Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002491

Physical Location & Seq

Genomic Coordinates Reverse (-)

18898464 .. 18900968

Gene Structure 2505 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001660

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGATATCTTCTTCTTCTTCTTCCACCTCTTCATTCTCTACCCTAGTCCATCTCACGCGCCGCCGCTCTCTTCTTCTCTACTCCACCTCCCCATTCTCCTCCAAAACCCTAATCCACCAAATCTCAACCGCCTTCCTCCAAAACAACCCCAAGCTCTTACCCCCCGTTTCCGTCTCCAAATTACACCCACACCACGCCCCCCCCATCCTCCTCCGCCTCCAGTCCGACCCCGTCGCCGCCCTCCGCTTCTTCGACTGGTCCCGCACCTCTCTCGGCTTCACCCACTCGCCGCACTCCTTCTCCGCCCTCATTCACCTCCTGCTCCGCCGCCGATTGCTCGCTCCGGCTTCTCGGGTTTTCGACACCATGGTACGCCAATTCGGTACTCAATTTAACATTTCTGCTGTTTTTACAGAGAATTTTAGTAGCTACGGTTCAAATTTGAGTTATGTTGGTGGTTTTCTGATTGAGAATTTTTGCCGAAATGGGGAGTTGGATTCTGCTGTTGAGGCATTGGTTTGTATGTATACATTGGGTGCTTCGGTGCCGCCTTTTGCTGTGTGTAGAATTTTGAGTGCTTGTGTTGATACGAATCGTGTACATGTGATTCTTGATATATATGGAAAATTGTGTGGACAAGGTTTTGGTGTTTACGAGTTTGTTATGTCAAGGCTTTTGGGCAAAGGTGAAGTTGAAATGGGATTGAATTTTCATTCGACAGTGATGGATAGAGGCTTTGTGCTTGATGTAGTTGCTTGTAACAAGATTTTGAAGCTTCTTTGTAGAGAAAATCAGAAAGGAGGGGAGGATTTCTTTGATGTGTTACTAAGGGTTGGTCCGGAGCCGAATGTGGTGACTTTTAGCACCATGATTAACACGTACTGTAAGGATCGGAGGTTGGAAGAGGCAATTAAGCTTTATGAGGTTATGAAAGAGAAGGGTATCAGTCCGGACTTGGTTGTGTATAGTATTCTTATTGACGGTCTCTTTAAGGCAGGGAAGTTGGAGGAGGGGCATCGACTTTTTTCAGTTGCATTAGATAGTGGGATTAAGTTGGATGTAGTCATCTTGAGTTCTGTTATGGATGCATATGTAAGAACTAGAAATTTGGAAAAGCTAGTGGAGGTGTACAGAAGGATGTATAACGAAGGGATCTCACAGAACACTGTTAGTTACAGTGTTCTTATAAACGGCTTGTGCCAGAATGGAAGGATAATGGAGGCTTGTGGGATATTCGGTCAGATTGTGAAGCATGGTTTTGTGCCGTCCATTCTAACTTATAGTAGTTTGATTGATGGAATATGCAAACTGGGGAATTTAAAAGATGCATTTAACCTGTATGAGAGTATGGTCAAGACTGGTTATGAACCTGATATTATTCTTTATGGTGTGCTATTAAATGGCCTTTGCAAGCATGGACTGATGAGTGACGCTCTTAGGTTCTTCTTTCAGGTTATTAATAGAGGTGTAAAGCCAAATGTTTATACTCTTAATATGTTGATAGATGGCTTCTGTAGATTGAATAGATTAAAAATTGCTGTGAAGGTGTACAACCAAATGCGAATGTATAACATAAGACCAGATGTGGTGACGTATACTGTGCTTATTCGAGGCATTTCTGAATTGGGGAGACTAAATGATGCATTAGTGTTTTTCTTCCAATCATTGAAGAGGGGTTTCTTGCCTGATGTTGTAACATATTGCACCCTCATTGATGCTTGCTGCAAGCAGAAATATGTATATGCTGGCTTCCGGATTTTTGAGATGATGCTAATAAATCGAGTCAATCCTGATATTACCATTTATAATGTTCTCATTAATATGCTTTTCAAAGAAGGTTATTTAGAAGCTGCACAGACACTCTTTGAACAACTTACTGAAAGAGGGCTACGACCTGATATGGTTACATACAATACAATGGTTTGTGGTTATTGTTCTCTGAGAAGGTTAGATGAAGCTGTTCGCCTTTTTCAACAAATGACACAGGGACAGTGTAAACCCAATGCCATCACTTGTAGTATATTGATCGATGCTTTCTGTAAAGAAGGTAAAATGGACGATGCAGTGTTATTGTTTGATCAAATGCTGGAAAAGGATCCTGAACCTAATGTGGTCACCTATAGTTGTCTGATTGATGGTTATTTTAAATCTGAAAACATGGAAAGTGCCTTTGAACTATATGAAGAGATGCTTCAGAGTATCTCTCCAAATATAGTTAGTTACAGCATCCTCATTGATGGCCTTTGCAAAAGAGGACTGATGAAGAAAGCAGCCGTTGCATTTCATTCTGCTATAGACAGGGGTCTGGTGCCGGATGTAGTAGCATATGGGATTCTGATTCATGGTTACTGCAAGGTTGGAAGAACAGCAGAAGCCATGGTATTGTACGGTCGTATGTTAATAAGTGGAATCATGCCAGATGCTATCATACAAAGGATGATTGCGAAGCACCTTCTTGAAATTCGGTCCCACCAAAATATGAGTGCCCAGCCAGATGTTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

835

Amino Acids

93.84

Weight (kDa)

8.44

Isoelectric Point (pI)

35.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	249 - 292	6.9e-06	PPR repeat family
TPR_24	PF23276	272 - 379	3.8e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	279 - 311	3.1e-11	PPR repeat
PPR_3	PF13812	279 - 326	4.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	282 - 331	3.7e-18	PPR repeat family
PPR	PF01535	285 - 315	1.5e-08	PPR repeat
PPR_1	PF12854	313 - 343	1.4e-07	PPR repeat
PPR_3	PF13812	347 - 397	7.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	353 - 401	1.2e-09	PPR repeat family
PPR_1	PF12854	384 - 414	1.3e-07	PPR repeat
PPR_2	PF13041	388 - 436	4.9e-13	PPR repeat family
PPR	PF01535	390 - 420	3e-06	PPR repeat
PPR_3	PF13812	411 - 467	3.3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	419 - 451	5.7e-09	PPR repeat
PPR_2	PF13041	422 - 471	2.5e-13	PPR repeat family
PPR_1	PF12854	454 - 482	1.6e-06	PPR repeat
PPR_2	PF13041	457 - 506	1.9e-13	PPR repeat family
PPR_3	PF13812	486 - 537	9.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	488 - 521	3e-11	PPR repeat
PPR_2	PF13041	496 - 539	2.5e-13	PPR repeat family
PPR_2	PF13041	528 - 576	5e-12	PPR repeat family
PPR_3	PF13812	558 - 609	1.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	558 - 590	2.4e-06	PPR repeat
PPR_2	PF13041	562 - 599	2.4e-06	PPR repeat family
PPR_long	PF17177	575 - 685	1.4e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	597 - 645	4.2e-14	PPR repeat family
TPR_24	PF23276	603 - 730	4.6e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	621 - 681	3.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	628 - 661	1.4e-12	PPR repeat
PPR_2	PF13041	632 - 681	1.2e-17	PPR repeat family
PPR	PF01535	635 - 662	1.4e-07	PPR repeat
PPR_1	PF12854	665 - 695	7.2e-11	PPR repeat
PPR	PF01535	670 - 698	3.9e-07	PPR repeat
PPR_2	PF13041	671 - 716	5.6e-14	PPR repeat family
PPR_3	PF13812	691 - 746	6.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	701 - 730	1.1e-09	PPR repeat
PPR_2	PF13041	702 - 750	1.4e-17	PPR repeat family
PPR	PF01535	705 - 732	6.8e-07	PPR repeat
PPR_1	PF12854	734 - 759	5e-08	PPR repeat
PPR_1	PF12854	767 - 799	4.4e-10	PPR repeat
PPR_2	PF13041	771 - 808	2.4e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31840 AT1G31840
malus_domestica	MD12G1033400.v1.1
prunus_persica	Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1
pyrus_communis	pycom12g03070
rosa_chinensis	RchiOBHm_Chr2g0139321 RchiOBHm_Chr7g0227311
rosa_laevigata	RLG00000001660
rosa_multiflora	Rmu_sc0001246.1_g000012 Rmu_sc0002525.1_g000013 Rmu_sc0003153.1_g000006 Rmu_sc0011922.1_g000005
rosa_roxburghii	Rroxscaffold_3G00232240
rosa_rugosa	Rorug07G0241200
rosa_samantha	Rh2BG417100 Rh7AG391100 Rh7AG391600 Rh7AG392200 Rh7AG392500 Rh7BG376100 Rh7CG192200 Rh7CG410100 Rh7CG410500 Rh7CG411300 Rh7CG411800 Rh7DG388700
rosa_wichuraiana	Rw7G032710 Rw7G032740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1187, 1806
AccB1I	GGYRCC	2 cut(s)	544, 2308
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	2 cut(s)	527, 1595
AccII	CGCG	1 cut(s)	58
AccIII	TCCGGA	3 cut(s)	838, 949, 1752
AclWI	GGATC	4 cut(s)	897, 1160, 2090, 2103
AcsI	RAATTY	8 cut(s)	418, 439, 472, 567, 706, 1105, 1315, 2460
AcuI	CTGAAG	1 cut(s)	2177
AfaI	GTAC	7 cut(s)	372, 385, 600, 881, 1130, 1550, 2387
AfiI	CCNNNNNNNGG	1 cut(s)	487
AflIII	ACRYGT	2 cut(s)	601, 876
AluBI	AGCT	7 cut(s)	154, 429, 775, 916, 1117, 1850, 1958
AluI	AGCT	7 cut(s)	154, 429, 775, 916, 1117, 1850, 1958
Alw26I	GTCTC	3 cut(s)	178, 989, 1624
AlwI	GGATC	4 cut(s)	897, 1160, 2090, 2103
Ama87I	CYCGRG	1 cut(s)	351
Aor13HI	TCCGGA	3 cut(s)	838, 949, 1752
AoxI	GGCC	2 cut(s)	1402, 2239
ApeKI	GCWGC	3 cut(s)	1722, 1850, 2270
ApoI	RAATTY	8 cut(s)	418, 439, 472, 567, 706, 1105, 1315, 2460
AseI	ATTAAT	2 cut(s)	1458, 1818
Asp700I	GAANNNNTTC	1 cut(s)	1959
AspLEI	GCGC	1 cut(s)	60
AspS9I	GGNCC	3 cut(s)	258, 836, 2466
AsuHPI	GGTGA	6 cut(s)	271, 305, 698, 865, 1600, 2107
AvaI	CYCGRG	1 cut(s)	351
AvaII	GGWCC	3 cut(s)	258, 836, 2466
BaeGI	GKGCMC	1 cut(s)	2488
BamHI	GGATCC	1 cut(s)	2095
BanI	GGYRCC	2 cut(s)	544, 2308
BbvI	GCAGC	3 cut(s)	1709, 1837, 2282
BccI	CCATC	8 cut(s)	57, 212, 718, 1289, 1499, 2015, 2129, 2231
BceAI	ACGGC	3 cut(s)	1207, 1249, 2258
BcgI	CGANNNNNNTGC	2 cut(s)	253, 287
BclI	TGATCA	1 cut(s)	2077
BcoDI	GTCTC	3 cut(s)	178, 989, 1624
BfaI	CTAG	3 cut(s)	44, 1101, 1118
BfmI	CTRYAG	3 cut(s)	1513, 2119, 2292
BisI	GCNGC	9 cut(s)	61, 64, 237, 290, 328, 548, 1723, 1851, 2271
BlsI	GCNGC	9 cut(s)	62, 65, 238, 291, 329, 549, 1724, 1852, 2272
Bme18I	GGWCC	3 cut(s)	258, 836, 2466
BmeT110I	CYCGRG	1 cut(s)	351
BmgT120I	GGNCC	3 cut(s)	258, 836, 2466
BmiI	GGNNCC	6 cut(s)	260, 546, 843, 2097, 2310, 2468
BmrI	ACTGGG	1 cut(s)	1319
BmuI	ACTGGG	1 cut(s)	1319
BoxI	GACNNNNGTC	1 cut(s)	2301
BplI	GAGNNNNNCTC	4 cut(s)	1417, 1449, 2188, 2220
BpmI	CTGGAG	1 cut(s)	203
BpuEI	CTTGAG	1 cut(s)	1090
Bsa29I	ATCGAT	1 cut(s)	2028
BsaAI	YACGTR	1 cut(s)	879
BsaBI	GATNNNNATC	1 cut(s)	591
BsaI	GGTCTC	1 cut(s)	989
BsaJI	CCNNGG	2 cut(s)	366, 2376
BsaWI	WCCGGW	3 cut(s)	838, 949, 1752
BsaXI	ACNNNNNCTCC	4 cut(s)	71, 101, 284, 314
Bsc4I	CCNNNNNNNGG	1 cut(s)	487
Bse1I	ACTGG	4 cut(s)	220, 260, 1314, 1363
Bse8I	GATNNNNATC	1 cut(s)	591
BseAI	TCCGGA	3 cut(s)	838, 949, 1752
BseCI	ATCGAT	1 cut(s)	2028
BseDI	CCNNGG	2 cut(s)	366, 2376
BseGI	GGATG	7 cut(s)	204, 1063, 1090, 1143, 2226, 2320, 2442
BseJI	GATNNNNATC	1 cut(s)	591
BseLI	CCNNNNNNNGG	1 cut(s)	487
BseMII	CTCAG	1 cut(s)	1931
BseNI	ACTGG	4 cut(s)	220, 260, 1314, 1363
BseRI	GAGGAG	3 cut(s)	88, 200, 1022
BseSI	GKGCMC	1 cut(s)	2488
BseXI	GCAGC	3 cut(s)	1709, 1837, 2282
BseYI	CCCAGC	1 cut(s)	2487
BsgI	GTGCAG	1 cut(s)	1836
Bsh1236I	CGCG	1 cut(s)	58
Bsh1285I	CGRYCG	1 cut(s)	2392
BshFI	GGCC	2 cut(s)	1404, 2241
BshNI	GGYRCC	2 cut(s)	544, 2308
BshVI	ATCGAT	1 cut(s)	2028
BsiEI	CGRYCG	1 cut(s)	2392
BsiHKCI	CYCGRG	1 cut(s)	351
BsiSI	CCGG	5 cut(s)	344, 839, 950, 1753, 2312
BslFI	GGGAC	3 cut(s)	244, 2001, 2452
BslI	CCNNNNNNNGG	1 cut(s)	487
BsmAI	GTCTC	3 cut(s)	178, 989, 1624
BsmBI	CGTCTC	1 cut(s)	178
BsmFI	GGGAC	3 cut(s)	244, 2001, 2452
BsnI	GGCC	2 cut(s)	1404, 2241
Bso31I	GGTCTC	1 cut(s)	989
BsoBI	CYCGRG	1 cut(s)	351
Bsp1286I	GDGCHC	1 cut(s)	2488
Bsp13I	TCCGGA	3 cut(s)	838, 949, 1752
Bsp1407I	TGTACA	3 cut(s)	598, 1128, 1548
Bsp143I	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
Bsp19I	CCATGG	2 cut(s)	366, 2376
BspANI	GGCC	2 cut(s)	1404, 2241
BspCNI	CTCAG	1 cut(s)	1932
BspDI	ATCGAT	1 cut(s)	2028
BspEI	TCCGGA	3 cut(s)	838, 949, 1752
BspFNI	CGCG	1 cut(s)	58
BspLI	GGNNCC	6 cut(s)	260, 546, 843, 2097, 2310, 2468
BspPI	GGATC	4 cut(s)	897, 1160, 2090, 2103
BspT107I	GGYRCC	2 cut(s)	544, 2308
BspTNI	GGTCTC	1 cut(s)	989
BsrBI	CCGCTC	1 cut(s)	66
BsrGI	TGTACA	3 cut(s)	598, 1128, 1548
BsrI	ACTGG	4 cut(s)	220, 260, 1314, 1363
BssECI	CCNNGG	2 cut(s)	366, 2376
BssMI	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
BssNAI	GTATAC	2 cut(s)	528, 1596
BssT1I	CCWWGG	2 cut(s)	366, 2376
Bst1107I	GTATAC	2 cut(s)	528, 1596
Bst4CI	ACNGT	9 cut(s)	434, 721, 884, 983, 1168, 1178, 1600, 1992, 2390
Bst6I	CTCTTC	5 cut(s)	34, 75, 897, 1664, 2178
BstAPI	GCANNNNNTGC	1 cut(s)	2276
BstAUI	TGTACA	3 cut(s)	598, 1128, 1548
BstBAI	YACGTR	1 cut(s)	879
BstC8I	GCNNGC	5 cut(s)	339, 1412, 1720, 1727, 1747
BstDEI	CTNAG	3 cut(s)	827, 1436, 1940
BstDSI	CCRYGG	2 cut(s)	366, 2376
BstEII	GGTNACC	1 cut(s)	2113
BstF5I	GGATG	7 cut(s)	204, 1063, 1090, 1143, 2226, 2320, 2442
BstFNI	CGCG	1 cut(s)	58
BstHHI	GCGC	1 cut(s)	60
BstKTI	GATC	5 cut(s)	892, 1155, 2028, 2080, 2098
BstMAI	GTCTC	3 cut(s)	178, 989, 1624
BstMBI	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
BstMCI	CGRYCG	1 cut(s)	2392
BstMWI	GCNNNNNNNGC	2 cut(s)	509, 2276
BstNSI	RCATGY	1 cut(s)	605
BstPAI	GACNNNNGTC	1 cut(s)	2301
BstPI	GGTNACC	1 cut(s)	2113
BstSFI	CTRYAG	3 cut(s)	1513, 2119, 2292
BstSLI	GKGCMC	1 cut(s)	2488
BstUI	CGCG	1 cut(s)	58
BstV1I	GCAGC	3 cut(s)	1709, 1837, 2282
BstX2I	RGATCY	2 cut(s)	1152, 2095
BstYI	RGATCY	2 cut(s)	1152, 2095
BstZ17I	GTATAC	2 cut(s)	528, 1596
Bsu15I	ATCGAT	1 cut(s)	2028
BsuRI	GGCC	2 cut(s)	1404, 2241
BsuTUI	ATCGAT	1 cut(s)	2028
BtgI	CCRYGG	2 cut(s)	366, 2376
BtsCI	GGATG	7 cut(s)	204, 1063, 1090, 1143, 2226, 2320, 2442
BtsI	GCAGTG	1 cut(s)	2070
BtsIMutI	CAGTG	5 cut(s)	726, 1164, 1183, 1997, 2070
Cac8I	GCNNGC	5 cut(s)	339, 1412, 1720, 1727, 1747
CfoI	GCGC	1 cut(s)	60
Cfr13I	GGNCC	3 cut(s)	258, 836, 2466
ClaI	ATCGAT	1 cut(s)	2028
CseI	GACGC	1 cut(s)	1439
Csp6I	GTAC	7 cut(s)	371, 384, 599, 880, 1129, 1549, 2386
CviAII	CATG	9 cut(s)	367, 602, 868, 1253, 1415, 2158, 2342, 2377, 2413
CviQI	GTAC	7 cut(s)	371, 384, 599, 880, 1129, 1549, 2386
DdeI	CTNAG	3 cut(s)	827, 1436, 1940
DpnI	GATC	5 cut(s)	891, 1154, 2027, 2079, 2097
DpnII	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
DraI	TTTAAA	2 cut(s)	1320, 2146
Eam1104I	CTCTTC	5 cut(s)	34, 75, 897, 1664, 2178
EarI	CTCTTC	5 cut(s)	34, 75, 897, 1664, 2178
EciI	GGCGGA	3 cut(s)	203, 292, 314
Eco130I	CCWWGG	2 cut(s)	366, 2376
Eco31I	GGTCTC	1 cut(s)	989
Eco32I	GATATC	1 cut(s)	6
Eco47I	GGWCC	3 cut(s)	258, 836, 2466
Eco57I	CTGAAG	1 cut(s)	2177
Eco88I	CYCGRG	1 cut(s)	351
Eco91I	GGTNACC	1 cut(s)	2113
EcoO65I	GGTNACC	1 cut(s)	2113
EcoRV	GATATC	1 cut(s)	6
EcoT14I	CCWWGG	2 cut(s)	366, 2376
EcoT22I	ATGCAT	3 cut(s)	1090, 1330, 1645
ErhI	CCWWGG	2 cut(s)	366, 2376
Esp3I	CGTCTC	1 cut(s)	178
FaeI	CATG	9 cut(s)	370, 605, 871, 1256, 1418, 2161, 2345, 2380, 2416
FalI	AAGNNNNNCTT	2 cut(s)	2175, 2207
FaqI	GGGAC	3 cut(s)	244, 2001, 2452
FatI	CATG	9 cut(s)	366, 601, 867, 1252, 1414, 2157, 2341, 2376, 2412
FauI	CCCGC	1 cut(s)	269
FauNDI	CATATG	2 cut(s)	1090, 2326
FbaI	TGATCA	1 cut(s)	2077
FblI	GTMKAC	2 cut(s)	527, 1595
Fnu4HI	GCNGC	9 cut(s)	61, 64, 237, 290, 328, 548, 1723, 1851, 2271
FokI	GGATG	7 cut(s)	191, 1070, 1097, 1150, 2213, 2327, 2449
Fsp4HI	GCNGC	9 cut(s)	61, 64, 237, 290, 328, 548, 1723, 1851, 2271
FspBI	CTAG	3 cut(s)	44, 1101, 1118
GlaI	GCGC	1 cut(s)	59
GluI	GCNGC	9 cut(s)	61, 64, 237, 290, 328, 548, 1723, 1851, 2271
GsaI	CCCAGC	1 cut(s)	2491
GsuI	CTGGAG	1 cut(s)	203
HaeIII	GGCC	2 cut(s)	1404, 2241
HapII	CCGG	5 cut(s)	344, 839, 950, 1753, 2312
HgaI	GACGC	1 cut(s)	1439
HhaI	GCGC	1 cut(s)	60
Hin1II	CATG	9 cut(s)	370, 605, 871, 1256, 1418, 2161, 2345, 2380, 2416
Hin6I	GCGC	1 cut(s)	58
HinP1I	GCGC	1 cut(s)	58
HindIII	AAGCTT	2 cut(s)	773, 914
HinfI	GANTC	7 cut(s)	497, 592, 607, 1782, 2332, 2338, 2409
HpaII	CCGG	5 cut(s)	344, 839, 950, 1753, 2312
HphI	GGTGA	6 cut(s)	271, 305, 698, 865, 1600, 2107
Hpy166II	GTNNAC	8 cut(s)	528, 599, 635, 652, 1129, 1549, 1596, 1997
Hpy188III	TCNNGA	9 cut(s)	611, 839, 950, 1069, 1354, 1753, 1790, 2099, 2456
Hpy8I	GTNNAC	8 cut(s)	528, 599, 635, 652, 1129, 1549, 1596, 1997
Hpy99I	CGWCG	1 cut(s)	236
HpyCH4III	ACNGT	9 cut(s)	434, 721, 884, 983, 1168, 1178, 1600, 1992, 2390
HpyCH4IV	ACGT	2 cut(s)	878, 1592
HpyF10VI	GCNNNNNNNGC	2 cut(s)	509, 2276
HpyF3I	CTNAG	3 cut(s)	827, 1436, 1940
HpySE526I	ACGT	2 cut(s)	878, 1592
Hsp92II	CATG	9 cut(s)	370, 605, 871, 1256, 1418, 2161, 2345, 2380, 2416
HspAI	GCGC	1 cut(s)	58
Kpn2I	TCCGGA	3 cut(s)	838, 949, 1752
Ksp22I	TGATCA	1 cut(s)	2077
Kzo9I	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
LmnI	GCTCC	3 cut(s)	327, 346, 841
Lsp1109I	GCAGC	3 cut(s)	1709, 1837, 2282
MaeI	CTAG	3 cut(s)	44, 1101, 1118
MaeII	ACGT	2 cut(s)	878, 1592
MalI	GATC	5 cut(s)	891, 1154, 2027, 2079, 2097
MbiI	CCGCTC	1 cut(s)	66
MboI	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
MflI	RGATCY	2 cut(s)	1152, 2095
MhlI	GDGCHC	1 cut(s)	2488
MlyI	GAGTC	1 cut(s)	1791
MmeI	TCCRAC	6 cut(s)	249, 474, 879, 984, 1035, 2338
Mph1103I	ATGCAT	3 cut(s)	1090, 1330, 1645
MroI	TCCGGA	3 cut(s)	838, 949, 1752
MroXI	GAANNNNTTC	1 cut(s)	1959
MslI	CAYNNNNRTG	1 cut(s)	1820
MspI	CCGG	5 cut(s)	344, 839, 950, 1753, 2312
MvnI	CGCG	1 cut(s)	58
MwoI	GCNNNNNNNGC	2 cut(s)	509, 2276
NcoI	CCATGG	2 cut(s)	366, 2376
NdeI	CATATG	2 cut(s)	1090, 2326
NdeII	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
NlaIII	CATG	9 cut(s)	370, 605, 871, 1256, 1418, 2161, 2345, 2380, 2416
NlaIV	GGNNCC	6 cut(s)	260, 546, 843, 2097, 2310, 2468
NmeAIII	GCCGAG	1 cut(s)	252
NmuCI	GTSAC	4 cut(s)	853, 1427, 1588, 2113
NsiI	ATGCAT	3 cut(s)	1090, 1330, 1645
NspI	RCATGY	1 cut(s)	605
PciI	ACATGT	1 cut(s)	601
PdmI	GAANNNNTTC	1 cut(s)	1959
PfeI	GAWTC	6 cut(s)	497, 592, 607, 2332, 2338, 2409
PflFI	GACNNNGTC	1 cut(s)	230
PkrI	GCNGC	9 cut(s)	62, 65, 238, 291, 329, 549, 1724, 1852, 2272
PleI	GAGTC	1 cut(s)	1790
PpsI	GAGTC	1 cut(s)	1790
Ppu21I	YACGTR	1 cut(s)	879
PscI	ACATGT	1 cut(s)	601
PshAI	GACNNNNGTC	1 cut(s)	2301
PshBI	ATTAAT	2 cut(s)	1458, 1818
PsiI	TTATAA	2 cut(s)	1187, 1806
PspEI	GGTNACC	1 cut(s)	2113
PspFI	CCCAGC	1 cut(s)	2487
PspN4I	GGNNCC	6 cut(s)	260, 546, 843, 2097, 2310, 2468
PspPI	GGNCC	3 cut(s)	258, 836, 2466
PsrI	GAACNNNNNNTAC	2 cut(s)	418, 450
PsuI	RGATCY	2 cut(s)	1152, 2095
PsyI	GACNNNGTC	1 cut(s)	230
RsaI	GTAC	7 cut(s)	372, 385, 600, 881, 1130, 1550, 2387
RsaNI	GTAC	7 cut(s)	371, 384, 599, 880, 1129, 1549, 2386
RseI	CAYNNNNRTG	1 cut(s)	1820
SatI	GCNGC	9 cut(s)	61, 64, 237, 290, 328, 548, 1723, 1851, 2271
Sau3AI	GATC	5 cut(s)	889, 1152, 2025, 2077, 2095
Sau96I	GGNCC	3 cut(s)	258, 836, 2466
SchI	GAGTC	1 cut(s)	1791
SduI	GDGCHC	1 cut(s)	2488
SfcI	CTRYAG	3 cut(s)	1513, 2119, 2292
SinI	GGWCC	3 cut(s)	258, 836, 2466
SmiMI	CAYNNNNRTG	1 cut(s)	1820
SmlI	CTYRAG	1 cut(s)	1069
SmoI	CTYRAG	1 cut(s)	1069
SspMI	CTAG	3 cut(s)	44, 1101, 1118
StyI	CCWWGG	2 cut(s)	366, 2376
TaaI	ACNGT	9 cut(s)	434, 721, 884, 983, 1168, 1178, 1600, 1992, 2390
TaiI	ACGT	2 cut(s)	881, 1595
TaqI	TCGA	7 cut(s)	252, 360, 716, 1018, 1609, 1780, 2028
TaqII	GACCGA	2 cut(s)	1226, 2454
TatI	WGTACW	3 cut(s)	598, 1128, 1548
TauI	GCSGC	6 cut(s)	63, 66, 239, 292, 330, 550
TfiI	GAWTC	6 cut(s)	497, 592, 607, 2332, 2338, 2409
TscAI	CASTG	5 cut(s)	726, 1171, 1183, 1997, 2070
TseFI	GTSAC	4 cut(s)	853, 1427, 1588, 2113
TseI	GCWGC	3 cut(s)	1722, 1850, 2270
Tsp45I	GTSAC	4 cut(s)	853, 1427, 1588, 2113
TspDTI	ATGAA	9 cut(s)	21, 701, 944, 1380, 1968, 2196, 2273, 2276, 2330
TspGWI	ACGGA	1 cut(s)	160
TspRI	CASTG	5 cut(s)	726, 1171, 1183, 1997, 2070
Tth111I	GACNNNGTC	1 cut(s)	230
VpaK11BI	GGWCC	3 cut(s)	258, 836, 2466
VspI	ATTAAT	2 cut(s)	1458, 1818
XapI	RAATTY	8 cut(s)	418, 439, 472, 567, 706, 1105, 1315, 2460
XceI	RCATGY	1 cut(s)	605
XmiI	GTMKAC	2 cut(s)	527, 1595
XmnI	GAANNNNTTC	1 cut(s)	1959
XspI	CTAG	3 cut(s)	44, 1101, 1118
Zsp2I	ATGCAT	3 cut(s)	1090, 1330, 1645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.