Rroxscaffold_6G00408720

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006498
Physical Location & Seq
Forward (+)
31241780 .. 31242309
530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408720.1

Sequence Viewer

Length: 309 bp
ATGACCACAGCCGGCCATGATAACTTGCTCAAGACAGCAATTGGAGATGCCGACTTAGCCAGAGTATCCAAAATTTATACTTATGGAAGGAGCTTCAATGGCTTCGTCGCCAGATTGTTGCCGCATGAAGCAAAGAAATTATCAGATGATGATCGGGTTGTTTCAGTGTTTCCGAACACCAAGCTCAAATTACATACAACAAGCTCATGGGATTTCCTAGGATTGCCAGGAAATACGAAGAAACGAAACTTGCCAATAGAGAGCAAGATCGTAGTGGGTGTGATAGATACAGGTTGGTCCATAGACTAG

Protein Analysis

102

Amino Acids

11.24

Weight (kDa)

9.35

Isoelectric Point (pI)

16.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 6 - 65 3.6e-13 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 72
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 3 cut(s) 93, 184, 204
AluI AGCT 3 cut(s) 93, 184, 204
AoxI GGCC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 72
AspA2I CCTAGG 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 297
AvaII GGWCC 1 cut(s) 297
AvrII CCTAGG 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 228
BciVI GTATCC 1 cut(s) 76
BfaI CTAG 2 cut(s) 218, 307
BfuI GTATCC 1 cut(s) 76
BisI GCNGC 1 cut(s) 122
BlnI CCTAGG 1 cut(s) 217
BlsI GCNGC 1 cut(s) 123
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 11
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 217
BseJI GATNNNNATC 1 cut(s) 150
BshFI GGCC 1 cut(s) 15
BsiSI CCGG 1 cut(s) 12
BsnI GGCC 1 cut(s) 15
Bsp143I GATC 2 cut(s) 151, 267
BspACI CCGC 1 cut(s) 122
BspANI GGCC 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 151, 267
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 55
BstKTI GATC 2 cut(s) 154, 270
BstMBI GATC 2 cut(s) 151, 267
BstMWI GCNNNNNNNGC 2 cut(s) 56, 99
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 226
BsuI GTATCC 1 cut(s) 76
BsuRI GGCC 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 297
CviAII CATG 3 cut(s) 17, 125, 207
CviJI RGCY 7 cut(s) 11, 15, 59, 93, 102, 184, 204
CviKI_1 RGCY 7 cut(s) 11, 15, 59, 93, 102, 184, 204
DdeI CTNAG 1 cut(s) 55
DpnI GATC 2 cut(s) 153, 269
DpnII GATC 2 cut(s) 151, 267
EaeI YGGCCR 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 3 cut(s) 20, 128, 210
FaiI YATR 7 cut(s) 18, 78, 84, 126, 195, 208, 302
FatI CATG 3 cut(s) 16, 124, 206
Fnu4HI GCNGC 1 cut(s) 122
Fsp4HI GCNGC 1 cut(s) 122
FspBI CTAG 2 cut(s) 218, 307
GluI GCNGC 1 cut(s) 122
HaeIII GGCC 1 cut(s) 15
HapII CCGG 1 cut(s) 12
Hin1II CATG 3 cut(s) 20, 128, 210
HpaII CCGG 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 145, 174
Hpy188III TCNNGA 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 99
HpyF3I CTNAG 1 cut(s) 55
Hsp92II CATG 3 cut(s) 20, 128, 210
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Kzo9I GATC 2 cut(s) 151, 267
LmnI GCTCC 1 cut(s) 90
LpnPI CCDG 6 cut(s) 25, 73, 124, 213, 240, 276
LweI GCATC 1 cut(s) 37
MaeI CTAG 2 cut(s) 218, 307
MalI GATC 2 cut(s) 153, 269
MboI GATC 2 cut(s) 151, 267
MboII GAAGA 1 cut(s) 250
MfeI CAATTG 1 cut(s) 39
MluCI AATT 4 cut(s) 39, 72, 137, 188
MroNI GCCGGC 1 cut(s) 11
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 56, 99
NaeI GCCGGC 1 cut(s) 13
NdeII GATC 2 cut(s) 151, 267
NgoMIV GCCGGC 1 cut(s) 11
NlaIII CATG 3 cut(s) 20, 128, 210
PdiI GCCGGC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspPI GGNCC 1 cut(s) 297
SatI GCNGC 1 cut(s) 122
Sau3AI GATC 2 cut(s) 151, 267
Sau96I GGNCC 1 cut(s) 297
ScrFI CCNGG 1 cut(s) 228
SetI ASST 4 cut(s) 95, 186, 206, 295
SfaNI GCATC 1 cut(s) 37
SinI GGWCC 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 4 cut(s) 39, 72, 137, 188
SsiI CCGC 1 cut(s) 122
SspMI CTAG 2 cut(s) 218, 307
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 217
TasI AATT 4 cut(s) 39, 72, 137, 188
TauI GCSGC 1 cut(s) 124
TscAI CASTG 1 cut(s) 171
TspDTI ATGAA 1 cut(s) 141
TspRI CASTG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 1 cut(s) 72
XmaJI CCTAGG 1 cut(s) 217
XspI CTAG 2 cut(s) 218, 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.