Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0002491

Physical Location & Seq

Genomic Coordinates Forward (+)

2786220 .. 2789031

Gene Structure 2812 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g03070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2562 bp

ATGATATCTGCCTCCTCTTCCTCTCCAGTCTCAAAACTACTACTCCTCCTCACGCGGCGGTGCCGCTCTCTCTCTCCCTATTCTACTTTACCCCCGTTCTCTTCCAAAACCCTGATCCACCACATCTCCAACGCCTTCCTCCAAAACAACCCCAAACTCTTAAACCCTCATTTTCTCTCCAAATTACAACCCCACCACCTCCACCCCATCCTTCTCTCCCTTCAATCCAACCCCGTTTCGGCCCTCCGCTTCTTCGACTGGTCCCACGGCTCCCTCAGCCTCCACCACTCCCCGCAGTCCTTCTGTGACCTCATTCACCTGCTGCTCCGCCACCGGATGCTCGCTCCGGCTTCCCGCCTTTTCGACACAATGGTAGGTCAGTTCGGGTCTCAGTTTAACTATTTTGCTGCATTTTCTGATTGTTTTCGGAATCACAGTTCCGATGTTAGTCTTGTTTGTAGCTTTCTGATTGAGAATTTTTGTCGAAATGGGATGTTGGATTCGTCTGTTGATACCTTCATTCGTATGTATAAATTGGGTGTTCCGGTGTCTCCTTATGCGTTGTCTAGAATGCTGAATTGTTTGGTTGATGCGAATCACGTCGACATGATTCTCGATGCATATGGAGAAATCTGTAGTGCATTGAGAGGACAACAATTTTGTGTGTATGAGTTTGTTATGGTTGGTCTTCTGAACAAGGGTAGATTCGAAACGGGTTTGGAATTTCATTCTGCATTGATTGACAGAGGCTTTTCGCTTGACATTGTTGCTTGTAACAAGATTTTAAAACGTCTTTGTAAAGAAAATCAGGTTGGAGATGGTGAAGATTTCTTTACGGTATTGCTAACAGTTGGTCCGAAGCCAAATGTGGTGACGTTTAGCACGATGATTAACAGGTATTGTAAAGATGGGAAATTGGAAGAGGCAAAGAAGCTTTACAAGATTATGATAGAGAAGGGTATCGGTCCTGACTTGGTTGTATATAGTATCCTAGTTGATGGTTTTTTTAAGGCAGGGAAATTCGAGGAAGGGTGTTGGCTTTTTTCAGCGGCGTTGGATTGCGGGATTAAGTTGGATGTAGTCATTTTCAGTTCTGTTATGGATGCTTACGTGAGAATTGGAGATCTGGTGAAGTCAGTGGAGGTCTACCGAAGAATGCTGAAAGAAGGGATCTCACCAAACTCTGTTAGTTACACCATTCTTATTAACAGCATGTGTCAGGATGGTAAGGTTGCGGAGGCTTGTGGAATATTTGGTCGGATTTTGAAATGTGGTTTTGTGCAAAATAGGGAATTTAAAAGATGCATTTCAGCTGTACGAGAGTATGATCTAATCCTGATGGGCTATGAACCTGATATTATTCTTTACGGTGTGTTATTAAATGGCCTTTGCAAAAATGGGCTGATGGGTGATGCTCTTAGGTTCTTCTTTCAAGCTGTTCATAGGGGCGTTAAGCCAAATGTCTATACTTTCAACATGTTAATAGATGGTTGCTGCAGATTGAAACGATTGAGGGATGCTGTGAAGGTGTACATCCAAATGGGAATTTATAACATAAAACCAGATGTGGTGACGAATACCGTGCTTATTAAGGGTATTTCTGAAGTAGGGAGATTAAACGATGCGTTGGTGTTTTTCTTCCAATCAGTAAAGAAGGGTTTCTTACCTGATGTTGTAACATATTGCACACTGATTGATGGTTGCTGCAAGCAGAAACATTTACATGCCGGACTCTGGCTATTTGAGATGATGCAAAGAAATGGAGTAAATCCCGATATTGCCATTTATAATGTCCTCATCAATATGCTGTTCAAAGAAAATTGCATAGATGCTGCGCAGGAAGTCTTTGAGCGGTTTGCTGAAAGTGGTCCTGAACCTGATATCATTACATACAACACAATGATTTGTGGTTATTGCTTTCAGAGAAGGTTAGAGGTGGCTGTTCAACTTTTTGAAGAGATGATGCAGGGGCAGTGCAAACCCAATGCCATTACTTGCACTATATTGATTGATGCATTTTGTAAAGAAGGTAAAATGGACGATGCGATGTTGATGTTCGATAAAATGCATGAAAGGGATCCTGAACCGAACGTGGTCACATATAGTTGTCTGATTGACGGGTTTTTCAAGTCTGAAAACACAAAAAGTGCCTTTGAACTACACGAAGAGATGCTTAAAAGCGTCTCTCCAAATATAGTCAGTTATAGCATCCTCATTGATGGTCTTTGTAAAAGAGGACTTGTGGAGAAAGCATCCCTTGCATTTCATTGTGCTATAAACAGGGGCTTGGTGCCCGATGTAATAGCATACGGGATTCTGATTCGCGGTTACTGCAAGGTAGGAAGAACCGCAGAAGGCCTCGTGTTGTATGGGCGTATGTTAACAAGCGGGATCATTCCGGATGCTGTCATACAAAGGACGATTGCGGAGCATATCCTTGAAACTGATCGGCGAAAATATGAGAGATATTGCAGGTTTCTCCGGGATGTAAGAACTCATTTTCCCTTGGTACGCATATTGTATCCAAGCTCAGCACCCGGCAGCCAAGTATCCGTCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

854

Amino Acids

96.29

Weight (kDa)

8.37

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	254 - 302	4.1e-09	PPR repeat family
PPR_1	PF12854	285 - 316	5.4e-11	PPR repeat
PPR_2	PF13041	288 - 337	1.9e-15	PPR repeat family
PPR	PF01535	291 - 321	3.4e-09	PPR repeat
PPR_3	PF13812	353 - 404	1.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	359 - 407	1.8e-12	PPR repeat family
PPR_1	PF12854	390 - 418	4.7e-09	PPR repeat
PPR_1	PF12854	447 - 476	6e-07	PPR repeat
PPR_2	PF13041	451 - 500	7.3e-15	PPR repeat family
PPR_3	PF13812	479 - 533	1.3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	482 - 514	3e-10	PPR repeat
PPR_2	PF13041	492 - 533	2.1e-09	PPR repeat family
PPR_2	PF13041	527 - 570	8.4e-09	PPR repeat family
PPR_3	PF13812	550 - 603	3e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	552 - 584	1.9e-07	PPR repeat
PPR_2	PF13041	556 - 593	6.5e-09	PPR repeat family
PPR	PF01535	559 - 589	1.5e-06	PPR repeat
PPR_long	PF17177	564 - 690	7.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	591 - 639	1.3e-11	PPR repeat family
PPR_3	PF13812	617 - 674	1.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	623 - 654	2e-11	PPR repeat
PPR_2	PF13041	626 - 675	4.9e-19	PPR repeat family
PPR	PF01535	629 - 656	7.4e-07	PPR repeat
PPR_1	PF12854	659 - 689	1e-10	PPR repeat
PPR_3	PF13812	661 - 706	6.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	664 - 710	6e-15	PPR repeat family
PPR	PF01535	664 - 692	1.8e-07	PPR repeat
PPR_3	PF13812	685 - 740	4.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	695 - 724	6.3e-09	PPR repeat
PPR_2	PF13041	696 - 744	1.5e-15	PPR repeat family
PPR_1	PF12854	727 - 752	1.8e-08	PPR repeat
PPR_1	PF12854	761 - 793	5.7e-07	PPR repeat
PPR_2	PF13041	765 - 803	3.2e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31840 AT1G31840
malus_domestica	MD12G1033400.v1.1
prunus_persica	Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1
pyrus_communis	pycom12g03070
rosa_chinensis	RchiOBHm_Chr2g0139321 RchiOBHm_Chr7g0227311
rosa_laevigata	RLG00000001660
rosa_multiflora	Rmu_sc0001246.1_g000012 Rmu_sc0002525.1_g000013 Rmu_sc0003153.1_g000006 Rmu_sc0011922.1_g000005
rosa_roxburghii	Rroxscaffold_3G00232240
rosa_rugosa	Rorug07G0241200
rosa_samantha	Rh2BG417100 Rh7AG391100 Rh7AG391600 Rh7AG392200 Rh7AG392500 Rh7BG376100 Rh7CG192200 Rh7CG410100 Rh7CG410500 Rh7CG411300 Rh7CG411800 Rh7DG388700
rosa_wichuraiana	Rw7G032710 Rw7G032740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1551, 1788
AarI	CACCTGC	1 cut(s)	327
Acc16I	TGCGCA	1 cut(s)	1836
Acc36I	ACCTGC	2 cut(s)	327, 2463
AccB1I	GGYRCC	2 cut(s)	60, 2290
AccBSI	CCGCTC	2 cut(s)	66, 1852
AccI	GTMKAC	2 cut(s)	603, 1146
AccII	CGCG	2 cut(s)	55, 2325
AccIII	TCCGGA	1 cut(s)	2398
AclWI	GGATC	5 cut(s)	109, 1178, 2072, 2085, 2399
AcsI	RAATTY	5 cut(s)	475, 722, 1019, 1292, 1545
AcuI	CTGAAG	1 cut(s)	1623
AfaI	GTAC	3 cut(s)	1317, 1532, 2511
AfiI	CCNNNNNNNGG	2 cut(s)	238, 1734
AflIII	ACRYGT	1 cut(s)	1476
AjiI	CACGTC	1 cut(s)	601
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
AloI	GAACNNNNNNTCC	4 cut(s)	421, 453, 2485, 2517
AluBI	AGCT	5 cut(s)	462, 934, 1313, 1436, 2529
AluI	AGCT	5 cut(s)	462, 934, 1313, 1436, 2529
Alw26I	GTCTC	4 cut(s)	34, 393, 555, 2188
AlwI	GGATC	5 cut(s)	109, 1178, 2072, 2085, 2399
Aor13HI	TCCGGA	1 cut(s)	2398
AoxI	GGCC	3 cut(s)	240, 1384, 2356
ApeKI	GCWGC	6 cut(s)	322, 407, 1494, 1704, 1832, 2541
ApoI	RAATTY	5 cut(s)	475, 722, 1019, 1292, 1545
ArsI	GACNNNNNNTTYG	2 cut(s)	642, 674
Asp700I	GAANNNNTTC	1 cut(s)	1658
AspLEI	GCGC	1 cut(s)	1837
AspS9I	GGNCC	5 cut(s)	241, 261, 854, 965, 1868
AsuC2I	CCSGG	2 cut(s)	2483, 2538
AsuHPI	GGTGA	7 cut(s)	308, 833, 883, 1141, 1167, 1421, 1582
AsuII	TTCGAA	1 cut(s)	708
AvaII	GGWCC	4 cut(s)	261, 854, 965, 1868
BaeGI	GKGCMC	1 cut(s)	2295
BamHI	GGATCC	1 cut(s)	2077
BanI	GGYRCC	2 cut(s)	60, 2290
BauI	CACGAG	1 cut(s)	2360
BbsI	GAAGAC	1 cut(s)	680
BbvCI	CCTCAGC	1 cut(s)	275
BbvI	GCAGC	6 cut(s)	309, 394, 1481, 1691, 1819, 2553
BceAI	ACGGC	1 cut(s)	283
BcgI	CGANNNNNNTGC	2 cut(s)	1564, 1598
BciVI	GTATCC	3 cut(s)	998, 2532, 2560
BcnI	CCSGG	2 cut(s)	2483, 2538
BcoDI	GTCTC	4 cut(s)	34, 393, 555, 2188
BfaI	CTAG	2 cut(s)	567, 992
BfmI	CTRYAG	2 cut(s)	634, 1495
BfuAI	ACCTGC	2 cut(s)	327, 2463
BfuI	GTATCC	3 cut(s)	998, 2532, 2560
BglII	AGATCT	1 cut(s)	1123
BisI	GCNGC	9 cut(s)	56, 64, 323, 408, 1050, 1495, 1705, 1833, 2542
BlpI	GCTNAGC	1 cut(s)	2530
BlsI	GCNGC	9 cut(s)	57, 65, 324, 409, 1051, 1496, 1706, 1834, 2543
Bme1390I	CCNGG	2 cut(s)	2483, 2538
Bme18I	GGWCC	4 cut(s)	261, 854, 965, 1868
BmgBI	CACGTC	1 cut(s)	601
BmgT120I	GGNCC	5 cut(s)	241, 261, 854, 965, 1868
BmiI	GGNNCC	5 cut(s)	62, 263, 271, 2079, 2292
BmrFI	CCNGG	2 cut(s)	2483, 2538
BpiI	GAAGAC	1 cut(s)	680
BpmI	CTGGAG	1 cut(s)	9
Bpu10I	CCTNAGC	1 cut(s)	275
Bpu1102I	GCTNAGC	1 cut(s)	2530
Bpu14I	TTCGAA	1 cut(s)	708
BpuMI	CCSGG	2 cut(s)	2483, 2538
BsaAI	YACGTR	1 cut(s)	1111
BsaBI	GATNNNNATC	1 cut(s)	594
BsaI	GGTCTC	1 cut(s)	393
BsaJI	CCNNGG	2 cut(s)	265, 2505
BsaWI	WCCGGW	3 cut(s)	333, 544, 2398
BsaXI	ACNNNNNCTCC	8 cut(s)	27, 30, 57, 60, 110, 140, 1113, 1143
Bsc4I	CCNNNNNNNGG	2 cut(s)	238, 1734
Bse1I	ACTGG	2 cut(s)	26, 263
Bse8I	GATNNNNATC	1 cut(s)	594
BseAI	TCCGGA	1 cut(s)	2398
BseDI	CCNNGG	2 cut(s)	265, 2505
BseJI	GATNNNNATC	1 cut(s)	594
BseLI	CCNNNNNNNGG	2 cut(s)	238, 1734
BseMII	CTCAG	3 cut(s)	289, 404, 2544
BseNI	ACTGG	2 cut(s)	26, 263
BseRI	GAGGAG	3 cut(s)	4, 35, 38
BseSI	GKGCMC	1 cut(s)	2295
BseXI	GCAGC	6 cut(s)	309, 394, 1481, 1691, 1819, 2553
Bsh1236I	CGCG	2 cut(s)	55, 2325
BshFI	GGCC	3 cut(s)	242, 1386, 2358
BshNI	GGYRCC	2 cut(s)	60, 2290
BsiSI	CCGG	7 cut(s)	334, 347, 545, 1728, 2399, 2482, 2538
BslFI	GGGAC	1 cut(s)	247
BslI	CCNNNNNNNGG	2 cut(s)	238, 1734
BsmAI	GTCTC	4 cut(s)	34, 393, 555, 2188
BsmBI	CGTCTC	1 cut(s)	2188
BsmFI	GGGAC	1 cut(s)	247
BsmI	GAATGC	2 cut(s)	576, 1161
BsnI	GGCC	3 cut(s)	242, 1386, 2358
Bso31I	GGTCTC	1 cut(s)	393
Bsp119I	TTCGAA	1 cut(s)	708
Bsp1286I	GDGCHC	1 cut(s)	2295
Bsp13I	TCCGGA	1 cut(s)	2398
Bsp1407I	TGTACA	1 cut(s)	1530
Bsp143I	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
Bsp1720I	GCTNAGC	1 cut(s)	2530
BspANI	GGCC	3 cut(s)	242, 1386, 2358
BspCNI	CTCAG	3 cut(s)	288, 403, 2543
BspEI	TCCGGA	1 cut(s)	2398
BspFNI	CGCG	2 cut(s)	55, 2325
BspLI	GGNNCC	5 cut(s)	62, 263, 271, 2079, 2292
BspMAI	CTGCAG	1 cut(s)	1499
BspMI	ACCTGC	2 cut(s)	327, 2463
BspPI	GGATC	5 cut(s)	109, 1178, 2072, 2085, 2399
BspT104I	TTCGAA	1 cut(s)	708
BspT107I	GGYRCC	2 cut(s)	60, 2290
BspTNI	GGTCTC	1 cut(s)	393
BsrBI	CCGCTC	2 cut(s)	66, 1852
BsrGI	TGTACA	1 cut(s)	1530
BsrI	ACTGG	2 cut(s)	26, 263
BssECI	CCNNGG	2 cut(s)	265, 2505
BssMI	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
BssSI	CACGAG	1 cut(s)	2360
BssT1I	CCWWGG	1 cut(s)	2505
Bst2BI	CACGAG	1 cut(s)	2360
Bst4CI	ACNGT	5 cut(s)	437, 838, 850, 1370, 1582
Bst6I	CTCTTC	5 cut(s)	22, 106, 915, 1950, 2160
BstAPI	GCANNNNNTGC	1 cut(s)	2258
BstAUI	TGTACA	1 cut(s)	1530
BstBAI	YACGTR	1 cut(s)	1111
BstBI	TTCGAA	1 cut(s)	708
BstC8I	GCNNGC	2 cut(s)	342, 1709
BstDEI	CTNAG	4 cut(s)	275, 390, 1418, 2530
BstDSI	CCRYGG	1 cut(s)	265
BstFNI	CGCG	2 cut(s)	55, 2325
BstHHI	GCGC	1 cut(s)	1837
BstKTI	GATC	7 cut(s)	117, 1126, 1173, 1330, 2080, 2394, 2449
BstMAI	GTCTC	4 cut(s)	34, 393, 555, 2188
BstMBI	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
BstMWI	GCNNNNNNNGC	3 cut(s)	276, 2258, 2331
BstNSI	RCATGY	3 cut(s)	1216, 1480, 1727
BstSCI	CCNGG	2 cut(s)	2481, 2536
BstSFI	CTRYAG	2 cut(s)	634, 1495
BstSLI	GKGCMC	1 cut(s)	2295
BstUI	CGCG	2 cut(s)	55, 2325
BstV1I	GCAGC	6 cut(s)	309, 394, 1481, 1691, 1819, 2553
BstV2I	GAAGAC	1 cut(s)	680
BstX2I	RGATCY	3 cut(s)	1123, 1170, 2077
BstYI	RGATCY	3 cut(s)	1123, 1170, 2077
BsuI	GTATCC	3 cut(s)	998, 2532, 2560
BsuRI	GGCC	3 cut(s)	242, 1386, 2358
BtgI	CCRYGG	1 cut(s)	265
BtgZI	GCGATG	1 cut(s)	2060
BtrI	CACGTC	1 cut(s)	601
BtsI	GCAGTG	1 cut(s)	1979
BtsIMutI	CAGTG	3 cut(s)	1143, 1688, 1979
BveI	ACCTGC	2 cut(s)	327, 2463
Cac8I	GCNNGC	2 cut(s)	342, 1709
CfoI	GCGC	1 cut(s)	1837
Cfr13I	GGNCC	5 cut(s)	241, 261, 854, 965, 1868
CseI	GACGC	1 cut(s)	2170
Csp6I	GTAC	3 cut(s)	1316, 1531, 2510
CviAII	CATG	5 cut(s)	607, 1213, 1477, 1724, 2069
CviQI	GTAC	3 cut(s)	1316, 1531, 2510
DdeI	CTNAG	4 cut(s)	275, 390, 1418, 2530
DpnI	GATC	7 cut(s)	116, 1125, 1172, 1329, 2079, 2393, 2448
DpnII	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
DraI	TTTAAA	2 cut(s)	786, 1297
Eam1104I	CTCTTC	5 cut(s)	22, 106, 915, 1950, 2160
EarI	CTCTTC	5 cut(s)	22, 106, 915, 1950, 2160
EciI	GGCGGA	1 cut(s)	317
Eco130I	CCWWGG	1 cut(s)	2505
Eco147I	AGGCCT	1 cut(s)	2358
Eco31I	GGTCTC	1 cut(s)	393
Eco32I	GATATC	2 cut(s)	6, 1882
Eco47I	GGWCC	4 cut(s)	261, 854, 965, 1868
Eco57I	CTGAAG	1 cut(s)	1623
EcoRV	GATATC	2 cut(s)	6, 1882
EcoT14I	CCWWGG	1 cut(s)	2505
EcoT22I	ATGCAT	4 cut(s)	622, 1307, 2017, 2070
ErhI	CCWWGG	1 cut(s)	2505
Esp3I	CGTCTC	1 cut(s)	2188
FaeI	CATG	5 cut(s)	610, 1216, 1480, 1727, 2072
FalI	AAGNNNNNCTT	6 cut(s)	1646, 1678, 2157, 2189, 2241, 2273
FaqI	GGGAC	1 cut(s)	247
FatI	CATG	5 cut(s)	606, 1212, 1476, 1723, 2068
FauI	CCCGC	4 cut(s)	300, 362, 1055, 2381
FauNDI	CATATG	1 cut(s)	622
FblI	GTMKAC	2 cut(s)	603, 1146
Fnu4HI	GCNGC	9 cut(s)	56, 64, 323, 408, 1050, 1495, 1705, 1833, 2542
Fsp4HI	GCNGC	9 cut(s)	56, 64, 323, 408, 1050, 1495, 1705, 1833, 2542
FspBI	CTAG	2 cut(s)	567, 992
FspI	TGCGCA	1 cut(s)	1836
GlaI	GCGC	1 cut(s)	1836
GluI	GCNGC	9 cut(s)	56, 64, 323, 408, 1050, 1495, 1705, 1833, 2542
GsuI	CTGGAG	1 cut(s)	9
HaeIII	GGCC	3 cut(s)	242, 1386, 2358
HapII	CCGG	7 cut(s)	334, 347, 545, 1728, 2399, 2482, 2538
HgaI	GACGC	1 cut(s)	2170
HhaI	GCGC	1 cut(s)	1837
Hin1II	CATG	5 cut(s)	610, 1216, 1480, 1727, 2072
Hin6I	GCGC	1 cut(s)	1835
HinP1I	GCGC	1 cut(s)	1835
HincII	GTYRAC	2 cut(s)	604, 2382
HindII	GTYRAC	2 cut(s)	604, 2382
HindIII	AAGCTT	1 cut(s)	932
HinfI	GANTC	8 cut(s)	430, 500, 595, 610, 705, 1731, 2314, 2320
HpaI	GTTAAC	1 cut(s)	2382
HpaII	CCGG	7 cut(s)	334, 347, 545, 1728, 2399, 2482, 2538
HphI	GGTGA	7 cut(s)	308, 833, 883, 1141, 1167, 1421, 1582
Hpy166II	GTNNAC	4 cut(s)	604, 1147, 1531, 2382
Hpy188III	TCNNGA	9 cut(s)	567, 614, 968, 1220, 1336, 1772, 1871, 2081, 2399
Hpy8I	GTNNAC	4 cut(s)	604, 1147, 1531, 2382
Hpy99I	CGWCG	1 cut(s)	605
HpyCH4III	ACNGT	5 cut(s)	437, 838, 850, 1370, 1582
HpyCH4IV	ACGT	5 cut(s)	600, 790, 875, 1110, 2091
HpyF10VI	GCNNNNNNNGC	3 cut(s)	276, 2258, 2331
HpyF3I	CTNAG	4 cut(s)	275, 390, 1418, 2530
HpySE526I	ACGT	5 cut(s)	600, 790, 875, 1110, 2091
Hsp92II	CATG	5 cut(s)	610, 1216, 1480, 1727, 2072
HspAI	GCGC	1 cut(s)	1835
Kpn2I	TCCGGA	1 cut(s)	2398
KspAI	GTTAAC	1 cut(s)	2382
Kzo9I	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
LmnI	GCTCC	4 cut(s)	275, 330, 349, 2428
Lsp1109I	GCAGC	6 cut(s)	309, 394, 1481, 1691, 1819, 2553
MaeI	CTAG	2 cut(s)	567, 992
MaeII	ACGT	5 cut(s)	600, 790, 875, 1110, 2091
MaeIII	GTNAC	8 cut(s)	305, 773, 871, 1190, 1570, 1675, 2095, 2327
MalI	GATC	7 cut(s)	116, 1125, 1172, 1329, 2079, 2393, 2448
MbiI	CCGCTC	2 cut(s)	66, 1852
MboI	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
MflI	RGATCY	3 cut(s)	1123, 1170, 2077
MhlI	GDGCHC	1 cut(s)	2295
MlyI	GAGTC	1 cut(s)	1725
MmeI	TCCRAC	7 cut(s)	153, 252, 477, 793, 1035, 1053, 1238
Mph1103I	ATGCAT	4 cut(s)	622, 1307, 2017, 2070
MroI	TCCGGA	1 cut(s)	2398
MroXI	GAANNNNTTC	1 cut(s)	1658
MslI	CAYNNNNRTG	4 cut(s)	524, 1538, 1722, 1802
MspA1I	CMGCKG	2 cut(s)	1049, 1313
MspI	CCGG	7 cut(s)	334, 347, 545, 1728, 2399, 2482, 2538
MspR9I	CCNGG	2 cut(s)	2483, 2538
Mva1269I	GAATGC	2 cut(s)	576, 1161
MvnI	CGCG	2 cut(s)	55, 2325
MwoI	GCNNNNNNNGC	3 cut(s)	276, 2258, 2331
NciI	CCSGG	2 cut(s)	2483, 2538
NdeI	CATATG	1 cut(s)	622
NdeII	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
NlaIII	CATG	5 cut(s)	610, 1216, 1480, 1727, 2072
NlaIV	GGNNCC	5 cut(s)	62, 263, 271, 2079, 2292
NmuCI	GTSAC	4 cut(s)	305, 871, 1570, 2095
NsbI	TGCGCA	1 cut(s)	1836
NsiI	ATGCAT	4 cut(s)	622, 1307, 2017, 2070
NspI	RCATGY	3 cut(s)	1216, 1480, 1727
NspV	TTCGAA	1 cut(s)	708
PaqCI	CACCTGC	1 cut(s)	327
PceI	AGGCCT	1 cut(s)	2358
PciI	ACATGT	1 cut(s)	1476
PcsI	WCGNNNNNNNCGW	1 cut(s)	881
PctI	GAATGC	2 cut(s)	576, 1161
PdmI	GAANNNNTTC	1 cut(s)	1658
PfeI	GAWTC	7 cut(s)	430, 500, 595, 610, 705, 2314, 2320
PfoI	TCCNGGA	1 cut(s)	2481
PkrI	GCNGC	9 cut(s)	57, 65, 324, 409, 1051, 1496, 1706, 1834, 2543
PleI	GAGTC	1 cut(s)	1725
PpsI	GAGTC	1 cut(s)	1725
Ppu21I	YACGTR	1 cut(s)	1111
PscI	ACATGT	1 cut(s)	1476
PsiI	TTATAA	2 cut(s)	1551, 1788
PspN4I	GGNNCC	5 cut(s)	62, 263, 271, 2079, 2292
PspPI	GGNCC	5 cut(s)	241, 261, 854, 965, 1868
PstI	CTGCAG	1 cut(s)	1499
PsuI	RGATCY	3 cut(s)	1123, 1170, 2077
PvuII	CAGCTG	1 cut(s)	1313
RsaI	GTAC	3 cut(s)	1317, 1532, 2511
RsaNI	GTAC	3 cut(s)	1316, 1531, 2510
RseI	CAYNNNNRTG	4 cut(s)	524, 1538, 1722, 1802
SalI	GTCGAC	1 cut(s)	602
SatI	GCNGC	9 cut(s)	56, 64, 323, 408, 1050, 1495, 1705, 1833, 2542
Sau3AI	GATC	7 cut(s)	114, 1123, 1170, 1327, 2077, 2391, 2446
Sau96I	GGNCC	5 cut(s)	241, 261, 854, 965, 1868
SchI	GAGTC	1 cut(s)	1725
ScrFI	CCNGG	2 cut(s)	2483, 2538
SduI	GDGCHC	1 cut(s)	2295
SfcI	CTRYAG	2 cut(s)	634, 1495
SfuI	TTCGAA	1 cut(s)	708
SinI	GGWCC	4 cut(s)	261, 854, 965, 1868
SmiMI	CAYNNNNRTG	4 cut(s)	524, 1538, 1722, 1802
SseBI	AGGCCT	1 cut(s)	2358
SspI	AATATT	1 cut(s)	1251
SspMI	CTAG	2 cut(s)	567, 992
StuI	AGGCCT	1 cut(s)	2358
StyD4I	CCNGG	2 cut(s)	2481, 2536
StyI	CCWWGG	1 cut(s)	2505
TaaI	ACNGT	5 cut(s)	437, 838, 850, 1370, 1582
TaiI	ACGT	5 cut(s)	603, 793, 878, 1113, 2094
TaqI	TCGA	8 cut(s)	255, 363, 484, 603, 615, 708, 1023, 2058
TaqII	GACCGA	1 cut(s)	953
TatI	WGTACW	1 cut(s)	1530
TauI	GCSGC	3 cut(s)	58, 66, 1052
TfiI	GAWTC	7 cut(s)	430, 500, 595, 610, 705, 2314, 2320
TscAI	CASTG	3 cut(s)	1143, 1695, 1979
TseFI	GTSAC	4 cut(s)	305, 871, 1570, 2095
TseI	GCWGC	6 cut(s)	322, 407, 1494, 1704, 1832, 2541
Tsp45I	GTSAC	4 cut(s)	305, 871, 1570, 2095
TspDTI	ATGAA	6 cut(s)	508, 716, 1362, 1430, 2085, 2255
TspGWI	ACGGA	1 cut(s)	2542
TspRI	CASTG	3 cut(s)	1143, 1695, 1979
VpaK11BI	GGWCC	4 cut(s)	261, 854, 965, 1868
XapI	RAATTY	5 cut(s)	475, 722, 1019, 1292, 1545
XbaI	TCTAGA	1 cut(s)	566
XceI	RCATGY	3 cut(s)	1216, 1480, 1727
XmiI	GTMKAC	2 cut(s)	603, 1146
XmnI	GAANNNNTTC	1 cut(s)	1658
XspI	CTAG	2 cut(s)	567, 992
Zsp2I	ATGCAT	4 cut(s)	622, 1307, 2017, 2070

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.