Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006415

Physical Location & Seq

Genomic Coordinates Forward (+)

3174831 .. 3176963

Gene Structure 2133 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1028500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1113 bp

ATGAAAATGGCAGCCAAAAATATTGATGCAACTCTGTACGACCGCGTGAAGCAAGTGAAAGAATTCGACGAGTCAAAGATGGGAGTCAAGGGCCTCTCCGATTCTGGCATCACTTCCATCCCTCAATTTTTTATCCACGACCCTCAAACTTTGTCCGATCTCAAACCGCCGTACAAAAACACCACCACCACCATCCCCACGATCGACCTTTCCCACATCAACTCCGCTGCCCACCGCCCCAAAATTATTGACCAAATGAAATCCGCAGCCAAAAACTTCGGTTTCTTCCAAGTCACCAACCACGGGGTGCCTGTTTCTGTTTTGGGCGAAACGGTCAACGCTGTCAAAGCGTTCCACGAGCAGCCCCATGAGGTCAAATCCAAATACTACAAGCGAGAGGAGGGAAAGGGAGTCATGTATGCTAGCAATAACGACTTGTACCGCACGACGGCTGCCAGCTGGCACGACTCACTACAAGTGTGGATGAGTCCGGAGAGGCCAGAGGTGGAGGAGATACCTGAGGCTTGTAGGGAGGAGGTGGTTGCATGGGATTTGCATGCAACCAAGGTAGCGGAGGACGTGATGGAGTTGCTTTCAGAAGGGTTAGGGTTGGAGGGAAGGAGGTTTAAGGAGTTAACGTTTTCGGATAAGAGGGCGCTTTTGGGGCACTGCTATCCTTACTGTCCCCAGCCTGATCGGACTGTGGGGATTAGGGCACATACTGATCCTGGGATTATAACAGTTTTGTTGCAGAACCATGTGCCTGGGTTGCAAGTTAGGCATGGAAATGAGTGGCTGGATGTGAAGCCTGTGCCTGGAGGTTTAATTATTAACGTTGGAGACTTACTTCAGATAATCTCCAACGGGGAGTACACAAGTGTACAACACAGAGTGTTGGCCAACCCTTGGAAAGAAGCTCGGATCTCCGTCGTGATATTTTTCAACCTATCCAAATGGAGAGAAGATGGTTACTGTGGACCTCTTCCGGAGCTTTTGTCACCTGAGAAACCACCAGTTTATCGAGACTTCACTGCCCAAGAATTCCTTGGAAACTTCTATGGCAAAGGAATAGATACCAAGTCTCTGATTGAGAAAATCACTATAAAAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

371

Amino Acids

41.67

Weight (kDa)

6.37

Isoelectric Point (pI)

38.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	65 - 164	4.9e-19	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	223 - 315	4.8e-29	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006415)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g19280 FvH4_6g19280 FvH4_6g19281
malus_domestica	MD12G1028400.v1.1 MD12G1028500.v1.1 MD14G1028700.v1.1
prunus_persica	Prupe.7G102900_v2.0.a1 Prupe.7G103000_v2.0.a1
pyrus_communis	pycom12g02720 pycom12g02730 pycom14g02670 pycom14g02680
rosa_chinensis	RchiOBHm_Chr3g0474491
rosa_laevigata	RLG00000023917 RLG00000023927 RLG00000023928
rosa_multiflora	Rmu_sc0001353.1_g000045 Rmu_sc0039676.1_g000001
rosa_roxburghii	Rroxscaffold_6G00407120 Rroxscaffold_6G00407300
rosa_rugosa	Rorug03G0140600 Rorug03G0141100
rosa_samantha	Rh3AG192000 Rh3BG221500 Rh3CG216200 Rh3DG216900
rosa_wichuraiana	Rw3G017450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	737
AccB1I	GGYRCC	1 cut(s)	307
AccB7I	CCANNNNNTGG	1 cut(s)	906
AccII	CGCG	1 cut(s)	45
AccIII	TCCGGA	2 cut(s)	490, 985
AciI	CCGC	7 cut(s)	43, 167, 225, 235, 264, 442, 572
AclI	AACGTT	2 cut(s)	638, 834
AclWI	GGATC	2 cut(s)	719, 929
AcoI	YGGCCR	1 cut(s)	897
AcsI	RAATTY	2 cut(s)	62, 1040
AcuI	CTGAAG	1 cut(s)	833
AdeI	CACNNNGTG	2 cut(s)	307, 892
AfaI	GTAC	5 cut(s)	38, 173, 440, 872, 882
AfiI	CCNNNNNNNGG	4 cut(s)	303, 448, 815, 906
AgsI	TTSAA	1 cut(s)	943
AjiI	CACGTC	1 cut(s)	580
AjnI	CCWGG	3 cut(s)	727, 763, 814
AluBI	AGCT	3 cut(s)	459, 917, 991
AluI	AGCT	3 cut(s)	459, 917, 991
Alw26I	GTCTC	3 cut(s)	834, 1017, 1086
AlwI	GGATC	2 cut(s)	719, 929
Aor13HI	TCCGGA	2 cut(s)	490, 985
AoxI	GGCC	3 cut(s)	91, 497, 897
ApeKI	GCWGC	5 cut(s)	11, 227, 266, 361, 452
ApoI	RAATTY	2 cut(s)	62, 1040
Asp700I	GAANNNNTTC	1 cut(s)	62
AspLEI	GCGC	1 cut(s)	658
AspS9I	GGNCC	2 cut(s)	91, 977
AsuHPI	GGTGA	2 cut(s)	286, 990
AsuNHI	GCTAGC	1 cut(s)	422
AvaII	GGWCC	1 cut(s)	977
AxyI	CCTNAGG	1 cut(s)	519
BaeGI	GKGCMC	2 cut(s)	669, 718
BalI	TGGCCA	1 cut(s)	899
BanI	GGYRCC	1 cut(s)	307
BauI	CACGAG	1 cut(s)	356
BbvI	GCAGC	5 cut(s)	23, 214, 278, 373, 439
BccI	CCATC	5 cut(s)	73, 125, 200, 577, 959
BceAI	ACGGC	2 cut(s)	154, 465
BciT130I	CCWGG	3 cut(s)	729, 765, 816
BcoDI	GTCTC	3 cut(s)	834, 1017, 1086
BfaI	CTAG	1 cut(s)	423
BfoI	RGCGCY	1 cut(s)	659
BisI	GCNGC	5 cut(s)	12, 228, 267, 362, 453
BlsI	GCNGC	5 cut(s)	13, 229, 268, 363, 454
Bme1390I	CCNGG	3 cut(s)	729, 765, 816
Bme18I	GGWCC	1 cut(s)	977
BmgBI	CACGTC	1 cut(s)	580
BmgT120I	GGNCC	2 cut(s)	91, 977
BmiI	GGNNCC	1 cut(s)	309
BmrFI	CCNGG	3 cut(s)	729, 765, 816
BmsI	GCATC	2 cut(s)	16, 117
BmtI	GCTAGC	1 cut(s)	426
BpmI	CTGGAG	1 cut(s)	837
BsaJI	CCNNGG	6 cut(s)	301, 564, 728, 764, 905, 1045
BsaWI	WCCGGW	2 cut(s)	490, 985
BsaXI	ACNNNNNCTCC	6 cut(s)	206, 236, 623, 653, 980, 1010
Bsc4I	CCNNNNNNNGG	4 cut(s)	303, 448, 815, 906
Bse1I	ACTGG	1 cut(s)	1013
Bse21I	CCTNAGG	1 cut(s)	519
BseAI	TCCGGA	2 cut(s)	490, 985
BseBI	CCWGG	3 cut(s)	729, 765, 816
BseDI	CCNNGG	6 cut(s)	301, 564, 728, 764, 905, 1045
BseGI	GGATG	4 cut(s)	117, 192, 489, 805
BseLI	CCNNNNNNNGG	4 cut(s)	303, 448, 815, 906
BseMII	CTCAG	2 cut(s)	510, 993
BseNI	ACTGG	1 cut(s)	1013
BseRI	GAGGAG	3 cut(s)	413, 524, 548
BseSI	GKGCMC	2 cut(s)	669, 718
BseXI	GCAGC	5 cut(s)	23, 214, 278, 373, 439
BseYI	CCCAGC	1 cut(s)	687
Bsh1236I	CGCG	1 cut(s)	45
Bsh1285I	CGRYCG	2 cut(s)	43, 204
BshFI	GGCC	3 cut(s)	93, 499, 899
BshNI	GGYRCC	1 cut(s)	307
BsiEI	CGRYCG	2 cut(s)	43, 204
BsiSI	CCGG	2 cut(s)	491, 986
BslFI	GGGAC	1 cut(s)	669
BslI	CCNNNNNNNGG	4 cut(s)	303, 448, 815, 906
BsmAI	GTCTC	3 cut(s)	834, 1017, 1086
BsmFI	GGGAC	1 cut(s)	669
BsnI	GGCC	3 cut(s)	93, 499, 899
Bsp1286I	GDGCHC	2 cut(s)	669, 718
Bsp13I	TCCGGA	2 cut(s)	490, 985
Bsp1407I	TGTACA	1 cut(s)	880
Bsp143I	GATC	5 cut(s)	157, 201, 694, 724, 921
BspACI	CCGC	7 cut(s)	43, 167, 225, 235, 264, 442, 572
BspANI	GGCC	3 cut(s)	93, 499, 899
BspCNI	CTCAG	2 cut(s)	511, 994
BspEI	TCCGGA	2 cut(s)	490, 985
BspFNI	CGCG	1 cut(s)	45
BspLI	GGNNCC	1 cut(s)	309
BspOI	GCTAGC	1 cut(s)	426
BspPI	GGATC	2 cut(s)	719, 929
BspT107I	GGYRCC	1 cut(s)	307
BsrGI	TGTACA	1 cut(s)	880
BsrI	ACTGG	1 cut(s)	1013
BssECI	CCNNGG	6 cut(s)	301, 564, 728, 764, 905, 1045
BssMI	GATC	5 cut(s)	157, 201, 694, 724, 921
BssSI	CACGAG	1 cut(s)	356
BssT1I	CCWWGG	3 cut(s)	564, 905, 1045
Bst2BI	CACGAG	1 cut(s)	356
Bst2UI	CCWGG	3 cut(s)	729, 765, 816
Bst4CI	ACNGT	5 cut(s)	334, 683, 703, 742, 974
Bst6I	CTCTTC	1 cut(s)	987
BstAUI	TGTACA	1 cut(s)	880
BstC8I	GCNNGC	4 cut(s)	424, 457, 461, 558
BstDEI	CTNAG	2 cut(s)	519, 1002
BstDSI	CCRYGG	1 cut(s)	301
BstF5I	GGATG	4 cut(s)	117, 192, 489, 805
BstFNI	CGCG	1 cut(s)	45
BstH2I	RGCGCY	1 cut(s)	659
BstHHI	GCGC	1 cut(s)	658
BstKTI	GATC	5 cut(s)	160, 204, 697, 727, 924
BstMAI	GTCTC	3 cut(s)	834, 1017, 1086
BstMBI	GATC	5 cut(s)	157, 201, 694, 724, 921
BstMCI	CGRYCG	2 cut(s)	43, 204
BstMWI	GCNNNNNNNGC	4 cut(s)	347, 664, 769, 778
BstNI	CCWGG	3 cut(s)	729, 765, 816
BstNSI	RCATGY	1 cut(s)	560
BstSCI	CCNGG	3 cut(s)	727, 763, 814
BstSLI	GKGCMC	2 cut(s)	669, 718
BstUI	CGCG	1 cut(s)	45
BstV1I	GCAGC	5 cut(s)	23, 214, 278, 373, 439
BstX2I	RGATCY	1 cut(s)	921
BstXI	CCANNNNNNTGG	1 cut(s)	764
BstYI	RGATCY	1 cut(s)	921
Bsu36I	CCTNAGG	1 cut(s)	519
BsuRI	GGCC	3 cut(s)	93, 499, 899
BtgI	CCRYGG	1 cut(s)	301
BtrI	CACGTC	1 cut(s)	580
BtsCI	GGATG	4 cut(s)	117, 192, 489, 805
BtsI	GCAGTG	2 cut(s)	667, 1029
BtsIMutI	CAGTG	2 cut(s)	667, 1029
Cac8I	GCNNGC	4 cut(s)	424, 457, 461, 558
CfoI	GCGC	1 cut(s)	658
Cfr13I	GGNCC	2 cut(s)	91, 977
Csp6I	GTAC	5 cut(s)	37, 172, 439, 871, 881
CviAII	CATG	6 cut(s)	368, 415, 546, 557, 758, 782
CviQI	GTAC	5 cut(s)	37, 172, 439, 871, 881
DdeI	CTNAG	2 cut(s)	519, 1002
DpnI	GATC	5 cut(s)	159, 203, 696, 726, 923
DpnII	GATC	5 cut(s)	157, 201, 694, 724, 921
DraIII	CACNNNGTG	2 cut(s)	307, 892
EaeI	YGGCCR	1 cut(s)	897
Eam1104I	CTCTTC	1 cut(s)	987
EarI	CTCTTC	1 cut(s)	987
Eco130I	CCWWGG	3 cut(s)	564, 905, 1045
Eco47I	GGWCC	1 cut(s)	977
Eco57I	CTGAAG	1 cut(s)	833
Eco81I	CCTNAGG	1 cut(s)	519
EcoO109I	RGGNCCY	1 cut(s)	91
EcoRI	GAATTC	2 cut(s)	62, 1040
EcoRII	CCWGG	3 cut(s)	727, 763, 814
EcoT14I	CCWWGG	3 cut(s)	564, 905, 1045
ErhI	CCWWGG	3 cut(s)	564, 905, 1045
FaeI	CATG	6 cut(s)	371, 418, 549, 560, 761, 785
FalI	AAGNNNNNCTT	2 cut(s)	1029, 1061
FaqI	GGGAC	1 cut(s)	669
FatI	CATG	6 cut(s)	367, 414, 545, 556, 757, 781
Fnu4HI	GCNGC	5 cut(s)	12, 228, 267, 362, 453
FokI	GGATG	4 cut(s)	104, 179, 496, 812
Fsp4HI	GCNGC	5 cut(s)	12, 228, 267, 362, 453
FspBI	CTAG	1 cut(s)	423
GlaI	GCGC	1 cut(s)	657
GluI	GCNGC	5 cut(s)	12, 228, 267, 362, 453
GsaI	CCCAGC	1 cut(s)	691
GsuI	CTGGAG	1 cut(s)	837
HaeII	RGCGCY	1 cut(s)	659
HaeIII	GGCC	3 cut(s)	93, 499, 899
HapII	CCGG	2 cut(s)	491, 986
HhaI	GCGC	1 cut(s)	658
Hin1II	CATG	6 cut(s)	371, 418, 549, 560, 761, 785
Hin6I	GCGC	1 cut(s)	656
HinP1I	GCGC	1 cut(s)	656
HincII	GTYRAC	2 cut(s)	337, 636
HindII	GTYRAC	2 cut(s)	337, 636
HinfI	GANTC	6 cut(s)	71, 84, 101, 411, 467, 487
HpaI	GTTAAC	1 cut(s)	636
HpaII	CCGG	2 cut(s)	491, 986
HphI	GGTGA	2 cut(s)	286, 990
Hpy166II	GTNNAC	5 cut(s)	337, 636, 873, 881, 977
Hpy188I	TCNGA	8 cut(s)	100, 157, 598, 646, 699, 852, 921, 1086
Hpy188III	TCNNGA	4 cut(s)	491, 931, 986, 1022
Hpy8I	GTNNAC	5 cut(s)	337, 636, 873, 881, 977
Hpy99I	CGWCG	3 cut(s)	71, 451, 932
HpyAV	CCTTC	2 cut(s)	593, 612
HpyCH4III	ACNGT	5 cut(s)	334, 683, 703, 742, 974
HpyCH4IV	ACGT	3 cut(s)	579, 638, 834
HpyCH4V	TGCA	6 cut(s)	29, 545, 556, 560, 751, 772
HpyF10VI	GCNNNNNNNGC	4 cut(s)	347, 664, 769, 778
HpyF3I	CTNAG	2 cut(s)	519, 1002
HpySE526I	ACGT	3 cut(s)	579, 638, 834
Hsp92II	CATG	6 cut(s)	371, 418, 549, 560, 761, 785
HspAI	GCGC	1 cut(s)	656
Kpn2I	TCCGGA	2 cut(s)	490, 985
KspAI	GTTAAC	1 cut(s)	636
Kzo9I	GATC	5 cut(s)	157, 201, 694, 724, 921
LmnI	GCTCC	1 cut(s)	988
Lsp1109I	GCAGC	5 cut(s)	23, 214, 278, 373, 439
LweI	GCATC	2 cut(s)	16, 117
MaeI	CTAG	1 cut(s)	423
MaeII	ACGT	3 cut(s)	579, 638, 834
MaeIII	GTNAC	3 cut(s)	292, 968, 996
MalI	GATC	5 cut(s)	159, 203, 696, 726, 923
MboI	GATC	5 cut(s)	157, 201, 694, 724, 921
MboII	GAAGA	3 cut(s)	277, 974, 974
MflI	RGATCY	1 cut(s)	921
MhlI	GDGCHC	2 cut(s)	669, 718
MlsI	TGGCCA	1 cut(s)	899
MluCI	AATT	5 cut(s)	62, 125, 243, 825, 1040
MluNI	TGGCCA	1 cut(s)	899
MlyI	GAGTC	5 cut(s)	80, 93, 420, 461, 496
MmeI	TCCRAC	3 cut(s)	591, 817, 885
Mox20I	TGGCCA	1 cut(s)	899
MroI	TCCGGA	2 cut(s)	490, 985
MroXI	GAANNNNTTC	1 cut(s)	62
MscI	TGGCCA	1 cut(s)	899
MseI	TTAA	4 cut(s)	627, 635, 824, 831
MslI	CAYNNNNRTG	1 cut(s)	786
Msp20I	TGGCCA	1 cut(s)	899
MspA1I	CMGCKG	2 cut(s)	227, 459
MspI	CCGG	2 cut(s)	491, 986
MspR9I	CCNGG	3 cut(s)	729, 765, 816
MvaI	CCWGG	3 cut(s)	729, 765, 816
MvnI	CGCG	1 cut(s)	45
MwoI	GCNNNNNNNGC	4 cut(s)	347, 664, 769, 778
NdeII	GATC	5 cut(s)	157, 201, 694, 724, 921
NheI	GCTAGC	1 cut(s)	422
NlaIII	CATG	6 cut(s)	371, 418, 549, 560, 761, 785
NlaIV	GGNNCC	1 cut(s)	309
NmuCI	GTSAC	2 cut(s)	292, 996
NspI	RCATGY	1 cut(s)	560
PaeI	GCATGC	1 cut(s)	560
PdmI	GAANNNNTTC	1 cut(s)	62
PfeI	GAWTC	1 cut(s)	101
PflMI	CCANNNNNTGG	1 cut(s)	906
PkrI	GCNGC	5 cut(s)	13, 229, 268, 363, 454
Ple19I	CGATCG	1 cut(s)	204
PleI	GAGTC	5 cut(s)	79, 92, 419, 461, 495
PpsI	GAGTC	5 cut(s)	79, 92, 419, 461, 495
PsiI	TTATAA	1 cut(s)	737
Psp1406I	AACGTT	2 cut(s)	638, 834
Psp6I	CCWGG	3 cut(s)	727, 763, 814
PspFI	CCCAGC	1 cut(s)	687
PspGI	CCWGG	3 cut(s)	727, 763, 814
PspN4I	GGNNCC	1 cut(s)	309
PspPI	GGNCC	2 cut(s)	91, 977
PsuI	RGATCY	1 cut(s)	921
PvuI	CGATCG	1 cut(s)	204
PvuII	CAGCTG	1 cut(s)	459
RsaI	GTAC	5 cut(s)	38, 173, 440, 872, 882
RsaNI	GTAC	5 cut(s)	37, 172, 439, 871, 881
RseI	CAYNNNNRTG	1 cut(s)	786
SaqAI	TTAA	4 cut(s)	627, 635, 824, 831
SatI	GCNGC	5 cut(s)	12, 228, 267, 362, 453
Sau3AI	GATC	5 cut(s)	157, 201, 694, 724, 921
Sau96I	GGNCC	2 cut(s)	91, 977
SchI	GAGTC	5 cut(s)	80, 93, 420, 461, 496
ScrFI	CCNGG	3 cut(s)	729, 765, 816
SduI	GDGCHC	2 cut(s)	669, 718
SfaNI	GCATC	2 cut(s)	16, 117
SinI	GGWCC	1 cut(s)	977
SmiMI	CAYNNNNRTG	1 cut(s)	786
SphI	GCATGC	1 cut(s)	560
Sse9I	AATT	5 cut(s)	62, 125, 243, 825, 1040
SsiI	CCGC	7 cut(s)	43, 167, 225, 235, 264, 442, 572
SspI	AATATT	1 cut(s)	22
SspMI	CTAG	1 cut(s)	423
StyD4I	CCNGG	3 cut(s)	727, 763, 814
StyI	CCWWGG	3 cut(s)	564, 905, 1045
TaaI	ACNGT	5 cut(s)	334, 683, 703, 742, 974
TaiI	ACGT	3 cut(s)	582, 641, 837
TaqI	TCGA	3 cut(s)	66, 204, 1021
TasI	AATT	5 cut(s)	62, 125, 243, 825, 1040
TatI	WGTACW	2 cut(s)	870, 880
TfiI	GAWTC	1 cut(s)	101
Tru1I	TTAA	4 cut(s)	627, 635, 824, 831
Tru9I	TTAA	4 cut(s)	627, 635, 824, 831
TscAI	CASTG	2 cut(s)	674, 1036
TseFI	GTSAC	2 cut(s)	292, 996
TseI	GCWGC	5 cut(s)	11, 227, 266, 361, 452
Tsp45I	GTSAC	2 cut(s)	292, 996
TspDTI	ATGAA	2 cut(s)	17, 272
TspGWI	ACGGA	1 cut(s)	916
TspRI	CASTG	2 cut(s)	674, 1036
Van91I	CCANNNNNTGG	1 cut(s)	906
VpaK11BI	GGWCC	1 cut(s)	977
XapI	RAATTY	2 cut(s)	62, 1040
XceI	RCATGY	1 cut(s)	560
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1043
XmnI	GAANNNNTTC	1 cut(s)	62
XspI	CTAG	1 cut(s)	423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.