Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0006498

Physical Location & Seq

Genomic Coordinates Forward (+)

31242975 .. 31245235

Gene Structure 2261 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00408730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGCCCGGCCCCAGTTACGAGGACAACGCTAGTCCAGTTGACACTGATGGCCATGGCACTCACACAGCCTCCATTGTTGGTGGTGTACCCGTCCGAGGAGCCAGCCTATATGGCATTGCAAAAGGGACTGCTCGTGGGGGAGTGCCATCGTCACGTATAGCCACGTACAAAGCATGCTGGACTGGTGGCTGCGGTGACATGAACATCTTGGCCGCGTTTGATGAAGCCATTGCTGATGGTGTGGACTTGATATCAATCTCCGTTGGCGGTCCTTCACGGAATTTCCTCCAGGATTCCATTGCTCTCGGATCATTTCATGCCATGAAGAGAGGGATTTTGGTTTCAGCCTCAGCTGGGAATGATGGACCTTATGCATCCTCTGTTCAGAATGTTGCACCATGGATAATGACTGTGGCTGCTACTGGCCTTGATAGGCGGTTAAAGACTCTAGTTAAGTTGGGTAATGGCAAGAAAATTTCTGGAAATTCAATCAATACCTTCACCCCCAAGAAACAGATGTATCCCCTCACAAGTGGAGCCCGTGCTTCCAACGTTACTTCAGACCTCTACGGAAATGCGAGTGCTTGTGACAGTGGGACTTTAAACCCTCAGAAGGTGAAGGGAAGGATTGTGTTGTGCAAAGGGCAAAGTAATCAGGATCCAACTATTAAAGACTTAAATGGGGCAGGAACTATAATGACAGAAGATGAAATGCTTGATTACGGTTACACCCCTATCATCCCAGCTACCTATGTGCTTGTTAATGATGGACTTAAGATCGAGCGGAACCCTACGGCTGTAATACAGCGGACAAGAACTGTTCATATGGATGCCCCCTTCATTGCTTCTTTCTCTTCCAGAGGACCTCAACTCATAACCCCCAACATCCTCAAGCCTGATCTTGCTGCACCAGGGATAAACATACTAGCTGCATATTCGAAGCTGCCATCTTTAACAGGTAGCCCAGAAGACATGCGCCACTATTCGTTCAACATACTCTCAGGTACATCCATGTCTTGCCCTCATGCAACTGCCTCAGCAGCCTATGTCAAGTCCTTCCACCCAGACTGGTCCCCGGCTGCCATCAAGTCGGCTCTCATGACAACCGCCACACCATTGAAAATCAAACCCCTGGAAGGCGAGTTAGCCTTCGGAGCAGGACAAATACAGCCAATAAAGGCAGTGCATCCTGGCCTAGTCTACGACATTTCCATGAACGACTACATCAGCTTCTTGTGCAAGGAAGGCTACAACAGCACCAGCATTGGAAAGCTTATTGGTGGTAAAAAGAAGTACAACTGCTCAGACTTTAAGCCCGCACCCGGCACCGATGGTCTCAACTATCCTTCTATGTCCTACCTGGTGCGAAATCCGAATTCTAGCATTTCAGCTGTTTTCCTTAGATCGGTGAAGCATGTAGGGTACGGTAAGTCGGTGTACAAGGCAAAGGTGACTTCGCCAAAGGACCTTTCCATTAGAGTCACCCCGAGTAACTTGACATTCACTAAGAAGCATCAGAAGCGATCCTTCACGGTTGTGGTAAAGGGTGGCACGATGAAAGGAAAGACAGAGAGTCTGTCTGCTCTACTAGAGTGGGATGATGGTAGGCATAGGGTTAGGAGCCCCATCGTAGTCTACAGGCATACCACATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

551

Amino Acids

58.6

Weight (kDa)

9.52

Isoelectric Point (pI)

35.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	6 - 385	4e-41	Subtilase family
fn3_6	PF17766	446 - 545	4e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006498)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03620 AT5G03620
malus_domestica	MD12G1022400.v1.1 MD12G1022500.v1.1 MD14G1019300.v1.1 MD14G1019400.v1.1
prunus_persica	Prupe.7G114300_v2.0.a1 Prupe.7G114300_v2.0.a1
pyrus_communis	pycom14g01900
rosa_chinensis	RchiOBHm_Chr3g0472671 RchiOBHm_Chr3g0472681
rosa_laevigata	RLG00000016130 RLG00000024064
rosa_multiflora	Rmu_sc0001616.1_g000054 Rmu_sc0003510.1_g000013
rosa_roxburghii	Rroxscaffold_5G00353850 Rroxscaffold_6G00408720 Rroxscaffold_6G00408730
rosa_rugosa	Rorug03G0127100 Rorug04G0092600
rosa_samantha	Rh3AG179800 Rh3BG205700 Rh3CG202800 Rh3DG170000 Rh3DG170100
rosa_wichuraiana	Rw3G016290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1325
AccBSI	CCGCTC	1 cut(s)	784
AccI	GTMKAC	2 cut(s)	1200, 1635
AccII	CGCG	1 cut(s)	215
AciI	CCGC	8 cut(s)	192, 213, 267, 436, 784, 808, 1107, 1317
AclI	AACGTT	1 cut(s)	552
AclWI	GGATC	4 cut(s)	316, 653, 666, 1518
AcoI	YGGCCR	2 cut(s)	49, 210
AcsI	RAATTY	4 cut(s)	280, 474, 484, 1375
AcuI	CTGAAG	1 cut(s)	543
AfaI	GTAC	6 cut(s)	87, 167, 1006, 1295, 1424, 1439
AfiI	CCNNNNNNNGG	6 cut(s)	95, 354, 613, 1136, 1322, 1405
AflII	CTTAAG	1 cut(s)	773
AgsI	TTSAA	3 cut(s)	489, 991, 1120
AhdI	GACNNNNNGTC	1 cut(s)	1572
AjnI	CCWGG	5 cut(s)	288, 910, 1131, 1189, 1359
AjuI	GAANNNNNNNTTGG	2 cut(s)	1453, 1485
AleI	CACNNNNGTG	1 cut(s)	1535
AluBI	AGCT	7 cut(s)	353, 746, 929, 943, 1230, 1273, 1391
AluI	AGCT	7 cut(s)	353, 746, 929, 943, 1230, 1273, 1391
Alw26I	GTCTC	1 cut(s)	1340
AlwI	GGATC	4 cut(s)	316, 653, 666, 1518
Ama87I	CYCGRG	1 cut(s)	1486
AoxI	GGCC	5 cut(s)	7, 49, 210, 424, 1192
ApeKI	GCWGC	7 cut(s)	189, 416, 905, 929, 943, 1040, 1079
ApoI	RAATTY	4 cut(s)	280, 474, 484, 1375
AspLEI	GCGC	1 cut(s)	978
AspS9I	GGNCC	6 cut(s)	8, 269, 365, 863, 1071, 1465
AsuC2I	CCSGG	3 cut(s)	6, 1076, 1323
AsuHPI	GGTGA	6 cut(s)	206, 493, 628, 1420, 1462, 1474
AsuII	TTCGAA	1 cut(s)	938
AvaI	CYCGRG	1 cut(s)	1486
AvaII	GGWCC	5 cut(s)	269, 365, 863, 1071, 1465
BaeI	ACNNNNGTAYC	2 cut(s)	503, 536
BalI	TGGCCA	1 cut(s)	51
BamHI	GGATCC	1 cut(s)	658
BanI	GGYRCC	1 cut(s)	1325
BanII	GRGCYC	2 cut(s)	541, 1625
BauI	CACGAG	1 cut(s)	132
BbsI	GAAGAC	1 cut(s)	975
BbvCI	CCTCAGC	2 cut(s)	349, 1036
BbvI	GCAGC	7 cut(s)	176, 403, 892, 916, 930, 1052, 1066
BceAI	ACGGC	1 cut(s)	810
BciT130I	CCWGG	5 cut(s)	290, 912, 1133, 1191, 1361
BciVI	GTATCC	1 cut(s)	531
BcnI	CCSGG	3 cut(s)	6, 1076, 1323
BcoDI	GTCTC	1 cut(s)	1340
BfaI	CTAG	6 cut(s)	30, 449, 926, 1196, 1380, 1589
BfmI	CTRYAG	1 cut(s)	1636
BfrI	CTTAAG	1 cut(s)	773
BfuI	GTATCC	1 cut(s)	531
BglI	GCCNNNNNGGC	1 cut(s)	111
BisI	GCNGC	8 cut(s)	190, 213, 417, 906, 930, 944, 1041, 1080
BlsI	GCNGC	8 cut(s)	191, 214, 418, 907, 931, 945, 1042, 1081
Bme1390I	CCNGG	8 cut(s)	6, 290, 912, 1076, 1133, 1191, 1323, 1361
Bme18I	GGWCC	5 cut(s)	269, 365, 863, 1071, 1465
BmeRI	GACNNNNNGTC	1 cut(s)	1572
BmeT110I	CYCGRG	1 cut(s)	1486
BmgT120I	GGNCC	6 cut(s)	8, 269, 365, 863, 1071, 1465
BmiI	GGNNCC	8 cut(s)	10, 100, 538, 660, 788, 1073, 1327, 1622
BmrFI	CCNGG	8 cut(s)	6, 290, 912, 1076, 1133, 1191, 1323, 1361
BmrI	ACTGGG	1 cut(s)	6
BmsI	GCATC	4 cut(s)	383, 820, 1195, 1522
BmuI	ACTGGG	1 cut(s)	6
BpiI	GAAGAC	1 cut(s)	975
BpmI	CTGGAG	1 cut(s)	272
Bpu10I	CCTNAGC	2 cut(s)	349, 1036
Bpu14I	TTCGAA	1 cut(s)	938
BpuEI	CTTGAG	1 cut(s)	875
BpuMI	CCSGG	3 cut(s)	6, 1076, 1323
BsaAI	YACGTR	2 cut(s)	155, 165
BsaBI	GATNNNNATC	1 cut(s)	254
BsaI	GGTCTC	1 cut(s)	1340
BsaJI	CCNNGG	6 cut(s)	52, 94, 398, 911, 1074, 1131
BsaXI	ACNNNNNCTCC	2 cut(s)	53, 83
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 354, 613, 1136, 1322, 1405
Bse1I	ACTGG	5 cut(s)	12, 35, 187, 427, 1073
Bse3DI	GCAATG	4 cut(s)	114, 228, 297, 840
Bse8I	GATNNNNATC	1 cut(s)	254
BseBI	CCWGG	5 cut(s)	290, 912, 1133, 1191, 1361
BseDI	CCNNGG	6 cut(s)	52, 94, 398, 911, 1074, 1131
BseGI	GGATG	7 cut(s)	374, 738, 835, 885, 1007, 1186, 1603
BseJI	GATNNNNATC	1 cut(s)	254
BseLI	CCNNNNNNNGG	6 cut(s)	95, 354, 613, 1136, 1322, 1405
BseMI	GCAATG	4 cut(s)	114, 228, 297, 840
BseMII	CTCAG	5 cut(s)	363, 623, 1014, 1050, 1317
BseNI	ACTGG	5 cut(s)	12, 35, 187, 427, 1073
BseRI	GAGGAG	1 cut(s)	111
BseXI	GCAGC	7 cut(s)	176, 403, 892, 916, 930, 1052, 1066
BseYI	CCCAGC	2 cut(s)	353, 742
BsgI	GTGCAG	1 cut(s)	891
Bsh1236I	CGCG	1 cut(s)	215
BshFI	GGCC	5 cut(s)	9, 51, 212, 426, 1194
BshNI	GGYRCC	1 cut(s)	1325
BsiHKCI	CYCGRG	1 cut(s)	1486
BsiSI	CCGG	3 cut(s)	6, 1076, 1323
BslFI	GGGAC	3 cut(s)	139, 610, 1057
BslI	CCNNNNNNNGG	6 cut(s)	95, 354, 613, 1136, 1322, 1405
BsmAI	GTCTC	1 cut(s)	1340
BsmFI	GGGAC	3 cut(s)	139, 610, 1057
BsnI	GGCC	5 cut(s)	9, 51, 212, 426, 1194
Bso31I	GGTCTC	1 cut(s)	1340
BsoBI	CYCGRG	1 cut(s)	1486
Bsp119I	TTCGAA	1 cut(s)	938
Bsp1286I	GDGCHC	2 cut(s)	541, 1625
Bsp1407I	TGTACA	1 cut(s)	1437
Bsp143I	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
Bsp19I	CCATGG	2 cut(s)	52, 398
BspACI	CCGC	8 cut(s)	192, 213, 267, 436, 784, 808, 1107, 1317
BspANI	GGCC	5 cut(s)	9, 51, 212, 426, 1194
BspCNI	CTCAG	5 cut(s)	362, 622, 1013, 1049, 1316
BspFNI	CGCG	1 cut(s)	215
BspHI	TCATGA	1 cut(s)	1098
BspLI	GGNNCC	8 cut(s)	10, 100, 538, 660, 788, 1073, 1327, 1622
BspPI	GGATC	4 cut(s)	316, 653, 666, 1518
BspT104I	TTCGAA	1 cut(s)	938
BspT107I	GGYRCC	1 cut(s)	1325
BspTI	CTTAAG	1 cut(s)	773
BspTNI	GGTCTC	1 cut(s)	1340
BsrBI	CCGCTC	1 cut(s)	784
BsrDI	GCAATG	4 cut(s)	114, 228, 297, 840
BsrGI	TGTACA	1 cut(s)	1437
BsrI	ACTGG	5 cut(s)	12, 35, 187, 427, 1073
BssECI	CCNNGG	6 cut(s)	52, 94, 398, 911, 1074, 1131
BssMI	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
BssSI	CACGAG	1 cut(s)	132
BssT1I	CCWWGG	2 cut(s)	52, 398
Bst2BI	CACGAG	1 cut(s)	132
Bst2UI	CCWGG	5 cut(s)	290, 912, 1133, 1191, 1361
Bst4CI	ACNGT	6 cut(s)	412, 593, 725, 820, 1427, 1534
Bst6I	CTCTTC	2 cut(s)	320, 859
BstAFI	CTTAAG	1 cut(s)	773
BstAUI	TGTACA	1 cut(s)	1437
BstBAI	YACGTR	2 cut(s)	155, 165
BstBI	TTCGAA	1 cut(s)	938
BstC8I	GCNNGC	3 cut(s)	103, 175, 1317
BstDEI	CTNAG	7 cut(s)	349, 609, 1000, 1036, 1303, 1400, 1506
BstDSI	CCRYGG	2 cut(s)	52, 398
BstF5I	GGATG	7 cut(s)	374, 738, 835, 885, 1007, 1186, 1603
BstFNI	CGCG	1 cut(s)	215
BstHHI	GCGC	1 cut(s)	978
BstKTI	GATC	6 cut(s)	311, 661, 780, 901, 1406, 1526
BstMAI	GTCTC	1 cut(s)	1340
BstMBI	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
BstMWI	GCNNNNNNNGC	7 cut(s)	111, 1040, 1154, 1236, 1245, 1254, 1519
BstNI	CCWGG	5 cut(s)	290, 912, 1133, 1191, 1361
BstNSI	RCATGY	3 cut(s)	177, 976, 1418
BstSCI	CCNGG	8 cut(s)	4, 288, 910, 1074, 1131, 1189, 1321, 1359
BstSFI	CTRYAG	1 cut(s)	1636
BstUI	CGCG	1 cut(s)	215
BstV1I	GCAGC	7 cut(s)	176, 403, 892, 916, 930, 1052, 1066
BstV2I	GAAGAC	1 cut(s)	975
BstX2I	RGATCY	1 cut(s)	658
BstYI	RGATCY	1 cut(s)	658
BsuI	GTATCC	1 cut(s)	531
BsuRI	GGCC	5 cut(s)	9, 51, 212, 426, 1194
BtgI	CCRYGG	2 cut(s)	52, 398
BtsCI	GGATG	7 cut(s)	374, 738, 835, 885, 1007, 1186, 1603
BtsI	GCAGTG	1 cut(s)	1188
BtsIMutI	CAGTG	3 cut(s)	42, 598, 1188
Cac8I	GCNNGC	3 cut(s)	103, 175, 1317
CciI	TCATGA	1 cut(s)	1098
CfoI	GCGC	1 cut(s)	978
Cfr13I	GGNCC	6 cut(s)	8, 269, 365, 863, 1071, 1465
CsiI	ACCWGGT	1 cut(s)	1359
Csp6I	GTAC	6 cut(s)	86, 166, 1005, 1294, 1423, 1438
CviQI	GTAC	6 cut(s)	86, 166, 1005, 1294, 1423, 1438
DdeI	CTNAG	7 cut(s)	349, 609, 1000, 1036, 1303, 1400, 1506
DpnI	GATC	6 cut(s)	310, 660, 779, 900, 1405, 1525
DpnII	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
DraI	TTTAAA	1 cut(s)	603
DriI	GACNNNNNGTC	1 cut(s)	1572
EaeI	YGGCCR	2 cut(s)	49, 210
Eam1104I	CTCTTC	2 cut(s)	320, 859
Eam1105I	GACNNNNNGTC	1 cut(s)	1572
EarI	CTCTTC	2 cut(s)	320, 859
Eco130I	CCWWGG	2 cut(s)	52, 398
Eco24I	GRGCYC	2 cut(s)	541, 1625
Eco31I	GGTCTC	1 cut(s)	1340
Eco32I	GATATC	1 cut(s)	252
Eco47I	GGWCC	5 cut(s)	269, 365, 863, 1071, 1465
Eco57I	CTGAAG	1 cut(s)	543
Eco88I	CYCGRG	1 cut(s)	1486
EcoO109I	RGGNCCY	2 cut(s)	863, 1465
EcoRI	GAATTC	1 cut(s)	1375
EcoRII	CCWGG	5 cut(s)	288, 910, 1131, 1189, 1359
EcoRV	GATATC	1 cut(s)	252
EcoT14I	CCWWGG	2 cut(s)	52, 398
EcoT22I	ATGCAT	1 cut(s)	376
EcoT38I	GRGCYC	2 cut(s)	541, 1625
ErhI	CCWWGG	2 cut(s)	52, 398
FalI	AAGNNNNNCTT	2 cut(s)	1511, 1543
FaqI	GGGAC	3 cut(s)	139, 610, 1057
FauI	CCCGC	1 cut(s)	1324
FauNDI	CATATG	1 cut(s)	825
FblI	GTMKAC	2 cut(s)	1200, 1635
Fnu4HI	GCNGC	8 cut(s)	190, 213, 417, 906, 930, 944, 1041, 1080
FokI	GGATG	7 cut(s)	361, 725, 842, 872, 994, 1173, 1610
FriOI	GRGCYC	2 cut(s)	541, 1625
Fsp4HI	GCNGC	8 cut(s)	190, 213, 417, 906, 930, 944, 1041, 1080
FspBI	CTAG	6 cut(s)	30, 449, 926, 1196, 1380, 1589
GlaI	GCGC	1 cut(s)	977
GluI	GCNGC	8 cut(s)	190, 213, 417, 906, 930, 944, 1041, 1080
GsaI	CCCAGC	2 cut(s)	357, 746
GsuI	CTGGAG	1 cut(s)	272
HaeIII	GGCC	5 cut(s)	9, 51, 212, 426, 1194
HapII	CCGG	3 cut(s)	6, 1076, 1323
HhaI	GCGC	1 cut(s)	978
Hin6I	GCGC	1 cut(s)	976
HinP1I	GCGC	1 cut(s)	976
HincII	GTYRAC	1 cut(s)	40
HindII	GTYRAC	1 cut(s)	40
HindIII	AAGCTT	1 cut(s)	1271
HinfI	GANTC	4 cut(s)	293, 445, 1479, 1573
HpaII	CCGG	3 cut(s)	6, 1076, 1323
HphI	GGTGA	6 cut(s)	206, 493, 628, 1420, 1462, 1474
Hpy166II	GTNNAC	6 cut(s)	40, 86, 244, 1201, 1438, 1636
Hpy188I	TCNGA	9 cut(s)	95, 308, 387, 562, 612, 1154, 1306, 1374, 1518
Hpy188III	TCNNGA	4 cut(s)	480, 656, 858, 1099
Hpy8I	GTNNAC	6 cut(s)	40, 86, 244, 1201, 1438, 1636
HpyCH4III	ACNGT	6 cut(s)	412, 593, 725, 820, 1427, 1534
HpyCH4IV	ACGT	3 cut(s)	154, 164, 552
HpyCH4V	TGCA	9 cut(s)	119, 374, 395, 639, 908, 932, 1028, 1186, 1239
HpyF10VI	GCNNNNNNNGC	7 cut(s)	111, 1040, 1154, 1236, 1245, 1254, 1519
HpyF3I	CTNAG	7 cut(s)	349, 609, 1000, 1036, 1303, 1400, 1506
HpySE526I	ACGT	3 cut(s)	154, 164, 552
HspAI	GCGC	1 cut(s)	976
Kzo9I	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
LmnI	GCTCC	4 cut(s)	98, 536, 1154, 1620
Lsp1109I	GCAGC	7 cut(s)	176, 403, 892, 916, 930, 1052, 1066
LweI	GCATC	4 cut(s)	383, 820, 1195, 1522
MabI	ACCWGGT	1 cut(s)	1359
MaeI	CTAG	6 cut(s)	30, 449, 926, 1196, 1380, 1589
MaeII	ACGT	3 cut(s)	154, 164, 552
MaeIII	GTNAC	9 cut(s)	14, 150, 194, 553, 587, 725, 1450, 1480, 1490
MalI	GATC	6 cut(s)	310, 660, 779, 900, 1405, 1525
MbiI	CCGCTC	1 cut(s)	784
MboI	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
MboII	GAAGA	4 cut(s)	337, 716, 846, 980
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	2 cut(s)	541, 1625
MlsI	TGGCCA	1 cut(s)	51
MluCI	AATT	4 cut(s)	280, 474, 484, 1375
MluNI	TGGCCA	1 cut(s)	51
MlyI	GAGTC	3 cut(s)	439, 1488, 1582
MmeI	TCCRAC	2 cut(s)	573, 686
Mox20I	TGGCCA	1 cut(s)	51
Mph1103I	ATGCAT	1 cut(s)	376
MscI	TGGCCA	1 cut(s)	51
MseI	TTAA	9 cut(s)	440, 453, 602, 669, 677, 762, 774, 953, 1311
MslI	CAYNNNNRTG	3 cut(s)	828, 1211, 1535
Msp20I	TGGCCA	1 cut(s)	51
MspA1I	CMGCKG	3 cut(s)	353, 808, 1391
MspCI	CTTAAG	1 cut(s)	773
MspI	CCGG	3 cut(s)	6, 1076, 1323
MspR9I	CCNGG	8 cut(s)	6, 290, 912, 1076, 1133, 1191, 1323, 1361
MvaI	CCWGG	5 cut(s)	290, 912, 1133, 1191, 1361
MvnI	CGCG	1 cut(s)	215
MwoI	GCNNNNNNNGC	7 cut(s)	111, 1040, 1154, 1236, 1245, 1254, 1519
NciI	CCSGG	3 cut(s)	6, 1076, 1323
NcoI	CCATGG	2 cut(s)	52, 398
NdeI	CATATG	1 cut(s)	825
NdeII	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
NlaIV	GGNNCC	8 cut(s)	10, 100, 538, 660, 788, 1073, 1327, 1622
NmuCI	GTSAC	5 cut(s)	150, 194, 587, 1450, 1480
NsiI	ATGCAT	1 cut(s)	376
NspI	RCATGY	3 cut(s)	177, 976, 1418
NspV	TTCGAA	1 cut(s)	938
OliI	CACNNNNGTG	1 cut(s)	1535
PaeI	GCATGC	1 cut(s)	177
PagI	TCATGA	1 cut(s)	1098
PfeI	GAWTC	1 cut(s)	293
PfoI	TCCNGGA	1 cut(s)	288
PkrI	GCNGC	8 cut(s)	191, 214, 418, 907, 931, 945, 1042, 1081
PleI	GAGTC	3 cut(s)	439, 1487, 1581
PpsI	GAGTC	3 cut(s)	439, 1487, 1581
Ppu21I	YACGTR	2 cut(s)	155, 165
PpuMI	RGGWCCY	2 cut(s)	863, 1465
Psp1406I	AACGTT	1 cut(s)	552
Psp5II	RGGWCCY	2 cut(s)	863, 1465
Psp6I	CCWGG	5 cut(s)	288, 910, 1131, 1189, 1359
PspFI	CCCAGC	2 cut(s)	353, 742
PspGI	CCWGG	5 cut(s)	288, 910, 1131, 1189, 1359
PspN4I	GGNNCC	8 cut(s)	10, 100, 538, 660, 788, 1073, 1327, 1622
PspPI	GGNCC	6 cut(s)	8, 269, 365, 863, 1071, 1465
PspPPI	RGGWCCY	2 cut(s)	863, 1465
PsuI	RGATCY	1 cut(s)	658
PvuII	CAGCTG	2 cut(s)	353, 1391
RsaI	GTAC	6 cut(s)	87, 167, 1006, 1295, 1424, 1439
RsaNI	GTAC	6 cut(s)	86, 166, 1005, 1294, 1423, 1438
RseI	CAYNNNNRTG	3 cut(s)	828, 1211, 1535
SaqAI	TTAA	9 cut(s)	440, 453, 602, 669, 677, 762, 774, 953, 1311
SatI	GCNGC	8 cut(s)	190, 213, 417, 906, 930, 944, 1041, 1080
Sau3AI	GATC	6 cut(s)	308, 658, 777, 898, 1403, 1523
Sau96I	GGNCC	6 cut(s)	8, 269, 365, 863, 1071, 1465
SchI	GAGTC	3 cut(s)	439, 1488, 1582
ScrFI	CCNGG	8 cut(s)	6, 290, 912, 1076, 1133, 1191, 1323, 1361
SduI	GDGCHC	2 cut(s)	541, 1625
SexAI	ACCWGGT	1 cut(s)	1359
SfaNI	GCATC	4 cut(s)	383, 820, 1195, 1522
SfcI	CTRYAG	1 cut(s)	1636
SfuI	TTCGAA	1 cut(s)	938
SinI	GGWCC	5 cut(s)	269, 365, 863, 1071, 1465
SmiMI	CAYNNNNRTG	3 cut(s)	828, 1211, 1535
SmlI	CTYRAG	2 cut(s)	773, 890
SmoI	CTYRAG	2 cut(s)	773, 890
SphI	GCATGC	1 cut(s)	177
Sse9I	AATT	4 cut(s)	280, 474, 484, 1375
SsiI	CCGC	8 cut(s)	192, 213, 267, 436, 784, 808, 1107, 1317
SspMI	CTAG	6 cut(s)	30, 449, 926, 1196, 1380, 1589
StyD4I	CCNGG	8 cut(s)	4, 288, 910, 1074, 1131, 1189, 1321, 1359
StyI	CCWWGG	2 cut(s)	52, 398
TaaI	ACNGT	6 cut(s)	412, 593, 725, 820, 1427, 1534
TaiI	ACGT	3 cut(s)	157, 167, 555
TaqI	TCGA	2 cut(s)	780, 938
TasI	AATT	4 cut(s)	280, 474, 484, 1375
TatI	WGTACW	2 cut(s)	1293, 1437
TauI	GCSGC	1 cut(s)	215
TfiI	GAWTC	1 cut(s)	293
Tru1I	TTAA	9 cut(s)	440, 453, 602, 669, 677, 762, 774, 953, 1311
Tru9I	TTAA	9 cut(s)	440, 453, 602, 669, 677, 762, 774, 953, 1311
TscAI	CASTG	3 cut(s)	49, 598, 1188
TseFI	GTSAC	5 cut(s)	150, 194, 587, 1450, 1480
TseI	GCWGC	7 cut(s)	189, 416, 905, 929, 943, 1040, 1079
Tsp45I	GTSAC	5 cut(s)	150, 194, 587, 1450, 1480
TspDTI	ATGAA	9 cut(s)	215, 237, 305, 338, 723, 812, 829, 1229, 1571
TspGWI	ACGGA	3 cut(s)	250, 292, 585
TspRI	CASTG	3 cut(s)	49, 598, 1188
Vha464I	CTTAAG	1 cut(s)	773
VpaK11BI	GGWCC	5 cut(s)	269, 365, 863, 1071, 1465
XapI	RAATTY	4 cut(s)	280, 474, 484, 1375
XceI	RCATGY	3 cut(s)	177, 976, 1418
XmiI	GTMKAC	2 cut(s)	1200, 1635
XspI	CTAG	6 cut(s)	30, 449, 926, 1196, 1380, 1589
Zsp2I	ATGCAT	1 cut(s)	376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.