MD12G1033400.v1.1

pentatricopeptide repeat-containing protein At1g31840

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002491
Physical Location & Seq
Forward (+)
3695816 .. 3699129
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1033400.v1.1.491

Sequence Viewer

Length: 2529 bp
ATGATATCTGCCTCCTCTTCCTCTCCAGTCTCAAAACTACTACTCCTCCTCACGCGGCGGCGCCGCTCTCTCTCTCCCTATTCGACCTTACCCCCGTTCTCTTCCAAAACCCTGATCCACCACATCTCCAACGCCTTCCTCCAAAACAACCCCAAACTCTTAAACCCTCATTTTCTCTCCAAATTACAACCCCACCACCTCCATCCCATCCTCCTCTCCCTTCAATCCAACCCCGTTTCCGCCCTCCGCTTCTTCGACTGGTCCCACGGCTCCCTCGGCCTCCACCACTCCCCGCAGTCCTTCTGTGACCTCATTCACCTGCTGCTCCGCAACCGGATGCTCGCTCCGGCTTCCCGCCTTTTCGACACAATGGTAGGTCAGTTCGGGTCTCAGTTTGACTATTTTGCTGCATTTTCTGATTGTTTTCGGAATCACAGTTCCGATGTTAGTCTTGTTTCTAGCTTTCTGATTGAGAATTTTTGTCGAAATGGGATGTTGGATTCGTCTGTTGATACCTTCATTCGTACGTATAAATTGGGTGTTCGGGTGTCTCCTTATGCGTTGTCTAGAATGCTGAATTGTTTGGTTGATGCGAATCGCGTCGACATGATTCTCGATGCGTATGGAGAAATCTGTAGTGCATTGAGAGGACAACATTTTTGTGCGTATGAGTTTATTATGGTTGGTCTTCTGAACAAGGGTAGATTCGAAACGGGTTTGGAATTTCATTCTGCATTGATTGACAGAGGCTTTTCGCTTGACATTGTTGCTTGTAACAAGATTTTAAAACGTCTTTGTAAAGAAAATCAGGTTGGAGATGGTGAAGATTTCTTTACGGTATTGCTAACTGTTGGTCCGAAGCCAAATGTGGTGACGTTTAGCACGATGATTAACGGGTATTGTAAAGATGGGAAATTGGAAGAGGCAAAGAAGCTTTACAAGATTATGATAGAGAAGGGTATCAGTCCTGACTTGGTTGTATATAGCATCCTAGTTGATGGTCTTTTTAAGGCAGGAAAATTCGAGGACGGGTGTTGGCTTTTCTCAGCGGCGTTGGATGGTGGGATTAAGTTGGATGTAGTCATTTTCAGTTCTGTTATGGATGCGTACGTGAGAATTGGAGATCCAGTGAAGTCAGTGGAGGTCTACCGAAGAATGCTGAAAGAAGGGATCTCACCAAACTCTGTTAGTTACACCATTCTTATTAACGGCATGTGTCAGGATGGTAAGGTCACGGAGGCTTGTGGAATATTTGGTCAGATTTTGAAGTGTGGTTTTGTGCCATCGATTCTGACTTATAGTAGTTTGATTGAAGGAATGTGCAAAATAGGGAATTTAAAAGATGCATTTCAGCTGTATGAAAGTATGATCAAGACGGGCTATGAACCTGATATTATTCTTTACGGTGTGTTATTAAATGGCCTTTGCAAAAATGGGCTGATGGGTGATGCTCTTAGGTTCTTCTTTCAAGCTGTTCATAGGGGCGTTAAGCCAAATGTCTATACTTTCAACATGTTAATAGATGGTTGCTGCAGATTGAAACGATTGAGGGATGCTGTGAAGGTGTACATCCAAATGGGAATTTATAACATAAAACCAGATGTGGTGACGAATACCGTGCTTATTAAGGGTATTTCTGAAGTAGGGAGATTAAACGATGCGTTGGTTTTTTTCTTCCAATCAGTAAAGAAGGGTTTCTTACCAGATGTTGTAACATATTGCACACTGATTGATGGTTGCTGCAAGCAGAAGCATTTACATGCTGGACTCAGGCTATTTGAGATGATGCGAAGAAATGGAGTAAATCCCGATATTGCCATTTATAATGTCCTCATTAATATGCTGTTCAAAGAAAATTGCATAGATGCTGCGCAGGAAGTCTTTGAGCGGTTTGCTGAAAGTGGTCCGGAACCTGATATCATTACATACAACACAATGATTTGTGGTTATTGCTGTCAGAGAAGGTTAGAGGTGGCTGTTCAACTTTTTGAAGAGATGATGCAGGGGAAGTGCAAACCCAATGCCATTACTTGCACTATATTGATTGATGCATTTTGTAAAGAAGGTAAAATGGACGACGCAATGTTGATGTTTGATAAAATGCTGGAAAAGGATCCTGAACCTAACGTGGTCACATATAGTTGTCTGATTGATGGGTTTTTCAAGTCTGAAAACACAAAAAGTGCCTTTGAACTACACGAAGAGATGCTTAAAAGCATCTCTCCAAATATAATCAGTTATAGCATCCTCATTGACGGTCTTTGCAAAAGAGGACTTGTGGAGAAAGCATCCCTTGCATTTCATTGCGCTATAAACAGGGGCCTGGTGCCAGATGTAATAGCATACGGGATTCTGATTCACGGTTACTGCAAGGTAGGAAGAACGGCAGAAGGCCTCGTGTTGTATGGGCGTATGTTAACAAGTGGGATCATGCCGGATGCTGTCATACAAAGGATGATTGCGGAGCATATCCTTGGAGCTGATCGGCGAAAATATGAGGGTACTCCCAACCACATGATTTGCAGATAA

Protein Analysis

843

Amino Acids

94.32

Weight (kDa)

8.25

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 254 - 302 2.3e-10 PPR repeat family
PPR_1 PF12854 285 - 316 1.5e-12 PPR repeat
PPR_2 PF13041 288 - 337 1.9e-17 PPR repeat family
PPR PF01535 291 - 321 1.5e-10 PPR repeat
PPR_3 PF13812 353 - 403 4.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 359 - 407 3.2e-13 PPR repeat family
PPR_1 PF12854 390 - 419 1.5e-09 PPR repeat
PPR_2 PF13041 396 - 442 6.4e-12 PPR repeat family
PPR PF01535 396 - 426 8.5e-06 PPR repeat
PPR_3 PF13812 424 - 473 3.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 425 - 456 1.7e-09 PPR repeat
PPR_2 PF13041 428 - 477 2.8e-13 PPR repeat family
PPR_1 PF12854 460 - 488 1.1e-06 PPR repeat
PPR_2 PF13041 463 - 512 7.2e-15 PPR repeat family
PPR_3 PF13812 491 - 545 1.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 494 - 526 3e-10 PPR repeat
PPR_2 PF13041 502 - 545 8.7e-11 PPR repeat family
PPR_2 PF13041 533 - 582 1.3e-11 PPR repeat family
PPR_3 PF13812 562 - 615 1.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 564 - 597 3.5e-09 PPR repeat
PPR_2 PF13041 568 - 617 2e-13 PPR repeat family
TPR_24 PF23276 571 - 665 2.8e-06 Fungal tetratrico peptide repeats
PPR PF01535 571 - 601 1.2e-06 PPR repeat
PPR_long PF17177 576 - 701 2e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 603 - 651 1.2e-11 PPR repeat family
PPR_3 PF13812 629 - 686 3.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 635 - 666 3.4e-12 PPR repeat
PPR_2 PF13041 638 - 687 1.7e-18 PPR repeat family
PPR PF01535 641 - 668 2.2e-07 PPR repeat
PPR_1 PF12854 671 - 701 2.1e-10 PPR repeat
PPR_2 PF13041 673 - 722 3.9e-16 PPR repeat family
PPR_3 PF13812 673 - 718 8.3e-07 Pentatricopeptide repeat domain
PPR PF01535 676 - 704 1.5e-07 PPR repeat
PPR_3 PF13812 697 - 752 5.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 707 - 736 5.7e-09 PPR repeat
PPR_2 PF13041 708 - 756 2.7e-15 PPR repeat family
PPR_1 PF12854 739 - 764 3.8e-08 PPR repeat
PPR_1 PF12854 773 - 805 1.4e-07 PPR repeat
PPR_2 PF13041 777 - 815 8.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1587, 1824
AarI CACCTGC 1 cut(s) 327
Acc16I TGCGCA 1 cut(s) 1872
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 2 cut(s) 60, 2326
AccBSI CCGCTC 2 cut(s) 66, 1888
AccI GTMKAC 2 cut(s) 603, 1146
AccII CGCG 2 cut(s) 55, 600
AccIII TCCGGA 1 cut(s) 1906
AclWI GGATC 6 cut(s) 109, 1118, 1178, 2108, 2121, 2435
AcsI RAATTY 5 cut(s) 475, 722, 1019, 1333, 1581
AcuI CTGAAG 1 cut(s) 1659
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 4 cut(s) 526, 1109, 1568, 2503
AflIII ACRYGT 1 cut(s) 1512
AjnI CCWGG 1 cut(s) 2322
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AloI GAACNNNNNNTCC 2 cut(s) 421, 453
AluBI AGCT 5 cut(s) 462, 934, 1354, 1472, 2480
AluI AGCT 5 cut(s) 462, 934, 1354, 1472, 2480
Alw26I GTCTC 3 cut(s) 34, 393, 555
AlwI GGATC 6 cut(s) 109, 1118, 1178, 2108, 2121, 2435
Aor13HI TCCGGA 1 cut(s) 1906
AoxI GGCC 4 cut(s) 277, 1420, 2320, 2392
ApeKI GCWGC 5 cut(s) 322, 407, 1530, 1740, 1868
ApoI RAATTY 5 cut(s) 475, 722, 1019, 1333, 1581
ArsI GACNNNNNNTTYG 2 cut(s) 642, 674
AseI ATTAAT 1 cut(s) 1836
Asp700I GAANNNNTTC 1 cut(s) 1694
AspLEI GCGC 3 cut(s) 63, 1873, 2309
AspS9I GGNCC 4 cut(s) 261, 854, 1904, 2320
AsuHPI GGTGA 6 cut(s) 308, 833, 883, 1167, 1457, 1618
AsuII TTCGAA 1 cut(s) 708
AvaII GGWCC 3 cut(s) 261, 854, 1904
BamHI GGATCC 1 cut(s) 2113
BanI GGYRCC 2 cut(s) 60, 2326
BauI CACGAG 1 cut(s) 2396
BbsI GAAGAC 1 cut(s) 680
BbvI GCAGC 5 cut(s) 309, 394, 1517, 1727, 1855
BceAI ACGGC 3 cut(s) 283, 1225, 2400
BcgI CGANNNNNNTGC 2 cut(s) 1600, 1634
BciT130I CCWGG 1 cut(s) 2324
BclI TGATCA 1 cut(s) 1368
BcoDI GTCTC 3 cut(s) 34, 393, 555
BfaI CTAG 3 cut(s) 459, 567, 992
BfmI CTRYAG 2 cut(s) 634, 1531
BfoI RGCGCY 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 327
BisI GCNGC 9 cut(s) 56, 59, 64, 323, 408, 1050, 1531, 1741, 1869
BlsI GCNGC 9 cut(s) 57, 60, 65, 324, 409, 1051, 1532, 1742, 1870
Bme1390I CCNGG 1 cut(s) 2324
Bme18I GGWCC 3 cut(s) 261, 854, 1904
BmgT120I GGNCC 4 cut(s) 261, 854, 1904, 2320
BmiI GGNNCC 7 cut(s) 62, 263, 271, 1911, 2115, 2321, 2328
BmrFI CCNGG 1 cut(s) 2324
BpiI GAAGAC 1 cut(s) 680
BpmI CTGGAG 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 708
Bsa29I ATCGAT 1 cut(s) 1286
BsaAI YACGTR 2 cut(s) 528, 1111
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 61
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 265, 274, 2473
BsaWI WCCGGW 2 cut(s) 333, 1906
BsaXI ACNNNNNCTCC 8 cut(s) 27, 30, 57, 60, 110, 140, 1113, 1143
Bse1I ACTGG 3 cut(s) 26, 263, 1127
Bse3DI GCAATG 2 cut(s) 2088, 2302
Bse8I GATNNNNATC 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 1906
BseBI CCWGG 1 cut(s) 2324
BseCI ATCGAT 1 cut(s) 1286
BseDI CCNNGG 3 cut(s) 265, 274, 2473
BseJI GATNNNNATC 1 cut(s) 594
BseMI GCAATG 2 cut(s) 2088, 2302
BseMII CTCAG 3 cut(s) 404, 1059, 1783
BseNI ACTGG 3 cut(s) 26, 263, 1127
BseRI GAGGAG 4 cut(s) 4, 35, 38, 203
BseXI GCAGC 5 cut(s) 309, 394, 1517, 1727, 1855
Bsh1236I CGCG 2 cut(s) 55, 600
BshFI GGCC 4 cut(s) 279, 1422, 2322, 2394
BshNI GGYRCC 2 cut(s) 60, 2326
BshVI ATCGAT 1 cut(s) 1286
BsiSI CCGG 4 cut(s) 334, 347, 1907, 2435
BsiWI CGTACG 2 cut(s) 524, 1107
BslFI GGGAC 1 cut(s) 247
BsmAI GTCTC 3 cut(s) 34, 393, 555
BsmFI GGGAC 1 cut(s) 247
BsmI GAATGC 2 cut(s) 576, 1161
BsnI GGCC 4 cut(s) 279, 1422, 2322, 2394
Bso31I GGTCTC 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 708
Bsp13I TCCGGA 1 cut(s) 1906
Bsp1407I TGTACA 1 cut(s) 1566
Bsp143I GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
BspANI GGCC 4 cut(s) 279, 1422, 2322, 2394
BspCNI CTCAG 3 cut(s) 403, 1058, 1782
BspDI ATCGAT 1 cut(s) 1286
BspEI TCCGGA 1 cut(s) 1906
BspFNI CGCG 2 cut(s) 55, 600
BspLI GGNNCC 7 cut(s) 62, 263, 271, 1911, 2115, 2321, 2328
BspMAI CTGCAG 1 cut(s) 1535
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 6 cut(s) 109, 1118, 1178, 2108, 2121, 2435
BspT104I TTCGAA 1 cut(s) 708
BspT107I GGYRCC 2 cut(s) 60, 2326
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 2 cut(s) 66, 1888
BsrDI GCAATG 2 cut(s) 2088, 2302
BsrGI TGTACA 1 cut(s) 1566
BsrI ACTGG 3 cut(s) 26, 263, 1127
BssECI CCNNGG 3 cut(s) 265, 274, 2473
BssMI GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
BssNI GRCGYC 1 cut(s) 61
BssSI CACGAG 1 cut(s) 2396
BssT1I CCWWGG 1 cut(s) 2473
Bst2BI CACGAG 1 cut(s) 2396
Bst2UI CCWGG 1 cut(s) 2324
Bst4CI ACNGT 7 cut(s) 437, 838, 850, 1406, 1618, 2258, 2363
Bst6I CTCTTC 5 cut(s) 22, 106, 915, 1986, 2196
BstACI GRCGYC 1 cut(s) 61
BstAPI GCANNNNNTGC 1 cut(s) 2294
BstAUI TGTACA 1 cut(s) 1566
BstBAI YACGTR 2 cut(s) 528, 1111
BstBI TTCGAA 1 cut(s) 708
BstC8I GCNNGC 2 cut(s) 342, 1745
BstDEI CTNAG 4 cut(s) 390, 1045, 1454, 1769
BstDSI CCRYGG 1 cut(s) 265
BstFNI CGCG 2 cut(s) 55, 600
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 3 cut(s) 63, 1873, 2309
BstKTI GATC 7 cut(s) 117, 1126, 1173, 1371, 2116, 2430, 2485
BstMAI GTCTC 3 cut(s) 34, 393, 555
BstMBI GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
BstMWI GCNNNNNNNGC 2 cut(s) 276, 2294
BstNI CCWGG 1 cut(s) 2324
BstNSI RCATGY 3 cut(s) 1216, 1516, 1763
BstSCI CCNGG 1 cut(s) 2322
BstSFI CTRYAG 2 cut(s) 634, 1531
BstSNI TACGTA 1 cut(s) 528
BstUI CGCG 2 cut(s) 55, 600
BstV1I GCAGC 5 cut(s) 309, 394, 1517, 1727, 1855
BstV2I GAAGAC 1 cut(s) 680
BstX2I RGATCY 3 cut(s) 1123, 1170, 2113
BstYI RGATCY 3 cut(s) 1123, 1170, 2113
Bsu15I ATCGAT 1 cut(s) 1286
BsuRI GGCC 4 cut(s) 279, 1422, 2322, 2394
BsuTUI ATCGAT 1 cut(s) 1286
BtgI CCRYGG 1 cut(s) 265
BtsIMutI CAGTG 3 cut(s) 1134, 1143, 1724
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 2 cut(s) 342, 1745
CfoI GCGC 3 cut(s) 63, 1873, 2309
Cfr13I GGNCC 4 cut(s) 261, 854, 1904, 2320
ClaI ATCGAT 1 cut(s) 1286
CseI GACGC 2 cut(s) 589, 2087
Csp6I GTAC 4 cut(s) 525, 1108, 1567, 2502
CspCI CAANNNNNGTGG 1 cut(s) 2501
CviAII CATG 6 cut(s) 607, 1213, 1513, 1760, 2431, 2515
CviQI GTAC 4 cut(s) 525, 1108, 1567, 2502
DdeI CTNAG 4 cut(s) 390, 1045, 1454, 1769
DinI GGCGCC 1 cut(s) 62
DpnI GATC 7 cut(s) 116, 1125, 1172, 1370, 2115, 2429, 2484
DpnII GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
DraI TTTAAA 2 cut(s) 786, 1338
Eam1104I CTCTTC 5 cut(s) 22, 106, 915, 1986, 2196
EarI CTCTTC 5 cut(s) 22, 106, 915, 1986, 2196
EciI GGCGGA 1 cut(s) 229
Eco105I TACGTA 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 2473
Eco147I AGGCCT 1 cut(s) 2394
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 2 cut(s) 6, 1918
Eco47I GGWCC 3 cut(s) 261, 854, 1904
Eco57I CTGAAG 1 cut(s) 1659
EcoO109I RGGNCCY 1 cut(s) 2320
EcoRII CCWGG 1 cut(s) 2322
EcoRV GATATC 2 cut(s) 6, 1918
EcoT14I CCWWGG 1 cut(s) 2473
EcoT22I ATGCAT 2 cut(s) 1348, 2053
EgeI GGCGCC 1 cut(s) 62
EheI GGCGCC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 2473
FaeI CATG 6 cut(s) 610, 1216, 1516, 1763, 2434, 2518
FalI AAGNNNNNCTT 6 cut(s) 1682, 1714, 2193, 2225, 2277, 2309
FaqI GGGAC 1 cut(s) 247
FatI CATG 6 cut(s) 606, 1212, 1512, 1759, 2430, 2514
FauI CCCGC 2 cut(s) 300, 362
FbaI TGATCA 1 cut(s) 1368
FblI GTMKAC 2 cut(s) 603, 1146
Fnu4HI GCNGC 9 cut(s) 56, 59, 64, 323, 408, 1050, 1531, 1741, 1869
Fsp4HI GCNGC 9 cut(s) 56, 59, 64, 323, 408, 1050, 1531, 1741, 1869
FspBI CTAG 3 cut(s) 459, 567, 992
FspI TGCGCA 1 cut(s) 1872
GlaI GCGC 3 cut(s) 62, 1872, 2308
GluI GCNGC 9 cut(s) 56, 59, 64, 323, 408, 1050, 1531, 1741, 1869
GsuI CTGGAG 1 cut(s) 9
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 4 cut(s) 279, 1422, 2322, 2394
HapII CCGG 4 cut(s) 334, 347, 1907, 2435
HgaI GACGC 2 cut(s) 589, 2087
HhaI GCGC 3 cut(s) 63, 1873, 2309
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 6 cut(s) 610, 1216, 1516, 1763, 2434, 2518
Hin6I GCGC 3 cut(s) 61, 1871, 2307
HinP1I GCGC 3 cut(s) 61, 1871, 2307
HincII GTYRAC 2 cut(s) 604, 2418
HindII GTYRAC 2 cut(s) 604, 2418
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 9 cut(s) 430, 500, 595, 610, 705, 1288, 1767, 2350, 2356
HpaI GTTAAC 1 cut(s) 2418
HpaII CCGG 4 cut(s) 334, 347, 1907, 2435
HphI GGTGA 6 cut(s) 308, 833, 883, 1167, 1457, 1618
Hpy166II GTNNAC 4 cut(s) 604, 1147, 1567, 2418
Hpy188III TCNNGA 8 cut(s) 567, 614, 968, 1220, 1372, 1808, 1907, 2117
Hpy8I GTNNAC 4 cut(s) 604, 1147, 1567, 2418
Hpy99I CGWCG 2 cut(s) 605, 2081
HpyCH4III ACNGT 7 cut(s) 437, 838, 850, 1406, 1618, 2258, 2363
HpyCH4IV ACGT 5 cut(s) 527, 790, 875, 1110, 2127
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 2294
HpyF3I CTNAG 4 cut(s) 390, 1045, 1454, 1769
HpySE526I ACGT 5 cut(s) 527, 790, 875, 1110, 2127
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 6 cut(s) 610, 1216, 1516, 1763, 2434, 2518
HspAI GCGC 3 cut(s) 61, 1871, 2307
KasI GGCGCC 1 cut(s) 60
Kpn2I TCCGGA 1 cut(s) 1906
Ksp22I TGATCA 1 cut(s) 1368
KspAI GTTAAC 1 cut(s) 2418
Kzo9I GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
LmnI GCTCC 5 cut(s) 275, 330, 349, 2464, 2477
Lsp1109I GCAGC 5 cut(s) 309, 394, 1517, 1727, 1855
MaeI CTAG 3 cut(s) 459, 567, 992
MaeII ACGT 5 cut(s) 527, 790, 875, 1110, 2127
MaeIII GTNAC 9 cut(s) 305, 773, 871, 1190, 1231, 1606, 1711, 2131, 2363
MalI GATC 7 cut(s) 116, 1125, 1172, 1370, 2115, 2429, 2484
MbiI CCGCTC 2 cut(s) 66, 1888
MboI GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
MflI RGATCY 3 cut(s) 1123, 1170, 2113
Mly113I GGCGCC 1 cut(s) 61
MlyI GAGTC 1 cut(s) 1761
MmeI TCCRAC 6 cut(s) 153, 252, 477, 793, 1035, 1053
Mph1103I ATGCAT 2 cut(s) 1348, 2053
MroI TCCGGA 1 cut(s) 1906
MroXI GAANNNNTTC 1 cut(s) 1694
MslI CAYNNNNRTG 4 cut(s) 660, 1574, 1758, 1838
MspA1I CMGCKG 2 cut(s) 1049, 1354
MspI CCGG 4 cut(s) 334, 347, 1907, 2435
MspR9I CCNGG 1 cut(s) 2324
Mva1269I GAATGC 2 cut(s) 576, 1161
MvaI CCWGG 1 cut(s) 2324
MvnI CGCG 2 cut(s) 55, 600
MwoI GCNNNNNNNGC 2 cut(s) 276, 2294
NarI GGCGCC 1 cut(s) 61
NdeII GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
NlaIII CATG 6 cut(s) 610, 1216, 1516, 1763, 2434, 2518
NlaIV GGNNCC 7 cut(s) 62, 263, 271, 1911, 2115, 2321, 2328
NmeAIII GCCGAG 1 cut(s) 255
NmuCI GTSAC 5 cut(s) 305, 871, 1231, 1606, 2131
NsbI TGCGCA 1 cut(s) 1872
NsiI ATGCAT 2 cut(s) 1348, 2053
NspI RCATGY 3 cut(s) 1216, 1516, 1763
NspV TTCGAA 1 cut(s) 708
PaqCI CACCTGC 1 cut(s) 327
PceI AGGCCT 1 cut(s) 2394
PciI ACATGT 1 cut(s) 1512
PcsI WCGNNNNNNNCGW 1 cut(s) 881
PctI GAATGC 2 cut(s) 576, 1161
PdmI GAANNNNTTC 1 cut(s) 1694
PfeI GAWTC 8 cut(s) 430, 500, 595, 610, 705, 1288, 2350, 2356
Pfl23II CGTACG 2 cut(s) 524, 1107
PkrI GCNGC 9 cut(s) 57, 60, 65, 324, 409, 1051, 1532, 1742, 1870
PleI GAGTC 1 cut(s) 1761
PluTI GGCGCC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 1761
Ppu21I YACGTR 2 cut(s) 528, 1111
PscI ACATGT 1 cut(s) 1512
PshBI ATTAAT 1 cut(s) 1836
PsiI TTATAA 2 cut(s) 1587, 1824
Psp6I CCWGG 1 cut(s) 2322
PspGI CCWGG 1 cut(s) 2322
PspLI CGTACG 2 cut(s) 524, 1107
PspN4I GGNNCC 7 cut(s) 62, 263, 271, 1911, 2115, 2321, 2328
PspPI GGNCC 4 cut(s) 261, 854, 1904, 2320
PstI CTGCAG 1 cut(s) 1535
PsuI RGATCY 3 cut(s) 1123, 1170, 2113
PvuII CAGCTG 1 cut(s) 1354
RsaI GTAC 4 cut(s) 526, 1109, 1568, 2503
RsaNI GTAC 4 cut(s) 525, 1108, 1567, 2502
RseI CAYNNNNRTG 4 cut(s) 660, 1574, 1758, 1838
SalI GTCGAC 1 cut(s) 602
SatI GCNGC 9 cut(s) 56, 59, 64, 323, 408, 1050, 1531, 1741, 1869
Sau3AI GATC 7 cut(s) 114, 1123, 1170, 1368, 2113, 2427, 2482
Sau96I GGNCC 4 cut(s) 261, 854, 1904, 2320
SchI GAGTC 1 cut(s) 1761
ScrFI CCNGG 1 cut(s) 2324
SfcI CTRYAG 2 cut(s) 634, 1531
SfoI GGCGCC 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 708
SinI GGWCC 3 cut(s) 261, 854, 1904
SmiMI CAYNNNNRTG 4 cut(s) 660, 1574, 1758, 1838
SnaBI TACGTA 1 cut(s) 528
SseBI AGGCCT 1 cut(s) 2394
SspDI GGCGCC 1 cut(s) 60
SspI AATATT 1 cut(s) 1251
SspMI CTAG 3 cut(s) 459, 567, 992
StuI AGGCCT 1 cut(s) 2394
StyD4I CCNGG 1 cut(s) 2322
StyI CCWWGG 1 cut(s) 2473
TaaI ACNGT 7 cut(s) 437, 838, 850, 1406, 1618, 2258, 2363
TaiI ACGT 5 cut(s) 530, 793, 878, 1113, 2130
TaqI TCGA 9 cut(s) 83, 255, 363, 484, 603, 615, 708, 1023, 1286
TatI WGTACW 1 cut(s) 1566
TauI GCSGC 4 cut(s) 58, 61, 66, 1052
TfiI GAWTC 8 cut(s) 430, 500, 595, 610, 705, 1288, 2350, 2356
TscAI CASTG 3 cut(s) 1134, 1143, 1731
TseFI GTSAC 5 cut(s) 305, 871, 1231, 1606, 2131
TseI GCWGC 5 cut(s) 322, 407, 1530, 1740, 1868
Tsp45I GTSAC 5 cut(s) 305, 871, 1231, 1606, 2131
TspDTI ATGAA 6 cut(s) 508, 716, 1374, 1398, 1466, 2291
TspGWI ACGGA 1 cut(s) 1250
TspRI CASTG 3 cut(s) 1134, 1143, 1731
VpaK11BI GGWCC 3 cut(s) 261, 854, 1904
VspI ATTAAT 1 cut(s) 1836
XapI RAATTY 5 cut(s) 475, 722, 1019, 1333, 1581
XbaI TCTAGA 1 cut(s) 566
XceI RCATGY 3 cut(s) 1216, 1516, 1763
XmiI GTMKAC 2 cut(s) 603, 1146
XmnI GAANNNNTTC 1 cut(s) 1694
XspI CTAG 3 cut(s) 459, 567, 992
Zsp2I ATGCAT 2 cut(s) 1348, 2053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.