RchiOBHm_Chr7g0227311

pentatricopeptide repeat-containing protein At1g31840

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002491
Physical Location & Seq
Forward (+)
50591869 .. 50592286
418 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20359

Sequence Viewer

Length: 417 bp
ATGGTCAAGACTGGTTATGAACCTGATATTATTCTTTATGGTGTGCTATTAAATGGCCTTTGCAAGCATGGACTGATGAGTGACGCTCTTAGGTTCTTCTTTCAGGTTATTAATAGGGGTGTAAAGCCAAATGTTTATACTTTTAATATGTTGATAGATGGCTCCTGTAGATTGAATAGATTAAAAAATGCTGTGAAGGTGTACAACCAAATGCGAATGTATAACATAAGACCAGATGTGGTGACGTATACTGTGCTTATTCGAGGCATTTCTGAATTGGGGAGACTAAATGATGCATTAGTGTTTTTCTTCCAATCATTGAAGAGGGGTTTCTTGCCTGATGTTGTAACATATTGCACCCTCATTGATGCTTGCTGCAAGCATTTCTGCATGCTGGATTCCGGATTTTTGAGATGA

Protein Analysis

138

Amino Acids

15.88

Weight (kDa)

9.25

Isoelectric Point (pI)

28.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 1 - 52 1.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 5 - 33 1.4e-07 PPR repeat
PPR_2 PF13041 8 - 57 1.4e-14 PPR repeat family
PPR_1 PF12854 39 - 72 7.8e-13 PPR repeat
PPR_2 PF13041 48 - 90 1.2e-13 PPR repeat family
PPR_3 PF13812 67 - 126 7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 86 - 127 1.7e-08 PPR repeat family
PPR_1 PF12854 109 - 128 4.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 401
AfaI GTAC 1 cut(s) 203
AgsI TTSAA 2 cut(s) 175, 322
Alw26I GTCTC 1 cut(s) 277
Aor13HI TCCGGA 1 cut(s) 401
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 375
AseI ATTAAT 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 253
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 277
BfmI CTRYAG 1 cut(s) 166
BisI GCNGC 1 cut(s) 376
BlsI GCNGC 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 2 cut(s) 283, 358
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BsaWI WCCGGW 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 16
BseAI TCCGGA 1 cut(s) 401
BseNI ACTGG 1 cut(s) 16
BseXI GCAGC 1 cut(s) 362
BshFI GGCC 1 cut(s) 57
BsiSI CCGG 1 cut(s) 402
BsmAI GTCTC 1 cut(s) 277
BsnI GGCC 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 401
Bsp1407I TGTACA 1 cut(s) 201
BspANI GGCC 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 401
BspLI GGNNCC 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 1 cut(s) 16
BssNAI GTATAC 1 cut(s) 249
Bst1107I GTATAC 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 65, 373, 380, 392
BstDEI CTNAG 1 cut(s) 89
BstMAI GTCTC 1 cut(s) 277
BstNSI RCATGY 1 cut(s) 394
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 362
BstZ17I GTATAC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 57
Cac8I GCNNGC 4 cut(s) 65, 373, 380, 392
CseI GACGC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 202
CviAII CATG 2 cut(s) 68, 391
CviJI RGCY 3 cut(s) 57, 127, 162
CviKI_1 RGCY 3 cut(s) 57, 127, 162
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 298
FaeI CATG 2 cut(s) 71, 394
FatI CATG 2 cut(s) 67, 390
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 1 cut(s) 376
Fsp4HI GCNGC 1 cut(s) 376
GluI GCNGC 1 cut(s) 376
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 402
HgaI GACGC 1 cut(s) 92
Hin1II CATG 2 cut(s) 71, 394
HinfI GANTC 1 cut(s) 398
HpaII CCGG 1 cut(s) 402
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 202, 249
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 2 cut(s) 7, 402
Hpy8I GTNNAC 2 cut(s) 202, 249
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 245
HpyCH4V TGCA 5 cut(s) 63, 296, 357, 378, 390
HpyF3I CTNAG 1 cut(s) 89
HpySE526I ACGT 1 cut(s) 245
Hsp92II CATG 2 cut(s) 71, 394
Kpn2I TCCGGA 1 cut(s) 401
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 6 cut(s) 36, 89, 178, 246, 351, 380
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 2 cut(s) 283, 358
MaeII ACGT 1 cut(s) 245
MaeIII GTNAC 3 cut(s) 80, 241, 346
MboII GAAGA 3 cut(s) 88, 301, 334
MluCI AATT 1 cut(s) 275
MnlI CCTC 3 cut(s) 257, 318, 371
Mph1103I ATGCAT 1 cut(s) 298
MroI TCCGGA 1 cut(s) 401
MseI TTAA 4 cut(s) 50, 111, 144, 182
MspI CCGG 1 cut(s) 402
NlaIII CATG 2 cut(s) 71, 394
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 80, 241
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 1 cut(s) 394
PaeI GCATGC 1 cut(s) 394
PfeI GAWTC 1 cut(s) 398
PkrI GCNGC 1 cut(s) 377
PshBI ATTAAT 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 163
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SaqAI TTAA 4 cut(s) 50, 111, 144, 182
SatI GCNGC 1 cut(s) 376
SetI ASST 5 cut(s) 25, 95, 108, 201, 248
SfaNI GCATC 2 cut(s) 283, 358
SfcI CTRYAG 1 cut(s) 166
SphI GCATGC 1 cut(s) 394
Sse9I AATT 1 cut(s) 275
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 262
TasI AATT 1 cut(s) 275
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 4 cut(s) 50, 111, 144, 182
Tru9I TTAA 4 cut(s) 50, 111, 144, 182
TseFI GTSAC 2 cut(s) 80, 241
TseI GCWGC 1 cut(s) 375
Tsp45I GTSAC 2 cut(s) 80, 241
TspDTI ATGAA 1 cut(s) 33
VspI ATTAAT 1 cut(s) 111
XceI RCATGY 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.