MD14G1019300.v1.1

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006498
Physical Location & Seq
Reverse (-)
1812376 .. 1812912
537 bp
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UTR
Exon/CDS
Intron
MD14G1019300.v1.1.491

Sequence Viewer

Length: 537 bp
ATGAAAAATGAAACAGCGGATACCCAGCTAGCCACGGGAGCAGGACAAGTAAACCCAAAAAAGGCAGTGCATCCTGGTCTTGTCTACGACATAACCCCAGATGACTACATTAGTTTCTTGTGCAAAGAAGGTTTCAACGACACAGACATAGGCACACTTATTGGGGATAAAAAGAAGTACAAATGCTCAGACTTTAAACATCCACGAGGCACAGACGGCCTCAACTATCCTTCTATGCATCTTCAGCTTTTCCCGAACGACAATTATGGCGTTTCAGCCGTCTTCTATAGAAGAGTGACGCATGTAGGGTTCGGTAATTCCGTGTACAAGGCAAATGTGACGAATTTGAATGGTTTGAGAGTGAAAGTCATACCGAGTACCTTGACCTTCACTAAGTTGCATCAGAAAAGGTCCTTTAAGGTTGTGGTGACTGGTCGTCCAGCGATGAACGAGACAGTTATGCACGCTTTACTCCAATGGGATGACTCTAAACACAACGTTAAGAGCCATATCGTAGTCTATAAGCATCTGAATTAG

Protein Analysis

179

Amino Acids

19.99

Weight (kDa)

9.45

Isoelectric Point (pI)

24.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 74 - 173 1.4e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 84
AciI CCGC 1 cut(s) 17
AclI AACGTT 1 cut(s) 498
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 227
AfaI GTAC 3 cut(s) 179, 326, 379
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 2 cut(s) 136, 349
AhdI GACNNNNNGTC 1 cut(s) 435
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 2 cut(s) 28, 247
AluI AGCT 2 cut(s) 28, 247
Alw26I GTCTC 1 cut(s) 446
AoxI GGCC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 439
AsuNHI GCTAGC 1 cut(s) 28
AvaII GGWCC 1 cut(s) 411
BauI CACGAG 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 274
BceAI ACGGC 2 cut(s) 232, 263
BciT130I CCWGG 1 cut(s) 75
BciVI GTATCC 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 286
BfuI GTATCC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 411
BmeRI GACNNNNNGTC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 3 cut(s) 79, 247, 409
BmtI GCTAGC 1 cut(s) 32
BpiI GAAGAC 1 cut(s) 274
BsaJI CCNNGG 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 70, 199, 487
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 436
BseYI CCCAGC 1 cut(s) 24
BshFI GGCC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 446
BsnI GGCC 1 cut(s) 219
Bsp1407I TGTACA 1 cut(s) 324
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 200
BspOI GCTAGC 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 324
BsrI ACTGG 1 cut(s) 436
BssECI CCNNGG 1 cut(s) 33
BssSI CACGAG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 1 cut(s) 286
BstAUI TGTACA 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 30, 465
BstDEI CTNAG 2 cut(s) 187, 393
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 70, 199, 487
BstMAI GTCTC 1 cut(s) 446
BstMWI GCNNNNNNNGC 3 cut(s) 38, 216, 244
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 274
BsuI GTATCC 1 cut(s) 13
BsuRI GGCC 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 33
BtgZI GCGATG 1 cut(s) 458
BtsCI GGATG 3 cut(s) 70, 199, 487
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 30, 465
Cfr13I GGNCC 1 cut(s) 411
CseI GACGC 1 cut(s) 307
Csp6I GTAC 3 cut(s) 178, 325, 378
CviAII CATG 1 cut(s) 302
CviJI RGCY 6 cut(s) 28, 32, 219, 247, 278, 507
CviKI_1 RGCY 6 cut(s) 28, 32, 219, 247, 278, 507
CviQI GTAC 3 cut(s) 178, 325, 378
DdeI CTNAG 2 cut(s) 187, 393
DraI TTTAAA 1 cut(s) 196
DriI GACNNNNNGTC 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 240
FaeI CATG 1 cut(s) 305
FatI CATG 1 cut(s) 301
FblI GTMKAC 1 cut(s) 84
FokI GGATG 3 cut(s) 57, 186, 494
FspBI CTAG 1 cut(s) 29
GsaI CCCAGC 1 cut(s) 28
HaeIII GGCC 1 cut(s) 219
HgaI GACGC 1 cut(s) 307
Hin1II CATG 1 cut(s) 305
HinfI GANTC 1 cut(s) 485
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 3 cut(s) 52, 85, 325
Hpy188I TCNGA 3 cut(s) 190, 405, 531
Hpy188III TCNNGA 1 cut(s) 253
Hpy8I GTNNAC 3 cut(s) 52, 85, 325
HpyAV CCTTC 3 cut(s) 122, 240, 397
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 5 cut(s) 70, 123, 238, 400, 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 216, 244
HpyF3I CTNAG 2 cut(s) 187, 393
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 1 cut(s) 305
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 7 cut(s) 27, 38, 60, 87, 111, 417, 453
LweI GCATC 3 cut(s) 79, 247, 409
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 3 cut(s) 295, 337, 427
MboII GAAGA 3 cut(s) 233, 274, 303
MluCI AATT 4 cut(s) 262, 316, 343, 532
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 2 cut(s) 200, 230
Mph1103I ATGCAT 1 cut(s) 240
MseI TTAA 3 cut(s) 195, 417, 501
MspA1I CMGCKG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 3 cut(s) 38, 216, 244
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 1 cut(s) 305
NmuCI GTSAC 3 cut(s) 295, 337, 427
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 411
Psp1406I AACGTT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 1 cut(s) 73
PspPI GGNCC 1 cut(s) 411
PspPPI RGGWCCY 1 cut(s) 411
RsaI GTAC 3 cut(s) 179, 326, 379
RsaNI GTAC 3 cut(s) 178, 325, 378
SaqAI TTAA 3 cut(s) 195, 417, 501
Sau96I GGNCC 1 cut(s) 411
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 1 cut(s) 75
SetI ASST 8 cut(s) 30, 133, 249, 383, 389, 413, 423, 501
SfaNI GCATC 3 cut(s) 79, 247, 409
SfcI CTRYAG 1 cut(s) 286
SinI GGWCC 1 cut(s) 411
Sse9I AATT 4 cut(s) 262, 316, 343, 532
SsiI CCGC 1 cut(s) 17
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 1 cut(s) 501
TasI AATT 4 cut(s) 262, 316, 343, 532
TatI WGTACW 2 cut(s) 177, 324
Tru1I TTAA 3 cut(s) 195, 417, 501
Tru9I TTAA 3 cut(s) 195, 417, 501
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 3 cut(s) 295, 337, 427
Tsp45I GTSAC 3 cut(s) 295, 337, 427
TspDTI ATGAA 3 cut(s) 17, 24, 461
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 1 cut(s) 343
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.