Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0002491

Physical Location & Seq

Genomic Coordinates Reverse (-)

22182509 .. 22185145

Gene Structure 2637 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0241200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGACGGACTACCGGATACCTCCCACCATGAACCTCTGGACGGACGACAACGCGTCTCTCATGGAAGCCTTCATGAGCAACTCCGATCTCACCTCCTTCTGGGCCGCCCAGCCGGCCCAGCCCGCAACAACCCAACCCGCACACCCCCTCCACCAGCCTCAGTCCTCCGCTTCCACCTCCGACTATCCCCGCCCTCCGGCCGCGCAGTCGCAGCCTCCTCCGCCGCCGGCCGCGGCGCCGTTCAACCAGGAGACGCTTATGCAGCGGCTTCAGGCGCTGATCGACGGAGCCAGGGAGAGCTGGACCTACGCCATCTTCTGGCAGTCGTCGTACGAGATGTCCGGAGCTTCCGTGCTCGGATGGGGCGAGGGGTTCTACAAGGACGAGAGGGACAAGGTGAAAGCGAAGCCCAAAACGACGACGTCGTTGGTCGAGCAGGAGTACCGGAAGAAGGTGCTGAGAGACCTCAACTCTTTGATTTCCGGGGCCGACAACACCGCCGAGGACGACGTCGTGGATCAGGAAGTCACTGATACGGAATGGTTCTTCCTCGTTTCCATGACCCAGAACTTCGTCAACGGCGGCGGGTTGCCGGGTCAGGCTTTTTTTCAGTCCAACCCGGTCTGGGTCGCCGGCCCGGACCGCCTCGCCGCCTCGTCGTGCGAGCGGGCCAGACAAGGTCAGGTGTTCGGCCTACAGACTATGGTCTGCGTCCCGACGGCGAATGGGGTCGTCGAGTTGGGCTCGACGGAGCTTATATACCAGAGCGCGGATCTGATGAACAAGGTCCGAGTGTTGTTTGATTTCAATAACCTTGAAGTCGGTTCTTGGCCTATGTGTGGCGCCGCCGATCAGGGAGAGAACGACCCGTCGTCGCTGTGGATCAACGACAACCCGGCCGGCACCGGCACAATTGAAGTTGTCAAGGACTCAGTGCACATGGGTCCGGCGACTTCAGGACCCAGTACTAGTAACCATCAAATCTCGAAGCCGATTCAGTTCGACAGCCACCCTAGTTCGAGTGGCTTATCGGAGAATCCGAGCGCCGTTCTCCAAGTACAATCTCAGAGCAATCACCACCAGCAAGTACAGACGCAGAGCTTCTTCACCAGGGAGCTGAACTTCTCCGATTACAACGGCTACGACGGCAGCAGCGTCAAGAATTCGAATTCGAATTCTCATTCCTTGAAGCCGGAATCCGGCGAGATATTGAACTTCGGCGAGAGCAAGAGGACGTCTCACAGCGCCAACAACGGCAAGTTGTTTTCCGCCGAAGACAACAACAAAAAGAAGAGATCGCCGCCGTCGAGGGGCAGCAACGACGAGGGGATCCTGTCGTTTAGTTCGGGAGTGATTCTACCGTCGAGCAGCGGCGTCGTGAAATCGAGCGCCGGCCCAGTAGATTCCGACCACTCCGACCTCGAAGCCTCCGTGGCTAAGGAGGCCGACAGTAGCAGAGTTGTGGACCCGGAAAAACGACCCCGGAAAAGAGGTCGTAAACCGGCGAACGGCAGAGAGGAGCCGCTGAACCACGTGGAGGCGGAGAGACAGAGAAGAGAGAAGCTCAACCAGAGATTCTATGCTCTCCGAGCTGTCGTCCCAAATGTTTCGAAAATGGACAAGGCATCTCTGCTCGGAGACGCCATCTCTTACATCACCGAGCTCAAGACGAAGCTGCAAACGACGGAGTCCGACAAGGAGGACCTCCACAAGCAAGTCGAGACGTTAAGTAAAGAGCTGCAAGAGTCCCGATCGTCTCCGGGTTTGGACCAAGAGCTTAAAGGGTCTAATAAGTTGATCGATTTGGATATTGATGTCAAGATACTCGGATGGGACGCCAGGATTCAAGTTCAATGTAGCAAGAAGAACCACCCTGCGGCGAGACTAATGGCGGCGCTGATGGAGCTGGACTTGGATGTACACCATGCCAGCGTCTCGGTAGTCAATGATTTGATGATCCAGCAAGCCACCGTGAGGATGGGCAGCCGGATTTACACGCAGGAGCAGCTCAGGTTAGCTCTATCAGCCAAAGTGGGGGATGCCAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

682

Amino Acids

74.8

Weight (kDa)

5.51

Isoelectric Point (pI)

46.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	86 - 267	1.3e-54	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	510 - 556	9.9e-12	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	604 - 674	2.6e-07	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31840 AT1G31840
malus_domestica	MD12G1033400.v1.1
prunus_persica	Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G555500_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1 Prupe.1G569400_v2.0.a1
pyrus_communis	pycom12g03070
rosa_chinensis	RchiOBHm_Chr2g0139321 RchiOBHm_Chr7g0227311
rosa_laevigata	RLG00000001660
rosa_multiflora	Rmu_sc0001246.1_g000012 Rmu_sc0002525.1_g000013 Rmu_sc0003153.1_g000006 Rmu_sc0011922.1_g000005
rosa_roxburghii	Rroxscaffold_3G00232240
rosa_rugosa	Rorug07G0241200
rosa_samantha	Rh2BG417100 Rh7AG391100 Rh7AG391600 Rh7AG392200 Rh7AG392500 Rh7BG376100 Rh7CG192200 Rh7CG410100 Rh7CG410500 Rh7CG411300 Rh7CG411800 Rh7DG388700
rosa_wichuraiana	Rw7G032710 Rw7G032740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	425, 513, 1238
AccB1I	GGYRCC	3 cut(s)	235, 842, 902
AccB7I	CCANNNNNTGG	1 cut(s)	318
AccBSI	CCGCTC	1 cut(s)	667
AccII	CGCG	4 cut(s)	53, 203, 233, 770
AccIII	TCCGGA	1 cut(s)	341
AclWI	GGATC	6 cut(s)	525, 780, 890, 1324, 1337, 1951
AcoI	YGGCCR	3 cut(s)	198, 228, 897
AcsI	RAATTY	3 cut(s)	1162, 1168, 1174
AcuI	CTGAAG	2 cut(s)	254, 939
AcvI	CACGTG	1 cut(s)	1534
AcyI	GRCGYC	8 cut(s)	236, 422, 510, 843, 1235, 1374, 1641, 1836
AfaI	GTAC	6 cut(s)	332, 443, 967, 1059, 1089, 1920
AflIII	ACRYGT	1 cut(s)	51
AgsI	TTSAA	8 cut(s)	244, 808, 818, 917, 1189, 1213, 1847, 1853
AhdI	GACNNNNNGTC	2 cut(s)	52, 871
AhlI	ACTAGT	1 cut(s)	968
AjnI	CCWGG	4 cut(s)	246, 290, 1109, 1838
Alw21I	GWGCWC	3 cut(s)	357, 939, 1665
Alw44I	GTGCAC	1 cut(s)	935
AlwI	GGATC	6 cut(s)	525, 780, 890, 1324, 1337, 1951
AlwNI	CAGNNNCTG	1 cut(s)	277
Aor13HI	TCCGGA	1 cut(s)	341
ApaLI	GTGCAC	1 cut(s)	935
ApoI	RAATTY	3 cut(s)	1162, 1168, 1174
AspLEI	GCGC	9 cut(s)	205, 238, 277, 770, 845, 1046, 1247, 1391, 1897
AsuC2I	CCSGG	8 cut(s)	484, 594, 620, 638, 896, 1469, 1483, 1761
AsuHPI	GGTGA	5 cut(s)	82, 409, 1067, 1099, 1648
AsuII	TTCGAA	3 cut(s)	1166, 1172, 1610
AvaII	GGWCC	8 cut(s)	303, 640, 787, 944, 959, 1465, 1702, 1768
BaeGI	GKGCMC	1 cut(s)	939
BamHI	GGATCC	1 cut(s)	1329
BanI	GGYRCC	3 cut(s)	235, 842, 902
BanII	GRGCYC	2 cut(s)	746, 1665
BbrPI	CACGTG	1 cut(s)	1534
BbsI	GAAGAC	1 cut(s)	1281
Bbv12I	GWGCWC	3 cut(s)	357, 939, 1665
BccI	CCATC	7 cut(s)	320, 354, 984, 1652, 1824, 1894, 1972
BceAI	ACGGC	9 cut(s)	223, 595, 735, 1031, 1153, 1162, 1270, 1288, 1525
BcgI	CGANNNNNNTGC	2 cut(s)	1357, 1391
BciT130I	CCWGG	4 cut(s)	248, 292, 1111, 1840
BciVI	GTATCC	1 cut(s)	9
BcnI	CCSGG	8 cut(s)	484, 594, 620, 638, 896, 1469, 1483, 1761
BcuI	ACTAGT	1 cut(s)	968
BfaI	CTAG	2 cut(s)	969, 1014
BfmI	CTRYAG	1 cut(s)	695
BfoI	RGCGCY	7 cut(s)	239, 278, 846, 1047, 1248, 1392, 1898
BfuI	GTATCC	1 cut(s)	9
BglI	GCCNNNNNGGC	2 cut(s)	113, 1433
BmcAI	AGTACT	1 cut(s)	967
Bme18I	GGWCC	8 cut(s)	303, 640, 787, 944, 959, 1465, 1702, 1768
BmeRI	GACNNNNNGTC	2 cut(s)	52, 871
BmrI	ACTGGG	2 cut(s)	957, 1391
BmsI	GCATC	2 cut(s)	1634, 2029
BmuI	ACTGGG	2 cut(s)	957, 1391
BoxI	GACNNNNGTC	1 cut(s)	704
BpiI	GAAGAC	1 cut(s)	1281
BplI	GAGNNNNNCTC	6 cut(s)	728, 760, 1222, 1254, 1548, 1580
Bpu10I	CCTNAGC	2 cut(s)	1437, 2009
Bpu14I	TTCGAA	3 cut(s)	1166, 1172, 1610
BpuEI	CTTGAG	1 cut(s)	1649
BpuMI	CCSGG	8 cut(s)	484, 594, 620, 638, 896, 1469, 1483, 1761
Bsa29I	ATCGAT	1 cut(s)	1800
BsaAI	YACGTR	1 cut(s)	1534
BsaHI	GRCGYC	8 cut(s)	236, 422, 510, 843, 1235, 1374, 1641, 1836
BsaI	GGTCTC	1 cut(s)	456
BsaJI	CCNNGG	7 cut(s)	231, 291, 483, 501, 1110, 1431, 1481
BsaWI	WCCGGW	3 cut(s)	12, 341, 444
BsaXI	ACNNNNNCTCC	4 cut(s)	132, 162, 336, 366
Bse118I	RCCGGY	7 cut(s)	112, 226, 632, 899, 905, 1391, 1501
Bse1I	ACTGG	2 cut(s)	963, 1397
BseAI	TCCGGA	1 cut(s)	341
BseBI	CCWGG	4 cut(s)	248, 292, 1111, 1840
BseCI	ATCGAT	1 cut(s)	1800
BseDI	CCNNGG	7 cut(s)	231, 291, 483, 501, 1110, 1431, 1481
BseGI	GGATG	5 cut(s)	365, 1835, 1921, 1983, 2044
BseMII	CTCAG	5 cut(s)	173, 449, 945, 1079, 2023
BseNI	ACTGG	2 cut(s)	963, 1397
BseRI	GAGGAG	2 cut(s)	207, 1532
BseSI	GKGCMC	1 cut(s)	939
BseX3I	CGGCCG	3 cut(s)	198, 228, 897
BseYI	CCCAGC	2 cut(s)	108, 117
Bsh1236I	CGCG	4 cut(s)	53, 203, 233, 770
Bsh1285I	CGRYCG	4 cut(s)	201, 231, 900, 1754
BshNI	GGYRCC	3 cut(s)	235, 842, 902
BshVI	ATCGAT	1 cut(s)	1800
BsiEI	CGRYCG	4 cut(s)	201, 231, 900, 1754
BsiHKAI	GWGCWC	3 cut(s)	357, 939, 1665
BsiWI	CGTACG	1 cut(s)	330
BslFI	GGGAC	5 cut(s)	404, 698, 1583, 1732, 1847
BsmBI	CGTCTC	7 cut(s)	60, 245, 1242, 1632, 1715, 1761, 1939
BsmFI	GGGAC	5 cut(s)	404, 698, 1583, 1732, 1847
Bso31I	GGTCTC	1 cut(s)	456
Bsp119I	TTCGAA	3 cut(s)	1166, 1172, 1610
Bsp1286I	GDGCHC	4 cut(s)	357, 746, 939, 1665
Bsp13I	TCCGGA	1 cut(s)	341
Bsp1407I	TGTACA	1 cut(s)	1918
BspCNI	CTCAG	5 cut(s)	172, 450, 944, 1078, 2022
BspDI	ATCGAT	1 cut(s)	1800
BspEI	TCCGGA	1 cut(s)	341
BspFNI	CGCG	4 cut(s)	53, 203, 233, 770
BspHI	TCATGA	1 cut(s)	72
BspPI	GGATC	6 cut(s)	525, 780, 890, 1324, 1337, 1951
BspT104I	TTCGAA	3 cut(s)	1166, 1172, 1610
BspT107I	GGYRCC	3 cut(s)	235, 842, 902
BspTNI	GGTCTC	1 cut(s)	456
BsrBI	CCGCTC	1 cut(s)	667
BsrFI	RCCGGY	7 cut(s)	112, 226, 632, 899, 905, 1391, 1501
BsrGI	TGTACA	1 cut(s)	1918
BsrI	ACTGG	2 cut(s)	963, 1397
BssAI	RCCGGY	7 cut(s)	112, 226, 632, 899, 905, 1391, 1501
BssECI	CCNNGG	7 cut(s)	231, 291, 483, 501, 1110, 1431, 1481
BssNI	GRCGYC	8 cut(s)	236, 422, 510, 843, 1235, 1374, 1641, 1836
Bst2UI	CCWGG	4 cut(s)	248, 292, 1111, 1840
Bst4CI	ACNGT	3 cut(s)	1362, 1451, 1972
Bst6I	CTCTTC	2 cut(s)	1286, 1549
BstACI	GRCGYC	8 cut(s)	236, 422, 510, 843, 1235, 1374, 1641, 1836
BstAUI	TGTACA	1 cut(s)	1918
BstBAI	YACGTR	1 cut(s)	1534
BstBI	TTCGAA	3 cut(s)	1166, 1172, 1610
BstDEI	CTNAG	6 cut(s)	159, 458, 931, 1065, 1437, 2009
BstDSI	CCRYGG	2 cut(s)	231, 1431
BstF5I	GGATG	5 cut(s)	365, 1835, 1921, 1983, 2044
BstFNI	CGCG	4 cut(s)	53, 203, 233, 770
BstH2I	RGCGCY	7 cut(s)	239, 278, 846, 1047, 1248, 1392, 1898
BstHHI	GCGC	9 cut(s)	205, 238, 277, 770, 845, 1046, 1247, 1391, 1897
BstMCI	CGRYCG	4 cut(s)	201, 231, 900, 1754
BstNI	CCWGG	4 cut(s)	248, 292, 1111, 1840
BstPAI	GACNNNNGTC	1 cut(s)	704
BstSFI	CTRYAG	1 cut(s)	695
BstSLI	GKGCMC	1 cut(s)	939
BstUI	CGCG	4 cut(s)	53, 203, 233, 770
BstV2I	GAAGAC	1 cut(s)	1281
BstX2I	RGATCY	2 cut(s)	772, 1329
BstYI	RGATCY	2 cut(s)	772, 1329
BstZI	CGGCCG	3 cut(s)	198, 228, 897
Bsu15I	ATCGAT	1 cut(s)	1800
BsuI	GTATCC	1 cut(s)	9
BsuTUI	ATCGAT	1 cut(s)	1800
BtgI	CCRYGG	2 cut(s)	231, 1431
BtsCI	GGATG	5 cut(s)	365, 1835, 1921, 1983, 2044
BtsIMutI	CAGTG	2 cut(s)	528, 939
CaiI	CAGNNNCTG	1 cut(s)	277
CciI	TCATGA	1 cut(s)	72
CfoI	GCGC	9 cut(s)	205, 238, 277, 770, 845, 1046, 1247, 1391, 1897
Cfr10I	RCCGGY	7 cut(s)	112, 226, 632, 899, 905, 1391, 1501
Cfr42I	CCGCGG	1 cut(s)	234
ClaI	ATCGAT	1 cut(s)	1800
CpoI	CGGWCCG	1 cut(s)	640
CseI	GACGC	9 cut(s)	42, 262, 700, 1102, 1144, 1363, 1649, 1844, 1921
Csp6I	GTAC	6 cut(s)	331, 442, 966, 1058, 1088, 1919
CspI	CGGWCCG	1 cut(s)	640
CviAII	CATG	6 cut(s)	28, 61, 73, 559, 940, 1925
CviQI	GTAC	6 cut(s)	331, 442, 966, 1058, 1088, 1919
DdeI	CTNAG	6 cut(s)	159, 458, 931, 1065, 1437, 2009
DinI	GGCGCC	2 cut(s)	237, 844
DriI	GACNNNNNGTC	2 cut(s)	52, 871
EaeI	YGGCCR	3 cut(s)	198, 228, 897
EagI	CGGCCG	3 cut(s)	198, 228, 897
Eam1104I	CTCTTC	2 cut(s)	1286, 1549
Eam1105I	GACNNNNNGTC	2 cut(s)	52, 871
EarI	CTCTTC	2 cut(s)	1286, 1549
EciI	GGCGGA	3 cut(s)	210, 1258, 1556
Ecl136II	GAGCTC	1 cut(s)	1663
EclXI	CGGCCG	3 cut(s)	198, 228, 897
Eco24I	GRGCYC	2 cut(s)	746, 1665
Eco31I	GGTCTC	1 cut(s)	456
Eco47I	GGWCC	8 cut(s)	303, 640, 787, 944, 959, 1465, 1702, 1768
Eco52I	CGGCCG	3 cut(s)	198, 228, 897
Eco53kI	GAGCTC	1 cut(s)	1663
Eco57I	CTGAAG	2 cut(s)	254, 939
Eco72I	CACGTG	1 cut(s)	1534
EcoICRI	GAGCTC	1 cut(s)	1663
EcoO109I	RGGNCCY	2 cut(s)	959, 1702
EcoRI	GAATTC	3 cut(s)	1162, 1168, 1174
EcoRII	CCWGG	4 cut(s)	246, 290, 1109, 1838
EcoT38I	GRGCYC	2 cut(s)	746, 1665
EgeI	GGCGCC	2 cut(s)	237, 844
EheI	GGCGCC	2 cut(s)	237, 844
Esp3I	CGTCTC	7 cut(s)	60, 245, 1242, 1632, 1715, 1761, 1939
FaeI	CATG	6 cut(s)	31, 64, 76, 562, 943, 1928
FaqI	GGGAC	5 cut(s)	404, 698, 1583, 1732, 1847
FatI	CATG	6 cut(s)	27, 60, 72, 558, 939, 1924
FauI	CCCGC	5 cut(s)	130, 145, 197, 578, 660
FokI	GGATG	4 cut(s)	372, 1842, 1928, 1990
FriOI	GRGCYC	2 cut(s)	746, 1665
FspBI	CTAG	2 cut(s)	969, 1014
GlaI	GCGC	9 cut(s)	204, 237, 276, 769, 844, 1045, 1246, 1390, 1896
GsaI	CCCAGC	2 cut(s)	112, 121
HaeII	RGCGCY	7 cut(s)	239, 278, 846, 1047, 1248, 1392, 1898
HgaI	GACGC	9 cut(s)	42, 262, 700, 1102, 1144, 1363, 1649, 1844, 1921
HhaI	GCGC	9 cut(s)	205, 238, 277, 770, 845, 1046, 1247, 1391, 1897
Hin1I	GRCGYC	8 cut(s)	236, 422, 510, 843, 1235, 1374, 1641, 1836
Hin1II	CATG	6 cut(s)	31, 64, 76, 562, 943, 1928
Hin6I	GCGC	9 cut(s)	203, 236, 275, 768, 843, 1044, 1245, 1389, 1895
HinP1I	GCGC	9 cut(s)	203, 236, 275, 768, 843, 1044, 1245, 1389, 1895
HincII	GTYRAC	1 cut(s)	577
HindII	GTYRAC	1 cut(s)	577
HphI	GGTGA	5 cut(s)	82, 409, 1067, 1099, 1648
Hpy166II	GTNNAC	5 cut(s)	577, 937, 1465, 1499, 1921
Hpy8I	GTNNAC	5 cut(s)	577, 937, 1465, 1499, 1921
HpyAV	CCTTC	3 cut(s)	79, 106, 445
HpyCH4III	ACNGT	3 cut(s)	1362, 1451, 1972
HpyCH4IV	ACGT	5 cut(s)	422, 510, 1235, 1533, 1724
HpyCH4V	TGCA	4 cut(s)	262, 937, 1678, 1741
HpyF3I	CTNAG	6 cut(s)	159, 458, 931, 1065, 1437, 2009
HpySE526I	ACGT	5 cut(s)	422, 510, 1235, 1533, 1724
Hsp92I	GRCGYC	8 cut(s)	236, 422, 510, 843, 1235, 1374, 1641, 1836
Hsp92II	CATG	6 cut(s)	31, 64, 76, 562, 943, 1928
HspAI	GCGC	9 cut(s)	203, 236, 275, 768, 843, 1044, 1245, 1389, 1895
KasI	GGCGCC	2 cut(s)	235, 842
Kpn2I	TCCGGA	1 cut(s)	341
KroI	GCCGGC	5 cut(s)	112, 226, 632, 899, 1391
KroNI	GCCGGC	5 cut(s)	114, 228, 634, 901, 1393
KspI	CCGCGG	1 cut(s)	234
LmnI	GCTCC	7 cut(s)	287, 344, 751, 1114, 1519, 1903, 2002
LweI	GCATC	2 cut(s)	1634, 2029
MaeI	CTAG	2 cut(s)	969, 1014
MaeII	ACGT	5 cut(s)	422, 510, 1235, 1533, 1724
MaeIII	GTNAC	2 cut(s)	526, 971
MbiI	CCGCTC	1 cut(s)	667
MboII	GAAGA	8 cut(s)	307, 460, 538, 1096, 1286, 1303, 1566, 1876
MfeI	CAATTG	1 cut(s)	912
MflI	RGATCY	2 cut(s)	772, 1329
MhlI	GDGCHC	4 cut(s)	357, 746, 939, 1665
MluCI	AATT	4 cut(s)	912, 1162, 1168, 1174
MluI	ACGCGT	1 cut(s)	51
Mly113I	GGCGCC	2 cut(s)	236, 843
MlyI	GAGTC	3 cut(s)	923, 1697, 1754
MmeI	TCCRAC	5 cut(s)	204, 639, 1431, 1440, 1716
MroI	TCCGGA	1 cut(s)	341
MroNI	GCCGGC	5 cut(s)	112, 226, 632, 899, 1391
MseI	TTAA	2 cut(s)	1727, 1779
MspA1I	CMGCKG	4 cut(s)	233, 265, 1371, 1525
MunI	CAATTG	1 cut(s)	912
MvaI	CCWGG	4 cut(s)	248, 292, 1111, 1840
MvnI	CGCG	4 cut(s)	53, 203, 233, 770
NaeI	GCCGGC	5 cut(s)	114, 228, 634, 901, 1393
NarI	GGCGCC	2 cut(s)	236, 843
NciI	CCSGG	8 cut(s)	484, 594, 620, 638, 896, 1469, 1483, 1761
NgoMIV	GCCGGC	5 cut(s)	112, 226, 632, 899, 1391
NlaIII	CATG	6 cut(s)	31, 64, 76, 562, 943, 1928
NmeAIII	GCCGAG	1 cut(s)	526
NmuCI	GTSAC	1 cut(s)	526
NspV	TTCGAA	3 cut(s)	1166, 1172, 1610
PagI	TCATGA	1 cut(s)	72
PcsI	WCGNNNNNNNCGW	6 cut(s)	363, 422, 1037, 1152, 1304, 1428
PdiI	GCCGGC	5 cut(s)	114, 228, 634, 901, 1393
PfeI	GAWTC	7 cut(s)	994, 1036, 1196, 1354, 1403, 1575, 1843
Pfl23II	CGTACG	1 cut(s)	330
PflFI	GACNNNGTC	4 cut(s)	421, 509, 678, 1687
PflMI	CCANNNNNTGG	1 cut(s)	318
Ple19I	CGATCG	1 cut(s)	1754
PleI	GAGTC	3 cut(s)	923, 1696, 1753
PluTI	GGCGCC	2 cut(s)	239, 846
PmaCI	CACGTG	1 cut(s)	1534
PmlI	CACGTG	1 cut(s)	1534
PpsI	GAGTC	3 cut(s)	923, 1696, 1753
Ppu21I	YACGTR	1 cut(s)	1534
PpuMI	RGGWCCY	2 cut(s)	959, 1702
PshAI	GACNNNNGTC	1 cut(s)	704
Psp124BI	GAGCTC	1 cut(s)	1665
Psp5II	RGGWCCY	2 cut(s)	959, 1702
Psp6I	CCWGG	4 cut(s)	246, 290, 1109, 1838
PspCI	CACGTG	1 cut(s)	1534
PspFI	CCCAGC	2 cut(s)	108, 117
PspGI	CCWGG	4 cut(s)	246, 290, 1109, 1838
PspLI	CGTACG	1 cut(s)	330
PspPPI	RGGWCCY	2 cut(s)	959, 1702
PstNI	CAGNNNCTG	1 cut(s)	277
PsuI	RGATCY	2 cut(s)	772, 1329
PsyI	GACNNNGTC	4 cut(s)	421, 509, 678, 1687
PvuI	CGATCG	1 cut(s)	1754
RsaI	GTAC	6 cut(s)	332, 443, 967, 1059, 1089, 1920
RsaNI	GTAC	6 cut(s)	331, 442, 966, 1058, 1088, 1919
Rsr2I	CGGWCCG	1 cut(s)	640
RsrII	CGGWCCG	1 cut(s)	640
SacI	GAGCTC	1 cut(s)	1665
SacII	CCGCGG	1 cut(s)	234
SaqAI	TTAA	2 cut(s)	1727, 1779
ScaI	AGTACT	1 cut(s)	967
SchI	GAGTC	3 cut(s)	923, 1697, 1754
SduI	GDGCHC	4 cut(s)	357, 746, 939, 1665
SfaNI	GCATC	2 cut(s)	1634, 2029
SfcI	CTRYAG	1 cut(s)	695
SfoI	GGCGCC	2 cut(s)	237, 844
Sfr303I	CCGCGG	1 cut(s)	234
SfuI	TTCGAA	3 cut(s)	1166, 1172, 1610
SgrBI	CCGCGG	1 cut(s)	234
SinI	GGWCC	8 cut(s)	303, 640, 787, 944, 959, 1465, 1702, 1768
SmlI	CTYRAG	1 cut(s)	1664
SmoI	CTYRAG	1 cut(s)	1664
SpeI	ACTAGT	1 cut(s)	968
Sse9I	AATT	4 cut(s)	912, 1162, 1168, 1174
SspDI	GGCGCC	2 cut(s)	235, 842
SspMI	CTAG	2 cut(s)	969, 1014
SstI	GAGCTC	1 cut(s)	1665
TaaI	ACNGT	3 cut(s)	1362, 1451, 1972
TaiI	ACGT	5 cut(s)	425, 513, 1238, 1536, 1727
TasI	AATT	4 cut(s)	912, 1162, 1168, 1174
TatI	WGTACW	4 cut(s)	965, 1057, 1087, 1918
TfiI	GAWTC	7 cut(s)	994, 1036, 1196, 1354, 1403, 1575, 1843
Tru1I	TTAA	2 cut(s)	1727, 1779
Tru9I	TTAA	2 cut(s)	1727, 1779
TscAI	CASTG	2 cut(s)	535, 939
TseFI	GTSAC	1 cut(s)	526
Tsp45I	GTSAC	1 cut(s)	526
TspDTI	ATGAA	3 cut(s)	44, 61, 794
TspGWI	ACGGA	8 cut(s)	20, 56, 300, 340, 551, 764, 1420, 1700
TspRI	CASTG	2 cut(s)	535, 939
Tth111I	GACNNNGTC	4 cut(s)	421, 509, 678, 1687
Van91I	CCANNNNNTGG	1 cut(s)	318
VneI	GTGCAC	1 cut(s)	935
VpaK11BI	GGWCC	8 cut(s)	303, 640, 787, 944, 959, 1465, 1702, 1768
XapI	RAATTY	3 cut(s)	1162, 1168, 1174
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1975
XspI	CTAG	2 cut(s)	969, 1014
ZraI	GACGTC	3 cut(s)	423, 511, 1236
ZrmI	AGTACT	1 cut(s)	967

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.