Rh3CG202800

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0006498
Physical Location & Seq
Reverse (-)
18349546 .. 18352229
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG202800.1

Sequence Viewer

Length: 1047 bp
ATGTGGATCTTACGATATCTAGTGCTAGTATTGGTATCCAGTACTTGTGTTGTAGCTACATTGGCTCAAGGAACAAACGAGGATGGTTCTGGCGTTAGGAAGCCTTACATTGTGTACATGGGAGAACTGCCGGAACCCGGATCCTCCACCATGGTCATGACCACAGCCGGCCATGATAACTTGCTCAAGACAGCAATTGGAGATGCCGACTTAGCCAGAGTATCCAAAATTTATACTTATGGAAGGAGCTTCAATGGCTTCGTAGCCAGATTGTTGCCGCATGAAGCAAAGAAACTATCAGATGATGATCGGGTTGTTTCAGTGTTTCCAAACACCAAGCTCAAATTACATACAACAAGGTCATGGGATTTCCTAGGATTGCCAGGAAATACGAAGAAACGAAACTTGCCAATAGAGAGCAAGATCGTAGTGGGTGTGATAGATACAGGAATTTGGCCGGCTTGTCCCAGTATGAACGATTCAGGCTTCGGACCACCCCCAAGAAAATGGAAAGGAAAATGTATGAAGGGCGGTAACTTCACCGGCTGTAACAACAAGGTAATTGGTGCCAAATACTTCATGGTGGACGACATGCCCGGCCCTGGTTATGAGGACAACGCTAGTCCGAATGTTGCACCATGGATAATGACTGTGGCTGCTACTGGCCTTGATAGGCGGTTAAAGACTCTAGTTAAGTTGGGCAATGGCAAGAAAATTTCTGGAAATTCAATCAATACCTTCACCCCCAAGAAACAGATGTATCCCCTTACAAGTGGAGCCCGTGCTTCCAACGTTACTTCAGACCTCTACGGAAATGCAAGTGCTTGTGACACTGGGACTTTAAACCCTCAGAAGGTGAAGGGAAGGATTGTGTTGTGCAAAGGGCAAAGTAATCAGGATCCAACTATTAAAGACTTAAATGGGGCAGGAACTATTATGACAGAAGATGAAATGCTTGATTACGGTTACACCCCTATCATCCCAGCTACCTATGTGCTTGTTAATGATGGACTTAAGATTGAGCGGTATATAAACACTACCAAGTAA

Protein Analysis

348

Amino Acids

37.78

Weight (kDa)

9.31

Isoelectric Point (pI)

24.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 117 3.9e-15 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 1024
AciI CCGC 4 cut(s) 278, 531, 676, 1024
AclI AACGTT 1 cut(s) 792
AclWI GGATC 5 cut(s) 14, 135, 148, 893, 906
AcoI YGGCCR 2 cut(s) 169, 455
AcsI RAATTY 4 cut(s) 228, 450, 714, 724
AcuI CTGAAG 1 cut(s) 783
AfaI GTAC 2 cut(s) 43, 116
AfiI CCNNNNNNNGG 3 cut(s) 137, 602, 853
AflII CTTAAG 1 cut(s) 1013
AgsI TTSAA 2 cut(s) 253, 729
AjnI CCWGG 2 cut(s) 382, 601
AluBI AGCT 4 cut(s) 56, 249, 340, 986
AluI AGCT 4 cut(s) 56, 249, 340, 986
AlwI GGATC 5 cut(s) 14, 135, 148, 893, 906
AoxI GGCC 4 cut(s) 169, 455, 598, 664
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 4 cut(s) 228, 450, 714, 724
AspA2I CCTAGG 1 cut(s) 373
AspS9I GGNCC 2 cut(s) 491, 599
AsuC2I CCSGG 2 cut(s) 138, 597
AsuHPI GGTGA 3 cut(s) 532, 733, 868
AvaII GGWCC 1 cut(s) 491
AvrII CCTAGG 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 743, 776
BamHI GGATCC 2 cut(s) 140, 898
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 1 cut(s) 781
BbvI GCAGC 1 cut(s) 643
BccI CCATC 2 cut(s) 77, 1001
BciT130I CCWGG 2 cut(s) 384, 603
BciVI GTATCC 3 cut(s) 46, 232, 771
BcnI CCSGG 2 cut(s) 138, 597
BfaI CTAG 5 cut(s) 20, 26, 374, 621, 689
BfrI CTTAAG 1 cut(s) 1013
BfuI GTATCC 3 cut(s) 46, 232, 771
BisI GCNGC 2 cut(s) 278, 657
BlnI CCTAGG 1 cut(s) 373
BlsI GCNGC 2 cut(s) 279, 658
BmcAI AGTACT 1 cut(s) 43
Bme1390I CCNGG 4 cut(s) 138, 384, 597, 603
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 2 cut(s) 491, 599
BmiI GGNNCC 5 cut(s) 135, 142, 568, 778, 900
BmrFI CCNGG 4 cut(s) 138, 384, 597, 603
BmrI ACTGGG 2 cut(s) 462, 843
BmsI GCATC 1 cut(s) 193
BmuI ACTGGG 2 cut(s) 462, 843
BpuEI CTTGAG 2 cut(s) 51, 170
BpuMI CCSGG 2 cut(s) 138, 597
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 150, 373, 601, 638
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 602, 853
Bse118I RCCGGY 3 cut(s) 167, 457, 542
Bse1I ACTGG 4 cut(s) 39, 468, 667, 838
Bse3DI GCAATG 1 cut(s) 709
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 384, 603
BseDI CCNNGG 4 cut(s) 150, 373, 601, 638
BseGI GGATG 2 cut(s) 88, 978
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 3 cut(s) 137, 602, 853
BseMI GCAATG 1 cut(s) 709
BseMII CTCAG 1 cut(s) 863
BseNI ACTGG 4 cut(s) 39, 468, 667, 838
BseXI GCAGC 1 cut(s) 643
BseYI CCCAGC 1 cut(s) 982
BshFI GGCC 4 cut(s) 171, 457, 600, 666
BshNI GGYRCC 1 cut(s) 566
BsiSI CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
BslFI GGGAC 2 cut(s) 450, 850
BslI CCNNNNNNNGG 3 cut(s) 137, 602, 853
BsmFI GGGAC 2 cut(s) 450, 850
BsnI GGCC 4 cut(s) 171, 457, 600, 666
Bsp1286I GDGCHC 1 cut(s) 781
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 5 cut(s) 6, 140, 307, 423, 898
Bsp19I CCATGG 2 cut(s) 150, 638
BspACI CCGC 4 cut(s) 278, 531, 676, 1024
BspANI GGCC 4 cut(s) 171, 457, 600, 666
BspCNI CTCAG 1 cut(s) 862
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 5 cut(s) 135, 142, 568, 778, 900
BspPI GGATC 5 cut(s) 14, 135, 148, 893, 906
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 1013
BsrBI CCGCTC 1 cut(s) 1024
BsrDI GCAATG 1 cut(s) 709
BsrFI RCCGGY 3 cut(s) 167, 457, 542
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 4 cut(s) 39, 468, 667, 838
BssAI RCCGGY 3 cut(s) 167, 457, 542
BssECI CCNNGG 4 cut(s) 150, 373, 601, 638
BssMI GATC 5 cut(s) 6, 140, 307, 423, 898
BssT1I CCWWGG 3 cut(s) 150, 373, 638
Bst2UI CCWGG 2 cut(s) 384, 603
Bst4CI ACNGT 2 cut(s) 652, 965
BstAFI CTTAAG 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 169, 459
BstDEI CTNAG 2 cut(s) 211, 849
BstDSI CCRYGG 2 cut(s) 150, 638
BstF5I GGATG 2 cut(s) 88, 978
BstKTI GATC 5 cut(s) 9, 143, 310, 426, 901
BstMBI GATC 5 cut(s) 6, 140, 307, 423, 898
BstMWI GCNNNNNNNGC 3 cut(s) 62, 212, 255
BstNI CCWGG 2 cut(s) 384, 603
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 4 cut(s) 136, 382, 595, 601
BstV1I GCAGC 1 cut(s) 643
BstX2I RGATCY 3 cut(s) 6, 140, 898
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 3 cut(s) 6, 140, 898
BsuI GTATCC 3 cut(s) 46, 232, 771
BsuRI GGCC 4 cut(s) 171, 457, 600, 666
BtgI CCRYGG 2 cut(s) 150, 638
BtsCI GGATG 2 cut(s) 88, 978
BtsIMutI CAGTG 2 cut(s) 327, 831
Cac8I GCNNGC 2 cut(s) 169, 459
CciI TCATGA 1 cut(s) 156
Cfr10I RCCGGY 3 cut(s) 167, 457, 542
Cfr13I GGNCC 2 cut(s) 491, 599
Csp6I GTAC 2 cut(s) 42, 115
CviAII CATG 9 cut(s) 118, 151, 157, 173, 281, 363, 580, 592, 639
CviQI GTAC 2 cut(s) 42, 115
DdeI CTNAG 2 cut(s) 211, 849
DpnI GATC 5 cut(s) 8, 142, 309, 425, 900
DpnII GATC 5 cut(s) 6, 140, 307, 423, 898
DraI TTTAAA 1 cut(s) 843
EaeI YGGCCR 2 cut(s) 169, 455
Eco130I CCWWGG 3 cut(s) 150, 373, 638
Eco24I GRGCYC 1 cut(s) 781
Eco32I GATATC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 783
EcoRII CCWGG 2 cut(s) 382, 601
EcoRV GATATC 1 cut(s) 17
EcoT14I CCWWGG 3 cut(s) 150, 373, 638
EcoT38I GRGCYC 1 cut(s) 781
ErhI CCWWGG 3 cut(s) 150, 373, 638
FaeI CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 642
FaqI GGGAC 2 cut(s) 450, 850
FatI CATG 9 cut(s) 117, 150, 156, 172, 280, 362, 579, 591, 638
Fnu4HI GCNGC 2 cut(s) 278, 657
FokI GGATG 2 cut(s) 95, 965
FriOI GRGCYC 1 cut(s) 781
Fsp4HI GCNGC 2 cut(s) 278, 657
FspBI CTAG 5 cut(s) 20, 26, 374, 621, 689
GluI GCNGC 2 cut(s) 278, 657
GsaI CCCAGC 1 cut(s) 986
HaeIII GGCC 4 cut(s) 171, 457, 600, 666
HapII CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
Hin1II CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 642
HinfI GANTC 2 cut(s) 479, 685
HpaII CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
HphI GGTGA 3 cut(s) 532, 733, 868
Hpy166II GTNNAC 2 cut(s) 115, 586
Hpy188I TCNGA 5 cut(s) 301, 491, 627, 802, 852
Hpy188III TCNNGA 4 cut(s) 157, 187, 720, 896
Hpy8I GTNNAC 2 cut(s) 115, 586
HpyAV CCTTC 6 cut(s) 237, 520, 748, 847, 853, 858
HpyCH4III ACNGT 2 cut(s) 652, 965
HpyCH4IV ACGT 1 cut(s) 792
HpyCH4V TGCA 3 cut(s) 635, 818, 879
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 212, 255
HpyF3I CTNAG 2 cut(s) 211, 849
HpySE526I ACGT 1 cut(s) 792
Hsp92II CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 642
KroI GCCGGC 2 cut(s) 167, 457
KroNI GCCGGC 2 cut(s) 169, 459
Kzo9I GATC 5 cut(s) 6, 140, 307, 423, 898
LmnI GCTCC 2 cut(s) 246, 776
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 1 cut(s) 193
MaeI CTAG 5 cut(s) 20, 26, 374, 621, 689
MaeII ACGT 1 cut(s) 792
MaeIII GTNAC 5 cut(s) 533, 548, 793, 827, 965
MalI GATC 5 cut(s) 8, 142, 309, 425, 900
MbiI CCGCTC 1 cut(s) 1024
MboI GATC 5 cut(s) 6, 140, 307, 423, 898
MboII GAAGA 2 cut(s) 406, 956
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 3 cut(s) 6, 140, 898
MhlI GDGCHC 1 cut(s) 781
MluCI AATT 7 cut(s) 195, 228, 344, 450, 561, 714, 724
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 2 cut(s) 813, 926
MnlI CCTC 5 cut(s) 73, 154, 604, 815, 858
MroNI GCCGGC 2 cut(s) 167, 457
MseI TTAA 7 cut(s) 680, 693, 842, 909, 917, 1002, 1014
MslI CAYNNNNRTG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 1013
MspI CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
MspR9I CCNGG 4 cut(s) 138, 384, 597, 603
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 2 cut(s) 384, 603
MwoI GCNNNNNNNGC 3 cut(s) 62, 212, 255
NaeI GCCGGC 2 cut(s) 169, 459
NciI CCSGG 2 cut(s) 138, 597
NcoI CCATGG 2 cut(s) 150, 638
NdeII GATC 5 cut(s) 6, 140, 307, 423, 898
NgoMIV GCCGGC 2 cut(s) 167, 457
NlaIII CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 642
NlaIV GGNNCC 5 cut(s) 135, 142, 568, 778, 900
NmuCI GTSAC 1 cut(s) 827
NspI RCATGY 1 cut(s) 595
PagI TCATGA 1 cut(s) 156
PdiI GCCGGC 2 cut(s) 169, 459
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 279, 658
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
Psp1406I AACGTT 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 382, 601
PspFI CCCAGC 1 cut(s) 982
PspGI CCWGG 2 cut(s) 382, 601
PspN4I GGNNCC 5 cut(s) 135, 142, 568, 778, 900
PspPI GGNCC 2 cut(s) 491, 599
PsuI RGATCY 3 cut(s) 6, 140, 898
RsaI GTAC 2 cut(s) 43, 116
RsaNI GTAC 2 cut(s) 42, 115
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 7 cut(s) 680, 693, 842, 909, 917, 1002, 1014
SatI GCNGC 2 cut(s) 278, 657
Sau3AI GATC 5 cut(s) 6, 140, 307, 423, 898
Sau96I GGNCC 2 cut(s) 491, 599
ScaI AGTACT 1 cut(s) 43
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 4 cut(s) 138, 384, 597, 603
SduI GDGCHC 1 cut(s) 781
SfaNI GCATC 1 cut(s) 193
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 3 cut(s) 66, 185, 1013
SmoI CTYRAG 3 cut(s) 66, 185, 1013
Sse9I AATT 7 cut(s) 195, 228, 344, 450, 561, 714, 724
SsiI CCGC 4 cut(s) 278, 531, 676, 1024
SspMI CTAG 5 cut(s) 20, 26, 374, 621, 689
StyD4I CCNGG 4 cut(s) 136, 382, 595, 601
StyI CCWWGG 3 cut(s) 150, 373, 638
TaaI ACNGT 2 cut(s) 652, 965
TaiI ACGT 1 cut(s) 795
TasI AATT 7 cut(s) 195, 228, 344, 450, 561, 714, 724
TatI WGTACW 2 cut(s) 41, 114
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 7 cut(s) 680, 693, 842, 909, 917, 1002, 1014
Tru9I TTAA 7 cut(s) 680, 693, 842, 909, 917, 1002, 1014
TscAI CASTG 2 cut(s) 327, 838
TseFI GTSAC 1 cut(s) 827
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 1 cut(s) 827
TspDTI ATGAA 5 cut(s) 297, 488, 539, 568, 963
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 2 cut(s) 327, 838
Vha464I CTTAAG 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 4 cut(s) 228, 450, 714, 724
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmaJI CCTAGG 1 cut(s) 373
XspI CTAG 5 cut(s) 20, 26, 374, 621, 689
ZrmI AGTACT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.