Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0006498

Physical Location & Seq

Genomic Coordinates Forward (+)

17187816 .. 17191651

Gene Structure 3836 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G016290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGTGGATCTTACAATATCTAGTGCTAGTATTGGTATCCAGTACTTGTGTTGTAGCTACATTGGCTCAAGGAACAAACGAGGATGGTTCTGGCTTTAGGAAGCCTTACATTGTATACATGGGAGAACTGCCGGAACCCGGATCCTCCACCATGGTCATGACCACAGCCGGCCATGATAACTTGCTCAAGACAGCAATTGGAGATGCCGACTTAGCCAGAGTATCCAAAATTTATACTTATGGAAGGAGCTTCAACGGCTTCGTAGCCAGATTGTTGCCGCATGAAGCAAAGAAACTATCAGGTGCTGATAATTTTAAACTCTCTCTGTCCTTTGTAAATGATGATCGGGTTGTTTCAGTGTTTCCAAACACCAAGCTCAAATTACATACAACAAGGTCATGGGATTTCCTAGGATTGCCAGGAAATACGAAGAAACGAAATCTTGCCAATAGAGAGCAAGATCGTAGAATTTGGCCGGCTTGTCCGAGTATGAACGATTCAGGCTTCGGACCACCCCCAAGAAAATGGAAAGGAAAATGTATGAAGGGCGGTAACTTCACCGGCTGTAACAACAAGGTAATTGGTGCCAAATACTTCATGGTGGACGACATGCCTGGCCCTGGTTACGAGGACAACGCTAGTCCAGTTGACACTGATGGCCATGGCACTCACACAGCGTCCATTGTTGGTGGTGTACCCGTCCGAGGCGCCAGCCTATATGGCATTGCAAAAGGGACTGCTCGTGGGGGAGCGCCATCGTCACGTATAGCCACGTACAAAGCATGCTGGACTGGTGGCTGCGGTGACATGAACATCTTGGCCGCGTTTGATGAAGCCATTGCTGATGGTGTGGACTTGATATCAATCTCCGTTGGCGGTCCTTCACGGAATTTCCTCCAGGATTCCATTGCTCTCGGATCGTTTCATGCCATGAAGAGAGGGATTTTGGTTTCAGCCTCAGCTGGGAATGACGGACCTTATGCATCCTCTGTTCAGAATGTTGCACCATGGATAATGACTGTTGCTGCTACTGGCCTTGATAGGCGGTTAAAGACTCTAGTTAAGTTGGGTAATGGCAAGAAAATTTCTGGAAATTCAATCAATACCTTCACCCCCAAGAAACAGATGTATCCCCTCACAAGTGGAGCCCGTGCTTCCAACGTTACTTCAGACCTCTACGGAAATGCGAGCCCTCAGAAGGTGAAGGGAAGGATTGTGTTGTGCAAAGGGCAAAGTAATCAGGATCCAACTATTCAAGACTTAAATGGGGCAGGAACTATAATGACAGAAGATGAAATGCTTGATTACGGTTACACCCCTATCATCCCAGCTACCTATGTGCTTGTTAATGATGGACTTAAGATTGAGCGGTATATAAACACTACCAAGAACCCTACGGCTGTAATACAGCGGACAAGAACTGTTCATATGGATGCCCCCTTCATTGCTTCTTTCTCTTCCAGAGGACCTCAACTCATAACCCCCAACATCCTCAAGCCTGATCTTGCTGCACCAGGGATAAACATACTAGCTGCATATTCGAAGCTGCCATCTTTAACAGGTAGCCTAGAAGACATGCGCCACTATTCGTTCAACATACTCTCAGGTACATCCATGTCTTGCCCTCATGCAACTGCCTCAGCAGCCTATGTCAAGTCCTTCCACCCGGACTGGTCCCCGGCTGCCATCAAGTCGGCTCTCATGACAACCGCCACACCATTGAAAATCAAACCCCTGGAAGGCGAGTTAGCCTTCGGAGCAGGACAAATACAGCCAATAAAGGCAGTGCATCCTGGCCTCGTCTACGACATTTCCATGAACGACTACATCAGCTTCTTGTGCAAGGAAGGCTACAACAGCACCAGCATTGGAAAGCTTATTGGTGGTAAAAAGAAGTACAACTGCTCAGACTTTAAACCCGCACCCGGCACCGATGGCCTCAACTATCCTTCAATGTCCTACCTGGTGCGAAATCCGAATTCTAGCATTTCAGCTGTTTTCCTTAGATCGGTGAAGCATGTAGGGTACGGTAAGTCGGTGTACAAGGCAAAGGTGACTTCGCCAAAGGGCCTTTCCATTAGAGTCACCCCGAGCACCTTGACATTCACTAAGAAGCATCAGAAGCGATCCTTCACGGTTGTGGTGAAGGGTGGCACGATGAAAGGAAAGATAGAGAGTCTGTCTGCTCTACTAGAGTGGGATGATGGTAGGCATACGGTTAGGAGCCCCATCGTAGTCTACAGGCATACCACATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

752

Amino Acids

80.86

Weight (kDa)

9.59

Isoelectric Point (pI)

31.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	36 - 129	1.5e-08	Peptidase inhibitor I9
Peptidase_S8	PF00082	199 - 586	1.9e-42	Subtilase family
fn3_6	PF17766	647 - 746	3.6e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006498)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03620 AT5G03620
malus_domestica	MD12G1022400.v1.1 MD12G1022500.v1.1 MD14G1019300.v1.1 MD14G1019400.v1.1
prunus_persica	Prupe.7G114300_v2.0.a1 Prupe.7G114300_v2.0.a1
pyrus_communis	pycom14g01900
rosa_chinensis	RchiOBHm_Chr3g0472671 RchiOBHm_Chr3g0472681
rosa_laevigata	RLG00000016130 RLG00000024064
rosa_multiflora	Rmu_sc0001616.1_g000054 Rmu_sc0003510.1_g000013
rosa_roxburghii	Rroxscaffold_5G00353850 Rroxscaffold_6G00408720 Rroxscaffold_6G00408730
rosa_rugosa	Rorug03G0127100 Rorug04G0092600
rosa_samantha	Rh3AG179800 Rh3BG205700 Rh3CG202800 Rh3DG170000 Rh3DG170100
rosa_wichuraiana	Rw3G016290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	584, 707, 1928
AccBSI	CCGCTC	1 cut(s)	1369
AccI	GTMKAC	3 cut(s)	114, 1803, 2238
AccII	CGCG	1 cut(s)	824
AclI	AACGTT	1 cut(s)	1161
AclWI	GGATC	7 cut(s)	14, 135, 148, 925, 1238, 1251, 2121
AcoI	YGGCCR	4 cut(s)	169, 473, 658, 819
AcsI	RAATTY	6 cut(s)	228, 468, 889, 1083, 1093, 1978
AcuI	CTGAAG	1 cut(s)	1152
AcyI	GRCGYC	1 cut(s)	708
AfaI	GTAC	7 cut(s)	43, 696, 776, 1609, 1898, 2027, 2042
AfiI	CCNNNNNNNGG	8 cut(s)	137, 620, 704, 963, 1198, 1739, 1925, 2008
AflII	CTTAAG	1 cut(s)	1358
AgsI	TTSAA	6 cut(s)	253, 1098, 1256, 1594, 1723, 1953
AjnI	CCWGG	8 cut(s)	418, 613, 619, 897, 1513, 1734, 1792, 1962
AjuI	GAANNNNNNNTTGG	2 cut(s)	2056, 2088
AleI	CACNNNNGTG	1 cut(s)	2138
Alw21I	GWGCWC	1 cut(s)	2096
AlwI	GGATC	7 cut(s)	14, 135, 148, 925, 1238, 1251, 2121
AlwNI	CAGNNNCTG	1 cut(s)	305
Ama87I	CYCGRG	1 cut(s)	2089
AoxI	GGCC	9 cut(s)	169, 473, 616, 658, 819, 1033, 1795, 1936, 2068
ApeKI	GCWGC	7 cut(s)	798, 1025, 1508, 1532, 1546, 1643, 1682
ApoI	RAATTY	6 cut(s)	228, 468, 889, 1083, 1093, 1978
AspA2I	CCTAGG	1 cut(s)	409
AspLEI	GCGC	3 cut(s)	710, 754, 1581
AspS9I	GGNCC	7 cut(s)	509, 617, 878, 974, 1466, 1674, 2068
AsuC2I	CCSGG	4 cut(s)	138, 1667, 1679, 1926
AsuHPI	GGTGA	8 cut(s)	550, 815, 1102, 1213, 2023, 2065, 2077, 2155
AsuII	TTCGAA	1 cut(s)	1541
AvaI	CYCGRG	1 cut(s)	2089
AvaII	GGWCC	5 cut(s)	509, 878, 974, 1466, 1674
AvrII	CCTAGG	1 cut(s)	409
BaeI	ACNNNNGTAYC	2 cut(s)	1112, 1145
BalI	TGGCCA	1 cut(s)	660
BamHI	GGATCC	2 cut(s)	140, 1243
BanI	GGYRCC	3 cut(s)	584, 707, 1928
BanII	GRGCYC	3 cut(s)	1150, 1193, 2228
BauI	CACGAG	1 cut(s)	741
BbsI	GAAGAC	1 cut(s)	1578
Bbv12I	GWGCWC	1 cut(s)	2096
BbvCI	CCTCAGC	2 cut(s)	958, 1639
BbvI	GCAGC	7 cut(s)	785, 1012, 1495, 1519, 1533, 1655, 1669
BceAI	ACGGC	2 cut(s)	271, 1413
BciT130I	CCWGG	8 cut(s)	420, 615, 621, 899, 1515, 1736, 1794, 1964
BciVI	GTATCC	3 cut(s)	46, 232, 1140
BcnI	CCSGG	4 cut(s)	138, 1667, 1679, 1926
BfaI	CTAG	9 cut(s)	20, 26, 410, 639, 1058, 1529, 1568, 1983, 2192
BfmI	CTRYAG	1 cut(s)	2239
BfoI	RGCGCY	2 cut(s)	711, 755
BfrI	CTTAAG	1 cut(s)	1358
BfuI	GTATCC	3 cut(s)	46, 232, 1140
BglI	GCCNNNNNGGC	1 cut(s)	720
BisI	GCNGC	9 cut(s)	278, 799, 822, 1026, 1509, 1533, 1547, 1644, 1683
BlnI	CCTAGG	1 cut(s)	409
BlsI	GCNGC	9 cut(s)	279, 800, 823, 1027, 1510, 1534, 1548, 1645, 1684
BmcAI	AGTACT	1 cut(s)	43
Bme18I	GGWCC	5 cut(s)	509, 878, 974, 1466, 1674
BmeT110I	CYCGRG	1 cut(s)	2089
BmgT120I	GGNCC	7 cut(s)	509, 617, 878, 974, 1466, 1674, 2068
BmiI	GGNNCC	9 cut(s)	135, 142, 586, 709, 1147, 1245, 1676, 1930, 2225
BmsI	GCATC	5 cut(s)	193, 992, 1423, 1798, 2125
BpiI	GAAGAC	1 cut(s)	1578
BpmI	CTGGAG	1 cut(s)	881
Bpu10I	CCTNAGC	2 cut(s)	958, 1639
Bpu14I	TTCGAA	1 cut(s)	1541
BpuEI	CTTGAG	3 cut(s)	51, 170, 1478
BpuMI	CCSGG	4 cut(s)	138, 1667, 1679, 1926
BsaAI	YACGTR	2 cut(s)	764, 774
BsaBI	GATNNNNATC	1 cut(s)	863
BsaHI	GRCGYC	1 cut(s)	708
BsaJI	CCNNGG	9 cut(s)	150, 409, 619, 661, 703, 1007, 1514, 1677, 1734
Bsc4I	CCNNNNNNNGG	8 cut(s)	137, 620, 704, 963, 1198, 1739, 1925, 2008
Bse118I	RCCGGY	3 cut(s)	167, 475, 560
Bse1I	ACTGG	5 cut(s)	39, 644, 796, 1036, 1676
Bse3DI	GCAATG	4 cut(s)	723, 837, 906, 1443
Bse8I	GATNNNNATC	1 cut(s)	863
BseBI	CCWGG	8 cut(s)	420, 615, 621, 899, 1515, 1736, 1794, 1964
BseDI	CCNNGG	9 cut(s)	150, 409, 619, 661, 703, 1007, 1514, 1677, 1734
BseGI	GGATG	8 cut(s)	88, 983, 1323, 1438, 1488, 1610, 1789, 2206
BseJI	GATNNNNATC	1 cut(s)	863
BseLI	CCNNNNNNNGG	8 cut(s)	137, 620, 704, 963, 1198, 1739, 1925, 2008
BseMI	GCAATG	4 cut(s)	723, 837, 906, 1443
BseMII	CTCAG	5 cut(s)	972, 1208, 1617, 1653, 1920
BseNI	ACTGG	5 cut(s)	39, 644, 796, 1036, 1676
BseXI	GCAGC	7 cut(s)	785, 1012, 1495, 1519, 1533, 1655, 1669
BseYI	CCCAGC	2 cut(s)	962, 1327
BsgI	GTGCAG	1 cut(s)	1494
Bsh1236I	CGCG	1 cut(s)	824
BshFI	GGCC	9 cut(s)	171, 475, 618, 660, 821, 1035, 1797, 1938, 2070
BshNI	GGYRCC	3 cut(s)	584, 707, 1928
BsiHKAI	GWGCWC	1 cut(s)	2096
BsiHKCI	CYCGRG	1 cut(s)	2089
BsiSI	CCGG	8 cut(s)	131, 138, 168, 476, 561, 1667, 1679, 1926
BslFI	GGGAC	2 cut(s)	748, 1660
BslI	CCNNNNNNNGG	8 cut(s)	137, 620, 704, 963, 1198, 1739, 1925, 2008
BsmFI	GGGAC	2 cut(s)	748, 1660
BsnI	GGCC	9 cut(s)	171, 475, 618, 660, 821, 1035, 1797, 1938, 2070
BsoBI	CYCGRG	1 cut(s)	2089
Bsp119I	TTCGAA	1 cut(s)	1541
Bsp1286I	GDGCHC	4 cut(s)	1150, 1193, 2096, 2228
Bsp1407I	TGTACA	1 cut(s)	2040
Bsp143I	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
Bsp19I	CCATGG	3 cut(s)	150, 661, 1007
BspANI	GGCC	9 cut(s)	171, 475, 618, 660, 821, 1035, 1797, 1938, 2070
BspCNI	CTCAG	5 cut(s)	971, 1207, 1616, 1652, 1919
BspFNI	CGCG	1 cut(s)	824
BspHI	TCATGA	2 cut(s)	156, 1701
BspLI	GGNNCC	9 cut(s)	135, 142, 586, 709, 1147, 1245, 1676, 1930, 2225
BspPI	GGATC	7 cut(s)	14, 135, 148, 925, 1238, 1251, 2121
BspT104I	TTCGAA	1 cut(s)	1541
BspT107I	GGYRCC	3 cut(s)	584, 707, 1928
BspTI	CTTAAG	1 cut(s)	1358
BsrBI	CCGCTC	1 cut(s)	1369
BsrDI	GCAATG	4 cut(s)	723, 837, 906, 1443
BsrFI	RCCGGY	3 cut(s)	167, 475, 560
BsrGI	TGTACA	1 cut(s)	2040
BsrI	ACTGG	5 cut(s)	39, 644, 796, 1036, 1676
BssAI	RCCGGY	3 cut(s)	167, 475, 560
BssECI	CCNNGG	9 cut(s)	150, 409, 619, 661, 703, 1007, 1514, 1677, 1734
BssMI	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
BssNAI	GTATAC	1 cut(s)	115
BssNI	GRCGYC	1 cut(s)	708
BssSI	CACGAG	1 cut(s)	741
BssT1I	CCWWGG	4 cut(s)	150, 409, 661, 1007
Bst1107I	GTATAC	1 cut(s)	115
Bst2BI	CACGAG	1 cut(s)	741
Bst2UI	CCWGG	8 cut(s)	420, 615, 621, 899, 1515, 1736, 1794, 1964
Bst4CI	ACNGT	6 cut(s)	1021, 1310, 1423, 2030, 2137, 2218
Bst6I	CTCTTC	2 cut(s)	929, 1462
BstACI	GRCGYC	1 cut(s)	708
BstAFI	CTTAAG	1 cut(s)	1358
BstAUI	TGTACA	1 cut(s)	2040
BstBAI	YACGTR	2 cut(s)	764, 774
BstBI	TTCGAA	1 cut(s)	1541
BstC8I	GCNNGC	5 cut(s)	169, 477, 712, 784, 1189
BstDEI	CTNAG	8 cut(s)	211, 958, 1194, 1603, 1639, 1906, 2003, 2109
BstDSI	CCRYGG	3 cut(s)	150, 661, 1007
BstF5I	GGATG	8 cut(s)	88, 983, 1323, 1438, 1488, 1610, 1789, 2206
BstFNI	CGCG	1 cut(s)	824
BstH2I	RGCGCY	2 cut(s)	711, 755
BstHHI	GCGC	3 cut(s)	710, 754, 1581
BstKTI	GATC	9 cut(s)	9, 143, 346, 463, 920, 1246, 1504, 2009, 2129
BstMBI	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
BstNI	CCWGG	8 cut(s)	420, 615, 621, 899, 1515, 1736, 1794, 1964
BstNSI	RCATGY	4 cut(s)	613, 786, 1579, 2021
BstSFI	CTRYAG	1 cut(s)	2239
BstUI	CGCG	1 cut(s)	824
BstV1I	GCAGC	7 cut(s)	785, 1012, 1495, 1519, 1533, 1655, 1669
BstV2I	GAAGAC	1 cut(s)	1578
BstX2I	RGATCY	3 cut(s)	6, 140, 1243
BstXI	CCANNNNNNTGG	1 cut(s)	525
BstYI	RGATCY	3 cut(s)	6, 140, 1243
BstZ17I	GTATAC	1 cut(s)	115
BsuI	GTATCC	3 cut(s)	46, 232, 1140
BsuRI	GGCC	9 cut(s)	171, 475, 618, 660, 821, 1035, 1797, 1938, 2070
BtgI	CCRYGG	3 cut(s)	150, 661, 1007
BtsCI	GGATG	8 cut(s)	88, 983, 1323, 1438, 1488, 1610, 1789, 2206
BtsI	GCAGTG	1 cut(s)	1791
BtsIMutI	CAGTG	3 cut(s)	363, 651, 1791
Cac8I	GCNNGC	5 cut(s)	169, 477, 712, 784, 1189
CaiI	CAGNNNCTG	1 cut(s)	305
CciI	TCATGA	2 cut(s)	156, 1701
CfoI	GCGC	3 cut(s)	710, 754, 1581
Cfr10I	RCCGGY	3 cut(s)	167, 475, 560
Cfr13I	GGNCC	7 cut(s)	509, 617, 878, 974, 1466, 1674, 2068
CseI	GACGC	1 cut(s)	666
CsiI	ACCWGGT	1 cut(s)	1962
Csp6I	GTAC	7 cut(s)	42, 695, 775, 1608, 1897, 2026, 2041
CviQI	GTAC	7 cut(s)	42, 695, 775, 1608, 1897, 2026, 2041
DdeI	CTNAG	8 cut(s)	211, 958, 1194, 1603, 1639, 1906, 2003, 2109
DinI	GGCGCC	1 cut(s)	709
DpnI	GATC	9 cut(s)	8, 142, 345, 462, 919, 1245, 1503, 2008, 2128
DpnII	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
DraI	TTTAAA	2 cut(s)	316, 1915
EaeI	YGGCCR	4 cut(s)	169, 473, 658, 819
Eam1104I	CTCTTC	2 cut(s)	929, 1462
EarI	CTCTTC	2 cut(s)	929, 1462
Eco130I	CCWWGG	4 cut(s)	150, 409, 661, 1007
Eco24I	GRGCYC	3 cut(s)	1150, 1193, 2228
Eco32I	GATATC	1 cut(s)	861
Eco47I	GGWCC	5 cut(s)	509, 878, 974, 1466, 1674
Eco57I	CTGAAG	1 cut(s)	1152
Eco88I	CYCGRG	1 cut(s)	2089
EcoO109I	RGGNCCY	2 cut(s)	1466, 2068
EcoRI	GAATTC	1 cut(s)	1978
EcoRII	CCWGG	8 cut(s)	418, 613, 619, 897, 1513, 1734, 1792, 1962
EcoRV	GATATC	1 cut(s)	861
EcoT14I	CCWWGG	4 cut(s)	150, 409, 661, 1007
EcoT22I	ATGCAT	1 cut(s)	985
EcoT38I	GRGCYC	3 cut(s)	1150, 1193, 2228
EgeI	GGCGCC	1 cut(s)	709
EheI	GGCGCC	1 cut(s)	709
ErhI	CCWWGG	4 cut(s)	150, 409, 661, 1007
FalI	AAGNNNNNCTT	2 cut(s)	2114, 2146
FaqI	GGGAC	2 cut(s)	748, 1660
FauI	CCCGC	1 cut(s)	1927
FauNDI	CATATG	1 cut(s)	1428
FblI	GTMKAC	3 cut(s)	114, 1803, 2238
Fnu4HI	GCNGC	9 cut(s)	278, 799, 822, 1026, 1509, 1533, 1547, 1644, 1683
FokI	GGATG	8 cut(s)	95, 970, 1310, 1445, 1475, 1597, 1776, 2213
FriOI	GRGCYC	3 cut(s)	1150, 1193, 2228
Fsp4HI	GCNGC	9 cut(s)	278, 799, 822, 1026, 1509, 1533, 1547, 1644, 1683
FspBI	CTAG	9 cut(s)	20, 26, 410, 639, 1058, 1529, 1568, 1983, 2192
GlaI	GCGC	3 cut(s)	709, 753, 1580
GluI	GCNGC	9 cut(s)	278, 799, 822, 1026, 1509, 1533, 1547, 1644, 1683
GsaI	CCCAGC	2 cut(s)	966, 1331
GsuI	CTGGAG	1 cut(s)	881
HaeII	RGCGCY	2 cut(s)	711, 755
HaeIII	GGCC	9 cut(s)	171, 475, 618, 660, 821, 1035, 1797, 1938, 2070
HapII	CCGG	8 cut(s)	131, 138, 168, 476, 561, 1667, 1679, 1926
HgaI	GACGC	1 cut(s)	666
HhaI	GCGC	3 cut(s)	710, 754, 1581
Hin1I	GRCGYC	1 cut(s)	708
Hin6I	GCGC	3 cut(s)	708, 752, 1579
HinP1I	GCGC	3 cut(s)	708, 752, 1579
HincII	GTYRAC	1 cut(s)	649
HindII	GTYRAC	1 cut(s)	649
HindIII	AAGCTT	1 cut(s)	1874
HinfI	GANTC	5 cut(s)	497, 902, 1054, 2082, 2176
HpaII	CCGG	8 cut(s)	131, 138, 168, 476, 561, 1667, 1679, 1926
HphI	GGTGA	8 cut(s)	550, 815, 1102, 1213, 2023, 2065, 2077, 2155
Hpy166II	GTNNAC	8 cut(s)	115, 604, 649, 695, 853, 1804, 2041, 2239
Hpy188III	TCNNGA	7 cut(s)	157, 187, 1089, 1241, 1256, 1461, 1702
Hpy8I	GTNNAC	8 cut(s)	115, 604, 649, 695, 853, 1804, 2041, 2239
HpyCH4III	ACNGT	6 cut(s)	1021, 1310, 1423, 2030, 2137, 2218
HpyCH4IV	ACGT	3 cut(s)	763, 773, 1161
HpyCH4V	TGCA	9 cut(s)	728, 983, 1004, 1224, 1511, 1535, 1631, 1789, 1842
HpyF3I	CTNAG	8 cut(s)	211, 958, 1194, 1603, 1639, 1906, 2003, 2109
HpySE526I	ACGT	3 cut(s)	763, 773, 1161
Hsp92I	GRCGYC	1 cut(s)	708
HspAI	GCGC	3 cut(s)	708, 752, 1579
KasI	GGCGCC	1 cut(s)	707
KroI	GCCGGC	2 cut(s)	167, 475
KroNI	GCCGGC	2 cut(s)	169, 477
Kzo9I	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
LmnI	GCTCC	5 cut(s)	246, 749, 1145, 1757, 2223
Lsp1109I	GCAGC	7 cut(s)	785, 1012, 1495, 1519, 1533, 1655, 1669
LweI	GCATC	5 cut(s)	193, 992, 1423, 1798, 2125
MabI	ACCWGGT	1 cut(s)	1962
MaeI	CTAG	9 cut(s)	20, 26, 410, 639, 1058, 1529, 1568, 1983, 2192
MaeII	ACGT	3 cut(s)	763, 773, 1161
MaeIII	GTNAC	9 cut(s)	551, 566, 623, 759, 803, 1162, 1310, 2053, 2083
MalI	GATC	9 cut(s)	8, 142, 345, 462, 919, 1245, 1503, 2008, 2128
MbiI	CCGCTC	1 cut(s)	1369
MboI	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
MboII	GAAGA	5 cut(s)	442, 946, 1301, 1449, 1583
MfeI	CAATTG	1 cut(s)	195
MflI	RGATCY	3 cut(s)	6, 140, 1243
MhlI	GDGCHC	4 cut(s)	1150, 1193, 2096, 2228
MlsI	TGGCCA	1 cut(s)	660
MluNI	TGGCCA	1 cut(s)	660
Mly113I	GGCGCC	1 cut(s)	708
MlyI	GAGTC	3 cut(s)	1048, 2091, 2185
MmeI	TCCRAC	2 cut(s)	1182, 1271
Mox20I	TGGCCA	1 cut(s)	660
Mph1103I	ATGCAT	1 cut(s)	985
MroNI	GCCGGC	2 cut(s)	167, 475
MscI	TGGCCA	1 cut(s)	660
MseI	TTAA	8 cut(s)	315, 1049, 1062, 1262, 1347, 1359, 1556, 1914
MslI	CAYNNNNRTG	4 cut(s)	155, 1431, 1814, 2138
Msp20I	TGGCCA	1 cut(s)	660
MspA1I	CMGCKG	3 cut(s)	962, 1411, 1994
MspCI	CTTAAG	1 cut(s)	1358
MspI	CCGG	8 cut(s)	131, 138, 168, 476, 561, 1667, 1679, 1926
MunI	CAATTG	1 cut(s)	195
MvaI	CCWGG	8 cut(s)	420, 615, 621, 899, 1515, 1736, 1794, 1964
MvnI	CGCG	1 cut(s)	824
NaeI	GCCGGC	2 cut(s)	169, 477
NarI	GGCGCC	1 cut(s)	708
NciI	CCSGG	4 cut(s)	138, 1667, 1679, 1926
NcoI	CCATGG	3 cut(s)	150, 661, 1007
NdeI	CATATG	1 cut(s)	1428
NdeII	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
NgoMIV	GCCGGC	2 cut(s)	167, 475
NlaIV	GGNNCC	9 cut(s)	135, 142, 586, 709, 1147, 1245, 1676, 1930, 2225
NmuCI	GTSAC	4 cut(s)	759, 803, 2053, 2083
NsiI	ATGCAT	1 cut(s)	985
NspI	RCATGY	4 cut(s)	613, 786, 1579, 2021
NspV	TTCGAA	1 cut(s)	1541
OliI	CACNNNNGTG	1 cut(s)	2138
PaeI	GCATGC	1 cut(s)	786
PagI	TCATGA	2 cut(s)	156, 1701
PdiI	GCCGGC	2 cut(s)	169, 477
PfeI	GAWTC	2 cut(s)	497, 902
PfoI	TCCNGGA	1 cut(s)	897
PkrI	GCNGC	9 cut(s)	279, 800, 823, 1027, 1510, 1534, 1548, 1645, 1684
PleI	GAGTC	3 cut(s)	1048, 2090, 2184
PluTI	GGCGCC	1 cut(s)	711
PpsI	GAGTC	3 cut(s)	1048, 2090, 2184
Ppu21I	YACGTR	2 cut(s)	764, 774
PpuMI	RGGWCCY	1 cut(s)	1466
Psp1406I	AACGTT	1 cut(s)	1161
Psp5II	RGGWCCY	1 cut(s)	1466
Psp6I	CCWGG	8 cut(s)	418, 613, 619, 897, 1513, 1734, 1792, 1962
PspFI	CCCAGC	2 cut(s)	962, 1327
PspGI	CCWGG	8 cut(s)	418, 613, 619, 897, 1513, 1734, 1792, 1962
PspN4I	GGNNCC	9 cut(s)	135, 142, 586, 709, 1147, 1245, 1676, 1930, 2225
PspPI	GGNCC	7 cut(s)	509, 617, 878, 974, 1466, 1674, 2068
PspPPI	RGGWCCY	1 cut(s)	1466
PstNI	CAGNNNCTG	1 cut(s)	305
PsuI	RGATCY	3 cut(s)	6, 140, 1243
PvuII	CAGCTG	2 cut(s)	962, 1994
RsaI	GTAC	7 cut(s)	43, 696, 776, 1609, 1898, 2027, 2042
RsaNI	GTAC	7 cut(s)	42, 695, 775, 1608, 1897, 2026, 2041
RseI	CAYNNNNRTG	4 cut(s)	155, 1431, 1814, 2138
SaqAI	TTAA	8 cut(s)	315, 1049, 1062, 1262, 1347, 1359, 1556, 1914
SatI	GCNGC	9 cut(s)	278, 799, 822, 1026, 1509, 1533, 1547, 1644, 1683
Sau3AI	GATC	9 cut(s)	6, 140, 343, 460, 917, 1243, 1501, 2006, 2126
Sau96I	GGNCC	7 cut(s)	509, 617, 878, 974, 1466, 1674, 2068
ScaI	AGTACT	1 cut(s)	43
SchI	GAGTC	3 cut(s)	1048, 2091, 2185
SduI	GDGCHC	4 cut(s)	1150, 1193, 2096, 2228
SexAI	ACCWGGT	1 cut(s)	1962
SfaNI	GCATC	5 cut(s)	193, 992, 1423, 1798, 2125
SfcI	CTRYAG	1 cut(s)	2239
SfoI	GGCGCC	1 cut(s)	709
SfuI	TTCGAA	1 cut(s)	1541
SinI	GGWCC	5 cut(s)	509, 878, 974, 1466, 1674
SmiMI	CAYNNNNRTG	4 cut(s)	155, 1431, 1814, 2138
SmlI	CTYRAG	4 cut(s)	66, 185, 1358, 1493
SmoI	CTYRAG	4 cut(s)	66, 185, 1358, 1493
SphI	GCATGC	1 cut(s)	786
SspDI	GGCGCC	1 cut(s)	707
SspMI	CTAG	9 cut(s)	20, 26, 410, 639, 1058, 1529, 1568, 1983, 2192
StyI	CCWWGG	4 cut(s)	150, 409, 661, 1007
TaaI	ACNGT	6 cut(s)	1021, 1310, 1423, 2030, 2137, 2218
TaiI	ACGT	3 cut(s)	766, 776, 1164
TaqI	TCGA	1 cut(s)	1541
TatI	WGTACW	3 cut(s)	41, 1896, 2040
TauI	GCSGC	2 cut(s)	280, 824
TfiI	GAWTC	2 cut(s)	497, 902
Tru1I	TTAA	8 cut(s)	315, 1049, 1062, 1262, 1347, 1359, 1556, 1914
Tru9I	TTAA	8 cut(s)	315, 1049, 1062, 1262, 1347, 1359, 1556, 1914
TscAI	CASTG	3 cut(s)	363, 658, 1791
TseFI	GTSAC	4 cut(s)	759, 803, 2053, 2083
TseI	GCWGC	7 cut(s)	798, 1025, 1508, 1532, 1546, 1643, 1682
Tsp45I	GTSAC	4 cut(s)	759, 803, 2053, 2083
TspGWI	ACGGA	4 cut(s)	859, 901, 987, 1194
TspRI	CASTG	3 cut(s)	363, 658, 1791
Vha464I	CTTAAG	1 cut(s)	1358
VpaK11BI	GGWCC	5 cut(s)	509, 878, 974, 1466, 1674
XapI	RAATTY	6 cut(s)	228, 468, 889, 1083, 1093, 1978
XceI	RCATGY	4 cut(s)	613, 786, 1579, 2021
XcmI	CCANNNNNNNNNTGG	1 cut(s)	595
XmaJI	CCTAGG	1 cut(s)	409
XmiI	GTMKAC	3 cut(s)	114, 1803, 2238
XspI	CTAG	9 cut(s)	20, 26, 410, 639, 1058, 1529, 1568, 1983, 2192
ZrmI	AGTACT	1 cut(s)	43
Zsp2I	ATGCAT	1 cut(s)	985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.