Rh3DG170000

Xylem serine proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006498
Physical Location & Seq
Forward (+)
14908491 .. 14911174
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG170000.1

Sequence Viewer

Length: 1101 bp
ATGTGGATCTTACGATATCTAGTGCTAGTATTGGTATCCAGTACTTGTGTTGTAGCTACATTGGCTCAAGGAACAAACGAGGATGGTTCTGGCGTTAGGAAGCCTTACATTGTGTACATGGGAGAACTGCCGGAACCCGGATCCTCCACCATGGTCATGACCACAGCCGGCCATGATAACTTGCTCAAGACAGCAATTGGAGATGCCGACTTAGCCAGAGTATCCAAAATTTATACTTATGGAAGGAGCTTCAATGGCTTCGTAGCCAGATTGTTGCCGCATGAAGCAAAGAAACTATCAGATGATGATCGGGTTGTTTCAGTGTTTCCAAACACCAAGCTCAAATTACATACAACAAGGTCATGGGATTTCCTAGGATTGCCAGGAAATACGAAGAAACGAAACTTGCCAATAGAGAGCAAGATCGTAGTGGGTGTGATAGATACAGGAATTTGGCCGGCTTGTCCCAGTATGAACGATTCAGGCTTCGGACCACCCCCAAGAAAATGGAAAGGAAAATGTATGAAGGGCGGTAACTTCACCGGCTGTAACAACAAGGTAATTGGTGCCAAATACTTCATGGTGGACGACATGCCCGGCCCTGGTTACGAGGACAACGCTAGTCCAGTTGACACTGATGCCTCAGCTGGGAATGATGGACCTTATGCATCTTCTGTTCAGAATGTTGCACCATGGATAATGACTGTGGCTGCTACTGGCCTTGATAGGCGGTTAAAGACTCTAGTTAAGTTGGGCAATGGCAAGAAAATTTCTGGAAATTCAATCAATACCTTCACCCCCAAGAAACAGATGTATCCCCTTACAAGTGGAGCCCGTGCTTCCAACGTTACTTCAGACCTCTACGGAAATGCAAGTGCTTGTGACACTGGGACTTTAAACCCTCAGAAGGTGAAGGGAAGGATTGTGTTGTGCAAAGGGCAAAGTAATCAGGATCCAACTATTAAAGACTTAAATGGGGCAGGAACTATTATGACAGAAGATGAAATGCTTGATTACGGTTACACCCCTATCATCCCAGCTACCTATGTGCTTGTTAATGATGGACTTAAGATTGAGCGGTATATAAACACTACCAAGTAA

Protein Analysis

366

Amino Acids

39.52

Weight (kDa)

9.1

Isoelectric Point (pI)

24.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 36 - 117 4.3e-15 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 1078
AciI CCGC 4 cut(s) 278, 531, 730, 1078
AclI AACGTT 1 cut(s) 846
AclWI GGATC 5 cut(s) 14, 135, 148, 947, 960
AcoI YGGCCR 2 cut(s) 169, 455
AcsI RAATTY 4 cut(s) 228, 450, 768, 778
AcuI CTGAAG 1 cut(s) 837
AfaI GTAC 2 cut(s) 43, 116
AfiI CCNNNNNNNGG 4 cut(s) 137, 602, 648, 907
AflII CTTAAG 1 cut(s) 1067
AgsI TTSAA 2 cut(s) 253, 783
AjnI CCWGG 2 cut(s) 382, 601
AluBI AGCT 5 cut(s) 56, 249, 340, 647, 1040
AluI AGCT 5 cut(s) 56, 249, 340, 647, 1040
AlwI GGATC 5 cut(s) 14, 135, 148, 947, 960
AoxI GGCC 4 cut(s) 169, 455, 598, 718
ApeKI GCWGC 1 cut(s) 710
ApoI RAATTY 4 cut(s) 228, 450, 768, 778
AspA2I CCTAGG 1 cut(s) 373
AspS9I GGNCC 3 cut(s) 491, 599, 659
AsuC2I CCSGG 2 cut(s) 138, 597
AsuHPI GGTGA 3 cut(s) 532, 787, 922
AvaII GGWCC 2 cut(s) 491, 659
AvrII CCTAGG 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 797, 830
BamHI GGATCC 2 cut(s) 140, 952
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 1 cut(s) 835
BbvCI CCTCAGC 1 cut(s) 643
BbvI GCAGC 1 cut(s) 697
BccI CCATC 3 cut(s) 77, 650, 1055
BciT130I CCWGG 2 cut(s) 384, 603
BciVI GTATCC 3 cut(s) 46, 232, 825
BcnI CCSGG 2 cut(s) 138, 597
BfaI CTAG 5 cut(s) 20, 26, 374, 621, 743
BfrI CTTAAG 1 cut(s) 1067
BfuI GTATCC 3 cut(s) 46, 232, 825
BisI GCNGC 2 cut(s) 278, 711
BlnI CCTAGG 1 cut(s) 373
BlsI GCNGC 2 cut(s) 279, 712
BmcAI AGTACT 1 cut(s) 43
Bme1390I CCNGG 4 cut(s) 138, 384, 597, 603
Bme18I GGWCC 2 cut(s) 491, 659
BmgT120I GGNCC 3 cut(s) 491, 599, 659
BmiI GGNNCC 5 cut(s) 135, 142, 568, 832, 954
BmrFI CCNGG 4 cut(s) 138, 384, 597, 603
BmrI ACTGGG 2 cut(s) 462, 897
BmsI GCATC 3 cut(s) 193, 628, 677
BmuI ACTGGG 2 cut(s) 462, 897
Bpu10I CCTNAGC 1 cut(s) 643
BpuEI CTTGAG 2 cut(s) 51, 170
BpuMI CCSGG 2 cut(s) 138, 597
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 150, 373, 601, 692
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 602, 648, 907
Bse118I RCCGGY 3 cut(s) 167, 457, 542
Bse1I ACTGG 5 cut(s) 39, 468, 626, 721, 892
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 384, 603
BseDI CCNNGG 4 cut(s) 150, 373, 601, 692
BseGI GGATG 2 cut(s) 88, 1032
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 4 cut(s) 137, 602, 648, 907
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 2 cut(s) 657, 917
BseNI ACTGG 5 cut(s) 39, 468, 626, 721, 892
BseXI GCAGC 1 cut(s) 697
BseYI CCCAGC 2 cut(s) 647, 1036
BshFI GGCC 4 cut(s) 171, 457, 600, 720
BshNI GGYRCC 1 cut(s) 566
BsiSI CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
BslFI GGGAC 2 cut(s) 450, 904
BslI CCNNNNNNNGG 4 cut(s) 137, 602, 648, 907
BsmFI GGGAC 2 cut(s) 450, 904
BsnI GGCC 4 cut(s) 171, 457, 600, 720
Bsp1286I GDGCHC 1 cut(s) 835
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 5 cut(s) 6, 140, 307, 423, 952
Bsp19I CCATGG 2 cut(s) 150, 692
BspACI CCGC 4 cut(s) 278, 531, 730, 1078
BspANI GGCC 4 cut(s) 171, 457, 600, 720
BspCNI CTCAG 2 cut(s) 656, 916
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 5 cut(s) 135, 142, 568, 832, 954
BspPI GGATC 5 cut(s) 14, 135, 148, 947, 960
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 1067
BsrBI CCGCTC 1 cut(s) 1078
BsrDI GCAATG 1 cut(s) 763
BsrFI RCCGGY 3 cut(s) 167, 457, 542
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 5 cut(s) 39, 468, 626, 721, 892
BssAI RCCGGY 3 cut(s) 167, 457, 542
BssECI CCNNGG 4 cut(s) 150, 373, 601, 692
BssMI GATC 5 cut(s) 6, 140, 307, 423, 952
BssT1I CCWWGG 3 cut(s) 150, 373, 692
Bst2UI CCWGG 2 cut(s) 384, 603
Bst4CI ACNGT 2 cut(s) 706, 1019
BstAFI CTTAAG 1 cut(s) 1067
BstAUI TGTACA 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 169, 459
BstDEI CTNAG 3 cut(s) 211, 643, 903
BstDSI CCRYGG 2 cut(s) 150, 692
BstF5I GGATG 2 cut(s) 88, 1032
BstKTI GATC 5 cut(s) 9, 143, 310, 426, 955
BstMBI GATC 5 cut(s) 6, 140, 307, 423, 952
BstMWI GCNNNNNNNGC 3 cut(s) 62, 212, 255
BstNI CCWGG 2 cut(s) 384, 603
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 4 cut(s) 136, 382, 595, 601
BstV1I GCAGC 1 cut(s) 697
BstX2I RGATCY 3 cut(s) 6, 140, 952
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 3 cut(s) 6, 140, 952
BsuI GTATCC 3 cut(s) 46, 232, 825
BsuRI GGCC 4 cut(s) 171, 457, 600, 720
BtgI CCRYGG 2 cut(s) 150, 692
BtsCI GGATG 2 cut(s) 88, 1032
BtsIMutI CAGTG 3 cut(s) 327, 633, 885
Cac8I GCNNGC 2 cut(s) 169, 459
CciI TCATGA 1 cut(s) 156
Cfr10I RCCGGY 3 cut(s) 167, 457, 542
Cfr13I GGNCC 3 cut(s) 491, 599, 659
Csp6I GTAC 2 cut(s) 42, 115
CviAII CATG 9 cut(s) 118, 151, 157, 173, 281, 363, 580, 592, 693
CviQI GTAC 2 cut(s) 42, 115
DdeI CTNAG 3 cut(s) 211, 643, 903
DpnI GATC 5 cut(s) 8, 142, 309, 425, 954
DpnII GATC 5 cut(s) 6, 140, 307, 423, 952
DraI TTTAAA 1 cut(s) 897
EaeI YGGCCR 2 cut(s) 169, 455
Eco130I CCWWGG 3 cut(s) 150, 373, 692
Eco24I GRGCYC 1 cut(s) 835
Eco32I GATATC 1 cut(s) 17
Eco47I GGWCC 2 cut(s) 491, 659
Eco57I CTGAAG 1 cut(s) 837
EcoRII CCWGG 2 cut(s) 382, 601
EcoRV GATATC 1 cut(s) 17
EcoT14I CCWWGG 3 cut(s) 150, 373, 692
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 835
ErhI CCWWGG 3 cut(s) 150, 373, 692
FaeI CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 696
FaqI GGGAC 2 cut(s) 450, 904
FatI CATG 9 cut(s) 117, 150, 156, 172, 280, 362, 579, 591, 692
Fnu4HI GCNGC 2 cut(s) 278, 711
FokI GGATG 2 cut(s) 95, 1019
FriOI GRGCYC 1 cut(s) 835
Fsp4HI GCNGC 2 cut(s) 278, 711
FspBI CTAG 5 cut(s) 20, 26, 374, 621, 743
GluI GCNGC 2 cut(s) 278, 711
GsaI CCCAGC 2 cut(s) 651, 1040
HaeIII GGCC 4 cut(s) 171, 457, 600, 720
HapII CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
Hin1II CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 696
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 2 cut(s) 479, 739
HpaII CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
HphI GGTGA 3 cut(s) 532, 787, 922
Hpy166II GTNNAC 3 cut(s) 115, 586, 631
Hpy188I TCNGA 5 cut(s) 301, 491, 681, 856, 906
Hpy188III TCNNGA 4 cut(s) 157, 187, 774, 950
Hpy8I GTNNAC 3 cut(s) 115, 586, 631
HpyAV CCTTC 6 cut(s) 237, 520, 802, 901, 907, 912
HpyCH4III ACNGT 2 cut(s) 706, 1019
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 4 cut(s) 668, 689, 872, 933
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 212, 255
HpyF3I CTNAG 3 cut(s) 211, 643, 903
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 696
KroI GCCGGC 2 cut(s) 167, 457
KroNI GCCGGC 2 cut(s) 169, 459
Kzo9I GATC 5 cut(s) 6, 140, 307, 423, 952
LmnI GCTCC 2 cut(s) 246, 830
Lsp1109I GCAGC 1 cut(s) 697
LweI GCATC 3 cut(s) 193, 628, 677
MaeI CTAG 5 cut(s) 20, 26, 374, 621, 743
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 6 cut(s) 533, 548, 605, 847, 881, 1019
MalI GATC 5 cut(s) 8, 142, 309, 425, 954
MbiI CCGCTC 1 cut(s) 1078
MboI GATC 5 cut(s) 6, 140, 307, 423, 952
MboII GAAGA 3 cut(s) 406, 663, 1010
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 3 cut(s) 6, 140, 952
MhlI GDGCHC 1 cut(s) 835
MluCI AATT 7 cut(s) 195, 228, 344, 450, 561, 768, 778
MlyI GAGTC 1 cut(s) 733
MmeI TCCRAC 2 cut(s) 867, 980
MnlI CCTC 6 cut(s) 73, 154, 604, 652, 869, 912
Mph1103I ATGCAT 1 cut(s) 670
MroNI GCCGGC 2 cut(s) 167, 457
MseI TTAA 7 cut(s) 734, 747, 896, 963, 971, 1056, 1068
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 647
MspCI CTTAAG 1 cut(s) 1067
MspI CCGG 6 cut(s) 131, 138, 168, 458, 543, 597
MspR9I CCNGG 4 cut(s) 138, 384, 597, 603
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 2 cut(s) 384, 603
MwoI GCNNNNNNNGC 3 cut(s) 62, 212, 255
NaeI GCCGGC 2 cut(s) 169, 459
NciI CCSGG 2 cut(s) 138, 597
NcoI CCATGG 2 cut(s) 150, 692
NdeII GATC 5 cut(s) 6, 140, 307, 423, 952
NgoMIV GCCGGC 2 cut(s) 167, 457
NlaIII CATG 9 cut(s) 121, 154, 160, 176, 284, 366, 583, 595, 696
NlaIV GGNNCC 5 cut(s) 135, 142, 568, 832, 954
NmuCI GTSAC 1 cut(s) 881
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 595
PagI TCATGA 1 cut(s) 156
PdiI GCCGGC 2 cut(s) 169, 459
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 279, 712
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
Psp1406I AACGTT 1 cut(s) 846
Psp6I CCWGG 2 cut(s) 382, 601
PspFI CCCAGC 2 cut(s) 647, 1036
PspGI CCWGG 2 cut(s) 382, 601
PspN4I GGNNCC 5 cut(s) 135, 142, 568, 832, 954
PspPI GGNCC 3 cut(s) 491, 599, 659
PsuI RGATCY 3 cut(s) 6, 140, 952
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 43, 116
RsaNI GTAC 2 cut(s) 42, 115
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 7 cut(s) 734, 747, 896, 963, 971, 1056, 1068
SatI GCNGC 2 cut(s) 278, 711
Sau3AI GATC 5 cut(s) 6, 140, 307, 423, 952
Sau96I GGNCC 3 cut(s) 491, 599, 659
ScaI AGTACT 1 cut(s) 43
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 4 cut(s) 138, 384, 597, 603
SduI GDGCHC 1 cut(s) 835
SfaNI GCATC 3 cut(s) 193, 628, 677
SinI GGWCC 2 cut(s) 491, 659
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 3 cut(s) 66, 185, 1067
SmoI CTYRAG 3 cut(s) 66, 185, 1067
Sse9I AATT 7 cut(s) 195, 228, 344, 450, 561, 768, 778
SsiI CCGC 4 cut(s) 278, 531, 730, 1078
SspMI CTAG 5 cut(s) 20, 26, 374, 621, 743
StyD4I CCNGG 4 cut(s) 136, 382, 595, 601
StyI CCWWGG 3 cut(s) 150, 373, 692
TaaI ACNGT 2 cut(s) 706, 1019
TaiI ACGT 1 cut(s) 849
TasI AATT 7 cut(s) 195, 228, 344, 450, 561, 768, 778
TatI WGTACW 2 cut(s) 41, 114
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 7 cut(s) 734, 747, 896, 963, 971, 1056, 1068
Tru9I TTAA 7 cut(s) 734, 747, 896, 963, 971, 1056, 1068
TscAI CASTG 3 cut(s) 327, 640, 892
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 1 cut(s) 710
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 5 cut(s) 297, 488, 539, 568, 1017
TspGWI ACGGA 1 cut(s) 879
TspRI CASTG 3 cut(s) 327, 640, 892
Vha464I CTTAAG 1 cut(s) 1067
VpaK11BI GGWCC 2 cut(s) 491, 659
XapI RAATTY 4 cut(s) 228, 450, 768, 778
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmaJI CCTAGG 1 cut(s) 373
XspI CTAG 5 cut(s) 20, 26, 374, 621, 743
ZrmI AGTACT 1 cut(s) 43
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.