Rh7CG411300

pentatricopeptide repeat-containing protein At1g31840

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002491
Physical Location & Seq
Forward (+)
52842053 .. 52843120
1068 bp
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UTR
Exon/CDS
Intron
Rh7CG411300.1

Sequence Viewer

Length: 1068 bp
ATGTCAAGGCTTTTGGGCAATGGTGAAGTTGAAATGGGATTGAATTTTCATTCGGCAGTGATGGATAGAGGTTTTGTGCTTGATGTAGTTGCTTGTAACAAGATTTTGAAGCTTCTTTGTAGAGAAAATCAGAAAGGAGCGGAGGATTTCTTTGATGCGTTACTAAGGGTTGGTCCGGAGCCGGATGTGGTGACTTTTAGCACCATGATTAACACGTACTGTAAGGATCGGAGGTTGGAAGAGGCAATTAAGCTTTATGAGGTTATGAAAGAGAAGGGTATCAGTCCGGACTTGGTTGTGTATAGCATTCTTATTGACGGTCTCTTTAAGGCAGGGAAGTTGGAGGAGGGGCATCGACTTTTTTCTGTTGCATTAGATAGTGGGATTAAGTTGGATGTAGTCATCTTCAGTTCTGTTATGGATGCATATGTAAGAACTAGAAATTTGGAAAAGCTAGTGGAGGTGTACAGAAGGATGTATAACGAAGGGATCTCACAGAACACTGTTAGTTACAGTGTTCTTATAAACGGCTTGTGCCAGAATGGAAGGATAATGGAGGCTTGTGGGATATTCGGTCAGATTGTGAAGCATGGTTTTGTGCCGTCCATTCTAACTTATAGTAGTTTGATTGATGGAATATGCAAACTGGGGAATTTAAAAGATGCATTTAACCTGTATGAGAGTATGGTCAAGACTGGTTATGAACCTGATATTATTCTTTATGGTGTGCTATTAAATGGCCTTTGCGAGCATGGACTGATGAGTAACGCTCTTAGGTTCATCTTTCAGGTTATTATTAATAGGGGTGTAAAGCCAAATGTTTATACTTTTAATATGTTGATAGATGGCTTCTGTAGATTGAATAGATTAAAAAATGCTGTGAAGGTGTACAACCAAATGCGAATGTATAACATAAGACCAGATGTGGTGACGTATACTGTGCTTATTCGAGGCATTTCTGAATTAGGGAGATTAAATGATGCATTAGTGTTTTTCTTCCAGTCATTGAAGAGGGGTTTCTTGCCCGATGTTGTAACATATTGCACCCTCATTGATGCTTGCTGCTAG

Protein Analysis

355

Amino Acids

40.09

Weight (kDa)

7.93

Isoelectric Point (pI)

22.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 28 - 75 7.1e-10 PPR repeat family
TPR_24 PF23276 47 - 158 1.1e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 56 - 106 1.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 58 - 90 9.1e-12 PPR repeat
PPR_2 PF13041 61 - 110 1.2e-18 PPR repeat family
PPR PF01535 64 - 94 5.3e-09 PPR repeat
PPR_1 PF12854 92 - 122 5e-08 PPR repeat
PPR_3 PF13812 126 - 176 1.9e-08 Pentatricopeptide repeat domain
TPR_24 PF23276 126 - 229 4.7e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 132 - 180 4.2e-11 PPR repeat family
PPR_1 PF12854 163 - 193 4.4e-08 PPR repeat
PPR_2 PF13041 167 - 215 1.6e-13 PPR repeat family
PPR PF01535 169 - 199 1e-06 PPR repeat
PPR_3 PF13812 190 - 246 1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 198 - 230 2e-09 PPR repeat
PPR PF01535 205 - 233 5.5e-06 PPR repeat
PPR_2 PF13041 208 - 249 1.6e-10 PPR repeat family
PPR_2 PF13041 236 - 286 7.4e-12 PPR repeat family
TPR_24 PF23276 261 - 322 2e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 261 - 317 4.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 268 - 301 1.2e-13 PPR repeat
PPR_2 PF13041 272 - 319 3.2e-17 PPR repeat family
PPR PF01535 275 - 304 4.6e-06 PPR repeat
PPR_2 PF13041 315 - 355 4.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 935
AccIII TCCGGA 2 cut(s) 175, 286
AciI CCGC 1 cut(s) 140
AclWI GGATC 2 cut(s) 234, 497
AcsI RAATTY 3 cut(s) 43, 442, 652
AcuI CTGAAG 1 cut(s) 391
AfaI GTAC 3 cut(s) 218, 467, 890
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 5 cut(s) 32, 43, 109, 862, 1009
AluBI AGCT 3 cut(s) 112, 253, 454
AluI AGCT 3 cut(s) 112, 253, 454
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 2 cut(s) 234, 497
Aor13HI TCCGGA 2 cut(s) 175, 286
AoxI GGCC 1 cut(s) 739
ApeKI GCWGC 1 cut(s) 1062
ApoI RAATTY 3 cut(s) 43, 442, 652
AseI ATTAAT 1 cut(s) 798
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 3 cut(s) 35, 202, 940
AvaII GGWCC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 1049
BccI CCATC 3 cut(s) 55, 626, 839
BceAI ACGGC 2 cut(s) 544, 586
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 3 cut(s) 438, 455, 1066
BfmI CTRYAG 1 cut(s) 853
BisI GCNGC 1 cut(s) 1063
BlsI GCNGC 1 cut(s) 1064
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 180
BmrI ACTGGG 1 cut(s) 656
BmsI GCATC 6 cut(s) 145, 361, 412, 652, 970, 1045
BmuI ACTGGG 1 cut(s) 656
BplI GAGNNNNNCTC 2 cut(s) 754, 786
BsaAI YACGTR 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 326
BsaWI WCCGGW 2 cut(s) 175, 286
Bse1I ACTGG 3 cut(s) 651, 700, 1000
Bse3DI GCAATG 1 cut(s) 25
BseAI TCCGGA 2 cut(s) 175, 286
BseGI GGATG 4 cut(s) 190, 400, 427, 480
BseMI GCAATG 1 cut(s) 25
BseNI ACTGG 3 cut(s) 651, 700, 1000
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 1 cut(s) 1049
BshFI GGCC 1 cut(s) 741
BsiSI CCGG 3 cut(s) 176, 182, 287
BsmAI GTCTC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 1 cut(s) 741
Bso31I GGTCTC 1 cut(s) 326
Bsp13I TCCGGA 2 cut(s) 175, 286
Bsp1407I TGTACA 2 cut(s) 465, 888
Bsp143I GATC 2 cut(s) 226, 489
BspACI CCGC 1 cut(s) 140
BspANI GGCC 1 cut(s) 741
BspEI TCCGGA 2 cut(s) 175, 286
BspLI GGNNCC 1 cut(s) 180
BspPI GGATC 2 cut(s) 234, 497
BspTNI GGTCTC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 25
BsrGI TGTACA 2 cut(s) 465, 888
BsrI ACTGG 3 cut(s) 651, 700, 1000
BssMI GATC 2 cut(s) 226, 489
BssNAI GTATAC 1 cut(s) 936
Bst1107I GTATAC 1 cut(s) 936
Bst4CI ACNGT 5 cut(s) 221, 320, 505, 515, 940
Bst6I CTCTTC 2 cut(s) 234, 1004
BstAUI TGTACA 2 cut(s) 465, 888
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 749, 1060
BstDEI CTNAG 2 cut(s) 164, 773
BstF5I GGATG 4 cut(s) 190, 400, 427, 480
BstKTI GATC 2 cut(s) 229, 492
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 2 cut(s) 226, 489
BstSFI CTRYAG 1 cut(s) 853
BstV1I GCAGC 1 cut(s) 1049
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
BstZ17I GTATAC 1 cut(s) 936
BsuRI GGCC 1 cut(s) 741
BtsCI GGATG 4 cut(s) 190, 400, 427, 480
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 63, 501, 520
Cac8I GCNNGC 2 cut(s) 749, 1060
Cfr13I GGNCC 1 cut(s) 173
Csp6I GTAC 3 cut(s) 217, 466, 889
CviAII CATG 3 cut(s) 205, 590, 752
CviQI GTAC 3 cut(s) 217, 466, 889
DdeI CTNAG 2 cut(s) 164, 773
DpnI GATC 2 cut(s) 228, 491
DpnII GATC 2 cut(s) 226, 489
DraI TTTAAA 1 cut(s) 657
Eam1104I CTCTTC 2 cut(s) 234, 1004
EarI CTCTTC 2 cut(s) 234, 1004
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 391
EcoT22I ATGCAT 3 cut(s) 427, 667, 985
FaeI CATG 3 cut(s) 208, 593, 755
FatI CATG 3 cut(s) 204, 589, 751
FauNDI CATATG 1 cut(s) 427
FblI GTMKAC 1 cut(s) 935
Fnu4HI GCNGC 1 cut(s) 1063
FokI GGATG 4 cut(s) 197, 407, 434, 487
Fsp4HI GCNGC 1 cut(s) 1063
FspBI CTAG 3 cut(s) 438, 455, 1066
GluI GCNGC 1 cut(s) 1063
HaeIII GGCC 1 cut(s) 741
HapII CCGG 3 cut(s) 176, 182, 287
Hin1II CATG 3 cut(s) 208, 593, 755
HindIII AAGCTT 2 cut(s) 110, 251
HpaII CCGG 3 cut(s) 176, 182, 287
HphI GGTGA 3 cut(s) 35, 202, 940
Hpy166II GTNNAC 3 cut(s) 466, 889, 936
Hpy188I TCNGA 4 cut(s) 132, 231, 579, 961
Hpy188III TCNNGA 3 cut(s) 176, 287, 691
Hpy8I GTNNAC 3 cut(s) 466, 889, 936
HpyAV CCTTC 5 cut(s) 268, 465, 479, 540, 877
HpyCH4III ACNGT 5 cut(s) 221, 320, 505, 515, 940
HpyCH4IV ACGT 2 cut(s) 215, 932
HpyCH4V TGCA 6 cut(s) 371, 425, 642, 665, 983, 1044
HpyF3I CTNAG 2 cut(s) 164, 773
HpySE526I ACGT 2 cut(s) 215, 932
Hsp92II CATG 3 cut(s) 208, 593, 755
Kpn2I TCCGGA 2 cut(s) 175, 286
Kzo9I GATC 2 cut(s) 226, 489
LmnI GCTCC 2 cut(s) 137, 178
Lsp1109I GCAGC 1 cut(s) 1049
LweI GCATC 6 cut(s) 145, 361, 412, 652, 970, 1045
MaeI CTAG 3 cut(s) 438, 455, 1066
MaeII ACGT 2 cut(s) 215, 932
MaeIII GTNAC 7 cut(s) 95, 159, 190, 509, 764, 928, 1033
MalI GATC 2 cut(s) 228, 491
MbiI CCGCTC 1 cut(s) 140
MboI GATC 2 cut(s) 226, 489
MboII GAAGA 4 cut(s) 251, 397, 988, 1021
MflI RGATCY 1 cut(s) 489
MluCI AATT 5 cut(s) 43, 246, 442, 652, 962
MmeI TCCRAC 3 cut(s) 216, 321, 372
Mph1103I ATGCAT 3 cut(s) 427, 667, 985
MroI TCCGGA 2 cut(s) 175, 286
MspI CCGG 3 cut(s) 176, 182, 287
Mva1269I GAATGC 1 cut(s) 306
NdeI CATATG 1 cut(s) 427
NdeII GATC 2 cut(s) 226, 489
NlaIII CATG 3 cut(s) 208, 593, 755
NlaIV GGNNCC 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 190, 928
NsiI ATGCAT 3 cut(s) 427, 667, 985
PctI GAATGC 1 cut(s) 306
PkrI GCNGC 1 cut(s) 1064
Ppu21I YACGTR 1 cut(s) 216
PshBI ATTAAT 1 cut(s) 798
PsiI TTATAA 1 cut(s) 524
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 173
PsuI RGATCY 1 cut(s) 489
RsaI GTAC 3 cut(s) 218, 467, 890
RsaNI GTAC 3 cut(s) 217, 466, 889
SatI GCNGC 1 cut(s) 1063
Sau3AI GATC 2 cut(s) 226, 489
Sau96I GGNCC 1 cut(s) 173
SfaNI GCATC 6 cut(s) 145, 361, 412, 652, 970, 1045
SfcI CTRYAG 1 cut(s) 853
SinI GGWCC 1 cut(s) 173
Sse9I AATT 5 cut(s) 43, 246, 442, 652, 962
SsiI CCGC 1 cut(s) 140
SspMI CTAG 3 cut(s) 438, 455, 1066
TaaI ACNGT 5 cut(s) 221, 320, 505, 515, 940
TaiI ACGT 2 cut(s) 218, 935
TaqI TCGA 2 cut(s) 355, 949
TaqII GACCGA 1 cut(s) 563
TasI AATT 5 cut(s) 43, 246, 442, 652, 962
TatI WGTACW 2 cut(s) 465, 888
TscAI CASTG 3 cut(s) 63, 508, 520
TseFI GTSAC 2 cut(s) 190, 928
TseI GCWGC 1 cut(s) 1062
Tsp45I GTSAC 2 cut(s) 190, 928
TspDTI ATGAA 4 cut(s) 38, 281, 717, 769
TspRI CASTG 3 cut(s) 63, 508, 520
VpaK11BI GGWCC 1 cut(s) 173
VspI ATTAAT 1 cut(s) 798
XapI RAATTY 3 cut(s) 43, 442, 652
XmiI GTMKAC 1 cut(s) 935
XspI CTAG 3 cut(s) 438, 455, 1066
Zsp2I ATGCAT 3 cut(s) 427, 667, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.