAACGAAACTTGCCAATAGAGAGCAAGATCGTAGTGGGTGTGATAGATACAGGAATTTGGCCGGCTTGTCCCAGTATGAACGATTCAGGCTTCGGACCACCCCCAAGAAAATGGAAAGGAAAATGTATGAAGGGCGGTAACTTCACCGGCTGTAACAAGTATATTTCTCTCTCTCACACCAGATCATTTCATTGTAAGTTTCGATTTTGTACCTTATTAGACAATCAATGA

Protein Analysis

208

Amino Acids

23.0

Weight (kDa)

9.51

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 117 1.4e-15 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 278, 531
AclWI GGATC 3 cut(s) 14, 135, 148
AcoI YGGCCR 2 cut(s) 169, 455
AcsI RAATTY 2 cut(s) 228, 450
AfaI GTAC 3 cut(s) 43, 116, 607
AfiI CCNNNNNNNGG 1 cut(s) 137
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 382
AluBI AGCT 3 cut(s) 56, 249, 340
AluI AGCT 3 cut(s) 56, 249, 340
AlwI GGATC 3 cut(s) 14, 135, 148
AoxI GGCC 2 cut(s) 169, 455
ApoI RAATTY 2 cut(s) 228, 450
AspA2I CCTAGG 1 cut(s) 373
AspS9I GGNCC 1 cut(s) 491
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 532
AvaII GGWCC 1 cut(s) 491
AvrII CCTAGG 1 cut(s) 373
BamHI GGATCC 1 cut(s) 140
BccI CCATC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 384
BciVI GTATCC 2 cut(s) 46, 232
BcnI CCSGG 1 cut(s) 138
BfaI CTAG 3 cut(s) 20, 26, 374
BfuI GTATCC 2 cut(s) 46, 232
BisI GCNGC 1 cut(s) 278
BlnI CCTAGG 1 cut(s) 373
BlsI GCNGC 1 cut(s) 279
BmcAI AGTACT 1 cut(s) 43
Bme1390I CCNGG 2 cut(s) 138, 384
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 2 cut(s) 135, 142
BmrFI CCNGG 2 cut(s) 138, 384
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 1 cut(s) 193
BmuI ACTGGG 1 cut(s) 462
BpuEI CTTGAG 2 cut(s) 51, 170
BpuMI CCSGG 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 150, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse118I RCCGGY 3 cut(s) 167, 457, 542
Bse1I ACTGG 2 cut(s) 39, 468
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 384
BseDI CCNNGG 2 cut(s) 150, 373
BseGI GGATG 1 cut(s) 88
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 137
BseNI ACTGG 2 cut(s) 39, 468
BshFI GGCC 2 cut(s) 171, 457
BsiSI CCGG 5 cut(s) 131, 138, 168, 458, 543
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 2 cut(s) 171, 457
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 5 cut(s) 6, 140, 307, 423, 578
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 2 cut(s) 278, 531
BspANI GGCC 2 cut(s) 171, 457
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 135, 142
BspPI GGATC 3 cut(s) 14, 135, 148
BsrFI RCCGGY 3 cut(s) 167, 457, 542
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 2 cut(s) 39, 468
BssAI RCCGGY 3 cut(s) 167, 457, 542
BssECI CCNNGG 2 cut(s) 150, 373
BssMI GATC 5 cut(s) 6, 140, 307, 423, 578
BssT1I CCWWGG 2 cut(s) 150, 373
Bst2UI CCWGG 1 cut(s) 384
BstAUI TGTACA 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 169, 459
BstDEI CTNAG 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 5 cut(s) 9, 143, 310, 426, 581
BstMBI GATC 5 cut(s) 6, 140, 307, 423, 578
BstMWI GCNNNNNNNGC 3 cut(s) 62, 212, 255
BstNI CCWGG 1 cut(s) 384
BstSCI CCNGG 2 cut(s) 136, 382
BstX2I RGATCY 2 cut(s) 6, 140
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 2 cut(s) 6, 140
BsuI GTATCC 2 cut(s) 46, 232
BsuRI GGCC 2 cut(s) 171, 457
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 2 cut(s) 169, 459
CciI TCATGA 1 cut(s) 156
Cfr10I RCCGGY 3 cut(s) 167, 457, 542
Cfr13I GGNCC 1 cut(s) 491
Csp6I GTAC 3 cut(s) 42, 115, 606
CviAII CATG 6 cut(s) 118, 151, 157, 173, 281, 363
CviQI GTAC 3 cut(s) 42, 115, 606
DdeI CTNAG 1 cut(s) 211
DpnI GATC 5 cut(s) 8, 142, 309, 425, 580
DpnII GATC 5 cut(s) 6, 140, 307, 423, 578
EaeI YGGCCR 2 cut(s) 169, 455
Eco130I CCWWGG 2 cut(s) 150, 373
Eco32I GATATC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 382
EcoRV GATATC 1 cut(s) 17
EcoT14I CCWWGG 2 cut(s) 150, 373
ErhI CCWWGG 2 cut(s) 150, 373
FaeI CATG 6 cut(s) 121, 154, 160, 176, 284, 366
FaqI GGGAC 1 cut(s) 450
FatI CATG 6 cut(s) 117, 150, 156, 172, 280, 362
Fnu4HI GCNGC 1 cut(s) 278
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 1 cut(s) 278
FspBI CTAG 3 cut(s) 20, 26, 374
GluI GCNGC 1 cut(s) 278
HaeIII GGCC 2 cut(s) 171, 457
HapII CCGG 5 cut(s) 131, 138, 168, 458, 543
Hin1II CATG 6 cut(s) 121, 154, 160, 176, 284, 366
HinfI GANTC 1 cut(s) 479
HpaII CCGG 5 cut(s) 131, 138, 168, 458, 543
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 2 cut(s) 301, 491
Hpy188III TCNNGA 2 cut(s) 157, 187
Hpy8I GTNNAC 1 cut(s) 115
HpyAV CCTTC 2 cut(s) 237, 520
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 212, 255
HpyF3I CTNAG 1 cut(s) 211
Hsp92II CATG 6 cut(s) 121, 154, 160, 176, 284, 366
KroI GCCGGC 2 cut(s) 167, 457
KroNI GCCGGC 2 cut(s) 169, 459
Kzo9I GATC 5 cut(s) 6, 140, 307, 423, 578
LmnI GCTCC 1 cut(s) 246
LweI GCATC 1 cut(s) 193
MaeI CTAG 3 cut(s) 20, 26, 374
MaeIII GTNAC 2 cut(s) 533, 548
MalI GATC 5 cut(s) 8, 142, 309, 425, 580
MboI GATC 5 cut(s) 6, 140, 307, 423, 578
MboII GAAGA 1 cut(s) 406
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 2 cut(s) 6, 140
MluCI AATT 4 cut(s) 195, 228, 344, 450
MnlI CCTC 2 cut(s) 73, 154
MroNI GCCGGC 2 cut(s) 167, 457
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 5 cut(s) 131, 138, 168, 458, 543
MspR9I CCNGG 2 cut(s) 138, 384
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 3 cut(s) 62, 212, 255
NaeI GCCGGC 2 cut(s) 169, 459
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 150
NdeII GATC 5 cut(s) 6, 140, 307, 423, 578
NgoMIV GCCGGC 2 cut(s) 167, 457
NlaIII CATG 6 cut(s) 121, 154, 160, 176, 284, 366
NlaIV GGNNCC 2 cut(s) 135, 142
PagI TCATGA 1 cut(s) 156
PdiI GCCGGC 2 cut(s) 169, 459
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 382
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 2 cut(s) 135, 142
PspPI GGNCC 1 cut(s) 491
PsuI RGATCY 2 cut(s) 6, 140
RsaI GTAC 3 cut(s) 43, 116, 607
RsaNI GTAC 3 cut(s) 42, 115, 606
RseI CAYNNNNRTG 1 cut(s) 155
SatI GCNGC 1 cut(s) 278
Sau3AI GATC 5 cut(s) 6, 140, 307, 423, 578
Sau96I GGNCC 1 cut(s) 491
ScaI AGTACT 1 cut(s) 43
ScrFI CCNGG 2 cut(s) 138, 384
SetI ASST 5 cut(s) 58, 251, 342, 362, 611
SfaNI GCATC 1 cut(s) 193
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 66, 185
SmoI CTYRAG 2 cut(s) 66, 185
Sse9I AATT 4 cut(s) 195, 228, 344, 450
SsiI CCGC 2 cut(s) 278, 531
SspMI CTAG 3 cut(s) 20, 26, 374
StyD4I CCNGG 2 cut(s) 136, 382
StyI CCWWGG 2 cut(s) 150, 373
TaqI TCGA 1 cut(s) 598
TasI AATT 4 cut(s) 195, 228, 344, 450
TatI WGTACW 2 cut(s) 41, 114
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 479
TscAI CASTG 1 cut(s) 327
TspDTI ATGAA 4 cut(s) 297, 488, 539, 575
TspRI CASTG 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 2 cut(s) 228, 450
XmaJI CCTAGG 1 cut(s) 373
XspI CTAG 3 cut(s) 20, 26, 374
ZrmI AGTACT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.