Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0006498

Physical Location & Seq

Genomic Coordinates Reverse (-)

18405536 .. 18409372

Gene Structure 3837 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG205700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGTGGATCTTACGATATCTAGTGCTAGTATTGGTATCCAGTACTTGTGTTGTAGCTACATTGGCTCAAGGAACAAACGAGGATGGTTCTGGCGTTAGGAAGCCTTACATTGTGTACATGGGAGAACTGCCGGAACCCGGATCCTCCACCATGGTCATGACCACAGCCGGCCATGATAACTTGCTCAAGACAGCAATTGGAGATGCCGACTTAGCCAGAGTATCCAAAATTTATACTTATGGAAGGAGCTTCAATGGCTTCGTAGCCAGATTGTTGCCGCATGAAGCAAAGAAACTATCAGATGATGATCGGGTTGTTTCAGTGTTTCCAAACACCAAGCTCAAATTACATACAACAAGGTCATGGGATTTCCTAGGATTGCCAGGAAATACGAAGAAACGAAACTTGCCAATAGAGAGCAAGATCGTAGTGGGTGTGATAGATACAGGAATTTGGCCGGCTTGTCCCAGTATGAACGATTCAGGCTTCGGACCACCCCCAAGAAAATGGAAAGGAAAATGTATGAAGGGCGGTAACTTCACCGGCTGTAACAACAAGGTAATTGGTGCCAAATACTTCATGGTGGACGACATGCCCGGCCCTGGTTATGAGGACAACGCTAGTCCGGTTGACACTGATGGTCATGGCACTCACACAGCCTCCATTGTTGGTGGTGTGCCCGTCCGAGGCGCCAGCCTATATGGCATTGCAAAAGGGACTGCTCGTGGGGGAGCGCCATCGTCACGTATAGCCACGTACAAAGCATGCTGGACTGGTGGCTGCGGTGACATGAACATCTTGGCCGCGTTTGATGAAGCCATTGCTGATGGTGTGGACTTGATATCAATCTCCATTGGCGGTCCTTCACGGAATTTCCTCCAGGATTCCATTGCTCTCGGATCGTTTCATGCCATGAAGAGAGGGATTTTGGTTTCAGCCTCAGCTGGGAATGATGGACCTTATGCATCCTCTGTTCAGAATGTTGCACCATGGATAATGACTGTGGCTGCTACTGGCCTTGATAGGCGGTTAAAGACTCTAGTTAAGTTGGGCAATGGCAAGAAAATTTCTGGAAATTCAATCAATACCTTCACCCCCAAGAAACAGATGTATCCCCTTACAAGTGGAGCCCGTGCTTCCAACGTTACTTCAGACCTCTACGGAAATGCAAGTGCTTGTGACACTGGGACTTTAAACCCTCAGAAGGTGAAGGGAAGGATTGTGTTGTGCAAAGGGCAAAGTAATCAGGATCCAACTATTAAAGACTTAAATGGGGCAGGAACTATTATGACAGAAGATGAAATGCTTGATTACGGTTACACCCCTATCATCCCAGCTACCTATGTGCTTGTTAATGATGGACTTAAGATTGAGCGGTATATAAACACTACCAAGAACCCTACGGCTGTAATACAGCGGACAAGAACTGTTCATATGGATGCCCCCTTCATTGCTTCTTTCTCTTCCAGAGGACCTCAACTCATAACCCCCAACATCCTCAAGCCTGATCTTGCTGCACCAGGGATAAACATACTAGCTGCATATTCGAAGCTGCCATCTTTAACAGGTAGCCTAGAAGACATGCGCCACTATTCGTTCAACATACTCTCAGGTACATCCATGTCTTGCCCTCATGCAACTGCCTCAGCAGCCTATGTCAAGTCCTTCCACCCAGACTGGTCCCCGGCTGCCATCAAGTCGGCTCTCATGACAACCGCCACACCATTGAAAATCAAACCCCTGGAAGGCGAGTTAGCCTTCGGAGCAGGACAAATACAGCCAATAAAGGCAGTGCATCCTGGCCTAGTCTACGACATTTCCATGAACGACTACATCAGCTTCTTGTGCAAGGAAGGCTACAACAGCACCAGCATTGGAAAGCTTATTGGTGGTAAAAAGAAGTACAACTGCTCAGACTTTAAACCCGCACCCGGCACCGATGGCCTCAACTATCCTTCAATGTCCTACCTGGTGCGAAATCCGAATTCTAGCATTTCAGCTGTTTTCCTTAGATCGGTGAAGCATGTAGGGTACGGTAAGTCGGTGTACAAGGCAAAGGTGACTTCGCCAAAGGGCCTTTCGATTAGAGTCACCCCGAGTACCTTGACATTCACTAAGAAGCATCAGAAGCGATCCTTCACGGTTGTGGTGAAGGGTGGCACGATGAAAGGAAAGACAGAGAGTCTGTCTGCTCTACTAGAGTGGGATGATGGTAGGCATACGGTTAGGAGCCCCATCGTAGTCTACAGGCATACCACATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

754

Amino Acids

80.72

Weight (kDa)

9.52

Isoelectric Point (pI)

30.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	36 - 117	1.3e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	140 - 588	1.7e-46	Subtilase family
fn3_6	PF17766	649 - 748	1.1e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006498)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03620 AT5G03620
malus_domestica	MD12G1022400.v1.1 MD12G1022500.v1.1 MD14G1019300.v1.1 MD14G1019400.v1.1
prunus_persica	Prupe.7G114300_v2.0.a1 Prupe.7G114300_v2.0.a1
pyrus_communis	pycom14g01900
rosa_chinensis	RchiOBHm_Chr3g0472671 RchiOBHm_Chr3g0472681
rosa_laevigata	RLG00000016130 RLG00000024064
rosa_multiflora	Rmu_sc0001616.1_g000054 Rmu_sc0003510.1_g000013
rosa_roxburghii	Rroxscaffold_5G00353850 Rroxscaffold_6G00408720 Rroxscaffold_6G00408730
rosa_rugosa	Rorug03G0127100 Rorug04G0092600
rosa_samantha	Rh3AG179800 Rh3BG205700 Rh3CG202800 Rh3DG170000 Rh3DG170100
rosa_wichuraiana	Rw3G016290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	566, 689, 1934
AccBSI	CCGCTC	1 cut(s)	1375
AccI	GTMKAC	2 cut(s)	1809, 2244
AccII	CGCG	1 cut(s)	806
AclI	AACGTT	1 cut(s)	1143
AclWI	GGATC	7 cut(s)	14, 135, 148, 907, 1244, 1257, 2127
AcoI	YGGCCR	3 cut(s)	169, 455, 801
AcsI	RAATTY	6 cut(s)	228, 450, 871, 1065, 1075, 1984
AcuI	CTGAAG	1 cut(s)	1134
AcyI	GRCGYC	1 cut(s)	690
AfaI	GTAC	8 cut(s)	43, 116, 758, 1615, 1904, 2033, 2048, 2101
AfiI	CCNNNNNNNGG	8 cut(s)	137, 602, 686, 945, 1204, 1745, 1931, 2014
AflII	CTTAAG	1 cut(s)	1364
AgsI	TTSAA	5 cut(s)	253, 1080, 1600, 1729, 1959
AhdI	GACNNNNNGTC	1 cut(s)	2181
AjnI	CCWGG	7 cut(s)	382, 601, 879, 1519, 1740, 1798, 1968
AjuI	GAANNNNNNNTTGG	2 cut(s)	2062, 2094
AleI	CACNNNNGTG	1 cut(s)	2144
AlwI	GGATC	7 cut(s)	14, 135, 148, 907, 1244, 1257, 2127
Ama87I	CYCGRG	1 cut(s)	2095
AoxI	GGCC	8 cut(s)	169, 455, 598, 801, 1015, 1801, 1942, 2074
ApeKI	GCWGC	7 cut(s)	780, 1007, 1514, 1538, 1552, 1649, 1688
ApoI	RAATTY	6 cut(s)	228, 450, 871, 1065, 1075, 1984
AspA2I	CCTAGG	1 cut(s)	373
AspLEI	GCGC	3 cut(s)	692, 736, 1587
AspS9I	GGNCC	7 cut(s)	491, 599, 860, 956, 1472, 1680, 2074
AsuC2I	CCSGG	4 cut(s)	138, 597, 1685, 1932
AsuHPI	GGTGA	8 cut(s)	532, 797, 1084, 1219, 2029, 2071, 2083, 2161
AsuII	TTCGAA	1 cut(s)	1547
AvaI	CYCGRG	1 cut(s)	2095
AvaII	GGWCC	5 cut(s)	491, 860, 956, 1472, 1680
AvrII	CCTAGG	1 cut(s)	373
BaeGI	GKGCMC	1 cut(s)	681
BaeI	ACNNNNGTAYC	2 cut(s)	1094, 1127
BamHI	GGATCC	2 cut(s)	140, 1249
BanI	GGYRCC	3 cut(s)	566, 689, 1934
BanII	GRGCYC	2 cut(s)	1132, 2234
BauI	CACGAG	1 cut(s)	723
BbsI	GAAGAC	1 cut(s)	1584
BbvCI	CCTCAGC	2 cut(s)	940, 1645
BbvI	GCAGC	7 cut(s)	767, 994, 1501, 1525, 1539, 1661, 1675
BceAI	ACGGC	1 cut(s)	1419
BciT130I	CCWGG	7 cut(s)	384, 603, 881, 1521, 1742, 1800, 1970
BciVI	GTATCC	3 cut(s)	46, 232, 1122
BcnI	CCSGG	4 cut(s)	138, 597, 1685, 1932
BfmI	CTRYAG	1 cut(s)	2245
BfoI	RGCGCY	2 cut(s)	693, 737
BfrI	CTTAAG	1 cut(s)	1364
BfuI	GTATCC	3 cut(s)	46, 232, 1122
BglI	GCCNNNNNGGC	1 cut(s)	702
BisI	GCNGC	9 cut(s)	278, 781, 804, 1008, 1515, 1539, 1553, 1650, 1689
BlnI	CCTAGG	1 cut(s)	373
BlsI	GCNGC	9 cut(s)	279, 782, 805, 1009, 1516, 1540, 1554, 1651, 1690
BmcAI	AGTACT	1 cut(s)	43
Bme18I	GGWCC	5 cut(s)	491, 860, 956, 1472, 1680
BmeRI	GACNNNNNGTC	1 cut(s)	2181
BmeT110I	CYCGRG	1 cut(s)	2095
BmgT120I	GGNCC	7 cut(s)	491, 599, 860, 956, 1472, 1680, 2074
BmiI	GGNNCC	9 cut(s)	135, 142, 568, 691, 1129, 1251, 1682, 1936, 2231
BmrI	ACTGGG	2 cut(s)	462, 1194
BmsI	GCATC	5 cut(s)	193, 974, 1429, 1804, 2131
BmuI	ACTGGG	2 cut(s)	462, 1194
BpiI	GAAGAC	1 cut(s)	1584
BpmI	CTGGAG	1 cut(s)	863
Bpu10I	CCTNAGC	2 cut(s)	940, 1645
Bpu14I	TTCGAA	1 cut(s)	1547
BpuEI	CTTGAG	3 cut(s)	51, 170, 1484
BpuMI	CCSGG	4 cut(s)	138, 597, 1685, 1932
BsaAI	YACGTR	2 cut(s)	746, 756
BsaBI	GATNNNNATC	2 cut(s)	306, 845
BsaHI	GRCGYC	1 cut(s)	690
BsaJI	CCNNGG	8 cut(s)	150, 373, 601, 685, 989, 1520, 1683, 1740
BsaWI	WCCGGW	1 cut(s)	625
BsaXI	ACNNNNNCTCC	2 cut(s)	644, 674
Bsc4I	CCNNNNNNNGG	8 cut(s)	137, 602, 686, 945, 1204, 1745, 1931, 2014
Bse118I	RCCGGY	3 cut(s)	167, 457, 542
Bse1I	ACTGG	6 cut(s)	39, 468, 778, 1018, 1189, 1682
Bse3DI	GCAATG	5 cut(s)	705, 819, 888, 1060, 1449
Bse8I	GATNNNNATC	2 cut(s)	306, 845
BseBI	CCWGG	7 cut(s)	384, 603, 881, 1521, 1742, 1800, 1970
BseDI	CCNNGG	8 cut(s)	150, 373, 601, 685, 989, 1520, 1683, 1740
BseGI	GGATG	8 cut(s)	88, 965, 1329, 1444, 1494, 1616, 1795, 2212
BseJI	GATNNNNATC	2 cut(s)	306, 845
BseLI	CCNNNNNNNGG	8 cut(s)	137, 602, 686, 945, 1204, 1745, 1931, 2014
BseMI	GCAATG	5 cut(s)	705, 819, 888, 1060, 1449
BseMII	CTCAG	5 cut(s)	954, 1214, 1623, 1659, 1926
BseNI	ACTGG	6 cut(s)	39, 468, 778, 1018, 1189, 1682
BseSI	GKGCMC	1 cut(s)	681
BseXI	GCAGC	7 cut(s)	767, 994, 1501, 1525, 1539, 1661, 1675
BseYI	CCCAGC	2 cut(s)	944, 1333
BsgI	GTGCAG	1 cut(s)	1500
Bsh1236I	CGCG	1 cut(s)	806
BshFI	GGCC	8 cut(s)	171, 457, 600, 803, 1017, 1803, 1944, 2076
BshNI	GGYRCC	3 cut(s)	566, 689, 1934
BsiHKCI	CYCGRG	1 cut(s)	2095
BsiSI	CCGG	9 cut(s)	131, 138, 168, 458, 543, 597, 626, 1685, 1932
BslFI	GGGAC	4 cut(s)	450, 730, 1201, 1666
BslI	CCNNNNNNNGG	8 cut(s)	137, 602, 686, 945, 1204, 1745, 1931, 2014
BsmFI	GGGAC	4 cut(s)	450, 730, 1201, 1666
BsnI	GGCC	8 cut(s)	171, 457, 600, 803, 1017, 1803, 1944, 2076
BsoBI	CYCGRG	1 cut(s)	2095
Bsp119I	TTCGAA	1 cut(s)	1547
Bsp1286I	GDGCHC	3 cut(s)	681, 1132, 2234
Bsp1407I	TGTACA	2 cut(s)	114, 2046
Bsp143I	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
Bsp19I	CCATGG	2 cut(s)	150, 989
BspANI	GGCC	8 cut(s)	171, 457, 600, 803, 1017, 1803, 1944, 2076
BspCNI	CTCAG	5 cut(s)	953, 1213, 1622, 1658, 1925
BspFNI	CGCG	1 cut(s)	806
BspHI	TCATGA	2 cut(s)	156, 1707
BspLI	GGNNCC	9 cut(s)	135, 142, 568, 691, 1129, 1251, 1682, 1936, 2231
BspPI	GGATC	7 cut(s)	14, 135, 148, 907, 1244, 1257, 2127
BspT104I	TTCGAA	1 cut(s)	1547
BspT107I	GGYRCC	3 cut(s)	566, 689, 1934
BspTI	CTTAAG	1 cut(s)	1364
BsrBI	CCGCTC	1 cut(s)	1375
BsrDI	GCAATG	5 cut(s)	705, 819, 888, 1060, 1449
BsrFI	RCCGGY	3 cut(s)	167, 457, 542
BsrGI	TGTACA	2 cut(s)	114, 2046
BsrI	ACTGG	6 cut(s)	39, 468, 778, 1018, 1189, 1682
BssAI	RCCGGY	3 cut(s)	167, 457, 542
BssECI	CCNNGG	8 cut(s)	150, 373, 601, 685, 989, 1520, 1683, 1740
BssMI	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
BssNI	GRCGYC	1 cut(s)	690
BssSI	CACGAG	1 cut(s)	723
BssT1I	CCWWGG	3 cut(s)	150, 373, 989
Bst2BI	CACGAG	1 cut(s)	723
Bst2UI	CCWGG	7 cut(s)	384, 603, 881, 1521, 1742, 1800, 1970
Bst4CI	ACNGT	6 cut(s)	1003, 1316, 1429, 2036, 2143, 2224
Bst6I	CTCTTC	2 cut(s)	911, 1468
BstACI	GRCGYC	1 cut(s)	690
BstAFI	CTTAAG	1 cut(s)	1364
BstAUI	TGTACA	2 cut(s)	114, 2046
BstBAI	YACGTR	2 cut(s)	746, 756
BstBI	TTCGAA	1 cut(s)	1547
BstC8I	GCNNGC	4 cut(s)	169, 459, 694, 766
BstDEI	CTNAG	8 cut(s)	211, 940, 1200, 1609, 1645, 1912, 2009, 2115
BstDSI	CCRYGG	2 cut(s)	150, 989
BstF5I	GGATG	8 cut(s)	88, 965, 1329, 1444, 1494, 1616, 1795, 2212
BstFNI	CGCG	1 cut(s)	806
BstH2I	RGCGCY	2 cut(s)	693, 737
BstHHI	GCGC	3 cut(s)	692, 736, 1587
BstKTI	GATC	9 cut(s)	9, 143, 310, 426, 902, 1252, 1510, 2015, 2135
BstMBI	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
BstNI	CCWGG	7 cut(s)	384, 603, 881, 1521, 1742, 1800, 1970
BstNSI	RCATGY	4 cut(s)	595, 768, 1585, 2027
BstSFI	CTRYAG	1 cut(s)	2245
BstSLI	GKGCMC	1 cut(s)	681
BstUI	CGCG	1 cut(s)	806
BstV1I	GCAGC	7 cut(s)	767, 994, 1501, 1525, 1539, 1661, 1675
BstV2I	GAAGAC	1 cut(s)	1584
BstX2I	RGATCY	3 cut(s)	6, 140, 1249
BstXI	CCANNNNNNTGG	1 cut(s)	507
BstYI	RGATCY	3 cut(s)	6, 140, 1249
BsuI	GTATCC	3 cut(s)	46, 232, 1122
BsuRI	GGCC	8 cut(s)	171, 457, 600, 803, 1017, 1803, 1944, 2076
BtgI	CCRYGG	2 cut(s)	150, 989
BtsCI	GGATG	8 cut(s)	88, 965, 1329, 1444, 1494, 1616, 1795, 2212
BtsI	GCAGTG	1 cut(s)	1797
BtsIMutI	CAGTG	4 cut(s)	327, 633, 1182, 1797
Cac8I	GCNNGC	4 cut(s)	169, 459, 694, 766
CciI	TCATGA	2 cut(s)	156, 1707
CfoI	GCGC	3 cut(s)	692, 736, 1587
Cfr10I	RCCGGY	3 cut(s)	167, 457, 542
Cfr13I	GGNCC	7 cut(s)	491, 599, 860, 956, 1472, 1680, 2074
CsiI	ACCWGGT	1 cut(s)	1968
Csp6I	GTAC	8 cut(s)	42, 115, 757, 1614, 1903, 2032, 2047, 2100
CviQI	GTAC	8 cut(s)	42, 115, 757, 1614, 1903, 2032, 2047, 2100
DdeI	CTNAG	8 cut(s)	211, 940, 1200, 1609, 1645, 1912, 2009, 2115
DinI	GGCGCC	1 cut(s)	691
DpnI	GATC	9 cut(s)	8, 142, 309, 425, 901, 1251, 1509, 2014, 2134
DpnII	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
DraI	TTTAAA	2 cut(s)	1194, 1921
DriI	GACNNNNNGTC	1 cut(s)	2181
EaeI	YGGCCR	3 cut(s)	169, 455, 801
Eam1104I	CTCTTC	2 cut(s)	911, 1468
Eam1105I	GACNNNNNGTC	1 cut(s)	2181
EarI	CTCTTC	2 cut(s)	911, 1468
Eco130I	CCWWGG	3 cut(s)	150, 373, 989
Eco24I	GRGCYC	2 cut(s)	1132, 2234
Eco32I	GATATC	2 cut(s)	17, 843
Eco47I	GGWCC	5 cut(s)	491, 860, 956, 1472, 1680
Eco57I	CTGAAG	1 cut(s)	1134
Eco88I	CYCGRG	1 cut(s)	2095
EcoO109I	RGGNCCY	2 cut(s)	1472, 2074
EcoRI	GAATTC	1 cut(s)	1984
EcoRII	CCWGG	7 cut(s)	382, 601, 879, 1519, 1740, 1798, 1968
EcoRV	GATATC	2 cut(s)	17, 843
EcoT14I	CCWWGG	3 cut(s)	150, 373, 989
EcoT22I	ATGCAT	1 cut(s)	967
EcoT38I	GRGCYC	2 cut(s)	1132, 2234
EgeI	GGCGCC	1 cut(s)	691
EheI	GGCGCC	1 cut(s)	691
ErhI	CCWWGG	3 cut(s)	150, 373, 989
FalI	AAGNNNNNCTT	2 cut(s)	2120, 2152
FaqI	GGGAC	4 cut(s)	450, 730, 1201, 1666
FauI	CCCGC	1 cut(s)	1933
FauNDI	CATATG	1 cut(s)	1434
FblI	GTMKAC	2 cut(s)	1809, 2244
Fnu4HI	GCNGC	9 cut(s)	278, 781, 804, 1008, 1515, 1539, 1553, 1650, 1689
FokI	GGATG	8 cut(s)	95, 952, 1316, 1451, 1481, 1603, 1782, 2219
FriOI	GRGCYC	2 cut(s)	1132, 2234
Fsp4HI	GCNGC	9 cut(s)	278, 781, 804, 1008, 1515, 1539, 1553, 1650, 1689
GlaI	GCGC	3 cut(s)	691, 735, 1586
GluI	GCNGC	9 cut(s)	278, 781, 804, 1008, 1515, 1539, 1553, 1650, 1689
GsaI	CCCAGC	2 cut(s)	948, 1337
GsuI	CTGGAG	1 cut(s)	863
HaeII	RGCGCY	2 cut(s)	693, 737
HaeIII	GGCC	8 cut(s)	171, 457, 600, 803, 1017, 1803, 1944, 2076
HapII	CCGG	9 cut(s)	131, 138, 168, 458, 543, 597, 626, 1685, 1932
HhaI	GCGC	3 cut(s)	692, 736, 1587
Hin1I	GRCGYC	1 cut(s)	690
Hin6I	GCGC	3 cut(s)	690, 734, 1585
HinP1I	GCGC	3 cut(s)	690, 734, 1585
HincII	GTYRAC	1 cut(s)	631
HindII	GTYRAC	1 cut(s)	631
HindIII	AAGCTT	1 cut(s)	1880
HinfI	GANTC	5 cut(s)	479, 884, 1036, 2088, 2182
HpaII	CCGG	9 cut(s)	131, 138, 168, 458, 543, 597, 626, 1685, 1932
HphI	GGTGA	8 cut(s)	532, 797, 1084, 1219, 2029, 2071, 2083, 2161
Hpy166II	GTNNAC	7 cut(s)	115, 586, 631, 835, 1810, 2047, 2245
Hpy188III	TCNNGA	6 cut(s)	157, 187, 1071, 1247, 1467, 1708
Hpy8I	GTNNAC	7 cut(s)	115, 586, 631, 835, 1810, 2047, 2245
HpyCH4III	ACNGT	6 cut(s)	1003, 1316, 1429, 2036, 2143, 2224
HpyCH4IV	ACGT	3 cut(s)	745, 755, 1143
HpyF3I	CTNAG	8 cut(s)	211, 940, 1200, 1609, 1645, 1912, 2009, 2115
HpySE526I	ACGT	3 cut(s)	745, 755, 1143
Hsp92I	GRCGYC	1 cut(s)	690
HspAI	GCGC	3 cut(s)	690, 734, 1585
KasI	GGCGCC	1 cut(s)	689
KroI	GCCGGC	2 cut(s)	167, 457
KroNI	GCCGGC	2 cut(s)	169, 459
Kzo9I	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
LmnI	GCTCC	5 cut(s)	246, 731, 1127, 1763, 2229
Lsp1109I	GCAGC	7 cut(s)	767, 994, 1501, 1525, 1539, 1661, 1675
LweI	GCATC	5 cut(s)	193, 974, 1429, 1804, 2131
MabI	ACCWGGT	1 cut(s)	1968
MaeII	ACGT	3 cut(s)	745, 755, 1143
MaeIII	GTNAC	9 cut(s)	533, 548, 741, 785, 1144, 1178, 1316, 2059, 2089
MalI	GATC	9 cut(s)	8, 142, 309, 425, 901, 1251, 1509, 2014, 2134
MbiI	CCGCTC	1 cut(s)	1375
MboI	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
MboII	GAAGA	5 cut(s)	406, 928, 1307, 1455, 1589
MfeI	CAATTG	1 cut(s)	195
MflI	RGATCY	3 cut(s)	6, 140, 1249
MhlI	GDGCHC	3 cut(s)	681, 1132, 2234
MluCI	AATT	9 cut(s)	195, 228, 344, 450, 561, 871, 1065, 1075, 1984
Mly113I	GGCGCC	1 cut(s)	690
MlyI	GAGTC	3 cut(s)	1030, 2097, 2191
MmeI	TCCRAC	2 cut(s)	1164, 1277
Mph1103I	ATGCAT	1 cut(s)	967
MroNI	GCCGGC	2 cut(s)	167, 457
MseI	TTAA	9 cut(s)	1031, 1044, 1193, 1260, 1268, 1353, 1365, 1562, 1920
MslI	CAYNNNNRTG	4 cut(s)	155, 1437, 1820, 2144
MspA1I	CMGCKG	3 cut(s)	944, 1417, 2000
MspCI	CTTAAG	1 cut(s)	1364
MspI	CCGG	9 cut(s)	131, 138, 168, 458, 543, 597, 626, 1685, 1932
MunI	CAATTG	1 cut(s)	195
MvaI	CCWGG	7 cut(s)	384, 603, 881, 1521, 1742, 1800, 1970
MvnI	CGCG	1 cut(s)	806
NaeI	GCCGGC	2 cut(s)	169, 459
NarI	GGCGCC	1 cut(s)	690
NciI	CCSGG	4 cut(s)	138, 597, 1685, 1932
NcoI	CCATGG	2 cut(s)	150, 989
NdeI	CATATG	1 cut(s)	1434
NdeII	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
NgoMIV	GCCGGC	2 cut(s)	167, 457
NlaIV	GGNNCC	9 cut(s)	135, 142, 568, 691, 1129, 1251, 1682, 1936, 2231
NmuCI	GTSAC	5 cut(s)	741, 785, 1178, 2059, 2089
NsiI	ATGCAT	1 cut(s)	967
NspI	RCATGY	4 cut(s)	595, 768, 1585, 2027
NspV	TTCGAA	1 cut(s)	1547
OliI	CACNNNNGTG	1 cut(s)	2144
PaeI	GCATGC	1 cut(s)	768
PagI	TCATGA	2 cut(s)	156, 1707
PdiI	GCCGGC	2 cut(s)	169, 459
PfeI	GAWTC	2 cut(s)	479, 884
PfoI	TCCNGGA	1 cut(s)	879
PkrI	GCNGC	9 cut(s)	279, 782, 805, 1009, 1516, 1540, 1554, 1651, 1690
PleI	GAGTC	3 cut(s)	1030, 2096, 2190
PluTI	GGCGCC	1 cut(s)	693
PpsI	GAGTC	3 cut(s)	1030, 2096, 2190
Ppu21I	YACGTR	2 cut(s)	746, 756
PpuMI	RGGWCCY	1 cut(s)	1472
Psp1406I	AACGTT	1 cut(s)	1143
Psp5II	RGGWCCY	1 cut(s)	1472
Psp6I	CCWGG	7 cut(s)	382, 601, 879, 1519, 1740, 1798, 1968
PspFI	CCCAGC	2 cut(s)	944, 1333
PspGI	CCWGG	7 cut(s)	382, 601, 879, 1519, 1740, 1798, 1968
PspN4I	GGNNCC	9 cut(s)	135, 142, 568, 691, 1129, 1251, 1682, 1936, 2231
PspPI	GGNCC	7 cut(s)	491, 599, 860, 956, 1472, 1680, 2074
PspPPI	RGGWCCY	1 cut(s)	1472
PsuI	RGATCY	3 cut(s)	6, 140, 1249
PvuII	CAGCTG	2 cut(s)	944, 2000
RsaI	GTAC	8 cut(s)	43, 116, 758, 1615, 1904, 2033, 2048, 2101
RsaNI	GTAC	8 cut(s)	42, 115, 757, 1614, 1903, 2032, 2047, 2100
RseI	CAYNNNNRTG	4 cut(s)	155, 1437, 1820, 2144
SaqAI	TTAA	9 cut(s)	1031, 1044, 1193, 1260, 1268, 1353, 1365, 1562, 1920
SatI	GCNGC	9 cut(s)	278, 781, 804, 1008, 1515, 1539, 1553, 1650, 1689
Sau3AI	GATC	9 cut(s)	6, 140, 307, 423, 899, 1249, 1507, 2012, 2132
Sau96I	GGNCC	7 cut(s)	491, 599, 860, 956, 1472, 1680, 2074
ScaI	AGTACT	1 cut(s)	43
SchI	GAGTC	3 cut(s)	1030, 2097, 2191
SduI	GDGCHC	3 cut(s)	681, 1132, 2234
SexAI	ACCWGGT	1 cut(s)	1968
SfaNI	GCATC	5 cut(s)	193, 974, 1429, 1804, 2131
SfcI	CTRYAG	1 cut(s)	2245
SfoI	GGCGCC	1 cut(s)	691
SfuI	TTCGAA	1 cut(s)	1547
SinI	GGWCC	5 cut(s)	491, 860, 956, 1472, 1680
SmiMI	CAYNNNNRTG	4 cut(s)	155, 1437, 1820, 2144
SmlI	CTYRAG	4 cut(s)	66, 185, 1364, 1499
SmoI	CTYRAG	4 cut(s)	66, 185, 1364, 1499
SphI	GCATGC	1 cut(s)	768
Sse9I	AATT	9 cut(s)	195, 228, 344, 450, 561, 871, 1065, 1075, 1984
SspDI	GGCGCC	1 cut(s)	689
StyI	CCWWGG	3 cut(s)	150, 373, 989
TaaI	ACNGT	6 cut(s)	1003, 1316, 1429, 2036, 2143, 2224
TaiI	ACGT	3 cut(s)	748, 758, 1146
TaqI	TCGA	2 cut(s)	1547, 2081
TasI	AATT	9 cut(s)	195, 228, 344, 450, 561, 871, 1065, 1075, 1984
TatI	WGTACW	4 cut(s)	41, 114, 1902, 2046
TauI	GCSGC	2 cut(s)	280, 806
TfiI	GAWTC	2 cut(s)	479, 884
Tru1I	TTAA	9 cut(s)	1031, 1044, 1193, 1260, 1268, 1353, 1365, 1562, 1920
Tru9I	TTAA	9 cut(s)	1031, 1044, 1193, 1260, 1268, 1353, 1365, 1562, 1920
TscAI	CASTG	4 cut(s)	327, 640, 1189, 1797
TseFI	GTSAC	5 cut(s)	741, 785, 1178, 2059, 2089
TseI	GCWGC	7 cut(s)	780, 1007, 1514, 1538, 1552, 1649, 1688
Tsp45I	GTSAC	5 cut(s)	741, 785, 1178, 2059, 2089
TspGWI	ACGGA	2 cut(s)	883, 1176
TspRI	CASTG	4 cut(s)	327, 640, 1189, 1797
Vha464I	CTTAAG	1 cut(s)	1364
VpaK11BI	GGWCC	5 cut(s)	491, 860, 956, 1472, 1680
XapI	RAATTY	6 cut(s)	228, 450, 871, 1065, 1075, 1984
XceI	RCATGY	4 cut(s)	595, 768, 1585, 2027
XcmI	CCANNNNNNNNNTGG	1 cut(s)	577
XmaJI	CCTAGG	1 cut(s)	373
XmiI	GTMKAC	2 cut(s)	1809, 2244
ZrmI	AGTACT	1 cut(s)	43
Zsp2I	ATGCAT	1 cut(s)	967

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.